IL10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL10
Entrez Gene ID	3586
Full Name	interleukin 10
Synonyms	CSIF,GVHDS,IL-10,IL10A,TGIF
General protein information	Preferred Names interleukin 10 Names interleukin-10 T-cell growth inhibitory factor cytokine synthesis inhibitory factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011].
Disorder MIM:	124092
Disorder Html:	{HIV-1, susceptibility to}, 609423 (3); {Graft-versus-host disease, protection against}, 614395 (3); {Rheumatoid arthritis, progression of}, 180300 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000572.3	NP_000563.1	interleukin-10 precursor
XM_011509506.1	XP_011507808.1	interleukin-10 isoform X1
NM_000572.2	NP_000563.1	interleukin-10 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IL10	NP_000563.1
Canis lupus familiaris (dog)	IL10	NP_001003077.1
Mus musculus (house mouse)	Il10	NP_034678.1
Rattus norvegicus (Norway rat)	Il10	NP_036986.2
Xenopus tropicalis (tropical clawed frog)	il10	NP_001165400.1
	IL10	NP_001038192.1
Bos taurus (cattle)	IL10	NP_776513.1
Gallus gallus (chicken)	IL10	NP_001004414.2
Pan troglodytes (chimpanzee)	IL10	NP_001129092.2
Danio rerio (zebrafish)	il10	NP_001018621.2

Gene Ontology
Bibliography

Related articles in PubMed

Association of interleukin-10 gene single nucleotide polymorphisms with susceptibility to systemic lupus erythematosus in a Chinese population.
Lv TT, Wu J, Li J, Zhang TP, Yang XK, Xiang N, Fan YG, Pan HF, Wang B
Gene642549-554(2018 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Meta-analysis provides evidence that IL-10 gene rs1800896 polymorphism is associated with the risk of acute pancreatitis.
Title: Interleukin-10 gene rs1800896 polymorphism increases risk of acute pancreatitis.

Although IL-10 SNP frequency has shown heterogeneity in different populations, several studies, including our investigation in a Brazilian cohort, suggest that these polymorphisms, particularly -1082A/G, are an important risk factor for Alzheimer's disease. However, the mechanism in which IL-10 may ameliorate neuroinflammation, cognitive dysfunction or neurodegeneration is not completely clear.
Title: Alzheimer's disease and cytokine IL-10 gene polymorphisms: is there an association?

upon ligation of the T-cell antigen receptor (TCR), the TCR associates with and transactivates CXCR4 via phosphorylation of S339-CXCR4 in order to activate a PREX1-Rac1-signaling pathway that stabilizes interleukin-2(IL-2), IL-4, and IL-10 messenger RNA (mRNA) transcripts.
Title: TCR-CXCR4 signaling stabilizes cytokine mRNA transcripts via a PREX1-Rac1 pathway: implications for CTCL.

expression of interleukin-10 messenger RNA was significantly higher (p < 0.001) by 5.28-fold and 8.95-fold than non-inflammatory breast cancer and healthy control, respectively, where GCC haplotype significantly increased interleukin-10 gene expression (r = 0.9, p < 0.001).
Title: Inflammatory breast cancer: High incidence of GCC haplotypes (-1082A/G, -819T/C, and -592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients' carcinoma tissues.

Inhibition of protein kinase C zeta expression in prostate cancer cells promoted chemotaxis of peripheral macrophages and acquisition of M2 phenotypic features. These results were further supported by the finding that silencing of endogenous protein kinase C zeta promoted the expression of prostate cancer cell-derived interleukin-4 and interleukin-10
Title: PKC? in prostate cancer cells represses the recruitment and M2 polarization of macrophages in the prostate cancer microenvironment.

The following IL10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25319	NM_000572.3 Latest version!	Homo sapiens interleukin 10 (IL10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25319	XM_011509506.1 Latest version!	Homo sapiens interleukin 10 (IL10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25319	NM_000572.2	Homo sapiens interleukin 10 (IL10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL10 ( NM_000572.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000563.1 Homo sapiens interleukin 10 (IL10), mRNA.

Price & Availability↑

CloneID	OHu25319
Clone ID Related Accession (Same CDS sequence)	NM_000572.3 , XM_011509506.1 , NM_000572.2
Accession Version	NM_000572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	interleukin-10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022315.1, M57627.1 and CD369014.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2018 this sequence version replaced NM_000572.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.113941.1, SRR1163655.71990.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000423557.1/ ENSP00000412237.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000563.1
CDS	60..596
Translation	MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Target ORF information:

RefSeq Version	NM_000572.3
Organism	Homo sapiens(human)
Definition	Homo sapiens interleukin 10 (IL10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000572.3

ORF Insert Sequence:

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL10 ( NM_000572.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000563.1 Homo sapiens interleukin 10 (IL10), mRNA.

Price & Availability↑

CloneID	OHu25319
Clone ID Related Accession (Same CDS sequence)	NM_000572.3 , XM_011509506.1 , NM_000572.2
Accession Version	NM_000572.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	interleukin-10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M57627.1 and BC022315.1. This sequence is a reference standard in the RefSeqGene project. On Oct 30, 2002 this sequence version replaced NM_000572.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M57627.1, SRR1163655.71990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000563.1
CDS	60..596
Translation	MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Target ORF information:

RefSeq Version	NM_000572.2
Organism	Homo sapiens(human)
Definition	Homo sapiens interleukin 10 (IL10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000572.2

ORF Insert Sequence:

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL10 ( XM_011509506.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507808.1 Homo sapiens interleukin 10 (IL10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25319
Clone ID Related Accession (Same CDS sequence)	NM_000572.3 , XM_011509506.1 , NM_000572.2
Accession Version	XM_011509506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	interleukin-10 isoform X1
Comment

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507808.1
Translation	MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Target ORF information:

RefSeq Version	XM_011509506.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens interleukin 10 (IL10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509506.1

ORF Insert Sequence:

ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA 
GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG 
CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG 
CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC 
CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG 
CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG 
CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of interleukin-10 gene single nucleotide polymorphisms with susceptibility to systemic lupus erythematosus in a Chinese population.
Gene642549-554(2018 Feb)
Lv TT,Wu J,Li J,Zhang TP,Yang XK,Xiang N,Fan YG,Pan HF,Wang B

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IL10 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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