Ago1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ago1
Entrez Gene ID	313594
Full Name	argonaute 1, RISC catalytic component
Synonyms	Eif2c1
General protein information	Preferred Names argonaute 1, RISC catalytic component Names protein argonaute-1 argonaute RISC catalytic component 1 eukaryotic translation initiation factor 2C, 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191765.2	NP_001178694.1	protein argonaute-1 isoform 1
XM_008764025.2	XP_008762247.1	protein argonaute-1 isoform X1
XM_017593401.1	XP_017448890.1	protein argonaute-1 isoform X2
NM_001317181.1	NP_001304110.1	protein argonaute-1 isoform 1x

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570775	XP_005158529.1
Gallus gallus (chicken)	EIF2C1	XP_425781.3
Xenopus tropicalis (tropical clawed frog)	ago4	XP_002936260.1
Homo sapiens (human)	AGO1	NP_036331.1
Canis lupus familiaris (dog)	AGO1	XP_532563.3
Mus musculus (house mouse)	Ago1	NP_700452.2
Rattus norvegicus (Norway rat)	Ago1	NP_001178694.1
Bos taurus (cattle)	EIF2C1	NP_001192828.1
Pan troglodytes (chimpanzee)	EIF2C1	XP_513312.2

Gene Ontology
Bibliography

Related articles in PubMed

Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.
Sun S, Ma J, Zhang Q, Wang Q, Zhou L, Bai F, Hu H, Chang P, Yu J, Gao B
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology25(2)120-6(2016 Mar-Apr)

Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA.
Ghosh U, Adhya S
The Journal of biological chemistry291(13)7119-27(2016 Mar)

Cellular microRNAs up-regulate transcription via interaction with promoter TATA-box motifs.
Zhang Y, Fan M, Zhang X, Huang F, Wu K, Zhang J, Liu J, Huang Z, Luo H, Tao L, Zhang H
RNA (New York, N.Y.)20(12)1878-89(2014 Dec)

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AGO1 and AGO2 play non-equivalent roles of in mRNA turnover and translation of Ccnd1 mRNA.
Title: Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA.

The following Ago1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ago1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa34921	NM_001191765.2 Latest version!	Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47244	XM_008764025.2 Latest version!	Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa63211	NM_001317181.1 Latest version!	Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa61237	XM_017593401.1 Latest version!	Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ago1 ( NM_001191765.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178694.1 Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa34921
Clone ID Related Accession (Same CDS sequence)	NM_001191765.2
Accession Version	NM_001191765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-01
Organism	Rattus norvegicus(Norway rat)
Product	protein argonaute-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125765.5 and FM038730.1. On Sep 18, 2014 this sequence version replaced NM_001191765.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCTAC ACCCAACCAA GGTGTCTGGG ACATGCGTGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAATGT 
CGAGAAGAGG TGCTCAAGAA CTTCACGGAC CAGCTTCGGA AGATCTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGTTTCTGC AAATACGCAC AGGGGGCGGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAATACCTAC TCAGGACTGC AGCTCATTAT TGTCATCCTG 
CCCGGGAAGA CGCCGGTGTA TGCTGAAGTG AAGCGTGTTG GAGATACGCT CTTGGGAATG 
GCGACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATCAACA ACATCCTTGT CCCACACCAG 
CGCTCTGCTG TTTTCCAACA GCCAGTGATT TTTCTGGGAG CAGACGTTAC ACACCCCCCG 
GCTGGGGACG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGTATGGA TGCACACCCC 
AGCCGATACT GTGCCACTGT GCGTGTACAG CGTCCACGGC AGGAGATCAT TGAGGACTTG 
TCCTATATGG TGCGGGAGCT GCTCATCCAG TTCTACAAGT CCACCCGATT CAAGCCCACC 
CGAATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TGCCTCAGAT CCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCCTGCATC AAACTGGAAA GAGACTACCA GCCTGGGATC 
ACGTACATCG TGGTACAGAA GCGACATCAC ACCCGCCTCT TTTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACTCAC 
CCGTTTGAGT TTGACTTCTA CCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAGCT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTATGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCCCGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCTGGG CAGAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178694.1
CDS	204..2777
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLERDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_001191765.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191765.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCTAC ACCCAACCAA GGTGTCTGGG ACATGCGTGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAATGT 
CGAGAAGAGG TGCTCAAGAA CTTCACGGAC CAGCTTCGGA AGATCTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGTTTCTGC AAATACGCAC AGGGGGCGGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAATACCTAC TCAGGACTGC AGCTCATTAT TGTCATCCTG 
CCCGGGAAGA CGCCGGTGTA TGCTGAAGTG AAGCGTGTTG GAGATACGCT CTTGGGAATG 
GCGACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATCAACA ACATCCTTGT CCCACACCAG 
CGCTCTGCTG TTTTCCAACA GCCAGTGATT TTTCTGGGAG CAGACGTTAC ACACCCCCCG 
GCTGGGGACG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGTATGGA TGCACACCCC 
AGCCGATACT GTGCCACTGT GCGTGTACAG CGTCCACGGC AGGAGATCAT TGAGGACTTG 
TCCTATATGG TGCGGGAGCT GCTCATCCAG TTCTACAAGT CCACCCGATT CAAGCCCACC 
CGAATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TGCCTCAGAT CCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCCTGCATC AAACTGGAAA GAGACTACCA GCCTGGGATC 
ACGTACATCG TGGTACAGAA GCGACATCAC ACCCGCCTCT TTTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACTCAC 
CCGTTTGAGT TTGACTTCTA CCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAGCT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTATGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCCCGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCTGGG CAGAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( XM_008764025.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762247.1 Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47244
Clone ID Related Accession (Same CDS sequence)	XM_008764025.2
Accession Version	XM_008764025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein argonaute-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCTACA CCCAACCAAG GTGTCTGGGA CATGCGTGGG 
AAACAGTTCT ACAATGGGAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAATGTC GAGAAGAGGT GCTCAAGAAC TTCACGGACC AGCTTCGGAA GATCTCCAAG 
GATGCAGGGA TGCCCATCCA GGGTCAGCCA TGTTTCTGCA AATACGCACA GGGGGCGGAC 
AGCGTGGAGC CCATGTTCCG GCATCTCAAG AATACCTACT CAGGACTGCA GCTCATTATT 
GTCATCCTGC CCGGGAAGAC GCCGGTGTAT GCTGAAGTGA AGCGTGTTGG AGATACGCTC 
TTGGGAATGG CGACACAGTG TGTGCAGGTG AAGAATGTGG TCAAGACCTC ACCTCAGACT 
CTGTCCAACC TCTGCCTCAA GATCAATGTC AAACTCGGTG GCATCAACAA CATCCTTGTC 
CCACACCAGC GCTCTGCTGT TTTCCAACAG CCAGTGATTT TTCTGGGAGC AGACGTTACA 
CACCCCCCGG CTGGGGACGG GAAGAAACCA TCTATCACAG CAGTGGTAGG CAGTATGGAT 
GCACACCCCA GCCGATACTG TGCCACTGTG CGTGTACAGC GTCCACGGCA GGAGATCATT 
GAGGACTTGT CCTATATGGT GCGGGAGCTG CTCATCCAGT TCTACAAGTC CACCCGATTC 
AAGCCCACCC GAATCATCTT CTACCGAGAT GGGGTCCCTG AAGGCCAGCT GCCTCAGATC 
CTTCACTATG AGCTGCTTGC CATTCGAGAT GCCTGCATCA AACTGGAAAG AGACTACCAG 
CCTGGGATCA CGTACATCGT GGTACAGAAG CGACATCACA CCCGCCTCTT TTGTGCTGAC 
AAGAATGAGC GGATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACTCACC CGTTTGAGTT TGACTTCTAC CTGTGCAGCC ATGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTATTA TGTCCTTTGG GATGACAACC GTTTCACAGC AGATGAGCTC 
CAGATCTTGA CATACCAGCT GTGCCACACT TATGTACGAT GCACACGTTC TGTCTCTATC 
CCAGCACCTG CCTACTATGC CCGCCTGGTG GCTTTCCGGG CCCGATACCA CCTGGTGGAC 
AAGGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCTGGGC AGAGCAATGG GCGAGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TACGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762247.1
CDS	238..2820
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLERDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_008764025.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764025.2

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGGTGAGCA GGAACCGGGC CATTGCTACA CCCAACCAAG GTGTCTGGGA CATGCGTGGG 
AAACAGTTCT ACAATGGGAT TGAGATCAAA GTCTGGGCCA TCGCCTGCTT CGCACCCCAA 
AAACAATGTC GAGAAGAGGT GCTCAAGAAC TTCACGGACC AGCTTCGGAA GATCTCCAAG 
GATGCAGGGA TGCCCATCCA GGGTCAGCCA TGTTTCTGCA AATACGCACA GGGGGCGGAC 
AGCGTGGAGC CCATGTTCCG GCATCTCAAG AATACCTACT CAGGACTGCA GCTCATTATT 
GTCATCCTGC CCGGGAAGAC GCCGGTGTAT GCTGAAGTGA AGCGTGTTGG AGATACGCTC 
TTGGGAATGG CGACACAGTG TGTGCAGGTG AAGAATGTGG TCAAGACCTC ACCTCAGACT 
CTGTCCAACC TCTGCCTCAA GATCAATGTC AAACTCGGTG GCATCAACAA CATCCTTGTC 
CCACACCAGC GCTCTGCTGT TTTCCAACAG CCAGTGATTT TTCTGGGAGC AGACGTTACA 
CACCCCCCGG CTGGGGACGG GAAGAAACCA TCTATCACAG CAGTGGTAGG CAGTATGGAT 
GCACACCCCA GCCGATACTG TGCCACTGTG CGTGTACAGC GTCCACGGCA GGAGATCATT 
GAGGACTTGT CCTATATGGT GCGGGAGCTG CTCATCCAGT TCTACAAGTC CACCCGATTC 
AAGCCCACCC GAATCATCTT CTACCGAGAT GGGGTCCCTG AAGGCCAGCT GCCTCAGATC 
CTTCACTATG AGCTGCTTGC CATTCGAGAT GCCTGCATCA AACTGGAAAG AGACTACCAG 
CCTGGGATCA CGTACATCGT GGTACAGAAG CGACATCACA CCCGCCTCTT TTGTGCTGAC 
AAGAATGAGC GGATTGGGAA GAGTGGTAAC ATCCCAGCTG GGACCACTGT GGACACCAAC 
ATCACTCACC CGTTTGAGTT TGACTTCTAC CTGTGCAGCC ATGCAGGCAT CCAGGGCACC 
AGCCGACCAT CCCATTATTA TGTCCTTTGG GATGACAACC GTTTCACAGC AGATGAGCTC 
CAGATCTTGA CATACCAGCT GTGCCACACT TATGTACGAT GCACACGTTC TGTCTCTATC 
CCAGCACCTG CCTACTATGC CCGCCTGGTG GCTTTCCGGG CCCGATACCA CCTGGTGGAC 
AAGGAGCATG ACAGTGGAGA GGGGAGCCAC ATATCTGGGC AGAGCAATGG GCGAGACCCC 
CAGGCCCTGG CCAAAGCCGT GCAGGTTCAC CAGGATACTC TACGCACCAT GTACTTCGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( NM_001317181.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001304110.1 Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.

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CloneID	ORa63211
Clone ID Related Accession (Same CDS sequence)	NM_001317181.1
Accession Version	NM_001317181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-01
Organism	Rattus norvegicus(Norway rat)
Product	protein argonaute-1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125765.5 and FM038730.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCTAC ACCCAACCAA GGTGTCTGGG ACATGCGTGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAATGT 
CGAGAAGAGG TGCTCAAGAA CTTCACGGAC CAGCTTCGGA AGATCTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGTTTCTGC AAATACGCAC AGGGGGCGGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAATACCTAC TCAGGACTGC AGCTCATTAT TGTCATCCTG 
CCCGGGAAGA CGCCGGTGTA TGCTGAAGTG AAGCGTGTTG GAGATACGCT CTTGGGAATG 
GCGACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATCAACA ACATCCTTGT CCCACACCAG 
CGCTCTGCTG TTTTCCAACA GCCAGTGATT TTTCTGGGAG CAGACGTTAC ACACCCCCCG 
GCTGGGGACG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGTATGGA TGCACACCCC 
AGCCGATACT GTGCCACTGT GCGTGTACAG CGTCCACGGC AGGAGATCAT TGAGGACTTG 
TCCTATATGG TGCGGGAGCT GCTCATCCAG TTCTACAAGT CCACCCGATT CAAGCCCACC 
CGAATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TGCCTCAGAT CCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCCTGCATC AAACTGGAAA GAGACTACCA GCCTGGGATC 
ACGTACATCG TGGTACAGAA GCGACATCAC ACCCGCCTCT TTTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACTCAC 
CCGTTTGAGT TTGACTTCTA CCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAGCT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTATGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCCCGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCTGGG CAGAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA CAGAAGAAAG CTTTCCAAGC CCTGGGAGCT GTGCCACCCA 
AATCCAGAGG AAGCAAGGCA GAGGGAGCTG GGGTCAGATG GAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304110.1
CDS	204..2891
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLERDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*RQNAVTSLDRRKLSKPWELCHPNPEEARQRELGSDGT

Target ORF information:

RefSeq Version	NM_001317181.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argonaute RISC component 1 (Ago1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317181.1

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCA GGTGCCTACC TGCCTCCCCT GCAGCAGGTG 
TTCCAGGCCC CTCGCCGGCC TGGCATCGGC ACTGTGGGCA AACCGATCAA GCTTCTGGCC 
AATTACTTTG AGGTGGACAT TCCTAAGATT GACGTCTACC ATTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCTCGCAG AGTCAACCGG GAGGTGGTGG AGTACATGGT CCAGCATTTC 
AAACCGCAGA TCTTTGGGGA TCGCAAGCCT GTGTACGATG GAAAGAAGAA CATTTACACA 
GTTACAGCAC TGCCTATTGG CAATGAGAGG GTTGACTTTG AGGTGACCAT CCCTGGGGAA 
GGGAAGGATA GAATTTTTAA GGTCTCCATC AAGTGGCTAG CCATCGTGAG CTGGCGCATG 
CTGCATGAAG CCTTGGTCAG TGGCCAGATC CCTGTGCCCT TGGAGTCCGT GCAAGCCCTG 
GATGTGGCCA TGAGGCATCT GGCATCTATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTACA AAGCACAGCC AGTGATTGAG TTCATGTGTG AGGTCCTGGA CATCAGGAAC 
ATAGATGAAC AGCCCAAGCC CCTCACGGAT TCCCAGCGCG TTCGGTTTAC CAAGGAGATA 
AAGGGCCTGA AGGTGGAAGT GACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACGTTTCCCT TGCAGCTGGA GAGCGGACAG 
ACCGTGGAGT GCACCGTGGC ACAATATTTC AAGCAGAAAT ACAACCTTCA GCTCAAGTAT 
CCTCACCTGC CCTGCCTGCA AGTTGGGCAA GAGCAGAAGC ATACCTATTT GCCCCTCGAG 
GTCTGTAACA TTGTGGCTGG GCAGCGGTGC ATTAAGAAGC TGACTGACAA CCAGACTTCA 
ACCATGATAA AGGCTACAGC TAGGTCGGCC CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAACG CCAGCTACAA CTTGGATCCC TACATCCAGG AATTTGGAAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC GGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCTAC ACCCAACCAA GGTGTCTGGG ACATGCGTGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAATGT 
CGAGAAGAGG TGCTCAAGAA CTTCACGGAC CAGCTTCGGA AGATCTCCAA GGATGCAGGG 
ATGCCCATCC AGGGTCAGCC ATGTTTCTGC AAATACGCAC AGGGGGCGGA CAGCGTGGAG 
CCCATGTTCC GGCATCTCAA GAATACCTAC TCAGGACTGC AGCTCATTAT TGTCATCCTG 
CCCGGGAAGA CGCCGGTGTA TGCTGAAGTG AAGCGTGTTG GAGATACGCT CTTGGGAATG 
GCGACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTCGGT GGCATCAACA ACATCCTTGT CCCACACCAG 
CGCTCTGCTG TTTTCCAACA GCCAGTGATT TTTCTGGGAG CAGACGTTAC ACACCCCCCG 
GCTGGGGACG GGAAGAAACC ATCTATCACA GCAGTGGTAG GCAGTATGGA TGCACACCCC 
AGCCGATACT GTGCCACTGT GCGTGTACAG CGTCCACGGC AGGAGATCAT TGAGGACTTG 
TCCTATATGG TGCGGGAGCT GCTCATCCAG TTCTACAAGT CCACCCGATT CAAGCCCACC 
CGAATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TGCCTCAGAT CCTTCACTAT 
GAGCTGCTTG CCATTCGAGA TGCCTGCATC AAACTGGAAA GAGACTACCA GCCTGGGATC 
ACGTACATCG TGGTACAGAA GCGACATCAC ACCCGCCTCT TTTGTGCTGA CAAGAATGAG 
CGGATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACTG TGGACACCAA CATCACTCAC 
CCGTTTGAGT TTGACTTCTA CCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTATT ATGTCCTTTG GGATGACAAC CGTTTCACAG CAGATGAGCT CCAGATCTTG 
ACATACCAGC TGTGCCACAC TTATGTACGA TGCACACGTT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCCCGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCTGGG CAGAGCAATG GGCGAGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA CCAGGATACT CTACGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA CAGAAGAAAG CTTTCCAAGC CCTGGGAGCT GTGCCACCCA 
AATCCAGAGG AAGCAAGGCA GAGGGAGCTG GGGTCAGATG GAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ago1 ( XM_017593401.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448890.1 Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X2, mRNA.

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CloneID	ORa61237
Clone ID Related Accession (Same CDS sequence)	XM_017593401.1
Accession Version	XM_017593401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein argonaute-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GATGAACAGC CCAAGCCCCT CACGGATTCC 
CAGCGCGTTC GGTTTACCAA GGAGATAAAG GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGTC GCCCTGCTAG CCATCAGACG 
TTTCCCTTGC AGCTGGAGAG CGGACAGACC GTGGAGTGCA CCGTGGCACA ATATTTCAAG 
CAGAAATACA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTGCAAGT TGGGCAAGAG 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG TCGCCTGATG AAGAACGCCA GCTACAACTT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACGGG GCGAGTGCTG 
CCGGCGCCCA TCTTGCAGTA CGGCGGCCGG AACCGGGCCA TTGCTACACC CAACCAAGGT 
GTCTGGGACA TGCGTGGGAA ACAGTTCTAC AATGGGATTG AGATCAAAGT CTGGGCCATC 
GCCTGCTTCG CACCCCAAAA ACAATGTCGA GAAGAGGTGC TCAAGAACTT CACGGACCAG 
CTTCGGAAGA TCTCCAAGGA TGCAGGGATG CCCATCCAGG GTCAGCCATG TTTCTGCAAA 
TACGCACAGG GGGCGGACAG CGTGGAGCCC ATGTTCCGGC ATCTCAAGAA TACCTACTCA 
GGACTGCAGC TCATTATTGT CATCCTGCCC GGGAAGACGC CGGTGTATGC TGAAGTGAAG 
CGTGTTGGAG ATACGCTCTT GGGAATGGCG ACACAGTGTG TGCAGGTGAA GAATGTGGTC 
AAGACCTCAC CTCAGACTCT GTCCAACCTC TGCCTCAAGA TCAATGTCAA ACTCGGTGGC 
ATCAACAACA TCCTTGTCCC ACACCAGCGC TCTGCTGTTT TCCAACAGCC AGTGATTTTT 
CTGGGAGCAG ACGTTACACA CCCCCCGGCT GGGGACGGGA AGAAACCATC TATCACAGCA 
GTGGTAGGCA GTATGGATGC ACACCCCAGC CGATACTGTG CCACTGTGCG TGTACAGCGT 
CCACGGCAGG AGATCATTGA GGACTTGTCC TATATGGTGC GGGAGCTGCT CATCCAGTTC 
TACAAGTCCA CCCGATTCAA GCCCACCCGA ATCATCTTCT ACCGAGATGG GGTCCCTGAA 
GGCCAGCTGC CTCAGATCCT TCACTATGAG CTGCTTGCCA TTCGAGATGC CTGCATCAAA 
CTGGAAAGAG ACTACCAGCC TGGGATCACG TACATCGTGG TACAGAAGCG ACATCACACC 
CGCCTCTTTT GTGCTGACAA GAATGAGCGG ATTGGGAAGA GTGGTAACAT CCCAGCTGGG 
ACCACTGTGG ACACCAACAT CACTCACCCG TTTGAGTTTG ACTTCTACCT GTGCAGCCAT 
GCAGGCATCC AGGGCACCAG CCGACCATCC CATTATTATG TCCTTTGGGA TGACAACCGT 
TTCACAGCAG ATGAGCTCCA GATCTTGACA TACCAGCTGT GCCACACTTA TGTACGATGC 
ACACGTTCTG TCTCTATCCC AGCACCTGCC TACTATGCCC GCCTGGTGGC TTTCCGGGCC 
CGATACCACC TGGTGGACAA GGAGCATGAC AGTGGAGAGG GGAGCCACAT ATCTGGGCAG 
AGCAATGGGC GAGACCCCCA GGCCCTGGCC AAAGCCGTGC AGGTTCACCA GGATACTCTA 
CGCACCATGT ACTTCGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448890.1
CDS	111..2231
Translation	MPHRVGACARGTSSLPVPARYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLERDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	XM_017593401.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argonaute 1, RISC catalytic component (Ago1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593401.1

ORF Insert Sequence:

ATGCCTCACA GGGTGGGGGC CTGTGCCCGA GGGACCAGTT CTCTGCCTGT CCCTGCCAGG 
TACACCCCTG TGGGCCGCTC CTTCTTCTCA CCGCCTGAGG GCTACTACCA CCCGCTGGGG 
GGTGGGCGCG AGGTCTGGTT CGGCTTTCAC CAGTCTGTGC GCCCTGCCAT GTGGAAGATG 
ATGCTCAACA TTGATGTCTC AGCCACTGCC TTCTACAAAG CACAGCCAGT GATTGAGTTC 
ATGTGTGAGG TCCTGGACAT CAGGAACATA GATGAACAGC CCAAGCCCCT CACGGATTCC 
CAGCGCGTTC GGTTTACCAA GGAGATAAAG GGCCTGAAGG TGGAAGTGAC CCACTGTGGA 
CAGATGAAGA GGAAATACCG CGTGTGTAAT GTTACCCGTC GCCCTGCTAG CCATCAGACG 
TTTCCCTTGC AGCTGGAGAG CGGACAGACC GTGGAGTGCA CCGTGGCACA ATATTTCAAG 
CAGAAATACA ACCTTCAGCT CAAGTATCCT CACCTGCCCT GCCTGCAAGT TGGGCAAGAG 
CAGAAGCATA CCTATTTGCC CCTCGAGGTC TGTAACATTG TGGCTGGGCA GCGGTGCATT 
AAGAAGCTGA CTGACAACCA GACTTCAACC ATGATAAAGG CTACAGCTAG GTCGGCCCCA 
GACAGACAGG AGGAGATCAG TCGCCTGATG AAGAACGCCA GCTACAACTT GGATCCCTAC 
ATCCAGGAAT TTGGAATCAA AGTGAAGGAT GACATGACGG AGGTGACGGG GCGAGTGCTG 
CCGGCGCCCA TCTTGCAGTA CGGCGGCCGG AACCGGGCCA TTGCTACACC CAACCAAGGT 
GTCTGGGACA TGCGTGGGAA ACAGTTCTAC AATGGGATTG AGATCAAAGT CTGGGCCATC 
GCCTGCTTCG CACCCCAAAA ACAATGTCGA GAAGAGGTGC TCAAGAACTT CACGGACCAG 
CTTCGGAAGA TCTCCAAGGA TGCAGGGATG CCCATCCAGG GTCAGCCATG TTTCTGCAAA 
TACGCACAGG GGGCGGACAG CGTGGAGCCC ATGTTCCGGC ATCTCAAGAA TACCTACTCA 
GGACTGCAGC TCATTATTGT CATCCTGCCC GGGAAGACGC CGGTGTATGC TGAAGTGAAG 
CGTGTTGGAG ATACGCTCTT GGGAATGGCG ACACAGTGTG TGCAGGTGAA GAATGTGGTC 
AAGACCTCAC CTCAGACTCT GTCCAACCTC TGCCTCAAGA TCAATGTCAA ACTCGGTGGC 
ATCAACAACA TCCTTGTCCC ACACCAGCGC TCTGCTGTTT TCCAACAGCC AGTGATTTTT 
CTGGGAGCAG ACGTTACACA CCCCCCGGCT GGGGACGGGA AGAAACCATC TATCACAGCA 
GTGGTAGGCA GTATGGATGC ACACCCCAGC CGATACTGTG CCACTGTGCG TGTACAGCGT 
CCACGGCAGG AGATCATTGA GGACTTGTCC TATATGGTGC GGGAGCTGCT CATCCAGTTC 
TACAAGTCCA CCCGATTCAA GCCCACCCGA ATCATCTTCT ACCGAGATGG GGTCCCTGAA 
GGCCAGCTGC CTCAGATCCT TCACTATGAG CTGCTTGCCA TTCGAGATGC CTGCATCAAA 
CTGGAAAGAG ACTACCAGCC TGGGATCACG TACATCGTGG TACAGAAGCG ACATCACACC 
CGCCTCTTTT GTGCTGACAA GAATGAGCGG ATTGGGAAGA GTGGTAACAT CCCAGCTGGG 
ACCACTGTGG ACACCAACAT CACTCACCCG TTTGAGTTTG ACTTCTACCT GTGCAGCCAT 
GCAGGCATCC AGGGCACCAG CCGACCATCC CATTATTATG TCCTTTGGGA TGACAACCGT 
TTCACAGCAG ATGAGCTCCA GATCTTGACA TACCAGCTGT GCCACACTTA TGTACGATGC 
ACACGTTCTG TCTCTATCCC AGCACCTGCC TACTATGCCC GCCTGGTGGC TTTCCGGGCC 
CGATACCACC TGGTGGACAA GGAGCATGAC AGTGGAGAGG GGAGCCACAT ATCTGGGCAG 
AGCAATGGGC GAGACCCCCA GGCCCTGGCC AAAGCCGTGC AGGTTCACCA GGATACTCTA 
CGCACCATGT ACTTCGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology25(2)120-6(2016 Mar-Apr)
Sun S,Ma J,Zhang Q,Wang Q,Zhou L,Bai F,Hu H,Chang P,Yu J,Gao B

Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA.
The Journal of biological chemistry291(13)7119-27(2016 Mar)
Ghosh U,Adhya S

Cellular microRNAs up-regulate transcription via interaction with promoter TATA-box motifs.
RNA (New York, N.Y.)20(12)1878-89(2014 Dec)
Zhang Y,Fan M,Zhang X,Huang F,Wu K,Zhang J,Liu J,Huang Z,Luo H,Tao L,Zhang H

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

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Ago1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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