usp54 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004915929.3	XP_004915986.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_018095874.2	XP_017951363.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915932.4	XP_004915989.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915934.4	XP_004915991.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915932.3	XP_004915989.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915930.3	XP_004915987.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_012966849.2	XP_012822303.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_018095875.1	XP_017951364.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_004915931.4	XP_004915988.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915930.4	XP_004915987.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915933.3	XP_004915990.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915934.3	XP_004915991.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_031906118.1	XP_031761978.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_018095874.1	XP_017951363.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_012966848.2	XP_012822302.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915931.3	XP_004915988.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_012966850.2	XP_012822304.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
XM_004915929.4	XP_004915986.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_004915933.4	XP_004915990.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_031906117.1	XP_031761977.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_012966848.3	XP_012822302.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_031906119.1	XP_031761979.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45770	XM_018095875.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa62586	XM_004915930.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_031906117.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_004915931.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_004915933.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_018095874.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_004915932.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_012966848.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_004915929.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62586	XM_004915934.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62587	XM_031906118.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62588	XM_031906119.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12828	XM_018095874.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915934.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915932.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915929.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915933.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915930.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_012966848.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12828	XM_004915931.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12830	XM_012966849.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36887	XM_012966850.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

usp54 ( XM_018095875.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951364.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa45770
Clone ID Related Accession (Same CDS sequence)	XM_018095875.1
Accession Version	XM_018095875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GAAAAGAAAA ACCCACCCCT GATATGTTTG GGGAACTGCT GCAAAAAGCC 
AGTACAATGG GAGACTTGAG AAACTGCCCG AGTAATTGTG GTGAAAAAAT CCGAATTCGG 
CGAGTGCTAA TGAATGCCCC TCAGATTATC ACCATTGGGC TGGTGTGGGA CTCAGATCAC 
TCAGATCTAG CCGAAGATGT TATTCACAGT CTTGGAACCT GCCTGAAGCT TGGTGATTTA 
TTTTACAGGG TAACCGATGA CCGAGGAAAA CATTCTGAGC TCTATTTAGT AGGGATGATT 
TGTTACTATG GCAAACATTA CTCTACTTTC TTTTTTCAAA CCAAAATACG CAAGTGGATG 
TATTTTGATG ATGCTCATGT TAAAGAGATT GGTCCAAAAT GGAAAGATGT TGTTACAAAG 
TGTATAAAGG GTCATTATCA ACCATTGCTG CTGCTGTATG CTGATCCCAG GGGTACACCA 
GTAACAGTGC CTGATGTCCT GTCTCAGATG GATCTACGTC AGTACACTAG AACATGCTAT 
GACAGTGAAG ATTCTGGCAG GGAACCATCC ATCTCCAGTG ACACCAGAAC AGATTCTTCT 
ACTGACAGTT ACCATTATAA GCATTCTCAC CATGAGTCAG TGGTGAGCTA CTTCTCCTCT 
GAATCACAAG GCACAGTCAT CTACAATCTG GAAAATGATG CAGCATCTCA AAGCAGCAGA 
GACACAGGAC ACTTGACAGA TAGCGAGTGC AGCCAACGCC ACTTTTCAAG AAAAGGACCC 
TTTACAGACC GAAAACGAAG TTCCAGCCGC CCTCGGAGAA AAGGAGATGA GTCACAGTCT 
TCTGGCTATC ACAGCGAGGG TGAGACACTA AAGGAGAAGC AAGCACCCAG AACAGGCTTG 
AAACTGTCCA GCAGTTCTAG CAGGCTGAGG GATTTTAAAG CTACTGTTAG CAATATTATA 
CATAACAGAC CATCCCAAGC ATCACAGACT TCTTCTCAAG GCTTTCACCA TGTGGGGAGT 
AACCTCGATC AGTCAGAAAT CAAGCCACCT ACAAACTTGT CAATGAGACT GCGGGACTGG 
GATATAGACA ACAGCAACAA TGAAACAAAG TCTGGCTTCT CAAACAGGTA TCGCCCAACC 
TGGCGGCCTA CTCGTGAGCC ATTAAATGTA GACAGTGTCT TCAGGTCAGA AAAGAGGAAA 
AATGCTGCAT ACAGTCCACT TAGTCCTTTT TCAGAAGATT CAGGAAAAGA AATGGCCACT 
AATGAATTCC GAGAAATAGC CAACTCTGAA TCAAAAGACA AGCATCAGTT TAAATACAGA 
AGTTGGGGCA TTGGGAGGGG TGCCCCACAC TTTCTTGACC ACCAGCAGCC TCGTCTTATC 
CAGAGAATGG AGTCTGGGTA TGAAAGCAGT GAGCGGAACA GCAATAGCCC AATCAGCATG 
GACTTGCCAA TATCAGAGAA CTGTAGTGCA TTTAGGGAAT ATCACTCAAG GAAAAGTCCA 
GGTACAGCTC CTACATGGCG AAACATTCCG AAATCTCAGA GCAGCAGTGC ATTAGACATG 
GCAGATTCTG CTTCCAGTGT CTGGGTGAAG GTCCATCCAA CACACATGGC AGTTGAAAGC 
AATGTACCTA TGAAGAGTGA ACTTGATGAA CTACAGGAGG AGGTGGCAAG GAGGGCACGA 
GAACAGGAAC TGCGATGGAA GCGGGAGAAG GAAATTGAGG CTGCAATGGG ATTCAATCCA 
CGACCAAAAC GGTTTTTGGA TTTAGATGAA TTACAGAATC AGGGTAGAAG TGAAAGCTTT 
GAGCGATCTA TGCAAGAAGC AGATTCTGTG TATGAGCAGT CAGTGAGACT GGAACAGAAG 
GGAGATTACA CTTCAGCGCT GGCTTTGTGT AATGAAGCTA TTTCTAAAAT CAGATTTGCC 
ATGCATGATG CCAGATCTAG CACTCACTGC AGAGCCATAG CAGATAAGAA GTTGCAAATG 
TGCATACGAA AAGCACGGAG TCTTCAGGAC CGCATGCAGC TGCCGACAAT TCCTCAGCCG 
CCATCACCAG ATTACCCCTT GCCACAGGGG GAAGCTATAA ACCAATCTAC AAGTGACCAG 
GCTATCTCGG AACAAGTTCC AGTGAACAGA CCGGTAATGC TGGAGACCAA CACAAAGGAG 
GACTACCCAT CCAATCCATT ATTCCACCCG TTAGCTTGTA GCCATGAGTT GCTTCCATTG 
TCCTCTGAGT CTTCCAACTT GTCAGCTTCT GAGCAAAGCC CACATAGCAA AGCGTTTTCT 
AACTTTCTGG CTCCATATAA CAGTCTCGAT TCTGAAGGTC TTGTCTCATC TCCAAAGGAA 
GCAGTGTTTG AGAATAAGGT GAAATCAGAA GCTTATAGCC AAGGCCTAGT AAATGAACAA 
ACAAAAACAG AAAGGGTAAA GAATGTCAGC CCTGATAAAT GTAAGGTGGG AGAGGAAAAT 
GAAGGAAAAG AAGGCCTTCT AACATTGCAT AAGGTGAAAC TAAGTGGGAT TAAATCTGGT 
TCTTTACCAT CATTGCTATT TCCGTGGGCA CGCCGAACAC AGACTCAGTC TCCTGATAGA 
AGCAACCCAC CCAGTCATTG TAATAAATCT GTTAGCACAG ACAAATGTGT TAGTACTGAA 
ACTGGTGAAA GTAATAAACA GATGGGTTCC TGTAATGACA TCACTCCACC TGGTAACACC 
AGCCTCAGCC TTCAACAAGC CAACTTGTGC TCAAGTGCAA ATAGTCATGA GGCATTATTA 
GACAAGCAAG GTGAACTTTG CGGTCCAGAA CCTTGCAAAT CTGAGGCACC AAAGAATAAG 
GGGTTAGTAC GCTCTCTAGC AGAACAGTTT CAAAGGCTCC AGGGGGGCTC ACCAAAACTA 
AACAATTTTG GTGTAAACTG TACTGGTGTT GATATTGGTG AGAAAAAGCC ACTGCCAGAA 
CAGCAATCTT TCAGTCCTTT GGATAATAAA TCCAGTTTGC AGCCCCTTGT AATTCATGAA 
AACAAAGCTA GCTGCACAGA ACAGATAGAC CTCTCACCAG ACTGCCTGGG CAGGTCTTTT 
GACATAAAGG GTTCCAGTAT ACATCTTGTT GATAACCAAA ATAATGGCTC TGAAAAGAAA 
GAAACGCCTG GATGGGTTGT TAATGCTCCA CTTAACCAAG GTAAACTTTT CTATAAAGGA 
GGGAAAAAAG TTAAATTTGC AAGCACTGTC CATTTACCTG GCTCTGTGCC CATGGAGCAG 
TGGGCTGAAG ATGTCACCAG ATATTATAAT GATAAACTGG AGAGTATAGG TGATAAACCT 
AAAGTTGGAC ACACTGTATC TCTTAAACCA AAGTCAATAA GGTATGGCTT ATCAAAAAGT 
TCAATTGATC TGCGCCAGGA GTGGAATCGG GATGAAGAGA TCTCAGACCT GCAAACTTTA 
TATAATGCTA GCCTACAGGC TTGTCCAAAC CGGAATATGC AGTGGAGGTC AGAAAATGCA 
AGACAAGGAT TTGGAAAACC ACTTCTGGAA AACACAGCTG GAAGGAGGGT GCAGACAACT 
GCTGGTAAAG GTCTTTCAAG GACGCCCACA AAAGAAATGG AGCACAATCT TAGTGGAACC 
ACATTTTCAC CTGTGCCAAA GGTCACTCAT GGCGGGGACC AAAGCAGGGA GTCTGAGGAC 
GATGAGCTGT ACAGTGCAGA GAATTTCCGG CGCCTGGCTC GCAGCCTCAG TGGGACAGTC 
ATCAGTGGCA AAGAAGAGGC AGTTGTCTCT TCTCAGAGCT TTGAGGCACC CAATGAGAGG 
AAACCGCTTA TGGACAGCAG CCACTGCTCT CACAGCTCCT CCTTCCACCC TTATTCCCAA 
AATCCTTCTG TCTTATCCTT TGAACCCCAG CACTACCCAA CCCAGCTGCA GCACCCATCC 
TTTGAGGGAC TGGCGCCCAG CATGGTGGGT GCAGCACACA GGCCATCAGG AGGTCCCAAC 
TTCCAAAAAG TTTTGGCTAT GTCTGACAGG GGTGAAAATG TCCAAGGTAA CGAACAGTCG 
AGGATGGCAC TGAGCTATGC AACTTTTCCA AGGGCACCTA GGCTCAGTGC TACACCAACT 
GCAACAGCAT CACAAAGATC TTATGCTGTT CTCGGGCAGC AGACAAACAA TCGAGATCAA 
AATGTTCCAA GTCATTATCG GTGGTCAGGT CATACATCTC AAGGGTCTGC ACCTGACAAA 
CACATGGTTA ATTCCAGTTT CATGACTTCA CCTGGTTGGA GGTCACAAAG TCCTCAAACC 
TCAGGTTATG GAGGCTATTC AAACTCTTCA GCTTATCCTA AAGCTCAAGA TACTGCTGAT 
TACAGTACAC TGAGGATGCC ACGAAGCTCT TCCATAAGTA GAGGAGATAT ATGGGAAACT 
TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC CCGAAGAGAG TAGACATGCC ACCTGAAGAA 
GACTGGAGAC ATAATACTTT TACCACTCAA TTTGGTGGTA GGAAAACACA GCCTGTATTT 
TCACCCCACA GGACCTACTC ATCTGCTGCT GACCTATATG GGGCAAATAG CTGGAGAGCT 
GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951364.1
CDS	105..4688
Translation	MLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_018095875.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095875.1

ORF Insert Sequence:

ATGCTGGAGA GAAAAGAAAA ACCCACCCCT GATATGTTTG GGGAACTGCT GCAAAAAGCC 
AGTACAATGG GAGACTTGAG AAACTGCCCG AGTAATTGTG GTGAAAAAAT CCGAATTCGG 
CGAGTGCTAA TGAATGCCCC TCAGATTATC ACCATTGGGC TGGTGTGGGA CTCAGATCAC 
TCAGATCTAG CCGAAGATGT TATTCACAGT CTTGGAACCT GCCTGAAGCT TGGTGATTTA 
TTTTACAGGG TAACCGATGA CCGAGGAAAA CATTCTGAGC TCTATTTAGT AGGGATGATT 
TGTTACTATG GCAAACATTA CTCTACTTTC TTTTTTCAAA CCAAAATACG CAAGTGGATG 
TATTTTGATG ATGCTCATGT TAAAGAGATT GGTCCAAAAT GGAAAGATGT TGTTACAAAG 
TGTATAAAGG GTCATTATCA ACCATTGCTG CTGCTGTATG CTGATCCCAG GGGTACACCA 
GTAACAGTGC CTGATGTCCT GTCTCAGATG GATCTACGTC AGTACACTAG AACATGCTAT 
GACAGTGAAG ATTCTGGCAG GGAACCATCC ATCTCCAGTG ACACCAGAAC AGATTCTTCT 
ACTGACAGTT ACCATTATAA GCATTCTCAC CATGAGTCAG TGGTGAGCTA CTTCTCCTCT 
GAATCACAAG GCACAGTCAT CTACAATCTG GAAAATGATG CAGCATCTCA AAGCAGCAGA 
GACACAGGAC ACTTGACAGA TAGCGAGTGC AGCCAACGCC ACTTTTCAAG AAAAGGACCC 
TTTACAGACC GAAAACGAAG TTCCAGCCGC CCTCGGAGAA AAGGAGATGA GTCACAGTCT 
TCTGGCTATC ACAGCGAGGG TGAGACACTA AAGGAGAAGC AAGCACCCAG AACAGGCTTG 
AAACTGTCCA GCAGTTCTAG CAGGCTGAGG GATTTTAAAG CTACTGTTAG CAATATTATA 
CATAACAGAC CATCCCAAGC ATCACAGACT TCTTCTCAAG GCTTTCACCA TGTGGGGAGT 
AACCTCGATC AGTCAGAAAT CAAGCCACCT ACAAACTTGT CAATGAGACT GCGGGACTGG 
GATATAGACA ACAGCAACAA TGAAACAAAG TCTGGCTTCT CAAACAGGTA TCGCCCAACC 
TGGCGGCCTA CTCGTGAGCC ATTAAATGTA GACAGTGTCT TCAGGTCAGA AAAGAGGAAA 
AATGCTGCAT ACAGTCCACT TAGTCCTTTT TCAGAAGATT CAGGAAAAGA AATGGCCACT 
AATGAATTCC GAGAAATAGC CAACTCTGAA TCAAAAGACA AGCATCAGTT TAAATACAGA 
AGTTGGGGCA TTGGGAGGGG TGCCCCACAC TTTCTTGACC ACCAGCAGCC TCGTCTTATC 
CAGAGAATGG AGTCTGGGTA TGAAAGCAGT GAGCGGAACA GCAATAGCCC AATCAGCATG 
GACTTGCCAA TATCAGAGAA CTGTAGTGCA TTTAGGGAAT ATCACTCAAG GAAAAGTCCA 
GGTACAGCTC CTACATGGCG AAACATTCCG AAATCTCAGA GCAGCAGTGC ATTAGACATG 
GCAGATTCTG CTTCCAGTGT CTGGGTGAAG GTCCATCCAA CACACATGGC AGTTGAAAGC 
AATGTACCTA TGAAGAGTGA ACTTGATGAA CTACAGGAGG AGGTGGCAAG GAGGGCACGA 
GAACAGGAAC TGCGATGGAA GCGGGAGAAG GAAATTGAGG CTGCAATGGG ATTCAATCCA 
CGACCAAAAC GGTTTTTGGA TTTAGATGAA TTACAGAATC AGGGTAGAAG TGAAAGCTTT 
GAGCGATCTA TGCAAGAAGC AGATTCTGTG TATGAGCAGT CAGTGAGACT GGAACAGAAG 
GGAGATTACA CTTCAGCGCT GGCTTTGTGT AATGAAGCTA TTTCTAAAAT CAGATTTGCC 
ATGCATGATG CCAGATCTAG CACTCACTGC AGAGCCATAG CAGATAAGAA GTTGCAAATG 
TGCATACGAA AAGCACGGAG TCTTCAGGAC CGCATGCAGC TGCCGACAAT TCCTCAGCCG 
CCATCACCAG ATTACCCCTT GCCACAGGGG GAAGCTATAA ACCAATCTAC AAGTGACCAG 
GCTATCTCGG AACAAGTTCC AGTGAACAGA CCGGTAATGC TGGAGACCAA CACAAAGGAG 
GACTACCCAT CCAATCCATT ATTCCACCCG TTAGCTTGTA GCCATGAGTT GCTTCCATTG 
TCCTCTGAGT CTTCCAACTT GTCAGCTTCT GAGCAAAGCC CACATAGCAA AGCGTTTTCT 
AACTTTCTGG CTCCATATAA CAGTCTCGAT TCTGAAGGTC TTGTCTCATC TCCAAAGGAA 
GCAGTGTTTG AGAATAAGGT GAAATCAGAA GCTTATAGCC AAGGCCTAGT AAATGAACAA 
ACAAAAACAG AAAGGGTAAA GAATGTCAGC CCTGATAAAT GTAAGGTGGG AGAGGAAAAT 
GAAGGAAAAG AAGGCCTTCT AACATTGCAT AAGGTGAAAC TAAGTGGGAT TAAATCTGGT 
TCTTTACCAT CATTGCTATT TCCGTGGGCA CGCCGAACAC AGACTCAGTC TCCTGATAGA 
AGCAACCCAC CCAGTCATTG TAATAAATCT GTTAGCACAG ACAAATGTGT TAGTACTGAA 
ACTGGTGAAA GTAATAAACA GATGGGTTCC TGTAATGACA TCACTCCACC TGGTAACACC 
AGCCTCAGCC TTCAACAAGC CAACTTGTGC TCAAGTGCAA ATAGTCATGA GGCATTATTA 
GACAAGCAAG GTGAACTTTG CGGTCCAGAA CCTTGCAAAT CTGAGGCACC AAAGAATAAG 
GGGTTAGTAC GCTCTCTAGC AGAACAGTTT CAAAGGCTCC AGGGGGGCTC ACCAAAACTA 
AACAATTTTG GTGTAAACTG TACTGGTGTT GATATTGGTG AGAAAAAGCC ACTGCCAGAA 
CAGCAATCTT TCAGTCCTTT GGATAATAAA TCCAGTTTGC AGCCCCTTGT AATTCATGAA 
AACAAAGCTA GCTGCACAGA ACAGATAGAC CTCTCACCAG ACTGCCTGGG CAGGTCTTTT 
GACATAAAGG GTTCCAGTAT ACATCTTGTT GATAACCAAA ATAATGGCTC TGAAAAGAAA 
GAAACGCCTG GATGGGTTGT TAATGCTCCA CTTAACCAAG GTAAACTTTT CTATAAAGGA 
GGGAAAAAAG TTAAATTTGC AAGCACTGTC CATTTACCTG GCTCTGTGCC CATGGAGCAG 
TGGGCTGAAG ATGTCACCAG ATATTATAAT GATAAACTGG AGAGTATAGG TGATAAACCT 
AAAGTTGGAC ACACTGTATC TCTTAAACCA AAGTCAATAA GGTATGGCTT ATCAAAAAGT 
TCAATTGATC TGCGCCAGGA GTGGAATCGG GATGAAGAGA TCTCAGACCT GCAAACTTTA 
TATAATGCTA GCCTACAGGC TTGTCCAAAC CGGAATATGC AGTGGAGGTC AGAAAATGCA 
AGACAAGGAT TTGGAAAACC ACTTCTGGAA AACACAGCTG GAAGGAGGGT GCAGACAACT 
GCTGGTAAAG GTCTTTCAAG GACGCCCACA AAAGAAATGG AGCACAATCT TAGTGGAACC 
ACATTTTCAC CTGTGCCAAA GGTCACTCAT GGCGGGGACC AAAGCAGGGA GTCTGAGGAC 
GATGAGCTGT ACAGTGCAGA GAATTTCCGG CGCCTGGCTC GCAGCCTCAG TGGGACAGTC 
ATCAGTGGCA AAGAAGAGGC AGTTGTCTCT TCTCAGAGCT TTGAGGCACC CAATGAGAGG 
AAACCGCTTA TGGACAGCAG CCACTGCTCT CACAGCTCCT CCTTCCACCC TTATTCCCAA 
AATCCTTCTG TCTTATCCTT TGAACCCCAG CACTACCCAA CCCAGCTGCA GCACCCATCC 
TTTGAGGGAC TGGCGCCCAG CATGGTGGGT GCAGCACACA GGCCATCAGG AGGTCCCAAC 
TTCCAAAAAG TTTTGGCTAT GTCTGACAGG GGTGAAAATG TCCAAGGTAA CGAACAGTCG 
AGGATGGCAC TGAGCTATGC AACTTTTCCA AGGGCACCTA GGCTCAGTGC TACACCAACT 
GCAACAGCAT CACAAAGATC TTATGCTGTT CTCGGGCAGC AGACAAACAA TCGAGATCAA 
AATGTTCCAA GTCATTATCG GTGGTCAGGT CATACATCTC AAGGGTCTGC ACCTGACAAA 
CACATGGTTA ATTCCAGTTT CATGACTTCA CCTGGTTGGA GGTCACAAAG TCCTCAAACC 
TCAGGTTATG GAGGCTATTC AAACTCTTCA GCTTATCCTA AAGCTCAAGA TACTGCTGAT 
TACAGTACAC TGAGGATGCC ACGAAGCTCT TCCATAAGTA GAGGAGATAT ATGGGAAACT 
TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC CCGAAGAGAG TAGACATGCC ACCTGAAGAA 
GACTGGAGAC ATAATACTTT TACCACTCAA TTTGGTGGTA GGAAAACACA GCCTGTATTT 
TCACCCCACA GGACCTACTC ATCTGCTGCT GACCTATATG GGGCAAATAG CTGGAGAGCT 
GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_018095874.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951363.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_018095874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951363.1
CDS	1425..6599
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_018095874.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095874.2

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915932.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915989.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915989.1
CDS	1369..6543
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915932.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915932.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915934.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915991.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915934.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915934.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915991.1
CDS	1215..6389
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915934.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915934.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915931.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915988.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915988.1
CDS	1622..6796
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915931.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915931.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915930.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915987.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915930.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915930.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915987.1
CDS	1706..6880
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915930.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915930.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915929.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915986.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915929.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915929.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915986.1
CDS	1802..6976
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915929.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915929.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915933.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915990.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_004915933.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915933.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915990.1
CDS	1295..6469
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915933.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915933.4

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_031906117.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761977.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_031906117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761977.1
CDS	1718..6892
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_031906117.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906117.1

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_012966848.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822302.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.

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CloneID	OXa62586
Clone ID Related Accession (Same CDS sequence)	XM_018095874.2 , XM_004915932.4 , XM_004915934.4 , XM_004915931.4 , XM_004915930.4 , XM_004915929.4 , XM_004915933.4 , XM_031906117.1 , XM_012966848.3
Accession Version	XM_012966848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822302.1
CDS	1245..6419
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_012966848.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966848.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_031906118.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761978.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa62587
Clone ID Related Accession (Same CDS sequence)	XM_031906118.1
Accession Version	XM_031906118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTTG CTGATGCAAC CTCTCTTGAC CAGCAGGTGT TATGGCATCT AGACATATTC 
CGCCGGAGCT TCCGACAACT TACAACACAC AAGTGTATGG GAGACTCCTG TATATTCTGT 
GCACTGAAGG GAATCTTCAA CCAGTTTCAG TGTAGCAGTG AGAAGGTGTT ACCATCAGAT 
ACTCTTCGTT GTGCTCTTGC TAAAACATTT CAGGATGAGC AGCGGTTCCA GTTGGGCATC 
ATGGATGATG CAGCAGAATG CTTTGAAAAC TTATTAATTC GCCTTCACAT CCACATCGCA 
GATGAGTCCA AAGAAGATAT CTGTACTGCC CAGCATTGCA TCTCCCACCA GAAGTTTGCT 
ATGACCTTGT TTGAGCAGTG TGTTTGTACA AGCTGTGGTG CCACTTCTGA CCCCCTGCCT 
TTTATACAGA TGGTGCATTA TATATCTACT ACTGCACTCT GCAATCAGGC AATCTTTATG 
CTGGAGAGAA AAGAAAAACC CACCCCTGAT ATGTTTGGGG AACTGCTGCA AAAAGCCAGT 
ACAATGGGAG ACTTGAGAAA CTGCCCGAGT AATTGTGGTG AAAAAATCCG AATTCGGCGA 
GTGCTAATGA ATGCCCCTCA GATTATCACC ATTGGGCTGG TGTGGGACTC AGATCACTCA 
GATCTAGCCG AAGATGTTAT TCACAGTCTT GGAACCTGCC TGAAGCTTGG TGATTTATTT 
TACAGGGTAA CCGATGACCG AGGAAAACAT TCTGAGCTCT ATTTAGTAGG GATGATTTGT 
TACTATGGCA AACATTACTC TACTTTCTTT TTTCAAACCA AAATACGCAA GTGGATGTAT 
TTTGATGATG CTCATGTTAA AGAGATTGGT CCAAAATGGA AAGATGTTGT TACAAAGTGT 
ATAAAGGGTC ATTATCAACC ATTGCTGCTG CTGTATGCTG ATCCCAGGGG TACACCAGTA 
ACAGTGCCTG ATGTCCTGTC TCAGATGGAT CTACGTCAGT ACACTAGAAC ATGCTATGAC 
AGTGAAGATT CTGGCAGGGA ACCATCCATC TCCAGTGACA CCAGAACAGA TTCTTCTACT 
GACAGTTACC ATTATAAGCA TTCTCACCAT GAGTCAGTGG TGAGCTACTT CTCCTCTGAA 
TCACAAGGCA CAGTCATCTA CAATCTGGAA AATGATGCAG CATCTCAAAG CAGCAGAGAC 
ACAGGACACT TGACAGATAG CGAGTGCAGC CAACGCCACT TTTCAAGAAA AGGACCCTTT 
ACAGACCGAA AACGAAGTTC CAGCCGCCCT CGGAGAAAAG GAGATGAGTC ACAGTCTTCT 
GGCTATCACA GCGAGGGTGA GACACTAAAG GAGAAGCAAG CACCCAGAAC AGGCTTGAAA 
CTGTCCAGCA GTTCTAGCAG GCTGAGGGAT TTTAAAGCTA CTGTTAGCAA TATTATACAT 
AACAGACCAT CCCAAGCATC ACAGACTTCT TCTCAAGGCT TTCACCATGT GGGGAGTAAC 
CTCGATCAGT CAGAAATCAA GCCACCTACA AACTTGTCAA TGAGACTGCG GGACTGGGAT 
ATAGACAACA GCAACAATGA AACAAAGTCT GGCTTCTCAA ACAGGTATCG CCCAACCTGG 
CGGCCTACTC GTGAGCCATT AAATGTAGAC AGTGTCTTCA GGTCAGAAAA GAGGAAAAAT 
GCTGCATACA GTCCACTTAG TCCTTTTTCA GAAGATTCAG GAAAAGAAAT GGCCACTAAT 
GAATTCCGAG AAATAGCCAA CTCTGAATCA AAAGACAAGC ATCAGTTTAA ATACAGAAGT 
TGGGGCATTG GGAGGGGTGC CCCACACTTT CTTGACCACC AGCAGCCTCG TCTTATCCAG 
AGAATGGAGT CTGGGTATGA AAGCAGTGAG CGGAACAGCA ATAGCCCAAT CAGCATGGAC 
TTGCCAATAT CAGAGAACTG TAGTGCATTT AGGGAATATC ACTCAAGGAA AAGTCCAGGT 
ACAGCTCCTA CATGGCGAAA CATTCCGAAA TCTCAGAGCA GCAGTGCATT AGACATGGCA 
GATTCTGCTT CCAGTGTCTG GGTGAAGGTC CATCCAACAC ACATGGCAGT TGAAAGCAAT 
GTACCTATGA AGAGTGAACT TGATGAACTA CAGGAGGAGG TGGCAAGGAG GGCACGAGAA 
CAGGAACTGC GATGGAAGCG GGAGAAGGAA ATTGAGGCTG CAATGGGATT CAATCCACGA 
CCAAAACGGT TTTTGGATTT AGATGAATTA CAGAATCAGG GTAGAAGTGA AAGCTTTGAG 
CGATCTATGC AAGAAGCAGA TTCTGTGTAT GAGCAGTCAG TGAGACTGGA ACAGAAGGGA 
GATTACACTT CAGCGCTGGC TTTGTGTAAT GAAGCTATTT CTAAAATCAG ATTTGCCATG 
CATGATGCCA GATCTAGCAC TCACTGCAGA GCCATAGCAG ATAAGAAGTT GCAAATGTGC 
ATACGAAAAG CACGGAGTCT TCAGGACCGC ATGCAGCTGC CGACAATTCC TCAGCCGCCA 
TCACCAGATT ACCCCTTGCC ACAGGGGGAA GCTATAAACC AATCTACAAG TGACCAGGCT 
ATCTCGGAAC AAGTTCCAGT GAACAGACCG GTAATGCTGG AGACCAACAC AAAGGAGGAC 
TACCCATCCA ATCCATTATT CCACCCGTTA GCTTGTAGCC ATGAGTTGCT TCCATTGTCC 
TCTGAGTCTT CCAACTTGTC AGCTTCTGAG CAAAGCCCAC ATAGCAAAGC GTTTTCTAAC 
TTTCTGGCTC CATATAACAG TCTCGATTCT GAAGGTCTTG TCTCATCTCC AAAGGAAGCA 
GTGTTTGAGA ATAAGGTGAA ATCAGAAGCT TATAGCCAAG GCCTAGTAAA TGAACAAACA 
AAAACAGAAA GGGTAAAGAA TGTCAGCCCT GATAAATGTA AGGTGGGAGA GGAAAATGAA 
GGAAAAGAAG GCCTTCTAAC ATTGCATAAG GTGAAACTAA GTGGGATTAA ATCTGGTTCT 
TTACCATCAT TGCTATTTCC GTGGGCACGC CGAACACAGA CTCAGTCTCC TGATAGAAGC 
AACCCACCCA GTCATTGTAA TAAATCTGTT AGCACAGACA AATGTGTTAG TACTGAAACT 
GGTGAAAGTA ATAAACAGAT GGGTTCCTGT AATGACATCA CTCCACCTGG TAACACCAGC 
CTCAGCCTTC AACAAGCCAA CTTGTGCTCA AGTGCAAATA GTCATGAGGC ATTATTAGAC 
AAGCAAGGTG AACTTTGCGG TCCAGAACCT TGCAAATCTG AGGCACCAAA GAATAAGGGG 
TTAGTACGCT CTCTAGCAGA ACAGTTTCAA AGGCTCCAGG GGGGCTCACC AAAACTAAAC 
AATTTTGGTG TAAACTGTAC TGGTGTTGAT ATTGGTGAGA AAAAGCCACT GCCAGAACAG 
CAATCTTTCA GTCCTTTGGA TAATAAATCC AGTTTGCAGC CCCTTGTAAT TCATGAAAAC 
AAAGCTAGCT GCACAGAACA GATAGACCTC TCACCAGACT GCCTGGGCAG GTCTTTTGAC 
ATAAAGGGTT CCAGTATACA TCTTGTTGAT AACCAAAATA ATGGCTCTGA AAAGAAAGAA 
ACGCCTGGAT GGGTTGTTAA TGCTCCACTT AACCAAGGTA AACTTTTCTA TAAAGGAGGG 
AAAAAAGTTA AATTTGCAAG CACTGTCCAT TTACCTGGCT CTGTGCCCAT GGAGCAGTGG 
GCTGAAGATG TCACCAGATA TTATAATGAT AAACTGGAGA GTATAGGTGA TAAACCTAAA 
GTTGGACACA CTGTATCTCT TAAACCAAAG TCAATAAGGT ATGGCTTATC AAAAAGTTCA 
ATTGATCTGC GCCAGGAGTG GAATCGGGAT GAAGAGATCT CAGACCTGCA AACTTTATAT 
AATGCTAGCC TACAGGCTTG TCCAAACCGG AATATGCAGT GGAGGTCAGA AAATGCAAGA 
CAAGGATTTG GAAAACCACT TCTGGAAAAC ACAGCTGGAA GGAGGGTGCA GACAACTGCT 
GGTAAAGGTC TTTCAAGGAC GCCCACAAAA GAAATGGAGC ACAATCTTAG TGGAACCACA 
TTTTCACCTG TGCCAAAGGT CACTCATGGC GGGGACCAAA GCAGGGAGTC TGAGGACGAT 
GAGCTGTACA GTGCAGAGAA TTTCCGGCGC CTGGCTCGCA GCCTCAGTGG GACAGTCATC 
AGTGGCAAAG AAGAGGCAGT TGTCTCTTCT CAGAGCTTTG AGGCACCCAA TGAGAGGAAA 
CCGCTTATGG ACAGCAGCCA CTGCTCTCAC AGCTCCTCCT TCCACCCTTA TTCCCAAAAT 
CCTTCTGTCT TATCCTTTGA ACCCCAGCAC TACCCAACCC AGCTGCAGCA CCCATCCTTT 
GAGGGACTGG CGCCCAGCAT GGTGGGTGCA GCACACAGGC CATCAGGAGG TCCCAACTTC 
CAAAAAGTTT TGGCTATGTC TGACAGGGGT GAAAATGTCC AAGGTAACGA ACAGTCGAGG 
ATGGCACTGA GCTATGCAAC TTTTCCAAGG GCACCTAGGC TCAGTGCTAC ACCAACTGCA 
ACAGCATCAC AAAGATCTTA TGCTGTTCTC GGGCAGCAGA CAAACAATCG AGATCAAAAT 
GTTCCAAGTC ATTATCGGTG GTCAGGTCAT ACATCTCAAG GGTCTGCACC TGACAAACAC 
ATGGTTAATT CCAGTTTCAT GACTTCACCT GGTTGGAGGT CACAAAGTCC TCAAACCTCA 
GGTTATGGAG GCTATTCAAA CTCTTCAGCT TATCCTAAAG CTCAAGATAC TGCTGATTAC 
AGTACACTGA GGATGCCACG AAGCTCTTCC ATAAGTAGAG GAGATATATG GGAAACTTCT 
CAGGCTAATC AGTCAAGAGT TCAAGGCCCG AAGAGAGTAG ACATGCCACC TGAAGAAGAC 
TGGAGACATA ATACTTTTAC CACTCAATTT GGTGGTAGGA AAACACAGCC TGTATTTTCA 
CCCCACAGGA CCTACTCATC TGCTGCTGAC CTATATGGGG CAAATAGCTG GAGAGCTGCC 
TTGAACAGAA ATGGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761978.1
CDS	223..5283
Translation	MHFADATSLDQQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_031906118.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906118.1

ORF Insert Sequence:

ATGCATTTTG CTGATGCAAC CTCTCTTGAC CAGCAGGTGT TATGGCATCT AGACATATTC 
CGCCGGAGCT TCCGACAACT TACAACACAC AAGTGTATGG GAGACTCCTG TATATTCTGT 
GCACTGAAGG GAATCTTCAA CCAGTTTCAG TGTAGCAGTG AGAAGGTGTT ACCATCAGAT 
ACTCTTCGTT GTGCTCTTGC TAAAACATTT CAGGATGAGC AGCGGTTCCA GTTGGGCATC 
ATGGATGATG CAGCAGAATG CTTTGAAAAC TTATTAATTC GCCTTCACAT CCACATCGCA 
GATGAGTCCA AAGAAGATAT CTGTACTGCC CAGCATTGCA TCTCCCACCA GAAGTTTGCT 
ATGACCTTGT TTGAGCAGTG TGTTTGTACA AGCTGTGGTG CCACTTCTGA CCCCCTGCCT 
TTTATACAGA TGGTGCATTA TATATCTACT ACTGCACTCT GCAATCAGGC AATCTTTATG 
CTGGAGAGAA AAGAAAAACC CACCCCTGAT ATGTTTGGGG AACTGCTGCA AAAAGCCAGT 
ACAATGGGAG ACTTGAGAAA CTGCCCGAGT AATTGTGGTG AAAAAATCCG AATTCGGCGA 
GTGCTAATGA ATGCCCCTCA GATTATCACC ATTGGGCTGG TGTGGGACTC AGATCACTCA 
GATCTAGCCG AAGATGTTAT TCACAGTCTT GGAACCTGCC TGAAGCTTGG TGATTTATTT 
TACAGGGTAA CCGATGACCG AGGAAAACAT TCTGAGCTCT ATTTAGTAGG GATGATTTGT 
TACTATGGCA AACATTACTC TACTTTCTTT TTTCAAACCA AAATACGCAA GTGGATGTAT 
TTTGATGATG CTCATGTTAA AGAGATTGGT CCAAAATGGA AAGATGTTGT TACAAAGTGT 
ATAAAGGGTC ATTATCAACC ATTGCTGCTG CTGTATGCTG ATCCCAGGGG TACACCAGTA 
ACAGTGCCTG ATGTCCTGTC TCAGATGGAT CTACGTCAGT ACACTAGAAC ATGCTATGAC 
AGTGAAGATT CTGGCAGGGA ACCATCCATC TCCAGTGACA CCAGAACAGA TTCTTCTACT 
GACAGTTACC ATTATAAGCA TTCTCACCAT GAGTCAGTGG TGAGCTACTT CTCCTCTGAA 
TCACAAGGCA CAGTCATCTA CAATCTGGAA AATGATGCAG CATCTCAAAG CAGCAGAGAC 
ACAGGACACT TGACAGATAG CGAGTGCAGC CAACGCCACT TTTCAAGAAA AGGACCCTTT 
ACAGACCGAA AACGAAGTTC CAGCCGCCCT CGGAGAAAAG GAGATGAGTC ACAGTCTTCT 
GGCTATCACA GCGAGGGTGA GACACTAAAG GAGAAGCAAG CACCCAGAAC AGGCTTGAAA 
CTGTCCAGCA GTTCTAGCAG GCTGAGGGAT TTTAAAGCTA CTGTTAGCAA TATTATACAT 
AACAGACCAT CCCAAGCATC ACAGACTTCT TCTCAAGGCT TTCACCATGT GGGGAGTAAC 
CTCGATCAGT CAGAAATCAA GCCACCTACA AACTTGTCAA TGAGACTGCG GGACTGGGAT 
ATAGACAACA GCAACAATGA AACAAAGTCT GGCTTCTCAA ACAGGTATCG CCCAACCTGG 
CGGCCTACTC GTGAGCCATT AAATGTAGAC AGTGTCTTCA GGTCAGAAAA GAGGAAAAAT 
GCTGCATACA GTCCACTTAG TCCTTTTTCA GAAGATTCAG GAAAAGAAAT GGCCACTAAT 
GAATTCCGAG AAATAGCCAA CTCTGAATCA AAAGACAAGC ATCAGTTTAA ATACAGAAGT 
TGGGGCATTG GGAGGGGTGC CCCACACTTT CTTGACCACC AGCAGCCTCG TCTTATCCAG 
AGAATGGAGT CTGGGTATGA AAGCAGTGAG CGGAACAGCA ATAGCCCAAT CAGCATGGAC 
TTGCCAATAT CAGAGAACTG TAGTGCATTT AGGGAATATC ACTCAAGGAA AAGTCCAGGT 
ACAGCTCCTA CATGGCGAAA CATTCCGAAA TCTCAGAGCA GCAGTGCATT AGACATGGCA 
GATTCTGCTT CCAGTGTCTG GGTGAAGGTC CATCCAACAC ACATGGCAGT TGAAAGCAAT 
GTACCTATGA AGAGTGAACT TGATGAACTA CAGGAGGAGG TGGCAAGGAG GGCACGAGAA 
CAGGAACTGC GATGGAAGCG GGAGAAGGAA ATTGAGGCTG CAATGGGATT CAATCCACGA 
CCAAAACGGT TTTTGGATTT AGATGAATTA CAGAATCAGG GTAGAAGTGA AAGCTTTGAG 
CGATCTATGC AAGAAGCAGA TTCTGTGTAT GAGCAGTCAG TGAGACTGGA ACAGAAGGGA 
GATTACACTT CAGCGCTGGC TTTGTGTAAT GAAGCTATTT CTAAAATCAG ATTTGCCATG 
CATGATGCCA GATCTAGCAC TCACTGCAGA GCCATAGCAG ATAAGAAGTT GCAAATGTGC 
ATACGAAAAG CACGGAGTCT TCAGGACCGC ATGCAGCTGC CGACAATTCC TCAGCCGCCA 
TCACCAGATT ACCCCTTGCC ACAGGGGGAA GCTATAAACC AATCTACAAG TGACCAGGCT 
ATCTCGGAAC AAGTTCCAGT GAACAGACCG GTAATGCTGG AGACCAACAC AAAGGAGGAC 
TACCCATCCA ATCCATTATT CCACCCGTTA GCTTGTAGCC ATGAGTTGCT TCCATTGTCC 
TCTGAGTCTT CCAACTTGTC AGCTTCTGAG CAAAGCCCAC ATAGCAAAGC GTTTTCTAAC 
TTTCTGGCTC CATATAACAG TCTCGATTCT GAAGGTCTTG TCTCATCTCC AAAGGAAGCA 
GTGTTTGAGA ATAAGGTGAA ATCAGAAGCT TATAGCCAAG GCCTAGTAAA TGAACAAACA 
AAAACAGAAA GGGTAAAGAA TGTCAGCCCT GATAAATGTA AGGTGGGAGA GGAAAATGAA 
GGAAAAGAAG GCCTTCTAAC ATTGCATAAG GTGAAACTAA GTGGGATTAA ATCTGGTTCT 
TTACCATCAT TGCTATTTCC GTGGGCACGC CGAACACAGA CTCAGTCTCC TGATAGAAGC 
AACCCACCCA GTCATTGTAA TAAATCTGTT AGCACAGACA AATGTGTTAG TACTGAAACT 
GGTGAAAGTA ATAAACAGAT GGGTTCCTGT AATGACATCA CTCCACCTGG TAACACCAGC 
CTCAGCCTTC AACAAGCCAA CTTGTGCTCA AGTGCAAATA GTCATGAGGC ATTATTAGAC 
AAGCAAGGTG AACTTTGCGG TCCAGAACCT TGCAAATCTG AGGCACCAAA GAATAAGGGG 
TTAGTACGCT CTCTAGCAGA ACAGTTTCAA AGGCTCCAGG GGGGCTCACC AAAACTAAAC 
AATTTTGGTG TAAACTGTAC TGGTGTTGAT ATTGGTGAGA AAAAGCCACT GCCAGAACAG 
CAATCTTTCA GTCCTTTGGA TAATAAATCC AGTTTGCAGC CCCTTGTAAT TCATGAAAAC 
AAAGCTAGCT GCACAGAACA GATAGACCTC TCACCAGACT GCCTGGGCAG GTCTTTTGAC 
ATAAAGGGTT CCAGTATACA TCTTGTTGAT AACCAAAATA ATGGCTCTGA AAAGAAAGAA 
ACGCCTGGAT GGGTTGTTAA TGCTCCACTT AACCAAGGTA AACTTTTCTA TAAAGGAGGG 
AAAAAAGTTA AATTTGCAAG CACTGTCCAT TTACCTGGCT CTGTGCCCAT GGAGCAGTGG 
GCTGAAGATG TCACCAGATA TTATAATGAT AAACTGGAGA GTATAGGTGA TAAACCTAAA 
GTTGGACACA CTGTATCTCT TAAACCAAAG TCAATAAGGT ATGGCTTATC AAAAAGTTCA 
ATTGATCTGC GCCAGGAGTG GAATCGGGAT GAAGAGATCT CAGACCTGCA AACTTTATAT 
AATGCTAGCC TACAGGCTTG TCCAAACCGG AATATGCAGT GGAGGTCAGA AAATGCAAGA 
CAAGGATTTG GAAAACCACT TCTGGAAAAC ACAGCTGGAA GGAGGGTGCA GACAACTGCT 
GGTAAAGGTC TTTCAAGGAC GCCCACAAAA GAAATGGAGC ACAATCTTAG TGGAACCACA 
TTTTCACCTG TGCCAAAGGT CACTCATGGC GGGGACCAAA GCAGGGAGTC TGAGGACGAT 
GAGCTGTACA GTGCAGAGAA TTTCCGGCGC CTGGCTCGCA GCCTCAGTGG GACAGTCATC 
AGTGGCAAAG AAGAGGCAGT TGTCTCTTCT CAGAGCTTTG AGGCACCCAA TGAGAGGAAA 
CCGCTTATGG ACAGCAGCCA CTGCTCTCAC AGCTCCTCCT TCCACCCTTA TTCCCAAAAT 
CCTTCTGTCT TATCCTTTGA ACCCCAGCAC TACCCAACCC AGCTGCAGCA CCCATCCTTT 
GAGGGACTGG CGCCCAGCAT GGTGGGTGCA GCACACAGGC CATCAGGAGG TCCCAACTTC 
CAAAAAGTTT TGGCTATGTC TGACAGGGGT GAAAATGTCC AAGGTAACGA ACAGTCGAGG 
ATGGCACTGA GCTATGCAAC TTTTCCAAGG GCACCTAGGC TCAGTGCTAC ACCAACTGCA 
ACAGCATCAC AAAGATCTTA TGCTGTTCTC GGGCAGCAGA CAAACAATCG AGATCAAAAT 
GTTCCAAGTC ATTATCGGTG GTCAGGTCAT ACATCTCAAG GGTCTGCACC TGACAAACAC 
ATGGTTAATT CCAGTTTCAT GACTTCACCT GGTTGGAGGT CACAAAGTCC TCAAACCTCA 
GGTTATGGAG GCTATTCAAA CTCTTCAGCT TATCCTAAAG CTCAAGATAC TGCTGATTAC 
AGTACACTGA GGATGCCACG AAGCTCTTCC ATAAGTAGAG GAGATATATG GGAAACTTCT 
CAGGCTAATC AGTCAAGAGT TCAAGGCCCG AAGAGAGTAG ACATGCCACC TGAAGAAGAC 
TGGAGACATA ATACTTTTAC CACTCAATTT GGTGGTAGGA AAACACAGCC TGTATTTTCA 
CCCCACAGGA CCTACTCATC TGCTGCTGAC CTATATGGGG CAAATAGCTG GAGAGCTGCC 
TTGAACAGAA ATGGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_031906119.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761979.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.

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CloneID	OXa62588
Clone ID Related Accession (Same CDS sequence)	XM_031906119.1
Accession Version	XM_031906119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGAGGCACC CAATGAGAGG AAACCGCTTA TGGACAGCAG CCACTGCTCT 
CACAGCTCCT CCTTCCACCC TTATTCCCAA AATCCTTCTG TCTTATCCTT TGAACCCCAG 
CACTACCCAA CCCAGCTGCA GCACCCATCC TTTGAGGGAC TGGCGCCCAG CATGGTGGGT 
GCAGCACACA GGCCATCAGG AGGTCCCAAC TTCCAAAAAG TTTTGGCTAT GTCTGACAGG 
GGTGAAAATG TCCAAGGTAA CGAACAGTCG AGGATGGCAC TGAGCTATGC AACTTTTCCA 
AGGGCACCTA GGCTCAGTGC TACACCAACT GCAACAGCAT CACAAAGATC TTATGCTGTT 
CTCGGGCAGC AGACAAACAA TCGAGATCAA AATGTTCCAA GTCATTATCG GTGGTCAGGT 
CATACATCTC AAGGGTCTGC ACCTGACAAA CACATGGTTA ATTCCAGTTT CATGACTTCA 
CCTGGTTGGA GGTCACAAAG TCCTCAAACC TCAGGTTATG GAGGCTATTC AAACTCTTCA 
GCTTATCCTA AAGCTCAAGA TACTGCTGAT TACAGTACAC TGAGGATGCC ACGAAGCTCT 
TCCATAAGTA GAGGAGATAT ATGGGAAACT TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC 
CCGAAGAGAG TAGACATGCC ACCTGAAGAA GACTGGAGAC ATAATACTTT TACCACTCAA 
TTTGGTGGTA GGAAAACACA GCCTGTATTT TCACCCCACA GGACCTACTC ATCTGCTGCT 
GACCTATATG GGGCAAATAG CTGGAGAGCT GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761979.1
CDS	1808..6841
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_031906119.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906119.1

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCTATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGAGGCACC CAATGAGAGG AAACCGCTTA TGGACAGCAG CCACTGCTCT 
CACAGCTCCT CCTTCCACCC TTATTCCCAA AATCCTTCTG TCTTATCCTT TGAACCCCAG 
CACTACCCAA CCCAGCTGCA GCACCCATCC TTTGAGGGAC TGGCGCCCAG CATGGTGGGT 
GCAGCACACA GGCCATCAGG AGGTCCCAAC TTCCAAAAAG TTTTGGCTAT GTCTGACAGG 
GGTGAAAATG TCCAAGGTAA CGAACAGTCG AGGATGGCAC TGAGCTATGC AACTTTTCCA 
AGGGCACCTA GGCTCAGTGC TACACCAACT GCAACAGCAT CACAAAGATC TTATGCTGTT 
CTCGGGCAGC AGACAAACAA TCGAGATCAA AATGTTCCAA GTCATTATCG GTGGTCAGGT 
CATACATCTC AAGGGTCTGC ACCTGACAAA CACATGGTTA ATTCCAGTTT CATGACTTCA 
CCTGGTTGGA GGTCACAAAG TCCTCAAACC TCAGGTTATG GAGGCTATTC AAACTCTTCA 
GCTTATCCTA AAGCTCAAGA TACTGCTGAT TACAGTACAC TGAGGATGCC ACGAAGCTCT 
TCCATAAGTA GAGGAGATAT ATGGGAAACT TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC 
CCGAAGAGAG TAGACATGCC ACCTGAAGAA GACTGGAGAC ATAATACTTT TACCACTCAA 
TTTGGTGGTA GGAAAACACA GCCTGTATTT TCACCCCACA GGACCTACTC ATCTGCTGCT 
GACCTATATG GGGCAAATAG CTGGAGAGCT GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915929.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915986.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915986.1
CDS	2319..7493
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915929.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915929.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915932.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915989.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915989.1
CDS	1412..6586
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915932.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915932.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915930.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915987.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915987.1
CDS	2223..7397
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915930.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915930.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915933.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915990.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915990.1
CDS	1316..6490
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915933.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915933.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915934.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915991.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915991.1
CDS	1232..6406
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915934.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915934.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_018095874.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951363.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_018095874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951363.1
CDS	2439..7613
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_018095874.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095874.1

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_012966848.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822302.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_012966848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822302.1
CDS	2259..7433
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_012966848.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966848.2

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_004915931.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915988.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.

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CloneID	OXa12828
Clone ID Related Accession (Same CDS sequence)	XM_004915929.3 , XM_004915932.3 , XM_004915930.3 , XM_004915933.3 , XM_004915934.3 , XM_018095874.1 , XM_012966848.2 , XM_004915931.3
Accession Version	XM_004915931.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915988.1
CDS	2139..7313
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKVTHGGDQSRESEDDELYSAENFRRLARSLSGTVISGKEEAVVSSQSFEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_004915931.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915931.3

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGTCACTCA TGGCGGGGAC CAAAGCAGGG AGTCTGAGGA CGATGAGCTG 
TACAGTGCAG AGAATTTCCG GCGCCTGGCT CGCAGCCTCA GTGGGACAGT CATCAGTGGC 
AAAGAAGAGG CAGTTGTCTC TTCTCAGAGC TTTGAGGCAC CCAATGAGAG GAAACCGCTT 
ATGGACAGCA GCCACTGCTC TCACAGCTCC TCCTTCCACC CTTATTCCCA AAATCCTTCT 
GTCTTATCCT TTGAACCCCA GCACTACCCA ACCCAGCTGC AGCACCCATC CTTTGAGGGA 
CTGGCGCCCA GCATGGTGGG TGCAGCACAC AGGCCATCAG GAGGTCCCAA CTTCCAAAAA 
GTTTTGGCTA TGTCTGACAG GGGTGAAAAT GTCCAAGGTA ACGAACAGTC GAGGATGGCA 
CTGAGCTATG CAACTTTTCC AAGGGCACCT AGGCTCAGTG CTACACCAAC TGCAACAGCA 
TCACAAAGAT CTTATGCTGT TCTCGGGCAG CAGACAAACA ATCGAGATCA AAATGTTCCA 
AGTCATTATC GGTGGTCAGG TCATACATCT CAAGGGTCTG CACCTGACAA ACACATGGTT 
AATTCCAGTT TCATGACTTC ACCTGGTTGG AGGTCACAAA GTCCTCAAAC CTCAGGTTAT 
GGAGGCTATT CAAACTCTTC AGCTTATCCT AAAGCTCAAG ATACTGCTGA TTACAGTACA 
CTGAGGATGC CACGAAGCTC TTCCATAAGT AGAGGAGATA TATGGGAAAC TTCTCAGGCT 
AATCAGTCAA GAGTTCAAGG CCCGAAGAGA GTAGACATGC CACCTGAAGA AGACTGGAGA 
CATAATACTT TTACCACTCA ATTTGGTGGT AGGAAAACAC AGCCTGTATT TTCACCCCAC 
AGGACCTACT CATCTGCTGC TGACCTATAT GGGGCAAATA GCTGGAGAGC TGCCTTGAAC 
AGAAATGGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_012966849.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822303.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.

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CloneID	OXa12830
Clone ID Related Accession (Same CDS sequence)	XM_012966849.2
Accession Version	XM_012966849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGAGGCACC CAATGAGAGG AAACCGCTTA TGGACAGCAG CCACTGCTCT 
CACAGCTCCT CCTTCCACCC TTATTCCCAA AATCCTTCTG TCTTATCCTT TGAACCCCAG 
CACTACCCAA CCCAGCTGCA GCACCCATCC TTTGAGGGAC TGGCGCCCAG CATGGTGGGT 
GCAGCACACA GGCCATCAGG AGGTCCCAAC TTCCAAAAAG TTTTGGCTAT GTCTGACAGG 
GGTGAAAATG TCCAAGGTAA CGAACAGTCG AGGATGGCAC TGAGCTATGC AACTTTTCCA 
AGGGCACCTA GGCTCAGTGC TACACCAACT GCAACAGCAT CACAAAGATC TTATGCTGTT 
CTCGGGCAGC AGACAAACAA TCGAGATCAA AATGTTCCAA GTCATTATCG GTGGTCAGGT 
CATACATCTC AAGGGTCTGC ACCTGACAAA CACATGGTTA ATTCCAGTTT CATGACTTCA 
CCTGGTTGGA GGTCACAAAG TCCTCAAACC TCAGGTTATG GAGGCTATTC AAACTCTTCA 
GCTTATCCTA AAGCTCAAGA TACTGCTGAT TACAGTACAC TGAGGATGCC ACGAAGCTCT 
TCCATAAGTA GAGGAGATAT ATGGGAAACT TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC 
CCGAAGAGAG TAGACATGCC ACCTGAAGAA GACTGGAGAC ATAATACTTT TACCACTCAA 
TTTGGTGGTA GGAAAACACA GCCTGTATTT TCACCCCACA GGACCTACTC ATCTGCTGCT 
GACCTATATG GGGCAAATAG CTGGAGAGCT GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822303.1
CDS	2446..7479
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKEAPNERKPLMDSSHCSHSSSFHPYSQNPSVLSFEPQHYPTQLQHPSFEGLAPSMVGAAHRPSGGPNFQKVLAMSDRGENVQGNEQSRMALSYATFPRAPRLSATPTATASQRSYAVLGQQTNNRDQNVPSHYRWSGHTSQGSAPDKHMVNSSFMTSPGWRSQSPQTSGYGGYSNSSAYPKAQDTADYSTLRMPRSSSISRGDIWETSQANQSRVQGPKRVDMPPEEDWRHNTFTTQFGGRKTQPVFSPHRTYSSAADLYGANSWRAALNRNGR

Target ORF information:

RefSeq Version	XM_012966849.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966849.2

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGGAGGCACC CAATGAGAGG AAACCGCTTA TGGACAGCAG CCACTGCTCT 
CACAGCTCCT CCTTCCACCC TTATTCCCAA AATCCTTCTG TCTTATCCTT TGAACCCCAG 
CACTACCCAA CCCAGCTGCA GCACCCATCC TTTGAGGGAC TGGCGCCCAG CATGGTGGGT 
GCAGCACACA GGCCATCAGG AGGTCCCAAC TTCCAAAAAG TTTTGGCTAT GTCTGACAGG 
GGTGAAAATG TCCAAGGTAA CGAACAGTCG AGGATGGCAC TGAGCTATGC AACTTTTCCA 
AGGGCACCTA GGCTCAGTGC TACACCAACT GCAACAGCAT CACAAAGATC TTATGCTGTT 
CTCGGGCAGC AGACAAACAA TCGAGATCAA AATGTTCCAA GTCATTATCG GTGGTCAGGT 
CATACATCTC AAGGGTCTGC ACCTGACAAA CACATGGTTA ATTCCAGTTT CATGACTTCA 
CCTGGTTGGA GGTCACAAAG TCCTCAAACC TCAGGTTATG GAGGCTATTC AAACTCTTCA 
GCTTATCCTA AAGCTCAAGA TACTGCTGAT TACAGTACAC TGAGGATGCC ACGAAGCTCT 
TCCATAAGTA GAGGAGATAT ATGGGAAACT TCTCAGGCTA ATCAGTCAAG AGTTCAAGGC 
CCGAAGAGAG TAGACATGCC ACCTGAAGAA GACTGGAGAC ATAATACTTT TACCACTCAA 
TTTGGTGGTA GGAAAACACA GCCTGTATTT TCACCCCACA GGACCTACTC ATCTGCTGCT 
GACCTATATG GGGCAAATAG CTGGAGAGCT GCCTTGAACA GAAATGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp54 ( XM_012966850.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822304.1 Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.

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CloneID	OXa36887
Clone ID Related Accession (Same CDS sequence)	XM_012966850.2
Accession Version	XM_012966850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGCTCTGGAC TAAACATGAA CTGATTTGGT TCAGAAACAG AGTTTTCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822304.1
CDS	2628..6890
Translation	MMSWKRNYFATGRGGVQEMFTPRNSISIAPSKGLINEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRCALAKTFQDEQRFQLGIMDDAAECFENLLIRLHIHIADESKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTALCNQAIFMLERKEKPTPDMFGELLQKASTMGDLRNCPSNCGEKIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFYRVTDDRGKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVTVPDVLSQMDLRQYTRTCYDSEDSGREPSISSDTRTDSSTDSYHYKHSHHESVVSYFSSESQGTVIYNLENDAASQSSRDTGHLTDSECSQRHFSRKGPFTDRKRSSSRPRRKGDESQSSGYHSEGETLKEKQAPRTGLKLSSSSSRLRDFKATVSNIIHNRPSQASQTSSQGFHHVGSNLDQSEIKPPTNLSMRLRDWDIDNSNNETKSGFSNRYRPTWRPTREPLNVDSVFRSEKRKNAAYSPLSPFSEDSGKEMATNEFREIANSESKDKHQFKYRSWGIGRGAPHFLDHQQPRLIQRMESGYESSERNSNSPISMDLPISENCSAFREYHSRKSPGTAPTWRNIPKSQSSSALDMADSASSVWVKVHPTHMAVESNVPMKSELDELQEEVARRAREQELRWKREKEIEAAMGFNPRPKRFLDLDELQNQGRSESFERSMQEADSVYEQSVRLEQKGDYTSALALCNEAISKIRFAMHDARSSTHCRAIADKKLQMCIRKARSLQDRMQLPTIPQPPSPDYPLPQGEAINQSTSDQAISEQVPVNRPVMLETNTKEDYPSNPLFHPLACSHELLPLSSESSNLSASEQSPHSKAFSNFLAPYNSLDSEGLVSSPKEAVFENKVKSEAYSQGLVNEQTKTERVKNVSPDKCKVGEENEGKEGLLTLHKVKLSGIKSGSLPSLLFPWARRTQTQSPDRSNPPSHCNKSVSTDKCVSTETGESNKQMGSCNDITPPGNTSLSLQQANLCSSANSHEALLDKQGELCGPEPCKSEAPKNKGLVRSLAEQFQRLQGGSPKLNNFGVNCTGVDIGEKKPLPEQQSFSPLDNKSSLQPLVIHENKASCTEQIDLSPDCLGRSFDIKGSSIHLVDNQNNGSEKKETPGWVVNAPLNQGKLFYKGGKKVKFASTVHLPGSVPMEQWAEDVTRYYNDKLESIGDKPKVGHTVSLKPKSIRYGLSKSSIDLRQEWNRDEEISDLQTLYNASLQACPNRNMQWRSENARQGFGKPLLENTAGRRVQTTAGKGLSRTPTKEMEHNLSGTTFSPVPKLWTKHELIWFRNRVFR

Target ORF information:

RefSeq Version	XM_012966850.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 54 (usp54), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966850.2

ORF Insert Sequence:

ATGATGTCCT GGAAGAGAAA TTATTTTGCT ACAGGTCGAG GAGGAGTTCA GGAGATGTTT 
ACACCACGCA ATTCAATATC CATTGCTCCT AGCAAAGGCC TGATTAATGA GCCAGGACAA 
AATAGCTGCT TCCTAAACAG TGCACTACAG GTGTTATGGC ATCTAGACAT ATTCCGCCGG 
AGCTTCCGAC AACTTACAAC ACACAAGTGT ATGGGAGACT CCTGTATATT CTGTGCACTG 
AAGGGAATCT TCAACCAGTT TCAGTGTAGC AGTGAGAAGG TGTTACCATC AGATACTCTT 
CGTTGTGCTC TTGCTAAAAC ATTTCAGGAT GAGCAGCGGT TCCAGTTGGG CATCATGGAT 
GATGCAGCAG AATGCTTTGA AAACTTATTA ATTCGCCTTC ACATCCACAT CGCAGATGAG 
TCCAAAGAAG ATATCTGTAC TGCCCAGCAT TGCATCTCCC ACCAGAAGTT TGCCATGACC 
TTGTTTGAGC AGTGTGTTTG TACAAGCTGT GGTGCCACTT CTGACCCCCT GCCTTTTATA 
CAGATGGTGC ATTATATATC TACTACTGCA CTCTGCAATC AGGCAATCTT TATGCTGGAG 
AGAAAAGAAA AACCCACCCC TGATATGTTT GGGGAACTGC TGCAAAAAGC CAGTACAATG 
GGAGACTTGA GAAACTGCCC GAGTAATTGT GGTGAAAAAA TCCGAATTCG GCGAGTGCTA 
ATGAATGCCC CTCAGATTAT CACCATTGGG CTGGTGTGGG ACTCAGATCA CTCAGATCTA 
GCCGAAGATG TTATTCACAG TCTTGGAACC TGCCTGAAGC TTGGTGATTT ATTTTACAGG 
GTAACCGATG ACCGAGGAAA ACATTCTGAG CTCTATTTAG TAGGGATGAT TTGTTACTAT 
GGCAAACATT ACTCTACTTT CTTTTTTCAA ACCAAAATAC GCAAGTGGAT GTATTTTGAT 
GATGCTCATG TTAAAGAGAT TGGTCCAAAA TGGAAAGATG TTGTTACAAA GTGTATAAAG 
GGTCATTATC AACCATTGCT GCTGCTGTAT GCTGATCCCA GGGGTACACC AGTAACAGTG 
CCTGATGTCC TGTCTCAGAT GGATCTACGT CAGTACACTA GAACATGCTA TGACAGTGAA 
GATTCTGGCA GGGAACCATC CATCTCCAGT GACACCAGAA CAGATTCTTC TACTGACAGT 
TACCATTATA AGCATTCTCA CCATGAGTCA GTGGTGAGCT ACTTCTCCTC TGAATCACAA 
GGCACAGTCA TCTACAATCT GGAAAATGAT GCAGCATCTC AAAGCAGCAG AGACACAGGA 
CACTTGACAG ATAGCGAGTG CAGCCAACGC CACTTTTCAA GAAAAGGACC CTTTACAGAC 
CGAAAACGAA GTTCCAGCCG CCCTCGGAGA AAAGGAGATG AGTCACAGTC TTCTGGCTAT 
CACAGCGAGG GTGAGACACT AAAGGAGAAG CAAGCACCCA GAACAGGCTT GAAACTGTCC 
AGCAGTTCTA GCAGGCTGAG GGATTTTAAA GCTACTGTTA GCAATATTAT ACATAACAGA 
CCATCCCAAG CATCACAGAC TTCTTCTCAA GGCTTTCACC ATGTGGGGAG TAACCTCGAT 
CAGTCAGAAA TCAAGCCACC TACAAACTTG TCAATGAGAC TGCGGGACTG GGATATAGAC 
AACAGCAACA ATGAAACAAA GTCTGGCTTC TCAAACAGGT ATCGCCCAAC CTGGCGGCCT 
ACTCGTGAGC CATTAAATGT AGACAGTGTC TTCAGGTCAG AAAAGAGGAA AAATGCTGCA 
TACAGTCCAC TTAGTCCTTT TTCAGAAGAT TCAGGAAAAG AAATGGCCAC TAATGAATTC 
CGAGAAATAG CCAACTCTGA ATCAAAAGAC AAGCATCAGT TTAAATACAG AAGTTGGGGC 
ATTGGGAGGG GTGCCCCACA CTTTCTTGAC CACCAGCAGC CTCGTCTTAT CCAGAGAATG 
GAGTCTGGGT ATGAAAGCAG TGAGCGGAAC AGCAATAGCC CAATCAGCAT GGACTTGCCA 
ATATCAGAGA ACTGTAGTGC ATTTAGGGAA TATCACTCAA GGAAAAGTCC AGGTACAGCT 
CCTACATGGC GAAACATTCC GAAATCTCAG AGCAGCAGTG CATTAGACAT GGCAGATTCT 
GCTTCCAGTG TCTGGGTGAA GGTCCATCCA ACACACATGG CAGTTGAAAG CAATGTACCT 
ATGAAGAGTG AACTTGATGA ACTACAGGAG GAGGTGGCAA GGAGGGCACG AGAACAGGAA 
CTGCGATGGA AGCGGGAGAA GGAAATTGAG GCTGCAATGG GATTCAATCC ACGACCAAAA 
CGGTTTTTGG ATTTAGATGA ATTACAGAAT CAGGGTAGAA GTGAAAGCTT TGAGCGATCT 
ATGCAAGAAG CAGATTCTGT GTATGAGCAG TCAGTGAGAC TGGAACAGAA GGGAGATTAC 
ACTTCAGCGC TGGCTTTGTG TAATGAAGCT ATTTCTAAAA TCAGATTTGC CATGCATGAT 
GCCAGATCTA GCACTCACTG CAGAGCCATA GCAGATAAGA AGTTGCAAAT GTGCATACGA 
AAAGCACGGA GTCTTCAGGA CCGCATGCAG CTGCCGACAA TTCCTCAGCC GCCATCACCA 
GATTACCCCT TGCCACAGGG GGAAGCTATA AACCAATCTA CAAGTGACCA GGCTATCTCG 
GAACAAGTTC CAGTGAACAG ACCGGTAATG CTGGAGACCA ACACAAAGGA GGACTACCCA 
TCCAATCCAT TATTCCACCC GTTAGCTTGT AGCCATGAGT TGCTTCCATT GTCCTCTGAG 
TCTTCCAACT TGTCAGCTTC TGAGCAAAGC CCACATAGCA AAGCGTTTTC TAACTTTCTG 
GCTCCATATA ACAGTCTCGA TTCTGAAGGT CTTGTCTCAT CTCCAAAGGA AGCAGTGTTT 
GAGAATAAGG TGAAATCAGA AGCTTATAGC CAAGGCCTAG TAAATGAACA AACAAAAACA 
GAAAGGGTAA AGAATGTCAG CCCTGATAAA TGTAAGGTGG GAGAGGAAAA TGAAGGAAAA 
GAAGGCCTTC TAACATTGCA TAAGGTGAAA CTAAGTGGGA TTAAATCTGG TTCTTTACCA 
TCATTGCTAT TTCCGTGGGC ACGCCGAACA CAGACTCAGT CTCCTGATAG AAGCAACCCA 
CCCAGTCATT GTAATAAATC TGTTAGCACA GACAAATGTG TTAGTACTGA AACTGGTGAA 
AGTAATAAAC AGATGGGTTC CTGTAATGAC ATCACTCCAC CTGGTAACAC CAGCCTCAGC 
CTTCAACAAG CCAACTTGTG CTCAAGTGCA AATAGTCATG AGGCATTATT AGACAAGCAA 
GGTGAACTTT GCGGTCCAGA ACCTTGCAAA TCTGAGGCAC CAAAGAATAA GGGGTTAGTA 
CGCTCTCTAG CAGAACAGTT TCAAAGGCTC CAGGGGGGCT CACCAAAACT AAACAATTTT 
GGTGTAAACT GTACTGGTGT TGATATTGGT GAGAAAAAGC CACTGCCAGA ACAGCAATCT 
TTCAGTCCTT TGGATAATAA ATCCAGTTTG CAGCCCCTTG TAATTCATGA AAACAAAGCT 
AGCTGCACAG AACAGATAGA CCTCTCACCA GACTGCCTGG GCAGGTCTTT TGACATAAAG 
GGTTCCAGTA TACATCTTGT TGATAACCAA AATAATGGCT CTGAAAAGAA AGAAACGCCT 
GGATGGGTTG TTAATGCTCC ACTTAACCAA GGTAAACTTT TCTATAAAGG AGGGAAAAAA 
GTTAAATTTG CAAGCACTGT CCATTTACCT GGCTCTGTGC CCATGGAGCA GTGGGCTGAA 
GATGTCACCA GATATTATAA TGATAAACTG GAGAGTATAG GTGATAAACC TAAAGTTGGA 
CACACTGTAT CTCTTAAACC AAAGTCAATA AGGTATGGCT TATCAAAAAG TTCAATTGAT 
CTGCGCCAGG AGTGGAATCG GGATGAAGAG ATCTCAGACC TGCAAACTTT ATATAATGCT 
AGCCTACAGG CTTGTCCAAA CCGGAATATG CAGTGGAGGT CAGAAAATGC AAGACAAGGA 
TTTGGAAAAC CACTTCTGGA AAACACAGCT GGAAGGAGGG TGCAGACAAC TGCTGGTAAA 
GGTCTTTCAA GGACGCCCAC AAAAGAAATG GAGCACAATC TTAGTGGAAC CACATTTTCA 
CCTGTGCCAA AGCTCTGGAC TAAACATGAA CTGATTTGGT TCAGAAACAG AGTTTTCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	usp54
Entrez Gene ID	100497431
Full Name	ubiquitin specific peptidase 54
General protein information	Preferred Names ubiquitin specific peptidase 54 Names inactive ubiquitin carboxyl-terminal hydrolase 54
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	USP54	NP_689799.3
Mus musculus (house mouse)	Usp54	NP_084456.2
Canis lupus familiaris (dog)	USP54	XP_005619078.1
Bos taurus (cattle)	USP54	XP_005226561.1
Gallus gallus (chicken)	USP54	XP_004942143.1
Danio rerio (zebrafish)	usp54a	NP_001188495.1
	LOC706859	XP_002808545.1
Rattus norvegicus (Norway rat)	Usp54	NP_001008863.2
Pan troglodytes (chimpanzee)	USP54	XP_003312678.1
Danio rerio (zebrafish)	LOC100535699	XP_003199659.2
Xenopus tropicalis (tropical clawed frog)	usp54	XP_002935811.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

usp54 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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