PDE2A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE2A
Entrez Gene ID	281971
Full Name	phosphodiesterase 2A
Synonyms	CGS-PDE,cGSPDE
General protein information	Preferred Names phosphodiesterase 2A Names cGMP-dependent 3',5'-cyclic phosphodiesterase c-GMP stimulated phosphodiesterase cyclic GMP-stimulated phosphodiesterase phosphodiesterase 2A, cGMP-stimulated
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001143846.1	NP_001137318.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
NM_001256273.1	NP_001243202.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1
XM_024975017.1	XP_024830785.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X1
XM_024975020.1	XP_024830788.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X4
XM_024975018.1	XP_024830786.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X2
XM_024975019.1	XP_024830787.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE2A	NP_001139681.1
	PDE2A	NP_001124298.1
Xenopus tropicalis (tropical clawed frog)	pde2a	NP_001072607.1
Pan troglodytes (chimpanzee)	PDE2A	XP_001174746.1
Bos taurus (cattle)	PDE2A	NP_001137318.1
Mus musculus (house mouse)	Pde2a	NP_001137320.1
Rattus norvegicus (Norway rat)	Pde2a	NP_001137319.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008304	XP_317162.4
Canis lupus familiaris (dog)	PDE2A	XP_851951.2

Gene Ontology
Bibliography

Related articles in PubMed

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Molecular determinants for cyclic nucleotide binding to the regulatory domains of phosphodiesterase 2A.
Wu AY, Tang XB, Martinez SE, Ikeda K, Beavo JA
The Journal of biological chemistry279(36)37928-38(2004 Sep)

Molecular cloning of a cyclic GMP-stimulated cyclic nucleotide phosphodiesterase cDNA. Identification and distribution of isozyme variants.
Sonnenburg WK, Mullaney PJ, Beavo JA
The Journal of biological chemistry266(26)17655-61(1991 Sep)

Amino acid sequence of the cyclic GMP stimulated cyclic nucleotide phosphodiesterase from bovine heart.
Trong HL, Beier N, Sonnenburg WK, Stroop SD, Walsh KA, Beavo JA, Charbonneau H
Biochemistry29(44)10280-8(1990 Nov)

Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species.
Charbonneau H, Beier N, Walsh KA, Beavo JA
Proceedings of the National Academy of Sciences of the United States of America83(24)9308-12(1986 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDE2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44107	NM_001143846.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa44517	NM_001256273.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa222656	XM_024975020.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222653	XM_024975017.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222654	XM_024975018.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222655	XM_024975019.1 Latest version!	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE2A ( NM_001143846.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137318.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa44107
Clone ID Related Accession (Same CDS sequence)	NM_001143846.1
Accession Version	NM_001143846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L49503.1 and EE898164.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform, PDE2A3 (see PMID:9210593). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAGCCGCC GCCGCCGCCG 
CAGCCATGCG CCGACAGCCT GCAGGATGCT TTGCTGAGCC TGGGCTCCGT CATTGACGTT 
GCAGGCTTGC AACAGGCTGT CAAGGAGGCC CTGTCGGCTG TGCTTCCCAA AGTGGAGACG 
GTCTACACCT ACCTGCTGGA TGGGGAATCC CGGCTGGTGT GTGAGGAGCC CCCCCACGAG 
CTGCCCCAGG AGGGGAAAGT GCGAGAGGCT GTGATCTCCC GGAAGCGGCT GGGCTGCAAT 
GGACTGGGCC CCTCAGACCT GCCTGGGAAG CCCTTGGCAA GGCTGGTGGC TCCACTGGCT 
CCTGACACCC AAGTGCTGGT CATACCGCTG GTGGACAAGG AGGCCGGGGC TGTGGCAGCT 
GTCATCTTGG TGCACTGTGG TCAGCTGAGT GACAATGAGG AGTGGAGCCT GCAAGCTGTG 
GAGAAGCATA CCCTGGTGGC CCTGAAAAGG GTGCAGGCCT TGCAGCAGCG CGAGTCCAGC 
GTGGCCCCGG AAGCGACCCA GAATCCTCCG GAGGAGGCAG CGGGAGACCA GAAGGGTGGG 
GTCGCATACA CAGACCAAGA CCGAAAGATC CTGCAGCTTT GCGGGGAGCT CTACGACCTG 
GATGCATCTT CCCTGCAGCT CAAAGTCCTC CAATATCTGC AACAGGAGAC CCAGGCATCC 
CGCTGCTGCC TGCTGCTGGT ATCCGAGGAC AATCTTCAGC TCTCCTGCAA GGTCATTGGA 
GATAAAGTAC TGGAGGAAGA GATCAGCTTT CCGTTGACCA CAGGACGCCT GGGCCAAGTG 
GTGGAAGACA AGAAGTCTAT CCAGCTGAAA GATCTCACCT CCGAGGATAT GCAACAGCTG 
CAAAGCATGT TGGGCTGTGA GGTGCAGGCC ATGCTCTGTG TCCCTGTCAT CAGCCGGGCC 
ACTGACCAGG TCGTGGCCCT GGCCTGTGCC TTCAACAAGC TCGGAGGAGA CTTGTTCACA 
GACCAGGACG AGCACGTGAT CCAGCACTGC TTCCACTACA CCAGCACAGT GCTCACCAGC 
ACCCTGGCCT TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG 
GCGAAGAACC TCTTCACTCA TCTGGATGAC GTCTCCGTGC TGCTCCAGGA GATCATCACA 
GAGGCCAGGA ACCTCAGCAA TGCTGAGATC TGCTCTGTGT TCCTGCTGGA TCAGAACGAG 
CTGGTGGCCA AGGTGTTCGA TGGGGGTGTG GTGGAAGATG AGAGCTATGA GATCCGCATT 
CCCGCTGACC AGGGCATCGC GGGTCATGTG GCGACCACCG GCCAGATCCT AAACATCCCA 
GATGCTTACG CACATCCGCT TTTCTACCGA GGCGTGGACG ACAGCACCGG CTTCCGGACG 
CGCAACATCC TCTGCTTCCC CATCAAGAAC GAGAACCAGG AGGTCATCGG TGTGGCCGAG 
CTGGTGAACA AGATCAATGG ACCATGGTTC AGCAAGTTTG ATGAAGACCT GGCTACAGCC 
TTCTCCATCT ACTGTGGCAT CAGCATTGCC CATTCCCTCC TATACAAGAA AGTGAATGAG 
GCGCAGTATC GCAGCCACCT TGCCAATGAG ATGATGATGT ACCACATGAA GGTCTCTGAT 
GACGAGTACA CCAAACTTCT CCATGACGGG ATCCAGCCTG TGGCTGCCAT CGACTCCAAC 
TTTGCCAGTT TCACATACAC TCCTCGCTCT CTGCCCGAGG ATGACACTTC CATGGCCATC 
CTGAGCATGC TGCAGGACAT GAATTTCATC AATAACTACA AAATTGACTG CCCGACACTG 
GCCCGGTTCT GTTTGATGGT GAAGAAGGGC TACCGGGATC CCCCCTACCA CAACTGGATG 
CACGCCTTTT CTGTCTCCCA CTTCTGCTAC CTGCTCTACA AGAACCTGGA GCTCACCAAC 
TACCTCGAGG ACATGGAGAT CTTTGCCTTG TTTATTTCCT GCATGTGTCA CGACCTGGAC 
CACAGAGGCA CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC CGCGCTCTAC 
AGCTCGGAAG GCTCTGTCAT GGAGAGGCAC CACTTCGCTC AGGCCATTGC CATCCTCAAC 
ACCCACGGCT GCAACATCTT TGACCACTTC TCCCGGAAGG ATTATCAGCG CATGTTGGAC 
CTGATGCGGG ACATCATCTT GGCCACAGAT CTGGCCCACC ACCTCCGCAT CTTCAAGGAC 
CTCCAAAAGA TGGCCGAAGT GGGCTATGAT CGAACCAACA AGCAGCACCA CAGCCTCCTT 
CTCTGCCTCC TTATGACCTC CTGTGACCTC TCTGACCAGA CCAAGGGCTG GAAGACCACG 
AGGAAGATCG CGGAGCTGAT CTACAAAGAG TTCTTCTCCC AGGGAGACTT GGAGAAGGCC 
ATGGGCAACA GGCCGATGGA GATGATGGAC CGTGAGAAGG CCTACATCCC CGAGCTGCAG 
ATCAGCTTCA TGGAGCACAT CGCAATGCCC ATCTACAAGC TGCTGCAAGA CCTGTTCCCC 
AAGGCGGCCG AGTTGTACGA ACGCGTGGCC TCTAATCGTG AGCACTGGAC CAAGGTGTCA 
CACAAGTTCA CCATCCGAGG CCTCCCGAGC AACAACTCGT TGGACTTCCT GGACGAGGAG 
TATGAGGTGC CTGACCTGGA TGGCGCTAGG GCTCCCATCA ATGGCTGTTG CAGCCTTGAT 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137318.1
CDS	181..3009
Translation	MGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTDQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	NM_001143846.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143846.1

ORF Insert Sequence:

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAGCCGCC GCCGCCGCCG 
CAGCCATGCG CCGACAGCCT GCAGGATGCT TTGCTGAGCC TGGGCTCCGT CATTGACGTT 
GCAGGCTTGC AACAGGCTGT CAAGGAGGCC CTGTCGGCTG TGCTTCCCAA AGTGGAGACG 
GTCTACACCT ACCTGCTGGA TGGGGAATCC CGGCTGGTGT GTGAGGAGCC CCCCCACGAG 
CTGCCCCAGG AGGGGAAAGT GCGAGAGGCT GTGATCTCCC GGAAGCGGCT GGGCTGCAAT 
GGACTGGGCC CCTCAGACCT GCCTGGGAAG CCCTTGGCAA GGCTGGTGGC TCCACTGGCT 
CCTGACACCC AAGTGCTGGT CATACCGCTG GTGGACAAGG AGGCCGGGGC TGTGGCAGCT 
GTCATCTTGG TGCACTGTGG TCAGCTGAGT GACAATGAGG AGTGGAGCCT GCAAGCTGTG 
GAGAAGCATA CCCTGGTGGC CCTGAAAAGG GTGCAGGCCT TGCAGCAGCG CGAGTCCAGC 
GTGGCCCCGG AAGCGACCCA GAATCCTCCG GAGGAGGCAG CGGGAGACCA GAAGGGTGGG 
GTCGCATACA CAGACCAAGA CCGAAAGATC CTGCAGCTTT GCGGGGAGCT CTACGACCTG 
GATGCATCTT CCCTGCAGCT CAAAGTCCTC CAATATCTGC AACAGGAGAC CCAGGCATCC 
CGCTGCTGCC TGCTGCTGGT ATCCGAGGAC AATCTTCAGC TCTCCTGCAA GGTCATTGGA 
GATAAAGTAC TGGAGGAAGA GATCAGCTTT CCGTTGACCA CAGGACGCCT GGGCCAAGTG 
GTGGAAGACA AGAAGTCTAT CCAGCTGAAA GATCTCACCT CCGAGGATAT GCAACAGCTG 
CAAAGCATGT TGGGCTGTGA GGTGCAGGCC ATGCTCTGTG TCCCTGTCAT CAGCCGGGCC 
ACTGACCAGG TCGTGGCCCT GGCCTGTGCC TTCAACAAGC TCGGAGGAGA CTTGTTCACA 
GACCAGGACG AGCACGTGAT CCAGCACTGC TTCCACTACA CCAGCACAGT GCTCACCAGC 
ACCCTGGCCT TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG 
GCGAAGAACC TCTTCACTCA TCTGGATGAC GTCTCCGTGC TGCTCCAGGA GATCATCACA 
GAGGCCAGGA ACCTCAGCAA TGCTGAGATC TGCTCTGTGT TCCTGCTGGA TCAGAACGAG 
CTGGTGGCCA AGGTGTTCGA TGGGGGTGTG GTGGAAGATG AGAGCTATGA GATCCGCATT 
CCCGCTGACC AGGGCATCGC GGGTCATGTG GCGACCACCG GCCAGATCCT AAACATCCCA 
GATGCTTACG CACATCCGCT TTTCTACCGA GGCGTGGACG ACAGCACCGG CTTCCGGACG 
CGCAACATCC TCTGCTTCCC CATCAAGAAC GAGAACCAGG AGGTCATCGG TGTGGCCGAG 
CTGGTGAACA AGATCAATGG ACCATGGTTC AGCAAGTTTG ATGAAGACCT GGCTACAGCC 
TTCTCCATCT ACTGTGGCAT CAGCATTGCC CATTCCCTCC TATACAAGAA AGTGAATGAG 
GCGCAGTATC GCAGCCACCT TGCCAATGAG ATGATGATGT ACCACATGAA GGTCTCTGAT 
GACGAGTACA CCAAACTTCT CCATGACGGG ATCCAGCCTG TGGCTGCCAT CGACTCCAAC 
TTTGCCAGTT TCACATACAC TCCTCGCTCT CTGCCCGAGG ATGACACTTC CATGGCCATC 
CTGAGCATGC TGCAGGACAT GAATTTCATC AATAACTACA AAATTGACTG CCCGACACTG 
GCCCGGTTCT GTTTGATGGT GAAGAAGGGC TACCGGGATC CCCCCTACCA CAACTGGATG 
CACGCCTTTT CTGTCTCCCA CTTCTGCTAC CTGCTCTACA AGAACCTGGA GCTCACCAAC 
TACCTCGAGG ACATGGAGAT CTTTGCCTTG TTTATTTCCT GCATGTGTCA CGACCTGGAC 
CACAGAGGCA CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC CGCGCTCTAC 
AGCTCGGAAG GCTCTGTCAT GGAGAGGCAC CACTTCGCTC AGGCCATTGC CATCCTCAAC 
ACCCACGGCT GCAACATCTT TGACCACTTC TCCCGGAAGG ATTATCAGCG CATGTTGGAC 
CTGATGCGGG ACATCATCTT GGCCACAGAT CTGGCCCACC ACCTCCGCAT CTTCAAGGAC 
CTCCAAAAGA TGGCCGAAGT GGGCTATGAT CGAACCAACA AGCAGCACCA CAGCCTCCTT 
CTCTGCCTCC TTATGACCTC CTGTGACCTC TCTGACCAGA CCAAGGGCTG GAAGACCACG 
AGGAAGATCG CGGAGCTGAT CTACAAAGAG TTCTTCTCCC AGGGAGACTT GGAGAAGGCC 
ATGGGCAACA GGCCGATGGA GATGATGGAC CGTGAGAAGG CCTACATCCC CGAGCTGCAG 
ATCAGCTTCA TGGAGCACAT CGCAATGCCC ATCTACAAGC TGCTGCAAGA CCTGTTCCCC 
AAGGCGGCCG AGTTGTACGA ACGCGTGGCC TCTAATCGTG AGCACTGGAC CAAGGTGTCA 
CACAAGTTCA CCATCCGAGG CCTCCCGAGC AACAACTCGT TGGACTTCCT GGACGAGGAG 
TATGAGGTGC CTGACCTGGA TGGCGCTAGG GCTCCCATCA ATGGCTGTTG CAGCCTTGAT 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE2A ( NM_001256273.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001243202.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa44517
Clone ID Related Accession (Same CDS sequence)	NM_001256273.1
Accession Version	NM_001256273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M73512.1 and CO881442.1. On Jan 14, 2012 this sequence version replaced NR_026573.1. Transcript Variant: This variant (2) uses alternate 5' exon structure and thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform, PDE2A1 (see PMID: 9210593) has a distinct and shorter N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: M73512.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCCGAC AGCCTGCAGC CAGCCGGGAC CTCTTTGCAC AGGAGCCAGT GCCCCCAGGG 
AGTGGAGACG GCGCATTGCA GGATGCTTTG CTGAGCCTGG GCTCCGTCAT CGACGTTGCA 
GGCTTGCAAC AGGCTGTCAA GGAGGCCCTG TCGGCTGTGC TTCCCAAAGT GGAGACGGTC 
TACACCTACC TGCTGGATGG GGAATCCCGG CTGGTGTGTG AGGAGCCCCC CCACGAGCTG 
CCCCAGGAGG GGAAAGTGCG AGAGGCTGTG ATCTCCCGGA AGCGGCTGGG CTGCAATGGA 
CTGGGCCCCT CAGACCTGCC TGGGAAGCCC TTGGCAAGGC TGGTGGCTCC ACTGGCTCCT 
GACACCCAAG TGCTGGTCAT ACCGCTGGTG GACAAGGAGG CCGGGGCTGT GGCAGCTGTC 
ATCTTGGTGC ACTGTGGTCA GCTGAGTGAC AATGAGGAGT GGAGCCTGCA AGCTGTGGAG 
AAGCATACCC TGGTGGCCCT GAAAAGGGTG CAGGCCTTGC AGCAGCGCGA GTCCAGCGTG 
GCCCCGGAAG CGACCCAGAA TCCTCCGGAG GAGGCAGCGG GAGACCAGAA GGGTGGGGTC 
GCATACACAA ACCAAGACCG AAAGATCCTG CAGCTTTGCG GGGAGCTCTA CGACCTGGAT 
GCATCTTCCC TGCAGCTCAA AGTCCTCCAA TATCTGCAAC AGGAGACCCA GGCATCCCGC 
TGCTGCCTGC TGCTGGTATC CGAGGACAAT CTTCAGCTCT CCTGCAAGGT CATTGGAGAT 
AAAGTACTGG AGGAAGAGAT CAGCTTTCCG TTGACCACAG GACGCCTGGG CCAAGTGGTG 
GAAGACAAGA AGTCTATCCA GCTGAAAGAT CTCACCTCCG AGGATATGCA ACAGCTGCAA 
AGCATGTTGG GCTGTGAGGT GCAGGCCATG CTCTGTGTCC CTGTCATCAG CCGGGCCACT 
GACCAGGTCG TGGCCCTGGC CTGTGCCTTC AACAAGCTCG GAGGAGACTT GTTCACAGAC 
CAGGACGAGC ACGTGATCCA GCACTGCTTC CACTACACCA GCACAGTGCT CACCAGCACC 
CTGGCCTTCC AGAAGGAGCA GAAGCTCAAG TGTGAGTGCC AGGCTCTTCT CCAAGTGGCG 
AAGAACCTCT TCACTCATCT GGATGACGTC TCCGTGCTGC TCCAGGAGAT CATCACAGAG 
GCCAGGAACC TCAGCAATGC TGAGATCTGC TCTGTGTTCC TGCTGGATCA GAACGAGCTG 
GTGGCCAAGG TGTTCGATGG GGGTGTGGTG GAAGATGAGA GCTATGAGAT CCGCATTCCC 
GCTGACCAGG GCATCGCGGG TCATGTGGCG ACCACCGGCC AGATCCTAAA CATCCCAGAT 
GCTTACGCAC ATCCGCTTTT CTACCGAGGC GTGGACGACA GCACCGGCTT CCGGACGCGC 
AACATCCTCT GCTTCCCCAT CAAGAACGAG AACCAGGAGG TCATCGGTGT GGCCGAGCTG 
GTGAACAAGA TCAATGGACC ATGGTTCAGC AAGTTTGATG AAGACCTGGC TACAGCCTTC 
TCCATCTACT GTGGCATCAG CATTGCCCAT TCCCTCCTAT ACAAGAAAGT GAATGAGGCG 
CAGTATCGCA GCCACCTTGC CAATGAGATG ATGATGTACC ACATGAAGGT CTCTGATGAC 
GAGTACACCA AACTTCTCCA TGACGGGATC CAGCCTGTGG CTGCCATCGA CTCCAACTTT 
GCCAGTTTCA CATACACTCC TCGCTCTCTG CCCGAGGATG ACACTTCCAT GGCCATCCTG 
AGCATGCTGC AGGACATGAA TTTCATCAAT AACTACAAAA TTGACTGCCC GACACTGGCC 
CGGTTCTGTT TGATGGTGAA GAAGGGCTAC CGGGATCCCC CCTACCACAA CTGGATGCAC 
GCCTTTTCTG TCTCCCACTT CTGCTACCTG CTCTACAAGA ACCTGGAGCT CACCAACTAC 
CTCGAGGACA TGGAGATCTT TGCCTTGTTT ATTTCCTGCA TGTGTCACGA CCTGGACCAC 
AGAGGCACAA ACAACTCCTT CCAGGTGGCC TCGAAATCTG TGCTGGCCGC GCTCTACAGC 
TCGGAAGGCT CTGTCATGGA GAGGCACCAC TTCGCTCAGG CCATTGCCAT CCTCAACACC 
CACGGCTGCA ACATCTTTGA CCACTTCTCC CGGAAGGATT ATCAGCGCAT GTTGGACCTG 
ATGCGGGACA TCATCTTGGC CACAGATCTG GCCCACCACC TCCGCATCTT CAAGGACCTC 
CAAAAGATGG CCGAAGTGGG CTATGATCGA ACCAACAAGC AGCACCACAG CCTCCTTCTC 
TGCCTCCTTA TGACCTCCTG TGACCTCTCT GACCAGACCA AGGGCTGGAA GACCACGAGG 
AAGATCGCGG AGCTGATCTA CAAAGAGTTC TTCTCCCAGG GAGACTTGGA GAAGGCCATG 
GGCAACAGGC CGATGGAGAT GATGGACCGT GAGAAGGCCT ACATCCCCGA GCTGCAGATC 
AGCTTCATGG AGCACATCGC AATGCCCATC TACAAGCTGC TGCAAGACCT GTTCCCCAAG 
GCGGCCGAGT TGTACGAACG CGTGGCCTCT AATCGTGAGC ACTGGACCAA GGTGTCACAC 
AAGTTCACCA TCCGAGGCCT CCCGAGCAAC AACTCGTTGG ACTTCCTGGA CGAGGAGTAT 
GAGGTGCCTG ACCTGGATGG CGCTAGGGCT CCCATCAATG GCTGTTGCAG CCTTGATGCT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243202.1
CDS	150..2915
Translation	MRRQPAASRDLFAQEPVPPGSGDGALQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTNQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	NM_001256273.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256273.1

ORF Insert Sequence:

ATGCGCCGAC AGCCTGCAGC CAGCCGGGAC CTCTTTGCAC AGGAGCCAGT GCCCCCAGGG 
AGTGGAGACG GCGCATTGCA GGATGCTTTG CTGAGCCTGG GCTCCGTCAT CGACGTTGCA 
GGCTTGCAAC AGGCTGTCAA GGAGGCCCTG TCGGCTGTGC TTCCCAAAGT GGAGACGGTC 
TACACCTACC TGCTGGATGG GGAATCCCGG CTGGTGTGTG AGGAGCCCCC CCACGAGCTG 
CCCCAGGAGG GGAAAGTGCG AGAGGCTGTG ATCTCCCGGA AGCGGCTGGG CTGCAATGGA 
CTGGGCCCCT CAGACCTGCC TGGGAAGCCC TTGGCAAGGC TGGTGGCTCC ACTGGCTCCT 
GACACCCAAG TGCTGGTCAT ACCGCTGGTG GACAAGGAGG CCGGGGCTGT GGCAGCTGTC 
ATCTTGGTGC ACTGTGGTCA GCTGAGTGAC AATGAGGAGT GGAGCCTGCA AGCTGTGGAG 
AAGCATACCC TGGTGGCCCT GAAAAGGGTG CAGGCCTTGC AGCAGCGCGA GTCCAGCGTG 
GCCCCGGAAG CGACCCAGAA TCCTCCGGAG GAGGCAGCGG GAGACCAGAA GGGTGGGGTC 
GCATACACAA ACCAAGACCG AAAGATCCTG CAGCTTTGCG GGGAGCTCTA CGACCTGGAT 
GCATCTTCCC TGCAGCTCAA AGTCCTCCAA TATCTGCAAC AGGAGACCCA GGCATCCCGC 
TGCTGCCTGC TGCTGGTATC CGAGGACAAT CTTCAGCTCT CCTGCAAGGT CATTGGAGAT 
AAAGTACTGG AGGAAGAGAT CAGCTTTCCG TTGACCACAG GACGCCTGGG CCAAGTGGTG 
GAAGACAAGA AGTCTATCCA GCTGAAAGAT CTCACCTCCG AGGATATGCA ACAGCTGCAA 
AGCATGTTGG GCTGTGAGGT GCAGGCCATG CTCTGTGTCC CTGTCATCAG CCGGGCCACT 
GACCAGGTCG TGGCCCTGGC CTGTGCCTTC AACAAGCTCG GAGGAGACTT GTTCACAGAC 
CAGGACGAGC ACGTGATCCA GCACTGCTTC CACTACACCA GCACAGTGCT CACCAGCACC 
CTGGCCTTCC AGAAGGAGCA GAAGCTCAAG TGTGAGTGCC AGGCTCTTCT CCAAGTGGCG 
AAGAACCTCT TCACTCATCT GGATGACGTC TCCGTGCTGC TCCAGGAGAT CATCACAGAG 
GCCAGGAACC TCAGCAATGC TGAGATCTGC TCTGTGTTCC TGCTGGATCA GAACGAGCTG 
GTGGCCAAGG TGTTCGATGG GGGTGTGGTG GAAGATGAGA GCTATGAGAT CCGCATTCCC 
GCTGACCAGG GCATCGCGGG TCATGTGGCG ACCACCGGCC AGATCCTAAA CATCCCAGAT 
GCTTACGCAC ATCCGCTTTT CTACCGAGGC GTGGACGACA GCACCGGCTT CCGGACGCGC 
AACATCCTCT GCTTCCCCAT CAAGAACGAG AACCAGGAGG TCATCGGTGT GGCCGAGCTG 
GTGAACAAGA TCAATGGACC ATGGTTCAGC AAGTTTGATG AAGACCTGGC TACAGCCTTC 
TCCATCTACT GTGGCATCAG CATTGCCCAT TCCCTCCTAT ACAAGAAAGT GAATGAGGCG 
CAGTATCGCA GCCACCTTGC CAATGAGATG ATGATGTACC ACATGAAGGT CTCTGATGAC 
GAGTACACCA AACTTCTCCA TGACGGGATC CAGCCTGTGG CTGCCATCGA CTCCAACTTT 
GCCAGTTTCA CATACACTCC TCGCTCTCTG CCCGAGGATG ACACTTCCAT GGCCATCCTG 
AGCATGCTGC AGGACATGAA TTTCATCAAT AACTACAAAA TTGACTGCCC GACACTGGCC 
CGGTTCTGTT TGATGGTGAA GAAGGGCTAC CGGGATCCCC CCTACCACAA CTGGATGCAC 
GCCTTTTCTG TCTCCCACTT CTGCTACCTG CTCTACAAGA ACCTGGAGCT CACCAACTAC 
CTCGAGGACA TGGAGATCTT TGCCTTGTTT ATTTCCTGCA TGTGTCACGA CCTGGACCAC 
AGAGGCACAA ACAACTCCTT CCAGGTGGCC TCGAAATCTG TGCTGGCCGC GCTCTACAGC 
TCGGAAGGCT CTGTCATGGA GAGGCACCAC TTCGCTCAGG CCATTGCCAT CCTCAACACC 
CACGGCTGCA ACATCTTTGA CCACTTCTCC CGGAAGGATT ATCAGCGCAT GTTGGACCTG 
ATGCGGGACA TCATCTTGGC CACAGATCTG GCCCACCACC TCCGCATCTT CAAGGACCTC 
CAAAAGATGG CCGAAGTGGG CTATGATCGA ACCAACAAGC AGCACCACAG CCTCCTTCTC 
TGCCTCCTTA TGACCTCCTG TGACCTCTCT GACCAGACCA AGGGCTGGAA GACCACGAGG 
AAGATCGCGG AGCTGATCTA CAAAGAGTTC TTCTCCCAGG GAGACTTGGA GAAGGCCATG 
GGCAACAGGC CGATGGAGAT GATGGACCGT GAGAAGGCCT ACATCCCCGA GCTGCAGATC 
AGCTTCATGG AGCACATCGC AATGCCCATC TACAAGCTGC TGCAAGACCT GTTCCCCAAG 
GCGGCCGAGT TGTACGAACG CGTGGCCTCT AATCGTGAGC ACTGGACCAA GGTGTCACAC 
AAGTTCACCA TCCGAGGCCT CCCGAGCAAC AACTCGTTGG ACTTCCTGGA CGAGGAGTAT 
GAGGTGCCTG ACCTGGATGG CGCTAGGGCT CCCATCAATG GCTGTTGCAG CCTTGATGCT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE2A ( XM_024975020.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830788.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa222656
Clone ID Related Accession (Same CDS sequence)	XM_024975020.1
Accession Version	XM_024975020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGAC AGCCTGCAGC CAGCCGGGAC CTCTTTGCAC AGGAGCCAGT GCCCCCAGGG 
AGTGGAGACG GCGCATTGCA GGATGCTTTG CTGAGCCTGG GCTCCGTCAT CGACGTTGCA 
GGCTTGCAAC AGGCTGTCAA GGAGGCCCTG TCGGCTGTGC TTCCCAAAGT GGAGACGGTC 
TACACCTACC TGCTGGATGG GGAATCCCGG CTGGTGTGTG AGGAGCCCCC CCACGAGCTG 
CCCCAGGAGG GGAAAGTGCG AGAGGCTGTG ATCTCCCGGA AGCGGCTGGG CTGCAATGGA 
CTGGGCCCCT CAGACCTGCC TGGGAAGCCC TTGGCAAGGC TGGTGGCTCC ACTGGCTCCT 
GACACCCAAG TGCTGGTCAT ACCGCTGGTG GACAAGGAGG CCGGGGCTGT GGCAGCTGTC 
ATCTTGGTGC ACTGTGGTCA GCTGAGTGAC AATGAGGAGT GGAGCCTGCA AGCTGTGGAG 
AAGCATACCC TGGTGGCCCT GAAAAGGGTG CAGGCCTTGC AGCAGCGCGA GTCCAGCGTG 
GCCCCGGAAG CGACCCAGAA TCCTCCGGAG GAGGCAGCGG GAGACCAGAA GGGTGGGGTC 
GCATACACAG ACCAAGACCG AAAGATCCTG CAGCTTTGCG GGGAGCTCTA CGACCTGGAT 
GCATCTTCCC TGCAGCTCAA AGTCCTCCAA TATCTGCAAC AGGAGACCCA GGCATCCCGC 
TGCTGCCTGC TGCTGGTATC CGAGGACAAT CTTCAGCTCT CCTGCAAGGT CATTGGAGAT 
AAAGTACTGG AGGAAGAGAT CAGCTTTCCG TTGACCACAG GACGCCTGGG CCAAGTGGTG 
GAAGACAAGA AGTCTATCCA GCTGAAAGAT CTCACCTCCG AGGATATGCA ACAGCTGCAA 
AGCATGTTGG GCTGTGAGGT GCAGGCCATG CTCTGTGTCC CTGTCATCAG CCGGGCCACT 
GACCAGGTCG TGGCCCTGGC CTGTGCCTTC AACAAGCTCG GAGGAGACTT GTTCACAGAC 
CAGGACGAGC ACGTGATCCA GCACTGCTTC CACTACACCA GCACAGTGCT CACCAGCACC 
CTGGCCTTCC AGAAGGAGCA GAAGCTCAAG TGTGAGTGCC AGGCTCTTCT CCAAGTGGCG 
AAGAACCTCT TCACTCATCT GGATGACGTC TCCGTGCTGC TCCAGGAGAT CATCACAGAG 
GCCAGGAACC TCAGCAATGC TGAGATCTGC TCTGTGTTCC TGCTGGATCA GAACGAGCTG 
GTGGCCAAGG TGTTCGATGG GGGTGTGGTG GAAGATGAGA GCTATGAGAT CCGCATTCCC 
GCTGACCAGG GCATCGCGGG TCATGTGGCG ACCACCGGCC AGATCCTAAA CATCCCAGAT 
GCTTACGCAC ATCCGCTTTT CTACCGAGGC GTGGACGACA GCACCGGCTT CCGGACGCGC 
AACATCCTCT GCTTCCCCAT CAAGAACGAG AACCAGGAGG TCATCGGTGT GGCCGAGCTG 
GTGAACAAGA TCAATGGACC ATGGTTCAGC AAGTTTGATG AAGACCTGGC TACAGCCTTC 
TCCATCTACT GTGGCATCAG CATTGCCCAT TCCCTCCTAT ACAAGAAAGT GAATGAGGCG 
CAGTATCGCA GCCACCTTGC CAATGAGATG ATGATGTACC ACATGAAGGT CTCTGATGAC 
GAGTACACCA AACTTCTCCA TGACGGGATC CAGCCTGTGG CTGCCATCGA CTCCAACTTT 
GCCAGTTTCA CATACACTCC TCGCTCTCTG CCCGAGGATG ACACTTCCAT GGCCATCCTG 
AGCATGCTGC AGGACATGAA TTTCATCAAT AACTACAAAA TTGACTGCCC GACACTGGCC 
CGGTTCTGTT TGATGGTGAA GAAGGGCTAC CGGGATCCCC CCTACCACAA CTGGATGCAC 
GCCTTTTCTG TCTCCCACTT CTGCTACCTG CTCTACAAGA ACCTGGAGCT CACCAACTAC 
CTCGAGGACA TGGAGATCTT TGCCTTGTTT ATTTCCTGCA TGTGTCACGA CCTGGACCAC 
AGAGGCACAA ACAACTCCTT CCAGGTGGCC TCGAAATCTG TGCTGGCCGC GCTCTACAGC 
TCGGAAGGCT CTGTCATGGA GAGGCACCAC TTCGCTCAGG CCATTGCCAT CCTCAACACC 
CACGGCTGCA ACATCTTTGA CCACTTCTCC CGGAAGGATT ATCAGCGCAT GTTGGACCTG 
ATGCGGGACA TCATCTTGGC CACAGATCTG GCCCACCACC TCCGCATCTT CAAGGACCTC 
CAAAAGATGG CCGAAGTGGG CTATGATCGA ACCAACAAGC AGCACCACAG CCTCCTTCTC 
TGCCTCCTTA TGACCTCCTG TGACCTCTCT GACCAGACCA AGGGCTGGAA GACCACGAGG 
AAGATCGCGG AGCTGATCTA CAAAGAGTTC TTCTCCCAGG GAGACTTGGA GAAGGCCATG 
GGCAACAGGC CGATGGAGAT GATGGACCGT GAGAAGGCCT ACATCCCCGA GCTGCAGATC 
AGCTTCATGG AGCACATCGC AATGCCCATC TACAAGCTGC TGCAAGACCT GTTCCCCAAG 
GCGGCCGAGT TGTACGAACG CGTGGCCTCT AATCGTGAGC ACTGGACCAA GGTGTCACAC 
AAGTTCACCA TCCGAGGCCT CCCGAGCAAC AACTCGTTGG ACTTCCTGGA CGAGGAGTAT 
GAGGTGCCTG ACCTGGATGG CGCTAGGGCT CCCATCAATG GCTGTTGCAG CCTTGATGCT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830788.1
CDS	74..2839
Translation	MRRQPAASRDLFAQEPVPPGSGDGALQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTDQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	XM_024975020.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975020.1

ORF Insert Sequence:

ATGCGCCGAC AGCCTGCAGC CAGCCGGGAC CTCTTTGCAC AGGAGCCAGT GCCCCCAGGG 
AGTGGAGACG GCGCATTGCA GGATGCTTTG CTGAGCCTGG GCTCCGTCAT CGACGTTGCA 
GGCTTGCAAC AGGCTGTCAA GGAGGCCCTG TCGGCTGTGC TTCCCAAAGT GGAGACGGTC 
TACACCTACC TGCTGGATGG GGAATCCCGG CTGGTGTGTG AGGAGCCCCC CCACGAGCTG 
CCCCAGGAGG GGAAAGTGCG AGAGGCTGTG ATCTCCCGGA AGCGGCTGGG CTGCAATGGA 
CTGGGCCCCT CAGACCTGCC TGGGAAGCCC TTGGCAAGGC TGGTGGCTCC ACTGGCTCCT 
GACACCCAAG TGCTGGTCAT ACCGCTGGTG GACAAGGAGG CCGGGGCTGT GGCAGCTGTC 
ATCTTGGTGC ACTGTGGTCA GCTGAGTGAC AATGAGGAGT GGAGCCTGCA AGCTGTGGAG 
AAGCATACCC TGGTGGCCCT GAAAAGGGTG CAGGCCTTGC AGCAGCGCGA GTCCAGCGTG 
GCCCCGGAAG CGACCCAGAA TCCTCCGGAG GAGGCAGCGG GAGACCAGAA GGGTGGGGTC 
GCATACACAG ACCAAGACCG AAAGATCCTG CAGCTTTGCG GGGAGCTCTA CGACCTGGAT 
GCATCTTCCC TGCAGCTCAA AGTCCTCCAA TATCTGCAAC AGGAGACCCA GGCATCCCGC 
TGCTGCCTGC TGCTGGTATC CGAGGACAAT CTTCAGCTCT CCTGCAAGGT CATTGGAGAT 
AAAGTACTGG AGGAAGAGAT CAGCTTTCCG TTGACCACAG GACGCCTGGG CCAAGTGGTG 
GAAGACAAGA AGTCTATCCA GCTGAAAGAT CTCACCTCCG AGGATATGCA ACAGCTGCAA 
AGCATGTTGG GCTGTGAGGT GCAGGCCATG CTCTGTGTCC CTGTCATCAG CCGGGCCACT 
GACCAGGTCG TGGCCCTGGC CTGTGCCTTC AACAAGCTCG GAGGAGACTT GTTCACAGAC 
CAGGACGAGC ACGTGATCCA GCACTGCTTC CACTACACCA GCACAGTGCT CACCAGCACC 
CTGGCCTTCC AGAAGGAGCA GAAGCTCAAG TGTGAGTGCC AGGCTCTTCT CCAAGTGGCG 
AAGAACCTCT TCACTCATCT GGATGACGTC TCCGTGCTGC TCCAGGAGAT CATCACAGAG 
GCCAGGAACC TCAGCAATGC TGAGATCTGC TCTGTGTTCC TGCTGGATCA GAACGAGCTG 
GTGGCCAAGG TGTTCGATGG GGGTGTGGTG GAAGATGAGA GCTATGAGAT CCGCATTCCC 
GCTGACCAGG GCATCGCGGG TCATGTGGCG ACCACCGGCC AGATCCTAAA CATCCCAGAT 
GCTTACGCAC ATCCGCTTTT CTACCGAGGC GTGGACGACA GCACCGGCTT CCGGACGCGC 
AACATCCTCT GCTTCCCCAT CAAGAACGAG AACCAGGAGG TCATCGGTGT GGCCGAGCTG 
GTGAACAAGA TCAATGGACC ATGGTTCAGC AAGTTTGATG AAGACCTGGC TACAGCCTTC 
TCCATCTACT GTGGCATCAG CATTGCCCAT TCCCTCCTAT ACAAGAAAGT GAATGAGGCG 
CAGTATCGCA GCCACCTTGC CAATGAGATG ATGATGTACC ACATGAAGGT CTCTGATGAC 
GAGTACACCA AACTTCTCCA TGACGGGATC CAGCCTGTGG CTGCCATCGA CTCCAACTTT 
GCCAGTTTCA CATACACTCC TCGCTCTCTG CCCGAGGATG ACACTTCCAT GGCCATCCTG 
AGCATGCTGC AGGACATGAA TTTCATCAAT AACTACAAAA TTGACTGCCC GACACTGGCC 
CGGTTCTGTT TGATGGTGAA GAAGGGCTAC CGGGATCCCC CCTACCACAA CTGGATGCAC 
GCCTTTTCTG TCTCCCACTT CTGCTACCTG CTCTACAAGA ACCTGGAGCT CACCAACTAC 
CTCGAGGACA TGGAGATCTT TGCCTTGTTT ATTTCCTGCA TGTGTCACGA CCTGGACCAC 
AGAGGCACAA ACAACTCCTT CCAGGTGGCC TCGAAATCTG TGCTGGCCGC GCTCTACAGC 
TCGGAAGGCT CTGTCATGGA GAGGCACCAC TTCGCTCAGG CCATTGCCAT CCTCAACACC 
CACGGCTGCA ACATCTTTGA CCACTTCTCC CGGAAGGATT ATCAGCGCAT GTTGGACCTG 
ATGCGGGACA TCATCTTGGC CACAGATCTG GCCCACCACC TCCGCATCTT CAAGGACCTC 
CAAAAGATGG CCGAAGTGGG CTATGATCGA ACCAACAAGC AGCACCACAG CCTCCTTCTC 
TGCCTCCTTA TGACCTCCTG TGACCTCTCT GACCAGACCA AGGGCTGGAA GACCACGAGG 
AAGATCGCGG AGCTGATCTA CAAAGAGTTC TTCTCCCAGG GAGACTTGGA GAAGGCCATG 
GGCAACAGGC CGATGGAGAT GATGGACCGT GAGAAGGCCT ACATCCCCGA GCTGCAGATC 
AGCTTCATGG AGCACATCGC AATGCCCATC TACAAGCTGC TGCAAGACCT GTTCCCCAAG 
GCGGCCGAGT TGTACGAACG CGTGGCCTCT AATCGTGAGC ACTGGACCAA GGTGTCACAC 
AAGTTCACCA TCCGAGGCCT CCCGAGCAAC AACTCGTTGG ACTTCCTGGA CGAGGAGTAT 
GAGGTGCCTG ACCTGGATGG CGCTAGGGCT CCCATCAATG GCTGTTGCAG CCTTGATGCT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE2A ( XM_024975017.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830785.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222653
Clone ID Related Accession (Same CDS sequence)	XM_024975017.1
Accession Version	XM_024975017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACATG CAAAGACCTT GAGGCGGGGC CAGCAGGTCT TCCTCAAGCC GGACGAGCCG 
CCGCCGCCGC CGCAGCCATG CGCCGACAGC CTGCAGCCAG CCGGGACCTC TTTGCACAGG 
AGCCAGTGCC CCCAGGGAGT GGAGACGGCG CATTGCAGTC CATGCGCCGG GAATTGGGGA 
ACAGCTGAGA TGGACCAGAC CCTGAAGACA GAGCCAAATA CAGTCATGGG ACCCAGCAGT 
GAGGCTGTGG GAGAGACGCA TGCCAGAGAA GAACAGACTG AGAGAGAGAG ACAGACGTGG 
AGAATCCAGG AAGGCCGGGA GGGAAACAGA GTTCAGGATG CTTTGCTGAG CCTGGGCTCC 
GTCATCGACG TTGCAGGCTT GCAACAGGCT GTCAAGGAGG CCCTGTCGGC TGTGCTTCCC 
AAAGTGGAGA CGGTCTACAC CTACCTGCTG GATGGGGAAT CCCGGCTGGT GTGTGAGGAG 
CCCCCCCACG AGCTGCCCCA GGAGGGGAAA GTGCGAGAGG CTGTGATCTC CCGGAAGCGG 
CTGGGCTGCA ATGGACTGGG CCCCTCAGAC CTGCCTGGGA AGCCCTTGGC AAGGCTGGTG 
GCTCCACTGG CTCCTGACAC CCAAGTGCTG GTCATACCGC TGGTGGACAA GGAGGCCGGG 
GCTGTGGCAG CTGTCATCTT GGTGCACTGT GGTCAGCTGA GTGACAATGA GGAGTGGAGC 
CTGCAAGCTG TGGAGAAGCA TACCCTGGTG GCCCTGAAAA GGGTGCAGGC CTTGCAGCAG 
CGCGAGTCCA GCGTGGCCCC GGAAGCGACC CAGAATCCTC CGGAGGAGGC AGCGGGAGAC 
CAGAAGGGTG GGGTCGCATA CACAGACCAA GACCGAAAGA TCCTGCAGCT TTGCGGGGAG 
CTCTACGACC TGGATGCATC TTCCCTGCAG CTCAAAGTCC TCCAATATCT GCAACAGGAG 
ACCCAGGCAT CCCGCTGCTG CCTGCTGCTG GTATCCGAGG ACAATCTTCA GCTCTCCTGC 
AAGGTCATTG GAGATAAAGT ACTGGAGGAA GAGATCAGCT TTCCGTTGAC CACAGGACGC 
CTGGGCCAAG TGGTGGAAGA CAAGAAGTCT ATCCAGCTGA AAGATCTCAC CTCCGAGGAT 
ATGCAACAGC TGCAAAGCAT GTTGGGCTGT GAGGTGCAGG CCATGCTCTG TGTCCCTGTC 
ATCAGCCGGG CCACTGACCA GGTCGTGGCC CTGGCCTGTG CCTTCAACAA GCTCGGAGGA 
GACTTGTTCA CAGACCAGGA CGAGCACGTG ATCCAGCACT GCTTCCACTA CACCAGCACA 
GTGCTCACCA GCACCCTGGC CTTCCAGAAG GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT 
CTTCTCCAAG TGGCGAAGAA CCTCTTCACT CATCTGGATG ACGTCTCCGT GCTGCTCCAG 
GAGATCATCA CAGAGGCCAG GAACCTCAGC AATGCTGAGA TCTGCTCTGT GTTCCTGCTG 
GATCAGAACG AGCTGGTGGC CAAGGTGTTC GATGGGGGTG TGGTGGAAGA TGAGAGCTAT 
GAGATCCGCA TTCCCGCTGA CCAGGGCATC GCGGGTCATG TGGCGACCAC CGGCCAGATC 
CTAAACATCC CAGATGCTTA CGCACATCCG CTTTTCTACC GAGGCGTGGA CGACAGCACC 
GGCTTCCGGA CGCGCAACAT CCTCTGCTTC CCCATCAAGA ACGAGAACCA GGAGGTCATC 
GGTGTGGCCG AGCTGGTGAA CAAGATCAAT GGACCATGGT TCAGCAAGTT TGATGAAGAC 
CTGGCTACAG CCTTCTCCAT CTACTGTGGC ATCAGCATTG CCCATTCCCT CCTATACAAG 
AAAGTGAATG AGGCGCAGTA TCGCAGCCAC CTTGCCAATG AGATGATGAT GTACCACATG 
AAGGTCTCTG ATGACGAGTA CACCAAACTT CTCCATGACG GGATCCAGCC TGTGGCTGCC 
ATCGACTCCA ACTTTGCCAG TTTCACATAC ACTCCTCGCT CTCTGCCCGA GGATGACACT 
TCCATGGCCA TCCTGAGCAT GCTGCAGGAC ATGAATTTCA TCAATAACTA CAAAATTGAC 
TGCCCGACAC TGGCCCGGTT CTGTTTGATG GTGAAGAAGG GCTACCGGGA TCCCCCCTAC 
CACAACTGGA TGCACGCCTT TTCTGTCTCC CACTTCTGCT ACCTGCTCTA CAAGAACCTG 
GAGCTCACCA ACTACCTCGA GGACATGGAG ATCTTTGCCT TGTTTATTTC CTGCATGTGT 
CACGACCTGG ACCACAGAGG CACAAACAAC TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG 
GCCGCGCTCT ACAGCTCGGA AGGCTCTGTC ATGGAGAGGC ACCACTTCGC TCAGGCCATT 
GCCATCCTCA ACACCCACGG CTGCAACATC TTTGACCACT TCTCCCGGAA GGATTATCAG 
CGCATGTTGG ACCTGATGCG GGACATCATC TTGGCCACAG ATCTGGCCCA CCACCTCCGC 
ATCTTCAAGG ACCTCCAAAA GATGGCCGAA GTGGGCTATG ATCGAACCAA CAAGCAGCAC 
CACAGCCTCC TTCTCTGCCT CCTTATGACC TCCTGTGACC TCTCTGACCA GACCAAGGGC 
TGGAAGACCA CGAGGAAGAT CGCGGAGCTG ATCTACAAAG AGTTCTTCTC CCAGGGAGAC 
TTGGAGAAGG CCATGGGCAA CAGGCCGATG GAGATGATGG ACCGTGAGAA GGCCTACATC 
CCCGAGCTGC AGATCAGCTT CATGGAGCAC ATCGCAATGC CCATCTACAA GCTGCTGCAA 
GACCTGTTCC CCAAGGCGGC CGAGTTGTAC GAACGCGTGG CCTCTAATCG TGAGCACTGG 
ACCAAGGTGT CACACAAGTT CACCATCCGA GGCCTCCCGA GCAACAACTC GTTGGACTTC 
CTGGACGAGG AGTATGAGGT GCCTGACCTG GATGGCGCTA GGGCTCCCAT CAATGGCTGT 
TGCAGCCTTG ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830785.1
CDS	189..3209
Translation	MPHAKTLRRGQQVFLKPDEPPPPPQPCADSLQPAGTSLHRSQCPQGVETAHCSPCAGNWGTAEMDQTLKTEPNTVMGPSSEAVGETHAREEQTERERQTWRIQEGREGNRVQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTDQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	XM_024975017.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975017.1

ORF Insert Sequence:

ATGCCACATG CAAAGACCTT GAGGCGGGGC CAGCAGGTCT TCCTCAAGCC GGACGAGCCG 
CCGCCGCCGC CGCAGCCATG CGCCGACAGC CTGCAGCCAG CCGGGACCTC TTTGCACAGG 
AGCCAGTGCC CCCAGGGAGT GGAGACGGCG CATTGCAGTC CATGCGCCGG GAATTGGGGA 
ACAGCTGAGA TGGACCAGAC CCTGAAGACA GAGCCAAATA CAGTCATGGG ACCCAGCAGT 
GAGGCTGTGG GAGAGACGCA TGCCAGAGAA GAACAGACTG AGAGAGAGAG ACAGACGTGG 
AGAATCCAGG AAGGCCGGGA GGGAAACAGA GTTCAGGATG CTTTGCTGAG CCTGGGCTCC 
GTCATCGACG TTGCAGGCTT GCAACAGGCT GTCAAGGAGG CCCTGTCGGC TGTGCTTCCC 
AAAGTGGAGA CGGTCTACAC CTACCTGCTG GATGGGGAAT CCCGGCTGGT GTGTGAGGAG 
CCCCCCCACG AGCTGCCCCA GGAGGGGAAA GTGCGAGAGG CTGTGATCTC CCGGAAGCGG 
CTGGGCTGCA ATGGACTGGG CCCCTCAGAC CTGCCTGGGA AGCCCTTGGC AAGGCTGGTG 
GCTCCACTGG CTCCTGACAC CCAAGTGCTG GTCATACCGC TGGTGGACAA GGAGGCCGGG 
GCTGTGGCAG CTGTCATCTT GGTGCACTGT GGTCAGCTGA GTGACAATGA GGAGTGGAGC 
CTGCAAGCTG TGGAGAAGCA TACCCTGGTG GCCCTGAAAA GGGTGCAGGC CTTGCAGCAG 
CGCGAGTCCA GCGTGGCCCC GGAAGCGACC CAGAATCCTC CGGAGGAGGC AGCGGGAGAC 
CAGAAGGGTG GGGTCGCATA CACAGACCAA GACCGAAAGA TCCTGCAGCT TTGCGGGGAG 
CTCTACGACC TGGATGCATC TTCCCTGCAG CTCAAAGTCC TCCAATATCT GCAACAGGAG 
ACCCAGGCAT CCCGCTGCTG CCTGCTGCTG GTATCCGAGG ACAATCTTCA GCTCTCCTGC 
AAGGTCATTG GAGATAAAGT ACTGGAGGAA GAGATCAGCT TTCCGTTGAC CACAGGACGC 
CTGGGCCAAG TGGTGGAAGA CAAGAAGTCT ATCCAGCTGA AAGATCTCAC CTCCGAGGAT 
ATGCAACAGC TGCAAAGCAT GTTGGGCTGT GAGGTGCAGG CCATGCTCTG TGTCCCTGTC 
ATCAGCCGGG CCACTGACCA GGTCGTGGCC CTGGCCTGTG CCTTCAACAA GCTCGGAGGA 
GACTTGTTCA CAGACCAGGA CGAGCACGTG ATCCAGCACT GCTTCCACTA CACCAGCACA 
GTGCTCACCA GCACCCTGGC CTTCCAGAAG GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT 
CTTCTCCAAG TGGCGAAGAA CCTCTTCACT CATCTGGATG ACGTCTCCGT GCTGCTCCAG 
GAGATCATCA CAGAGGCCAG GAACCTCAGC AATGCTGAGA TCTGCTCTGT GTTCCTGCTG 
GATCAGAACG AGCTGGTGGC CAAGGTGTTC GATGGGGGTG TGGTGGAAGA TGAGAGCTAT 
GAGATCCGCA TTCCCGCTGA CCAGGGCATC GCGGGTCATG TGGCGACCAC CGGCCAGATC 
CTAAACATCC CAGATGCTTA CGCACATCCG CTTTTCTACC GAGGCGTGGA CGACAGCACC 
GGCTTCCGGA CGCGCAACAT CCTCTGCTTC CCCATCAAGA ACGAGAACCA GGAGGTCATC 
GGTGTGGCCG AGCTGGTGAA CAAGATCAAT GGACCATGGT TCAGCAAGTT TGATGAAGAC 
CTGGCTACAG CCTTCTCCAT CTACTGTGGC ATCAGCATTG CCCATTCCCT CCTATACAAG 
AAAGTGAATG AGGCGCAGTA TCGCAGCCAC CTTGCCAATG AGATGATGAT GTACCACATG 
AAGGTCTCTG ATGACGAGTA CACCAAACTT CTCCATGACG GGATCCAGCC TGTGGCTGCC 
ATCGACTCCA ACTTTGCCAG TTTCACATAC ACTCCTCGCT CTCTGCCCGA GGATGACACT 
TCCATGGCCA TCCTGAGCAT GCTGCAGGAC ATGAATTTCA TCAATAACTA CAAAATTGAC 
TGCCCGACAC TGGCCCGGTT CTGTTTGATG GTGAAGAAGG GCTACCGGGA TCCCCCCTAC 
CACAACTGGA TGCACGCCTT TTCTGTCTCC CACTTCTGCT ACCTGCTCTA CAAGAACCTG 
GAGCTCACCA ACTACCTCGA GGACATGGAG ATCTTTGCCT TGTTTATTTC CTGCATGTGT 
CACGACCTGG ACCACAGAGG CACAAACAAC TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG 
GCCGCGCTCT ACAGCTCGGA AGGCTCTGTC ATGGAGAGGC ACCACTTCGC TCAGGCCATT 
GCCATCCTCA ACACCCACGG CTGCAACATC TTTGACCACT TCTCCCGGAA GGATTATCAG 
CGCATGTTGG ACCTGATGCG GGACATCATC TTGGCCACAG ATCTGGCCCA CCACCTCCGC 
ATCTTCAAGG ACCTCCAAAA GATGGCCGAA GTGGGCTATG ATCGAACCAA CAAGCAGCAC 
CACAGCCTCC TTCTCTGCCT CCTTATGACC TCCTGTGACC TCTCTGACCA GACCAAGGGC 
TGGAAGACCA CGAGGAAGAT CGCGGAGCTG ATCTACAAAG AGTTCTTCTC CCAGGGAGAC 
TTGGAGAAGG CCATGGGCAA CAGGCCGATG GAGATGATGG ACCGTGAGAA GGCCTACATC 
CCCGAGCTGC AGATCAGCTT CATGGAGCAC ATCGCAATGC CCATCTACAA GCTGCTGCAA 
GACCTGTTCC CCAAGGCGGC CGAGTTGTAC GAACGCGTGG CCTCTAATCG TGAGCACTGG 
ACCAAGGTGT CACACAAGTT CACCATCCGA GGCCTCCCGA GCAACAACTC GTTGGACTTC 
CTGGACGAGG AGTATGAGGT GCCTGACCTG GATGGCGCTA GGGCTCCCAT CAATGGCTGT 
TGCAGCCTTG ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE2A ( XM_024975018.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830786.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222654
Clone ID Related Accession (Same CDS sequence)	XM_024975018.1
Accession Version	XM_024975018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAGCCGCC GCCGCCGCCG 
CAGCCATGCG CCGACAGCCT GCAGCCAGCC GGGACCTCTT TGCACAGGAG CCAGTGCCCC 
CAGGGAGTGG AGACGGCGCA TTGCAGTCCA TGCGCCGGGA ATTGGGGAAC AGCTGAGATG 
GACCAGACCC TGAAGACAGA GCCAAATACA GTCATGGGAC CCAGCAGTGA GGCTGTGGGA 
GAGACGCATG CCAGAGAAGA ACAGACTGAG AGAGAGAGAC AGACGTGGAG AATCCAGGAA 
GGCCGGGAGG GAAACAGAGT TCAGGATGCT TTGCTGAGCC TGGGCTCCGT CATCGACGTT 
GCAGGCTTGC AACAGGCTGT CAAGGAGGCC CTGTCGGCTG TGCTTCCCAA AGTGGAGACG 
GTCTACACCT ACCTGCTGGA TGGGGAATCC CGGCTGGTGT GTGAGGAGCC CCCCCACGAG 
CTGCCCCAGG AGGGGAAAGT GCGAGAGGCT GTGATCTCCC GGAAGCGGCT GGGCTGCAAT 
GGACTGGGCC CCTCAGACCT GCCTGGGAAG CCCTTGGCAA GGCTGGTGGC TCCACTGGCT 
CCTGACACCC AAGTGCTGGT CATACCGCTG GTGGACAAGG AGGCCGGGGC TGTGGCAGCT 
GTCATCTTGG TGCACTGTGG TCAGCTGAGT GACAATGAGG AGTGGAGCCT GCAAGCTGTG 
GAGAAGCATA CCCTGGTGGC CCTGAAAAGG GTGCAGGCCT TGCAGCAGCG CGAGTCCAGC 
GTGGCCCCGG AAGCGACCCA GAATCCTCCG GAGGAGGCAG CGGGAGACCA GAAGGGTGGG 
GTCGCATACA CAGACCAAGA CCGAAAGATC CTGCAGCTTT GCGGGGAGCT CTACGACCTG 
GATGCATCTT CCCTGCAGCT CAAAGTCCTC CAATATCTGC AACAGGAGAC CCAGGCATCC 
CGCTGCTGCC TGCTGCTGGT ATCCGAGGAC AATCTTCAGC TCTCCTGCAA GGTCATTGGA 
GATAAAGTAC TGGAGGAAGA GATCAGCTTT CCGTTGACCA CAGGACGCCT GGGCCAAGTG 
GTGGAAGACA AGAAGTCTAT CCAGCTGAAA GATCTCACCT CCGAGGATAT GCAACAGCTG 
CAAAGCATGT TGGGCTGTGA GGTGCAGGCC ATGCTCTGTG TCCCTGTCAT CAGCCGGGCC 
ACTGACCAGG TCGTGGCCCT GGCCTGTGCC TTCAACAAGC TCGGAGGAGA CTTGTTCACA 
GACCAGGACG AGCACGTGAT CCAGCACTGC TTCCACTACA CCAGCACAGT GCTCACCAGC 
ACCCTGGCCT TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG 
GCGAAGAACC TCTTCACTCA TCTGGATGAC GTCTCCGTGC TGCTCCAGGA GATCATCACA 
GAGGCCAGGA ACCTCAGCAA TGCTGAGATC TGCTCTGTGT TCCTGCTGGA TCAGAACGAG 
CTGGTGGCCA AGGTGTTCGA TGGGGGTGTG GTGGAAGATG AGAGCTATGA GATCCGCATT 
CCCGCTGACC AGGGCATCGC GGGTCATGTG GCGACCACCG GCCAGATCCT AAACATCCCA 
GATGCTTACG CACATCCGCT TTTCTACCGA GGCGTGGACG ACAGCACCGG CTTCCGGACG 
CGCAACATCC TCTGCTTCCC CATCAAGAAC GAGAACCAGG AGGTCATCGG TGTGGCCGAG 
CTGGTGAACA AGATCAATGG ACCATGGTTC AGCAAGTTTG ATGAAGACCT GGCTACAGCC 
TTCTCCATCT ACTGTGGCAT CAGCATTGCC CATTCCCTCC TATACAAGAA AGTGAATGAG 
GCGCAGTATC GCAGCCACCT TGCCAATGAG ATGATGATGT ACCACATGAA GGTCTCTGAT 
GACGAGTACA CCAAACTTCT CCATGACGGG ATCCAGCCTG TGGCTGCCAT CGACTCCAAC 
TTTGCCAGTT TCACATACAC TCCTCGCTCT CTGCCCGAGG ATGACACTTC CATGGCCATC 
CTGAGCATGC TGCAGGACAT GAATTTCATC AATAACTACA AAATTGACTG CCCGACACTG 
GCCCGGTTCT GTTTGATGGT GAAGAAGGGC TACCGGGATC CCCCCTACCA CAACTGGATG 
CACGCCTTTT CTGTCTCCCA CTTCTGCTAC CTGCTCTACA AGAACCTGGA GCTCACCAAC 
TACCTCGAGG ACATGGAGAT CTTTGCCTTG TTTATTTCCT GCATGTGTCA CGACCTGGAC 
CACAGAGGCA CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC CGCGCTCTAC 
AGCTCGGAAG GCTCTGTCAT GGAGAGGCAC CACTTCGCTC AGGCCATTGC CATCCTCAAC 
ACCCACGGCT GCAACATCTT TGACCACTTC TCCCGGAAGG ATTATCAGCG CATGTTGGAC 
CTGATGCGGG ACATCATCTT GGCCACAGAT CTGGCCCACC ACCTCCGCAT CTTCAAGGAC 
CTCCAAAAGA TGGCCGAAGT GGGCTATGAT CGAACCAACA AGCAGCACCA CAGCCTCCTT 
CTCTGCCTCC TTATGACCTC CTGTGACCTC TCTGACCAGA CCAAGGGCTG GAAGACCACG 
AGGAAGATCG CGGAGCTGAT CTACAAAGAG TTCTTCTCCC AGGGAGACTT GGAGAAGGCC 
ATGGGCAACA GGCCGATGGA GATGATGGAC CGTGAGAAGG CCTACATCCC CGAGCTGCAG 
ATCAGCTTCA TGGAGCACAT CGCAATGCCC ATCTACAAGC TGCTGCAAGA CCTGTTCCCC 
AAGGCGGCCG AGTTGTACGA ACGCGTGGCC TCTAATCGTG AGCACTGGAC CAAGGTGTCA 
CACAAGTTCA CCATCCGAGG CCTCCCGAGC AACAACTCGT TGGACTTCCT GGACGAGGAG 
TATGAGGTGC CTGACCTGGA TGGCGCTAGG GCTCCCATCA ATGGCTGTTG CAGCCTTGAT 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830786.1
CDS	201..3269
Translation	MGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQPAGTSLHRSQCPQGVETAHCSPCAGNWGTAEMDQTLKTEPNTVMGPSSEAVGETHAREEQTERERQTWRIQEGREGNRVQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTDQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	XM_024975018.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975018.1

ORF Insert Sequence:

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAGCCGCC GCCGCCGCCG 
CAGCCATGCG CCGACAGCCT GCAGCCAGCC GGGACCTCTT TGCACAGGAG CCAGTGCCCC 
CAGGGAGTGG AGACGGCGCA TTGCAGTCCA TGCGCCGGGA ATTGGGGAAC AGCTGAGATG 
GACCAGACCC TGAAGACAGA GCCAAATACA GTCATGGGAC CCAGCAGTGA GGCTGTGGGA 
GAGACGCATG CCAGAGAAGA ACAGACTGAG AGAGAGAGAC AGACGTGGAG AATCCAGGAA 
GGCCGGGAGG GAAACAGAGT TCAGGATGCT TTGCTGAGCC TGGGCTCCGT CATCGACGTT 
GCAGGCTTGC AACAGGCTGT CAAGGAGGCC CTGTCGGCTG TGCTTCCCAA AGTGGAGACG 
GTCTACACCT ACCTGCTGGA TGGGGAATCC CGGCTGGTGT GTGAGGAGCC CCCCCACGAG 
CTGCCCCAGG AGGGGAAAGT GCGAGAGGCT GTGATCTCCC GGAAGCGGCT GGGCTGCAAT 
GGACTGGGCC CCTCAGACCT GCCTGGGAAG CCCTTGGCAA GGCTGGTGGC TCCACTGGCT 
CCTGACACCC AAGTGCTGGT CATACCGCTG GTGGACAAGG AGGCCGGGGC TGTGGCAGCT 
GTCATCTTGG TGCACTGTGG TCAGCTGAGT GACAATGAGG AGTGGAGCCT GCAAGCTGTG 
GAGAAGCATA CCCTGGTGGC CCTGAAAAGG GTGCAGGCCT TGCAGCAGCG CGAGTCCAGC 
GTGGCCCCGG AAGCGACCCA GAATCCTCCG GAGGAGGCAG CGGGAGACCA GAAGGGTGGG 
GTCGCATACA CAGACCAAGA CCGAAAGATC CTGCAGCTTT GCGGGGAGCT CTACGACCTG 
GATGCATCTT CCCTGCAGCT CAAAGTCCTC CAATATCTGC AACAGGAGAC CCAGGCATCC 
CGCTGCTGCC TGCTGCTGGT ATCCGAGGAC AATCTTCAGC TCTCCTGCAA GGTCATTGGA 
GATAAAGTAC TGGAGGAAGA GATCAGCTTT CCGTTGACCA CAGGACGCCT GGGCCAAGTG 
GTGGAAGACA AGAAGTCTAT CCAGCTGAAA GATCTCACCT CCGAGGATAT GCAACAGCTG 
CAAAGCATGT TGGGCTGTGA GGTGCAGGCC ATGCTCTGTG TCCCTGTCAT CAGCCGGGCC 
ACTGACCAGG TCGTGGCCCT GGCCTGTGCC TTCAACAAGC TCGGAGGAGA CTTGTTCACA 
GACCAGGACG AGCACGTGAT CCAGCACTGC TTCCACTACA CCAGCACAGT GCTCACCAGC 
ACCCTGGCCT TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG 
GCGAAGAACC TCTTCACTCA TCTGGATGAC GTCTCCGTGC TGCTCCAGGA GATCATCACA 
GAGGCCAGGA ACCTCAGCAA TGCTGAGATC TGCTCTGTGT TCCTGCTGGA TCAGAACGAG 
CTGGTGGCCA AGGTGTTCGA TGGGGGTGTG GTGGAAGATG AGAGCTATGA GATCCGCATT 
CCCGCTGACC AGGGCATCGC GGGTCATGTG GCGACCACCG GCCAGATCCT AAACATCCCA 
GATGCTTACG CACATCCGCT TTTCTACCGA GGCGTGGACG ACAGCACCGG CTTCCGGACG 
CGCAACATCC TCTGCTTCCC CATCAAGAAC GAGAACCAGG AGGTCATCGG TGTGGCCGAG 
CTGGTGAACA AGATCAATGG ACCATGGTTC AGCAAGTTTG ATGAAGACCT GGCTACAGCC 
TTCTCCATCT ACTGTGGCAT CAGCATTGCC CATTCCCTCC TATACAAGAA AGTGAATGAG 
GCGCAGTATC GCAGCCACCT TGCCAATGAG ATGATGATGT ACCACATGAA GGTCTCTGAT 
GACGAGTACA CCAAACTTCT CCATGACGGG ATCCAGCCTG TGGCTGCCAT CGACTCCAAC 
TTTGCCAGTT TCACATACAC TCCTCGCTCT CTGCCCGAGG ATGACACTTC CATGGCCATC 
CTGAGCATGC TGCAGGACAT GAATTTCATC AATAACTACA AAATTGACTG CCCGACACTG 
GCCCGGTTCT GTTTGATGGT GAAGAAGGGC TACCGGGATC CCCCCTACCA CAACTGGATG 
CACGCCTTTT CTGTCTCCCA CTTCTGCTAC CTGCTCTACA AGAACCTGGA GCTCACCAAC 
TACCTCGAGG ACATGGAGAT CTTTGCCTTG TTTATTTCCT GCATGTGTCA CGACCTGGAC 
CACAGAGGCA CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC CGCGCTCTAC 
AGCTCGGAAG GCTCTGTCAT GGAGAGGCAC CACTTCGCTC AGGCCATTGC CATCCTCAAC 
ACCCACGGCT GCAACATCTT TGACCACTTC TCCCGGAAGG ATTATCAGCG CATGTTGGAC 
CTGATGCGGG ACATCATCTT GGCCACAGAT CTGGCCCACC ACCTCCGCAT CTTCAAGGAC 
CTCCAAAAGA TGGCCGAAGT GGGCTATGAT CGAACCAACA AGCAGCACCA CAGCCTCCTT 
CTCTGCCTCC TTATGACCTC CTGTGACCTC TCTGACCAGA CCAAGGGCTG GAAGACCACG 
AGGAAGATCG CGGAGCTGAT CTACAAAGAG TTCTTCTCCC AGGGAGACTT GGAGAAGGCC 
ATGGGCAACA GGCCGATGGA GATGATGGAC CGTGAGAAGG CCTACATCCC CGAGCTGCAG 
ATCAGCTTCA TGGAGCACAT CGCAATGCCC ATCTACAAGC TGCTGCAAGA CCTGTTCCCC 
AAGGCGGCCG AGTTGTACGA ACGCGTGGCC TCTAATCGTG AGCACTGGAC CAAGGTGTCA 
CACAAGTTCA CCATCCGAGG CCTCCCGAGC AACAACTCGT TGGACTTCCT GGACGAGGAG 
TATGAGGTGC CTGACCTGGA TGGCGCTAGG GCTCCCATCA ATGGCTGTTG CAGCCTTGAT 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE2A ( XM_024975019.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830787.1 Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa222655
Clone ID Related Accession (Same CDS sequence)	XM_024975019.1
Accession Version	XM_024975019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CCCATTCAAT GGATTGCTGC CCAACCAAAC ATGGCAGCCC TGCGGGGAGA 
GGGACAGAGC CAGTGACCAC TTGGAGGGAA GCCTGTGGTC TTTCCTACCT CTGCCAGTGT 
CAGACGAAGA CATTTAGGAG GAGGGGCCTT GGGCTGGCCT TAAAAGGAAG AGTCACTTCT 
CCAGACAGAG CTGAGAGAGA GCTCCAGGAA GAGGGAATGC CACATGCAAA GACCTTGAGG 
CGGGGCCAGC AGGTCTTCCT CAAGCCGGAC GAGCCGCCGC CGCCGCCGCA GCCATGCGCC 
GACAGCCTGC AGGATGCTTT GCTGAGCCTG GGCTCCGTCA TCGACGTTGC AGGCTTGCAA 
CAGGCTGTCA AGGAGGCCCT GTCGGCTGTG CTTCCCAAAG TGGAGACGGT CTACACCTAC 
CTGCTGGATG GGGAATCCCG GCTGGTGTGT GAGGAGCCCC CCCACGAGCT GCCCCAGGAG 
GGGAAAGTGC GAGAGGCTGT GATCTCCCGG AAGCGGCTGG GCTGCAATGG ACTGGGCCCC 
TCAGACCTGC CTGGGAAGCC CTTGGCAAGG CTGGTGGCTC CACTGGCTCC TGACACCCAA 
GTGCTGGTCA TACCGCTGGT GGACAAGGAG GCCGGGGCTG TGGCAGCTGT CATCTTGGTG 
CACTGTGGTC AGCTGAGTGA CAATGAGGAG TGGAGCCTGC AAGCTGTGGA GAAGCATACC 
CTGGTGGCCC TGAAAAGGGT GCAGGCCTTG CAGCAGCGCG AGTCCAGCGT GGCCCCGGAA 
GCGACCCAGA ATCCTCCGGA GGAGGCAGCG GGAGACCAGA AGGGTGGGGT CGCATACACA 
GACCAAGACC GAAAGATCCT GCAGCTTTGC GGGGAGCTCT ACGACCTGGA TGCATCTTCC 
CTGCAGCTCA AAGTCCTCCA ATATCTGCAA CAGGAGACCC AGGCATCCCG CTGCTGCCTG 
CTGCTGGTAT CCGAGGACAA TCTTCAGCTC TCCTGCAAGG TCATTGGAGA TAAAGTACTG 
GAGGAAGAGA TCAGCTTTCC GTTGACCACA GGACGCCTGG GCCAAGTGGT GGAAGACAAG 
AAGTCTATCC AGCTGAAAGA TCTCACCTCC GAGGATATGC AACAGCTGCA AAGCATGTTG 
GGCTGTGAGG TGCAGGCCAT GCTCTGTGTC CCTGTCATCA GCCGGGCCAC TGACCAGGTC 
GTGGCCCTGG CCTGTGCCTT CAACAAGCTC GGAGGAGACT TGTTCACAGA CCAGGACGAG 
CACGTGATCC AGCACTGCTT CCACTACACC AGCACAGTGC TCACCAGCAC CCTGGCCTTC 
CAGAAGGAGC AGAAGCTCAA GTGTGAGTGC CAGGCTCTTC TCCAAGTGGC GAAGAACCTC 
TTCACTCATC TGGATGACGT CTCCGTGCTG CTCCAGGAGA TCATCACAGA GGCCAGGAAC 
CTCAGCAATG CTGAGATCTG CTCTGTGTTC CTGCTGGATC AGAACGAGCT GGTGGCCAAG 
GTGTTCGATG GGGGTGTGGT GGAAGATGAG AGCTATGAGA TCCGCATTCC CGCTGACCAG 
GGCATCGCGG GTCATGTGGC GACCACCGGC CAGATCCTAA ACATCCCAGA TGCTTACGCA 
CATCCGCTTT TCTACCGAGG CGTGGACGAC AGCACCGGCT TCCGGACGCG CAACATCCTC 
TGCTTCCCCA TCAAGAACGA GAACCAGGAG GTCATCGGTG TGGCCGAGCT GGTGAACAAG 
ATCAATGGAC CATGGTTCAG CAAGTTTGAT GAAGACCTGG CTACAGCCTT CTCCATCTAC 
TGTGGCATCA GCATTGCCCA TTCCCTCCTA TACAAGAAAG TGAATGAGGC GCAGTATCGC 
AGCCACCTTG CCAATGAGAT GATGATGTAC CACATGAAGG TCTCTGATGA CGAGTACACC 
AAACTTCTCC ATGACGGGAT CCAGCCTGTG GCTGCCATCG ACTCCAACTT TGCCAGTTTC 
ACATACACTC CTCGCTCTCT GCCCGAGGAT GACACTTCCA TGGCCATCCT GAGCATGCTG 
CAGGACATGA ATTTCATCAA TAACTACAAA ATTGACTGCC CGACACTGGC CCGGTTCTGT 
TTGATGGTGA AGAAGGGCTA CCGGGATCCC CCCTACCACA ACTGGATGCA CGCCTTTTCT 
GTCTCCCACT TCTGCTACCT GCTCTACAAG AACCTGGAGC TCACCAACTA CCTCGAGGAC 
ATGGAGATCT TTGCCTTGTT TATTTCCTGC ATGTGTCACG ACCTGGACCA CAGAGGCACA 
AACAACTCCT TCCAGGTGGC CTCGAAATCT GTGCTGGCCG CGCTCTACAG CTCGGAAGGC 
TCTGTCATGG AGAGGCACCA CTTCGCTCAG GCCATTGCCA TCCTCAACAC CCACGGCTGC 
AACATCTTTG ACCACTTCTC CCGGAAGGAT TATCAGCGCA TGTTGGACCT GATGCGGGAC 
ATCATCTTGG CCACAGATCT GGCCCACCAC CTCCGCATCT TCAAGGACCT CCAAAAGATG 
GCCGAAGTGG GCTATGATCG AACCAACAAG CAGCACCACA GCCTCCTTCT CTGCCTCCTT 
ATGACCTCCT GTGACCTCTC TGACCAGACC AAGGGCTGGA AGACCACGAG GAAGATCGCG 
GAGCTGATCT ACAAAGAGTT CTTCTCCCAG GGAGACTTGG AGAAGGCCAT GGGCAACAGG 
CCGATGGAGA TGATGGACCG TGAGAAGGCC TACATCCCCG AGCTGCAGAT CAGCTTCATG 
GAGCACATCG CAATGCCCAT CTACAAGCTG CTGCAAGACC TGTTCCCCAA GGCGGCCGAG 
TTGTACGAAC GCGTGGCCTC TAATCGTGAG CACTGGACCA AGGTGTCACA CAAGTTCACC 
ATCCGAGGCC TCCCGAGCAA CAACTCGTTG GACTTCCTGG ACGAGGAGTA TGAGGTGCCT 
GACCTGGATG GCGCTAGGGC TCCCATCAAT GGCTGTTGCA GCCTTGATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830787.1
CDS	220..3276
Translation	MGQACGHSILCRSQQYPAARPAEPHSMDCCPTKHGSPAGRGTEPVTTWREACGLSYLCQCQTKTFRRRGLGLALKGRVTSPDRAERELQEEGMPHAKTLRRGQQVFLKPDEPPPPPQPCADSLQDALLSLGSVIDVAGLQQAVKEALSAVLPKVETVYTYLLDGESRLVCEEPPHELPQEGKVREAVISRKRLGCNGLGPSDLPGKPLARLVAPLAPDTQVLVIPLVDKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALKRVQALQQRESSVAPEATQNPPEEAAGDQKGGVAYTDQDRKILQLCGELYDLDASSLQLKVLQYLQQETQASRCCLLLVSEDNLQLSCKVIGDKVLEEEISFPLTTGRLGQVVEDKKSIQLKDLTSEDMQQLQSMLGCEVQAMLCVPVISRATDQVVALACAFNKLGGDLFTDQDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVEDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDMEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRTNKQHHSLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGARAPINGCCSLDAE

Target ORF information:

RefSeq Version	XM_024975019.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 2A (PDE2A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975019.1

ORF Insert Sequence:

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCTGCGCGA 
CCGGCTGAGC CCCATTCAAT GGATTGCTGC CCAACCAAAC ATGGCAGCCC TGCGGGGAGA 
GGGACAGAGC CAGTGACCAC TTGGAGGGAA GCCTGTGGTC TTTCCTACCT CTGCCAGTGT 
CAGACGAAGA CATTTAGGAG GAGGGGCCTT GGGCTGGCCT TAAAAGGAAG AGTCACTTCT 
CCAGACAGAG CTGAGAGAGA GCTCCAGGAA GAGGGAATGC CACATGCAAA GACCTTGAGG 
CGGGGCCAGC AGGTCTTCCT CAAGCCGGAC GAGCCGCCGC CGCCGCCGCA GCCATGCGCC 
GACAGCCTGC AGGATGCTTT GCTGAGCCTG GGCTCCGTCA TCGACGTTGC AGGCTTGCAA 
CAGGCTGTCA AGGAGGCCCT GTCGGCTGTG CTTCCCAAAG TGGAGACGGT CTACACCTAC 
CTGCTGGATG GGGAATCCCG GCTGGTGTGT GAGGAGCCCC CCCACGAGCT GCCCCAGGAG 
GGGAAAGTGC GAGAGGCTGT GATCTCCCGG AAGCGGCTGG GCTGCAATGG ACTGGGCCCC 
TCAGACCTGC CTGGGAAGCC CTTGGCAAGG CTGGTGGCTC CACTGGCTCC TGACACCCAA 
GTGCTGGTCA TACCGCTGGT GGACAAGGAG GCCGGGGCTG TGGCAGCTGT CATCTTGGTG 
CACTGTGGTC AGCTGAGTGA CAATGAGGAG TGGAGCCTGC AAGCTGTGGA GAAGCATACC 
CTGGTGGCCC TGAAAAGGGT GCAGGCCTTG CAGCAGCGCG AGTCCAGCGT GGCCCCGGAA 
GCGACCCAGA ATCCTCCGGA GGAGGCAGCG GGAGACCAGA AGGGTGGGGT CGCATACACA 
GACCAAGACC GAAAGATCCT GCAGCTTTGC GGGGAGCTCT ACGACCTGGA TGCATCTTCC 
CTGCAGCTCA AAGTCCTCCA ATATCTGCAA CAGGAGACCC AGGCATCCCG CTGCTGCCTG 
CTGCTGGTAT CCGAGGACAA TCTTCAGCTC TCCTGCAAGG TCATTGGAGA TAAAGTACTG 
GAGGAAGAGA TCAGCTTTCC GTTGACCACA GGACGCCTGG GCCAAGTGGT GGAAGACAAG 
AAGTCTATCC AGCTGAAAGA TCTCACCTCC GAGGATATGC AACAGCTGCA AAGCATGTTG 
GGCTGTGAGG TGCAGGCCAT GCTCTGTGTC CCTGTCATCA GCCGGGCCAC TGACCAGGTC 
GTGGCCCTGG CCTGTGCCTT CAACAAGCTC GGAGGAGACT TGTTCACAGA CCAGGACGAG 
CACGTGATCC AGCACTGCTT CCACTACACC AGCACAGTGC TCACCAGCAC CCTGGCCTTC 
CAGAAGGAGC AGAAGCTCAA GTGTGAGTGC CAGGCTCTTC TCCAAGTGGC GAAGAACCTC 
TTCACTCATC TGGATGACGT CTCCGTGCTG CTCCAGGAGA TCATCACAGA GGCCAGGAAC 
CTCAGCAATG CTGAGATCTG CTCTGTGTTC CTGCTGGATC AGAACGAGCT GGTGGCCAAG 
GTGTTCGATG GGGGTGTGGT GGAAGATGAG AGCTATGAGA TCCGCATTCC CGCTGACCAG 
GGCATCGCGG GTCATGTGGC GACCACCGGC CAGATCCTAA ACATCCCAGA TGCTTACGCA 
CATCCGCTTT TCTACCGAGG CGTGGACGAC AGCACCGGCT TCCGGACGCG CAACATCCTC 
TGCTTCCCCA TCAAGAACGA GAACCAGGAG GTCATCGGTG TGGCCGAGCT GGTGAACAAG 
ATCAATGGAC CATGGTTCAG CAAGTTTGAT GAAGACCTGG CTACAGCCTT CTCCATCTAC 
TGTGGCATCA GCATTGCCCA TTCCCTCCTA TACAAGAAAG TGAATGAGGC GCAGTATCGC 
AGCCACCTTG CCAATGAGAT GATGATGTAC CACATGAAGG TCTCTGATGA CGAGTACACC 
AAACTTCTCC ATGACGGGAT CCAGCCTGTG GCTGCCATCG ACTCCAACTT TGCCAGTTTC 
ACATACACTC CTCGCTCTCT GCCCGAGGAT GACACTTCCA TGGCCATCCT GAGCATGCTG 
CAGGACATGA ATTTCATCAA TAACTACAAA ATTGACTGCC CGACACTGGC CCGGTTCTGT 
TTGATGGTGA AGAAGGGCTA CCGGGATCCC CCCTACCACA ACTGGATGCA CGCCTTTTCT 
GTCTCCCACT TCTGCTACCT GCTCTACAAG AACCTGGAGC TCACCAACTA CCTCGAGGAC 
ATGGAGATCT TTGCCTTGTT TATTTCCTGC ATGTGTCACG ACCTGGACCA CAGAGGCACA 
AACAACTCCT TCCAGGTGGC CTCGAAATCT GTGCTGGCCG CGCTCTACAG CTCGGAAGGC 
TCTGTCATGG AGAGGCACCA CTTCGCTCAG GCCATTGCCA TCCTCAACAC CCACGGCTGC 
AACATCTTTG ACCACTTCTC CCGGAAGGAT TATCAGCGCA TGTTGGACCT GATGCGGGAC 
ATCATCTTGG CCACAGATCT GGCCCACCAC CTCCGCATCT TCAAGGACCT CCAAAAGATG 
GCCGAAGTGG GCTATGATCG AACCAACAAG CAGCACCACA GCCTCCTTCT CTGCCTCCTT 
ATGACCTCCT GTGACCTCTC TGACCAGACC AAGGGCTGGA AGACCACGAG GAAGATCGCG 
GAGCTGATCT ACAAAGAGTT CTTCTCCCAG GGAGACTTGG AGAAGGCCAT GGGCAACAGG 
CCGATGGAGA TGATGGACCG TGAGAAGGCC TACATCCCCG AGCTGCAGAT CAGCTTCATG 
GAGCACATCG CAATGCCCAT CTACAAGCTG CTGCAAGACC TGTTCCCCAA GGCGGCCGAG 
TTGTACGAAC GCGTGGCCTC TAATCGTGAG CACTGGACCA AGGTGTCACA CAAGTTCACC 
ATCCGAGGCC TCCCGAGCAA CAACTCGTTG GACTTCCTGG ACGAGGAGTA TGAGGTGCCT 
GACCTGGATG GCGCTAGGGC TCCCATCAAT GGCTGTTGCA GCCTTGATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Molecular determinants for cyclic nucleotide binding to the regulatory domains of phosphodiesterase 2A.
The Journal of biological chemistry279(36)37928-38(2004 Sep)
Wu AY,Tang XB,Martinez SE,Ikeda K,Beavo JA

Molecular cloning of a cyclic GMP-stimulated cyclic nucleotide phosphodiesterase cDNA. Identification and distribution of isozyme variants.
The Journal of biological chemistry266(26)17655-61(1991 Sep)
Sonnenburg WK,Mullaney PJ,Beavo JA

Amino acid sequence of the cyclic GMP stimulated cyclic nucleotide phosphodiesterase from bovine heart.
Biochemistry29(44)10280-8(1990 Nov)
Trong HL,Beier N,Sonnenburg WK,Stroop SD,Walsh KA,Beavo JA,Charbonneau H

Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species.
Proceedings of the National Academy of Sciences of the United States of America83(24)9308-12(1986 Dec)
Charbonneau H,Beier N,Walsh KA,Beavo JA

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PDE2A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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