IL10 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL10
Entrez Gene ID	281246
Full Name	interleukin 10
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174088.1	NP_776513.1	interleukin-10 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IL10	NP_000563.1
Canis lupus familiaris (dog)	IL10	NP_001003077.1
Mus musculus (house mouse)	Il10	NP_034678.1
Rattus norvegicus (Norway rat)	Il10	NP_036986.2
Xenopus tropicalis (tropical clawed frog)	il10	NP_001165400.1
	IL10	NP_001038192.1
Bos taurus (cattle)	IL10	NP_776513.1
Gallus gallus (chicken)	IL10	NP_001004414.2
Pan troglodytes (chimpanzee)	IL10	NP_001129092.2
Danio rerio (zebrafish)	il10	NP_001018621.2

Gene Ontology
Bibliography

Related articles in PubMed

An Investigation on the Expression Level of Interleukin 10 (IL-10) in the Healthy and Mastitic Holstein Cows and the Bioinformatics Analysis of Nucleosome Profile.
Varzandian B, Ghaderi-Zefrehei M, Hosseinzadeh S, Sayyadi M, Taghadosi V, Varzandian S
Animal biotechnology28(4)294-300(2017 Oct)

Prostaglandin E2 inhibits IL-10 production by bovine CD4+ T cells.
Zu?ka-Prot M, Ma?lanka T, Jasiecka A, Jaroszewski JJ
Polish journal of veterinary sciences17(3)511-3(2014)

Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.
Shirasuna K, Matsumoto H, Kobayashi E, Nitta A, Haneda S, Matsui M, Kawashima C, Kida K, Shimizu T, Miyamoto A
The Journal of reproduction and development58(1)84-90(2012)

Modulation of human immune responses by bovine interleukin-10.
den Hartog G, Savelkoul HF, Schoemaker R, Tijhaar E, Westphal AH, de Ruiter T, van de Weg-Schrijver E, van Neerven RJ
PloS one6(3)e18188(2011 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10.
Title: Prostaglandin E2 inhibits IL-10 production by bovine CD4+ T cells.

IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes.
Title: Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.

Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation.
Title: Modulation of human immune responses by bovine interleukin-10.

Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses.
Title: Expression of interleukin-10 and suppressor of cytokine signaling-3 associated with susceptibility of cattle to infection with Mycobacterium avium subsp paratuberculosis.

Levels in milk are altered in the presence of bacteria.
Title: Quantitation of the cytokines TNF-alpha, IL-8 and IL-10 in bovine milk using real-time TaqMan PCR.

The following IL10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42372	NM_174088.1 Latest version!	Bos taurus interleukin 10 (IL10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL10 ( NM_174088.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776513.1 Bos taurus interleukin 10 (IL10), mRNA.

Price & Availability↑

CloneID	OBa42372
Clone ID Related Accession (Same CDS sequence)	NM_174088.1
Accession Version	NM_174088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	interleukin-10 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U00799.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U00799.1, CB442949.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145418, SAMN03145419 [ECO:0000350] ##Evidence-Data-END##

ATGCATAGCT CAGCACTACT CTGTTGCCTG GTCTTCCTGG CTGGGGTGGC AGCCAGCCGA 
GATGCGAGCA CCCTGTCTGA CAGCAGCTGT ATCCACTTGC CAACCAGCCT GCCCCACATG 
CTGCGGGAGC TCCGAGCTGC CTTCGGCGAG GCGAAGACTT TCTTTCAAAT GAAGGACCAA 
CTGCACAGCT TACTGTTGAC CCAGTCTCTG CTGGATGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CGGAAATGAT CCAGTTTTAC CTGGAAGAGG TGATGCCACA GGCTGAGAAC 
CACGGGCCTG ACATCAAGGA GCACGTGAAC TCACTGGGGG AGAAGCTGAA GACCCTGCGG 
CTGCGGCTGC GGCGCTGTCA TCGCTTTCTG CCCTGCGAAA ACAAGAGCAA GGCGGTGGAG 
AAGGTGAAGA GAGTCTTCAG TGAGCTCCAA GAGAGGGGTG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAACC TACATGACAA CGAAGATGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776513.1
CDS	1..537
Translation	MHSSALLCCLVFLAGVAASRDASTLSDSSCIHLPTSLPHMLRELRAAFGEAKTFFQMKDQLHSLLLTQSLLDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVEKVKRVFSELQERGVYKAMSEFDIFINYIETYMTTKMQK

Target ORF information:

RefSeq Version	NM_174088.1
Organism	Bos taurus(cattle)
Definition	Bos taurus interleukin 10 (IL10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174088.1

ORF Insert Sequence:

ATGCATAGCT CAGCACTACT CTGTTGCCTG GTCTTCCTGG CTGGGGTGGC AGCCAGCCGA 
GATGCGAGCA CCCTGTCTGA CAGCAGCTGT ATCCACTTGC CAACCAGCCT GCCCCACATG 
CTGCGGGAGC TCCGAGCTGC CTTCGGCGAG GCGAAGACTT TCTTTCAAAT GAAGGACCAA 
CTGCACAGCT TACTGTTGAC CCAGTCTCTG CTGGATGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CGGAAATGAT CCAGTTTTAC CTGGAAGAGG TGATGCCACA GGCTGAGAAC 
CACGGGCCTG ACATCAAGGA GCACGTGAAC TCACTGGGGG AGAAGCTGAA GACCCTGCGG 
CTGCGGCTGC GGCGCTGTCA TCGCTTTCTG CCCTGCGAAA ACAAGAGCAA GGCGGTGGAG 
AAGGTGAAGA GAGTCTTCAG TGAGCTCCAA GAGAGGGGTG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAACC TACATGACAA CGAAGATGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Investigation on the Expression Level of Interleukin 10 (IL-10) in the Healthy and Mastitic Holstein Cows and the Bioinformatics Analysis of Nucleosome Profile.
Animal biotechnology28(4)294-300(2017 Oct)
Varzandian B,Ghaderi-Zefrehei M,Hosseinzadeh S,Sayyadi M,Taghadosi V,Varzandian S

Prostaglandin E2 inhibits IL-10 production by bovine CD4+ T cells.
Polish journal of veterinary sciences17(3)511-3(2014)
Zu?ka-Prot M,Ma?lanka T,Jasiecka A,Jaroszewski JJ

Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.
The Journal of reproduction and development58(1)84-90(2012)
Shirasuna K,Matsumoto H,Kobayashi E,Nitta A,Haneda S,Matsui M,Kawashima C,Kida K,Shimizu T,Miyamoto A

Modulation of human immune responses by bovine interleukin-10.
PloS one6(3)e18188(2011 Mar)
den Hartog G,Savelkoul HF,Schoemaker R,Tijhaar E,Westphal AH,de Ruiter T,van de Weg-Schrijver E,van Neerven RJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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IL10 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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