Il10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il10
Entrez Gene ID	16153
Full Name	interleukin 10
Synonyms	CSIF,Il-10
General protein information	Preferred Names interleukin 10 Names interleukin-10 cytokine synthesis inhibitory factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 56.89 cM
Summary	This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010548.2	NP_034678.1	interleukin-10 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IL10	NP_000563.1
Canis lupus familiaris (dog)	IL10	NP_001003077.1
Mus musculus (house mouse)	Il10	NP_034678.1
Rattus norvegicus (Norway rat)	Il10	NP_036986.2
Xenopus tropicalis (tropical clawed frog)	il10	NP_001165400.1
	IL10	NP_001038192.1
Bos taurus (cattle)	IL10	NP_776513.1
Gallus gallus (chicken)	IL10	NP_001004414.2
Pan troglodytes (chimpanzee)	IL10	NP_001129092.2
Danio rerio (zebrafish)	il10	NP_001018621.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a macrophage-specific chromatin signature in the IL-10 locus.
Saraiva M, Christensen JR, Tsytsykova AV, Goldfeld AE, Ley SC, Kioussis D, O'Garra A
Journal of immunology (Baltimore, Md. : 1950)175(2)1041-6(2005 Jul)

Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.
Fiorentino DF, Bond MW, Mosmann TR
The Journal of experimental medicine170(6)2081-95(1989 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that pretreatment with sodium selenite prevents experimental colitis by restoring interleukin-10 excretion
Title: Dextran sulfate sodium-induced acute experimental colitis in C57BL/6 mice is mitigated by selenium.

FXR activation ameliorated central nervous system autoimmunity in an IL-10-dependent fashion and even suppressed advanced clinical disease upon therapeutic administration
Title: The farnesoid-X-receptor in myeloid cells controls CNS autoimmunity in an IL-10-dependent fashion.

Lactobacillus curvatus WiKim38 induced significantly higher levels of IL-10 in bone marrow-derived dendritic cells.
Title: Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice.

observations demonstrate a critical role for IL-10 in driving mucosal mast cell expansion and activation, suggesting that, in its absence, mast cell function is impaired, leading to attenuated food allergy symptoms.
Title: IL-10 Enhances IgE-Mediated Mast Cell Responses and Is Essential for the Development of Experimental Food Allergy in IL-10-Deficient Mice.

Cisplatin induces immune-suppressive tolerogenic dendritic cells in TLR agonist-induced inflammatory conditions via abundant IL-10 production, thereby skewing Th cell differentiation towards Th2 and Tr1 cells. This relationship may provide cancer cells with an opportunity to evade the immune system.
Title: Cisplatin induces tolerogenic dendritic cells in response to TLR agonists via the abundant production of IL-10, thereby promoting Th2- and Tr1-biased T-cell immunity.

The following Il10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22605	NM_010548.2 Latest version!	Mus musculus interleukin 10 (Il10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il10 ( NM_010548.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034678.1 Mus musculus interleukin 10 (Il10), mRNA.

Price & Availability↑

CloneID	OMu22605
Clone ID Related Accession (Same CDS sequence)	NM_010548.2
Accession Version	NM_010548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	interleukin-10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK152344.1 and AL513351.26. On Mar 26, 2010 this sequence version replaced NM_010548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK152344.1, BC120612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG 
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG 
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC 
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG 
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG 
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG 
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA 
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034678.1
CDS	68..604
Translation	MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS

Target ORF information:

RefSeq Version	NM_010548.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus interleukin 10 (Il10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010548.2

ORF Insert Sequence:

ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG 
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG 
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC 
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG 
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG 
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG 
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA 
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a macrophage-specific chromatin signature in the IL-10 locus.
Journal of immunology (Baltimore, Md. : 1950)175(2)1041-6(2005 Jul)
Saraiva M,Christensen JR,Tsytsykova AV,Goldfeld AE,Ley SC,Kioussis D,O'Garra A

Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.
The Journal of experimental medicine170(6)2081-95(1989 Dec)
Fiorentino DF,Bond MW,Mosmann TR

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Il10 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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