Sgpl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sgpl1
Entrez Gene ID	286896
Full Name	sphingosine-1-phosphate lyase 1
Synonyms	S1PL,Spgl1,spl
General protein information	Preferred Names sphingosine-1-phosphate lyase 1 Names sphingosine-1-phosphate lyase 1 SP-lyase 1 SPL 1 sphingosine phosphate lyase 1 sphingosine-1-phosphate aldolase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	catalyzes the conversion of phosphorylated long chain bases into ethanolamine phosphate and a fatty aldehyde in sphingolipid catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772871.1	XP_008771093.1	sphingosine-1-phosphate lyase 1 isoform X1
XM_008772872.1	XP_008771094.1	sphingosine-1-phosphate lyase 1 isoform X1
XM_008772873.1	XP_008771095.1	sphingosine-1-phosphate lyase 1 isoform X1
NM_173116.1	NP_775139.1	sphingosine-1-phosphate lyase 1
XM_008772870.2	XP_008771092.1	sphingosine-1-phosphate lyase 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SGPL1	XP_001171432.1
	SGPL1	XP_001106861.2
Saccharomyces cerevisiae (baker's yeast)	DPL1	NP_010580.1
Oryza sativa (rice)	Os01g0100900	NP_001041740.2
Canis lupus familiaris (dog)	SGPL1	XP_546150.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001724	XP_321361.5
Caenorhabditis elegans (roundworm)	tag-38	NP_505372.1
Magnaporthe oryzae (rice blast fungus)	MGG_07162	XP_003715360.1
Xenopus tropicalis (tropical clawed frog)	sgpl1	XP_002943540.2
Bos taurus (cattle)	SGPL1	NP_001091522.1
Homo sapiens (human)	SGPL1	NP_003892.2
Mus musculus (house mouse)	Sgpl1	NP_033189.2
Rattus norvegicus (Norway rat)	Sgpl1	NP_775139.1
Gallus gallus (chicken)	SGPL1	NP_001007947.1
Danio rerio (zebrafish)	sgpl1	NP_001082938.1
Drosophila melanogaster (fruit fly)	Sply	NP_725652.1
Kluyveromyces lactis	KLLA0C10505g	XP_452668.1
Neurospora crassa	NCU06761	XP_963533.1
Arabidopsis thaliana (thale cress)	DPL1	NP_174119.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of sphingosine-1-phosphate lyase protects insulin-secreting cells against cytokine toxicity.
Hahn C, Tyka K, Saba JD, Lenzen S, Gurgul-Convey E
The Journal of biological chemistry292(49)20292-20304(2017 12)

Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.
Lovric S, Goncalves S, Gee HY, Oskouian B, Srinivas H, Choi WI, Shril S, Ashraf S, Tan W, Rao J, Airik M, Schapiro D, Braun DA, Sadowski CE, Widmeier E, Jobst-Schwan T, Schmidt JM, Girik V, Capitani G, Suh JH, Lachauss?e N, Arrondel C, Patat J, Gribouval O, Furlano M, Boyer O, Schmitt A, Vuiblet V, Hashmi S, Wilcken R, Bernier FP, Innes AM, Parboosingh JS, Lamont RE, Midgley JP, Wright N, Majewski J, Zenker M, Schaefer F, Kuss N, Greil J, Giese T, Schwarz K, Catheline V, Schanze D, Franke I, Sznajer Y, Truant AS, Adams B, D?sir J, Biemann R, Pei Y, Ars E, Lloberas N, Madrid A, Dharnidharka VR, Connolly AM, Willing MC, Cooper MA, Lifton RP, Simons M, Riezman H, Antignac C, Saba JD, Hildebrandt F
The Journal of clinical investigation127(3)912-928(2017 Mar)

Reduced Activity of Sphingosine-1-Phosphate Lyase Induces Podocyte-related Glomerular Proteinuria, Skin Irritation, and Platelet Activation.
Sch?mann J, Grevot A, Ledieu D, Wolf A, Schubart A, Piaia A, Sutter E, C?t? S, Beerli C, Pognan F, Billich A, Moulin P, Walker UJ
Toxicologic pathology43(5)694-703(2015 Jul)

Orally active 7-substituted (4-benzylphthalazin-1-yl)-2-methylpiperazin-1-yl]nicotinonitriles as active-site inhibitors of sphingosine 1-phosphate lyase for the treatment of multiple sclerosis.
Weiler S, Braendlin N, Beerli C, Bergsdorf C, Schubart A, Srinivas H, Oberhauser B, Billich A
Journal of medicinal chemistry57(12)5074-84(2014 Jun)

The PDGF signaling pathway controls multiple steroid-producing lineages.
Schmahl J, Rizzolo K, Soriano P
Genes & development22(23)3255-67(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the relatively low endogenous Spl expression level in insulin-secreting cells contributes to their extraordinary vulnerability to proinflammatory cytokine toxicity
Title: Overexpression of sphingosine-1-phosphate lyase protects insulin-secreting cells against cytokine toxicity.

Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency
Title: Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.

Studied pathological alterations associated with only partially reduced activity of S1P lyase (as may be encountered upon pharmacological inhibition).
Title: Reduced Activity of Sphingosine-1-Phosphate Lyase Induces Podocyte-related Glomerular Proteinuria, Skin Irritation, and Platelet Activation.

The following Sgpl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sgpl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03443	NM_173116.1 Latest version!	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa03443	XM_008772873.1 Latest version!	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa03443	XM_008772870.2 Latest version!	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa03443	XM_008772871.1 Latest version!	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa03443	XM_008772872.1 Latest version!	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sgpl1 ( NM_173116.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775139.1 Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), mRNA.

Price & Availability↑

CloneID	ORa03443
Clone ID Related Accession (Same CDS sequence)	XM_008772871.1 , XM_008772872.1 , XM_008772873.1 , NM_173116.1 , XM_008772870.2
Accession Version	NM_173116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine-1-phosphate lyase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ512838.1. ##Evidence-Data-START## CDS exon combination :: AJ512838.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775139.1
CDS	130..1836
Translation	MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRFKNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQMNGSPKPR

Target ORF information:

RefSeq Version	NM_173116.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173116.1

ORF Insert Sequence:

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgpl1 ( XM_008772871.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771093.1 Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa03443
Clone ID Related Accession (Same CDS sequence)	XM_008772871.1 , XM_008772872.1 , XM_008772873.1 , NM_173116.1 , XM_008772870.2
Accession Version	XM_008772871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine-1-phosphate lyase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771093.1
CDS	79..1785
Translation	MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRFKNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQMNGSPKPR

Target ORF information:

RefSeq Version	XM_008772871.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772871.1

ORF Insert Sequence:

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgpl1 ( XM_008772872.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771094.1 Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa03443
Clone ID Related Accession (Same CDS sequence)	XM_008772871.1 , XM_008772872.1 , XM_008772873.1 , NM_173116.1 , XM_008772870.2
Accession Version	XM_008772872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine-1-phosphate lyase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771094.1
CDS	50..1756
Translation	MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRFKNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQMNGSPKPR

Target ORF information:

RefSeq Version	XM_008772872.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772872.1

ORF Insert Sequence:

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgpl1 ( XM_008772873.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771095.1 Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X4, mRNA.

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CloneID	ORa03443
Clone ID Related Accession (Same CDS sequence)	XM_008772871.1 , XM_008772872.1 , XM_008772873.1 , NM_173116.1 , XM_008772870.2
Accession Version	XM_008772873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine-1-phosphate lyase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771095.1
CDS	137..1843
Translation	MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRFKNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQMNGSPKPR

Target ORF information:

RefSeq Version	XM_008772873.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772873.1

ORF Insert Sequence:

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgpl1 ( XM_008772870.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771092.1 Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X1, mRNA.

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CloneID	ORa03443
Clone ID Related Accession (Same CDS sequence)	XM_008772871.1 , XM_008772872.1 , XM_008772873.1 , NM_173116.1 , XM_008772870.2
Accession Version	XM_008772870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine-1-phosphate lyase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771092.1
CDS	68..1774
Translation	MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRFKNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQMNGSPKPR

Target ORF information:

RefSeq Version	XM_008772870.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine-1-phosphate lyase 1 (Sgpl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772870.2

ORF Insert Sequence:

ATGCCGAGTA CCGACCTTCT CAAGTTGAAG GACTTCGAGC CTTATTTGGA GATTTTGGAA 
GCATATTCCA CAAAAGCCAA GAATTACGTG AATGGGTACT GCACCAAATA CGAGCCCTGG 
CAGCTCATTG CGGGGAGTGT CCTGTGTACT CTGCTGGTAG TCTGGGTATA TGAGCTTATC 
TTCCAGCCAG AGAGTTTATG GTCTCGGTTT AAAAATAAAT TATTTAGGCT TATAAGGAAG 
ATGCCATTTA TTGGACGTAA GATACAACAG CAGCTTACCA AAGCCAAGAA GGATCTTGTC 
AAGAACATGC CATTCCTGAA GTTGGACAAA GATTATGTGA AAACCCTGCC TGCTCAGGGT 
CTGAGCACAG CTGAGGTTCT GGAGAGACTC AAGGAGTACA GCTCCATGGA TGTCTTCTGG 
CAAGAAGGGA AAGCCTCAGG AGCTGTGTAC AGTGGGGAGC CGAAGCTCAC CGAGCTGCTG 
GTGCAGGCTT ACGGAGAATT CACGTGGAGC AATCCACTGC ACCCAGATAT CTTCCCCGGA 
CTACGGAAGT TAGAGGCAGA AATCGTGAGG ATGACTTGCT CCCTCTTCAA TGGGGGGCCA 
GACTCCTGTG GATGTGTGAC TTCTGGGGGA ACAGAAAGCA TCCTAATGGC CTGCAAAGCA 
TATCGGGACT TGGCCTTAGA GAAGGGGATC AAAACTCCAG AAATTGTGGC TCCTGAGAGT 
GCCCACGCCG CATTCGACAA AGCAGCTCAT TATTTCGGAA TGAAGATTGT ACGCGTTGCG 
CAGAAAAAGA ACATGGAGGT GGATGTGCGG GCAATGAAAA GAGCCATCTC CAGGAACACA 
GCCATGCTGG TCTGTTCTGC CCCACAGTTT CCTCATGGTG TGATAGATCC TATCCCCGAA 
GTGGCCAAGC TGGCTGTCAA ATATAAAATC CCATTCCATG TGGATGCTTG TCTTGGGGGC 
TTCCTCATTG TCTTCATGGA GAAAGCAGGG TACCCACTGG AGAAACCATT TGATTTCCGG 
GTGAAAGGTG TGACCAGCAT TTCAGCAGAT ACTCATAAGT ACGGCTATGC TCCCAAAGGT 
TCATCAGTGG TGATGTACTC TAACGAGAAG TACAGGAAGT ACCAGTTCTT TGTTGATGCA 
GATTGGCAAG GTGGCATCTA CGCATCTCCA AGCATAGCTG GCTCACGGCC TGGTGGCATC 
ATTGCAGCCT GTTGGGCCGC CTTGATGCAC TTTGGTGAGA ACGGCTATGT TGAAGCTACC 
AAACAGATCA TCAAAACTGC TCGCTTCCTC AAGTCAGAAC TGGAAAACAT CAAAAACATC 
TTCATTTTGG GGGATCCTCA ATTGTCAGTT ATTGCTCTGG GCTCCAACGA TTTTGACATC 
TACCGACTAT CTAATATGAT GTCTGCTAAG GGGTGGAATT TTAACTACTT GCAGTTCCCA 
AGAAGCATTC ATTTCTGCAT TACGTTAGTA CATACTCGGA AGCGAGTGGC AATCCAGTTC 
CTAAAGGATA TCCGGGAATC GGTCACACAA ATCATGAAGA ATCCTAAAGC TAAGACCACA 
GGAATGGGTG CCATCTATGG CATGGCCCAG GCAACCATTG ACAGGAAGAT GGTTGCAGAA 
ATATCCTCCG TCTTCTTGGA CAGCCTTTAT AGTACAGACC CTGTGACTCA GGGTAACCAG 
ATGAACGGTT CTCCAAAGCC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of sphingosine-1-phosphate lyase protects insulin-secreting cells against cytokine toxicity.
The Journal of biological chemistry292(49)20292-20304(2017 12)
Hahn C,Tyka K,Saba JD,Lenzen S,Gurgul-Convey E

Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.
The Journal of clinical investigation127(3)912-928(2017 Mar)
Lovric S,Goncalves S,Gee HY,Oskouian B,Srinivas H,Choi WI,Shril S,Ashraf S,Tan W,Rao J,Airik M,Schapiro D,Braun DA,Sadowski CE,Widmeier E,Jobst-Schwan T,Schmidt JM,Girik V,Capitani G,Suh JH,Lachauss?e N,Arrondel C,Patat J,Gribouval O,Furlano M,Boyer O,Schmitt A,Vuiblet V,Hashmi S,Wilcken R,Bernier FP,Innes AM,Parboosingh JS,Lamont RE,Midgley JP,Wright N,Majewski J,Zenker M,Schaefer F,Kuss N,Greil J,Giese T,Schwarz K,Catheline V,Schanze D,Franke I,Sznajer Y,Truant AS,Adams B,D?sir J,Biemann R,Pei Y,Ars E,Lloberas N,Madrid A,Dharnidharka VR,Connolly AM,Willing MC,Cooper MA,Lifton RP,Simons M,Riezman H,Antignac C,Saba JD,Hildebrandt F

Reduced Activity of Sphingosine-1-Phosphate Lyase Induces Podocyte-related Glomerular Proteinuria, Skin Irritation, and Platelet Activation.
Toxicologic pathology43(5)694-703(2015 Jul)
Sch?mann J,Grevot A,Ledieu D,Wolf A,Schubart A,Piaia A,Sutter E,C?t? S,Beerli C,Pognan F,Billich A,Moulin P,Walker UJ

Orally active 7-substituted (4-benzylphthalazin-1-yl)-2-methylpiperazin-1-yl]nicotinonitriles as active-site inhibitors of sphingosine 1-phosphate lyase for the treatment of multiple sclerosis.
Journal of medicinal chemistry57(12)5074-84(2014 Jun)
Weiler S,Braendlin N,Beerli C,Bergsdorf C,Schubart A,Srinivas H,Oberhauser B,Billich A

The PDGF signaling pathway controls multiple steroid-producing lineages.
Genes & development22(23)3255-67(2008 Dec)
Schmahl J,Rizzolo K,Soriano P

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Sgpl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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