Desat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Desat2
Entrez Gene ID	41536
Full Name	Desaturase 2
Synonyms	7-11HD,CG5925,Dmel\CG5925,Fad,desat,desat-2,desat2,desat2/Fad,ds2
General protein information	Preferred Names Desaturase 2 Names CG5925 gene product from transcript CG5925-RA 7,11-heptacosadiene modifier CG5925-PA Desat2-PA Desaturase2 desat2 desaturase desaturase 2 desaturase-2 fatty acid desaturase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87B10-87B10\|3-51.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_141944.3	NP_650201.1	desaturase 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	desat2	NP_650201.1
Gallus gallus (chicken)	SCD	NP_990221.1
Rattus norvegicus (Norway rat)	Scd1	NP_631931.2
Bos taurus (cattle)	SCD	NP_776384.3
Mus musculus (house mouse)	Scd1	NP_033153.2
Danio rerio (zebrafish)	scdb	NP_001018541.1
Xenopus tropicalis (tropical clawed frog)	scd	NP_001027500.1
Homo sapiens (human)	SCD	NP_005054.3
Pan troglodytes (chimpanzee)	SCD	XP_001168563.1
	SCD	XP_001107910.2
Canis lupus familiaris (dog)	SCD	XP_543968.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The allele with an additional deletion in exon 1 seems to play some role in mating success, which is associated with shorter courtship latency and courtship duration
Title: Deletions in the desaturase2 gene in Israeli populations of Drosophila melanogaster: possible role in mate recognition.

Geographical variation at the pheromone locus desaturase-2, and morphology and courtship behavior across the US-Caribbean region.
Title: African morphology, behavior and phermones underlie incipient sexual isolation between us and Caribbean Drosophila melanogaster.

This study failed replicate earlier results showing that carriers of M alleles are uniformly more cold resistant and less starvation resistant than carriers of Z alleles; it did demonstrate some evidence for sexual isolation due to desat2.
Title: Does the desaturase-2 locus in Drosophila melanogaster cause adaptation and sexual isolation?

an allele of ds2 from the Zimbabwe population was introduced into Cosmopolitan flies; results show the Cosmopolitan allele confers resistance to cold as well as susceptibility to starvation when the entire genetic background is otherwise identical
Title: Ecological adaptation during incipient speciation revealed by precise gene replacement.

The following Desat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Desat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04691	NM_141944.3 Latest version!	Drosophila melanogaster desaturase 2 (Desat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Desat2 ( NM_141944.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650201.1 Drosophila melanogaster desaturase 2 (Desat2), mRNA.

Price & Availability↑

CloneID	OFa04691
Clone ID Related Accession (Same CDS sequence)	NM_141944.3
Accession Version	NM_141944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	desaturase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141944.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCCCT ACTCAAGGAT ATACCATCAG GATAAGTCCT CCAGAGAAAC GGGTGTCCTT 
TTCGAGGACG ATGCGCAAAC AGTCGACGGT GACCTGACCA CCGACAGATT CCAGTTGAAA 
CGAGCTGAGA AGCGTCGGCT ACCCCTAGTC TGGCGGAATA TAATCCTGTT CGCATTGGTC 
CACCTGGCTG CTCTATATGG CCTGCACTCC ATATTCACAA GGGCGAAACT GGCAACCACA 
CTTTTCGCTG CCGGATTGTA TATCATTGGC ATGCTGGGCG TTACAGCCGG TGCCCATCGT 
CTATGGGCGC ATCGCACCTA CAAGGCCAAG TGGCCCCTGC GCCTGCTCCT GGTCATCTTC 
AATACGATCG CCTTTCAGGA TGCCGTCTAC CATTGGGCCC GTGACCATCG CGTCCACCAC 
AAATACTCGG AAACTGATGC AGATCCTCAC AACGCCACCA GGGGATTTTT CTTCTCGCAT 
GTGGGCTGGC TGCTCTGCAA AAAGCATCCG GATATTAAGG AAAAGGGAAG GGGACTGGAT 
CTCTCCGACC TGCGCGCCGA TCCCATCTTG ATGTTCCAAC GAAAGCACTA TTATATCCTC 
ATGCCGCTGG CCTGCTTCGT TCTGCCCACC GTTATTCCAA TGGTTTACTG GAATGAGACG 
CTGGCCTCCT CTTGGTTTGT GGCCACCATG TTCAGGTGGT GCTTCCAGCT AAACATGACC 
TGGCTGGTGA ACAGCGCGGC CCATAAATTC GGAAATCGCC CATACGACAA GACCATGAAT 
CCTACCCAGA ATGCCTTTGT ATCGGCCTTC ACCTTTGGCG AGGGGTGGCA CAACTACCAT 
CACGCGTTTC CGTGGGACTA CAAGACGGCC GAATGGGGTT GCTACTCCCT GAATATAACG 
ACGGCCTTCA TCGACTTGTT CGCCAAGATC GGCTGGGCCT ACGACCTGAA GACGGTGGCT 
CCCGACGTGA TCCAGCGACG AGTGCTCCGC ACTGGCGACG GTAGCCACGA ACTGTGGGGC 
TGGGGCGATA AGGACCTGAC AGCGGAGGAT GCCAGGAACG TGCTCCTTGT GGATAAAAGT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650201.1
CDS	174..1259
Translation	MAPYSRIYHQDKSSRETGVLFEDDAQTVDGDLTTDRFQLKRAEKRRLPLVWRNIILFALVHLAALYGLHSIFTRAKLATTLFAAGLYIIGMLGVTAGAHRLWAHRTYKAKWPLRLLLVIFNTIAFQDAVYHWARDHRVHHKYSETDADPHNATRGFFFSHVGWLLCKKHPDIKEKGRGLDLSDLRADPILMFQRKHYYILMPLACFVLPTVIPMVYWNETLASSWFVATMFRWCFQLNMTWLVNSAAHKFGNRPYDKTMNPTQNAFVSAFTFGEGWHNYHHAFPWDYKTAEWGCYSLNITTAFIDLFAKIGWAYDLKTVAPDVIQRRVLRTGDGSHELWGWGDKDLTAEDARNVLLVDKSR

Target ORF information:

RefSeq Version	NM_141944.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster desaturase 2 (Desat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141944.3

ORF Insert Sequence:

ATGGCGCCCT ACTCAAGGAT ATACCATCAG GATAAGTCCT CCAGAGAAAC GGGTGTCCTT 
TTCGAGGACG ATGCGCAAAC AGTCGACGGT GACCTGACCA CCGACAGATT CCAGTTGAAA 
CGAGCTGAGA AGCGTCGGCT ACCCCTAGTC TGGCGGAATA TAATCCTGTT CGCATTGGTC 
CACCTGGCTG CTCTATATGG CCTGCACTCC ATATTCACAA GGGCGAAACT GGCAACCACA 
CTTTTCGCTG CCGGATTGTA TATCATTGGC ATGCTGGGCG TTACAGCCGG TGCCCATCGT 
CTATGGGCGC ATCGCACCTA CAAGGCCAAG TGGCCCCTGC GCCTGCTCCT GGTCATCTTC 
AATACGATCG CCTTTCAGGA TGCCGTCTAC CATTGGGCCC GTGACCATCG CGTCCACCAC 
AAATACTCGG AAACTGATGC AGATCCTCAC AACGCCACCA GGGGATTTTT CTTCTCGCAT 
GTGGGCTGGC TGCTCTGCAA AAAGCATCCG GATATTAAGG AAAAGGGAAG GGGACTGGAT 
CTCTCCGACC TGCGCGCCGA TCCCATCTTG ATGTTCCAAC GAAAGCACTA TTATATCCTC 
ATGCCGCTGG CCTGCTTCGT TCTGCCCACC GTTATTCCAA TGGTTTACTG GAATGAGACG 
CTGGCCTCCT CTTGGTTTGT GGCCACCATG TTCAGGTGGT GCTTCCAGCT AAACATGACC 
TGGCTGGTGA ACAGCGCGGC CCATAAATTC GGAAATCGCC CATACGACAA GACCATGAAT 
CCTACCCAGA ATGCCTTTGT ATCGGCCTTC ACCTTTGGCG AGGGGTGGCA CAACTACCAT 
CACGCGTTTC CGTGGGACTA CAAGACGGCC GAATGGGGTT GCTACTCCCT GAATATAACG 
ACGGCCTTCA TCGACTTGTT CGCCAAGATC GGCTGGGCCT ACGACCTGAA GACGGTGGCT 
CCCGACGTGA TCCAGCGACG AGTGCTCCGC ACTGGCGACG GTAGCCACGA ACTGTGGGGC 
TGGGGCGATA AGGACCTGAC AGCGGAGGAT GCCAGGAACG TGCTCCTTGT GGATAAAAGT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Desat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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