Ptgis cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptgis
Entrez Gene ID	25527
Full Name	prostaglandin I2 synthase
Synonyms	PGIS
General protein information	Preferred Names prostaglandin I2 synthase Names prostacyclin synthase prostaglandin I2 (prostacyclin) synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	catalyzes the conversion of prostaglandin H2 to prostacyclin in prostaglandin biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031557.2	NP_113745.1	prostacyclin synthase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PTGIS	XP_005635412.1
Mus musculus (house mouse)	Ptgis	NP_032994.1
Pan troglodytes (chimpanzee)	PTGIS	XP_514713.3
Rattus norvegicus (Norway rat)	Ptgis	NP_113745.1
Xenopus tropicalis (tropical clawed frog)	ptgis	XP_002933228.2
Homo sapiens (human)	PTGIS	NP_000952.1
	PTGIS	XP_001098575.1
Bos taurus (cattle)	PTGIS	NP_776869.1
Danio rerio (zebrafish)	ptgis	NP_001104630.1

Gene Ontology
Bibliography

Related articles in PubMed

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

Selective activation of lectin-like oxidized low-density lipoprotein receptor-1 mediates C-reactive protein-evoked endothelial vasodilator dysfunction in coronary arterioles.
Hein TW, Qamirani E, Ren Y, Xu X, Thengchaisri N, Kuo L
Circulation research114(1)92-100(2014 Jan)

Molecular mechanisms regulating the vascular prostacyclin pathways and their adaptation during pregnancy and in the newborn.
Majed BH, Khalil RA
Pharmacological reviews64(3)540-82(2012 Jul)

Hypoxia upregulates PGI-synthase and increases PGI? release in human vascular cells exposed to inflammatory stimuli.
Camacho M, Rodr?guez C, Guadall A, Alcolea S, Orriols M, Escudero JR, Mart?nez-Gonz?lez J, Vila L
Journal of lipid research52(4)720-31(2011 Apr)

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: mechanisms of COX-2 inhibitor risk to heart disease.
Ruan CH, So SP, Ruan KH
Life sciences88(1-2)24-30(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene.
Title: Variable number tandem repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.

PGIS overexpression apparently protects insulin-producing cells against cytokine toxicity via suppression of endoplasmic reticulum and mitochondrial stress-mediated cell death pathways
Title: Protection against cytokine toxicity through endoplasmic reticulum and mitochondrial stress prevention by prostacyclin synthase overexpression in insulin-producing cells.

Prostaglandin D synthase and prostacyclin synthase are present and could be regulated by steroids in the rat uterus during pregnancy. Endometrium could be significant source of PGD(2) and PGI(2) at specific times during pregnancy.
Title: Regulation of prostaglandin D synthase and prostacyclin synthase in the endometrium of cyclic, pregnant, and pseudopregnant rats and their regulation by sex steroids.

Overall, this study demonstrated that engagement of TLR4 with LPS induces production of PGI2 via Akt and generates VEGF through IP receptor.
Title: Activation of toll-like receptor 4 modulates vascular endothelial growth factor synthesis through prostacyclin-IP signaling.

Prostacyclin synthase protects the brain by enhancing PGI2 synthesis and creating a favorable PGI2/TXA2 ratio
Title: Induction of prostacyclin/PGI2 synthase expression after cerebral ischemia-reperfusion.

The following Ptgis gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptgis cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08097	NM_031557.2 Latest version!	Rattus norvegicus prostaglandin I2 synthase (Ptgis), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$293.30 $419.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptgis ( NM_031557.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113745.1 Rattus norvegicus prostaglandin I2 synthase (Ptgis), mRNA.

Price & Availability↑

CloneID	ORa08097
Clone ID Related Accession (Same CDS sequence)	NM_031557.2
Accession Version	NM_031557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	prostacyclin synthase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061814.1. On Feb 9, 2004 this sequence version replaced NM_031557.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061814.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906353, SAMN01906356 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTGGG CCGCTCTGCT CGGCCTTCTG GCCGTGTTAT TACTGTTGCT GCTGCTGCTG 
AGCCGCCGGC GCGCGCGGCG ACCCGGTGAG CCTCCGTTGG ACCTGGGCAG CATCCCCTGG 
CTGGGCCATG CCTTGGAGTT TGGGAAGGAT GCCGCCAGCT TCCTTACCAG GATGAAGGAG 
AAGCACGGTG ACATATTTAC TGTGCTGGTG GGGGGCAGGT ATGTCACTGT CCTCCTGGAC 
CCACACTCTT ACGACACAGT GGTGTGGGAT CTGCGTACAC GGCTGGACTT CCACCCCTAC 
GCCATCTTCC TCATGGAGAG GATTTTTGAT CTGCAGCTTC CAAATTTCAA CCCCAGCGAG 
GAGAAGGCCA GGATGAAGCC GACGCTCATG CACAAAGACC TGCAGGCGCT CACGGAAGCC 
ATGTATACCA ACCTGCGCAC CGTCCTGCTG GGTGACTCCA CAGAAGGAGG CAGTGGCTGG 
CAGGAGAAAG GTCTGCTTGA GTTCTCCTAC AGCTCCCTGC TCAGCGCTGG CTACCTGACC 
CTGTATGGAG TGGAGGCCTC ACCACGCACC CACGAGAGTC AGGCCCTCGA CCGTGACCAC 
TCAGCCGACG TTTTCCGCAC CTTCCGCCAG CTGGATCTGA TGCTCCCCAA ACTGGCTCGA 
GGCTCCTTGT CAGTGGGGGA TAAAGACCAT GCATGCAGTG TCAAAAGCCG CCTGTGGAAG 
CTGCTGTCTC CAGCCGGACT GGCCTCCAGG GCTGACCGGA GCAGCTGGTT GGAGAGTTAC 
CTGAGACATC TTGAGGAGAT GGGGGTATCA GAGGACATGC AGGCCCGGGC ACTGGTGCTG 
CAGCTCTGGG CCACACAAGG GAATATGGGA CCCACGGCTT TCTGGCTCCT CCTCTTCCTC 
CTCAAGAACC CGGAAGCCCT CGATGCTGTC CATGCAGAGC TGAAACGCAT CGTCTGGCAA 
GCAGAGAAGC CTGTTTTACA GATGACCGCA CTCCCGCAGA AGATTCTAGA CAGCATGCCT 
GTGCTAGACA GTGTGCTCAA TGAGACCCTC CGGCTCACGG CCGCCCCCTT CATCACCCGT 
GAGGTCATGG CAGACCTGGC CTTGCCTATG GCAGACAGGA GGGAATTCTC TCTTCGACGC 
GGTGACCGCC TTCTCCTCTT TCCTTTCCTG AGTCCCCAGA AGGACCCAGA AATCTACACA 
GAACCTGAAG TCTTTAAATA CAACCGGTTC TTGAACCCAG ATGGGTCAGA AAAGAAAGAT 
TTTTACAAAG ATGGGAAACG GCTAAAGAAT TACAACATGC CTTGGGGCGC AGGGCACAAC 
CAGTGCCTGG GGAAGAGCTA TGCCATCAAC AGCATCAAAC AGTTTGTGGT CCTGCTGCTG 
ACTCATTTCG ACCTGGAGCT GGTCAGTGAG GACACAGAGG TCCCGGAGTT TGACCTTAGC 
AGATATGGCT TTGGCCTGAT GCAGCCGGAG GAAGATGTGC CCATCCGGTA CCGTACCAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113745.1
CDS	76..1581
Translation	MSWAALLGLLAVLLLLLLLLSRRRARRPGEPPLDLGSIPWLGHALEFGKDAASFLTRMKEKHGDIFTVLVGGRYVTVLLDPHSYDTVVWDLRTRLDFHPYAIFLMERIFDLQLPNFNPSEEKARMKPTLMHKDLQALTEAMYTNLRTVLLGDSTEGGSGWQEKGLLEFSYSSLLSAGYLTLYGVEASPRTHESQALDRDHSADVFRTFRQLDLMLPKLARGSLSVGDKDHACSVKSRLWKLLSPAGLASRADRSSWLESYLRHLEEMGVSEDMQARALVLQLWATQGNMGPTAFWLLLFLLKNPEALDAVHAELKRIVWQAEKPVLQMTALPQKILDSMPVLDSVLNETLRLTAAPFITREVMADLALPMADRREFSLRRGDRLLLFPFLSPQKDPEIYTEPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNQCLGKSYAINSIKQFVVLLLTHFDLELVSEDTEVPEFDLSRYGFGLMQPEEDVPIRYRTRL

Target ORF information:

RefSeq Version	NM_031557.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prostaglandin I2 synthase (Ptgis), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031557.2

ORF Insert Sequence:

ATGTCCTGGG CCGCTCTGCT CGGCCTTCTG GCCGTGTTAT TACTGTTGCT GCTGCTGCTG 
AGCCGCCGGC GCGCGCGGCG ACCCGGTGAG CCTCCGTTGG ACCTGGGCAG CATCCCCTGG 
CTGGGCCATG CCTTGGAGTT TGGGAAGGAT GCCGCCAGCT TCCTTACCAG GATGAAGGAG 
AAGCACGGTG ACATATTTAC TGTGCTGGTG GGGGGCAGGT ATGTCACTGT CCTCCTGGAC 
CCACACTCTT ACGACACAGT GGTGTGGGAT CTGCGTACAC GGCTGGACTT CCACCCCTAC 
GCCATCTTCC TCATGGAGAG GATTTTTGAT CTGCAGCTTC CAAATTTCAA CCCCAGCGAG 
GAGAAGGCCA GGATGAAGCC GACGCTCATG CACAAAGACC TGCAGGCGCT CACGGAAGCC 
ATGTATACCA ACCTGCGCAC CGTCCTGCTG GGTGACTCCA CAGAAGGAGG CAGTGGCTGG 
CAGGAGAAAG GTCTGCTTGA GTTCTCCTAC AGCTCCCTGC TCAGCGCTGG CTACCTGACC 
CTGTATGGAG TGGAGGCCTC ACCACGCACC CACGAGAGTC AGGCCCTCGA CCGTGACCAC 
TCAGCCGACG TTTTCCGCAC CTTCCGCCAG CTGGATCTGA TGCTCCCCAA ACTGGCTCGA 
GGCTCCTTGT CAGTGGGGGA TAAAGACCAT GCATGCAGTG TCAAAAGCCG CCTGTGGAAG 
CTGCTGTCTC CAGCCGGACT GGCCTCCAGG GCTGACCGGA GCAGCTGGTT GGAGAGTTAC 
CTGAGACATC TTGAGGAGAT GGGGGTATCA GAGGACATGC AGGCCCGGGC ACTGGTGCTG 
CAGCTCTGGG CCACACAAGG GAATATGGGA CCCACGGCTT TCTGGCTCCT CCTCTTCCTC 
CTCAAGAACC CGGAAGCCCT CGATGCTGTC CATGCAGAGC TGAAACGCAT CGTCTGGCAA 
GCAGAGAAGC CTGTTTTACA GATGACCGCA CTCCCGCAGA AGATTCTAGA CAGCATGCCT 
GTGCTAGACA GTGTGCTCAA TGAGACCCTC CGGCTCACGG CCGCCCCCTT CATCACCCGT 
GAGGTCATGG CAGACCTGGC CTTGCCTATG GCAGACAGGA GGGAATTCTC TCTTCGACGC 
GGTGACCGCC TTCTCCTCTT TCCTTTCCTG AGTCCCCAGA AGGACCCAGA AATCTACACA 
GAACCTGAAG TCTTTAAATA CAACCGGTTC TTGAACCCAG ATGGGTCAGA AAAGAAAGAT 
TTTTACAAAG ATGGGAAACG GCTAAAGAAT TACAACATGC CTTGGGGCGC AGGGCACAAC 
CAGTGCCTGG GGAAGAGCTA TGCCATCAAC AGCATCAAAC AGTTTGTGGT CCTGCTGCTG 
ACTCATTTCG ACCTGGAGCT GGTCAGTGAG GACACAGAGG TCCCGGAGTT TGACCTTAGC 
AGATATGGCT TTGGCCTGAT GCAGCCGGAG GAAGATGTGC CCATCCGGTA CCGTACCAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

Selective activation of lectin-like oxidized low-density lipoprotein receptor-1 mediates C-reactive protein-evoked endothelial vasodilator dysfunction in coronary arterioles.
Circulation research114(1)92-100(2014 Jan)
Hein TW,Qamirani E,Ren Y,Xu X,Thengchaisri N,Kuo L

Molecular mechanisms regulating the vascular prostacyclin pathways and their adaptation during pregnancy and in the newborn.
Pharmacological reviews64(3)540-82(2012 Jul)
Majed BH,Khalil RA

Hypoxia upregulates PGI-synthase and increases PGI? release in human vascular cells exposed to inflammatory stimuli.
Journal of lipid research52(4)720-31(2011 Apr)
Camacho M,Rodr?guez C,Guadall A,Alcolea S,Orriols M,Escudero JR,Mart?nez-Gonz?lez J,Vila L

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: mechanisms of COX-2 inhibitor risk to heart disease.
Life sciences88(1-2)24-30(2011 Jan)
Ruan CH,So SP,Ruan KH

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Ptgis cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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