USP54 cDNA ORF clone, Bos taurus(cattle)

The following USP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa12475 XM_015461045.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_024986825.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_024986826.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_005226504.4
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_005226501.4
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_005226500.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_024986824.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12475 XM_005226503.4
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa12476 XM_024986827.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa12477 XM_024986829.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa12477 XM_024986830.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa231708 XM_024986828.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231709 XM_024986832.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231710 XM_024986833.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa59795 XM_024986831.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa34295 XM_005226504.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005226503.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005226501.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_015469846.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005199534.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005199533.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_002705400.5
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_003587990.4
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_015461045.1
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005199536.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005199537.3
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34295 XM_005226500.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa34296 XM_005226505.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa34296 XM_005199538.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa34297 XM_005226506.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa34297 XM_005199539.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa59797 XM_010820659.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa59797 XM_010799131.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa59798 XM_010820660.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa59798 XM_010799132.2
Latest version!
Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_005226503.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226503.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226560.2
    CDS883..5952
    Translation

    Target ORF information:

    RefSeq Version XM_005226503.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226503.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_005226501.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226558.2
    CDS892..5961
    Translation

    Target ORF information:

    RefSeq Version XM_005226501.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226501.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_005226504.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226561.2
    CDS857..5926
    Translation

    Target ORF information:

    RefSeq Version XM_005226504.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226504.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_024986826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842594.1
    CDS730..5799
    Translation

    Target ORF information:

    RefSeq Version XM_024986826.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_024986825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842593.1
    CDS772..5841
    Translation

    Target ORF information:

    RefSeq Version XM_024986825.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_015461045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316531.2
    CDS785..5854
    Translation

    Target ORF information:

    RefSeq Version XM_015461045.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_005226500.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226557.2
    CDS926..5995
    Translation

    Target ORF information:

    RefSeq Version XM_005226500.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226500.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12475
    Clone ID Related Accession (Same CDS sequence) XM_005226503.4 , XM_005226501.4 , XM_005226504.4 , XM_024986826.1 , XM_024986825.1 , XM_015461045.2 , XM_005226500.3 , XM_024986824.1
    Accession Version XM_024986824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842592.1
    CDS985..6054
    Translation

    Target ORF information:

    RefSeq Version XM_024986824.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12476
    Clone ID Related Accession (Same CDS sequence) XM_024986827.1
    Accession Version XM_024986827.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842595.1
    CDS883..5832
    Translation

    Target ORF information:

    RefSeq Version XM_024986827.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCAG TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12477
    Clone ID Related Accession (Same CDS sequence) XM_024986829.1 , XM_024986830.1
    Accession Version XM_024986829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842597.1
    CDS882..5780
    Translation

    Target ORF information:

    RefSeq Version XM_024986829.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12477
    Clone ID Related Accession (Same CDS sequence) XM_024986829.1 , XM_024986830.1
    Accession Version XM_024986830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842598.1
    CDS890..5788
    Translation

    Target ORF information:

    RefSeq Version XM_024986830.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231708
    Clone ID Related Accession (Same CDS sequence) XM_024986828.1
    Accession Version XM_024986828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG
    CAAGAGGCAG ATTCAGTGTT TGAAGAGTCG CTGCATCTGG AGCAGAAGGG AGACTGTGCT
    GCCGCTTTGG CTCTCTGTAA CGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC AGTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842596.1
    CDS883..5784
    Translation

    Target ORF information:

    RefSeq Version XM_024986828.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG
    CAAGAGGCAG ATTCAGTGTT TGAAGAGTCG CTGCATCTGG AGCAGAAGGG AGACTGTGCT
    GCCGCTTTGG CTCTCTGTAA CGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC AGTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231709
    Clone ID Related Accession (Same CDS sequence) XM_024986832.1
    Accession Version XM_024986832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842600.1
    CDS882..5660
    Translation

    Target ORF information:

    RefSeq Version XM_024986832.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCAGT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231710
    Clone ID Related Accession (Same CDS sequence) XM_024986833.1
    Accession Version XM_024986833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGAGAAA CACTGAAAGA GAAGCAGGCT CCTAGGAATG CCTCCAAATC ATCCAGCAGC
    ACCAACAGGC TAAGGGATTT TAAAGAGACA GTCAGCAATA TGATCCATAA CAGACCATCC
    CTGGCTTCTC AGACCACCCT AGGACCTCCT TGTGTAGGGA AGGGAGGAGA ACAGACGGAC
    AAAAAACCTC CTCGGAGCCT GCCTTTACAC TCTCGTGACT GGGAAGTAGA GAGTACCAGC
    AGCGAGTCAA AATCCAGTTC TTCTAGCAAA TATCGTCCAA CATGGAGACC CAAACGGGAG
    TCTCTGAATA TTGACAGTAT CTTTAGTAAG GACAAAAGGA AGCACAGTGG CTATACCCAG
    CTTAGCCCCT TTTCTGAAGA TTCAGCAAAA GAATTTACAC CAGATGAGTC AAGCAAGCCG
    CCTGCTTATG ACATTAGAAC TGGACCAAGC CCCCAGTACA AGATCTGGGG CCCAGCACGG
    CCAGGCTGTC ACTTTTTAGA GCAACACCCT CGCCTCATCC AGCGAATGGA ATCTGGCTAC
    GAGAGCAGTG AGAGGAACAG CAGCAGCCCT GTCAGCCTGG ATGCAGCCCT GCCTGAGAGC
    TCAAGTGTCT GCAGGGATCC AAGTGCTAAG AGGTCAGCAG GATTGGCCCC TTCCTGGCGT
    CACATCCCTA AGTCTCACAG CAGCAGCATC CTGGAGGCAG ACTCTGCCGT GTCCAGGAGT
    GGCTGGATGA AGAACCAGCC CCTTTCAGGT GGGGAGATAT CTTCCAAAAC TGAACTGGAT
    GAATTGCAGG AAGAAGTGGC CAGAAGGGCA CAGGAACAGG AACTTCGAAG GAAACGAGAG
    AAGGAATTAG AGGCAGCTAA AGGGTTTAAC CCTCATCCCA GCCGCTTCAT GGACTTGGAT
    GAACTGCAGA ATCAGGGGAG GAGTGACGGC TTTGAGAGGT CCCTGCAAGA GGCAGATTCA
    GTGTTTGAAG AGTCGCTGCA TCTGGAGCAG AAGGGAGACT GTGCTGCCGC TTTGGCTCTC
    TGTAACGAAG CTATCTCTAA ACTAAGACTT GCCCTGCATG GTGCCAGCTC TAGCACGCAC
    AGCAGAGCCC TAGTCGATAA GAAGTTGCAA ATCAGTATTC GAAAAGCACG GAGCCTGCAG
    GATCGCATGC AGCAGCAGCA ATCATCTCAG CCGCCGTCGC AGCCCTCAAC CTGCCTCCCC
    TCACAGGGAG GAGCCCTTCC TCAGCCAACA AGTGAACAGC CTATCCCGCT CCAAGTATTG
    TTAAGCCAGG AGGCCCAACT GGAACCCTAC ACGAATACGG AGTTTGGGGC CAGTACTTTC
    ATTTACTCAC CTGCTTCCTG CCATGAGCCA CACTCGCCAT TATTCCCAGA GTCATCCGCC
    CTGTCTGCTC CACAGCACAA TTCCCCCAGT GGGTCTGCCT CGAACCTTCT TACTTCTGTT
    GAGGTGGACA GTATTGACCC CTCTGTGTTC CACAGGCAGG GCTTACCTAA AACACCAGGG
    AGACCTGAGA AGAGTTCTCA TCATGGTTGG GAATCAGAGG ATGAGCTGCA AAGCTGCAGG
    GGTGAAAGCA GCAGCTGCGC CAGGTCCCCC CAACGAGAAG GAAGAGACAC TGATCGAGAA
    CAGCTATTCC GAGAAAAGAG GGATCCTGCT GACTCATCCC AGGTGATGCC TTGGCCACAG
    CCAATTGGAA TAATAGCCAC CTCTGAGAGA GGAGAGGCAC ACAGTCTAGG CTGTGGTCCT
    TCAAATTCAG CAGCTCAGCC TAGCCTTTCT CTGTACAGGG CCTGCCACCC CGTAAAACCT
    GCTCCTTCCT CATCTGTGCT TCACTCCTCT AATACTGTAC AGAAAACTAA CCAATGCCTC
    CAAACCCAAA ACTTCAAAAT TCCATTGACT TCAAATATGG ATCGAGGCAG CGAGGAGCCC
    TCTATGCCAG AGTTTCCTAC CACCAAGGGG CTTGTCCGCT CTTTGGCAGA GCAGTTCCAG
    AGGATGCACG GAGCCTCCAC CAGGGATGGT ACAGGTGCCC AGGATAGAAG TTTGCCAAAC
    AGTCTAAGGA AGGACTCTCC ACCTTCCGAC TGTAAGCCTC CTTTCCCACA GAGTCAAGGG
    AAAGGCCACT GGTCTTGGGC AAAACAACAA CCCTCTTTGG ATGGTAGGAA CAAACTGCCT
    TCCTGGGAAG AGCCTGCTAA TCATCCTTCT CTTGCCACGA ACTCTGGGCC GCCTAATGGT
    GAAGCTTCTA GGGGAGGACA GCCCCGGTTG GCAGAGTCAG GTATTCACCA CGGGAAACCG
    TCTCAAGTGA GAGATGCTGG GCCTAAGGAG CTGGGCAGCA GCGTCGACTC GGGCACTTCC
    TTGCCTTCGG ATTCTTGGGT GAATGTCACA AGGCTCTGTG ATTCTCAGCT TAAACATGGG
    GTCCCTGGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACCTGTGT AACCTATCCA
    GAAAGAAACC ACATCATTTT GCATCCACAT TGGAACCAGG ACACAGAGCA GGAGACCTCA
    GAATTGGAGT CTCTCTACCA AGCCAGTCTT CAGGTTTCCC AATCTGGCTG TTCTGGATGT
    GGGCAGCAGG AAGTAGCCTG GAACCCGCTG AGCCAAACAG GCTCTGCAGA TGGTGTAGGG
    AGGAGGTTGC ACTCAGCCCC TGGCCTTGGT CTCTCAAAGA CCCCAACAGC AGAAATGGAG
    CACAGTCTCC ATGAAGCCAA CTCAGTGTCT GCTTCTCAGG CCGCACCTTG CCAAGGCCCC
    AGCCGGGAAT GTGGGGAGGA TGATCAGTAT GGTGCAGAGA ACTTTCGGCG CATCTCACGC
    AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAGGTCC CAGTCTCGTC CCACAGTTTT
    GATTCATCAA GCGTGAGGAA AAAGCCTTTG GAAACCCGGC ATCGTTGTTC CAGCTCCTCT
    TCCCTCCCTG TCATCCATGA CCCTCCTGTA TTTCTCCTTG GTCCCCAACT CTACCCACCC
    CAACCACAGT TCCTGTCCCC AGATGTCTTG ATGCCCAGCA TGGCAGGGGA GCCCCATAGA
    CCCCCAGGAA CTTCGAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT
    TCCGAAGGGG TCAAGTACAC AGGAAAGACT TTGAACTACC GGAGTCTCCC CCATCGTTCC
    AGAACAGATG CCTCCCGGGG ACTGTGGTCA GAGGCCAACC AGCATGTTGG GGCCAGATTC
    CTGACTACTC CAGGGTGCAA GCCTCAGCTA ACCCACACTG CCACGCTACC AGAAAGACAC
    CAGGGCCTTC AGGTTCCTCA TGCTCAGTCC TGGGGGGATC TTTTCCATTC ACCCTCCCAC
    CCTCCTGTTG TTCACCCTGT GTCGCCACCA TCAAGCAGTC TTTGTGTACC CCTGAGGTCA
    GCTTGGAATT CAGGTCCTGT TCCAGGGTTT CGAGCCCCTG GTCCTCGAAG AGTAGATATG
    CCCCCGGATG ATGACTGGAG GCAAAGCAGT TATGCCCTGC CATCTAGGCA CAGGAGGACA
    GGGAGAGAGG AGTTTCTCTT TGTCCTAGCA GATGCCCCTA ACAGAGAACA GATCAGGGCT
    AGAGCCCTGC AGCACAGCAG GTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842601.1
    CDS883..5649
    Translation

    Target ORF information:

    RefSeq Version XM_024986833.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGAGAAA CACTGAAAGA GAAGCAGGCT CCTAGGAATG CCTCCAAATC ATCCAGCAGC
    ACCAACAGGC TAAGGGATTT TAAAGAGACA GTCAGCAATA TGATCCATAA CAGACCATCC
    CTGGCTTCTC AGACCACCCT AGGACCTCCT TGTGTAGGGA AGGGAGGAGA ACAGACGGAC
    AAAAAACCTC CTCGGAGCCT GCCTTTACAC TCTCGTGACT GGGAAGTAGA GAGTACCAGC
    AGCGAGTCAA AATCCAGTTC TTCTAGCAAA TATCGTCCAA CATGGAGACC CAAACGGGAG
    TCTCTGAATA TTGACAGTAT CTTTAGTAAG GACAAAAGGA AGCACAGTGG CTATACCCAG
    CTTAGCCCCT TTTCTGAAGA TTCAGCAAAA GAATTTACAC CAGATGAGTC AAGCAAGCCG
    CCTGCTTATG ACATTAGAAC TGGACCAAGC CCCCAGTACA AGATCTGGGG CCCAGCACGG
    CCAGGCTGTC ACTTTTTAGA GCAACACCCT CGCCTCATCC AGCGAATGGA ATCTGGCTAC
    GAGAGCAGTG AGAGGAACAG CAGCAGCCCT GTCAGCCTGG ATGCAGCCCT GCCTGAGAGC
    TCAAGTGTCT GCAGGGATCC AAGTGCTAAG AGGTCAGCAG GATTGGCCCC TTCCTGGCGT
    CACATCCCTA AGTCTCACAG CAGCAGCATC CTGGAGGCAG ACTCTGCCGT GTCCAGGAGT
    GGCTGGATGA AGAACCAGCC CCTTTCAGGT GGGGAGATAT CTTCCAAAAC TGAACTGGAT
    GAATTGCAGG AAGAAGTGGC CAGAAGGGCA CAGGAACAGG AACTTCGAAG GAAACGAGAG
    AAGGAATTAG AGGCAGCTAA AGGGTTTAAC CCTCATCCCA GCCGCTTCAT GGACTTGGAT
    GAACTGCAGA ATCAGGGGAG GAGTGACGGC TTTGAGAGGT CCCTGCAAGA GGCAGATTCA
    GTGTTTGAAG AGTCGCTGCA TCTGGAGCAG AAGGGAGACT GTGCTGCCGC TTTGGCTCTC
    TGTAACGAAG CTATCTCTAA ACTAAGACTT GCCCTGCATG GTGCCAGCTC TAGCACGCAC
    AGCAGAGCCC TAGTCGATAA GAAGTTGCAA ATCAGTATTC GAAAAGCACG GAGCCTGCAG
    GATCGCATGC AGCAGCAGCA ATCATCTCAG CCGCCGTCGC AGCCCTCAAC CTGCCTCCCC
    TCACAGGGAG GAGCCCTTCC TCAGCCAACA AGTGAACAGC CTATCCCGCT CCAAGTATTG
    TTAAGCCAGG AGGCCCAACT GGAACCCTAC ACGAATACGG AGTTTGGGGC CAGTACTTTC
    ATTTACTCAC CTGCTTCCTG CCATGAGCCA CACTCGCCAT TATTCCCAGA GTCATCCGCC
    CTGTCTGCTC CACAGCACAA TTCCCCCAGT GGGTCTGCCT CGAACCTTCT TACTTCTGTT
    GAGGTGGACA GTATTGACCC CTCTGTGTTC CACAGGCAGG GCTTACCTAA AACACCAGGG
    AGACCTGAGA AGAGTTCTCA TCATGGTTGG GAATCAGAGG ATGAGCTGCA AAGCTGCAGG
    GGTGAAAGCA GCAGCTGCGC CAGGTCCCCC CAACGAGAAG GAAGAGACAC TGATCGAGAA
    CAGCTATTCC GAGAAAAGAG GGATCCTGCT GACTCATCCC AGGTGATGCC TTGGCCACAG
    CCAATTGGAA TAATAGCCAC CTCTGAGAGA GGAGAGGCAC ACAGTCTAGG CTGTGGTCCT
    TCAAATTCAG CAGCTCAGCC TAGCCTTTCT CTGTACAGGG CCTGCCACCC CGTAAAACCT
    GCTCCTTCCT CATCTGTGCT TCACTCCTCT AATACTGTAC AGAAAACTAA CCAATGCCTC
    CAAACCCAAA ACTTCAAAAT TCCATTGACT TCAAATATGG ATCGAGGCAG CGAGGAGCCC
    TCTATGCCAG AGTTTCCTAC CACCAAGGGG CTTGTCCGCT CTTTGGCAGA GCAGTTCCAG
    AGGATGCACG GAGCCTCCAC CAGGGATGGT ACAGGTGCCC AGGATAGAAG TTTGCCAAAC
    AGTCTAAGGA AGGACTCTCC ACCTTCCGAC TGTAAGCCTC CTTTCCCACA GAGTCAAGGG
    AAAGGCCACT GGTCTTGGGC AAAACAACAA CCCTCTTTGG ATGGTAGGAA CAAACTGCCT
    TCCTGGGAAG AGCCTGCTAA TCATCCTTCT CTTGCCACGA ACTCTGGGCC GCCTAATGGT
    GAAGCTTCTA GGGGAGGACA GCCCCGGTTG GCAGAGTCAG GTATTCACCA CGGGAAACCG
    TCTCAAGTGA GAGATGCTGG GCCTAAGGAG CTGGGCAGCA GCGTCGACTC GGGCACTTCC
    TTGCCTTCGG ATTCTTGGGT GAATGTCACA AGGCTCTGTG ATTCTCAGCT TAAACATGGG
    GTCCCTGGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACCTGTGT AACCTATCCA
    GAAAGAAACC ACATCATTTT GCATCCACAT TGGAACCAGG ACACAGAGCA GGAGACCTCA
    GAATTGGAGT CTCTCTACCA AGCCAGTCTT CAGGTTTCCC AATCTGGCTG TTCTGGATGT
    GGGCAGCAGG AAGTAGCCTG GAACCCGCTG AGCCAAACAG GCTCTGCAGA TGGTGTAGGG
    AGGAGGTTGC ACTCAGCCCC TGGCCTTGGT CTCTCAAAGA CCCCAACAGC AGAAATGGAG
    CACAGTCTCC ATGAAGCCAA CTCAGTGTCT GCTTCTCAGG CCGCACCTTG CCAAGGCCCC
    AGCCGGGAAT GTGGGGAGGA TGATCAGTAT GGTGCAGAGA ACTTTCGGCG CATCTCACGC
    AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAGGTCC CAGTCTCGTC CCACAGTTTT
    GATTCATCAA GCGTGAGGAA AAAGCCTTTG GAAACCCGGC ATCGTTGTTC CAGCTCCTCT
    TCCCTCCCTG TCATCCATGA CCCTCCTGTA TTTCTCCTTG GTCCCCAACT CTACCCACCC
    CAACCACAGT TCCTGTCCCC AGATGTCTTG ATGCCCAGCA TGGCAGGGGA GCCCCATAGA
    CCCCCAGGAA CTTCGAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT
    TCCGAAGGGG TCAAGTACAC AGGAAAGACT TTGAACTACC GGAGTCTCCC CCATCGTTCC
    AGAACAGATG CCTCCCGGGG ACTGTGGTCA GAGGCCAACC AGCATGTTGG GGCCAGATTC
    CTGACTACTC CAGGGTGCAA GCCTCAGCTA ACCCACACTG CCACGCTACC AGAAAGACAC
    CAGGGCCTTC AGGTTCCTCA TGCTCAGTCC TGGGGGGATC TTTTCCATTC ACCCTCCCAC
    CCTCCTGTTG TTCACCCTGT GTCGCCACCA TCAAGCAGTC TTTGTGTACC CCTGAGGTCA
    GCTTGGAATT CAGGTCCTGT TCCAGGGTTT CGAGCCCCTG GTCCTCGAAG AGTAGATATG
    CCCCCGGATG ATGACTGGAG GCAAAGCAGT TATGCCCTGC CATCTAGGCA CAGGAGGACA
    GGGAGAGAGG AGTTTCTCTT TGTCCTAGCA GATGCCCCTA ACAGAGAACA GATCAGGGCT
    AGAGCCCTGC AGCACAGCAG GTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59795
    Clone ID Related Accession (Same CDS sequence) XM_024986831.1
    Accession Version XM_024986831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC AGTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842599.1
    CDS883..5664
    Translation

    Target ORF information:

    RefSeq Version XM_024986831.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC AGTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005199533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199590.2
    CDS1394..6463
    Translation

    Target ORF information:

    RefSeq Version XM_005199533.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005199537.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199594.2
    CDS812..5881
    Translation

    Target ORF information:

    RefSeq Version XM_005199537.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199537.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_002705400.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002705400.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002705446.3
    CDS880..5949
    Translation

    Target ORF information:

    RefSeq Version XM_002705400.5
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002705400.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_015469846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325332.1
    CDS729..5798
    Translation

    Target ORF information:

    RefSeq Version XM_015469846.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005226500.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226557.1
    CDS1394..6463
    Translation

    Target ORF information:

    RefSeq Version XM_005226500.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005226504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226561.1
    CDS812..5881
    Translation

    Target ORF information:

    RefSeq Version XM_005226504.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005226503.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226560.1
    CDS880..5949
    Translation

    Target ORF information:

    RefSeq Version XM_005226503.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226503.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_003587990.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003588038.1
    CDS882..5951
    Translation

    Target ORF information:

    RefSeq Version XM_003587990.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587990.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005199534.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199591.2
    CDS927..5996
    Translation

    Target ORF information:

    RefSeq Version XM_005199534.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199534.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005226501.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226558.1
    CDS927..5996
    Translation

    Target ORF information:

    RefSeq Version XM_005226501.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226501.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_015461045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316531.1
    CDS729..5798
    Translation

    Target ORF information:

    RefSeq Version XM_015461045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34295
    Clone ID Related Accession (Same CDS sequence) XM_005199533.2 , XM_005199537.3 , XM_002705400.5 , XM_015469846.1 , XM_005226500.2 , XM_005226504.3 , XM_005226503.3 , XM_003587990.4 , XM_005199534.3 , XM_005226501.3 , XM_015461045.1 , XM_005199536.3
    Accession Version XM_005199536.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199593.2
    CDS882..5951
    Translation

    Target ORF information:

    RefSeq Version XM_005199536.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199536.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34296
    Clone ID Related Accession (Same CDS sequence) XM_005226505.2 , XM_005199538.2
    Accession Version XM_005226505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226562.1
    CDS1394..6343
    Translation

    Target ORF information:

    RefSeq Version XM_005226505.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34296
    Clone ID Related Accession (Same CDS sequence) XM_005226505.2 , XM_005199538.2
    Accession Version XM_005199538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199595.2
    CDS1394..6343
    Translation

    Target ORF information:

    RefSeq Version XM_005199538.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGATTCAT CAAGCGTGAG GAAAAAGCCT TTGGAAACCC GGCATCGTTG TTCCAGCTCC
    TCTTCCCTCC CTGTCATCCA TGACCCTCCT GTATTTCTCC TTGGTCCCCA ACTCTACCCA
    CCCCAACCAC AGTTCCTGTC CCCAGATGTC TTGATGCCCA GCATGGCAGG GGAGCCCCAT
    AGACCCCCAG GAACTTCGAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA
    ACTTCCGAAG GGGTCAAGTA CACAGGAAAG ACTTTGAACT ACCGGAGTCT CCCCCATCGT
    TCCAGAACAG ATGCCTCCCG GGGACTGTGG TCAGAGGCCA ACCAGCATGT TGGGGCCAGA
    TTCCTGACTA CTCCAGGGTG CAAGCCTCAG CTAACCCACA CTGCCACGCT ACCAGAAAGA
    CACCAGGGCC TTCAGGTTCC TCATGCTCAG TCCTGGGGGG ATCTTTTCCA TTCACCCTCC
    CACCCTCCTG TTGTTCACCC TGTGTCGCCA CCATCAAGCA GTCTTTGTGT ACCCCTGAGG
    TCAGCTTGGA ATTCAGGTCC TGTTCCAGGG TTTCGAGCCC CTGGTCCTCG AAGAGTAGAT
    ATGCCCCCGG ATGATGACTG GAGGCAAAGC AGTTATGCCC TGCCATCTAG GCACAGGAGG
    ACAGGGAGAG AGGAGTTTCT CTTTGTCCTA GCAGATGCCC CTAACAGAGA ACAGATCAGG
    GCTAGAGCCC TGCAGCACAG CAGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34297
    Clone ID Related Accession (Same CDS sequence) XM_005226506.2 , XM_005199539.2
    Accession Version XM_005226506.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCTTT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226563.1
    CDS1389..6287
    Translation

    Target ORF information:

    RefSeq Version XM_005226506.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCTTT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34297
    Clone ID Related Accession (Same CDS sequence) XM_005226506.2 , XM_005199539.2
    Accession Version XM_005199539.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCTTT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199596.2
    CDS1389..6287
    Translation

    Target ORF information:

    RefSeq Version XM_005199539.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199539.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACTCC CATCCAAGAA AGGGTCATTG
    ATAGAGCGCA AGAGGAGCTC TGGCCGGGTC AGGAGGAAAG GCGATGAGTC TCAGGCCTCA
    GGGTACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCCAAA
    TCATCCAGCA GCACCAACAG GCTAAGGGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT
    AACAGACCAT CCCTGGCTTC TCAGACCACC CTAGGACCTC CTTGTGTAGG GAAGGGAGGA
    GAACAGACGG ACAAAAAACC TCCTCGGAGC CTGCCTTTAC ACTCTCGTGA CTGGGAAGTA
    GAGAGTACCA GCAGCGAGTC AAAATCCAGT TCTTCTAGCA AATATCGTCC AACATGGAGA
    CCCAAACGGG AGTCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACAGT
    GGCTATACCC AGCTTAGCCC CTTTTCTGAA GATTCAGCAA AAGAATTTAC ACCAGATGAG
    TCAAGCAAGC CGCCTGCTTA TGACATTAGA ACTGGACCAA GCCCCCAGTA CAAGATCTGG
    GGCCCAGCAC GGCCAGGCTG TCACTTTTTA GAGCAACACC CTCGCCTCAT CCAGCGAATG
    GAATCTGGCT ACGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC
    CTGCCTGAGA GCTCAAGTGT CTGCAGGGAT CCAAGTGCTA AGAGGTCAGC AGGATTGGCC
    CCTTCCTGGC GTCACATCCC TAAGTCTCAC AGCAGCAGCA TCCTGGAGGC AGACTCTGCC
    GTGTCCAGGA GTGGCTGGAT GAAGAACCAG CCCCTTTCAG GTGGGGAGAT ATCTTCCAAA
    ACTGAACTGG ATGAATTGCA GGAAGAAGTG GCCAGAAGGG CACAGGAACA GGAACTTCGA
    AGGAAACGAG AGAAGGAATT AGAGGCAGCT AAAGGGTTTA ACCCTCATCC CAGCCGCTTC
    ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA
    GAGGCAGATT CAGTGTTTGA AGAGTCGCTG CATCTGGAGC AGAAGGGAGA CTGTGCTGCC
    GCTTTGGCTC TCTGTAACGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC
    TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA
    CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCATCTC AGCCGCCGTC GCAGCCCTCA
    ACCTGCCTCC CCTCACAGGG AGGAGCCCTT CCTCAGCCAA CAAGTGAACA GCCTATCCCG
    CTCCAAGTAT TGTTAAGCCA GGAGGCCCAA CTGGAACCCT ACACGAATAC GGAGTTTGGG
    GCCAGTACTT TCATTTACTC ACCTGCTTCC TGCCATGAGC CACACTCGCC ATTATTCCCA
    GAGTCATCCG CCCTGTCTGC TCCACAGCAC AATTCCCCCA GTGGGTCTGC CTCGAACCTT
    CTTACTTCTG TTGAGGTGGA CAGTATTGAC CCCTCTGTGT TCCACAGGCA GGGCTTACCT
    AAAACACCAG GGAGACCTGA GAAGAGTTCT CATCATGGTT GGGAATCAGA GGATGAGCTG
    CAAAGCTGCA GGGGTGAAAG CAGCAGCTGC GCCAGGTCCC CCCAACGAGA AGGAAGAGAC
    ACTGATCGAG AACAGCTATT CCGAGAAAAG AGGGATCCTG CTGACTCATC CCAGGTGATG
    CCTTGGCCAC AGCCAATTGG AATAATAGCC ACCTCTGAGA GAGGAGAGGC ACACAGTCTA
    GGCTGTGGTC CTTCAAATTC AGCAGCTCAG CCTAGCCTTT CTCTGTACAG GGCCTGCCAC
    CCCGTAAAAC CTGCTCCTTC CTCATCTGTG CTTCACTCCT CTAATACTGT ACAGAAAACT
    AACCAATGCC TCCAAACCCA AAACTTCAAA ATTCCATTGA CTTCAAATAT GGATCGAGGC
    AGCGAGGAGC CCTCTATGCC AGAGTTTCCT ACCACCAAGG GGCTTGTCCG CTCTTTGGCA
    GAGCAGTTCC AGAGGATGCA CGGAGCCTCC ACCAGGGATG GTACAGGTGC CCAGGATAGA
    AGTTTGCCAA ACAGTCTAAG GAAGGACTCT CCACCTTCCG ACTGTAAGCC TCCTTTCCCA
    CAGAGTCAAG GGAAAGGCCA CTGGTCTTGG GCAAAACAAC AACCCTCTTT GGATGGTAGG
    AACAAACTGC CTTCCTGGGA AGAGCCTGCT AATCATCCTT CTCTTGCCAC GAACTCTGGG
    CCGCCTAATG GTGAAGCTTC TAGGGGAGGA CAGCCCCGGT TGGCAGAGTC AGGTATTCAC
    CACGGGAAAC CGTCTCAAGT GAGAGATGCT GGGCCTAAGG AGCTGGGCAG CAGCGTCGAC
    TCGGGCACTT CCTTGCCTTC GGATTCTTGG GTGAATGTCA CAAGGCTCTG TGATTCTCAG
    CTTAAACATG GGGTCCCTGG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACCTGT
    GTAACCTATC CAGAAAGAAA CCACATCATT TTGCATCCAC ATTGGAACCA GGACACAGAG
    CAGGAGACCT CAGAATTGGA GTCTCTCTAC CAAGCCAGTC TTCAGGTTTC CCAATCTGGC
    TGTTCTGGAT GTGGGCAGCA GGAAGTAGCC TGGAACCCGC TGAGCCAAAC AGGCTCTGCA
    GATGGTGTAG GGAGGAGGTT GCACTCAGCC CCTGGCCTTG GTCTCTCAAA GACCCCAACA
    GCAGAAATGG AGCACAGTCT CCATGAAGCC AACTCAGTGT CTGCTTCTCA GGCCGCACCT
    TGCCAAGGCC CCAGCCGGGA ATGTGGGGAG GATGATCTTT ATGGTGCAGA GAACTTTCGG
    CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAGGT CCCAGTCTCG
    TCCCACAGTT TTGATTCATC AAGCGTGAGG AAAAAGCCTT TGGAAACCCG GCATCGTTGT
    TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GACCCTCCTG TATTTCTCCT TGGTCCCCAA
    CTCTACCCAC CCCAACCACA GTTCCTGTCC CCAGATGTCT TGATGCCCAG CATGGCAGGG
    GAGCCCCATA GACCCCCAGG AACTTCGAGG AGTGTCCAGC AGTTTCTGGC TATGTGTGAC
    AGGGGTGAAA CTTCCGAAGG GGTCAAGTAC ACAGGAAAGA CTTTGAACTA CCGGAGTCTC
    CCCCATCGTT CCAGAACAGA TGCCTCCCGG GGACTGTGGT CAGAGGCCAA CCAGCATGTT
    GGGGCCAGAT TCCTGACTAC TCCAGGGTGC AAGCCTCAGC TAACCCACAC TGCCACGCTA
    CCAGAAAGAC ACCAGGGCCT TCAGGTTCCT CATGCTCAGT CCTGGGGGGA TCTTTTCCAT
    TCACCCTCCC ACCCTCCTGT TGTTCACCCT GTGTCGCCAC CATCAAGCAG TCTTTGTGTA
    CCCCTGAGGT CAGCTTGGAA TTCAGGTCCT GTTCCAGGGT TTCGAGCCCC TGGTCCTCGA
    AGAGTAGATA TGCCCCCGGA TGATGACTGG AGGCAAAGCA GTTATGCCCT GCCATCTAGG
    CACAGGAGGA CAGGGAGAGA GGAGTTTCTC TTTGTCCTAG CAGATGCCCC TAACAGAGAA
    CAGATCAGGG CTAGAGCCCT GCAGCACAGC AGGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59797
    Clone ID Related Accession (Same CDS sequence) XM_010799131.2 , XM_010820659.2
    Accession Version XM_010799131.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC TTTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010797433.2
    CDS1393..6174
    Translation

    Target ORF information:

    RefSeq Version XM_010799131.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010799131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC TTTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59797
    Clone ID Related Accession (Same CDS sequence) XM_010799131.2 , XM_010820659.2
    Accession Version XM_010820659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC TTTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818961.1
    CDS1393..6174
    Translation

    Target ORF information:

    RefSeq Version XM_010820659.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GCTAAACTAA GACTTGCCCT GCATGGTGCC
    AGCTCTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA
    GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAT CTCAGCCGCC GTCGCAGCCC
    TCAACCTGCC TCCCCTCACA GGGAGGAGCC CTTCCTCAGC CAACAAGTGA ACAGCCTATC
    CCGCTCCAAG TATTGTTAAG CCAGGAGGCC CAACTGGAAC CCTACACGAA TACGGAGTTT
    GGGGCCAGTA CTTTCATTTA CTCACCTGCT TCCTGCCATG AGCCACACTC GCCATTATTC
    CCAGAGTCAT CCGCCCTGTC TGCTCCACAG CACAATTCCC CCAGTGGGTC TGCCTCGAAC
    CTTCTTACTT CTGTTGAGGT GGACAGTATT GACCCCTCTG TGTTCCACAG GCAGGGCTTA
    CCTAAAACAC CAGGGAGACC TGAGAAGAGT TCTCATCATG GTTGGGAATC AGAGGATGAG
    CTGCAAAGCT GCAGGGGTGA AAGCAGCAGC TGCGCCAGGT CCCCCCAACG AGAAGGAAGA
    GACACTGATC GAGAACAGCT ATTCCGAGAA AAGAGGGATC CTGCTGACTC ATCCCAGGTG
    ATGCCTTGGC CACAGCCAAT TGGAATAATA GCCACCTCTG AGAGAGGAGA GGCACACAGT
    CTAGGCTGTG GTCCTTCAAA TTCAGCAGCT CAGCCTAGCC TTTCTCTGTA CAGGGCCTGC
    CACCCCGTAA AACCTGCTCC TTCCTCATCT GTGCTTCACT CCTCTAATAC TGTACAGAAA
    ACTAACCAAT GCCTCCAAAC CCAAAACTTC AAAATTCCAT TGACTTCAAA TATGGATCGA
    GGCAGCGAGG AGCCCTCTAT GCCAGAGTTT CCTACCACCA AGGGGCTTGT CCGCTCTTTG
    GCAGAGCAGT TCCAGAGGAT GCACGGAGCC TCCACCAGGG ATGGTACAGG TGCCCAGGAT
    AGAAGTTTGC CAAACAGTCT AAGGAAGGAC TCTCCACCTT CCGACTGTAA GCCTCCTTTC
    CCACAGAGTC AAGGGAAAGG CCACTGGTCT TGGGCAAAAC AACAACCCTC TTTGGATGGT
    AGGAACAAAC TGCCTTCCTG GGAAGAGCCT GCTAATCATC CTTCTCTTGC CACGAACTCT
    GGGCCGCCTA ATGGTGAAGC TTCTAGGGGA GGACAGCCCC GGTTGGCAGA GTCAGGTATT
    CACCACGGGA AACCGTCTCA AGTGAGAGAT GCTGGGCCTA AGGAGCTGGG CAGCAGCGTC
    GACTCGGGCA CTTCCTTGCC TTCGGATTCT TGGGTGAATG TCACAAGGCT CTGTGATTCT
    CAGCTTAAAC ATGGGGTCCC TGGGCCAGGA ATGAAGTCCT CCCCTCATGA TTCCCATACC
    TGTGTAACCT ATCCAGAAAG AAACCACATC ATTTTGCATC CACATTGGAA CCAGGACACA
    GAGCAGGAGA CCTCAGAATT GGAGTCTCTC TACCAAGCCA GTCTTCAGGT TTCCCAATCT
    GGCTGTTCTG GATGTGGGCA GCAGGAAGTA GCCTGGAACC CGCTGAGCCA AACAGGCTCT
    GCAGATGGTG TAGGGAGGAG GTTGCACTCA GCCCCTGGCC TTGGTCTCTC AAAGACCCCA
    ACAGCAGAAA TGGAGCACAG TCTCCATGAA GCCAACTCAG TGTCTGCTTC TCAGGCCGCA
    CCTTGCCAAG GCCCCAGCCG GGAATGTGGG GAGGATGATC TTTATGGTGC AGAGAACTTT
    CGGCGCATCT CACGCAGTCT CAGTGGCACC GTTGTCTCAG AGAGGGAGGA GGTCCCAGTC
    TCGTCCCACA GTTTTGATTC ATCAAGCGTG AGGAAAAAGC CTTTGGAAAC CCGGCATCGT
    TGTTCCAGCT CCTCTTCCCT CCCTGTCATC CATGACCCTC CTGTATTTCT CCTTGGTCCC
    CAACTCTACC CACCCCAACC ACAGTTCCTG TCCCCAGATG TCTTGATGCC CAGCATGGCA
    GGGGAGCCCC ATAGACCCCC AGGAACTTCG AGGAGTGTCC AGCAGTTTCT GGCTATGTGT
    GACAGGGGTG AAACTTCCGA AGGGGTCAAG TACACAGGAA AGACTTTGAA CTACCGGAGT
    CTCCCCCATC GTTCCAGAAC AGATGCCTCC CGGGGACTGT GGTCAGAGGC CAACCAGCAT
    GTTGGGGCCA GATTCCTGAC TACTCCAGGG TGCAAGCCTC AGCTAACCCA CACTGCCACG
    CTACCAGAAA GACACCAGGG CCTTCAGGTT CCTCATGCTC AGTCCTGGGG GGATCTTTTC
    CATTCACCCT CCCACCCTCC TGTTGTTCAC CCTGTGTCGC CACCATCAAG CAGTCTTTGT
    GTACCCCTGA GGTCAGCTTG GAATTCAGGT CCTGTTCCAG GGTTTCGAGC CCCTGGTCCT
    CGAAGAGTAG ATATGCCCCC GGATGATGAC TGGAGGCAAA GCAGTTATGC CCTGCCATCT
    AGGCACAGGA GGACAGGGAG AGAGGAGTTT CTCTTTGTCC TAGCAGATGC CCCTAACAGA
    GAACAGATCA GGGCTAGAGC CCTGCAGCAC AGCAGGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59798
    Clone ID Related Accession (Same CDS sequence) XM_010820660.2 , XM_010799132.2
    Accession Version XM_010820660.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGAACTTC GAGGAGTGTC CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCG
    AAGGGGTCAA GTACACAGGA AAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818962.1
    CDS1420..5829
    Translation

    Target ORF information:

    RefSeq Version XM_010820660.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820660.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGAACTTC GAGGAGTGTC CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCG
    AAGGGGTCAA GTACACAGGA AAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59798
    Clone ID Related Accession (Same CDS sequence) XM_010820660.2 , XM_010799132.2
    Accession Version XM_010799132.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGAACTTC GAGGAGTGTC CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCG
    AAGGGGTCAA GTACACAGGA AAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010797434.2
    CDS1420..5829
    Translation

    Target ORF information:

    RefSeq Version XM_010799132.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010799132.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGCCGTGGTA GTGTCCAGGG GATGTTTGCT 
    CCGCGAAGCT CAACCTCCAT AGCTCCCAGC AAAGGCCTCA GCAATGAGCC AGGACAAAAC
    AGCTGCTTCC TCAACAGTGC CTTGCAGGTT TTATGGCACT TGGACATCTT CCGACGTAGC
    TTTAGGCAGC TTACAACTCA CAAATGCATG GGAGATTCCT GCATCTTTTG TGCTCTCAAG
    GGAATCTTTA ACCAGTTTCA GTGCAGTAGT GAAAAAGTGC TTCCATCTGA CGCTCTCCGC
    AGTGCTCTGG CAAAGACTTT TCAGGATGAG CAGCGTTTCC AGCTGGGAAT TATGGATGAT
    GCCGCGGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC
    AAAGAGGACA TATGTACTGC CCAGCACTGC ATCTCCCACC AGAAGTTTGC GATGACACTG
    TTCGAGCAGT GCGTATGTAC CAGCTGTGGC GCCACTTCTG ACCCGCTGCC TTTCATCCAG
    ATGGTGCATT ATATCTCCAC TACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA
    CGAGAAAAAC CCTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG
    GATCTGCGGA ACTGCCCGAG CAACTGTGGA GAAAGGATTC GGATTCGCCG TGTGTTGATG
    AACGCACCAC AGATTATCAC TATTGGGCTG GTATGGGACT CGGACCACTC AGACTTGGCA
    GAGGATGTCA TTCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG
    ACAGATGACC GGGCTAAGCA GTCTGAACTG TACTTAGTAG GAATGATCTG TTACTACGGC
    AAACATTACT CTACATTCTT TTTCCAAACA AAAATCCGCA AATGGATGTA TTTTGATGAT
    GCTCATGTCA AGGAGATTGG GCCCAAGTGG AAGGATGTGG TGACCAAATG CATCAAGGGG
    CATTATCAGC CTTTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT GTCCACCCAG
    GACTTGCCCC CCCAAGTTGA GTTCCAGTCG TACAGCAGGA CATGCTACGA TAGTGAAGAT
    TCAGGGAGGG AGCCCTCCAT CTCAAGTGAT ACTCGAACAG ATTCCTCAAC GGAGAGCTAT
    CCCTACAAAC ACTTCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG
    ACAGTCATCT ATAATGTGGA GACTGATTCC ACGTCTCAGA GCAGTCGGGA CACAGGACAC
    CTGACTGATA GTGAATGTAA TCAGAAACTC CCATCCAAGA AAGGGTCATT GATAGAGCGC
    AAGAGGAGCT CTGGCCGGGT CAGGAGGAAA GGCGATGAGT CTCAGGCCTC AGGGTACCAC
    AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCCAA ATCATCCAGC
    AGCACCAACA GGCTAAGGGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA
    TCCCTGGCTT CTCAGACCAC CCTAGGACCT CCTTGTGTAG GGAAGGGAGG AGAACAGACG
    GACAAAAAAC CTCCTCGGAG CCTGCCTTTA CACTCTCGTG ACTGGGAAGT AGAGAGTACC
    AGCAGCGAGT CAAAATCCAG TTCTTCTAGC AAATATCGTC CAACATGGAG ACCCAAACGG
    GAGTCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACAG TGGCTATACC
    CAGCTTAGCC CCTTTTCTGA AGATTCAGCA AAAGAATTTA CACCAGATGA GTCAAGCAAG
    CCGCCTGCTT ATGACATTAG AACTGGACCA AGCCCCCAGT ACAAGATCTG GGGCCCAGCA
    CGGCCAGGCT GTCACTTTTT AGAGCAACAC CCTCGCCTCA TCCAGCGAAT GGAATCTGGC
    TACGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGCC TGGATGCAGC CCTGCCTGAG
    AGCTCAAGTG TCTGCAGGGA TCCAAGTGCT AAGAGGTCAG CAGGATTGGC CCCTTCCTGG
    CGTCACATCC CTAAGTCTCA CAGCAGCAGC ATCCTGGAGG CAGACTCTGC CGTGTCCAGG
    AGTGGCTGGA TGAAGAACCA GCCCCTTTCA GGTGGGGAGA TATCTTCCAA AACTGAACTG
    GATGAATTGC AGGAAGAAGT GGCCAGAAGG GCACAGGAAC AGGAACTTCG AAGGAAACGA
    GAGAAGGAAT TAGAGGCAGC TAAAGGGTTT AACCCTCATC CCAGCCGCTT CATGGACTTG
    GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAGA GGTCCCTGCA AGAGGCAGAT
    TCAGTGTTTG AAGAGTCGCT GCATCTGGAG CAGAAGGGAG ACTGTGCTGC CGCTTTGGCT
    CTCTGTAACG AAGCTATCTC TAAACTAAGA CTTGCCCTGC ATGGTGCCAG CTCTAGCACG
    CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGGAGCCTG
    CAGGATCGCA TGCAGCAGCA GCAATCATCT CAGCCGCCGT CGCAGCCCTC AACCTGCCTC
    CCCTCACAGG GAGGAGCCCT TCCTCAGCCA ACAAGTGAAC AGCCTATCCC GCTCCAAGTA
    TTGTTAAGCC AGGAGGCCCA ACTGGAACCC TACACGAATA CGGAGTTTGG GGCCAGTACT
    TTCATTTACT CACCTGCTTC CTGCCATGAG CCACACTCGC CATTATTCCC AGAGTCATCC
    GCCCTGTCTG CTCCACAGCA CAATTCCCCC AGTGGGTCTG CCTCGAACCT TCTTACTTCT
    GTTGAGGTGG ACAGTATTGA CCCCTCTGTG TTCCACAGGC AGGGCTTACC TAAAACACCA
    GGGAGACCTG AGAAGAGTTC TCATCATGGT TGGGAATCAG AGGATGAGCT GCAAAGCTGC
    AGGGGTGAAA GCAGCAGCTG CGCCAGGTCC CCCCAACGAG AAGGAAGAGA CACTGATCGA
    GAACAGCTAT TCCGAGAAAA GAGGGATCCT GCTGACTCAT CCCAGGTGAT GCCTTGGCCA
    CAGCCAATTG GAATAATAGC CACCTCTGAG AGAGGAGAGG CACACAGTCT AGGCTGTGGT
    CCTTCAAATT CAGCAGCTCA GCCTAGCCTT TCTCTGTACA GGGCCTGCCA CCCCGTAAAA
    CCTGCTCCTT CCTCATCTGT GCTTCACTCC TCTAATACTG TACAGAAAAC TAACCAATGC
    CTCCAAACCC AAAACTTCAA AATTCCATTG ACTTCAAATA TGGATCGAGG CAGCGAGGAG
    CCCTCTATGC CAGAGTTTCC TACCACCAAG GGGCTTGTCC GCTCTTTGGC AGAGCAGTTC
    CAGAGGATGC ACGGAGCCTC CACCAGGGAT GGTACAGGTG CCCAGGATAG AAGTTTGCCA
    AACAGTCTAA GGAAGGACTC TCCACCTTCC GACTGTAAGC CTCCTTTCCC ACAGAGTCAA
    GGGAAAGGCC ACTGGTCTTG GGCAAAACAA CAACCCTCTT TGGATGGTAG GAACAAACTG
    CCTTCCTGGG AAGAGCCTGC TAATCATCCT TCTCTTGCCA CGAACTCTGG GCCGCCTAAT
    GGTGAAGCTT CTAGGGGAGG ACAGCCCCGG TTGGCAGAGT CAGGTATTCA CCACGGGAAA
    CCGTCTCAAG TGAGAGATGC TGGGCCTAAG GAGCTGGGCA GCAGCGTCGA CTCGGGCACT
    TCCTTGCCTT CGGATTCTTG GGTGAATGTC ACAAGGCTCT GTGATTCTCA GCTTAAACAT
    GGGGTCCCTG GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACCTG TGTAACCTAT
    CCAGAAAGAA ACCACATCAT TTTGCATCCA CATTGGAACC AGGACACAGA GCAGGAGACC
    TCAGAATTGG AGTCTCTCTA CCAAGCCAGT CTTCAGGTTT CCCAATCTGG CTGTTCTGGA
    TGTGGGCAGC AGGAAGTAGC CTGGAACCCG CTGAGCCAAA CAGGCTCTGC AGATGGTGTA
    GGGAGGAGGT TGCACTCAGC CCCTGGCCTT GGTCTCTCAA AGACCCCAAC AGCAGAAATG
    GAGCACAGTC TCCATGAAGC CAACTCAGTG TCTGCTTCTC AGGCCGCACC TTGCCAAGGC
    CCCAGCCGGG AATGTGGGGA GGATGATCTT TATGGTGCAG AGAACTTTCG GCGCATCTCA
    CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAGG TCCCAGTCTC GTCCCACAGT
    TTTGAACTTC GAGGAGTGTC CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCG
    AAGGGGTCAA GTACACAGGA AAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.