Sply cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sply
Entrez Gene ID	46059
Full Name	Sphingosine-1-phosphate lyase
Synonyms	CG8946,Dmel\CG8946,SPL,Spl,anon-WO0142479.5,l(2)05091,spl,sply
General protein information	Preferred Names Sphingosine-1-phosphate lyase Names CG8946 gene product from transcript CG8946-RA CG8946-PA CG8946-PB Sply-PA Sply-PB sphinganine-1-phosphate lyase sphingosine-1-phosphate lyase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53F6-53F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_166215.2	NP_725652.1	Sphingosine-1-phosphate lyase, isoform B
NM_143775.3	NP_652032.1	Sphingosine-1-phosphate lyase, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SGPL1	XP_001171432.1
	SGPL1	XP_001106861.2
Saccharomyces cerevisiae (baker's yeast)	DPL1	NP_010580.1
Oryza sativa (rice)	Os01g0100900	NP_001041740.2
Canis lupus familiaris (dog)	SGPL1	XP_546150.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001724	XP_321361.5
Caenorhabditis elegans (roundworm)	tag-38	NP_505372.1
Magnaporthe oryzae (rice blast fungus)	MGG_07162	XP_003715360.1
Xenopus tropicalis (tropical clawed frog)	sgpl1	XP_002943540.2
Bos taurus (cattle)	SGPL1	NP_001091522.1
Homo sapiens (human)	SGPL1	NP_003892.2
Mus musculus (house mouse)	Sgpl1	NP_033189.2
Rattus norvegicus (Norway rat)	Sgpl1	NP_775139.1
Gallus gallus (chicken)	SGPL1	NP_001007947.1
Danio rerio (zebrafish)	sgpl1	NP_001082938.1
Drosophila melanogaster (fruit fly)	Sply	NP_725652.1
Kluyveromyces lactis	KLLA0C10505g	XP_452668.1
Neurospora crassa	NCU06761	XP_963533.1
Arabidopsis thaliana (thale cress)	DPL1	NP_174119.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Drosophila, Sply mutants, which lack SGPL1, displayed a phenotype reminiscent of nephrotic syndrome in nephrocytes.
Title: Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.

There are defects in reproductive structures and function that are associated with enhanced apoptosis in Drosophila Sply05091 mutants that lack functional S1P lyase and thereby accumulate sphingolipid long chain base metabolites.
Title: Disruption of sphingolipid metabolism elicits apoptosis-associated reproductive defects in Drosophila.

cloning of the sphingosine-1-phosphate lyase gene (Sply) and its importance for adult muscle development and integrity, reproduction and larval viability
Title: Sply regulation of sphingolipid signaling molecules is essential for Drosophila development.

The following Sply gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sply cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12733	NM_166215.2 Latest version!	Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12733	NM_143775.3 Latest version!	Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sply ( NM_166215.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725652.1 Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12733
Clone ID Related Accession (Same CDS sequence)	NM_166215.2 , NM_143775.3
Accession Version	NM_166215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sphingosine-1-phosphate lyase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166215.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCCGT TCTCCGGCAG CGATTGCCTT AAGCCCGTCA CCGAGGGCAT CAACCGGGCG 
TTCGGCGCCA AGGAGCCCTG GCAGGTGGCC ACCATCACGG CCACCACGGT GCTGGGAGGC 
GTCTGGCTCT GGACTGTGAT CTGCCAGGAT GAAAATCTTT ACATTCGTGG CAAGCGTCAG 
TTCTTTAAGT TTGCCAAGAA GATTCCAGCC GTGCGTCGTC AGGTGGAGAC TGAATTGGCC 
AAGGCCAAAA ACGACTTCGA GACGGAAATC AAAAAGAGCA ACGCCCACCT TACCTACTCG 
GAAACTCTGC CCGAGAAGGG ACTCAGCAAG GAGGAGATCC TCCGACTGGT GGATGAGCAC 
CTGAAGACTG GTCACTACAA CTGGCGTGAT GGTCGTGTAT CTGGCGCGGT CTACGGCTAC 
AAGCCTGATC TGGTGGAGCT CGTCACTGAA GTGTACGGCA AGGCCTCCTA CACCAATCCC 
TTGCACGCAG ATCTTTTCCC GGGAGTTTGC AAAATGGAGG CGGAGGTAGT GCGCATGGCA 
TGCAACCTGT TCCATGGAAA CTCAGCCAGC TGTGGAACCA TGACCACCGG CGGCACCGAA 
TCCATTGTAA TGGCCATGAA GGCGTACAGG GATTTCGCTA GAGAGTACAA GGGAATCACC 
AGGCCAAACA TCGTGGTGCC TAAGACGGTC CACGCGGCCT TCGACAAGGG CGGTCAGTAC 
TTTAATATCC ACGTGCGATC CGTGGATGTA GATCCGGAGA CCTACGAAGT GGACATTAAG 
AAGTTCAAAC GTGCCATTAA CAGGAACACG ATTCTGCTGG TTGGGTCTGC TCCGAACTTC 
CCCTATGGAA CCATCGATGA CATCGAAGCT ATCGCCGCTT TGGGCGTTAA GTACGACATT 
CCCGTGCACG TGGACGCCTG CCTGGGCAGC TTTGTGGTGG CCTTGGTCCG CAACGCCGGC 
TATAAGCTGC GTCCCTTCGA CTTTGAGGTC AAGGGAGTGA CCAGTATCTC CGCTGATACC 
CACAAGTATG GTTTCGCGCC CAAGGGATCA TCGGTGATCC TTTACTCGGA CAAGAAGTAC 
AAGGACCATC AGTTCACTGT GACTACTGAC TGGCCTGGCG GCGTGTATGG TTCTCCCACA 
GTCAACGGTT CCCGTGCCGG AGGTATTATC GCCGCCTGCT GGGCTACCAT GATGAGCTTT 
GGCTATGATG GTTATCTGGA AGCCACTAAG CGCATTGTGG ATACGGCGCG CTATATCGAG 
AGGGGCGTTC GCGACATCGA TGGCATCTTT ATCTTTGGCA AGCCAGCTAC TTCAGTGATT 
GCCCTGGGTT CCAATGTGTT TGACATTTTC CGGCTATCGG ATTCGCTGTG CAAACTGGGC 
TGGAACCTGA ATGCGCTGCA GTTTCCATCT GGTATCCACC TGTGCGTGAC GGACATGCAC 
ACACAGCCCG GAGTCGCGGA TAAATTCATT GCCGATGTGC GCAGCTGTAC GGCGGAGATC 
ATGAAGGATC CCGGCCAGCC CGTCGTTGGA AAGATGGCTC TCTACGGCAT GGCACAGAGC 
ATACCCGACC GTTCGGTGAT CGGAGAAGTG ACTCGCCTAT TCCTGCACTC CATGTACTAC 
ACTCCCAGCC AGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725652.1
CDS	98..1735
Translation	MRPFSGSDCLKPVTEGINRAFGAKEPWQVATITATTVLGGVWLWTVICQDENLYIRGKRQFFKFAKKIPAVRRQVETELAKAKNDFETEIKKSNAHLTYSETLPEKGLSKEEILRLVDEHLKTGHYNWRDGRVSGAVYGYKPDLVELVTEVYGKASYTNPLHADLFPGVCKMEAEVVRMACNLFHGNSASCGTMTTGGTESIVMAMKAYRDFAREYKGITRPNIVVPKTVHAAFDKGGQYFNIHVRSVDVDPETYEVDIKKFKRAINRNTILLVGSAPNFPYGTIDDIEAIAALGVKYDIPVHVDACLGSFVVALVRNAGYKLRPFDFEVKGVTSISADTHKYGFAPKGSSVILYSDKKYKDHQFTVTTDWPGGVYGSPTVNGSRAGGIIAACWATMMSFGYDGYLEATKRIVDTARYIERGVRDIDGIFIFGKPATSVIALGSNVFDIFRLSDSLCKLGWNLNALQFPSGIHLCVTDMHTQPGVADKFIADVRSCTAEIMKDPGQPVVGKMALYGMAQSIPDRSVIGEVTRLFLHSMYYTPSQK

Target ORF information:

RefSeq Version	NM_166215.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166215.2

ORF Insert Sequence:

ATGCGTCCGT TCTCCGGCAG CGATTGCCTT AAGCCCGTCA CCGAGGGCAT CAACCGGGCG 
TTCGGCGCCA AGGAGCCCTG GCAGGTGGCC ACCATCACGG CCACCACGGT GCTGGGAGGC 
GTCTGGCTCT GGACTGTGAT CTGCCAGGAT GAAAATCTTT ACATTCGTGG CAAGCGTCAG 
TTCTTTAAGT TTGCCAAGAA GATTCCAGCC GTGCGTCGTC AGGTGGAGAC TGAATTGGCC 
AAGGCCAAAA ACGACTTCGA GACGGAAATC AAAAAGAGCA ACGCCCACCT TACCTACTCG 
GAAACTCTGC CCGAGAAGGG ACTCAGCAAG GAGGAGATCC TCCGACTGGT GGATGAGCAC 
CTGAAGACTG GTCACTACAA CTGGCGTGAT GGTCGTGTAT CTGGCGCGGT CTACGGCTAC 
AAGCCTGATC TGGTGGAGCT CGTCACTGAA GTGTACGGCA AGGCCTCCTA CACCAATCCC 
TTGCACGCAG ATCTTTTCCC GGGAGTTTGC AAAATGGAGG CGGAGGTAGT GCGCATGGCA 
TGCAACCTGT TCCATGGAAA CTCAGCCAGC TGTGGAACCA TGACCACCGG CGGCACCGAA 
TCCATTGTAA TGGCCATGAA GGCGTACAGG GATTTCGCTA GAGAGTACAA GGGAATCACC 
AGGCCAAACA TCGTGGTGCC TAAGACGGTC CACGCGGCCT TCGACAAGGG CGGTCAGTAC 
TTTAATATCC ACGTGCGATC CGTGGATGTA GATCCGGAGA CCTACGAAGT GGACATTAAG 
AAGTTCAAAC GTGCCATTAA CAGGAACACG ATTCTGCTGG TTGGGTCTGC TCCGAACTTC 
CCCTATGGAA CCATCGATGA CATCGAAGCT ATCGCCGCTT TGGGCGTTAA GTACGACATT 
CCCGTGCACG TGGACGCCTG CCTGGGCAGC TTTGTGGTGG CCTTGGTCCG CAACGCCGGC 
TATAAGCTGC GTCCCTTCGA CTTTGAGGTC AAGGGAGTGA CCAGTATCTC CGCTGATACC 
CACAAGTATG GTTTCGCGCC CAAGGGATCA TCGGTGATCC TTTACTCGGA CAAGAAGTAC 
AAGGACCATC AGTTCACTGT GACTACTGAC TGGCCTGGCG GCGTGTATGG TTCTCCCACA 
GTCAACGGTT CCCGTGCCGG AGGTATTATC GCCGCCTGCT GGGCTACCAT GATGAGCTTT 
GGCTATGATG GTTATCTGGA AGCCACTAAG CGCATTGTGG ATACGGCGCG CTATATCGAG 
AGGGGCGTTC GCGACATCGA TGGCATCTTT ATCTTTGGCA AGCCAGCTAC TTCAGTGATT 
GCCCTGGGTT CCAATGTGTT TGACATTTTC CGGCTATCGG ATTCGCTGTG CAAACTGGGC 
TGGAACCTGA ATGCGCTGCA GTTTCCATCT GGTATCCACC TGTGCGTGAC GGACATGCAC 
ACACAGCCCG GAGTCGCGGA TAAATTCATT GCCGATGTGC GCAGCTGTAC GGCGGAGATC 
ATGAAGGATC CCGGCCAGCC CGTCGTTGGA AAGATGGCTC TCTACGGCAT GGCACAGAGC 
ATACCCGACC GTTCGGTGAT CGGAGAAGTG ACTCGCCTAT TCCTGCACTC CATGTACTAC 
ACTCCCAGCC AGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sply ( NM_143775.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652032.1 Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12733
Clone ID Related Accession (Same CDS sequence)	NM_166215.2 , NM_143775.3
Accession Version	NM_143775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sphingosine-1-phosphate lyase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_143775.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCCGT TCTCCGGCAG CGATTGCCTT AAGCCCGTCA CCGAGGGCAT CAACCGGGCG 
TTCGGCGCCA AGGAGCCCTG GCAGGTGGCC ACCATCACGG CCACCACGGT GCTGGGAGGC 
GTCTGGCTCT GGACTGTGAT CTGCCAGGAT GAAAATCTTT ACATTCGTGG CAAGCGTCAG 
TTCTTTAAGT TTGCCAAGAA GATTCCAGCC GTGCGTCGTC AGGTGGAGAC TGAATTGGCC 
AAGGCCAAAA ACGACTTCGA GACGGAAATC AAAAAGAGCA ACGCCCACCT TACCTACTCG 
GAAACTCTGC CCGAGAAGGG ACTCAGCAAG GAGGAGATCC TCCGACTGGT GGATGAGCAC 
CTGAAGACTG GTCACTACAA CTGGCGTGAT GGTCGTGTAT CTGGCGCGGT CTACGGCTAC 
AAGCCTGATC TGGTGGAGCT CGTCACTGAA GTGTACGGCA AGGCCTCCTA CACCAATCCC 
TTGCACGCAG ATCTTTTCCC GGGAGTTTGC AAAATGGAGG CGGAGGTAGT GCGCATGGCA 
TGCAACCTGT TCCATGGAAA CTCAGCCAGC TGTGGAACCA TGACCACCGG CGGCACCGAA 
TCCATTGTAA TGGCCATGAA GGCGTACAGG GATTTCGCTA GAGAGTACAA GGGAATCACC 
AGGCCAAACA TCGTGGTGCC TAAGACGGTC CACGCGGCCT TCGACAAGGG CGGTCAGTAC 
TTTAATATCC ACGTGCGATC CGTGGATGTA GATCCGGAGA CCTACGAAGT GGACATTAAG 
AAGTTCAAAC GTGCCATTAA CAGGAACACG ATTCTGCTGG TTGGGTCTGC TCCGAACTTC 
CCCTATGGAA CCATCGATGA CATCGAAGCT ATCGCCGCTT TGGGCGTTAA GTACGACATT 
CCCGTGCACG TGGACGCCTG CCTGGGCAGC TTTGTGGTGG CCTTGGTCCG CAACGCCGGC 
TATAAGCTGC GTCCCTTCGA CTTTGAGGTC AAGGGAGTGA CCAGTATCTC CGCTGATACC 
CACAAGTATG GTTTCGCGCC CAAGGGATCA TCGGTGATCC TTTACTCGGA CAAGAAGTAC 
AAGGACCATC AGTTCACTGT GACTACTGAC TGGCCTGGCG GCGTGTATGG TTCTCCCACA 
GTCAACGGTT CCCGTGCCGG AGGTATTATC GCCGCCTGCT GGGCTACCAT GATGAGCTTT 
GGCTATGATG GTTATCTGGA AGCCACTAAG CGCATTGTGG ATACGGCGCG CTATATCGAG 
AGGGGCGTTC GCGACATCGA TGGCATCTTT ATCTTTGGCA AGCCAGCTAC TTCAGTGATT 
GCCCTGGGTT CCAATGTGTT TGACATTTTC CGGCTATCGG ATTCGCTGTG CAAACTGGGC 
TGGAACCTGA ATGCGCTGCA GTTTCCATCT GGTATCCACC TGTGCGTGAC GGACATGCAC 
ACACAGCCCG GAGTCGCGGA TAAATTCATT GCCGATGTGC GCAGCTGTAC GGCGGAGATC 
ATGAAGGATC CCGGCCAGCC CGTCGTTGGA AAGATGGCTC TCTACGGCAT GGCACAGAGC 
ATACCCGACC GTTCGGTGAT CGGAGAAGTG ACTCGCCTAT TCCTGCACTC CATGTACTAC 
ACTCCCAGCC AGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652032.1
CDS	161..1798
Translation	MRPFSGSDCLKPVTEGINRAFGAKEPWQVATITATTVLGGVWLWTVICQDENLYIRGKRQFFKFAKKIPAVRRQVETELAKAKNDFETEIKKSNAHLTYSETLPEKGLSKEEILRLVDEHLKTGHYNWRDGRVSGAVYGYKPDLVELVTEVYGKASYTNPLHADLFPGVCKMEAEVVRMACNLFHGNSASCGTMTTGGTESIVMAMKAYRDFAREYKGITRPNIVVPKTVHAAFDKGGQYFNIHVRSVDVDPETYEVDIKKFKRAINRNTILLVGSAPNFPYGTIDDIEAIAALGVKYDIPVHVDACLGSFVVALVRNAGYKLRPFDFEVKGVTSISADTHKYGFAPKGSSVILYSDKKYKDHQFTVTTDWPGGVYGSPTVNGSRAGGIIAACWATMMSFGYDGYLEATKRIVDTARYIERGVRDIDGIFIFGKPATSVIALGSNVFDIFRLSDSLCKLGWNLNALQFPSGIHLCVTDMHTQPGVADKFIADVRSCTAEIMKDPGQPVVGKMALYGMAQSIPDRSVIGEVTRLFLHSMYYTPSQK

Target ORF information:

RefSeq Version	NM_143775.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143775.3

ORF Insert Sequence:

ATGCGTCCGT TCTCCGGCAG CGATTGCCTT AAGCCCGTCA CCGAGGGCAT CAACCGGGCG 
TTCGGCGCCA AGGAGCCCTG GCAGGTGGCC ACCATCACGG CCACCACGGT GCTGGGAGGC 
GTCTGGCTCT GGACTGTGAT CTGCCAGGAT GAAAATCTTT ACATTCGTGG CAAGCGTCAG 
TTCTTTAAGT TTGCCAAGAA GATTCCAGCC GTGCGTCGTC AGGTGGAGAC TGAATTGGCC 
AAGGCCAAAA ACGACTTCGA GACGGAAATC AAAAAGAGCA ACGCCCACCT TACCTACTCG 
GAAACTCTGC CCGAGAAGGG ACTCAGCAAG GAGGAGATCC TCCGACTGGT GGATGAGCAC 
CTGAAGACTG GTCACTACAA CTGGCGTGAT GGTCGTGTAT CTGGCGCGGT CTACGGCTAC 
AAGCCTGATC TGGTGGAGCT CGTCACTGAA GTGTACGGCA AGGCCTCCTA CACCAATCCC 
TTGCACGCAG ATCTTTTCCC GGGAGTTTGC AAAATGGAGG CGGAGGTAGT GCGCATGGCA 
TGCAACCTGT TCCATGGAAA CTCAGCCAGC TGTGGAACCA TGACCACCGG CGGCACCGAA 
TCCATTGTAA TGGCCATGAA GGCGTACAGG GATTTCGCTA GAGAGTACAA GGGAATCACC 
AGGCCAAACA TCGTGGTGCC TAAGACGGTC CACGCGGCCT TCGACAAGGG CGGTCAGTAC 
TTTAATATCC ACGTGCGATC CGTGGATGTA GATCCGGAGA CCTACGAAGT GGACATTAAG 
AAGTTCAAAC GTGCCATTAA CAGGAACACG ATTCTGCTGG TTGGGTCTGC TCCGAACTTC 
CCCTATGGAA CCATCGATGA CATCGAAGCT ATCGCCGCTT TGGGCGTTAA GTACGACATT 
CCCGTGCACG TGGACGCCTG CCTGGGCAGC TTTGTGGTGG CCTTGGTCCG CAACGCCGGC 
TATAAGCTGC GTCCCTTCGA CTTTGAGGTC AAGGGAGTGA CCAGTATCTC CGCTGATACC 
CACAAGTATG GTTTCGCGCC CAAGGGATCA TCGGTGATCC TTTACTCGGA CAAGAAGTAC 
AAGGACCATC AGTTCACTGT GACTACTGAC TGGCCTGGCG GCGTGTATGG TTCTCCCACA 
GTCAACGGTT CCCGTGCCGG AGGTATTATC GCCGCCTGCT GGGCTACCAT GATGAGCTTT 
GGCTATGATG GTTATCTGGA AGCCACTAAG CGCATTGTGG ATACGGCGCG CTATATCGAG 
AGGGGCGTTC GCGACATCGA TGGCATCTTT ATCTTTGGCA AGCCAGCTAC TTCAGTGATT 
GCCCTGGGTT CCAATGTGTT TGACATTTTC CGGCTATCGG ATTCGCTGTG CAAACTGGGC 
TGGAACCTGA ATGCGCTGCA GTTTCCATCT GGTATCCACC TGTGCGTGAC GGACATGCAC 
ACACAGCCCG GAGTCGCGGA TAAATTCATT GCCGATGTGC GCAGCTGTAC GGCGGAGATC 
ATGAAGGATC CCGGCCAGCC CGTCGTTGGA AAGATGGCTC TCTACGGCAT GGCACAGAGC 
ATACCCGACC GTTCGGTGAT CGGAGAAGTG ACTCGCCTAT TCCTGCACTC CATGTACTAC 
ACTCCCAGCC AGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sply cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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