AGO1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO1
Entrez Gene ID	522972
Full Name	argonaute 1, RISC catalytic component
Synonyms	EIF2C1
General protein information	Preferred Names argonaute 1, RISC catalytic component Names protein argonaute-1 eukaryotic translation initiation factor 2C, 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317187.1	NP_001304116.1	protein argonaute-1 isoform 1x
NM_001317187.2	NP_001304116.1	protein argonaute-1 isoform 1x
XM_010803634.3	XP_010801936.1	protein argonaute-1 isoform X1
XM_010803634.2	XP_010801936.1	protein argonaute-1 isoform X1
NM_001205899.2	NP_001192828.1	protein argonaute-1 isoform 1
NM_001205899.3	NP_001192828.1	protein argonaute-1 isoform 1
XM_024989359.1	XP_024845127.1	protein argonaute-1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570775	XP_005158529.1
Gallus gallus (chicken)	EIF2C1	XP_425781.3
Xenopus tropicalis (tropical clawed frog)	ago4	XP_002936260.1
Homo sapiens (human)	AGO1	NP_036331.1
Canis lupus familiaris (dog)	AGO1	XP_532563.3
Mus musculus (house mouse)	Ago1	NP_700452.2
Rattus norvegicus (Norway rat)	Ago1	NP_001178694.1
Bos taurus (cattle)	EIF2C1	NP_001192828.1
Pan troglodytes (chimpanzee)	EIF2C1	XP_513312.2

Gene Ontology
Bibliography

Related articles in PubMed

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40897	NM_001205899.3 Latest version!	Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa50628	XM_010803634.3 Latest version!	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa95433	NM_001317187.2 Latest version!	Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa232780	XM_024989359.1 Latest version!	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa95433	NM_001317187.1	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa50628	XM_010803634.2	Bos taurus argonaute RISC catalytic component 1 (AGO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa40897	NM_001205899.2	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO1 ( NM_001205899.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192828.1 Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa40897
Clone ID Related Accession (Same CDS sequence)	NM_001205899.2 , NM_001205899.3
Accession Version	NM_001205899.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-19
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE913054.1, DAAA02009205.1, EE913055.1, EE347213.1, EH183084.1, EE903399.1 and CO875103.1. On Nov 19, 2015 this sequence version replaced NM_001205899.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192828.1
CDS	179..2752
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_001205899.2
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205899.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_001205899.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192828.1 Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa40897
Clone ID Related Accession (Same CDS sequence)	NM_001205899.2 , NM_001205899.3
Accession Version	NM_001205899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE913054.1, NKLS02000003.1, EE913055.1, EE347213.1, EH183084.1, EE903399.1 and CO875103.1. On May 25, 2018 this sequence version replaced NM_001205899.2. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192828.1
CDS	179..2752
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_001205899.3
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205899.3

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( XM_010803634.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801936.1 Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50628
Clone ID Related Accession (Same CDS sequence)	XM_010803634.3 , XM_010803634.2
Accession Version	XM_010803634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCCACA CCCAATCAGG GTGTCTGGGA CATGCGGGGG 
AAACAGTTCT ACAATGGCAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAGTGTC GAGAAGAAGT GCTCAAGAAC TTCACGGACC AGCTGCGGAA GATTTCCAAG 
GACGCAGGGA TGCCCATCCA GGGCCAGCCT TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGTGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGGCTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTTGG AGATACTCTG 
TTGGGAATGG CTACACAGTG TGTGCAAGTG AAGAACGTGG TCAAGACCTC GCCTCAGACT 
CTGTCTAACC TTTGCCTGAA GATCAATGTC AAACTTGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCAGT CTTTCAACAA CCAGTGATAT TCCTGGGAGC AGATGTTACA 
CACCCACCAG CAGGGGATGG GAAAAAACCC TCTATCACAG CAGTGGTAGG CAGCATGGAT 
GCCCACCCCA GCCGTTACTG TGCTACTGTG CGGGTACAGC GACCACGGCA GGAGATCATT 
GAAGACTTAT CCTACATGGT ACGTGAGCTG CTCATCCAGT TCTACAAGTC CACGCGCTTC 
AAGCCTACCC GCATCATCTT CTACCGAGAT GGGGTTCCTG AAGGCCAGCT TCCCCAGATC 
CTCCACTATG AGCTGCTGGC CATTCGTGAT GCCTGCATCA AACTGGAAAA GGACTACCAG 
CCCGGAATCA CTTATATTGT GGTGCAGAAA CGCCATCACA CCCGCCTTTT TTGTGCTGAC 
AAGAATGAGC GAATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT TGACTTCTAT CTGTGCAGCC ACGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTACTA TGTCCTTTGG GATGACAACC GTTTCACAGC GGATGAGCTC 
CAGATCTTGA CGTACCAGCT GTGCCACACT TACGTACGAT GCACACGCTC CGTCTCAATT 
CCAGCACCTG CCTACTATGC CCGCTTGGTG GCTTTCCGGG CACGATACCA CCTAGTGGAC 
AAAGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCGGGGC AGAGCAATGG GCGGGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TGCGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801936.1
CDS	174..2756
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_010803634.3
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803634.3

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCCACA CCCAATCAGG GTGTCTGGGA CATGCGGGGG 
AAACAGTTCT ACAATGGCAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAGTGTC GAGAAGAAGT GCTCAAGAAC TTCACGGACC AGCTGCGGAA GATTTCCAAG 
GACGCAGGGA TGCCCATCCA GGGCCAGCCT TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGTGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGGCTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTTGG AGATACTCTG 
TTGGGAATGG CTACACAGTG TGTGCAAGTG AAGAACGTGG TCAAGACCTC GCCTCAGACT 
CTGTCTAACC TTTGCCTGAA GATCAATGTC AAACTTGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCAGT CTTTCAACAA CCAGTGATAT TCCTGGGAGC AGATGTTACA 
CACCCACCAG CAGGGGATGG GAAAAAACCC TCTATCACAG CAGTGGTAGG CAGCATGGAT 
GCCCACCCCA GCCGTTACTG TGCTACTGTG CGGGTACAGC GACCACGGCA GGAGATCATT 
GAAGACTTAT CCTACATGGT ACGTGAGCTG CTCATCCAGT TCTACAAGTC CACGCGCTTC 
AAGCCTACCC GCATCATCTT CTACCGAGAT GGGGTTCCTG AAGGCCAGCT TCCCCAGATC 
CTCCACTATG AGCTGCTGGC CATTCGTGAT GCCTGCATCA AACTGGAAAA GGACTACCAG 
CCCGGAATCA CTTATATTGT GGTGCAGAAA CGCCATCACA CCCGCCTTTT TTGTGCTGAC 
AAGAATGAGC GAATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT TGACTTCTAT CTGTGCAGCC ACGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTACTA TGTCCTTTGG GATGACAACC GTTTCACAGC GGATGAGCTC 
CAGATCTTGA CGTACCAGCT GTGCCACACT TACGTACGAT GCACACGCTC CGTCTCAATT 
CCAGCACCTG CCTACTATGC CCGCTTGGTG GCTTTCCGGG CACGATACCA CCTAGTGGAC 
AAAGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCGGGGC AGAGCAATGG GCGGGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TGCGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( XM_010803634.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801936.1 Bos taurus argonaute RISC catalytic component 1 (AGO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50628
Clone ID Related Accession (Same CDS sequence)	XM_010803634.3 , XM_010803634.2
Accession Version	XM_010803634.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCCACA CCCAATCAGG GTGTCTGGGA CATGCGGGGG 
AAACAGTTCT ACAATGGCAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAGTGTC GAGAAGAAGT GCTCAAGAAC TTCACGGACC AGCTGCGGAA GATTTCCAAG 
GACGCAGGGA TGCCCATCCA GGGCCAGCCT TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGTGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGGCTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTTGG AGATACTCTG 
TTGGGAATGG CTACACAGTG TGTGCAAGTG AAGAACGTGG TCAAGACCTC GCCTCAGACT 
CTGTCTAACC TTTGCCTGAA GATCAATGTC AAACTTGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCAGT CTTTCAACAA CCAGTGATAT TCCTGGGAGC AGATGTTACA 
CACCCACCAG CAGGGGATGG GAAAAAACCC TCTATCACAG CAGTGGTAGG CAGCATGGAT 
GCCCACCCCA GCCGTTACTG TGCTACTGTG CGGGTACAGC GACCACGGCA GGAGATCATT 
GAAGACTTAT CCTACATGGT ACGTGAGCTG CTCATCCAGT TCTACAAGTC CACGCGCTTC 
AAGCCTACCC GCATCATCTT CTACCGAGAT GGGGTTCCTG AAGGCCAGCT TCCCCAGATC 
CTCCACTATG AGCTGCTGGC CATTCGTGAT GCCTGCATCA AACTGGAAAA GGACTACCAG 
CCCGGAATCA CTTATATTGT GGTGCAGAAA CGCCATCACA CCCGCCTTTT TTGTGCTGAC 
AAGAATGAGC GAATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT TGACTTCTAT CTGTGCAGCC ACGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTACTA TGTCCTTTGG GATGACAACC GTTTCACAGC GGATGAGCTC 
CAGATCTTGA CGTACCAGCT GTGCCACACT TACGTACGAT GCACACGCTC CGTCTCAATT 
CCAGCACCTG CCTACTATGC CCGCTTGGTG GCTTTCCGGG CACGATACCA CCTAGTGGAC 
AAAGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCGGGGC AGAGCAATGG GCGGGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TGCGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801936.1
CDS	174..2756
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_010803634.2
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute RISC catalytic component 1 (AGO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803634.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCCACA CCCAATCAGG GTGTCTGGGA CATGCGGGGG 
AAACAGTTCT ACAATGGCAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAGTGTC GAGAAGAAGT GCTCAAGAAC TTCACGGACC AGCTGCGGAA GATTTCCAAG 
GACGCAGGGA TGCCCATCCA GGGCCAGCCT TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGTGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGGCTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTTGG AGATACTCTG 
TTGGGAATGG CTACACAGTG TGTGCAAGTG AAGAACGTGG TCAAGACCTC GCCTCAGACT 
CTGTCTAACC TTTGCCTGAA GATCAATGTC AAACTTGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCAGT CTTTCAACAA CCAGTGATAT TCCTGGGAGC AGATGTTACA 
CACCCACCAG CAGGGGATGG GAAAAAACCC TCTATCACAG CAGTGGTAGG CAGCATGGAT 
GCCCACCCCA GCCGTTACTG TGCTACTGTG CGGGTACAGC GACCACGGCA GGAGATCATT 
GAAGACTTAT CCTACATGGT ACGTGAGCTG CTCATCCAGT TCTACAAGTC CACGCGCTTC 
AAGCCTACCC GCATCATCTT CTACCGAGAT GGGGTTCCTG AAGGCCAGCT TCCCCAGATC 
CTCCACTATG AGCTGCTGGC CATTCGTGAT GCCTGCATCA AACTGGAAAA GGACTACCAG 
CCCGGAATCA CTTATATTGT GGTGCAGAAA CGCCATCACA CCCGCCTTTT TTGTGCTGAC 
AAGAATGAGC GAATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT TGACTTCTAT CTGTGCAGCC ACGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTACTA TGTCCTTTGG GATGACAACC GTTTCACAGC GGATGAGCTC 
CAGATCTTGA CGTACCAGCT GTGCCACACT TACGTACGAT GCACACGCTC CGTCTCAATT 
CCAGCACCTG CCTACTATGC CCGCTTGGTG GCTTTCCGGG CACGATACCA CCTAGTGGAC 
AAAGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCGGGGC AGAGCAATGG GCGGGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TGCGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_001317187.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001304116.1 Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95433
Clone ID Related Accession (Same CDS sequence)	NM_001317187.1 , NM_001317187.2
Accession Version	NM_001317187.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-19
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE913054.1, DAAA02009205.1, EE913055.1, EE347213.1, EH183084.1, EE903399.1 and CO875103.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAAGAGCT GTGCCGCCCA 
AATCCAGAGG AAGCAGGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304116.1
CDS	179..2854
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*RQNAVTSLDRRKLSKPQELCRPNPEEAGRREVG

Target ORF information:

RefSeq Version	NM_001317187.1
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317187.1

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAAGAGCT GTGCCGCCCA 
AATCCAGAGG AAGCAGGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_001317187.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001304116.1 Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95433
Clone ID Related Accession (Same CDS sequence)	NM_001317187.1 , NM_001317187.2
Accession Version	NM_001317187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE913054.1, NKLS02000003.1, EE913055.1, EE347213.1, EH183084.1, EE903399.1 and CO875103.1. On May 25, 2018 this sequence version replaced NM_001317187.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAAGAGCT GTGCCGCCCA 
AATCCAGAGG AAGCAGGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304116.1
CDS	179..2854
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*RQNAVTSLDRRKLSKPQELCRPNPEEAGRREVG

Target ORF information:

RefSeq Version	NM_001317187.2
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317187.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGCGCCTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AAGTGGACAT CCCTAAGATT GACGTCTATC ACTACGAAGT GGATATCAAG 
CCAGATAAGT GTCCTCGCAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TATTTGGTGA CCGCAAGCCC GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAATGAACGG GTTGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTCAA GGTCTCCATC AAGTGGCTGG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTTCCCC TGGAGTCTGT ACAAGCCCTG 
GATGTAGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AGGCACAGCC AGTGATTGAG TTCATGTGTG AGGTGCTCGA CATCAGGAAC 
ATAGATGAGC AGCCCAAAGC CCTCACGGAC TCTCAGCGTG TGCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC CAGCCATCAG ACGTTTCCTC TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC CCAGTATTTC AAGCAGAAAT ATAACCTGCA GCTCAAGTAT 
CCCCACCTGC CTTGCCTGCA AGTTGGCCAG GAACAAAAGC ATACCTACCT GCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGCTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGATCGGCC CCAGACAGAC AAGAGGAGAT TAGCCGCCTG 
ATGAAGAATG CCAGCTACAA CCTAGATCCG TACATCCAGG AATTTGGGAT CAAGGTGAAG 
GATGACATGA CGGAGGTGAC GGGACGAGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGCA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAAG TGCTCAAGAA CTTCACGGAC CAGCTGCGGA AGATTTCCAA GGACGCAGGG 
ATGCCCATCC AGGGCCAGCC TTGCTTCTGC AAATACGCAC AGGGGGCAGA CAGTGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTTG GAGATACTCT GTTGGGAATG 
GCTACACAGT GTGTGCAAGT GAAGAACGTG GTCAAGACCT CGCCTCAGAC TCTGTCTAAC 
CTTTGCCTGA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCAG TCTTTCAACA ACCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCACCA 
GCAGGGGATG GGAAAAAACC CTCTATCACA GCAGTGGTAG GCAGCATGGA TGCCCACCCC 
AGCCGTTACT GTGCTACTGT GCGGGTACAG CGACCACGGC AGGAGATCAT TGAAGACTTA 
TCCTACATGG TACGTGAGCT GCTCATCCAG TTCTACAAGT CCACGCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTTCCT GAAGGCCAGC TTCCCCAGAT CCTCCACTAT 
GAGCTGCTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCCGGAATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TTTGTGCTGA CAAGAATGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CACGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTCCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCTTG 
ACGTACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CCGTCTCAAT TCCAGCACCT 
GCCTACTATG CCCGCTTGGT GGCTTTCCGG GCACGATACC ACCTAGTGGA CAAAGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAGAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAAGAGCT GTGCCGCCCA 
AATCCAGAGG AAGCAGGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( XM_024989359.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845127.1 Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X2, mRNA.

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CloneID	OBa232780
Clone ID Related Accession (Same CDS sequence)	XM_024989359.1
Accession Version	XM_024989359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein argonaute-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAGG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TGCTCGACAT CAGGAACATA GATGAGCAGC CCAAAGCCCT CACGGACTCT 
CAGCGTGTGC GCTTCACCAA GGAGATCAAG GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCCAG CCATCAGACG 
TTTCCTCTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CAGTGGCCCA GTATTTCAAG 
CAGAAATATA ACCTGCAGCT CAAGTATCCC CACCTGCCTT GCCTGCAAGT TGGCCAGGAA 
CAAAAGCATA CCTACCTGCC CCTAGAGGTC TGTAACATTG TGGCTGGGCA GCGCTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG ATCGGCCCCA 
GACAGACAAG AGGAGATTAG CCGCCTGATG AAGAATGCCA GCTACAACCT AGATCCGTAC 
ATCCAGGAAT TTGGGATCAA GGTGAAGGAT GACATGACGG AGGTGACGGG ACGAGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCCACACCC 
AATCAGGGTG TCTGGGACAT GCGGGGGAAA CAGTTCTACA ATGGCATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAAAAA CAGTGTCGAG AAGAAGTGCT CAAGAACTTC 
ACGGACCAGC TGCGGAAGAT TTCCAAGGAC GCAGGGATGC CCATCCAGGG CCAGCCTTGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGT GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GGCTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTTGGAGA TACTCTGTTG GGAATGGCTA CACAGTGTGT GCAAGTGAAG 
AACGTGGTCA AGACCTCGCC TCAGACTCTG TCTAACCTTT GCCTGAAGAT CAATGTCAAA 
CTTGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCAGTCTT TCAACAACCA 
GTGATATTCC TGGGAGCAGA TGTTACACAC CCACCAGCAG GGGATGGGAA AAAACCCTCT 
ATCACAGCAG TGGTAGGCAG CATGGATGCC CACCCCAGCC GTTACTGTGC TACTGTGCGG 
GTACAGCGAC CACGGCAGGA GATCATTGAA GACTTATCCT ACATGGTACG TGAGCTGCTC 
ATCCAGTTCT ACAAGTCCAC GCGCTTCAAG CCTACCCGCA TCATCTTCTA CCGAGATGGG 
GTTCCTGAAG GCCAGCTTCC CCAGATCCTC CACTATGAGC TGCTGGCCAT TCGTGATGCC 
TGCATCAAAC TGGAAAAGGA CTACCAGCCC GGAATCACTT ATATTGTGGT GCAGAAACGC 
CATCACACCC GCCTTTTTTG TGCTGACAAG AATGAGCGAA TTGGGAAGAG TGGTAACATC 
CCAGCTGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTTGA CTTCTATCTG 
TGCAGCCACG CAGGCATCCA GGGCACCAGC CGACCATCCC ATTACTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCGGA TGAGCTCCAG ATCTTGACGT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGCTCCGT CTCAATTCCA GCACCTGCCT ACTATGCCCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT AGTGGACAAA GAGCATGACA GTGGAGAGGG GAGCCACATA 
TCGGGGCAGA GCAATGGGCG GGACCCCCAG GCCCTGGCCA AAGCCGTGCA GGTTCACCAG 
GATACTCTGC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845127.1
CDS	162..2291
Translation	MPHRVGACARGTSSLPVPARYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKALTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_024989359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus argonaute 1, RISC catalytic component (AGO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989359.1

ORF Insert Sequence:

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAGG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TGCTCGACAT CAGGAACATA GATGAGCAGC CCAAAGCCCT CACGGACTCT 
CAGCGTGTGC GCTTCACCAA GGAGATCAAG GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCCAG CCATCAGACG 
TTTCCTCTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CAGTGGCCCA GTATTTCAAG 
CAGAAATATA ACCTGCAGCT CAAGTATCCC CACCTGCCTT GCCTGCAAGT TGGCCAGGAA 
CAAAAGCATA CCTACCTGCC CCTAGAGGTC TGTAACATTG TGGCTGGGCA GCGCTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG ATCGGCCCCA 
GACAGACAAG AGGAGATTAG CCGCCTGATG AAGAATGCCA GCTACAACCT AGATCCGTAC 
ATCCAGGAAT TTGGGATCAA GGTGAAGGAT GACATGACGG AGGTGACGGG ACGAGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCCACACCC 
AATCAGGGTG TCTGGGACAT GCGGGGGAAA CAGTTCTACA ATGGCATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAAAAA CAGTGTCGAG AAGAAGTGCT CAAGAACTTC 
ACGGACCAGC TGCGGAAGAT TTCCAAGGAC GCAGGGATGC CCATCCAGGG CCAGCCTTGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGT GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GGCTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTTGGAGA TACTCTGTTG GGAATGGCTA CACAGTGTGT GCAAGTGAAG 
AACGTGGTCA AGACCTCGCC TCAGACTCTG TCTAACCTTT GCCTGAAGAT CAATGTCAAA 
CTTGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCAGTCTT TCAACAACCA 
GTGATATTCC TGGGAGCAGA TGTTACACAC CCACCAGCAG GGGATGGGAA AAAACCCTCT 
ATCACAGCAG TGGTAGGCAG CATGGATGCC CACCCCAGCC GTTACTGTGC TACTGTGCGG 
GTACAGCGAC CACGGCAGGA GATCATTGAA GACTTATCCT ACATGGTACG TGAGCTGCTC 
ATCCAGTTCT ACAAGTCCAC GCGCTTCAAG CCTACCCGCA TCATCTTCTA CCGAGATGGG 
GTTCCTGAAG GCCAGCTTCC CCAGATCCTC CACTATGAGC TGCTGGCCAT TCGTGATGCC 
TGCATCAAAC TGGAAAAGGA CTACCAGCCC GGAATCACTT ATATTGTGGT GCAGAAACGC 
CATCACACCC GCCTTTTTTG TGCTGACAAG AATGAGCGAA TTGGGAAGAG TGGTAACATC 
CCAGCTGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTTGA CTTCTATCTG 
TGCAGCCACG CAGGCATCCA GGGCACCAGC CGACCATCCC ATTACTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCGGA TGAGCTCCAG ATCTTGACGT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGCTCCGT CTCAATTCCA GCACCTGCCT ACTATGCCCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT AGTGGACAAA GAGCATGACA GTGGAGAGGG GAGCCACATA 
TCGGGGCAGA GCAATGGGCG GGACCCCCAG GCCCTGGCCA AAGCCGTGCA GGTTCACCAG 
GATACTCTGC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

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AGO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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