USP54A cDNA ORF clone, Danio rerio(Zebrafish)

The following USP54A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP54A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa18804 XM_021480613.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa54717 XM_017358771.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa44212 XM_021480612.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa54715 XM_017358769.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa54716 XM_017358770.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa18803 NM_001201566.2
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18803 XM_009307335.2
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54714 XM_017358768.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa54714 XM_017358767.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa54714 XM_017358766.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa18802 NM_001327875.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa18802 XM_021480611.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa18802 XM_021480610.1
Latest version!
Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa18804
    Clone ID Related Accession (Same CDS sequence) XM_021480613.1
    Accession Version XM_021480613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTTGA ACAGTCTGTC CCGGTCCAAA TACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336288.1
    CDS674..3172
    Translation

    Target ORF information:

    RefSeq Version XM_021480613.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTTGA ACAGTCTGTC CCGGTCCAAA TACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54717
    Clone ID Related Accession (Same CDS sequence) XM_017358771.1
    Accession Version XM_017358771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTTGAA CAGTCTGTCC CGGTCCAAAT ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214260.1
    CDS671..3178
    Translation

    Target ORF information:

    RefSeq Version XM_017358771.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTTGAA CAGTCTGTCC CGGTCCAAAT ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44212
    Clone ID Related Accession (Same CDS sequence) XM_021480612.1
    Accession Version XM_021480612.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336287.1
    CDS674..5047
    Translation

    Target ORF information:

    RefSeq Version XM_021480612.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480612.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54715
    Clone ID Related Accession (Same CDS sequence) XM_017358769.1
    Accession Version XM_017358769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214258.1
    CDS671..5053
    Translation

    Target ORF information:

    RefSeq Version XM_017358769.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54716
    Clone ID Related Accession (Same CDS sequence) XM_017358770.1
    Accession Version XM_017358770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGAGAC TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG
    GTGAAGCATT CTCCCGCGAA AACTATCACA ACGTCGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214259.1
    CDS671..4492
    Translation

    Target ORF information:

    RefSeq Version XM_017358770.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGAGAC TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG
    GTGAAGCATT CTCCCGCGAA AACTATCACA ACGTCGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18803
    Clone ID Related Accession (Same CDS sequence) XM_009307335.2 , NM_001201566.2
    Accession Version NM_001201566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649385.8 and BX649277.5. On May 27, 2016 this sequence version replaced NM_001201566.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGAGA CTGCCAAAAG ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT
    TCTCCCGCGA AAACTATCAC AACGTCGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188495.1
    CDS1..3813
    Translation

    Target ORF information:

    RefSeq Version NM_001201566.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGAGA CTGCCAAAAG ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT
    TCTCCCGCGA AAACTATCAC AACGTCGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18803
    Clone ID Related Accession (Same CDS sequence) XM_009307335.2 , NM_001201566.2
    Accession Version XM_009307335.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGAGA CTGCCAAAAG ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT
    TCTCCCGCGA AAACTATCAC AACGTCGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305610.1
    CDS671..4483
    Translation

    Target ORF information:

    RefSeq Version XM_009307335.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307335.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGAGA CTGCCAAAAG ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT
    TCTCCCGCGA AAACTATCAC AACGTCGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54714
    Clone ID Related Accession (Same CDS sequence) XM_017358767.1 , XM_017358768.1 , XM_017358766.1
    Accession Version XM_017358767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214256.1
    CDS629..5131
    Translation

    Target ORF information:

    RefSeq Version XM_017358767.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54714
    Clone ID Related Accession (Same CDS sequence) XM_017358767.1 , XM_017358768.1 , XM_017358766.1
    Accession Version XM_017358768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214257.1
    CDS580..5082
    Translation

    Target ORF information:

    RefSeq Version XM_017358768.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54714
    Clone ID Related Accession (Same CDS sequence) XM_017358767.1 , XM_017358768.1 , XM_017358766.1
    Accession Version XM_017358766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214255.1
    CDS671..5173
    Translation

    Target ORF information:

    RefSeq Version XM_017358766.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGGTGTG TACGCAGGTG AGGGCCGCAG TGAACTGGAC
    GAGCTTCAGG AGGAAGTGGC TCGCCGGGCC AGAGAGCAGG AGTTGCAGAG ACGAAAGGAA
    AAGGAGAAAG AGGCAGCCAT GGGCTTCAAT CCCAGACCCA GCAAGTTCAT GGATCTGGAT
    GAGCTCCAGA ATCAAGGGAA AGGGGAAGGC TTTGAGCGCT GTGTGTTGGA GGCAGAGTCT
    TTGTTAGATC AGTCTCTGAG GCTGGAACAG GCAGGAGAGG TGGCCGCCGC TCTGTCTGTG
    GTTAACGAAG CAATGTCTAA ACTCAGGCAT ACCTTGCATG AGAGCAGCGC TAACGCTCAG
    GGCAGGATGC AAAAATGCAT GAGGAGAGCA CGAGGTCTGC AGCAACGCAT GCAACAGAAG
    CAACAGGAGG AAGAGAAGGA GCAGAAACAG CCCCTCAGTG AACAGTCTGT CCCGGTCCAA
    ATACTTCTGA CAAATGTTAA AGAAGAGGAT GAGAAGGCCA TTTCAGAAGA TGCATTAAGA
    CCACCGTCAC CTCTCCACAT AAAACCTCAG TCTACAAGTA AACCGATTTC TGAAACCCCA
    GGCCCTCCTG TCCCCTCTTA CCATACAGAT ACATGTTCCC AGAAGGACAA TACAGGGGAC
    CACCCTAGTT CTCTTGATGT GACAGAAAAT GATCGGGGTC CTAGGGACCT TGTAATTAAC
    ACAAACTCTC TTGTACAAAC TGCTAGTAAA CGCAGTCCTG CCACTGTGCC TGTGATTGCA
    GCGAATGGCT GTGAGGCTTC CCTGCAGTCC TCCGTTTCGC TCAACACTCC TGCTTATTTC
    AGTGCCCGTC TCACACCTTC CACCACTGAT CACTCTGAAG AAGGTCTCCA GAATCCCCAT
    CGTCCTCAGT CTCTCCATGG CCTGTCCCAA GCACCCCATG CCTGTCAAAA ATCACTCCCT
    GTTCCCTCAC GTAATTGGTC CTGTCGCAGT CTGGAGCGTC CTGATAGTCG TCCCACCACG
    CTGGACTCTT CCATGTATTC TCCAGTAGAC CTCAGTTCCC CATCGTCCCC CTGTCCTCCA
    TTCCTGTCTT CACCAGTGCT GGAGCAGCGT CCAGCCGGCC CCATGCCTGT GGAACGTTGG
    GCAGAGAATG TCAATCGATA CTACAATTCC CAGAAGTGTG CGCGGTCGGG ACGTGACTCG
    CCCTGCGAGG AGCTGTCGGA GCTGGACTCA CTCTATCAGG CCAGCTTGAA GGCTCCCAGC
    ATACCTAGAG CCACACGGGG AACCAGCCCT CAGCTTGCCA ACAGATCAGG TCCTGCTGTT
    TCTCGTAAAC ACAGCACTGG ATCCTCTGCC ACTCTTGGCC GCTCCAAAAC CCCCACAGCA
    GAGATTGAGA GGAGTGCCTA CAGGACGCCA ACGTACAGCA CTAAACCACT TCAGAAGCCT
    GTTTCTGCTG CAGCCTATAA TGAACCGCTG GGTGAGGATG AATCATACAG TGCTGAGAAC
    CTGCGACGAA TTGCTCGGAG TCTCAGTGGG ACTGTTGTTG GAGGACGGAC TGACGTACCC
    CCATCTCGCA GCTTTGACCC TCATGTCTCC AGGATGTCCA AGCATGTGAA TGTGCAACTC
    CCCACACATC GCTCCAGCAC ATCCCTGCAT GTTTTACCAC TTCCCTCCCA CAATCCACCA
    ATGTTCCCTC ACGAACCACC GCCCCAACAC CTTCACTCGA GCCACCGTCC TCACCCCGTC
    CCTACATCAT CACACCTGCA CCAGCAGACA CTGAGGCCCC CTCATGGACA AACAGGAGAC
    TGCCAAAAGA CCAACAAGTT TCTGGCTGTG CTTGACCGGG GTGAAGCATT CTCCCGCGAA
    AACTATCACA ACGTCGCACT GAGCTACGGC ACTCTTCCTC GGGCCCCTCG TAGGTCAGTT
    TCATCTGGGC CCCCAGTGTA CCAAATGAGG CCAGGATCCT TACCCCGAAA CCACCCAGAC
    GCAGCCTACG CCACACTATC CCGCCCTCAC CGCACTACAG CACCAAGTAG GACGATGGAT
    GGATTTTACA GGCAACCCCA CCAACACCCT TCATTAACAG CTCCAATCCC GCATCACTCC
    AGCCGTCATC ACCCGCACAC AGAGCCGCCA ACTCAACCCC TCCGCCTCGA TGTTCCCCCT
    GAGAGAGACT GGAGAGCTGC TAGGGACACT CACGTCTTCC CCAGAACAGA TCCCCGTGGA
    GGGACGTCCC GTGGCCATCC TGTCTATATC TGCGGGCTGT GCAAACAGAA CCCGGCTGAA
    CCCACTAGAA GCTACTGCCA AGCCTGCAGA GCCCATATGA GCCACTACAG GATGACCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18802
    Clone ID Related Accession (Same CDS sequence) XM_021480611.1 , XM_021480610.1 , NM_001327875.1
    Accession Version NM_001327875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649385.8 and BX649277.5. On May 27, 2016 this sequence version replaced XM_005156862.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001314804.1
    CDS687..5180
    Translation

    Target ORF information:

    RefSeq Version NM_001327875.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001327875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18802
    Clone ID Related Accession (Same CDS sequence) XM_021480611.1 , XM_021480610.1 , NM_001327875.1
    Accession Version XM_021480611.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336286.1
    CDS583..5076
    Translation

    Target ORF information:

    RefSeq Version XM_021480611.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480611.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18802
    Clone ID Related Accession (Same CDS sequence) XM_021480611.1 , XM_021480610.1 , NM_001327875.1
    Accession Version XM_021480610.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336285.1
    CDS625..5118
    Translation

    Target ORF information:

    RefSeq Version XM_021480610.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54a (usp54a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480610.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCATGGA AAAGAAACTA CTTTGCATCT GGCAGCAGTG GGTTACAGGG CATATTCACC 
    CCACGAACCA TGACATCAAT CGCCCCAAGC AAAGGCCTAA TCAACGAGCC GGGACAGAAC
    AGCTGCTTTC TAAACAGCGC TCTCCAGGTT CTCTGGCATC TGGATATCTT CCGACGGAGT
    TTCCGGCAGC TCACCACACA TAAGTGCATG GAGGACTCGT GCATCTTCTG CGCTCTGAAG
    AGCATTTTTG CACAGTTCCA GTTCAGCAGT GAGAAGGTGC TGCCCTCTGA TGCTCTCCGC
    TGCGCTCTGG CCAAAACCTT CCAGGACGAG CAGCGATTCC AGCTCGGCAT TATGGACGAC
    GCGGCTGAGT GCTTCGAAAA TATCCTCATG AGGATCCACT TTCACATCTC TGACGAGACC
    AAGGAGGACA TCTGCACAGC CAAACACTGC ATCCCTCACC AGAAGTTTGC CATGACCCTC
    TTTGAGCAGT GTGTGTGCAG CAACTGTGGA GCCTCCTCAG ACCCTCTGCC CTTCATTCAG
    ATGGTACACT ACATCTCTAC CACCTCTCTG TGTAACCAGG CCGTGCGGAT GCTGGAGTGC
    CGAGAGAAAC CCACTCCAGA CATGTTCGGA GAGCTGCTGA GGAACGCCAG CACCGTGGGA
    GATCTGCGCA ACTGCCCGGG CAACTGTGGA GAGAAGCTGC GTATTCGGCG TGTCCTGATG
    AACTCCCCAG AGATCATCAC CATCGGCCTG GTGTGGGACT CGGACCACTC TGACCTGGCA
    GAAGATGTCA TTCATAGCTT AGGGACTGTC TTGCGGCTCG GAGATCTGTT CTACCGGGTG
    ACAGAGGACA AGGCCAAACA GGCGGAGCTC TACCTGGTGG GCGTGGTCTG TTACTATGGC
    AAACACTACT CCACTTTCTT CTTCCAAACC AAAATCCGCA AGTGGATCTA CTTCGATGAT
    GCTCATGTAA AAGAGATTGG GCCCAAGTGG AAGGATGTGG TGTCTCGCTG TATAAAGGGG
    CATTACCAGC CATTACTGCT GCTCTATGCT GACCCCCGGG GGACGCCAGT GTCCGCTCAG
    GATTTGGCTT CACGTTTAGA CCTACATCAC TACACAAAAG CGTACTATGA CAGTGAGGAT
    TCAGGGCGTG AGCCGTCAAT TTCCAGCGAC ACTCGTACCG ACTCCTCCAC AGACAGCTAT
    AGCTATAAGC ATTCACACTC CCACCACGAG TCTATGGCCT CCCATTTCTC CTCTGACTCC
    CAGGGAACAG TCATCTGTAA TCCTGATAAT GACTCTGCCT CTCACAGCAG CCTGGAAACT
    ACAGGTCCTT TGACTGACAG TGATACAGTG CAGCGTCACA CTCTCAGAAA AGACGGGGTC
    TTGGACAGAA AAGCAGGAGT TTTAGACAGG AAACGAAGTG CCAGCAGGCC ACGAAAATTC
    GAGGCTCGGG GTGATTCAAG GAATCGTGTC TCTAAGACAG ATGAAGTGTT GTCTGCCGGA
    TACCACAGCG AAGGTGAGAC TTTAAAAGAG CAGCAGGTTC CTCGTTCCCT ACCTAAACCA
    AGCTCCAGTC GACTTCGAGA CTTTAAGGAG ACTATGAGTA ACATAATACA CAACAGGCCA
    CTTTCCGCAT CGTCCTCTGG ATCTGTTTGC CCACCCGACT CCACAATTAA AGTCAGAGAC
    TGGGAAGCAG ACAGCACCAG TAGCGAGTCC AAGTCAAGCT CTTCTGGAGG GCGATACCGT
    CCACCGTGGA AGCCTCGGCG TGAGGCACTA AACATCGACA GCATCTTTAG CAGGGAAAGA
    CGCAAGCAGG CAGGGTATAG TCCACTAGGA GCCCTGTTGT CTGAAGACAC CGGACCTCAG
    GTGGGCTTGA TGTCCACAAA GCAGATCACT TTTCAGGACA TACCAGGACC TCTAAGACGA
    GGCTCAGAGC AGCCCCCTCG GCTCATCCAG AGGATGGAGA GTGGCTACGA GAGCAGCGAG
    AGAAACAGCA ACAGCCCTGT CAGTCTGGAC ATGCCTGTTA GCGAGGCCAC CACTAATGGC
    ATGCACAGCT TACAAGAAGG AGCGATGATA AAGCCTCCAC CTGATGTCAA TCCGTCATGG
    AAGACTGTCA CCAAATCCAA AAGCAGCAGT GCCCTATTGC AGGACGTCAA AGCATCAGTC
    AAGGGCAGCA GTCACACGAG TTTAGCAGGT GAGGGCCGCA GTGAACTGGA CGAGCTTCAG
    GAGGAAGTGG CTCGCCGGGC CAGAGAGCAG GAGTTGCAGA GACGAAAGGA AAAGGAGAAA
    GAGGCAGCCA TGGGCTTCAA TCCCAGACCC AGCAAGTTCA TGGATCTGGA TGAGCTCCAG
    AATCAAGGGA AAGGGGAAGG CTTTGAGCGC TGTGTGTTGG AGGCAGAGTC TTTGTTAGAT
    CAGTCTCTGA GGCTGGAACA GGCAGGAGAG GTGGCCGCCG CTCTGTCTGT GGTTAACGAA
    GCAATGTCTA AACTCAGGCA TACCTTGCAT GAGAGCAGCG CTAACGCTCA GGGCAGGATG
    CAAAAATGCA TGAGGAGAGC ACGAGGTCTG CAGCAACGCA TGCAACAGAA GCAACAGGAG
    GAAGAGAAGG AGCAGAAACA GCCCCTCAGT GAACAGTCTG TCCCGGTCCA AATACTTCTG
    ACAAATGTTA AAGAAGAGGA TGAGAAGGCC ATTTCAGAAG ATGCATTAAG ACCACCGTCA
    CCTCTCCACA TAAAACCTCA GTCTACAAGT AAACCGATTT CTGAAACCCC AGGCCCTCCT
    GTCCCCTCTT ACCATACAGA TACATGTTCC CAGAAGGACA ATACAGGGGA CCACCCTAGT
    TCTCTTGATG TGACAGAAAA TGATCGGGGT CCTAGGGACC TTGTAATTAA CACAAACTCT
    CTTGTACAAA CTGCTAGTAA ACGCAGTCCT GCCACTGTGC CTGTGATTGC AGCGAATGGC
    TGTGAGGCTT CCCTGCAGTC CTCCGTTTCG CTCAACACTC CTGCTTATTT CAGTGCCCGT
    CTCACACCTT CCACCACTGA TCACTCTGAA GAAGGTCTCC AGAATCCCCA TCGTCCTCAG
    TCTCTCCATG GCCTGTCCCA AGCACCCCAT GCCTGTCAAA AATCACTCCC TGTTCCCTCA
    CGTAATTGGT CCTGTCGCAG TCTGGAGCGT CCTGATAGTC GTCCCACCAC GCTGGACTCT
    TCCATGTATT CTCCAGTAGA CCTCAGTTCC CCATCGTCCC CCTGTCCTCC ATTCCTGTCT
    TCACCAGTGC TGGAGCAGCG TCCAGCCGGC CCCATGCCTG TGGAACGTTG GGCAGAGAAT
    GTCAATCGAT ACTACAATTC CCAGAAGTGT GCGCGGTCGG GACGTGACTC GCCCTGCGAG
    GAGCTGTCGG AGCTGGACTC ACTCTATCAG GCCAGCTTGA AGGCTCCCAG CATACCTAGA
    GCCACACGGG GAACCAGCCC TCAGCTTGCC AACAGATCAG GTCCTGCTGT TTCTCGTAAA
    CACAGCACTG GATCCTCTGC CACTCTTGGC CGCTCCAAAA CCCCCACAGC AGAGATTGAG
    AGGAGTGCCT ACAGGACGCC AACGTACAGC ACTAAACCAC TTCAGAAGCC TGTTTCTGCT
    GCAGCCTATA ATGAACCGCT GGGTGAGGAT GAATCATACA GTGCTGAGAA CCTGCGACGA
    ATTGCTCGGA GTCTCAGTGG GACTGTTGTT GGAGGACGGA CTGACGTACC CCCATCTCGC
    AGCTTTGACC CTCATGTCTC CAGGATGTCC AAGCATGTGA ATGTGCAACT CCCCACACAT
    CGCTCCAGCA CATCCCTGCA TGTTTTACCA CTTCCCTCCC ACAATCCACC AATGTTCCCT
    CACGAACCAC CGCCCCAACA CCTTCACTCG AGCCACCGTC CTCACCCCGT CCCTACATCA
    TCACACCTGC ACCAGCAGAC ACTGAGGCCC CCTCATGGAC AAACAGGAGA CTGCCAAAAG
    ACCAACAAGT TTCTGGCTGT GCTTGACCGG GGTGAAGCAT TCTCCCGCGA AAACTATCAC
    AACGTCGCAC TGAGCTACGG CACTCTTCCT CGGGCCCCTC GTAGGTCAGT TTCATCTGGG
    CCCCCAGTGT ACCAAATGAG GCCAGGATCC TTACCCCGAA ACCACCCAGA CGCAGCCTAC
    GCCACACTAT CCCGCCCTCA CCGCACTACA GCACCAAGTA GGACGATGGA TGGATTTTAC
    AGGCAACCCC ACCAACACCC TTCATTAACA GCTCCAATCC CGCATCACTC CAGCCGTCAT
    CACCCGCACA CAGAGCCGCC AACTCAACCC CTCCGCCTCG ATGTTCCCCC TGAGAGAGAC
    TGGAGAGCTG CTAGGGACAC TCACGTCTTC CCCAGAACAG ATCCCCGTGG AGGGACGTCC
    CGTGGCCATC CTGTCTATAT CTGCGGGCTG TGCAAACAGA ACCCGGCTGA ACCCACTAGA
    AGCTACTGCC AAGCCTGCAG AGCCCATATG AGCCACTACA GGATGACCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.