sgpl1 cDNA ORF clone, Danio rerio(zebrafish)

The following sgpl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sgpl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa35227 NM_001089469.1
Latest version!
Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa18680 XM_005156720.4
Latest version!
Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa18680 XM_005156720.3 Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa35227
    Clone ID Related Accession (Same CDS sequence) NM_001089469.1
    Accession Version NM_001089469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Danio rerio(zebrafish)
    Product sphingosine-1-phosphate lyase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139528.1. ##Evidence-Data-START## Transcript exon combination :: BC139528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTACT GGAGTGCCTT AGAGGTGTAC AAGGAGACTG CGCTGTTGTA CATCGAGGAG 
    GCGCAGAGTT ATGTGAATTC TCAGTGTGCT GGTCTGGAGC CATGGCAGAT CATTGCAGCC
    ACTTTGCTGT CCACACTCGG GGTAGTATGG CTAAAGGACT TTCTTTTCCA ACAAGAGAGT
    CTGACGTCAA GAGTAAAGAA GCAATTCTTC AGAATCATCA GAAAAATCCC ATTTATTGGA
    GCATCAATTC AGAATCAGCT CAACAAAGCT CTGGATGACA TGTCAATGAG TCTGTGTACG
    CTGAAGGAGG GCATGAGCTA CACCAAACTC CTGCCAGCGC AGGGCCTCAC CCACAAACAG
    CTCCTTGACA AGATCAGGGA GTATGAGACA CTGAGTGAGG TGAACTGGGC GAAAGGCAAA
    GTCTCGGGTG CTGTTTACTG GGGGGATGAG AAGCTCACAG ATCTCCTGGT GAAGGTGTAT
    GGGGAGTTTG CCTGGACCAA CCCTCTCCAC CCAGACATAT TCCCTGGAGT TAGGAAGATG
    GAGGCAGAAG TGGTGCGGAT GACATGTGCC CTCTTTAATG GAGGACCAGA CTCCTGCGGC
    ACAGTTACTT CAGGGGGAAC CGAAAGCATC CTGATGGCAT GCAAGGCATA CAGAGACATG
    GCTCATGAAC GTGGCATCAA ACACCCGGAG ATCATTGCAC CAATCAGTGT CCATGCGGCG
    TTTGACAAAG CAGCACATTA CTTTGGGATG AAACTCATTC ATGTACCGTT GGACAATAAG
    ACCATGAAAG TTGATGTGAA GGCCATGAGG AGAGCCATCA CTAAAAACAC AGCAATGCTG
    GTTTGTTCAG CTCCTCAGTT CCCCCATGGA ATCATGGATC CTGTAGAAGA AGTGGCAAAG
    CTTGCAGTAA AGTATAACAT CCCATTCCAC GTGGATGCAT GCTTGGGTGG GTTCCTGATA
    GTGTTTATGG AAAAAGCAGG TTTCAAGCTG GCTCCTTTTG ATTTCCGGGT CAAAGGCGTG
    ACCAGTATCT CAGCTGATAC ACATAAGTAT GGTTATGCTC CCAAAGGCTC CTCAGTTGTG
    CTCTACAGCA ACAGGAAGTT TCGCCACTAC CAGTATTTTG TAGCACCTGA TTGGCAGGGA
    GGAATTTATG CCTCTCCATC CATGGCTGGC TCTCGGCCTG GAGGCATCAT TGCTGCCTGC
    TGGGCTACTA TGATGCACAT GGGTGAGAAA GGATACGTTG AGGCCACCAG GAAAGTTGTG
    GAAACAACTC GCAAAATCAA AACGGGAATC CGTAAAATAG ATGGGGTGTT TGTATTTGGG
    GATCCAGAGG TGTCTGTGGT GGCTCTGGGC TCAGATGATT TTGATATCTT TCGCTTATCA
    AATGCACTGA CTTCAAAGGG CTGGAATCTC AACACTCTGC AGTTTCCATC TAGCATCCAT
    ATTTGTGTCA CAATGCTGCA CACACAGTCT GGTGTAGCGG AGCAGTTCAT TAGTGACGTG
    AAGAAAGAAG TAGCCATTAT TATGAAGAAT CCGAAAGAGA AGACCACAGG AATGGGAGCC
    ATCTACGGCA TGGCCCAGTC CATTCCTGAC CGATCGATGG TGACCGAGGT CTCACAGGGC
    TTTTTGGACT GCCTCTACAG CACTGAGGTG CCCAAGATGC AAAACAAGCA CCACAAAAAC
    CACAAGAATC ACATAAACGG CAACTCCAAA GTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001082938.1
    CDS162..1880
    Translation

    Target ORF information:

    RefSeq Version NM_001089469.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001089469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTACT GGAGTGCCTT AGAGGTGTAC AAGGAGACTG CGCTGTTGTA CATCGAGGAG 
    GCGCAGAGTT ATGTGAATTC TCAGTGTGCT GGTCTGGAGC CATGGCAGAT CATTGCAGCC
    ACTTTGCTGT CCACACTCGG GGTAGTATGG CTAAAGGACT TTCTTTTCCA ACAAGAGAGT
    CTGACGTCAA GAGTAAAGAA GCAATTCTTC AGAATCATCA GAAAAATCCC ATTTATTGGA
    GCATCAATTC AGAATCAGCT CAACAAAGCT CTGGATGACA TGTCAATGAG TCTGTGTACG
    CTGAAGGAGG GCATGAGCTA CACCAAACTC CTGCCAGCGC AGGGCCTCAC CCACAAACAG
    CTCCTTGACA AGATCAGGGA GTATGAGACA CTGAGTGAGG TGAACTGGGC GAAAGGCAAA
    GTCTCGGGTG CTGTTTACTG GGGGGATGAG AAGCTCACAG ATCTCCTGGT GAAGGTGTAT
    GGGGAGTTTG CCTGGACCAA CCCTCTCCAC CCAGACATAT TCCCTGGAGT TAGGAAGATG
    GAGGCAGAAG TGGTGCGGAT GACATGTGCC CTCTTTAATG GAGGACCAGA CTCCTGCGGC
    ACAGTTACTT CAGGGGGAAC CGAAAGCATC CTGATGGCAT GCAAGGCATA CAGAGACATG
    GCTCATGAAC GTGGCATCAA ACACCCGGAG ATCATTGCAC CAATCAGTGT CCATGCGGCG
    TTTGACAAAG CAGCACATTA CTTTGGGATG AAACTCATTC ATGTACCGTT GGACAATAAG
    ACCATGAAAG TTGATGTGAA GGCCATGAGG AGAGCCATCA CTAAAAACAC AGCAATGCTG
    GTTTGTTCAG CTCCTCAGTT CCCCCATGGA ATCATGGATC CTGTAGAAGA AGTGGCAAAG
    CTTGCAGTAA AGTATAACAT CCCATTCCAC GTGGATGCAT GCTTGGGTGG GTTCCTGATA
    GTGTTTATGG AAAAAGCAGG TTTCAAGCTG GCTCCTTTTG ATTTCCGGGT CAAAGGCGTG
    ACCAGTATCT CAGCTGATAC ACATAAGTAT GGTTATGCTC CCAAAGGCTC CTCAGTTGTG
    CTCTACAGCA ACAGGAAGTT TCGCCACTAC CAGTATTTTG TAGCACCTGA TTGGCAGGGA
    GGAATTTATG CCTCTCCATC CATGGCTGGC TCTCGGCCTG GAGGCATCAT TGCTGCCTGC
    TGGGCTACTA TGATGCACAT GGGTGAGAAA GGATACGTTG AGGCCACCAG GAAAGTTGTG
    GAAACAACTC GCAAAATCAA AACGGGAATC CGTAAAATAG ATGGGGTGTT TGTATTTGGG
    GATCCAGAGG TGTCTGTGGT GGCTCTGGGC TCAGATGATT TTGATATCTT TCGCTTATCA
    AATGCACTGA CTTCAAAGGG CTGGAATCTC AACACTCTGC AGTTTCCATC TAGCATCCAT
    ATTTGTGTCA CAATGCTGCA CACACAGTCT GGTGTAGCGG AGCAGTTCAT TAGTGACGTG
    AAGAAAGAAG TAGCCATTAT TATGAAGAAT CCGAAAGAGA AGACCACAGG AATGGGAGCC
    ATCTACGGCA TGGCCCAGTC CATTCCTGAC CGATCGATGG TGACCGAGGT CTCACAGGGC
    TTTTTGGACT GCCTCTACAG CACTGAGGTG CCCAAGATGC AAAACAAGCA CCACAAAAAC
    CACAAGAATC ACATAAACGG CAACTCCAAA GTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18680
    Clone ID Related Accession (Same CDS sequence) XM_005156720.3 , XM_005156720.4
    Accession Version XM_005156720.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGACTCAGT CTGCTATAAG TGCCTTAGAG GTGTACAAGG AGACTGCGCT GTTGTACATC 
    GAGGAGGCGC AGAGTTATGT GAATTCTCAG TGTGCTGGTC TGGAGCCATG GCAGATCATT
    GCAGCCACTT TGCTGTCCAC ACTCGGGGTA GTATGGCTAA AGGACTTTCT TTTCCAACAA
    GAGAGTCTGA CGTCAAGAGT AAAGAAGCAA TTCTTCAGAA TCATCAGAAA AATCCCATTT
    ATTGGAGCAT CAATTCAGAA TCAGCTCAAC AAAGCTCTGG ATGACATGTC AATGAGTCTG
    TGTACGCTGA AGGAGGGCAT GAGCTACACC AAACTCCTGC CAGCGCAGGG CCTCACCCAC
    AAACAGCTCC TTGACAAGAT CAGGGAGTAT GAGACACTGA GTGAGGTGAA CTGGGCGAAA
    GGCAAAGTCT CGGGTGCTGT TTACTGGGGG GATGAGAAGC TCACAGATCT CCTGGTGAAG
    GTGTATGGGG AGTTTGCCTG GACCAACCCT CTCCACCCAG ACATATTCCC TGGAGTTAGG
    AAGATGGAGG CAGAAGTGGT GCGGATGACA TGTGCCCTCT TTAATGGAGG ACCAGACTCC
    TGCGGCACAG TTACTTCAGG GGGAACCGAA AGCATCCTGA TGGCATGCAA GGCATACAGA
    GACATGGCTC ATGAACGTGG CATCAAACAC CCGGAGATCA TTGCACCAAT CAGTGTCCAT
    GCGGCGTTTG ACAAAGCAGC ACATTACTTT GGGATGAAAC TCATTCATGT ACCGTTGGAC
    AATAAGACCA TGAAAGTTGA TGTGAAGGCC ATGAGGAGAG CCATCACTAA AAACACAGCA
    ATGCTGGTTT GTTCAGCTCC TCAGTTCCCC CATGGAATCA TGGATCCTGT AGAAGAAGTG
    GCAAAGCTTG CAGTAAAGTA TAACATCCCA TTCCACGTGG ATGCATGCTT GGGTGGGTTC
    CTGATAGTGT TTATGGAAAA AGCAGGTTTC AAGCTGGCTC CTTTTGATTT CCGGGTCAAA
    GGCGTGACCA GTATCTCAGC TGATACACAT AAGTATGGTT ATGCTCCCAA AGGCTCCTCA
    GTTGTGCTCT ACAGCAACAG GAAGTTTCGC CACTACCAGT ATTTTGTAGC ACCTGATTGG
    CAGGGAGGAA TTTATGCCTC TCCATCCATG GCTGGCTCTC GGCCTGGAGG CATCATTGCT
    GCCTGCTGGG CTACTATGAT GCACATGGGT GAGAAAGGAT ACGTTGAGGC CACCAGGAAA
    GTTGTGGAAA CAACTCGCAA AATCAAAACG GGAATCCGTA AAATAGATGG GGTGTTTGTA
    TTTGGGGATC CAGAGGTGTC TGTGGTGGCT CTGGGCTCAG ATGATTTTGA TATCTTTCGC
    TTATCAAATG CACTGACTTC AAAGGGCTGG AATCTCAACA CTCTGCAGTT TCCATCTAGC
    ATCCATATTT GTGTCACAAT GCTGCACACA CAGTCTGGTG TAGCGGAGCA GTTCATTAGT
    GACGTGAAGA AAGAAGTAGC CATTATTATG AAGAATCCGA AAGAGAAGAC CACAGGAATG
    GGAGCCATCT ACGGCATGGC CCAGTCCATT CCTGACCGAT CGATGGTGAC CGAGGTCTCA
    CAGGGCTTTT TGGACTGCCT CTACAGCACT GAGGTGCCCA AGATGCAAAA CAAGCACCAC
    AAAAACCACA AGAATCACAT AAACGGCAAC TCCAAAGTGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156777.1
    CDS61..1785
    Translation

    Target ORF information:

    RefSeq Version XM_005156720.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGACTCAGT CTGCTATAAG TGCCTTAGAG GTGTACAAGG AGACTGCGCT GTTGTACATC 
    GAGGAGGCGC AGAGTTATGT GAATTCTCAG TGTGCTGGTC TGGAGCCATG GCAGATCATT
    GCAGCCACTT TGCTGTCCAC ACTCGGGGTA GTATGGCTAA AGGACTTTCT TTTCCAACAA
    GAGAGTCTGA CGTCAAGAGT AAAGAAGCAA TTCTTCAGAA TCATCAGAAA AATCCCATTT
    ATTGGAGCAT CAATTCAGAA TCAGCTCAAC AAAGCTCTGG ATGACATGTC AATGAGTCTG
    TGTACGCTGA AGGAGGGCAT GAGCTACACC AAACTCCTGC CAGCGCAGGG CCTCACCCAC
    AAACAGCTCC TTGACAAGAT CAGGGAGTAT GAGACACTGA GTGAGGTGAA CTGGGCGAAA
    GGCAAAGTCT CGGGTGCTGT TTACTGGGGG GATGAGAAGC TCACAGATCT CCTGGTGAAG
    GTGTATGGGG AGTTTGCCTG GACCAACCCT CTCCACCCAG ACATATTCCC TGGAGTTAGG
    AAGATGGAGG CAGAAGTGGT GCGGATGACA TGTGCCCTCT TTAATGGAGG ACCAGACTCC
    TGCGGCACAG TTACTTCAGG GGGAACCGAA AGCATCCTGA TGGCATGCAA GGCATACAGA
    GACATGGCTC ATGAACGTGG CATCAAACAC CCGGAGATCA TTGCACCAAT CAGTGTCCAT
    GCGGCGTTTG ACAAAGCAGC ACATTACTTT GGGATGAAAC TCATTCATGT ACCGTTGGAC
    AATAAGACCA TGAAAGTTGA TGTGAAGGCC ATGAGGAGAG CCATCACTAA AAACACAGCA
    ATGCTGGTTT GTTCAGCTCC TCAGTTCCCC CATGGAATCA TGGATCCTGT AGAAGAAGTG
    GCAAAGCTTG CAGTAAAGTA TAACATCCCA TTCCACGTGG ATGCATGCTT GGGTGGGTTC
    CTGATAGTGT TTATGGAAAA AGCAGGTTTC AAGCTGGCTC CTTTTGATTT CCGGGTCAAA
    GGCGTGACCA GTATCTCAGC TGATACACAT AAGTATGGTT ATGCTCCCAA AGGCTCCTCA
    GTTGTGCTCT ACAGCAACAG GAAGTTTCGC CACTACCAGT ATTTTGTAGC ACCTGATTGG
    CAGGGAGGAA TTTATGCCTC TCCATCCATG GCTGGCTCTC GGCCTGGAGG CATCATTGCT
    GCCTGCTGGG CTACTATGAT GCACATGGGT GAGAAAGGAT ACGTTGAGGC CACCAGGAAA
    GTTGTGGAAA CAACTCGCAA AATCAAAACG GGAATCCGTA AAATAGATGG GGTGTTTGTA
    TTTGGGGATC CAGAGGTGTC TGTGGTGGCT CTGGGCTCAG ATGATTTTGA TATCTTTCGC
    TTATCAAATG CACTGACTTC AAAGGGCTGG AATCTCAACA CTCTGCAGTT TCCATCTAGC
    ATCCATATTT GTGTCACAAT GCTGCACACA CAGTCTGGTG TAGCGGAGCA GTTCATTAGT
    GACGTGAAGA AAGAAGTAGC CATTATTATG AAGAATCCGA AAGAGAAGAC CACAGGAATG
    GGAGCCATCT ACGGCATGGC CCAGTCCATT CCTGACCGAT CGATGGTGAC CGAGGTCTCA
    CAGGGCTTTT TGGACTGCCT CTACAGCACT GAGGTGCCCA AGATGCAAAA CAAGCACCAC
    AAAAACCACA AGAATCACAT AAACGGCAAC TCCAAAGTGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18680
    Clone ID Related Accession (Same CDS sequence) XM_005156720.3 , XM_005156720.4
    Accession Version XM_005156720.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sphingosine-1-phosphate lyase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGACTCAGT CTGCTATAAG TGCCTTAGAG GTGTACAAGG AGACTGCGCT GTTGTACATC 
    GAGGAGGCGC AGAGTTATGT GAATTCTCAG TGTGCTGGTC TGGAGCCATG GCAGATCATT
    GCAGCCACTT TGCTGTCCAC ACTCGGGGTA GTATGGCTAA AGGACTTTCT TTTCCAACAA
    GAGAGTCTGA CGTCAAGAGT AAAGAAGCAA TTCTTCAGAA TCATCAGAAA AATCCCATTT
    ATTGGAGCAT CAATTCAGAA TCAGCTCAAC AAAGCTCTGG ATGACATGTC AATGAGTCTG
    TGTACGCTGA AGGAGGGCAT GAGCTACACC AAACTCCTGC CAGCGCAGGG CCTCACCCAC
    AAACAGCTCC TTGACAAGAT CAGGGAGTAT GAGACACTGA GTGAGGTGAA CTGGGCGAAA
    GGCAAAGTCT CGGGTGCTGT TTACTGGGGG GATGAGAAGC TCACAGATCT CCTGGTGAAG
    GTGTATGGGG AGTTTGCCTG GACCAACCCT CTCCACCCAG ACATATTCCC TGGAGTTAGG
    AAGATGGAGG CAGAAGTGGT GCGGATGACA TGTGCCCTCT TTAATGGAGG ACCAGACTCC
    TGCGGCACAG TTACTTCAGG GGGAACCGAA AGCATCCTGA TGGCATGCAA GGCATACAGA
    GACATGGCTC ATGAACGTGG CATCAAACAC CCGGAGATCA TTGCACCAAT CAGTGTCCAT
    GCGGCGTTTG ACAAAGCAGC ACATTACTTT GGGATGAAAC TCATTCATGT ACCGTTGGAC
    AATAAGACCA TGAAAGTTGA TGTGAAGGCC ATGAGGAGAG CCATCACTAA AAACACAGCA
    ATGCTGGTTT GTTCAGCTCC TCAGTTCCCC CATGGAATCA TGGATCCTGT AGAAGAAGTG
    GCAAAGCTTG CAGTAAAGTA TAACATCCCA TTCCACGTGG ATGCATGCTT GGGTGGGTTC
    CTGATAGTGT TTATGGAAAA AGCAGGTTTC AAGCTGGCTC CTTTTGATTT CCGGGTCAAA
    GGCGTGACCA GTATCTCAGC TGATACACAT AAGTATGGTT ATGCTCCCAA AGGCTCCTCA
    GTTGTGCTCT ACAGCAACAG GAAGTTTCGC CACTACCAGT ATTTTGTAGC ACCTGATTGG
    CAGGGAGGAA TTTATGCCTC TCCATCCATG GCTGGCTCTC GGCCTGGAGG CATCATTGCT
    GCCTGCTGGG CTACTATGAT GCACATGGGT GAGAAAGGAT ACGTTGAGGC CACCAGGAAA
    GTTGTGGAAA CAACTCGCAA AATCAAAACG GGAATCCGTA AAATAGATGG GGTGTTTGTA
    TTTGGGGATC CAGAGGTGTC TGTGGTGGCT CTGGGCTCAG ATGATTTTGA TATCTTTCGC
    TTATCAAATG CACTGACTTC AAAGGGCTGG AATCTCAACA CTCTGCAGTT TCCATCTAGC
    ATCCATATTT GTGTCACAAT GCTGCACACA CAGTCTGGTG TAGCGGAGCA GTTCATTAGT
    GACGTGAAGA AAGAAGTAGC CATTATTATG AAGAATCCGA AAGAGAAGAC CACAGGAATG
    GGAGCCATCT ACGGCATGGC CCAGTCCATT CCTGACCGAT CGATGGTGAC CGAGGTCTCA
    CAGGGCTTTT TGGACTGCCT CTACAGCACT GAGGTGCCCA AGATGCAAAA CAAGCACCAC
    AAAAACCACA AGAATCACAT AAACGGCAAC TCCAAAGTGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156777.1
    CDS126..1850
    Translation

    Target ORF information:

    RefSeq Version XM_005156720.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sphingosine-1-phosphate lyase 1 (sgpl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156720.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGACTCAGT CTGCTATAAG TGCCTTAGAG GTGTACAAGG AGACTGCGCT GTTGTACATC 
    GAGGAGGCGC AGAGTTATGT GAATTCTCAG TGTGCTGGTC TGGAGCCATG GCAGATCATT
    GCAGCCACTT TGCTGTCCAC ACTCGGGGTA GTATGGCTAA AGGACTTTCT TTTCCAACAA
    GAGAGTCTGA CGTCAAGAGT AAAGAAGCAA TTCTTCAGAA TCATCAGAAA AATCCCATTT
    ATTGGAGCAT CAATTCAGAA TCAGCTCAAC AAAGCTCTGG ATGACATGTC AATGAGTCTG
    TGTACGCTGA AGGAGGGCAT GAGCTACACC AAACTCCTGC CAGCGCAGGG CCTCACCCAC
    AAACAGCTCC TTGACAAGAT CAGGGAGTAT GAGACACTGA GTGAGGTGAA CTGGGCGAAA
    GGCAAAGTCT CGGGTGCTGT TTACTGGGGG GATGAGAAGC TCACAGATCT CCTGGTGAAG
    GTGTATGGGG AGTTTGCCTG GACCAACCCT CTCCACCCAG ACATATTCCC TGGAGTTAGG
    AAGATGGAGG CAGAAGTGGT GCGGATGACA TGTGCCCTCT TTAATGGAGG ACCAGACTCC
    TGCGGCACAG TTACTTCAGG GGGAACCGAA AGCATCCTGA TGGCATGCAA GGCATACAGA
    GACATGGCTC ATGAACGTGG CATCAAACAC CCGGAGATCA TTGCACCAAT CAGTGTCCAT
    GCGGCGTTTG ACAAAGCAGC ACATTACTTT GGGATGAAAC TCATTCATGT ACCGTTGGAC
    AATAAGACCA TGAAAGTTGA TGTGAAGGCC ATGAGGAGAG CCATCACTAA AAACACAGCA
    ATGCTGGTTT GTTCAGCTCC TCAGTTCCCC CATGGAATCA TGGATCCTGT AGAAGAAGTG
    GCAAAGCTTG CAGTAAAGTA TAACATCCCA TTCCACGTGG ATGCATGCTT GGGTGGGTTC
    CTGATAGTGT TTATGGAAAA AGCAGGTTTC AAGCTGGCTC CTTTTGATTT CCGGGTCAAA
    GGCGTGACCA GTATCTCAGC TGATACACAT AAGTATGGTT ATGCTCCCAA AGGCTCCTCA
    GTTGTGCTCT ACAGCAACAG GAAGTTTCGC CACTACCAGT ATTTTGTAGC ACCTGATTGG
    CAGGGAGGAA TTTATGCCTC TCCATCCATG GCTGGCTCTC GGCCTGGAGG CATCATTGCT
    GCCTGCTGGG CTACTATGAT GCACATGGGT GAGAAAGGAT ACGTTGAGGC CACCAGGAAA
    GTTGTGGAAA CAACTCGCAA AATCAAAACG GGAATCCGTA AAATAGATGG GGTGTTTGTA
    TTTGGGGATC CAGAGGTGTC TGTGGTGGCT CTGGGCTCAG ATGATTTTGA TATCTTTCGC
    TTATCAAATG CACTGACTTC AAAGGGCTGG AATCTCAACA CTCTGCAGTT TCCATCTAGC
    ATCCATATTT GTGTCACAAT GCTGCACACA CAGTCTGGTG TAGCGGAGCA GTTCATTAGT
    GACGTGAAGA AAGAAGTAGC CATTATTATG AAGAATCCGA AAGAGAAGAC CACAGGAATG
    GGAGCCATCT ACGGCATGGC CCAGTCCATT CCTGACCGAT CGATGGTGAC CGAGGTCTCA
    CAGGGCTTTT TGGACTGCCT CTACAGCACT GAGGTGCCCA AGATGCAAAA CAAGCACCAC
    AAAAACCACA AGAATCACAT AAACGGCAAC TCCAAAGTGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.