Ago1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ago1
Entrez Gene ID	236511
Full Name	argonaute RISC catalytic subunit 1
Synonyms	Eif2c1
General protein information	Preferred Names argonaute RISC catalytic subunit 1 Names protein argonaute-1 Piwi/Argonaute family protein meIF2C1 argonaute 1 argonaute RISC catalytic component 1 argonaute1 eIF-2C 1 eIF2C 1 eukaryotic translation initiation factor 2C, 1 mAgo1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2
Summary	This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153403.2	NP_700452.2	protein argonaute-1
NM_001317173.1	NP_001304102.1	protein argonaute-1 isoform 1x
NM_001317174.1	NP_001304103.1	protein argonaute-1 isoform 2
XM_011240521.1	XP_011238823.1	protein argonaute-1 isoform X2
NM_153403.3	NP_700452.2	protein argonaute-1 isoform 1
XM_011240520.2	XP_011238822.1	protein argonaute-1 isoform X1
XM_011240521.2	XP_011238823.1	protein argonaute-1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570775	XP_005158529.1
Gallus gallus (chicken)	EIF2C1	XP_425781.3
Xenopus tropicalis (tropical clawed frog)	ago4	XP_002936260.1
Homo sapiens (human)	AGO1	NP_036331.1
Canis lupus familiaris (dog)	AGO1	XP_532563.3
Mus musculus (house mouse)	Ago1	NP_700452.2
Rattus norvegicus (Norway rat)	Ago1	NP_001178694.1
Bos taurus (cattle)	EIF2C1	NP_001192828.1
Pan troglodytes (chimpanzee)	EIF2C1	XP_513312.2

Gene Ontology
Bibliography

Related articles in PubMed

Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells.
Zheng Y, Guo X, He W, Shao Z, Zhang X, Yang J, Shen Y, Luo X, Cao J
International immunopharmacology34259-62(2016 May)

MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production.
Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM
Immunity44(4)821-32(2016 Apr)

Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.
Sun S, Ma J, Zhang Q, Wang Q, Zhou L, Bai F, Hu H, Chang P, Yu J, Gao B
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology25(2)120-6(2016 Mar-Apr)

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR-71
Title: Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells.

Results from the liver show that, siRNA targets 3'UTR and the coding sequence (CDs) of endogenous genes in the presence Ago2 but in its absence, only 3'UTR-targeted siRNA-mediated knockdown are active with the help of Ago1 and Ago3.
Title: The roles of individual mammalian argonautes in RNA interference in vivo.

Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression
Title: Quantitative functions of Argonaute proteins in mammalian development.

enhanced flu susceptibility of Ago1/3 double-knockout mice arises from an intrinsic impairment in the ability of lung cells to tolerate flu-elicited inflammation.
Title: Enhanced susceptibility of Ago1/3 double-null mice to influenza A virus infection.

describes cloning rat sequence and used RNA interference to show that the GERp95 orthologue in C. elegans is important for maturation of germ-line stem cells in the gonad.
Title: GERp95, a membrane-associated protein that belongs to a family of proteins involved in stem cell differentiation.

The following Ago1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ago1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33571	NM_001317174.1 Latest version!	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu73856	XM_011240520.2 Latest version!	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu18341	NM_153403.3 Latest version!	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu76891	NM_001317173.1 Latest version!	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu73857	XM_011240521.2 Latest version!	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu18341	NM_153403.2	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu73857	XM_011240521.1	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ago1 ( NM_001317174.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001304103.1 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 2, mRNA.

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CloneID	OMu33571
Clone ID Related Accession (Same CDS sequence)	NM_001317174.1
Accession Version	NM_001317174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ130124.1, AK080954.1, CV559803.1, AL606976.25 and AI451331.1. On Nov 19, 2015 this sequence version replaced XM_006503067.1. Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 5' coding region and initiates translation from an alternate start codon compared to variant 1. The resulting isoform (2) is shorter with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK080954.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG AACCGGGCCA TTGCTACGCC CAACCAAGGT 
GTCTGGGACA TGCGCGGGAA ACAGTTCTAC AATGGGATTG AGATCAAAGT CTGGGCCATC 
GCCTGCTTCG CACCCCAGAA ACAGTGTCGA GAAGAGGTGC TCAAGAACTT CACAGACCAG 
CTTCGGAAGA TTTCCAAGGA TGCAGGGATG CCCATCCAGG GTCAGCCATG CTTCTGCAAA 
TACGCACAGG GGGCAGACAG CGTGGAGCCC ATGTTCCGGC ATCTCAAGAA CACCTACTCA 
GGACTGCAGC TCATTATCGT CATCCTGCCC GGGAAGACGC CAGTGTATGC TGAAGTGAAA 
CGTGTCGGAG ATACACTCTT GGGAATGGCA ACACAGTGTG TGCAGGTGAA GAATGTGGTC 
AAGACGTCAC CGCAGACTCT GTCCAACCTC TGCCTCAAGA TCAATGTCAA ACTCGGTGGC 
ATTAACAACA TCCTAGTCCC ACACCAGCGC TCTGCTGTTT TTCAACAGCC AGTGATTTTC 
CTGGGAGCAG ATGTTACACA TCCCCCAGCT GGGGATGGGA AGAAACCATC TATCACAGCA 
GTGGTAGGCA GCATGGACGC ACACCCCAGC CGATACTGTG CCACCGTGCG TGTGCAGCGC 
CCACGGCAGG AGATCATTGA AGACTTGTCC TATATGGTGC GCGAGCTGCT TATCCAGTTC 
TACAAGTCCA CCCGCTTCAA GCCCACCCGC ATCATCTTCT ACCGCGACGG CGTCCCCGAG 
GGCCAGCTGC CCCAGATTCT TCACTATGAG CTGCTTGCCA TTCGAGATGC ATGCATCAAA 
CTGGAAAAAG ACTACCAGCC TGGGATCACA TATATTGTGG TGCAGAAGCG CCATCACACC 
CGCCTGTTCT GTGCTGACAA GAATGAGCGG ATTGGGAAGA GTGGTAACAT CCCAGCAGGG 
ACCACTGTGG ACACCAACAT CACCCACCCA TTTGAGTTCG ACTTCTATCT GTGCAGCCAT 
GCAGGCATCC AGGGTACCAG CCGACCATCC CATTATTATG TCCTTTGGGA TGACAACCGT 
TTCACAGCAG ATGAACTCCA GATCTTGACA TACCAGCTGT GCCACACTTA CGTACGATGC 
ACACGTTCTG TCTCTATCCC AGCACCTGCC TACTATGCTC GCTTGGTGGC TTTCCGGGCA 
CGATACCACC TGGTGGACAA GGAACATGAC AGTGGAGAGG GGAGCCACAT ATCAGGGCAA 
AGCAATGGGC GAGACCCCCA GGCCCTGGCC AAAGCTGTGC AGGTTCACCA GGATACTCTA 
CGCACCATGT ACTTCGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304103.1
CDS	698..2818
Translation	MPHRVGACARGTSSLPVPARYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_001317174.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317174.1

ORF Insert Sequence:

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG AACCGGGCCA TTGCTACGCC CAACCAAGGT 
GTCTGGGACA TGCGCGGGAA ACAGTTCTAC AATGGGATTG AGATCAAAGT CTGGGCCATC 
GCCTGCTTCG CACCCCAGAA ACAGTGTCGA GAAGAGGTGC TCAAGAACTT CACAGACCAG 
CTTCGGAAGA TTTCCAAGGA TGCAGGGATG CCCATCCAGG GTCAGCCATG CTTCTGCAAA 
TACGCACAGG GGGCAGACAG CGTGGAGCCC ATGTTCCGGC ATCTCAAGAA CACCTACTCA 
GGACTGCAGC TCATTATCGT CATCCTGCCC GGGAAGACGC CAGTGTATGC TGAAGTGAAA 
CGTGTCGGAG ATACACTCTT GGGAATGGCA ACACAGTGTG TGCAGGTGAA GAATGTGGTC 
AAGACGTCAC CGCAGACTCT GTCCAACCTC TGCCTCAAGA TCAATGTCAA ACTCGGTGGC 
ATTAACAACA TCCTAGTCCC ACACCAGCGC TCTGCTGTTT TTCAACAGCC AGTGATTTTC 
CTGGGAGCAG ATGTTACACA TCCCCCAGCT GGGGATGGGA AGAAACCATC TATCACAGCA 
GTGGTAGGCA GCATGGACGC ACACCCCAGC CGATACTGTG CCACCGTGCG TGTGCAGCGC 
CCACGGCAGG AGATCATTGA AGACTTGTCC TATATGGTGC GCGAGCTGCT TATCCAGTTC 
TACAAGTCCA CCCGCTTCAA GCCCACCCGC ATCATCTTCT ACCGCGACGG CGTCCCCGAG 
GGCCAGCTGC CCCAGATTCT TCACTATGAG CTGCTTGCCA TTCGAGATGC ATGCATCAAA 
CTGGAAAAAG ACTACCAGCC TGGGATCACA TATATTGTGG TGCAGAAGCG CCATCACACC 
CGCCTGTTCT GTGCTGACAA GAATGAGCGG ATTGGGAAGA GTGGTAACAT CCCAGCAGGG 
ACCACTGTGG ACACCAACAT CACCCACCCA TTTGAGTTCG ACTTCTATCT GTGCAGCCAT 
GCAGGCATCC AGGGTACCAG CCGACCATCC CATTATTATG TCCTTTGGGA TGACAACCGT 
TTCACAGCAG ATGAACTCCA GATCTTGACA TACCAGCTGT GCCACACTTA CGTACGATGC 
ACACGTTCTG TCTCTATCCC AGCACCTGCC TACTATGCTC GCTTGGTGGC TTTCCGGGCA 
CGATACCACC TGGTGGACAA GGAACATGAC AGTGGAGAGG GGAGCCACAT ATCAGGGCAA 
AGCAATGGGC GAGACCCCCA GGCCCTGGCC AAAGCTGTGC AGGTTCACCA GGATACTCTA 
CGCACCATGT ACTTCGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( XM_011240520.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238822.1 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73856
Clone ID Related Accession (Same CDS sequence)	XM_011240520.2
Accession Version	XM_011240520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCTACG CCCAACCAAG GTGTCTGGGA CATGCGCGGG 
AAACAGTTCT ACAATGGGAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAG 
AAACAGTGTC GAGAAGAGGT GCTCAAGAAC TTCACAGACC AGCTTCGGAA GATTTCCAAG 
GATGCAGGGA TGCCCATCCA GGGTCAGCCA TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGCGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGACTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTCGG AGATACACTC 
TTGGGAATGG CAACACAGTG TGTGCAGGTG AAGAATGTGG TCAAGACGTC ACCGCAGACT 
CTGTCCAACC TCTGCCTCAA GATCAATGTC AAACTCGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCTGT TTTTCAACAG CCAGTGATTT TCCTGGGAGC AGATGTTACA 
CATCCCCCAG CTGGGGATGG GAAGAAACCA TCTATCACAG CAGTGGTAGG CAGCATGGAC 
GCACACCCCA GCCGATACTG TGCCACCGTG CGTGTGCAGC GCCCACGGCA GGAGATCATT 
GAAGACTTGT CCTATATGGT GCGCGAGCTG CTTATCCAGT TCTACAAGTC CACCCGCTTC 
AAGCCCACCC GCATCATCTT CTACCGCGAC GGCGTCCCCG AGGGCCAGCT GCCCCAGATT 
CTTCACTATG AGCTGCTTGC CATTCGAGAT GCATGCATCA AACTGGAAAA AGACTACCAG 
CCTGGGATCA CATATATTGT GGTGCAGAAG CGCCATCACA CCCGCCTGTT CTGTGCTGAC 
AAGAATGAGC GGATTGGGAA GAGTGGTAAC ATCCCAGCAG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT CGACTTCTAT CTGTGCAGCC ATGCAGGCAT CCAGGGTACC 
AGCCGACCAT CCCATTATTA TGTCCTTTGG GATGACAACC GTTTCACAGC AGATGAACTC 
CAGATCTTGA CATACCAGCT GTGCCACACT TACGTACGAT GCACACGTTC TGTCTCTATC 
CCAGCACCTG CCTACTATGC TCGCTTGGTG GCTTTCCGGG CACGATACCA CCTGGTGGAC 
AAGGAACATG ACAGTGGAGA GGGGAGCCAC ATATCAGGGC AAAGCAATGG GCGAGACCCC 
CAGGCCCTGG CCAAAGCTGT GCAGGTTCAC CAGGATACTC TACGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238822.1
CDS	289..2871
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_011240520.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240520.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCTACG CCCAACCAAG GTGTCTGGGA CATGCGCGGG 
AAACAGTTCT ACAATGGGAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAG 
AAACAGTGTC GAGAAGAGGT GCTCAAGAAC TTCACAGACC AGCTTCGGAA GATTTCCAAG 
GATGCAGGGA TGCCCATCCA GGGTCAGCCA TGCTTCTGCA AATACGCACA GGGGGCAGAC 
AGCGTGGAGC CCATGTTCCG GCATCTCAAG AACACCTACT CAGGACTGCA GCTCATTATC 
GTCATCCTGC CCGGGAAGAC GCCAGTGTAT GCTGAAGTGA AACGTGTCGG AGATACACTC 
TTGGGAATGG CAACACAGTG TGTGCAGGTG AAGAATGTGG TCAAGACGTC ACCGCAGACT 
CTGTCCAACC TCTGCCTCAA GATCAATGTC AAACTCGGTG GCATTAACAA CATCCTAGTC 
CCACACCAGC GCTCTGCTGT TTTTCAACAG CCAGTGATTT TCCTGGGAGC AGATGTTACA 
CATCCCCCAG CTGGGGATGG GAAGAAACCA TCTATCACAG CAGTGGTAGG CAGCATGGAC 
GCACACCCCA GCCGATACTG TGCCACCGTG CGTGTGCAGC GCCCACGGCA GGAGATCATT 
GAAGACTTGT CCTATATGGT GCGCGAGCTG CTTATCCAGT TCTACAAGTC CACCCGCTTC 
AAGCCCACCC GCATCATCTT CTACCGCGAC GGCGTCCCCG AGGGCCAGCT GCCCCAGATT 
CTTCACTATG AGCTGCTTGC CATTCGAGAT GCATGCATCA AACTGGAAAA AGACTACCAG 
CCTGGGATCA CATATATTGT GGTGCAGAAG CGCCATCACA CCCGCCTGTT CTGTGCTGAC 
AAGAATGAGC GGATTGGGAA GAGTGGTAAC ATCCCAGCAG GGACCACTGT GGACACCAAC 
ATCACCCACC CATTTGAGTT CGACTTCTAT CTGTGCAGCC ATGCAGGCAT CCAGGGTACC 
AGCCGACCAT CCCATTATTA TGTCCTTTGG GATGACAACC GTTTCACAGC AGATGAACTC 
CAGATCTTGA CATACCAGCT GTGCCACACT TACGTACGAT GCACACGTTC TGTCTCTATC 
CCAGCACCTG CCTACTATGC TCGCTTGGTG GCTTTCCGGG CACGATACCA CCTGGTGGAC 
AAGGAACATG ACAGTGGAGA GGGGAGCCAC ATATCAGGGC AAAGCAATGG GCGAGACCCC 
CAGGCCCTGG CCAAAGCTGT GCAGGTTCAC CAGGATACTC TACGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( XM_011240521.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238823.1 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.

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CloneID	OMu73857
Clone ID Related Accession (Same CDS sequence)	XM_011240521.1 , XM_011240521.2
Accession Version	XM_011240521.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCTACGCCC 
AACCAAGGTG TCTGGGACAT GCGCGGGAAA CAGTTCTACA ATGGGATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAGAAA CAGTGTCGAG AAGAGGTGCT CAAGAACTTC 
ACAGACCAGC TTCGGAAGAT TTCCAAGGAT GCAGGGATGC CCATCCAGGG TCAGCCATGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGC GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GACTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTCGGAGA TACACTCTTG GGAATGGCAA CACAGTGTGT GCAGGTGAAG 
AATGTGGTCA AGACGTCACC GCAGACTCTG TCCAACCTCT GCCTCAAGAT CAATGTCAAA 
CTCGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCTGTTTT TCAACAGCCA 
GTGATTTTCC TGGGAGCAGA TGTTACACAT CCCCCAGCTG GGGATGGGAA GAAACCATCT 
ATCACAGCAG TGGTAGGCAG CATGGACGCA CACCCCAGCC GATACTGTGC CACCGTGCGT 
GTGCAGCGCC CACGGCAGGA GATCATTGAA GACTTGTCCT ATATGGTGCG CGAGCTGCTT 
ATCCAGTTCT ACAAGTCCAC CCGCTTCAAG CCCACCCGCA TCATCTTCTA CCGCGACGGC 
GTCCCCGAGG GCCAGCTGCC CCAGATTCTT CACTATGAGC TGCTTGCCAT TCGAGATGCA 
TGCATCAAAC TGGAAAAAGA CTACCAGCCT GGGATCACAT ATATTGTGGT GCAGAAGCGC 
CATCACACCC GCCTGTTCTG TGCTGACAAG AATGAGCGGA TTGGGAAGAG TGGTAACATC 
CCAGCAGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTCGA CTTCTATCTG 
TGCAGCCATG CAGGCATCCA GGGTACCAGC CGACCATCCC ATTATTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCAGA TGAACTCCAG ATCTTGACAT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGTTCTGT CTCTATCCCA GCACCTGCCT ACTATGCTCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT GGTGGACAAG GAACATGACA GTGGAGAGGG GAGCCACATA 
TCAGGGCAAA GCAATGGGCG AGACCCCCAG GCCCTGGCCA AAGCTGTGCA GGTTCACCAG 
GATACTCTAC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238823.1
CDS	301..2430
Translation	MPHRVGACARGTSSLPVPARYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_011240521.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240521.1

ORF Insert Sequence:

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCTACGCCC 
AACCAAGGTG TCTGGGACAT GCGCGGGAAA CAGTTCTACA ATGGGATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAGAAA CAGTGTCGAG AAGAGGTGCT CAAGAACTTC 
ACAGACCAGC TTCGGAAGAT TTCCAAGGAT GCAGGGATGC CCATCCAGGG TCAGCCATGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGC GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GACTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTCGGAGA TACACTCTTG GGAATGGCAA CACAGTGTGT GCAGGTGAAG 
AATGTGGTCA AGACGTCACC GCAGACTCTG TCCAACCTCT GCCTCAAGAT CAATGTCAAA 
CTCGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCTGTTTT TCAACAGCCA 
GTGATTTTCC TGGGAGCAGA TGTTACACAT CCCCCAGCTG GGGATGGGAA GAAACCATCT 
ATCACAGCAG TGGTAGGCAG CATGGACGCA CACCCCAGCC GATACTGTGC CACCGTGCGT 
GTGCAGCGCC CACGGCAGGA GATCATTGAA GACTTGTCCT ATATGGTGCG CGAGCTGCTT 
ATCCAGTTCT ACAAGTCCAC CCGCTTCAAG CCCACCCGCA TCATCTTCTA CCGCGACGGC 
GTCCCCGAGG GCCAGCTGCC CCAGATTCTT CACTATGAGC TGCTTGCCAT TCGAGATGCA 
TGCATCAAAC TGGAAAAAGA CTACCAGCCT GGGATCACAT ATATTGTGGT GCAGAAGCGC 
CATCACACCC GCCTGTTCTG TGCTGACAAG AATGAGCGGA TTGGGAAGAG TGGTAACATC 
CCAGCAGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTCGA CTTCTATCTG 
TGCAGCCATG CAGGCATCCA GGGTACCAGC CGACCATCCC ATTATTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCAGA TGAACTCCAG ATCTTGACAT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGTTCTGT CTCTATCCCA GCACCTGCCT ACTATGCTCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT GGTGGACAAG GAACATGACA GTGGAGAGGG GAGCCACATA 
TCAGGGCAAA GCAATGGGCG AGACCCCCAG GCCCTGGCCA AAGCTGTGCA GGTTCACCAG 
GATACTCTAC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( NM_153403.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_700452.2 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), mRNA.

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CloneID	OMu18341
Clone ID Related Accession (Same CDS sequence)	NM_153403.2 , NM_153403.3
Accession Version	NM_153403.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	protein argonaute-1
Comment

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_700452.2

Translation

MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_153403.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153403.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( NM_153403.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_700452.2 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.

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CloneID	OMu18341
Clone ID Related Accession (Same CDS sequence)	NM_153403.2 , NM_153403.3
Accession Version	NM_153403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ130124.1, BC129914.1, AK080954.1, CV559803.1, AL606976.25 and AI451331.1. On Nov 19, 2015 this sequence version replaced NM_153403.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC129914.1, AB081471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_700452.2
CDS	163..2736
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_153403.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153403.3

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( NM_001317173.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001304102.1 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu76891
Clone ID Related Accession (Same CDS sequence)	NM_001317173.1
Accession Version	NM_001317173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ130124.1, BC129914.1, AK080954.1, CV559803.1, AL606976.25 and AI451331.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC129914.1, AB081471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCTGGGAGCT GTACCACCCA 
AATCCAGAGG AAGCAAGGCA GAGGGAGGTG GGGTCGGAAG GAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304102.1
CDS	163..2850
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*RQNAVTSLDRRKLSKPWELYHPNPEEARQREVGSEGV

Target ORF information:

RefSeq Version	NM_001317173.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317173.1

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCGGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTTCAGGCAC CCCGCCGGCC TGGCATTGGC ACTGTGGGCA AACCAATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTTTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AATACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA TATTTACACG 
GTCACAGCAC TGCCCATTGG CAATGAGAGG GTTGACTTTG AGGTGACAAT CCCCGGGGAG 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCC TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGACGAAC AACCCAAGCC CCTCACGGAT TCCCAGCGTG TTCGGTTTAC CAAGGAGATA 
AAAGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GCCGCCCTGC TAGCCATCAG ACGTTCCCCT TGCAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACTGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTACA AGTTGGGCAA GAACAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGCCGCCTG 
ATGAAGAATG CTAGCTACAA CCTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGACGTGTG CTGCCGGCGC CCATCTTGCA GTATGGCGGC 
CGGAACCGGG CCATTGCTAC GCCCAACCAA GGTGTCTGGG ACATGCGCGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA GAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTTCGGA AGATTTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGCTTCTGC AAATACGCAC AGGGGGCAGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAACACCTAC TCAGGACTGC AGCTCATTAT CGTCATCCTG 
CCCGGGAAGA CGCCAGTGTA TGCTGAAGTG AAACGTGTCG GAGATACACT CTTGGGAATG 
GCAACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACGT CACCGCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCTG TTTTTCAACA GCCAGTGATT TTCCTGGGAG CAGATGTTAC ACATCCCCCA 
GCTGGGGATG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGCATGGA CGCACACCCC 
AGCCGATACT GTGCCACCGT GCGTGTGCAG CGCCCACGGC AGGAGATCAT TGAAGACTTG 
TCCTATATGG TGCGCGAGCT GCTTATCCAG TTCTACAAGT CCACCCGCTT CAAGCCCACC 
CGCATCATCT TCTACCGCGA CGGCGTCCCC GAGGGCCAGC TGCCCCAGAT TCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCATGCATC AAACTGGAAA AAGACTACCA GCCTGGGATC 
ACATATATTG TGGTGCAGAA GCGCCATCAC ACCCGCCTGT TCTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCA GGGACCACTG TGGACACCAA CATCACCCAC 
CCATTTGAGT TCGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGTAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAACT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CTCGCTTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAACAT 
GACAGTGGAG AGGGGAGCCA CATATCAGGG CAAAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCTG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCTGGGAGCT GTACCACCCA 
AATCCAGAGG AAGCAAGGCA GAGGGAGGTG GGGTCGGAAG GAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( XM_011240521.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238823.1 Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.

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CloneID	OMu73857
Clone ID Related Accession (Same CDS sequence)	XM_011240521.1 , XM_011240521.2
Accession Version	XM_011240521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein argonaute-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCTACGCCC 
AACCAAGGTG TCTGGGACAT GCGCGGGAAA CAGTTCTACA ATGGGATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAGAAA CAGTGTCGAG AAGAGGTGCT CAAGAACTTC 
ACAGACCAGC TTCGGAAGAT TTCCAAGGAT GCAGGGATGC CCATCCAGGG TCAGCCATGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGC GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GACTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTCGGAGA TACACTCTTG GGAATGGCAA CACAGTGTGT GCAGGTGAAG 
AATGTGGTCA AGACGTCACC GCAGACTCTG TCCAACCTCT GCCTCAAGAT CAATGTCAAA 
CTCGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCTGTTTT TCAACAGCCA 
GTGATTTTCC TGGGAGCAGA TGTTACACAT CCCCCAGCTG GGGATGGGAA GAAACCATCT 
ATCACAGCAG TGGTAGGCAG CATGGACGCA CACCCCAGCC GATACTGTGC CACCGTGCGT 
GTGCAGCGCC CACGGCAGGA GATCATTGAA GACTTGTCCT ATATGGTGCG CGAGCTGCTT 
ATCCAGTTCT ACAAGTCCAC CCGCTTCAAG CCCACCCGCA TCATCTTCTA CCGCGACGGC 
GTCCCCGAGG GCCAGCTGCC CCAGATTCTT CACTATGAGC TGCTTGCCAT TCGAGATGCA 
TGCATCAAAC TGGAAAAAGA CTACCAGCCT GGGATCACAT ATATTGTGGT GCAGAAGCGC 
CATCACACCC GCCTGTTCTG TGCTGACAAG AATGAGCGGA TTGGGAAGAG TGGTAACATC 
CCAGCAGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTCGA CTTCTATCTG 
TGCAGCCATG CAGGCATCCA GGGTACCAGC CGACCATCCC ATTATTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCAGA TGAACTCCAG ATCTTGACAT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGTTCTGT CTCTATCCCA GCACCTGCCT ACTATGCTCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT GGTGGACAAG GAACATGACA GTGGAGAGGG GAGCCACATA 
TCAGGGCAAA GCAATGGGCG AGACCCCCAG GCCCTGGCCA AAGCTGTGCA GGTTCACCAG 
GATACTCTAC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238823.1
CDS	822..2951
Translation	MPHRVGACARGTSSLPVPARYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_011240521.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240521.2

ORF Insert Sequence:

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GACGAACAAC CCAAGCCCCT CACGGATTCC 
CAGCGTGTTC GGTTTACCAA GGAGATAAAA GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGCC GCCCTGCTAG CCATCAGACG 
TTCCCCTTGC AGCTGGAGAG TGGACAGACT GTGGAGTGCA CTGTGGCACA GTATTTCAAG 
CAGAAATATA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTACAAGT TGGGCAAGAA 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG CCGCCTGATG AAGAATGCTA GCTACAACCT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACAGG ACGTGTGCTG 
CCGGCGCCCA TCTTGCAGTA TGGCGGCCGG GTGAGCAGGA ACCGGGCCAT TGCTACGCCC 
AACCAAGGTG TCTGGGACAT GCGCGGGAAA CAGTTCTACA ATGGGATTGA GATCAAAGTC 
TGGGCCATCG CCTGCTTCGC ACCCCAGAAA CAGTGTCGAG AAGAGGTGCT CAAGAACTTC 
ACAGACCAGC TTCGGAAGAT TTCCAAGGAT GCAGGGATGC CCATCCAGGG TCAGCCATGC 
TTCTGCAAAT ACGCACAGGG GGCAGACAGC GTGGAGCCCA TGTTCCGGCA TCTCAAGAAC 
ACCTACTCAG GACTGCAGCT CATTATCGTC ATCCTGCCCG GGAAGACGCC AGTGTATGCT 
GAAGTGAAAC GTGTCGGAGA TACACTCTTG GGAATGGCAA CACAGTGTGT GCAGGTGAAG 
AATGTGGTCA AGACGTCACC GCAGACTCTG TCCAACCTCT GCCTCAAGAT CAATGTCAAA 
CTCGGTGGCA TTAACAACAT CCTAGTCCCA CACCAGCGCT CTGCTGTTTT TCAACAGCCA 
GTGATTTTCC TGGGAGCAGA TGTTACACAT CCCCCAGCTG GGGATGGGAA GAAACCATCT 
ATCACAGCAG TGGTAGGCAG CATGGACGCA CACCCCAGCC GATACTGTGC CACCGTGCGT 
GTGCAGCGCC CACGGCAGGA GATCATTGAA GACTTGTCCT ATATGGTGCG CGAGCTGCTT 
ATCCAGTTCT ACAAGTCCAC CCGCTTCAAG CCCACCCGCA TCATCTTCTA CCGCGACGGC 
GTCCCCGAGG GCCAGCTGCC CCAGATTCTT CACTATGAGC TGCTTGCCAT TCGAGATGCA 
TGCATCAAAC TGGAAAAAGA CTACCAGCCT GGGATCACAT ATATTGTGGT GCAGAAGCGC 
CATCACACCC GCCTGTTCTG TGCTGACAAG AATGAGCGGA TTGGGAAGAG TGGTAACATC 
CCAGCAGGGA CCACTGTGGA CACCAACATC ACCCACCCAT TTGAGTTCGA CTTCTATCTG 
TGCAGCCATG CAGGCATCCA GGGTACCAGC CGACCATCCC ATTATTATGT CCTTTGGGAT 
GACAACCGTT TCACAGCAGA TGAACTCCAG ATCTTGACAT ACCAGCTGTG CCACACTTAC 
GTACGATGCA CACGTTCTGT CTCTATCCCA GCACCTGCCT ACTATGCTCG CTTGGTGGCT 
TTCCGGGCAC GATACCACCT GGTGGACAAG GAACATGACA GTGGAGAGGG GAGCCACATA 
TCAGGGCAAA GCAATGGGCG AGACCCCCAG GCCCTGGCCA AAGCTGTGCA GGTTCACCAG 
GATACTCTAC GCACCATGTA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells.
International immunopharmacology34259-62(2016 May)
Zheng Y,Guo X,He W,Shao Z,Zhang X,Yang J,Shen Y,Luo X,Cao J

MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production.
Immunity44(4)821-32(2016 Apr)
Pua HH,Steiner DF,Patel S,Gonzalez JR,Ortiz-Carpena JF,Kageyama R,Chiou NT,Gallman A,de Kouchkovsky D,Jeker LT,McManus MT,Erle DJ,Ansel KM

Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology25(2)120-6(2016 Mar-Apr)
Sun S,Ma J,Zhang Q,Wang Q,Zhou L,Bai F,Hu H,Chang P,Yu J,Gao B

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

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Ago1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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