SCD cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCD
Entrez Gene ID	280924
Full Name	stearoyl-CoA desaturase (delta-9-desaturase)
Synonyms	SCD1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173959.4	NP_776384.3	acyl-CoA desaturase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	desat2	NP_650201.1
Gallus gallus (chicken)	SCD	NP_990221.1
Rattus norvegicus (Norway rat)	Scd1	NP_631931.2
Bos taurus (cattle)	SCD	NP_776384.3
Mus musculus (house mouse)	Scd1	NP_033153.2
Danio rerio (zebrafish)	scdb	NP_001018541.1
Xenopus tropicalis (tropical clawed frog)	scd	NP_001027500.1
Homo sapiens (human)	SCD	NP_005054.3
Pan troglodytes (chimpanzee)	SCD	XP_001168563.1
	SCD	XP_001107910.2
Canis lupus familiaris (dog)	SCD	XP_543968.2

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic mechanisms contribute to decrease stearoyl-CoA desaturase 1 expression in the liver of dairy cows after prolonged feeding of high-concentrate diet.
Xu TL, Seyfert HM, Shen XZ
Journal of dairy science(2017 Dec)

Epistatic effects between pairs of the growth hormone secretagogue receptor 1a, growth hormone, growth hormone receptor, non-SMC condensin I complex, subunit G and stearoyl-CoA desaturase genes on carcass, price-related and fatty acid composition traits in Japanese Black cattle.
Komatsu M, Nishino K, Fujimori Y, Haga Y, Iwama N, Arakawa A, Aihara Y, Takeda H, Takahashi H
Animal science journal = Nihon chikusan Gakkaiho(2017 Nov)

A post-GWAS confirming the SCD gene associated with milk medium- and long-chain unsaturated fatty acids in Chinese Holstein population.
Li C, Sun D, Zhang S, Liu L, Alim MA, Zhang Q
Animal genetics47(4)483-90(2016 Aug)

Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.
Xu T, Shen X, Seyfert HM
BMC molecular biology17(1)16(2016 07)

Milk fatty acid profile is modulated by DGAT1 and SCD1 genotypes in dairy cattle on pasture and strategic supplementation.
Carvajal AM, Huircan P, Dezamour JM, Subiabre I, Kerr B, Morales R, Ungerfeld EM
Genetics and molecular research : GMR15(2)(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

significant effects of the SCD gene on milk medium- and long-chain unsaturated fatty acids, especially for C14:1 and C14 index in dairy cattle
Title: A post-GWAS confirming the SCD gene associated with milk medium- and long-chain unsaturated fatty acids in Chinese Holstein population.

E. coli mastitis reduced SCD1 expression in liver and udder.
Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.

Here, the association between DGAT1 K232A, SCD1 A293V, and LEPR T945M markers with milk fat composition in southern Chile was evaluated. The most frequent variants for DGAT1, SCD1, and LEPR polymorphisms were GC/GC, C, and C, respectively. The SCD1 CC genotype was associated with a low cholesterolemic FA content, a high ratio of linolenic acid to cholesterolemic FA, and lower conjugated-linolenic acid and PUFA content.
Title: Milk fatty acid profile is modulated by DGAT1 and SCD1 genotypes in dairy cattle on pasture and strategic supplementation.

Studied the responsiveness of bovine SCD1 promoter to serum, insulin, oleic acid, and NFY transcription factor in BME-UV1 cells.
Title: Regulation of Stearoyl Coenzyme A Desaturase 1 Gene Promoter in Bovine Mammary Cells.

The results suggest that a mutation of SCD1, but not LEPR or ABCG2, might be useful as a DNA marker to decrease reproductive problems and improve production traits in ian Holstein dairy cows.
Title: Effect of LEPR, ABCG2 and SCD1 gene polymorphisms on reproductive traits in the ian Holstein cattle.

The following SCD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43666	NM_173959.4 Latest version!	Bos taurus stearoyl-CoA desaturase (SCD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCD ( NM_173959.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776384.3 Bos taurus stearoyl-CoA desaturase (SCD), mRNA.

Price & Availability↑

CloneID	OBa43666
Clone ID Related Accession (Same CDS sequence)	NM_173959.4
Accession Version	NM_173959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	acyl-CoA desaturase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY241933.1. On or before Feb 14, 2012 this sequence version replaced XM_002698490.1, NM_173959.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY241933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGCCC ACTTGCTGCA AGAGGAGATC TCTAGCTCCT ACACAACCAC CACCACCATC 
ACAGCACCTC CTTCCAGGGT CCTGCAGAAT GGAGGGGGCA AATTGGAGAA GACTCCCCTA 
TACTTGGAAG AAGACATCCG CCCTGAAATG AGAGATGACA TCTATGACCC AACTTACCAG 
GATAAGGAGG GCCCAAAGCC CAAGCTTGAG TATGTTTGGA GAAACATCAT CCTCATGTCT 
CTGTTACACT TGGGAGCCCT ATATGGGATC ACATTGATCC CCACCTGCAA GATATACACC 
TATATCTGGG TGTTATTCTA CTATCTGATG GGTGCCCTGG GCATCACAGC AGGGGCCCAT 
CGCCTGTGGA GTCACCGAAC CTACAAAGCT CGGCTGCCTC TGCGGGTCTT CCTGATCATT 
GGCAACACCA TGGCGTTCCA GAATGACGTT TTTGAATGGT CCCGAGATCA CCGTGCCCAC 
CACAAGTTTT CAGAAACGGA TGCCGACCCC CACAATTCCC GACGTGGCTT TTTCTTCTCT 
CACGTGGGTT GGCTGCTTGT GCGCAAACAC CCAGCTGTCA AAGAAAAGGG TTCCACGCTA 
AATTTATCCG ACCTAAGAGC CGAGAAGCTG GTGATGTTCC AGAGGAGGTA CTACAAACCT 
GGTGTCCTGT TGTTGTGCTT CATCCTGCCC ACACTCGTGC CATGGTATCT GTGGGATGAA 
ACGTTTCAAA ACAGCCTGTT TTTTGCCACC TTATTCCGTT ATGCCCTTGG GCTCAACGTC 
ACCTGGCTGG TGAATAGTGC TGCCCATATG TATGGATACC GCCCTTATGA CAAGACCATC 
AACCCCCGAG AGAATATTCT GGTTTCCCTG GGAGCTGCGG GTGAGGGCTT CCACAACTAC 
CACCACACCT TTCCTTATGA CTACTCAGCC AGTGAGTACC GCTGGCACAT CAACTTTACC 
ACGTTCTTCA TTGATTGCAT GGCTGCCATC GGTCTGGCTT ATGACCGGAA GAAAGTATCC 
AAGGCTGCCA TCTTGGCCAG GATAAAAAGA ACTGGAGAGG AAAGCTACAA GAGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776384.3
CDS	145..1224
Translation	MPAHLLQEEISSSYTTTTTITAPPSRVLQNGGGKLEKTPLYLEEDIRPEMRDDIYDPTYQDKEGPKPKLEYVWRNIILMSLLHLGALYGITLIPTCKIYTYIWVLFYYLMGALGITAGAHRLWSHRTYKARLPLRVFLIIGNTMAFQNDVFEWSRDHRAHHKFSETDADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLNLSDLRAEKLVMFQRRYYKPGVLLLCFILPTLVPWYLWDETFQNSLFFATLFRYALGLNVTWLVNSAAHMYGYRPYDKTINPRENILVSLGAAGEGFHNYHHTFPYDYSASEYRWHINFTTFFIDCMAAIGLAYDRKKVSKAAILARIKRTGEESYKSG

Target ORF information:

RefSeq Version	NM_173959.4
Organism	Bos taurus(cattle)
Definition	Bos taurus stearoyl-CoA desaturase (SCD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173959.4

ORF Insert Sequence:

ATGCCGGCCC ACTTGCTGCA AGAGGAGATC TCTAGCTCCT ACACAACCAC CACCACCATC 
ACAGCACCTC CTTCCAGGGT CCTGCAGAAT GGAGGGGGCA AATTGGAGAA GACTCCCCTA 
TACTTGGAAG AAGACATCCG CCCTGAAATG AGAGATGACA TCTATGACCC AACTTACCAG 
GATAAGGAGG GCCCAAAGCC CAAGCTTGAG TATGTTTGGA GAAACATCAT CCTCATGTCT 
CTGTTACACT TGGGAGCCCT ATATGGGATC ACATTGATCC CCACCTGCAA GATATACACC 
TATATCTGGG TGTTATTCTA CTATCTGATG GGTGCCCTGG GCATCACAGC AGGGGCCCAT 
CGCCTGTGGA GTCACCGAAC CTACAAAGCT CGGCTGCCTC TGCGGGTCTT CCTGATCATT 
GGCAACACCA TGGCGTTCCA GAATGACGTT TTTGAATGGT CCCGAGATCA CCGTGCCCAC 
CACAAGTTTT CAGAAACGGA TGCCGACCCC CACAATTCCC GACGTGGCTT TTTCTTCTCT 
CACGTGGGTT GGCTGCTTGT GCGCAAACAC CCAGCTGTCA AAGAAAAGGG TTCCACGCTA 
AATTTATCCG ACCTAAGAGC CGAGAAGCTG GTGATGTTCC AGAGGAGGTA CTACAAACCT 
GGTGTCCTGT TGTTGTGCTT CATCCTGCCC ACACTCGTGC CATGGTATCT GTGGGATGAA 
ACGTTTCAAA ACAGCCTGTT TTTTGCCACC TTATTCCGTT ATGCCCTTGG GCTCAACGTC 
ACCTGGCTGG TGAATAGTGC TGCCCATATG TATGGATACC GCCCTTATGA CAAGACCATC 
AACCCCCGAG AGAATATTCT GGTTTCCCTG GGAGCTGCGG GTGAGGGCTT CCACAACTAC 
CACCACACCT TTCCTTATGA CTACTCAGCC AGTGAGTACC GCTGGCACAT CAACTTTACC 
ACGTTCTTCA TTGATTGCAT GGCTGCCATC GGTCTGGCTT ATGACCGGAA GAAAGTATCC 
AAGGCTGCCA TCTTGGCCAG GATAAAAAGA ACTGGAGAGG AAAGCTACAA GAGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic mechanisms contribute to decrease stearoyl-CoA desaturase 1 expression in the liver of dairy cows after prolonged feeding of high-concentrate diet.
Journal of dairy science(2017 Dec)
Xu TL,Seyfert HM,Shen XZ

Epistatic effects between pairs of the growth hormone secretagogue receptor 1a, growth hormone, growth hormone receptor, non-SMC condensin I complex, subunit G and stearoyl-CoA desaturase genes on carcass, price-related and fatty acid composition traits in Japanese Black cattle.
Animal science journal = Nihon chikusan Gakkaiho(2017 Nov)
Komatsu M,Nishino K,Fujimori Y,Haga Y,Iwama N,Arakawa A,Aihara Y,Takeda H,Takahashi H

A post-GWAS confirming the SCD gene associated with milk medium- and long-chain unsaturated fatty acids in Chinese Holstein population.
Animal genetics47(4)483-90(2016 Aug)
Li C,Sun D,Zhang S,Liu L,Alim MA,Zhang Q

Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.
BMC molecular biology17(1)16(2016 07)
Xu T,Shen X,Seyfert HM

Milk fatty acid profile is modulated by DGAT1 and SCD1 genotypes in dairy cattle on pasture and strategic supplementation.
Genetics and molecular research : GMR15(2)(2016 May)
Carvajal AM,Huircan P,Dezamour JM,Subiabre I,Kerr B,Morales R,Ungerfeld EM

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SCD cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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