PTGIS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTGIS
Entrez Gene ID	282021
Full Name	prostaglandin I2 synthase
General protein information	Preferred Names prostaglandin I2 synthase Names prostacyclin synthase prostaglandin I2 (prostacyclin) synthase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174444.1	NP_776869.1	prostacyclin synthase precursor
XM_015474114.1	XP_015329600.1	prostacyclin synthase isoform X1
XM_015474115.1	XP_015329601.1	prostacyclin synthase isoform X2
XM_015474115.2	XP_015329601.2	prostacyclin synthase isoform X1
XM_015474114.2	XP_015329600.1	prostacyclin synthase isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PTGIS	XP_005635412.1
Mus musculus (house mouse)	Ptgis	NP_032994.1
Pan troglodytes (chimpanzee)	PTGIS	XP_514713.3
Rattus norvegicus (Norway rat)	Ptgis	NP_113745.1
Xenopus tropicalis (tropical clawed frog)	ptgis	XP_002933228.2
Homo sapiens (human)	PTGIS	NP_000952.1
	PTGIS	XP_001098575.1
Bos taurus (cattle)	PTGIS	NP_776869.1
Danio rerio (zebrafish)	ptgis	NP_001104630.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Specific nitration at tyrosine 430 revealed by high resolution mass spectrometry as basis for redox regulation of bovine prostacyclin synthase.
Schmidt P, Youhnovski N, Daiber A, Balan A, Arsic M, Bachschmid M, Przybylski M, Ullrich V
The Journal of biological chemistry278(15)12813-9(2003 Apr)

Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.
Hara S, Miyata A, Yokoyama C, Inoue H, Brugger R, Lottspeich F, Ullrich V, Tanabe T
The Journal of biological chemistry269(31)19897-903(1994 Aug)

Molecular cloning and characterization of bovine prostacyclin synthase.
Pereira B, Wu KK, Wang LH
Biochemical and biophysical research communications203(1)59-66(1994 Aug)

Bovine prostacyclin synthase: purification and isolation of partial cDNA.
Pereira B, Wu KK, Wang LH
Biochemical and biophysical research communications197(3)1041-8(1993 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Specific nitration at tyrosine 430 is revealed by high resolution mass spectrometry as basis for redox regulation of this enzyme.
Title: Specific nitration at tyrosine 430 revealed by high resolution mass spectrometry as basis for redox regulation of bovine prostacyclin synthase.

The following PTGIS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTGIS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42413	NM_174444.1 Latest version!	Bos taurus prostaglandin I2 synthase (PTGIS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa221447	XM_015474114.2 Latest version!	Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa92296	XM_015474114.1 Latest version!	Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa92297	XM_015474115.1 Latest version!	Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa221446	XM_015474115.2 Latest version!	Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTGIS ( NM_174444.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776869.1 Bos taurus prostaglandin I2 synthase (PTGIS), mRNA.

Price & Availability↑

CloneID	OBa42413
Clone ID Related Accession (Same CDS sequence)	NM_174444.1
Accession Version	NM_174444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	prostacyclin synthase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D30718.1. On Mar 29, 2005 this sequence version replaced XM_613497.1. Sequence Note: This sequence has been modified as follows: removed 26 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: D30718.1, BC120087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145436 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTGGG CCGTGGTCTT CGGCCTCCTG GCCGCGCTGC TGCTGCTGCT GCTGCTGACC 
CGCCGCCGCA CGCGGCGACC TGGCGAGCCT CCCCTGGACC TGGGCAGTAT CCCCTGGCTG 
GGTCACGCCT TGGAGTTTGG AAAAGATGCT GCCGGCTTCC TCACTAGGAT GAAGGAGAAG 
CATGGGGACA TCTTTACTGT GCTGGTGGGC GGCAGGCACG TCACGGTCCT CCTGGATCCC 
CACTCCTACG ACGCGGTGGT GTGGGAGCCC CGCAGCAGGC TGGACTTCCA CGCCTACGCC 
GTCTTCCTCA TGGAGAGGAT TTTCGACGTG CAGCTTCCGC ATTATAACCC CGGTGATGAA 
AAGTCCAAGA TGAAACCGAC TCTCCTCCAC AAAGAGCTGC AGGCGCTCAC GGATGCCATG 
TATACCAACC TCCGCACTGT CCTGCTGGGT GACACCGTGG AAGCGGGCAG TGGCTGGCAC 
GAAATGGGGC TCCTCGAGTT CTCCTACGGC TTCCTGCTCA GAGCCGGCTA CCTGACCCAG 
TACGGCGTTG AGGCACCGCC ACACACCCAG GAGAGCCAGG CCCAGGACCG TGTCCACTCA 
GCCGACGTCT TCCACACCTT CCGCCAGCTT GACCTGCTGC TCCCCAAGCT GGCACGTGGC 
TCCCTGTCAG CGGGGGATAA GGACCGGGTG GGCAAGGTCA AAGGTCGCCT GTGGAAGCTG 
TTGTCCCCAA CCAGGCTGGC CAGCCGGGCC CACCGGAGCA GATGGCTGGA GAGTTACCTG 
CTGCACCTGG AGGAGATGGG CGTGTCGGAG GAGATGCAGG CTCGCGCCCT GGTGCTGCAG 
CTCTGGGCCA CACAGGGGAA CATGGGTCCT GCTGCCTTCT GGCTCCTGCT CTTTCTCCTC 
AAGAACCCCG AGGCCCTGGC CGCTGTCCGT GGAGAGCTAG AGACTGTCCT TTTGGGAGCA 
GAGCAGCCCA TATCACAGAT GACCACCCTC CCACAGAAAG TTTTGGACAG CATGCCTGTG 
CTGGACAGCG TGCTGAGTGA GAGCCTCAGG CTCACCGCCG CGCCCTTCAT CACCCGCGAG 
GTCGTGGCCG ACCTGGCCTT GCCCATGGCG GATGGGCGGG AATTCAGCCT GCGACGTGGG 
GACCGCCTCC TCCTCTTTCC CTTCCTGAGT CCCCAGAAGG ACCCGGAGAT CTACACAGAC 
CCGGAGGTAT TTAAATACAA CCGATTCCTG AACCCTGATG GATCAGAGAA GAAAGACTTT 
TACAAAGATG GGAAGCGACT GAAGAACTAC AGCTTGCCGT GGGGAGCAGG GCATAACCAG 
TGCCTGGGGA AGGGTTACGC CGTCAACAGC ATCAAACAAT TCGTGTTCCT TGTGCTGACG 
CAGTTTGACC TGGAGCTGAT CACCCCGGAC GTGGACATCC CCGAGTTTGA CCTCAGCAGG 
TATGGCTTTG GGCTGATGCA GCCGGAACAC GATGTGCCTG TCCGTTACCG CATCCGGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776869.1
CDS	22..1524
Translation	MSWAVVFGLLAALLLLLLLTRRRTRRPGEPPLDLGSIPWLGHALEFGKDAAGFLTRMKEKHGDIFTVLVGGRHVTVLLDPHSYDAVVWEPRSRLDFHAYAVFLMERIFDVQLPHYNPGDEKSKMKPTLLHKELQALTDAMYTNLRTVLLGDTVEAGSGWHEMGLLEFSYGFLLRAGYLTQYGVEAPPHTQESQAQDRVHSADVFHTFRQLDLLLPKLARGSLSAGDKDRVGKVKGRLWKLLSPTRLASRAHRSRWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELETVLLGAEQPISQMTTLPQKVLDSMPVLDSVLSESLRLTAAPFITREVVADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYSLPWGAGHNQCLGKGYAVNSIKQFVFLVLTQFDLELITPDVDIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Target ORF information:

RefSeq Version	NM_174444.1
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin I2 synthase (PTGIS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174444.1

ORF Insert Sequence:

ATGTCCTGGG CCGTGGTCTT CGGCCTCCTG GCCGCGCTGC TGCTGCTGCT GCTGCTGACC 
CGCCGCCGCA CGCGGCGACC TGGCGAGCCT CCCCTGGACC TGGGCAGTAT CCCCTGGCTG 
GGTCACGCCT TGGAGTTTGG AAAAGATGCT GCCGGCTTCC TCACTAGGAT GAAGGAGAAG 
CATGGGGACA TCTTTACTGT GCTGGTGGGC GGCAGGCACG TCACGGTCCT CCTGGATCCC 
CACTCCTACG ACGCGGTGGT GTGGGAGCCC CGCAGCAGGC TGGACTTCCA CGCCTACGCC 
GTCTTCCTCA TGGAGAGGAT TTTCGACGTG CAGCTTCCGC ATTATAACCC CGGTGATGAA 
AAGTCCAAGA TGAAACCGAC TCTCCTCCAC AAAGAGCTGC AGGCGCTCAC GGATGCCATG 
TATACCAACC TCCGCACTGT CCTGCTGGGT GACACCGTGG AAGCGGGCAG TGGCTGGCAC 
GAAATGGGGC TCCTCGAGTT CTCCTACGGC TTCCTGCTCA GAGCCGGCTA CCTGACCCAG 
TACGGCGTTG AGGCACCGCC ACACACCCAG GAGAGCCAGG CCCAGGACCG TGTCCACTCA 
GCCGACGTCT TCCACACCTT CCGCCAGCTT GACCTGCTGC TCCCCAAGCT GGCACGTGGC 
TCCCTGTCAG CGGGGGATAA GGACCGGGTG GGCAAGGTCA AAGGTCGCCT GTGGAAGCTG 
TTGTCCCCAA CCAGGCTGGC CAGCCGGGCC CACCGGAGCA GATGGCTGGA GAGTTACCTG 
CTGCACCTGG AGGAGATGGG CGTGTCGGAG GAGATGCAGG CTCGCGCCCT GGTGCTGCAG 
CTCTGGGCCA CACAGGGGAA CATGGGTCCT GCTGCCTTCT GGCTCCTGCT CTTTCTCCTC 
AAGAACCCCG AGGCCCTGGC CGCTGTCCGT GGAGAGCTAG AGACTGTCCT TTTGGGAGCA 
GAGCAGCCCA TATCACAGAT GACCACCCTC CCACAGAAAG TTTTGGACAG CATGCCTGTG 
CTGGACAGCG TGCTGAGTGA GAGCCTCAGG CTCACCGCCG CGCCCTTCAT CACCCGCGAG 
GTCGTGGCCG ACCTGGCCTT GCCCATGGCG GATGGGCGGG AATTCAGCCT GCGACGTGGG 
GACCGCCTCC TCCTCTTTCC CTTCCTGAGT CCCCAGAAGG ACCCGGAGAT CTACACAGAC 
CCGGAGGTAT TTAAATACAA CCGATTCCTG AACCCTGATG GATCAGAGAA GAAAGACTTT 
TACAAAGATG GGAAGCGACT GAAGAACTAC AGCTTGCCGT GGGGAGCAGG GCATAACCAG 
TGCCTGGGGA AGGGTTACGC CGTCAACAGC ATCAAACAAT TCGTGTTCCT TGTGCTGACG 
CAGTTTGACC TGGAGCTGAT CACCCCGGAC GTGGACATCC CCGAGTTTGA CCTCAGCAGG 
TATGGCTTTG GGCTGATGCA GCCGGAACAC GATGTGCCTG TCCGTTACCG CATCCGGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGIS ( XM_015474114.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329600.1 Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa221447
Clone ID Related Accession (Same CDS sequence)	XM_015474114.2
Accession Version	XM_015474114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prostacyclin synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT TCAGACCCAG GCGACCTGGC GAGCCTCCCC TGGACCTGGG CAGTATCCCC 
TGGCTGGGTC ACGCCTTGGA GTTTGGAAAA GATGCTGCCG GCTTCCTCAC TAGGATGAAG 
GAGAAGCATG GGGACATCTT TACTGTGCTG GTGGGCGGCA GGCACGTCAC GGTCCTCCTG 
GATCCCCACT CCTACGACGC GGTGGTGTGG GAGCCCCGCA GCAGGCTGGA CTTCCACGCC 
TACGCCGTCT TCCTCATGGA GAGGATTTTC GACGTGCAGC TTCCGCATTA TAACCCCGGT 
GATGAAAAGT CCAAGATGAA ACCGACTCTC CTCCACAAAG AGCTGCAGGC GCTCACGGAT 
GCCATGTATA CCAACCTCCG CACTGTCCTG CTGGGTGACA CCGTGGAAGC GGGCAGTGGC 
TGGCACGAAA TGGGGCTCCT CGAGTTCTCC TACGGCTTCC TGCTCAGAGC CGGCTACCTG 
ACCCAGTACG GCGTTGAGGC ACCGCCACAC ACCCAGGAGA GCCAGGCCCA GGACCGTGTC 
CACTCAGCCG ACGTCTTCCA CACCTTCCGC CAGCTTGACC TGCTGCTCCC CAAGCTGGCA 
CGTGGCTCCC TGTCAGCGGG GGATAAGGAC CGGGTGGGCA AGGTCAAAGG TCGCCTGTGG 
AAGCTGTTGT CCCCAACCAG GCTGGCCAGC CGGGCCCACC GGAGCAGATG GCTGGAGAGT 
TACCTGCTGC ACCTGGAGGA GATGGGCGTG TCGGAGGAGA TGCAGGCTCG CGCCCTGGTG 
CTGCAGCTCT GGGCCACACA GGGGAACATG GGTCCTGCTG CCTTCTGGCT CCTGCTCTTT 
CTCCTCAAGA ACCCCGAGGC CCTGGCCGCT GTCCGTGGAG AGCTAGAGAC TGTCCTTTTG 
GGAGCAGAGC AGCCCATATC ACAGATGACC ACCCTCCCAC AGAAAGTTTT GGACAGCATG 
CCTGTGCTGG ACAGCGTGCT GAGTGAGAGC CTCAGGCTCA CCGCCGCGCC CTTCATCACC 
CGCGAGGTCG TGGCCGACCT GGCCTTGCCC ATGGCGGATG GGCGGGAATT CAGCCTGCGA 
CGTGGGGACC GCCTCCTCCT CTTTCCCTTC CTGAGTCCCC AGAAGGACCC GGAGATCTAC 
ACAGACCCGG AGGTATTTAA ATACAACCGA TTCCTGAACC CTGATGGATC AGAGAAGAAA 
GACTTTTACA AAGATGGGAA GCGACTGAAG AACTACAGCT TGCCGTGGGG AGCAGGGCAT 
AACCAGTGCC TGGGGAAGGG TTACGCCGTC AACAGCATCA AACAATTCGT GTTCCTTGTG 
CTGACGCAGT TTGACCTGGA GCTGATCACC CCGGACGTGG ACATCCCCGA GTTTGACCTC 
AGCAGGTATG GCTTTGGGCT GATGCAGCCG GAACACGATG TGCCTGTCCG TTACCGCATC 
CGGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329600.1
CDS	23..1471
Translation	MAGFRPRRPGEPPLDLGSIPWLGHALEFGKDAAGFLTRMKEKHGDIFTVLVGGRHVTVLLDPHSYDAVVWEPRSRLDFHAYAVFLMERIFDVQLPHYNPGDEKSKMKPTLLHKELQALTDAMYTNLRTVLLGDTVEAGSGWHEMGLLEFSYGFLLRAGYLTQYGVEAPPHTQESQAQDRVHSADVFHTFRQLDLLLPKLARGSLSAGDKDRVGKVKGRLWKLLSPTRLASRAHRSRWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELETVLLGAEQPISQMTTLPQKVLDSMPVLDSVLSESLRLTAAPFITREVVADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYSLPWGAGHNQCLGKGYAVNSIKQFVFLVLTQFDLELITPDVDIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Target ORF information:

RefSeq Version	XM_015474114.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474114.2

ORF Insert Sequence:

ATGGCTGGAT TCAGACCCAG GCGACCTGGC GAGCCTCCCC TGGACCTGGG CAGTATCCCC 
TGGCTGGGTC ACGCCTTGGA GTTTGGAAAA GATGCTGCCG GCTTCCTCAC TAGGATGAAG 
GAGAAGCATG GGGACATCTT TACTGTGCTG GTGGGCGGCA GGCACGTCAC GGTCCTCCTG 
GATCCCCACT CCTACGACGC GGTGGTGTGG GAGCCCCGCA GCAGGCTGGA CTTCCACGCC 
TACGCCGTCT TCCTCATGGA GAGGATTTTC GACGTGCAGC TTCCGCATTA TAACCCCGGT 
GATGAAAAGT CCAAGATGAA ACCGACTCTC CTCCACAAAG AGCTGCAGGC GCTCACGGAT 
GCCATGTATA CCAACCTCCG CACTGTCCTG CTGGGTGACA CCGTGGAAGC GGGCAGTGGC 
TGGCACGAAA TGGGGCTCCT CGAGTTCTCC TACGGCTTCC TGCTCAGAGC CGGCTACCTG 
ACCCAGTACG GCGTTGAGGC ACCGCCACAC ACCCAGGAGA GCCAGGCCCA GGACCGTGTC 
CACTCAGCCG ACGTCTTCCA CACCTTCCGC CAGCTTGACC TGCTGCTCCC CAAGCTGGCA 
CGTGGCTCCC TGTCAGCGGG GGATAAGGAC CGGGTGGGCA AGGTCAAAGG TCGCCTGTGG 
AAGCTGTTGT CCCCAACCAG GCTGGCCAGC CGGGCCCACC GGAGCAGATG GCTGGAGAGT 
TACCTGCTGC ACCTGGAGGA GATGGGCGTG TCGGAGGAGA TGCAGGCTCG CGCCCTGGTG 
CTGCAGCTCT GGGCCACACA GGGGAACATG GGTCCTGCTG CCTTCTGGCT CCTGCTCTTT 
CTCCTCAAGA ACCCCGAGGC CCTGGCCGCT GTCCGTGGAG AGCTAGAGAC TGTCCTTTTG 
GGAGCAGAGC AGCCCATATC ACAGATGACC ACCCTCCCAC AGAAAGTTTT GGACAGCATG 
CCTGTGCTGG ACAGCGTGCT GAGTGAGAGC CTCAGGCTCA CCGCCGCGCC CTTCATCACC 
CGCGAGGTCG TGGCCGACCT GGCCTTGCCC ATGGCGGATG GGCGGGAATT CAGCCTGCGA 
CGTGGGGACC GCCTCCTCCT CTTTCCCTTC CTGAGTCCCC AGAAGGACCC GGAGATCTAC 
ACAGACCCGG AGGTATTTAA ATACAACCGA TTCCTGAACC CTGATGGATC AGAGAAGAAA 
GACTTTTACA AAGATGGGAA GCGACTGAAG AACTACAGCT TGCCGTGGGG AGCAGGGCAT 
AACCAGTGCC TGGGGAAGGG TTACGCCGTC AACAGCATCA AACAATTCGT GTTCCTTGTG 
CTGACGCAGT TTGACCTGGA GCTGATCACC CCGGACGTGG ACATCCCCGA GTTTGACCTC 
AGCAGGTATG GCTTTGGGCT GATGCAGCCG GAACACGATG TGCCTGTCCG TTACCGCATC 
CGGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGIS ( XM_015474114.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329600.1 Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92296
Clone ID Related Accession (Same CDS sequence)	XM_015474114.1
Accession Version	XM_015474114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostacyclin synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT TCAGACCCAG GCGACCTGGC GAGCCTCCCC TGGACCTGGG CAGTATCCCC 
TGGCTGGGTC ACGCCTTGGA GTTTGGAAAA GATGCTGCCG GCTTCCTCAC TAGGATGAAG 
GAGAAGCATG GGGACATCTT TACTGTGCTG GTGGGCGGCA GGCACGTCAC GGTCCTCCTG 
GATCCCCACT CCTACGACGC GGTGGTGTGG GAGCCCCGCA GCAGGCTGGA CTTCCACGCC 
TACGCCGTCT TCCTCATGGA GAGGATTTTC GACGTGCAGC TTCCGCATTA TAACCCCGGT 
GATGAAAAGT CCAAGATGAA ACCGACTCTC CTCCACAAAG AGCTGCAGGC GCTCACGGAT 
GCCATGTATA CCAACCTCCG CACTGTCCTG CTGGGTGACA CCGTGGAAGC GGGCAGTGGC 
TGGCACGAAA TGGGGCTCCT CGAGTTCTCC TACGGCTTCC TGCTCAGAGC CGGCTACCTG 
ACCCAGTACG GCGTTGAGGC ACCGCCACAC ACCCAGGAGA GCCAGGCCCA GGACCGTGTC 
CACTCAGCCG ACGTCTTCCA CACCTTCCGC CAGCTTGACC TGCTGCTCCC CAAGCTGGCA 
CGTGGCTCCC TGTCAGCGGG GGATAAGGAC CGGGTGGGCA AGGTCAAAGG TCGCCTGTGG 
AAGCTGTTGT CCCCAACCAG GCTGGCCAGC CGGGCCCACC GGAGCAGATG GCTGGAGAGT 
TACCTGCTGC ACCTGGAGGA GATGGGCGTG TCGGAGGAGA TGCAGGCTCG CGCCCTGGTG 
CTGCAGCTCT GGGCCACACA GGGGAACATG GGTCCTGCCG CCTTCTGGCT CCTGCTCTTT 
CTCCTCAAGA ACCCCGAGGC CCTGGCCGCT GTCCGTGGAG AGCTAGAGAC TGTCCTTTTG 
GGAGCAGAGC AGCCCATATC ACAGATGACC ACCCTCCCAC AGAAAGTTTT GGACAGCATG 
CCTGTGCTGG ACAGCGTGCT GAGTGAGAGC CTCAGGCTCA CCGCCGCGCC CTTCATCACC 
CGCGAGGTCG TGGCCGACCT GGCCTTGCCC ATGGCGGATG GGCGGGAATT CAGCCTGCGA 
CGTGGGGACC GCCTCCTCCT CTTTCCCTTC CTGAGTCCCC AGAAGGACCC GGAGATCTAC 
ACAGACCCGG AGGTATTTAA ATACAACCGA TTCCTGAACC CTGATGGATC AGAGAAGAAA 
GACTTTTACA AAGATGGGAA GCGACTGAAG AACTACAGCT TGCCGTGGGG AGCAGGGCAT 
AACCAGTGCC TGGGGAAGGG TTACGCCGTC AACAGCATCA AACAATTCGT GTTCCTTGTG 
CTGACGCAGT TTGACCTGGA GCTGATCACC CCGGACGTGG ACATCCCCGA GTTTGACCTC 
AGCAGGTATG GCTTTGGGCT GATGCAGCCG GAACACGATG TGCCTGTCCG TTACCGCATC 
CGGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329600.1
CDS	23..1471
Translation	MAGFRPRRPGEPPLDLGSIPWLGHALEFGKDAAGFLTRMKEKHGDIFTVLVGGRHVTVLLDPHSYDAVVWEPRSRLDFHAYAVFLMERIFDVQLPHYNPGDEKSKMKPTLLHKELQALTDAMYTNLRTVLLGDTVEAGSGWHEMGLLEFSYGFLLRAGYLTQYGVEAPPHTQESQAQDRVHSADVFHTFRQLDLLLPKLARGSLSAGDKDRVGKVKGRLWKLLSPTRLASRAHRSRWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELETVLLGAEQPISQMTTLPQKVLDSMPVLDSVLSESLRLTAAPFITREVVADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYSLPWGAGHNQCLGKGYAVNSIKQFVFLVLTQFDLELITPDVDIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Target ORF information:

RefSeq Version	XM_015474114.1
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474114.1

ORF Insert Sequence:

ATGGCTGGAT TCAGACCCAG GCGACCTGGC GAGCCTCCCC TGGACCTGGG CAGTATCCCC 
TGGCTGGGTC ACGCCTTGGA GTTTGGAAAA GATGCTGCCG GCTTCCTCAC TAGGATGAAG 
GAGAAGCATG GGGACATCTT TACTGTGCTG GTGGGCGGCA GGCACGTCAC GGTCCTCCTG 
GATCCCCACT CCTACGACGC GGTGGTGTGG GAGCCCCGCA GCAGGCTGGA CTTCCACGCC 
TACGCCGTCT TCCTCATGGA GAGGATTTTC GACGTGCAGC TTCCGCATTA TAACCCCGGT 
GATGAAAAGT CCAAGATGAA ACCGACTCTC CTCCACAAAG AGCTGCAGGC GCTCACGGAT 
GCCATGTATA CCAACCTCCG CACTGTCCTG CTGGGTGACA CCGTGGAAGC GGGCAGTGGC 
TGGCACGAAA TGGGGCTCCT CGAGTTCTCC TACGGCTTCC TGCTCAGAGC CGGCTACCTG 
ACCCAGTACG GCGTTGAGGC ACCGCCACAC ACCCAGGAGA GCCAGGCCCA GGACCGTGTC 
CACTCAGCCG ACGTCTTCCA CACCTTCCGC CAGCTTGACC TGCTGCTCCC CAAGCTGGCA 
CGTGGCTCCC TGTCAGCGGG GGATAAGGAC CGGGTGGGCA AGGTCAAAGG TCGCCTGTGG 
AAGCTGTTGT CCCCAACCAG GCTGGCCAGC CGGGCCCACC GGAGCAGATG GCTGGAGAGT 
TACCTGCTGC ACCTGGAGGA GATGGGCGTG TCGGAGGAGA TGCAGGCTCG CGCCCTGGTG 
CTGCAGCTCT GGGCCACACA GGGGAACATG GGTCCTGCCG CCTTCTGGCT CCTGCTCTTT 
CTCCTCAAGA ACCCCGAGGC CCTGGCCGCT GTCCGTGGAG AGCTAGAGAC TGTCCTTTTG 
GGAGCAGAGC AGCCCATATC ACAGATGACC ACCCTCCCAC AGAAAGTTTT GGACAGCATG 
CCTGTGCTGG ACAGCGTGCT GAGTGAGAGC CTCAGGCTCA CCGCCGCGCC CTTCATCACC 
CGCGAGGTCG TGGCCGACCT GGCCTTGCCC ATGGCGGATG GGCGGGAATT CAGCCTGCGA 
CGTGGGGACC GCCTCCTCCT CTTTCCCTTC CTGAGTCCCC AGAAGGACCC GGAGATCTAC 
ACAGACCCGG AGGTATTTAA ATACAACCGA TTCCTGAACC CTGATGGATC AGAGAAGAAA 
GACTTTTACA AAGATGGGAA GCGACTGAAG AACTACAGCT TGCCGTGGGG AGCAGGGCAT 
AACCAGTGCC TGGGGAAGGG TTACGCCGTC AACAGCATCA AACAATTCGT GTTCCTTGTG 
CTGACGCAGT TTGACCTGGA GCTGATCACC CCGGACGTGG ACATCCCCGA GTTTGACCTC 
AGCAGGTATG GCTTTGGGCT GATGCAGCCG GAACACGATG TGCCTGTCCG TTACCGCATC 
CGGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGIS ( XM_015474115.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329601.1 Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X2, mRNA.

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CloneID	OBa92297
Clone ID Related Accession (Same CDS sequence)	XM_015474115.1
Accession Version	XM_015474115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostacyclin synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGCATGGGGA CATCTTTACT GTGCTGGTGG GCGGCAGGCA CGTCACGGTC 
CTCCTGGATC CCCACTCCTA CGACGCGGTG GTGTGGGAGC CCCGCAGCAG GCTGGACTTC 
CACGCCTACG CCGTCTTCCT CATGGAGAGG ATTTTCGACG TGCAGCTTCC GCATTATAAC 
CCCGGTGATG AAAAGTCCAA GATGAAACCG ACTCTCCTCC ACAAAGAGCT GCAGGCGCTC 
ACGGATGCCA TGTATACCAA CCTCCGCACT GTCCTGCTGG GTGACACCGT GGAAGCGGGC 
AGTGGCTGGC ACGAAATGGG GCTCCTCGAG TTCTCCTACG GCTTCCTGCT CAGAGCCGGC 
TACCTGACCC AGTACGGCGT TGAGGCACCG CCACACACCC AGGAGAGCCA GGCCCAGGAC 
CGTGTCCACT CAGCCGACGT CTTCCACACC TTCCGCCAGC TTGACCTGCT GCTCCCCAAG 
CTGGCACGTG GCTCCCTGTC AGCGGGGGAT AAGGACCGGG TGGGCAAGGT CAAAGGTCGC 
CTGTGGAAGC TGTTGTCCCC AACCAGGCTG GCCAGCCGGG CCCACCGGAG CAGATGGCTG 
GAGAGTTACC TGCTGCACCT GGAGGAGATG GGCGTGTCGG AGGAGATGCA GGCTCGCGCC 
CTGGTGCTGC AGCTCTGGGC CACACAGGGG AACATGGGTC CTGCCGCCTT CTGGCTCCTG 
CTCTTTCTCC TCAAGAACCC CGAGGCCCTG GCCGCTGTCC GTGGAGAGCT AGAGACTGTC 
CTTTTGGGAG CAGAGCAGCC CATATCACAG ATGACCACCC TCCCACAGAA AGTTTTGGAC 
AGCATGCCTG TGCTGGACAG CGTGCTGAGT GAGAGCCTCA GGCTCACCGC CGCGCCCTTC 
ATCACCCGCG AGGTCGTGGC CGACCTGGCC TTGCCCATGG CGGATGGGCG GGAATTCAGC 
CTGCGACGTG GGGACCGCCT CCTCCTCTTT CCCTTCCTGA GTCCCCAGAA GGACCCGGAG 
ATCTACACAG ACCCGGAGGT ATTTAAATAC AACCGATTCC TGAACCCTGA TGGATCAGAG 
AAGAAAGACT TTTACAAAGA TGGGAAGCGA CTGAAGAACT ACAGCTTGCC GTGGGGAGCA 
GGGCATAACC AGTGCCTGGG GAAGGGTTAC GCCGTCAACA GCATCAAACA ATTCGTGTTC 
CTTGTGCTGA CGCAGTTTGA CCTGGAGCTG ATCACCCCGG ACGTGGACAT CCCCGAGTTT 
GACCTCAGCA GGTATGGCTT TGGGCTGATG CAGCCGGAAC ACGATGTGCC TGTCCGTTAC 
CGCATCCGGC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329601.1
CDS	274..1608
Translation	MKEKHGDIFTVLVGGRHVTVLLDPHSYDAVVWEPRSRLDFHAYAVFLMERIFDVQLPHYNPGDEKSKMKPTLLHKELQALTDAMYTNLRTVLLGDTVEAGSGWHEMGLLEFSYGFLLRAGYLTQYGVEAPPHTQESQAQDRVHSADVFHTFRQLDLLLPKLARGSLSAGDKDRVGKVKGRLWKLLSPTRLASRAHRSRWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELETVLLGAEQPISQMTTLPQKVLDSMPVLDSVLSESLRLTAAPFITREVVADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYSLPWGAGHNQCLGKGYAVNSIKQFVFLVLTQFDLELITPDVDIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Target ORF information:

RefSeq Version	XM_015474115.1
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin I2 (prostacyclin) synthase (PTGIS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474115.1

ORF Insert Sequence:

ATGAAGGAGA AGCATGGGGA CATCTTTACT GTGCTGGTGG GCGGCAGGCA CGTCACGGTC 
CTCCTGGATC CCCACTCCTA CGACGCGGTG GTGTGGGAGC CCCGCAGCAG GCTGGACTTC 
CACGCCTACG CCGTCTTCCT CATGGAGAGG ATTTTCGACG TGCAGCTTCC GCATTATAAC 
CCCGGTGATG AAAAGTCCAA GATGAAACCG ACTCTCCTCC ACAAAGAGCT GCAGGCGCTC 
ACGGATGCCA TGTATACCAA CCTCCGCACT GTCCTGCTGG GTGACACCGT GGAAGCGGGC 
AGTGGCTGGC ACGAAATGGG GCTCCTCGAG TTCTCCTACG GCTTCCTGCT CAGAGCCGGC 
TACCTGACCC AGTACGGCGT TGAGGCACCG CCACACACCC AGGAGAGCCA GGCCCAGGAC 
CGTGTCCACT CAGCCGACGT CTTCCACACC TTCCGCCAGC TTGACCTGCT GCTCCCCAAG 
CTGGCACGTG GCTCCCTGTC AGCGGGGGAT AAGGACCGGG TGGGCAAGGT CAAAGGTCGC 
CTGTGGAAGC TGTTGTCCCC AACCAGGCTG GCCAGCCGGG CCCACCGGAG CAGATGGCTG 
GAGAGTTACC TGCTGCACCT GGAGGAGATG GGCGTGTCGG AGGAGATGCA GGCTCGCGCC 
CTGGTGCTGC AGCTCTGGGC CACACAGGGG AACATGGGTC CTGCCGCCTT CTGGCTCCTG 
CTCTTTCTCC TCAAGAACCC CGAGGCCCTG GCCGCTGTCC GTGGAGAGCT AGAGACTGTC 
CTTTTGGGAG CAGAGCAGCC CATATCACAG ATGACCACCC TCCCACAGAA AGTTTTGGAC 
AGCATGCCTG TGCTGGACAG CGTGCTGAGT GAGAGCCTCA GGCTCACCGC CGCGCCCTTC 
ATCACCCGCG AGGTCGTGGC CGACCTGGCC TTGCCCATGG CGGATGGGCG GGAATTCAGC 
CTGCGACGTG GGGACCGCCT CCTCCTCTTT CCCTTCCTGA GTCCCCAGAA GGACCCGGAG 
ATCTACACAG ACCCGGAGGT ATTTAAATAC AACCGATTCC TGAACCCTGA TGGATCAGAG 
AAGAAAGACT TTTACAAAGA TGGGAAGCGA CTGAAGAACT ACAGCTTGCC GTGGGGAGCA 
GGGCATAACC AGTGCCTGGG GAAGGGTTAC GCCGTCAACA GCATCAAACA ATTCGTGTTC 
CTTGTGCTGA CGCAGTTTGA CCTGGAGCTG ATCACCCCGG ACGTGGACAT CCCCGAGTTT 
GACCTCAGCA GGTATGGCTT TGGGCTGATG CAGCCGGAAC ACGATGTGCC TGTCCGTTAC 
CGCATCCGGC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGIS ( XM_015474115.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329601.2 Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X1, mRNA.

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CloneID	OBa221446
Clone ID Related Accession (Same CDS sequence)	XM_015474115.2
Accession Version	XM_015474115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prostacyclin synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAG GAAATGTTAA GTACTGTCAA GAAAAACAAA CCGGTTCAAG GCAACAGACA 
AGCAACCTGC CAAGGCGACC TGGCGAGCCT CCCCTGGACC TGGGCAGTAT CCCCTGGCTG 
GGTCACGCCT TGGAGTTTGG AAAAGATGCT GCCGGCTTCC TCACTAGGAT GAAGGAGAAG 
CATGGGGACA TCTTTACTGT GCTGGTGGGC GGCAGGCACG TCACGGTCCT CCTGGATCCC 
CACTCCTACG ACGCGGTGGT GTGGGAGCCC CGCAGCAGGC TGGACTTCCA CGCCTACGCC 
GTCTTCCTCA TGGAGAGGAT TTTCGACGTG CAGCTTCCGC ATTATAACCC CGGTGATGAA 
AAGTCCAAGA TGAAACCGAC TCTCCTCCAC AAAGAGCTGC AGGCGCTCAC GGATGCCATG 
TATACCAACC TCCGCACTGT CCTGCTGGGT GACACCGTGG AAGCGGGCAG TGGCTGGCAC 
GAAATGGGGC TCCTCGAGTT CTCCTACGGC TTCCTGCTCA GAGCCGGCTA CCTGACCCAG 
TACGGCGTTG AGGCACCGCC ACACACCCAG GAGAGCCAGG CCCAGGACCG TGTCCACTCA 
GCCGACGTCT TCCACACCTT CCGCCAGCTT GACCTGCTGC TCCCCAAGCT GGCACGTGGC 
TCCCTGTCAG CGGGGGATAA GGACCGGGTG GGCAAGGTCA AAGGTCGCCT GTGGAAGCTG 
TTGTCCCCAA CCAGGCTGGC CAGCCGGGCC CACCGGAGCA GATGGCTGGA GAGTTACCTG 
CTGCACCTGG AGGAGATGGG CGTGTCGGAG GAGATGCAGG CTCGCGCCCT GGTGCTGCAG 
CTCTGGGCCA CACAGGGGAA CATGGGTCCT GCTGCCTTCT GGCTCCTGCT CTTTCTCCTC 
AAGAACCCCG AGGCCCTGGC CGCTGTCCGT GGAGAGCTAG AGACTGTCCT TTTGGGAGCA 
GAGCAGCCCA TATCACAGAT GACCACCCTC CCACAGAAAG TTTTGGACAG CATGCCTGTG 
CTGGACAGCG TGCTGAGTGA GAGCCTCAGG CTCACCGCCG CGCCCTTCAT CACCCGCGAG 
GTCGTGGCCG ACCTGGCCTT GCCCATGGCG GATGGGCGGG AATTCAGCCT GCGACGTGGG 
GACCGCCTCC TCCTCTTTCC CTTCCTGAGT CCCCAGAAGG ACCCGGAGAT CTACACAGAC 
CCGGAGGTAT TTAAATACAA CCGATTCCTG AACCCTGATG GATCAGAGAA GAAAGACTTT 
TACAAAGATG GGAAGCGACT GAAGAACTAC AGCTTGCCGT GGGGAGCAGG GCATAACCAG 
TGCCTGGGGA AGGGTTACGC CGTCAACAGC ATCAAACAAT TCGTGTTCCT TGTGCTGACG 
CAGTTTGACC TGGAGCTGAT CACCCCGGAC GTGGACATCC CCGAGTTTGA CCTCAGCAGG 
TATGGCTTTG GGCTGATGCA GCCGGAACAC GATGTGCCTG TCCGTTACCG CATCCGGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329601.2
CDS	16..1518
Translation	MIAGNVKYCQEKQTGSRQQTSNLPRRPGEPPLDLGSIPWLGHALEFGKDAAGFLTRMKEKHGDIFTVLVGGRHVTVLLDPHSYDAVVWEPRSRLDFHAYAVFLMERIFDVQLPHYNPGDEKSKMKPTLLHKELQALTDAMYTNLRTVLLGDTVEAGSGWHEMGLLEFSYGFLLRAGYLTQYGVEAPPHTQESQAQDRVHSADVFHTFRQLDLLLPKLARGSLSAGDKDRVGKVKGRLWKLLSPTRLASRAHRSRWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELETVLLGAEQPISQMTTLPQKVLDSMPVLDSVLSESLRLTAAPFITREVVADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYSLPWGAGHNQCLGKGYAVNSIKQFVFLVLTQFDLELITPDVDIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Target ORF information:

RefSeq Version	XM_015474115.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin I2 synthase (PTGIS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474115.2

ORF Insert Sequence:

ATGATAGCAG GAAATGTTAA GTACTGTCAA GAAAAACAAA CCGGTTCAAG GCAACAGACA 
AGCAACCTGC CAAGGCGACC TGGCGAGCCT CCCCTGGACC TGGGCAGTAT CCCCTGGCTG 
GGTCACGCCT TGGAGTTTGG AAAAGATGCT GCCGGCTTCC TCACTAGGAT GAAGGAGAAG 
CATGGGGACA TCTTTACTGT GCTGGTGGGC GGCAGGCACG TCACGGTCCT CCTGGATCCC 
CACTCCTACG ACGCGGTGGT GTGGGAGCCC CGCAGCAGGC TGGACTTCCA CGCCTACGCC 
GTCTTCCTCA TGGAGAGGAT TTTCGACGTG CAGCTTCCGC ATTATAACCC CGGTGATGAA 
AAGTCCAAGA TGAAACCGAC TCTCCTCCAC AAAGAGCTGC AGGCGCTCAC GGATGCCATG 
TATACCAACC TCCGCACTGT CCTGCTGGGT GACACCGTGG AAGCGGGCAG TGGCTGGCAC 
GAAATGGGGC TCCTCGAGTT CTCCTACGGC TTCCTGCTCA GAGCCGGCTA CCTGACCCAG 
TACGGCGTTG AGGCACCGCC ACACACCCAG GAGAGCCAGG CCCAGGACCG TGTCCACTCA 
GCCGACGTCT TCCACACCTT CCGCCAGCTT GACCTGCTGC TCCCCAAGCT GGCACGTGGC 
TCCCTGTCAG CGGGGGATAA GGACCGGGTG GGCAAGGTCA AAGGTCGCCT GTGGAAGCTG 
TTGTCCCCAA CCAGGCTGGC CAGCCGGGCC CACCGGAGCA GATGGCTGGA GAGTTACCTG 
CTGCACCTGG AGGAGATGGG CGTGTCGGAG GAGATGCAGG CTCGCGCCCT GGTGCTGCAG 
CTCTGGGCCA CACAGGGGAA CATGGGTCCT GCTGCCTTCT GGCTCCTGCT CTTTCTCCTC 
AAGAACCCCG AGGCCCTGGC CGCTGTCCGT GGAGAGCTAG AGACTGTCCT TTTGGGAGCA 
GAGCAGCCCA TATCACAGAT GACCACCCTC CCACAGAAAG TTTTGGACAG CATGCCTGTG 
CTGGACAGCG TGCTGAGTGA GAGCCTCAGG CTCACCGCCG CGCCCTTCAT CACCCGCGAG 
GTCGTGGCCG ACCTGGCCTT GCCCATGGCG GATGGGCGGG AATTCAGCCT GCGACGTGGG 
GACCGCCTCC TCCTCTTTCC CTTCCTGAGT CCCCAGAAGG ACCCGGAGAT CTACACAGAC 
CCGGAGGTAT TTAAATACAA CCGATTCCTG AACCCTGATG GATCAGAGAA GAAAGACTTT 
TACAAAGATG GGAAGCGACT GAAGAACTAC AGCTTGCCGT GGGGAGCAGG GCATAACCAG 
TGCCTGGGGA AGGGTTACGC CGTCAACAGC ATCAAACAAT TCGTGTTCCT TGTGCTGACG 
CAGTTTGACC TGGAGCTGAT CACCCCGGAC GTGGACATCC CCGAGTTTGA CCTCAGCAGG 
TATGGCTTTG GGCTGATGCA GCCGGAACAC GATGTGCCTG TCCGTTACCG CATCCGGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Specific nitration at tyrosine 430 revealed by high resolution mass spectrometry as basis for redox regulation of bovine prostacyclin synthase.
The Journal of biological chemistry278(15)12813-9(2003 Apr)
Schmidt P,Youhnovski N,Daiber A,Balan A,Arsic M,Bachschmid M,Przybylski M,Ullrich V

Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.
The Journal of biological chemistry269(31)19897-903(1994 Aug)
Hara S,Miyata A,Yokoyama C,Inoue H,Brugger R,Lottspeich F,Ullrich V,Tanabe T

Molecular cloning and characterization of bovine prostacyclin synthase.
Biochemical and biophysical research communications203(1)59-66(1994 Aug)
Pereira B,Wu KK,Wang LH

Bovine prostacyclin synthase: purification and isolation of partial cDNA.
Biochemical and biophysical research communications197(3)1041-8(1993 Dec)
Pereira B,Wu KK,Wang LH

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PTGIS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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