SGPL1 cDNA ORF clone, Bos taurus(cattle)

The following SGPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa34215 NM_001098053.1
Latest version!
Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa34215 XM_005226395.4
Latest version!
Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa34215 XM_005226397.4
Latest version!
Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa34215 XM_005226396.3
Latest version!
Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
OBa34215 XM_005226397.3 Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa34215 XM_005226395.3 Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version NM_001098053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140468.1. On May 23, 2007 this sequence version replaced XM_600800.3. ##Evidence-Data-START## Transcript exon combination :: BC140468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091522.1
    CDS172..1878
    Translation

    Target ORF information:

    RefSeq Version NM_001098053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version XM_005226396.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226453.1
    CDS857..2563
    Translation

    Target ORF information:

    RefSeq Version XM_005226396.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226396.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version XM_005226397.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226454.1
    CDS77..1783
    Translation

    Target ORF information:

    RefSeq Version XM_005226397.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226397.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version XM_005226395.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226395.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226452.1
    CDS188..1894
    Translation

    Target ORF information:

    RefSeq Version XM_005226395.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226395.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version XM_005226397.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226397.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226454.1
    CDS92..1798
    Translation

    Target ORF information:

    RefSeq Version XM_005226397.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226397.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34215
    Clone ID Related Accession (Same CDS sequence) XM_005226396.3 , NM_001098053.1 , XM_005226397.3 , XM_005226395.4 , XM_005226397.4 , XM_005226395.3
    Accession Version XM_005226395.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226452.1
    CDS156..1862
    Translation

    Target ORF information:

    RefSeq Version XM_005226395.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine-1-phosphate lyase 1 (SGPL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226395.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCCCAGTG CAGACCTAAT GTCGTTGAAG GCCTTTGAGC CCTACTTTGA GATTTTGGAA 
    GTATATTCCA CAAAGGCCAA GAACTACGTG AATGGGCACT GTACCAAGTA TGAGCCCTGG
    CAGCTGATTG TGTGGAGTGT CGTGTGGACT CTGCTGATAG TCTGGGTGTA TGAGTTTGTC
    TTCCAGCCAG AGAGTTTATG GTCAAGGTTT AAGAAGAGAT GTTTTAAGCT TATCCGGAAG
    ATGCCTATCA TTGGTCGTAA GATCCAAGAT AAGTTGAACA AGACCAAGGA AGATATTAGC
    AAGAGCATGA CATTCCTGAA AGTGGACCAA GATTACGTGA AAGCCCTGCC CTCTCAAGGC
    CTGAGCCCGG CTGCCGTTCT GGAGAAACTC AAAGAATACA GCTCGAAGGA TGTCTTGTGG
    CAAGAGGGGA AAGCCTCCGG AGCAGTGTAT AGTGGGGAGA AGGAGCTCAC CGACCTCCTC
    GTGAAGGCTT ATGGAGATTT CGCATGGAGT AATCCACTGC ATCCAGACAT CTTCCCCGGA
    CTCCGGAAGA TAGAGGCAGA GATTGTGAGG ATGGCTTGTT CCCTATTCAA TGGGGGACCA
    GATTCCTGTG GATGTGTGAC CTCTGGGGGG ACAGAAAGTA TTTTGATGGC CTGCAAAGCT
    TATCGGGAAC TAGCCTTCGA GAATGGGATC AAAACTCCAG AAATCGTAGC CCCCCAGAGT
    GCCCACGCTG CATTTGACAA AGCCGCCAGT TACTTTGGGA TGAAGATTAT ACGGGTTCCC
    CTAAACAAAA TGATGGAGGT GGATGTTCGG GCAATGCGCA GAGCCATCTC TAGGAATACG
    GCCATGCTCG TCTGTTCTGC CCCACAGTTC CCTCATGGTG TGATAGACCC TATCCCTGAA
    GTGGCCAAGC TGGCTGTCAA ATACAAAATA CCTCTTCATG TGGACGCTTG TCTGGGGGGC
    TTCCTCATCG TCTTCATGGA GAAAGCGGGA TACCCGCTGG AGCAGCCGTT TGATTTCCGG
    GTGAAAGGCG TGACCAGCAT TTCAGCTGAT ACTCATAAGT ATGGCTACGC CCCCAAGGGC
    TCTTCCGTGC TCTTGTACTC TGACAAGAAG TACAGGAGCT ATCAGTTCTT CGTTGCCACA
    GACTGGCAGG GCGGCATCTA CGCTTCCCCA ACCATTGCGG GCTCACGGCC TGGCGGCATC
    AGCGCAGCCT GTTGGGCCTC CTTGATGTAC TTCGGTGAGA GCGGCTACGT TGAAGCTACC
    AAACAGATCA TCAAAACCAC TCGCTTCCTC AAGTCAGAAC TAGAAAATAT CAAAGGCATC
    TTTGTCTTTG GGAATCCTCA GTTGTCAGTC ATCGCTCTGG GCTCTCGTGA TTTTGATATC
    TATCGACTAT TCAACCTGAT GAATGCCAAG GGCTGGAACT TGAATCAGCT GCAGTTCCCA
    CCCAGCCTTC ATTTCTGCAT CACATTGGTA CACACCCGCA AGCGGGTAGC CATACAGTTC
    CTTAAGGATA TCCGGGAATC CGTCACTCAA ATCATGAAGA ATCCGAAAGC AAAGACCACA
    GGAATGGGTG CGATCTATGG CATGGCCCAG GCTACCATCG ACAGGAACCT GGTGGCGGAG
    TTGTCCTCCG TCTTCTTGGA CAGCCTTTTC AGTACAGACA CTACAACTCA GGGCAGCCAG
    ATGAATGGCT CTCCGAAACC CCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.