Scd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scd
Entrez Gene ID	246074
Full Name	stearoyl-CoA desaturase
Synonyms	Scd1
General protein information	Preferred Names stearoyl-CoA desaturase Names acyl-CoA desaturase 1 delta(9)-desaturase 1 fatty acid desaturase 1 stearoyl-CoA desaturase 1 stearoyl-Coenzyme A desaturase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54
Summary	enzyme involved in the synthesis and regulation of unsaturated fatty acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231433.2	XP_006231495.1	acyl-CoA desaturase 1 isoform X1
NM_139192.2	NP_631931.2	acyl-CoA desaturase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	desat2	NP_650201.1
Gallus gallus (chicken)	SCD	NP_990221.1
Rattus norvegicus (Norway rat)	Scd1	NP_631931.2
Bos taurus (cattle)	SCD	NP_776384.3
Mus musculus (house mouse)	Scd1	NP_033153.2
Danio rerio (zebrafish)	scdb	NP_001018541.1
Xenopus tropicalis (tropical clawed frog)	scd	NP_001027500.1
Homo sapiens (human)	SCD	NP_005054.3
Pan troglodytes (chimpanzee)	SCD	XP_001168563.1
	SCD	XP_001107910.2
Canis lupus familiaris (dog)	SCD	XP_543968.2

Gene Ontology
Bibliography

Related articles in PubMed

miR-192-5p regulates lipid synthesis in non-alcoholic fatty liver disease through SCD-1.
Liu XL, Cao HX, Wang BC, Xin FZ, Zhang RN, Zhou D, Yang RX, Zhao ZH, Pan Q, Fan JG
World journal of gastroenterology23(46)8140-8151(2017 Dec)

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

Long-term fructose consumption prolongs hepatic stearoyl-CoA desaturase 1 activity independent of upstream regulation in rats.
Liu L, Wang S, Yao L, Li JX, Ma P, Jiang LR, Ke DZ, Pan YQ, Wang JW
Biochemical and biophysical research communications479(4)643-648(2016 Oct)

Inhibition of SCD1 impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic ?-cells.
Janikiewicz J, Hanzelka K, Dziewulska A, Kozinski K, Dobrzyn P, Bernas T, Dobrzyn A
Journal of lipid research56(10)1901-11(2015 Oct)

X-ray structure of a mammalian stearoyl-CoA desaturase.
Bai Y, McCoy JG, Levin EJ, Sobrado P, Rajashankar KR, Fox BG, Zhou M
Nature524(7564)252-6(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SCD1 overexpression in fatty liver disease is not affected by fasting after long-term fructose consumption in rats. Regulation of SCD1 plays an important role in fructose-induced hepatic steatosis.
Title: Long-term fructose consumption prolongs hepatic stearoyl-CoA desaturase 1 activity independent of upstream regulation in rats.

Authors investigated whether SCD1 activity is engaged in palmitate-induced pancreatic beta-cell autophagy
Title: Inhibition of SCD1 impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic ?-cells.

SCD1 activity and SCD1 gene expression were considerably up-regulated in Goto-Kakizaki rats, and these levels were largely comparable to those in obese Zucker (fa/fa) rats.
Title: Up-regulation of stearoyl-CoA desaturase 1 increases liver MUFA content in obese Zucker but not Goto-Kakizaki rats.

Adipose tissue stearoyl-CoA desaturase 1 index is increased in obesity-prone rats fed a high-fat diet.
Title: Adipose tissue stearoyl-CoA desaturase 1 index is increased and linoleic acid is decreased in obesity-prone rats fed a high-fat diet.

Data suggest that down-regulation of stearoyl-CoA desaturase Scd1 is a mechanism by which n-3 fatty acids repress constitutive triglyceride biosynthesis.
Title: Omega-3 fatty acid deficiency increases stearoyl-CoA desaturase expression and activity indices in rat liver: positive association with non-fasting plasma triglyceride levels.

The following Scd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02261	NM_139192.2 Latest version!	Rattus norvegicus stearoyl-CoA desaturase (Scd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa02261	XM_006231433.2 Latest version!	Rattus norvegicus stearoyl-CoA desaturase (Scd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scd ( NM_139192.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_631931.2 Rattus norvegicus stearoyl-CoA desaturase (Scd), mRNA.

Price & Availability↑

CloneID	ORa02261
Clone ID Related Accession (Same CDS sequence)	XM_006231433.2 , NM_139192.2
Accession Version	NM_139192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	acyl-CoA desaturase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF509569.1. On Jun 10, 2007 this sequence version replaced NM_139192.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF509568.1, AF509569.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCCC ACATGCTCCA AGAGATCTCC AGTTCCTACA CGACCACCAC TACCATCACA 
GAGCCTCCTT CCGGAAACCT GCAGAATGGA CGAGAGAAGA TGAAGAAGGT GCCCCTCTAT 
CTGGAAGAAG ACATCCGTCC TGAAATGAGA GAAGATATCC ACGACCCCAG CTACCAGGAT 
GAGGAGGGGC CCCCGCCCAA GCTGGAGTAC GTCTGGAGGA ACATCATCCT CATGGCCCTG 
CTGCACGTGG GAGCCCTGTA CGGGATCACA CTGATCCCCT CCTCCAAGGT CTACACCTTG 
CTCTGGGGGA TATTTTACTA CCTTATCAGT GCCCTGGGCA TCACAGCCGG GGCTCATCGC 
CTGTGGAGCC ACAGGACTTA CAAGGCTCGG CTGCCCCTGA GGATCTTCCT CATCATTGCC 
AACACCATGG CGTTCCAGAA CGATGTGTAT GAATGGGCCC GGGATCACCG CGCCCACCAC 
AAGTTCTCTG AGACACACGC CGACCCTCAC AACTCCCGCC GTGGCTTTTT CTTCTCTCAC 
GTGGGTTGGC TGCTTGTGCG CAAACACCCG GCTGTCAAAG AGAAGGGCGG AAAGCTGGAC 
ATGTCTGACC TGAAAGCTGA GAAGCTGGTG ATGTTCCAGA GGAGGTACTA CAAGCCTGGC 
CTCCTGCTCA TGTGCTTCAT CCTGCCCACA CTGGTGCCCT GGTACTGCTG GGGCGAAACT 
TTCCTGCACA GCCTGTTCGT CAGCACCTTC TTGAGATACA CTCTGGTGCT CAACGCCACC 
TGGCTGGTGA ACAGCGCTGC CCACCTCTAT GGGTATCGCC CCTACGACAA GAACATTCAA 
TCTCGGGAGA ACATCCTGGT TTCCCTGGGT GCCGTGGGCG AGGGCTTCCA CAACTACCAT 
CACGCCTTCC CCTACGACTA CTCTGCCAGT GAGTACCGCT GGCACATCAA CTTCACCACG 
TTCTTCATCG ACTGCATGGC TGCCCTGGGC CTGGCTTACG ACCGGAAAAA AGTTTCTAAG 
GCCGCTGTCT TAGCCAGGAT TAAGAGAACT GGAGATGGGA GCCACAAGAG TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_631931.2
CDS	49..1125
Translation	MPAHMLQEISSSYTTTTTITEPPSGNLQNGREKMKKVPLYLEEDIRPEMREDIHDPSYQDEEGPPPKLEYVWRNIILMALLHVGALYGITLIPSSKVYTLLWGIFYYLISALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFLHSLFVSTFLRYTLVLNATWLVNSAAHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHAFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAVLARIKRTGDGSHKSS

Target ORF information:

RefSeq Version	NM_139192.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus stearoyl-CoA desaturase (Scd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139192.2

ORF Insert Sequence:

ATGCCGGCCC ACATGCTCCA AGAGATCTCC AGTTCCTACA CGACCACCAC TACCATCACA 
GAGCCTCCTT CCGGAAACCT GCAGAATGGA CGAGAGAAGA TGAAGAAGGT GCCCCTCTAT 
CTGGAAGAAG ACATCCGTCC TGAAATGAGA GAAGATATCC ACGACCCCAG CTACCAGGAT 
GAGGAGGGGC CCCCGCCCAA GCTGGAGTAC GTCTGGAGGA ACATCATCCT CATGGCCCTG 
CTGCACGTGG GAGCCCTGTA CGGGATCACA CTGATCCCCT CCTCCAAGGT CTACACCTTG 
CTCTGGGGGA TATTTTACTA CCTTATCAGT GCCCTGGGCA TCACAGCCGG GGCTCATCGC 
CTGTGGAGCC ACAGGACTTA CAAGGCTCGG CTGCCCCTGA GGATCTTCCT CATCATTGCC 
AACACCATGG CGTTCCAGAA CGATGTGTAT GAATGGGCCC GGGATCACCG CGCCCACCAC 
AAGTTCTCTG AGACACACGC CGACCCTCAC AACTCCCGCC GTGGCTTTTT CTTCTCTCAC 
GTGGGTTGGC TGCTTGTGCG CAAACACCCG GCTGTCAAAG AGAAGGGCGG AAAGCTGGAC 
ATGTCTGACC TGAAAGCTGA GAAGCTGGTG ATGTTCCAGA GGAGGTACTA CAAGCCTGGC 
CTCCTGCTCA TGTGCTTCAT CCTGCCCACA CTGGTGCCCT GGTACTGCTG GGGCGAAACT 
TTCCTGCACA GCCTGTTCGT CAGCACCTTC TTGAGATACA CTCTGGTGCT CAACGCCACC 
TGGCTGGTGA ACAGCGCTGC CCACCTCTAT GGGTATCGCC CCTACGACAA GAACATTCAA 
TCTCGGGAGA ACATCCTGGT TTCCCTGGGT GCCGTGGGCG AGGGCTTCCA CAACTACCAT 
CACGCCTTCC CCTACGACTA CTCTGCCAGT GAGTACCGCT GGCACATCAA CTTCACCACG 
TTCTTCATCG ACTGCATGGC TGCCCTGGGC CTGGCTTACG ACCGGAAAAA AGTTTCTAAG 
GCCGCTGTCT TAGCCAGGAT TAAGAGAACT GGAGATGGGA GCCACAAGAG TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scd ( XM_006231433.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231495.1 Rattus norvegicus stearoyl-CoA desaturase (Scd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02261
Clone ID Related Accession (Same CDS sequence)	XM_006231433.2 , NM_139192.2
Accession Version	XM_006231433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acyl-CoA desaturase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCC ACATGCTCCA AGAGATCTCC AGTTCCTACA CGACCACCAC TACCATCACA 
GAGCCTCCTT CCGGAAACCT GCAGAATGGA CGAGAGAAGA TGAAGAAGGT GCCCCTCTAT 
CTGGAAGAAG ACATCCGTCC TGAAATGAGA GAAGATATCC ACGACCCCAG CTACCAGGAT 
GAGGAGGGGC CCCCGCCCAA GCTGGAGTAC GTCTGGAGGA ACATCATCCT CATGGCCCTG 
CTGCACGTGG GAGCCCTGTA CGGGATCACA CTGATCCCCT CCTCCAAGGT CTACACCTTG 
CTCTGGGGGA TATTTTACTA CCTTATCAGT GCCCTGGGCA TCACAGCCGG GGCTCATCGC 
CTGTGGAGCC ACAGGACTTA CAAGGCTCGG CTGCCCCTGA GGATCTTCCT CATCATTGCC 
AACACCATGG CGTTCCAGAA CGATGTGTAT GAATGGGCCC GGGATCACCG CGCCCACCAC 
AAGTTCTCTG AGACACACGC CGACCCTCAC AACTCCCGCC GTGGCTTTTT CTTCTCTCAC 
GTGGGTTGGC TGCTTGTGCG CAAACACCCG GCTGTCAAAG AGAAGGGCGG AAAGCTGGAC 
ATGTCTGACC TGAAAGCTGA GAAGCTGGTG ATGTTCCAGA GGAGGTACTA CAAGCCTGGC 
CTCCTGCTCA TGTGCTTCAT CCTGCCCACA CTGGTGCCCT GGTACTGCTG GGGCGAAACT 
TTCCTGCACA GCCTGTTCGT CAGCACCTTC TTGAGATACA CTCTGGTGCT CAACGCCACC 
TGGCTGGTGA ACAGCGCTGC CCACCTCTAT GGGTATCGCC CCTACGACAA GAACATTCAA 
TCTCGGGAGA ACATCCTGGT TTCCCTGGGT GCCGTGGGCG AGGGCTTCCA CAACTACCAT 
CACGCCTTCC CCTACGACTA CTCTGCCAGT GAGTACCGCT GGCACATCAA CTTCACCACG 
TTCTTCATCG ACTGCATGGC TGCCCTGGGC CTGGCTTACG ACCGGAAAAA AGTTTCTAAG 
GCCGCTGTCT TAGCCAGGAT TAAGAGAACT GGAGATGGGA GCCACAAGAG TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231495.1
CDS	184..1260
Translation	MPAHMLQEISSSYTTTTTITEPPSGNLQNGREKMKKVPLYLEEDIRPEMREDIHDPSYQDEEGPPPKLEYVWRNIILMALLHVGALYGITLIPSSKVYTLLWGIFYYLISALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFLHSLFVSTFLRYTLVLNATWLVNSAAHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHAFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAVLARIKRTGDGSHKSS

Target ORF information:

RefSeq Version	XM_006231433.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus stearoyl-CoA desaturase (Scd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231433.2

ORF Insert Sequence:

ATGCCGGCCC ACATGCTCCA AGAGATCTCC AGTTCCTACA CGACCACCAC TACCATCACA 
GAGCCTCCTT CCGGAAACCT GCAGAATGGA CGAGAGAAGA TGAAGAAGGT GCCCCTCTAT 
CTGGAAGAAG ACATCCGTCC TGAAATGAGA GAAGATATCC ACGACCCCAG CTACCAGGAT 
GAGGAGGGGC CCCCGCCCAA GCTGGAGTAC GTCTGGAGGA ACATCATCCT CATGGCCCTG 
CTGCACGTGG GAGCCCTGTA CGGGATCACA CTGATCCCCT CCTCCAAGGT CTACACCTTG 
CTCTGGGGGA TATTTTACTA CCTTATCAGT GCCCTGGGCA TCACAGCCGG GGCTCATCGC 
CTGTGGAGCC ACAGGACTTA CAAGGCTCGG CTGCCCCTGA GGATCTTCCT CATCATTGCC 
AACACCATGG CGTTCCAGAA CGATGTGTAT GAATGGGCCC GGGATCACCG CGCCCACCAC 
AAGTTCTCTG AGACACACGC CGACCCTCAC AACTCCCGCC GTGGCTTTTT CTTCTCTCAC 
GTGGGTTGGC TGCTTGTGCG CAAACACCCG GCTGTCAAAG AGAAGGGCGG AAAGCTGGAC 
ATGTCTGACC TGAAAGCTGA GAAGCTGGTG ATGTTCCAGA GGAGGTACTA CAAGCCTGGC 
CTCCTGCTCA TGTGCTTCAT CCTGCCCACA CTGGTGCCCT GGTACTGCTG GGGCGAAACT 
TTCCTGCACA GCCTGTTCGT CAGCACCTTC TTGAGATACA CTCTGGTGCT CAACGCCACC 
TGGCTGGTGA ACAGCGCTGC CCACCTCTAT GGGTATCGCC CCTACGACAA GAACATTCAA 
TCTCGGGAGA ACATCCTGGT TTCCCTGGGT GCCGTGGGCG AGGGCTTCCA CAACTACCAT 
CACGCCTTCC CCTACGACTA CTCTGCCAGT GAGTACCGCT GGCACATCAA CTTCACCACG 
TTCTTCATCG ACTGCATGGC TGCCCTGGGC CTGGCTTACG ACCGGAAAAA AGTTTCTAAG 
GCCGCTGTCT TAGCCAGGAT TAAGAGAACT GGAGATGGGA GCCACAAGAG TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-192-5p regulates lipid synthesis in non-alcoholic fatty liver disease through SCD-1.
World journal of gastroenterology23(46)8140-8151(2017 Dec)
Liu XL,Cao HX,Wang BC,Xin FZ,Zhang RN,Zhou D,Yang RX,Zhao ZH,Pan Q,Fan JG

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

Long-term fructose consumption prolongs hepatic stearoyl-CoA desaturase 1 activity independent of upstream regulation in rats.
Biochemical and biophysical research communications479(4)643-648(2016 Oct)
Liu L,Wang S,Yao L,Li JX,Ma P,Jiang LR,Ke DZ,Pan YQ,Wang JW

Inhibition of SCD1 impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic ?-cells.
Journal of lipid research56(10)1901-11(2015 Oct)
Janikiewicz J,Hanzelka K,Dziewulska A,Kozinski K,Dobrzyn P,Bernas T,Dobrzyn A

X-ray structure of a mammalian stearoyl-CoA desaturase.
Nature524(7564)252-6(2015 Aug)
Bai Y,McCoy JG,Levin EJ,Sobrado P,Rajashankar KR,Fox BG,Zhou M

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Scd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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