usp54b cDNA ORF clone, Danio rerio(zebrafish)

The following usp54b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp54b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa50221 XM_009306748.2
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa148356 XM_021480333.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa54513 XM_017358531.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa148352 XM_021480329.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148353 XM_021480330.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148354 XM_021480331.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148355 XM_021480332.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54511 XM_017358529.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54512 XM_017358530.1
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa50216 XM_009306743.2
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50217 XM_009306744.3
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50218 XM_009306745.3
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50219 XM_009306746.2
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50220 XM_009306747.2
Latest version!
Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa50217 XM_009306744.2 Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50218 XM_009306745.2 Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa50221
    Clone ID Related Accession (Same CDS sequence) XM_009306748.2
    Accession Version XM_009306748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGTGAA CAGCCTGGCC CGGTCCAAGT ATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305023.1
    CDS617..2884
    Translation

    Target ORF information:

    RefSeq Version XM_009306748.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGTGAA CAGCCTGGCC CGGTCCAAGT ATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148356
    Clone ID Related Accession (Same CDS sequence) XM_021480333.1
    Accession Version XM_021480333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGTGAACAG CCTGGCCCGG TCCAAGTATT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336008.1
    CDS624..2888
    Translation

    Target ORF information:

    RefSeq Version XM_021480333.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGTGAACAG CCTGGCCCGG TCCAAGTATT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54513
    Clone ID Related Accession (Same CDS sequence) XM_017358531.1
    Accession Version XM_017358531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTTGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214020.1
    CDS617..2980
    Translation

    Target ORF information:

    RefSeq Version XM_017358531.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTTGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148352
    Clone ID Related Accession (Same CDS sequence) XM_021480329.1
    Accession Version XM_021480329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336004.1
    CDS623..4471
    Translation

    Target ORF information:

    RefSeq Version XM_021480329.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148353
    Clone ID Related Accession (Same CDS sequence) XM_021480330.1
    Accession Version XM_021480330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTA TCTCCATCAT CATGCGTCAC CTTCACCCCT
    GTCTCCACAG CCTGGGCCTT GCAGAGAGGT TCCAGGATGT CTCCAGAGGT TCTCCGAGTT
    TCTGGCTGTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336005.1
    CDS623..4417
    Translation

    Target ORF information:

    RefSeq Version XM_021480330.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTA TCTCCATCAT CATGCGTCAC CTTCACCCCT
    GTCTCCACAG CCTGGGCCTT GCAGAGAGGT TCCAGGATGT CTCCAGAGGT TCTCCGAGTT
    TCTGGCTGTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148354
    Clone ID Related Accession (Same CDS sequence) XM_021480331.1
    Accession Version XM_021480331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTGAT GTCTCCAGAG GTTCTCCGAG TTTCTGGCTG
    TGTCTGACTG GTGTCAAACT CTCTCCAGTG GGGAGCACTA CCGTCCATCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336006.1
    CDS623..4396
    Translation

    Target ORF information:

    RefSeq Version XM_021480331.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTGAT GTCTCCAGAG GTTCTCCGAG TTTCTGGCTG
    TGTCTGACTG GTGTCAAACT CTCTCCAGTG GGGAGCACTA CCGTCCATCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148355
    Clone ID Related Accession (Same CDS sequence) XM_021480332.1
    Accession Version XM_021480332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGATT CGTGTATCTT CTGTGCTCTG AAGAGCATCT TCGCTCAGTT CCAGTTCAGC 
    AGTGAGAAGG TGCTCCCATC GGATGCTCTG CGCAGCGCTC TGGCCAAAAC CTTCCAAGAC
    GAGCAGCGCT TTCAGCTGGG CATCATGGAT GACGCGGCCG AGTGTTTCGA GAACCTCCTG
    ATGCGGATCC ACTTCCACAT CTCAGACGAG AGCAAGGAGG ACATCTGTAC GGCCAAACAC
    TGCATCCCAC ATCAGAAGTT TGCTATGACA CTTTTCGAAC AGTGTGTTTG TAATAATTGT
    GGAGCCACAT CAGATCCTCT CCCCTTCATT CAGATGGTGC ACTATATCTC CACCACCTCT
    CTCTGCAGTA ACCAAGCGGT GCGAATGCTG GAGTGTAAAG AGAAGCCCAC CCCTGACATG
    TTTGGAGAGC TTCTCCGCAA CGCCAGCACC ATGGGTGACC TGCGCAACTG CCCGAGTAAC
    TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT CTAATGAACT GCCCGGAGAT CATCAGCATC
    GGTCTTGTTT GGGATTCAGA TCACTCTGAT CTGGCTGAAG ATGTCATTCA CAGCCTGGGC
    ACATGCCTAC GCTTGGGTGA TCTCTTCTAC CGTGTGACGG ATGAACGAGC TAAGCAGTCT
    GAGCTCTATC TTGTGGGCAT GGTTTGCTAC TACGGCAAGC ATTACTCCAC CTTCTTCTTC
    CAGACCAAGA TACGCAAATG GATGTACTTT GATGACGCAC ATGTGAAGGA GATTGGTCCC
    AAGTGGAAGG ACGTGGTGTC GAGATGTATA AAAGGCCACT ACCAACCTCT ATTGCTGCTG
    TATGCTGACC CGCGTGGCAC ACCCGTGTCT TCGCAAGATA TGCTGGCAGC ACAGCTGGAC
    CTGCACCACA GCAGCAAGGC CGGCTACGAC AGCGAAGACT CCGGACGGGA GCCCTCAATT
    TCAAGTGACA CTCGCACAGA CTCTTCCACA GACAGCTACA GCTACAAGCA CTCTCGATCA
    CACCATGAGT CCATGGCCTC CCATTTCTCT TCTGACTCTC AGGGAACTGT GGTGTGTAAT
    CAGGACAGCA GCGGTTCCCA GAACAGCATG GACACTGCAG GGGAGGTTCT AGACCAGGCC
    ACTCCTATTT GCACGCCAAG ACCCTCTTCT ACTGGCCATC TGCAGGACTA TAAAGAGACT
    GTGAACATGA TCCACAGCAG ACCTCTCTCA TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG
    GAAGCTGGAG CGCGAGCGAG AGACTGGGAG GACGAGAGCA CCAGCAGCGA GTCCAAATCC
    AGCTCCAGCG GAGGCCGCTA TCGCCCCACT TGGAGACCCA GAAGAGAGGC CCTGAACATC
    GACAGCATCT TCAGCCGGGA AAGACATCGG CCTGTGGGAT ACACCACGCT TGGACCTTCC
    CCTCTGCCGG AAGACCCCAT GTCACCAGTG CACGGTGAGC TGGTCTCAGT GGCTGTGGCA
    GAGGGATCAA ACGGAGCAGC AGGAGAGACG GGGGATATGA CCGATGGCTC TGGGGGTGCG
    GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC CCACCACCTT TACGAGGTGT AGAACAGCAG
    CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC TACGAAAGTA GTGAGAGGAA CAGCAACAGC
    CCGATCAGCA TGGACATGCC CCTCAGCGAC AACCCAAACT CGAGTGCTCA CAGGGACTTT
    GGAAATAAGA GGCCTGTAAC GTCTGGACCA TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT
    AGCAGTGCCA TCCTGCAGGA CCTCAAATCA CCCACCTGGA GCAGCGCTGC GCTAAGTTTG
    GGGGAAGAGC GTAGTGAGCT GGATGAATTG CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA
    CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG AGAGAAGCGG CGCAGGGCTT TAATCCCAGA
    CCCAGCAAAT TCATGGACCT GGATGAACTT CAGCACCAGG GGAAGGGGGG CAGTTTTGAG
    CACTGCCTGG CGGAGGCTGA CTCTCTCATA GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG
    GACACAGCTA CAGCCCTGGC GCTCTCCCAC CACGCCGTGT CTAAACTAAG ATTTTCCATG
    CATGAGGGCA GTGCTAACAC TCATAGCAGG ACAATGGCTG CTGACGCCAA GCTGCAAAAA
    TGCATGAGGA GAGCGCGGGG CCTTCAGCAG CGCATGCAGC AACAGCAAGA ACAGCCGCAG
    GAGCCCAGCC GCCCACAGGG GCCCATCAGT GAACAGCCTG GCCCGGTCCA AGTATTGTTG
    ACAGACAATC AGGAAGAGGT CTGGGAGACC AATCCGGATT CAGTGCTTGG CCCACCCTCT
    CCTCAACACA TGAATTCACC CTCCTCTTGT ACAAACTCCC CATCTCAGCT CTCTAACCGC
    CCATCGCTCA ATCCAGGATC TTCCCAGCCA CATCCACAAG AGTCTGAGGT ATGCAGCAAT
    TGGTCAAAAC AGAAGGAGGA GGAATGGGGT GGCATCAGAT TATCAGACGT TCTCCAAGCA
    TCAGGCATTC GGGTCATGCC CATACGGAAA CACTTGGCCA TATCTCTACC CTCTTTGCCC
    CTCGACTTCT GGGGTGTATT GGGAGAGGAC GAAGAGCACC CTCAAGCATC GATCCCTCAA
    AATCCAAATC CTCCTTTGTG GCAGGACATT GCAGATAGTC CAGAGAAGGT ACGCCAATCC
    AACACGTCTA CCAGTCCAAC ACCTGCTACA AATGAGGAAA AGGGGTCAAT GCCCCGACGA
    GTCCCTATAT GGCAGCCTCT ATGTACATCT TCCCATCCGT GCAACAAACT GAGCCCGCCT
    GTTGCTGCCC GTCATTGGTC CTCTTGGAGT CTTGACCGTC CTGATGCTGT CATCACTCCC
    TATGACACAC CTCCTGAACA GAACCTACGG GCAGGAAGTG TCCTCTCCCA ACACACATCT
    GGAAGACACC TTCCTCCTCG CAGTCCCATG AGCACCAGGA AGAAGAATAT CTCTAAGCAC
    TCGAGCTTCC AAAGCATTGA TCTGGAGCCC ACTGAGGCTG AGGACCAGGA GCTCTCTGAA
    CTGGACTCAC TCTACCAGGC CAGTCTCCAG GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT
    GGTACCAGCT CACCTGCTGT TGGGAGATCA GTACCTCCTG CTGCTCGAAA ACTGCTCACT
    GGAGTCGGAC GCTCTCGAAC CCCTACGGCT GAACTCGAGA GAACCGCTTA TGGACCCCCA
    GGTTGTTACA GCCCTACTGG TTACATGAAC ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA
    CAGTCAGAAG AGGACGACCA GTATGATGCG GATCACCTGC GCCGTATTGC TCGCAGCCTG
    AGTGGAACCG TCATTGGGCG ACGCCAGAAC CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG
    GACTCTGGGG TTTCGTCATG TCAAAGCTTC ATGTACTCAC CTAGTTGCCT TCAGTTTCCA
    TTGTCTCATT CAGACCTCCA TCTGCTTCAA CCCAGCTATC TCCATCATCA TGCGTCACCT
    TCACCCCTGT CTCCACAGCC TGGGCCTTGC AGAGAGGTTC CAGGATGTCT CCAGAGGTTC
    TCCGAGTTTC TGGCTGTGTC TGACTGGTGT CAAACTCTCT CCAGTGGGGA GCACTACCGT
    CCATCACTGA GGGTTCCTGG AGGACGTCCT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336007.1
    CDS413..4168
    Translation

    Target ORF information:

    RefSeq Version XM_021480332.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGATT CGTGTATCTT CTGTGCTCTG AAGAGCATCT TCGCTCAGTT CCAGTTCAGC 
    AGTGAGAAGG TGCTCCCATC GGATGCTCTG CGCAGCGCTC TGGCCAAAAC CTTCCAAGAC
    GAGCAGCGCT TTCAGCTGGG CATCATGGAT GACGCGGCCG AGTGTTTCGA GAACCTCCTG
    ATGCGGATCC ACTTCCACAT CTCAGACGAG AGCAAGGAGG ACATCTGTAC GGCCAAACAC
    TGCATCCCAC ATCAGAAGTT TGCTATGACA CTTTTCGAAC AGTGTGTTTG TAATAATTGT
    GGAGCCACAT CAGATCCTCT CCCCTTCATT CAGATGGTGC ACTATATCTC CACCACCTCT
    CTCTGCAGTA ACCAAGCGGT GCGAATGCTG GAGTGTAAAG AGAAGCCCAC CCCTGACATG
    TTTGGAGAGC TTCTCCGCAA CGCCAGCACC ATGGGTGACC TGCGCAACTG CCCGAGTAAC
    TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT CTAATGAACT GCCCGGAGAT CATCAGCATC
    GGTCTTGTTT GGGATTCAGA TCACTCTGAT CTGGCTGAAG ATGTCATTCA CAGCCTGGGC
    ACATGCCTAC GCTTGGGTGA TCTCTTCTAC CGTGTGACGG ATGAACGAGC TAAGCAGTCT
    GAGCTCTATC TTGTGGGCAT GGTTTGCTAC TACGGCAAGC ATTACTCCAC CTTCTTCTTC
    CAGACCAAGA TACGCAAATG GATGTACTTT GATGACGCAC ATGTGAAGGA GATTGGTCCC
    AAGTGGAAGG ACGTGGTGTC GAGATGTATA AAAGGCCACT ACCAACCTCT ATTGCTGCTG
    TATGCTGACC CGCGTGGCAC ACCCGTGTCT TCGCAAGATA TGCTGGCAGC ACAGCTGGAC
    CTGCACCACA GCAGCAAGGC CGGCTACGAC AGCGAAGACT CCGGACGGGA GCCCTCAATT
    TCAAGTGACA CTCGCACAGA CTCTTCCACA GACAGCTACA GCTACAAGCA CTCTCGATCA
    CACCATGAGT CCATGGCCTC CCATTTCTCT TCTGACTCTC AGGGAACTGT GGTGTGTAAT
    CAGGACAGCA GCGGTTCCCA GAACAGCATG GACACTGCAG GGGAGGTTCT AGACCAGGCC
    ACTCCTATTT GCACGCCAAG ACCCTCTTCT ACTGGCCATC TGCAGGACTA TAAAGAGACT
    GTGAACATGA TCCACAGCAG ACCTCTCTCA TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG
    GAAGCTGGAG CGCGAGCGAG AGACTGGGAG GACGAGAGCA CCAGCAGCGA GTCCAAATCC
    AGCTCCAGCG GAGGCCGCTA TCGCCCCACT TGGAGACCCA GAAGAGAGGC CCTGAACATC
    GACAGCATCT TCAGCCGGGA AAGACATCGG CCTGTGGGAT ACACCACGCT TGGACCTTCC
    CCTCTGCCGG AAGACCCCAT GTCACCAGTG CACGGTGAGC TGGTCTCAGT GGCTGTGGCA
    GAGGGATCAA ACGGAGCAGC AGGAGAGACG GGGGATATGA CCGATGGCTC TGGGGGTGCG
    GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC CCACCACCTT TACGAGGTGT AGAACAGCAG
    CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC TACGAAAGTA GTGAGAGGAA CAGCAACAGC
    CCGATCAGCA TGGACATGCC CCTCAGCGAC AACCCAAACT CGAGTGCTCA CAGGGACTTT
    GGAAATAAGA GGCCTGTAAC GTCTGGACCA TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT
    AGCAGTGCCA TCCTGCAGGA CCTCAAATCA CCCACCTGGA GCAGCGCTGC GCTAAGTTTG
    GGGGAAGAGC GTAGTGAGCT GGATGAATTG CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA
    CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG AGAGAAGCGG CGCAGGGCTT TAATCCCAGA
    CCCAGCAAAT TCATGGACCT GGATGAACTT CAGCACCAGG GGAAGGGGGG CAGTTTTGAG
    CACTGCCTGG CGGAGGCTGA CTCTCTCATA GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG
    GACACAGCTA CAGCCCTGGC GCTCTCCCAC CACGCCGTGT CTAAACTAAG ATTTTCCATG
    CATGAGGGCA GTGCTAACAC TCATAGCAGG ACAATGGCTG CTGACGCCAA GCTGCAAAAA
    TGCATGAGGA GAGCGCGGGG CCTTCAGCAG CGCATGCAGC AACAGCAAGA ACAGCCGCAG
    GAGCCCAGCC GCCCACAGGG GCCCATCAGT GAACAGCCTG GCCCGGTCCA AGTATTGTTG
    ACAGACAATC AGGAAGAGGT CTGGGAGACC AATCCGGATT CAGTGCTTGG CCCACCCTCT
    CCTCAACACA TGAATTCACC CTCCTCTTGT ACAAACTCCC CATCTCAGCT CTCTAACCGC
    CCATCGCTCA ATCCAGGATC TTCCCAGCCA CATCCACAAG AGTCTGAGGT ATGCAGCAAT
    TGGTCAAAAC AGAAGGAGGA GGAATGGGGT GGCATCAGAT TATCAGACGT TCTCCAAGCA
    TCAGGCATTC GGGTCATGCC CATACGGAAA CACTTGGCCA TATCTCTACC CTCTTTGCCC
    CTCGACTTCT GGGGTGTATT GGGAGAGGAC GAAGAGCACC CTCAAGCATC GATCCCTCAA
    AATCCAAATC CTCCTTTGTG GCAGGACATT GCAGATAGTC CAGAGAAGGT ACGCCAATCC
    AACACGTCTA CCAGTCCAAC ACCTGCTACA AATGAGGAAA AGGGGTCAAT GCCCCGACGA
    GTCCCTATAT GGCAGCCTCT ATGTACATCT TCCCATCCGT GCAACAAACT GAGCCCGCCT
    GTTGCTGCCC GTCATTGGTC CTCTTGGAGT CTTGACCGTC CTGATGCTGT CATCACTCCC
    TATGACACAC CTCCTGAACA GAACCTACGG GCAGGAAGTG TCCTCTCCCA ACACACATCT
    GGAAGACACC TTCCTCCTCG CAGTCCCATG AGCACCAGGA AGAAGAATAT CTCTAAGCAC
    TCGAGCTTCC AAAGCATTGA TCTGGAGCCC ACTGAGGCTG AGGACCAGGA GCTCTCTGAA
    CTGGACTCAC TCTACCAGGC CAGTCTCCAG GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT
    GGTACCAGCT CACCTGCTGT TGGGAGATCA GTACCTCCTG CTGCTCGAAA ACTGCTCACT
    GGAGTCGGAC GCTCTCGAAC CCCTACGGCT GAACTCGAGA GAACCGCTTA TGGACCCCCA
    GGTTGTTACA GCCCTACTGG TTACATGAAC ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA
    CAGTCAGAAG AGGACGACCA GTATGATGCG GATCACCTGC GCCGTATTGC TCGCAGCCTG
    AGTGGAACCG TCATTGGGCG ACGCCAGAAC CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG
    GACTCTGGGG TTTCGTCATG TCAAAGCTTC ATGTACTCAC CTAGTTGCCT TCAGTTTCCA
    TTGTCTCATT CAGACCTCCA TCTGCTTCAA CCCAGCTATC TCCATCATCA TGCGTCACCT
    TCACCCCTGT CTCCACAGCC TGGGCCTTGC AGAGAGGTTC CAGGATGTCT CCAGAGGTTC
    TCCGAGTTTC TGGCTGTGTC TGACTGGTGT CAAACTCTCT CCAGTGGGGA GCACTACCGT
    CCATCACTGA GGGTTCCTGG AGGACGTCCT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54511
    Clone ID Related Accession (Same CDS sequence) XM_017358529.1
    Accession Version XM_017358529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTATCTCCAT CATCATGCGT CACCTTCACC
    CCTGTCTCCA CAGCCTGGGC CTTGCAGAGA GGTTCCAGGA TGTCTCCAGA GGTTCTCCGA
    GTTTCTGGCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214018.1
    CDS617..4414
    Translation

    Target ORF information:

    RefSeq Version XM_017358529.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTATCTCCAT CATCATGCGT CACCTTCACC
    CCTGTCTCCA CAGCCTGGGC CTTGCAGAGA GGTTCCAGGA TGTCTCCAGA GGTTCTCCGA
    GTTTCTGGCT GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54512
    Clone ID Related Accession (Same CDS sequence) XM_017358530.1
    Accession Version XM_017358530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT GATGTCTCCA GAGGTTCTCC GAGTTTCTGG
    CTGTGTCTGA CTGGTGTCAA ACTCTCTCCA GTGGGGAGCA CTACCGTCCA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214019.1
    CDS617..4393
    Translation

    Target ORF information:

    RefSeq Version XM_017358530.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT GATGTCTCCA GAGGTTCTCC GAGTTTCTGG
    CTGTGTCTGA CTGGTGTCAA ACTCTCTCCA GTGGGGAGCA CTACCGTCCA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50216
    Clone ID Related Accession (Same CDS sequence) XM_009306743.2
    Accession Version XM_009306743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTCGGGGACT CTGGGGTTTC GTCATGTCAA
    AGCTTCATGT ACTCACCTAG TTGCCTTCAG TTTCCATTGT CTCATTCAGA CCTCCATCTG
    CTTCAACCCA GCTATCTCCA TCATCATGCG TCACCTTCAC CCCTGTCTCC ACAGCCTGGG
    CCTTGCAGAG AGGTTCCAGG ATGTCTCCAG AGGTTCTCCG AGTTTCTGGC TGTGTCTGAC
    TGGTGTCAAA CTCTCTCCAG TGGGGAGCAC TACCGTCCAT CACTGAGGGT TCCTGGAGGA
    CGTCCTGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305018.1
    CDS617..4588
    Translation

    Target ORF information:

    RefSeq Version XM_009306743.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGGGAA GGGGGGCAGT TTTGAGCACT GCCTGGCGGA GGCTGACTCT
    CTCATAGAGC AGTCGATGCG CCTGGAGCAG GCTGGGGACA CAGCTACAGC CCTGGCGCTC
    TCCCACCACG CCGTGTCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTCGGGGACT CTGGGGTTTC GTCATGTCAA
    AGCTTCATGT ACTCACCTAG TTGCCTTCAG TTTCCATTGT CTCATTCAGA CCTCCATCTG
    CTTCAACCCA GCTATCTCCA TCATCATGCG TCACCTTCAC CCCTGTCTCC ACAGCCTGGG
    CCTTGCAGAG AGGTTCCAGG ATGTCTCCAG AGGTTCTCCG AGTTTCTGGC TGTGTCTGAC
    TGGTGTCAAA CTCTCTCCAG TGGGGAGCAC TACCGTCCAT CACTGAGGGT TCCTGGAGGA
    CGTCCTGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50217
    Clone ID Related Accession (Same CDS sequence) XM_009306744.2 , XM_009306744.3
    Accession Version XM_009306744.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305019.1
    CDS617..4585
    Translation

    Target ORF information:

    RefSeq Version XM_009306744.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306744.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50217
    Clone ID Related Accession (Same CDS sequence) XM_009306744.2 , XM_009306744.3
    Accession Version XM_009306744.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305019.1
    CDS624..4592
    Translation

    Target ORF information:

    RefSeq Version XM_009306744.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306744.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCA GTAACCAAGC GGTGCGAATG
    CTGGAGTGTA AAGAGAAGCC CACCCCTGAC ATGTTTGGAG AGCTTCTCCG CAACGCCAGC
    ACCATGGGTG ACCTGCGCAA CTGCCCGAGT AACTGTGGTG AGATGCTGCG GATTCGGCGA
    GTTCTAATGA ACTGCCCGGA GATCATCAGC ATCGGTCTTG TTTGGGATTC AGATCACTCT
    GATCTGGCTG AAGATGTCAT TCACAGCCTG GGCACATGCC TACGCTTGGG TGATCTCTTC
    TACCGTGTGA CGGATGAACG AGCTAAGCAG TCTGAGCTCT ATCTTGTGGG CATGGTTTGC
    TACTACGGCA AGCATTACTC CACCTTCTTC TTCCAGACCA AGATACGCAA ATGGATGTAC
    TTTGATGACG CACATGTGAA GGAGATTGGT CCCAAGTGGA AGGACGTGGT GTCGAGATGT
    ATAAAAGGCC ACTACCAACC TCTATTGCTG CTGTATGCTG ACCCGCGTGG CACACCCGTG
    TCTTCGCAAG ATATGCTGGC AGCACAGCTG GACCTGCACC ACAGCAGCAA GGCCGGCTAC
    GACAGCGAAG ACTCCGGACG GGAGCCCTCA ATTTCAAGTG ACACTCGCAC AGACTCTTCC
    ACAGACAGCT ACAGCTACAA GCACTCTCGA TCACACCATG AGTCCATGGC CTCCCATTTC
    TCTTCTGACT CTCAGGGAAC TGTGGTGTGT AATCAGGACA GCAGCGGTTC CCAGAACAGC
    ATGGACACTG CAGGGGAGGT TCTAGACCAG GCCACTCCTA TTTGCACGCC AAGACCCTCT
    TCTACTGGCC ATCTGCAGGA CTATAAAGAG ACTGTGAACA TGATCCACAG CAGACCTCTC
    TCATCGTCCT CTAGCTCAGG CGTGGGGGGT TCGGAAGCTG GAGCGCGAGC GAGAGACTGG
    GAGGACGAGA GCACCAGCAG CGAGTCCAAA TCCAGCTCCA GCGGAGGCCG CTATCGCCCC
    ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC ATCGACAGCA TCTTCAGCCG GGAAAGACAT
    CGGCCTGTGG GATACACCAC GCTTGGACCT TCCCCTCTGC CGGAAGACCC CATGTCACCA
    GTGCACGGTG AGCTGGTCTC AGTGGCTGTG GCAGAGGGAT CAAACGGAGC AGCAGGAGAG
    ACGGGGGATA TGACCGATGG CTCTGGGGGT GCGGTGGAGC CGGGAGTTGA GCATGTGCCA
    AACCCACCAC CTTTACGAGG TGTAGAACAG CAGCCTCGGC TGATCCAGAG GATGGAGAGT
    GGCTACGAAA GTAGTGAGAG GAACAGCAAC AGCCCGATCA GCATGGACAT GCCCCTCAGC
    GACAACCCAA ACTCGAGTGC TCACAGGGAC TTTGGAAATA AGAGGCCTGT AACGTCTGGA
    CCATCTTGGC GCACTGTGCC CAAGTCCAAA AGTAGCAGTG CCATCCTGCA GGACCTCAAA
    TCACCCACCT GGAGCAGCGC TGCGCTAAGT TTGGGGGAAG AGCGTAGTGA GCTGGATGAA
    TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG CAACAGGAGC TGCAGAGGAA GAAGGAGAAG
    GAGAGAGAAG CGGCGCAGGG CTTTAATCCC AGACCCAGCA AATTCATGGA CCTGGATGAA
    CTTCAGCACC AGGGGAAGGG GGGCAGTTTT GAGCACTGCC TGGCGGAGGC TGACTCTCTC
    ATAGAGCAGT CGATGCGCCT GGAGCAGGCT GGGGACACAG CTACAGCCCT GGCGCTCTCC
    CACCACGCCG TGTCTAAACT AAGATTTTCC ATGCATGAGG GCAGTGCTAA CACTCATAGC
    AGGACAATGG CTGCTGACGC CAAGCTGCAA AAATGCATGA GGAGAGCGCG GGGCCTTCAG
    CAGCGCATGC AGCAACAGCA AGAACAGCCG CAGGAGCCCA GCCGCCCACA GGGGCCCATC
    AGTGAACAGC CTGGCCCGGT CCAAGTATTG TTGACAGACA ATCAGGAAGA GGTCTGGGAG
    ACCAATCCGG ATTCAGTGCT TGGCCCACCC TCTCCTCAAC ACATGAATTC ACCCTCCTCT
    TGTACAAACT CCCCATCTCA GCTCTCTAAC CGCCCATCGC TCAATCCAGG ATCTTCCCAG
    CCACATCCAC AAGAGTCTGA GGTATGCAGC AATTGGTCAA AACAGAAGGA GGAGGAATGG
    GGTGGCATCA GATTATCAGA CGTTCTCCAA GCATCAGGCA TTCGGGTCAT GCCCATACGG
    AAACACTTGG CCATATCTCT ACCCTCTTTG CCCCTCGACT TCTGGGGTGT ATTGGGAGAG
    GACGAAGAGC ACCCTCAAGC ATCGATCCCT CAAAATCCAA ATCCTCCTTT GTGGCAGGAC
    ATTGCAGATA GTCCAGAGAA GGTACGCCAA TCCAACACGT CTACCAGTCC AACACCTGCT
    ACAAATGAGG AAAAGGGGTC AATGCCCCGA CGAGTCCCTA TATGGCAGCC TCTATGTACA
    TCTTCCCATC CGTGCAACAA ACTGAGCCCG CCTGTTGCTG CCCGTCATTG GTCCTCTTGG
    AGTCTTGACC GTCCTGATGC TGTCATCACT CCCTATGACA CACCTCCTGA ACAGAACCTA
    CGGGCAGGAA GTGTCCTCTC CCAACACACA TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC
    ATGAGCACCA GGAAGAAGAA TATCTCTAAG CACTCGAGCT TCCAAAGCAT TGATCTGGAG
    CCCACTGAGG CTGAGGACCA GGAGCTCTCT GAACTGGACT CACTCTACCA GGCCAGTCTC
    CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG CGTGGTACCA GCTCACCTGC TGTTGGGAGA
    TCAGTACCTC CTGCTGCTCG AAAACTGCTC ACTGGAGTCG GACGCTCTCG AACCCCTACG
    GCTGAACTCG AGAGAACCGC TTATGGACCC CCAGGTTGTT ACAGCCCTAC TGGTTACATG
    AACATGCCTC TGTCGGTGGC CCATCAAGGG CTACAGTCAG AAGAGGACGA CCAGTATGAT
    GCGGATCACC TGCGCCGTAT TGCTCGCAGC CTGAGTGGAA CCGTCATTGG GCGACGCCAG
    AACCGTCTTG CTGTCTCCCG AAGCTTTCTC GGGGACTCTG GGGTTTCGTC ATGTCAAAGC
    TTCATGTACT CACCTAGTTG CCTTCAGTTT CCATTGTCTC ATTCAGACCT CCATCTGCTT
    CAACCCAGCT ATCTCCATCA TCATGCGTCA CCTTCACCCC TGTCTCCACA GCCTGGGCCT
    TGCAGAGAGG TTCCAGGATG TCTCCAGAGG TTCTCCGAGT TTCTGGCTGT GTCTGACTGG
    TGTCAAACTC TCTCCAGTGG GGAGCACTAC CGTCCATCAC TGAGGGTTCC TGGAGGACGT
    CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50218
    Clone ID Related Accession (Same CDS sequence) XM_009306745.3 , XM_009306745.2
    Accession Version XM_009306745.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGTA ACCAAGCGGT GCGAATGCTG
    GAGTGTAAAG AGAAGCCCAC CCCTGACATG TTTGGAGAGC TTCTCCGCAA CGCCAGCACC
    ATGGGTGACC TGCGCAACTG CCCGAGTAAC TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT
    CTAATGAACT GCCCGGAGAT CATCAGCATC GGTCTTGTTT GGGATTCAGA TCACTCTGAT
    CTGGCTGAAG ATGTCATTCA CAGCCTGGGC ACATGCCTAC GCTTGGGTGA TCTCTTCTAC
    CGTGTGACGG ATGAACGAGC TAAGCAGTCT GAGCTCTATC TTGTGGGCAT GGTTTGCTAC
    TACGGCAAGC ATTACTCCAC CTTCTTCTTC CAGACCAAGA TACGCAAATG GATGTACTTT
    GATGACGCAC ATGTGAAGGA GATTGGTCCC AAGTGGAAGG ACGTGGTGTC GAGATGTATA
    AAAGGCCACT ACCAACCTCT ATTGCTGCTG TATGCTGACC CGCGTGGCAC ACCCGTGTCT
    TCGCAAGATA TGCTGGCAGC ACAGCTGGAC CTGCACCACA GCAGCAAGGC CGGCTACGAC
    AGCGAAGACT CCGGACGGGA GCCCTCAATT TCAAGTGACA CTCGCACAGA CTCTTCCACA
    GACAGCTACA GCTACAAGCA CTCTCGATCA CACCATGAGT CCATGGCCTC CCATTTCTCT
    TCTGACTCTC AGGGAACTGT GGTGTGTAAT CAGGACAGCA GCGGTTCCCA GAACAGCATG
    GACACTGCAG GGGAGGTTCT AGACCAGGCC ACTCCTATTT GCACGCCAAG ACCCTCTTCT
    ACTGGCCATC TGCAGGACTA TAAAGAGACT GTGAACATGA TCCACAGCAG ACCTCTCTCA
    TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG GAAGCTGGAG CGCGAGCGAG AGACTGGGAG
    GACGAGAGCA CCAGCAGCGA GTCCAAATCC AGCTCCAGCG GAGGCCGCTA TCGCCCCACT
    TGGAGACCCA GAAGAGAGGC CCTGAACATC GACAGCATCT TCAGCCGGGA AAGACATCGG
    CCTGTGGGAT ACACCACGCT TGGACCTTCC CCTCTGCCGG AAGACCCCAT GTCACCAGTG
    CACGGTGAGC TGGTCTCAGT GGCTGTGGCA GAGGGATCAA ACGGAGCAGC AGGAGAGACG
    GGGGATATGA CCGATGGCTC TGGGGGTGCG GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC
    CCACCACCTT TACGAGGTGT AGAACAGCAG CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC
    TACGAAAGTA GTGAGAGGAA CAGCAACAGC CCGATCAGCA TGGACATGCC CCTCAGCGAC
    AACCCAAACT CGAGTGCTCA CAGGGACTTT GGAAATAAGA GGCCTGTAAC GTCTGGACCA
    TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT AGCAGTGCCA TCCTGCAGGA CCTCAAATCA
    CCCACCTGGA GCAGCGCTGC GCTAAGTTTG GGGGAAGAGC GTAGTGAGCT GGATGAATTG
    CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG
    AGAGAAGCGG CGCAGGGCTT TAATCCCAGA CCCAGCAAAT TCATGGACCT GGATGAACTT
    CAGCACCAGG GGAAGGGGGG CAGTTTTGAG CACTGCCTGG CGGAGGCTGA CTCTCTCATA
    GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG GACACAGCTA CAGCCCTGGC GCTCTCCCAC
    CACGCCGTGT CTAAACTAAG ATTTTCCATG CATGAGGGCA GTGCTAACAC TCATAGCAGG
    ACAATGGCTG CTGACGCCAA GCTGCAAAAA TGCATGAGGA GAGCGCGGGG CCTTCAGCAG
    CGCATGCAGC AACAGCAAGA ACAGCCGCAG GAGCCCAGCC GCCCACAGGG GCCCATCAGT
    GAACAGCCTG GCCCGGTCCA AGTATTGTTG ACAGACAATC AGGAAGAGGT CTGGGAGACC
    AATCCGGATT CAGTGCTTGG CCCACCCTCT CCTCAACACA TGAATTCACC CTCCTCTTGT
    ACAAACTCCC CATCTCAGCT CTCTAACCGC CCATCGCTCA ATCCAGGATC TTCCCAGCCA
    CATCCACAAG AGTCTGAGGT ATGCAGCAAT TGGTCAAAAC AGAAGGAGGA GGAATGGGGT
    GGCATCAGAT TATCAGACGT TCTCCAAGCA TCAGGCATTC GGGTCATGCC CATACGGAAA
    CACTTGGCCA TATCTCTACC CTCTTTGCCC CTCGACTTCT GGGGTGTATT GGGAGAGGAC
    GAAGAGCACC CTCAAGCATC GATCCCTCAA AATCCAAATC CTCCTTTGTG GCAGGACATT
    GCAGATAGTC CAGAGAAGGT ACGCCAATCC AACACGTCTA CCAGTCCAAC ACCTGCTACA
    AATGAGGAAA AGGGGTCAAT GCCCCGACGA GTCCCTATAT GGCAGCCTCT ATGTACATCT
    TCCCATCCGT GCAACAAACT GAGCCCGCCT GTTGCTGCCC GTCATTGGTC CTCTTGGAGT
    CTTGACCGTC CTGATGCTGT CATCACTCCC TATGACACAC CTCCTGAACA GAACCTACGG
    GCAGGAAGTG TCCTCTCCCA ACACACATCT GGAAGACACC TTCCTCCTCG CAGTCCCATG
    AGCACCAGGA AGAAGAATAT CTCTAAGCAC TCGAGCTTCC AAAGCATTGA TCTGGAGCCC
    ACTGAGGCTG AGGACCAGGA GCTCTCTGAA CTGGACTCAC TCTACCAGGC CAGTCTCCAG
    GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT GGTACCAGCT CACCTGCTGT TGGGAGATCA
    GTACCTCCTG CTGCTCGAAA ACTGCTCACT GGAGTCGGAC GCTCTCGAAC CCCTACGGCT
    GAACTCGAGA GAACCGCTTA TGGACCCCCA GGTTGTTACA GCCCTACTGG TTACATGAAC
    ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA CAGTCAGAAG AGGACGACCA GTATGATGCG
    GATCACCTGC GCCGTATTGC TCGCAGCCTG AGTGGAACCG TCATTGGGCG ACGCCAGAAC
    CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG GACTCTGGGG TTTCGTCATG TCAAAGCTTC
    ATGTACTCAC CTAGTTGCCT TCAGTTTCCA TTGTCTCATT CAGACCTCCA TCTGCTTCAA
    CCCAGCTATC TCCATCATCA TGCGTCACCT TCACCCCTGT CTCCACAGCC TGGGCCTTGC
    AGAGAGGTTC CAGGATGTCT CCAGAGGTTC TCCGAGTTTC TGGCTGTGTC TGACTGGTGT
    CAAACTCTCT CCAGTGGGGA GCACTACCGT CCATCACTGA GGGTTCCTGG AGGACGTCCT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305020.1
    CDS623..4588
    Translation

    Target ORF information:

    RefSeq Version XM_009306745.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGTA ACCAAGCGGT GCGAATGCTG
    GAGTGTAAAG AGAAGCCCAC CCCTGACATG TTTGGAGAGC TTCTCCGCAA CGCCAGCACC
    ATGGGTGACC TGCGCAACTG CCCGAGTAAC TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT
    CTAATGAACT GCCCGGAGAT CATCAGCATC GGTCTTGTTT GGGATTCAGA TCACTCTGAT
    CTGGCTGAAG ATGTCATTCA CAGCCTGGGC ACATGCCTAC GCTTGGGTGA TCTCTTCTAC
    CGTGTGACGG ATGAACGAGC TAAGCAGTCT GAGCTCTATC TTGTGGGCAT GGTTTGCTAC
    TACGGCAAGC ATTACTCCAC CTTCTTCTTC CAGACCAAGA TACGCAAATG GATGTACTTT
    GATGACGCAC ATGTGAAGGA GATTGGTCCC AAGTGGAAGG ACGTGGTGTC GAGATGTATA
    AAAGGCCACT ACCAACCTCT ATTGCTGCTG TATGCTGACC CGCGTGGCAC ACCCGTGTCT
    TCGCAAGATA TGCTGGCAGC ACAGCTGGAC CTGCACCACA GCAGCAAGGC CGGCTACGAC
    AGCGAAGACT CCGGACGGGA GCCCTCAATT TCAAGTGACA CTCGCACAGA CTCTTCCACA
    GACAGCTACA GCTACAAGCA CTCTCGATCA CACCATGAGT CCATGGCCTC CCATTTCTCT
    TCTGACTCTC AGGGAACTGT GGTGTGTAAT CAGGACAGCA GCGGTTCCCA GAACAGCATG
    GACACTGCAG GGGAGGTTCT AGACCAGGCC ACTCCTATTT GCACGCCAAG ACCCTCTTCT
    ACTGGCCATC TGCAGGACTA TAAAGAGACT GTGAACATGA TCCACAGCAG ACCTCTCTCA
    TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG GAAGCTGGAG CGCGAGCGAG AGACTGGGAG
    GACGAGAGCA CCAGCAGCGA GTCCAAATCC AGCTCCAGCG GAGGCCGCTA TCGCCCCACT
    TGGAGACCCA GAAGAGAGGC CCTGAACATC GACAGCATCT TCAGCCGGGA AAGACATCGG
    CCTGTGGGAT ACACCACGCT TGGACCTTCC CCTCTGCCGG AAGACCCCAT GTCACCAGTG
    CACGGTGAGC TGGTCTCAGT GGCTGTGGCA GAGGGATCAA ACGGAGCAGC AGGAGAGACG
    GGGGATATGA CCGATGGCTC TGGGGGTGCG GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC
    CCACCACCTT TACGAGGTGT AGAACAGCAG CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC
    TACGAAAGTA GTGAGAGGAA CAGCAACAGC CCGATCAGCA TGGACATGCC CCTCAGCGAC
    AACCCAAACT CGAGTGCTCA CAGGGACTTT GGAAATAAGA GGCCTGTAAC GTCTGGACCA
    TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT AGCAGTGCCA TCCTGCAGGA CCTCAAATCA
    CCCACCTGGA GCAGCGCTGC GCTAAGTTTG GGGGAAGAGC GTAGTGAGCT GGATGAATTG
    CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG
    AGAGAAGCGG CGCAGGGCTT TAATCCCAGA CCCAGCAAAT TCATGGACCT GGATGAACTT
    CAGCACCAGG GGAAGGGGGG CAGTTTTGAG CACTGCCTGG CGGAGGCTGA CTCTCTCATA
    GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG GACACAGCTA CAGCCCTGGC GCTCTCCCAC
    CACGCCGTGT CTAAACTAAG ATTTTCCATG CATGAGGGCA GTGCTAACAC TCATAGCAGG
    ACAATGGCTG CTGACGCCAA GCTGCAAAAA TGCATGAGGA GAGCGCGGGG CCTTCAGCAG
    CGCATGCAGC AACAGCAAGA ACAGCCGCAG GAGCCCAGCC GCCCACAGGG GCCCATCAGT
    GAACAGCCTG GCCCGGTCCA AGTATTGTTG ACAGACAATC AGGAAGAGGT CTGGGAGACC
    AATCCGGATT CAGTGCTTGG CCCACCCTCT CCTCAACACA TGAATTCACC CTCCTCTTGT
    ACAAACTCCC CATCTCAGCT CTCTAACCGC CCATCGCTCA ATCCAGGATC TTCCCAGCCA
    CATCCACAAG AGTCTGAGGT ATGCAGCAAT TGGTCAAAAC AGAAGGAGGA GGAATGGGGT
    GGCATCAGAT TATCAGACGT TCTCCAAGCA TCAGGCATTC GGGTCATGCC CATACGGAAA
    CACTTGGCCA TATCTCTACC CTCTTTGCCC CTCGACTTCT GGGGTGTATT GGGAGAGGAC
    GAAGAGCACC CTCAAGCATC GATCCCTCAA AATCCAAATC CTCCTTTGTG GCAGGACATT
    GCAGATAGTC CAGAGAAGGT ACGCCAATCC AACACGTCTA CCAGTCCAAC ACCTGCTACA
    AATGAGGAAA AGGGGTCAAT GCCCCGACGA GTCCCTATAT GGCAGCCTCT ATGTACATCT
    TCCCATCCGT GCAACAAACT GAGCCCGCCT GTTGCTGCCC GTCATTGGTC CTCTTGGAGT
    CTTGACCGTC CTGATGCTGT CATCACTCCC TATGACACAC CTCCTGAACA GAACCTACGG
    GCAGGAAGTG TCCTCTCCCA ACACACATCT GGAAGACACC TTCCTCCTCG CAGTCCCATG
    AGCACCAGGA AGAAGAATAT CTCTAAGCAC TCGAGCTTCC AAAGCATTGA TCTGGAGCCC
    ACTGAGGCTG AGGACCAGGA GCTCTCTGAA CTGGACTCAC TCTACCAGGC CAGTCTCCAG
    GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT GGTACCAGCT CACCTGCTGT TGGGAGATCA
    GTACCTCCTG CTGCTCGAAA ACTGCTCACT GGAGTCGGAC GCTCTCGAAC CCCTACGGCT
    GAACTCGAGA GAACCGCTTA TGGACCCCCA GGTTGTTACA GCCCTACTGG TTACATGAAC
    ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA CAGTCAGAAG AGGACGACCA GTATGATGCG
    GATCACCTGC GCCGTATTGC TCGCAGCCTG AGTGGAACCG TCATTGGGCG ACGCCAGAAC
    CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG GACTCTGGGG TTTCGTCATG TCAAAGCTTC
    ATGTACTCAC CTAGTTGCCT TCAGTTTCCA TTGTCTCATT CAGACCTCCA TCTGCTTCAA
    CCCAGCTATC TCCATCATCA TGCGTCACCT TCACCCCTGT CTCCACAGCC TGGGCCTTGC
    AGAGAGGTTC CAGGATGTCT CCAGAGGTTC TCCGAGTTTC TGGCTGTGTC TGACTGGTGT
    CAAACTCTCT CCAGTGGGGA GCACTACCGT CCATCACTGA GGGTTCCTGG AGGACGTCCT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50218
    Clone ID Related Accession (Same CDS sequence) XM_009306745.3 , XM_009306745.2
    Accession Version XM_009306745.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGTA ACCAAGCGGT GCGAATGCTG
    GAGTGTAAAG AGAAGCCCAC CCCTGACATG TTTGGAGAGC TTCTCCGCAA CGCCAGCACC
    ATGGGTGACC TGCGCAACTG CCCGAGTAAC TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT
    CTAATGAACT GCCCGGAGAT CATCAGCATC GGTCTTGTTT GGGATTCAGA TCACTCTGAT
    CTGGCTGAAG ATGTCATTCA CAGCCTGGGC ACATGCCTAC GCTTGGGTGA TCTCTTCTAC
    CGTGTGACGG ATGAACGAGC TAAGCAGTCT GAGCTCTATC TTGTGGGCAT GGTTTGCTAC
    TACGGCAAGC ATTACTCCAC CTTCTTCTTC CAGACCAAGA TACGCAAATG GATGTACTTT
    GATGACGCAC ATGTGAAGGA GATTGGTCCC AAGTGGAAGG ACGTGGTGTC GAGATGTATA
    AAAGGCCACT ACCAACCTCT ATTGCTGCTG TATGCTGACC CGCGTGGCAC ACCCGTGTCT
    TCGCAAGATA TGCTGGCAGC ACAGCTGGAC CTGCACCACA GCAGCAAGGC CGGCTACGAC
    AGCGAAGACT CCGGACGGGA GCCCTCAATT TCAAGTGACA CTCGCACAGA CTCTTCCACA
    GACAGCTACA GCTACAAGCA CTCTCGATCA CACCATGAGT CCATGGCCTC CCATTTCTCT
    TCTGACTCTC AGGGAACTGT GGTGTGTAAT CAGGACAGCA GCGGTTCCCA GAACAGCATG
    GACACTGCAG GGGAGGTTCT AGACCAGGCC ACTCCTATTT GCACGCCAAG ACCCTCTTCT
    ACTGGCCATC TGCAGGACTA TAAAGAGACT GTGAACATGA TCCACAGCAG ACCTCTCTCA
    TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG GAAGCTGGAG CGCGAGCGAG AGACTGGGAG
    GACGAGAGCA CCAGCAGCGA GTCCAAATCC AGCTCCAGCG GAGGCCGCTA TCGCCCCACT
    TGGAGACCCA GAAGAGAGGC CCTGAACATC GACAGCATCT TCAGCCGGGA AAGACATCGG
    CCTGTGGGAT ACACCACGCT TGGACCTTCC CCTCTGCCGG AAGACCCCAT GTCACCAGTG
    CACGGTGAGC TGGTCTCAGT GGCTGTGGCA GAGGGATCAA ACGGAGCAGC AGGAGAGACG
    GGGGATATGA CCGATGGCTC TGGGGGTGCG GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC
    CCACCACCTT TACGAGGTGT AGAACAGCAG CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC
    TACGAAAGTA GTGAGAGGAA CAGCAACAGC CCGATCAGCA TGGACATGCC CCTCAGCGAC
    AACCCAAACT CGAGTGCTCA CAGGGACTTT GGAAATAAGA GGCCTGTAAC GTCTGGACCA
    TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT AGCAGTGCCA TCCTGCAGGA CCTCAAATCA
    CCCACCTGGA GCAGCGCTGC GCTAAGTTTG GGGGAAGAGC GTAGTGAGCT GGATGAATTG
    CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG
    AGAGAAGCGG CGCAGGGCTT TAATCCCAGA CCCAGCAAAT TCATGGACCT GGATGAACTT
    CAGCACCAGG GGAAGGGGGG CAGTTTTGAG CACTGCCTGG CGGAGGCTGA CTCTCTCATA
    GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG GACACAGCTA CAGCCCTGGC GCTCTCCCAC
    CACGCCGTGT CTAAACTAAG ATTTTCCATG CATGAGGGCA GTGCTAACAC TCATAGCAGG
    ACAATGGCTG CTGACGCCAA GCTGCAAAAA TGCATGAGGA GAGCGCGGGG CCTTCAGCAG
    CGCATGCAGC AACAGCAAGA ACAGCCGCAG GAGCCCAGCC GCCCACAGGG GCCCATCAGT
    GAACAGCCTG GCCCGGTCCA AGTATTGTTG ACAGACAATC AGGAAGAGGT CTGGGAGACC
    AATCCGGATT CAGTGCTTGG CCCACCCTCT CCTCAACACA TGAATTCACC CTCCTCTTGT
    ACAAACTCCC CATCTCAGCT CTCTAACCGC CCATCGCTCA ATCCAGGATC TTCCCAGCCA
    CATCCACAAG AGTCTGAGGT ATGCAGCAAT TGGTCAAAAC AGAAGGAGGA GGAATGGGGT
    GGCATCAGAT TATCAGACGT TCTCCAAGCA TCAGGCATTC GGGTCATGCC CATACGGAAA
    CACTTGGCCA TATCTCTACC CTCTTTGCCC CTCGACTTCT GGGGTGTATT GGGAGAGGAC
    GAAGAGCACC CTCAAGCATC GATCCCTCAA AATCCAAATC CTCCTTTGTG GCAGGACATT
    GCAGATAGTC CAGAGAAGGT ACGCCAATCC AACACGTCTA CCAGTCCAAC ACCTGCTACA
    AATGAGGAAA AGGGGTCAAT GCCCCGACGA GTCCCTATAT GGCAGCCTCT ATGTACATCT
    TCCCATCCGT GCAACAAACT GAGCCCGCCT GTTGCTGCCC GTCATTGGTC CTCTTGGAGT
    CTTGACCGTC CTGATGCTGT CATCACTCCC TATGACACAC CTCCTGAACA GAACCTACGG
    GCAGGAAGTG TCCTCTCCCA ACACACATCT GGAAGACACC TTCCTCCTCG CAGTCCCATG
    AGCACCAGGA AGAAGAATAT CTCTAAGCAC TCGAGCTTCC AAAGCATTGA TCTGGAGCCC
    ACTGAGGCTG AGGACCAGGA GCTCTCTGAA CTGGACTCAC TCTACCAGGC CAGTCTCCAG
    GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT GGTACCAGCT CACCTGCTGT TGGGAGATCA
    GTACCTCCTG CTGCTCGAAA ACTGCTCACT GGAGTCGGAC GCTCTCGAAC CCCTACGGCT
    GAACTCGAGA GAACCGCTTA TGGACCCCCA GGTTGTTACA GCCCTACTGG TTACATGAAC
    ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA CAGTCAGAAG AGGACGACCA GTATGATGCG
    GATCACCTGC GCCGTATTGC TCGCAGCCTG AGTGGAACCG TCATTGGGCG ACGCCAGAAC
    CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG GACTCTGGGG TTTCGTCATG TCAAAGCTTC
    ATGTACTCAC CTAGTTGCCT TCAGTTTCCA TTGTCTCATT CAGACCTCCA TCTGCTTCAA
    CCCAGCTATC TCCATCATCA TGCGTCACCT TCACCCCTGT CTCCACAGCC TGGGCCTTGC
    AGAGAGGTTC CAGGATGTCT CCAGAGGTTC TCCGAGTTTC TGGCTGTGTC TGACTGGTGT
    CAAACTCTCT CCAGTGGGGA GCACTACCGT CCATCACTGA GGGTTCCTGG AGGACGTCCT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305020.1
    CDS617..4582
    Translation

    Target ORF information:

    RefSeq Version XM_009306745.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGTA ACCAAGCGGT GCGAATGCTG
    GAGTGTAAAG AGAAGCCCAC CCCTGACATG TTTGGAGAGC TTCTCCGCAA CGCCAGCACC
    ATGGGTGACC TGCGCAACTG CCCGAGTAAC TGTGGTGAGA TGCTGCGGAT TCGGCGAGTT
    CTAATGAACT GCCCGGAGAT CATCAGCATC GGTCTTGTTT GGGATTCAGA TCACTCTGAT
    CTGGCTGAAG ATGTCATTCA CAGCCTGGGC ACATGCCTAC GCTTGGGTGA TCTCTTCTAC
    CGTGTGACGG ATGAACGAGC TAAGCAGTCT GAGCTCTATC TTGTGGGCAT GGTTTGCTAC
    TACGGCAAGC ATTACTCCAC CTTCTTCTTC CAGACCAAGA TACGCAAATG GATGTACTTT
    GATGACGCAC ATGTGAAGGA GATTGGTCCC AAGTGGAAGG ACGTGGTGTC GAGATGTATA
    AAAGGCCACT ACCAACCTCT ATTGCTGCTG TATGCTGACC CGCGTGGCAC ACCCGTGTCT
    TCGCAAGATA TGCTGGCAGC ACAGCTGGAC CTGCACCACA GCAGCAAGGC CGGCTACGAC
    AGCGAAGACT CCGGACGGGA GCCCTCAATT TCAAGTGACA CTCGCACAGA CTCTTCCACA
    GACAGCTACA GCTACAAGCA CTCTCGATCA CACCATGAGT CCATGGCCTC CCATTTCTCT
    TCTGACTCTC AGGGAACTGT GGTGTGTAAT CAGGACAGCA GCGGTTCCCA GAACAGCATG
    GACACTGCAG GGGAGGTTCT AGACCAGGCC ACTCCTATTT GCACGCCAAG ACCCTCTTCT
    ACTGGCCATC TGCAGGACTA TAAAGAGACT GTGAACATGA TCCACAGCAG ACCTCTCTCA
    TCGTCCTCTA GCTCAGGCGT GGGGGGTTCG GAAGCTGGAG CGCGAGCGAG AGACTGGGAG
    GACGAGAGCA CCAGCAGCGA GTCCAAATCC AGCTCCAGCG GAGGCCGCTA TCGCCCCACT
    TGGAGACCCA GAAGAGAGGC CCTGAACATC GACAGCATCT TCAGCCGGGA AAGACATCGG
    CCTGTGGGAT ACACCACGCT TGGACCTTCC CCTCTGCCGG AAGACCCCAT GTCACCAGTG
    CACGGTGAGC TGGTCTCAGT GGCTGTGGCA GAGGGATCAA ACGGAGCAGC AGGAGAGACG
    GGGGATATGA CCGATGGCTC TGGGGGTGCG GTGGAGCCGG GAGTTGAGCA TGTGCCAAAC
    CCACCACCTT TACGAGGTGT AGAACAGCAG CCTCGGCTGA TCCAGAGGAT GGAGAGTGGC
    TACGAAAGTA GTGAGAGGAA CAGCAACAGC CCGATCAGCA TGGACATGCC CCTCAGCGAC
    AACCCAAACT CGAGTGCTCA CAGGGACTTT GGAAATAAGA GGCCTGTAAC GTCTGGACCA
    TCTTGGCGCA CTGTGCCCAA GTCCAAAAGT AGCAGTGCCA TCCTGCAGGA CCTCAAATCA
    CCCACCTGGA GCAGCGCTGC GCTAAGTTTG GGGGAAGAGC GTAGTGAGCT GGATGAATTG
    CAGGAGGAAG TTGCTCGCAG GGCTAGGCAA CAGGAGCTGC AGAGGAAGAA GGAGAAGGAG
    AGAGAAGCGG CGCAGGGCTT TAATCCCAGA CCCAGCAAAT TCATGGACCT GGATGAACTT
    CAGCACCAGG GGAAGGGGGG CAGTTTTGAG CACTGCCTGG CGGAGGCTGA CTCTCTCATA
    GAGCAGTCGA TGCGCCTGGA GCAGGCTGGG GACACAGCTA CAGCCCTGGC GCTCTCCCAC
    CACGCCGTGT CTAAACTAAG ATTTTCCATG CATGAGGGCA GTGCTAACAC TCATAGCAGG
    ACAATGGCTG CTGACGCCAA GCTGCAAAAA TGCATGAGGA GAGCGCGGGG CCTTCAGCAG
    CGCATGCAGC AACAGCAAGA ACAGCCGCAG GAGCCCAGCC GCCCACAGGG GCCCATCAGT
    GAACAGCCTG GCCCGGTCCA AGTATTGTTG ACAGACAATC AGGAAGAGGT CTGGGAGACC
    AATCCGGATT CAGTGCTTGG CCCACCCTCT CCTCAACACA TGAATTCACC CTCCTCTTGT
    ACAAACTCCC CATCTCAGCT CTCTAACCGC CCATCGCTCA ATCCAGGATC TTCCCAGCCA
    CATCCACAAG AGTCTGAGGT ATGCAGCAAT TGGTCAAAAC AGAAGGAGGA GGAATGGGGT
    GGCATCAGAT TATCAGACGT TCTCCAAGCA TCAGGCATTC GGGTCATGCC CATACGGAAA
    CACTTGGCCA TATCTCTACC CTCTTTGCCC CTCGACTTCT GGGGTGTATT GGGAGAGGAC
    GAAGAGCACC CTCAAGCATC GATCCCTCAA AATCCAAATC CTCCTTTGTG GCAGGACATT
    GCAGATAGTC CAGAGAAGGT ACGCCAATCC AACACGTCTA CCAGTCCAAC ACCTGCTACA
    AATGAGGAAA AGGGGTCAAT GCCCCGACGA GTCCCTATAT GGCAGCCTCT ATGTACATCT
    TCCCATCCGT GCAACAAACT GAGCCCGCCT GTTGCTGCCC GTCATTGGTC CTCTTGGAGT
    CTTGACCGTC CTGATGCTGT CATCACTCCC TATGACACAC CTCCTGAACA GAACCTACGG
    GCAGGAAGTG TCCTCTCCCA ACACACATCT GGAAGACACC TTCCTCCTCG CAGTCCCATG
    AGCACCAGGA AGAAGAATAT CTCTAAGCAC TCGAGCTTCC AAAGCATTGA TCTGGAGCCC
    ACTGAGGCTG AGGACCAGGA GCTCTCTGAA CTGGACTCAC TCTACCAGGC CAGTCTCCAG
    GCTGGCCCGG CTCCTGGTCG AGCCTTGCGT GGTACCAGCT CACCTGCTGT TGGGAGATCA
    GTACCTCCTG CTGCTCGAAA ACTGCTCACT GGAGTCGGAC GCTCTCGAAC CCCTACGGCT
    GAACTCGAGA GAACCGCTTA TGGACCCCCA GGTTGTTACA GCCCTACTGG TTACATGAAC
    ATGCCTCTGT CGGTGGCCCA TCAAGGGCTA CAGTCAGAAG AGGACGACCA GTATGATGCG
    GATCACCTGC GCCGTATTGC TCGCAGCCTG AGTGGAACCG TCATTGGGCG ACGCCAGAAC
    CGTCTTGCTG TCTCCCGAAG CTTTCTCGGG GACTCTGGGG TTTCGTCATG TCAAAGCTTC
    ATGTACTCAC CTAGTTGCCT TCAGTTTCCA TTGTCTCATT CAGACCTCCA TCTGCTTCAA
    CCCAGCTATC TCCATCATCA TGCGTCACCT TCACCCCTGT CTCCACAGCC TGGGCCTTGC
    AGAGAGGTTC CAGGATGTCT CCAGAGGTTC TCCGAGTTTC TGGCTGTGTC TGACTGGTGT
    CAAACTCTCT CCAGTGGGGA GCACTACCGT CCATCACTGA GGGTTCCTGG AGGACGTCCT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50219
    Clone ID Related Accession (Same CDS sequence) XM_009306746.2
    Accession Version XM_009306746.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTCGGGGACT CTGGGGTTTC GTCATGTCAA
    AGCTTCATGT ACTCACCTAG TTGCCTTCAG TTTCCATTGT CTCATTCAGA CCTCCATCTG
    CTTCAACCCA GCTATCTCCA TCATCATGCG TCACCTTCAC CCCTGTCTCC ACAGCCTGGG
    CCTTGCAGAG AGGTTCCAGG ATGTCTCCAG AGGTTCTCCG AGTTTCTGGC TGTGTCTGAC
    TGGTGTCAAA CTCTCTCCAG TGGGGAGCAC TACCGTCCAT CACTGAGGGT TCCTGGAGGA
    CGTCCTGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305021.1
    CDS617..4468
    Translation

    Target ORF information:

    RefSeq Version XM_009306746.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306746.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGTGGA AGAGGAACTA CTTTGCGTCA GGCAGCGGCG GCGGCAGCCT GCAGGGGATG 
    TTCACCCCAC GCAACATGAC CTCCATCGCT CCCAGCAAAG GCCTGAGCAA TGAGCCGGGC
    CAGAACAGCT GCTTTCTGAA CAGCGCGCTA CAGGTCCTTT GGCATCTTGA CATCTTCCGC
    AGAAGCTTCC GGCAACTGAA CACTCACAAG TGCATGGAGG ATTCGTGTAT CTTCTGTGCT
    CTGAAGAGCA TCTTCGCTCA GTTCCAGTTC AGCAGTGAGA AGGTGCTCCC ATCGGATGCT
    CTGCGCAGCG CTCTGGCCAA AACCTTCCAA GACGAGCAGC GCTTTCAGCT GGGCATCATG
    GATGACGCGG CCGAGTGTTT CGAGAACCTC CTGATGCGGA TCCACTTCCA CATCTCAGAC
    GAGAGCAAGG AGGACATCTG TACGGCCAAA CACTGCATCC CACATCAGAA GTTTGCTATG
    ACACTTTTCG AACAGTGTGT TTGTAATAAT TGTGGAGCCA CATCAGATCC TCTCCCCTTC
    ATTCAGATGG TGCACTATAT CTCCACCACC TCTCTCTGCT GCAGTAACCA AGCGGTGCGA
    ATGCTGGAGT GTAAAGAGAA GCCCACCCCT GACATGTTTG GAGAGCTTCT CCGCAACGCC
    AGCACCATGG GTGACCTGCG CAACTGCCCG AGTAACTGTG GTGAGATGCT GCGGATTCGG
    CGAGTTCTAA TGAACTGCCC GGAGATCATC AGCATCGGTC TTGTTTGGGA TTCAGATCAC
    TCTGATCTGG CTGAAGATGT CATTCACAGC CTGGGCACAT GCCTACGCTT GGGTGATCTC
    TTCTACCGTG TGACGGATGA ACGAGCTAAG CAGTCTGAGC TCTATCTTGT GGGCATGGTT
    TGCTACTACG GCAAGCATTA CTCCACCTTC TTCTTCCAGA CCAAGATACG CAAATGGATG
    TACTTTGATG ACGCACATGT GAAGGAGATT GGTCCCAAGT GGAAGGACGT GGTGTCGAGA
    TGTATAAAAG GCCACTACCA ACCTCTATTG CTGCTGTATG CTGACCCGCG TGGCACACCC
    GTGTCTTCGC AAGATATGCT GGCAGCACAG CTGGACCTGC ACCACAGCAG CAAGGCCGGC
    TACGACAGCG AAGACTCCGG ACGGGAGCCC TCAATTTCAA GTGACACTCG CACAGACTCT
    TCCACAGACA GCTACAGCTA CAAGCACTCT CGATCACACC ATGAGTCCAT GGCCTCCCAT
    TTCTCTTCTG ACTCTCAGGG AACTGTGGTG TGTAATCAGG ACAGCAGCGG TTCCCAGAAC
    AGCATGGACA CTGCAGGGGA GGTTCTAGAC CAGGCCACTC CTATTTGCAC GCCAAGACCC
    TCTTCTACTG GCCATCTGCA GGACTATAAA GAGACTGTGA ACATGATCCA CAGCAGACCT
    CTCTCATCGT CCTCTAGCTC AGGCGTGGGG GGTTCGGAAG CTGGAGCGCG AGCGAGAGAC
    TGGGAGGACG AGAGCACCAG CAGCGAGTCC AAATCCAGCT CCAGCGGAGG CCGCTATCGC
    CCCACTTGGA GACCCAGAAG AGAGGCCCTG AACATCGACA GCATCTTCAG CCGGGAAAGA
    CATCGGCCTG TGGGATACAC CACGCTTGGA CCTTCCCCTC TGCCGGAAGA CCCCATGTCA
    CCAGTGCACG GTGAGCTGGT CTCAGTGGCT GTGGCAGAGG GATCAAACGG AGCAGCAGGA
    GAGACGGGGG ATATGACCGA TGGCTCTGGG GGTGCGGTGG AGCCGGGAGT TGAGCATGTG
    CCAAACCCAC CACCTTTACG AGGTGTAGAA CAGCAGCCTC GGCTGATCCA GAGGATGGAG
    AGTGGCTACG AAAGTAGTGA GAGGAACAGC AACAGCCCGA TCAGCATGGA CATGCCCCTC
    AGCGACAACC CAAACTCGAG TGCTCACAGG GACTTTGGAA ATAAGAGGCC TGTAACGTCT
    GGACCATCTT GGCGCACTGT GCCCAAGTCC AAAAGTAGCA GTGCCATCCT GCAGGACCTC
    AAATCACCCA CCTGGAGCAG CGCTGCGCTA AGTTTGGGGG AAGAGCGTAG TGAGCTGGAT
    GAATTGCAGG AGGAAGTTGC TCGCAGGGCT AGGCAACAGG AGCTGCAGAG GAAGAAGGAG
    AAGGAGAGAG AAGCGGCGCA GGGCTTTAAT CCCAGACCCA GCAAATTCAT GGACCTGGAT
    GAACTTCAGC ACCAGGCTAA ACTAAGATTT TCCATGCATG AGGGCAGTGC TAACACTCAT
    AGCAGGACAA TGGCTGCTGA CGCCAAGCTG CAAAAATGCA TGAGGAGAGC GCGGGGCCTT
    CAGCAGCGCA TGCAGCAACA GCAAGAACAG CCGCAGGAGC CCAGCCGCCC ACAGGGGCCC
    ATCAGTGAAC AGCCTGGCCC GGTCCAAGTA TTGTTGACAG ACAATCAGGA AGAGGTCTGG
    GAGACCAATC CGGATTCAGT GCTTGGCCCA CCCTCTCCTC AACACATGAA TTCACCCTCC
    TCTTGTACAA ACTCCCCATC TCAGCTCTCT AACCGCCCAT CGCTCAATCC AGGATCTTCC
    CAGCCACATC CACAAGAGTC TGAGGTATGC AGCAATTGGT CAAAACAGAA GGAGGAGGAA
    TGGGGTGGCA TCAGATTATC AGACGTTCTC CAAGCATCAG GCATTCGGGT CATGCCCATA
    CGGAAACACT TGGCCATATC TCTACCCTCT TTGCCCCTCG ACTTCTGGGG TGTATTGGGA
    GAGGACGAAG AGCACCCTCA AGCATCGATC CCTCAAAATC CAAATCCTCC TTTGTGGCAG
    GACATTGCAG ATAGTCCAGA GAAGGTACGC CAATCCAACA CGTCTACCAG TCCAACACCT
    GCTACAAATG AGGAAAAGGG GTCAATGCCC CGACGAGTCC CTATATGGCA GCCTCTATGT
    ACATCTTCCC ATCCGTGCAA CAAACTGAGC CCGCCTGTTG CTGCCCGTCA TTGGTCCTCT
    TGGAGTCTTG ACCGTCCTGA TGCTGTCATC ACTCCCTATG ACACACCTCC TGAACAGAAC
    CTACGGGCAG GAAGTGTCCT CTCCCAACAC ACATCTGGAA GACACCTTCC TCCTCGCAGT
    CCCATGAGCA CCAGGAAGAA GAATATCTCT AAGCACTCGA GCTTCCAAAG CATTGATCTG
    GAGCCCACTG AGGCTGAGGA CCAGGAGCTC TCTGAACTGG ACTCACTCTA CCAGGCCAGT
    CTCCAGGCTG GCCCGGCTCC TGGTCGAGCC TTGCGTGGTA CCAGCTCACC TGCTGTTGGG
    AGATCAGTAC CTCCTGCTGC TCGAAAACTG CTCACTGGAG TCGGACGCTC TCGAACCCCT
    ACGGCTGAAC TCGAGAGAAC CGCTTATGGA CCCCCAGGTT GTTACAGCCC TACTGGTTAC
    ATGAACATGC CTCTGTCGGT GGCCCATCAA GGGCTACAGT CAGAAGAGGA CGACCAGTAT
    GATGCGGATC ACCTGCGCCG TATTGCTCGC AGCCTGAGTG GAACCGTCAT TGGGCGACGC
    CAGAACCGTC TTGCTGTCTC CCGAAGCTTT CTCGGGGACT CTGGGGTTTC GTCATGTCAA
    AGCTTCATGT ACTCACCTAG TTGCCTTCAG TTTCCATTGT CTCATTCAGA CCTCCATCTG
    CTTCAACCCA GCTATCTCCA TCATCATGCG TCACCTTCAC CCCTGTCTCC ACAGCCTGGG
    CCTTGCAGAG AGGTTCCAGG ATGTCTCCAG AGGTTCTCCG AGTTTCTGGC TGTGTCTGAC
    TGGTGTCAAA CTCTCTCCAG TGGGGAGCAC TACCGTCCAT CACTGAGGGT TCCTGGAGGA
    CGTCCTGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50220
    Clone ID Related Accession (Same CDS sequence) XM_009306747.2
    Accession Version XM_009306747.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGATT CGTGTATCTT CTGTGCTCTG AAGAGCATCT TCGCTCAGTT CCAGTTCAGC 
    AGTGAGAAGG TGCTCCCATC GGATGCTCTG CGCAGCGCTC TGGCCAAAAC CTTCCAAGAC
    GAGCAGCGCT TTCAGCTGGG CATCATGGAT GACGCGGCCG AGTGTTTCGA GAACCTCCTG
    ATGCGGATCC ACTTCCACAT CTCAGACGAG AGCAAGGAGG ACATCTGTAC GGCCAAACAC
    TGCATCCCAC ATCAGAAGTT TGCTATGACA CTTTTCGAAC AGTGTGTTTG TAATAATTGT
    GGAGCCACAT CAGATCCTCT CCCCTTCATT CAGATGGTGC ACTATATCTC CACCACCTCT
    CTCTGCTGCA GTAACCAAGC GGTGCGAATG CTGGAGTGTA AAGAGAAGCC CACCCCTGAC
    ATGTTTGGAG AGCTTCTCCG CAACGCCAGC ACCATGGGTG ACCTGCGCAA CTGCCCGAGT
    AACTGTGGTG AGATGCTGCG GATTCGGCGA GTTCTAATGA ACTGCCCGGA GATCATCAGC
    ATCGGTCTTG TTTGGGATTC AGATCACTCT GATCTGGCTG AAGATGTCAT TCACAGCCTG
    GGCACATGCC TACGCTTGGG TGATCTCTTC TACCGTGTGA CGGATGAACG AGCTAAGCAG
    TCTGAGCTCT ATCTTGTGGG CATGGTTTGC TACTACGGCA AGCATTACTC CACCTTCTTC
    TTCCAGACCA AGATACGCAA ATGGATGTAC TTTGATGACG CACATGTGAA GGAGATTGGT
    CCCAAGTGGA AGGACGTGGT GTCGAGATGT ATAAAAGGCC ACTACCAACC TCTATTGCTG
    CTGTATGCTG ACCCGCGTGG CACACCCGTG TCTTCGCAAG ATATGCTGGC AGCACAGCTG
    GACCTGCACC ACAGCAGCAA GGCCGGCTAC GACAGCGAAG ACTCCGGACG GGAGCCCTCA
    ATTTCAAGTG ACACTCGCAC AGACTCTTCC ACAGACAGCT ACAGCTACAA GCACTCTCGA
    TCACACCATG AGTCCATGGC CTCCCATTTC TCTTCTGACT CTCAGGGAAC TGTGGTGTGT
    AATCAGGACA GCAGCGGTTC CCAGAACAGC ATGGACACTG CAGGGGAGGT TCTAGACCAG
    GCCACTCCTA TTTGCACGCC AAGACCCTCT TCTACTGGCC ATCTGCAGGA CTATAAAGAG
    ACTGTGAACA TGATCCACAG CAGACCTCTC TCATCGTCCT CTAGCTCAGG CGTGGGGGGT
    TCGGAAGCTG GAGCGCGAGC GAGAGACTGG GAGGACGAGA GCACCAGCAG CGAGTCCAAA
    TCCAGCTCCA GCGGAGGCCG CTATCGCCCC ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC
    ATCGACAGCA TCTTCAGCCG GGAAAGACAT CGGCCTGTGG GATACACCAC GCTTGGACCT
    TCCCCTCTGC CGGAAGACCC CATGTCACCA GTGCACGGTG AGCTGGTCTC AGTGGCTGTG
    GCAGAGGGAT CAAACGGAGC AGCAGGAGAG ACGGGGGATA TGACCGATGG CTCTGGGGGT
    GCGGTGGAGC CGGGAGTTGA GCATGTGCCA AACCCACCAC CTTTACGAGG TGTAGAACAG
    CAGCCTCGGC TGATCCAGAG GATGGAGAGT GGCTACGAAA GTAGTGAGAG GAACAGCAAC
    AGCCCGATCA GCATGGACAT GCCCCTCAGC GACAACCCAA ACTCGAGTGC TCACAGGGAC
    TTTGGAAATA AGAGGCCTGT AACGTCTGGA CCATCTTGGC GCACTGTGCC CAAGTCCAAA
    AGTAGCAGTG CCATCCTGCA GGACCTCAAA TCACCCACCT GGAGCAGCGC TGCGCTAAGT
    TTGGGGGAAG AGCGTAGTGA GCTGGATGAA TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG
    CAACAGGAGC TGCAGAGGAA GAAGGAGAAG GAGAGAGAAG CGGCGCAGGG CTTTAATCCC
    AGACCCAGCA AATTCATGGA CCTGGATGAA CTTCAGCACC AGGGGAAGGG GGGCAGTTTT
    GAGCACTGCC TGGCGGAGGC TGACTCTCTC ATAGAGCAGT CGATGCGCCT GGAGCAGGCT
    GGGGACACAG CTACAGCCCT GGCGCTCTCC CACCACGCCG TGTCTAAACT AAGATTTTCC
    ATGCATGAGG GCAGTGCTAA CACTCATAGC AGGACAATGG CTGCTGACGC CAAGCTGCAA
    AAATGCATGA GGAGAGCGCG GGGCCTTCAG CAGCGCATGC AGCAACAGCA AGAACAGCCG
    CAGGAGCCCA GCCGCCCACA GGGGCCCATC AGTGAACAGC CTGGCCCGGT CCAAGTATTG
    TTGACAGACA ATCAGGAAGA GGTCTGGGAG ACCAATCCGG ATTCAGTGCT TGGCCCACCC
    TCTCCTCAAC ACATGAATTC ACCCTCCTCT TGTACAAACT CCCCATCTCA GCTCTCTAAC
    CGCCCATCGC TCAATCCAGG ATCTTCCCAG CCACATCCAC AAGAGTCTGA GGTATGCAGC
    AATTGGTCAA AACAGAAGGA GGAGGAATGG GGTGGCATCA GATTATCAGA CGTTCTCCAA
    GCATCAGGCA TTCGGGTCAT GCCCATACGG AAACACTTGG CCATATCTCT ACCCTCTTTG
    CCCCTCGACT TCTGGGGTGT ATTGGGAGAG GACGAAGAGC ACCCTCAAGC ATCGATCCCT
    CAAAATCCAA ATCCTCCTTT GTGGCAGGAC ATTGCAGATA GTCCAGAGAA GGTACGCCAA
    TCCAACACGT CTACCAGTCC AACACCTGCT ACAAATGAGG AAAAGGGGTC AATGCCCCGA
    CGAGTCCCTA TATGGCAGCC TCTATGTACA TCTTCCCATC CGTGCAACAA ACTGAGCCCG
    CCTGTTGCTG CCCGTCATTG GTCCTCTTGG AGTCTTGACC GTCCTGATGC TGTCATCACT
    CCCTATGACA CACCTCCTGA ACAGAACCTA CGGGCAGGAA GTGTCCTCTC CCAACACACA
    TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC ATGAGCACCA GGAAGAAGAA TATCTCTAAG
    CACTCGAGCT TCCAAAGCAT TGATCTGGAG CCCACTGAGG CTGAGGACCA GGAGCTCTCT
    GAACTGGACT CACTCTACCA GGCCAGTCTC CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG
    CGTGGTACCA GCTCACCTGC TGTTGGGAGA TCAGTACCTC CTGCTGCTCG AAAACTGCTC
    ACTGGAGTCG GACGCTCTCG AACCCCTACG GCTGAACTCG AGAGAACCGC TTATGGACCC
    CCAGGTTGTT ACAGCCCTAC TGGTTACATG AACATGCCTC TGTCGGTGGC CCATCAAGGG
    CTACAGTCAG AAGAGGACGA CCAGTATGAT GCGGATCACC TGCGCCGTAT TGCTCGCAGC
    CTGAGTGGAA CCGTCATTGG GCGACGCCAG AACCGTCTTG CTGTCTCCCG AAGCTTTCTC
    GGGGACTCTG GGGTTTCGTC ATGTCAAAGC TTCATGTACT CACCTAGTTG CCTTCAGTTT
    CCATTGTCTC ATTCAGACCT CCATCTGCTT CAACCCAGCT ATCTCCATCA TCATGCGTCA
    CCTTCACCCC TGTCTCCACA GCCTGGGCCT TGCAGAGAGG TTCCAGGATG TCTCCAGAGG
    TTCTCCGAGT TTCTGGCTGT GTCTGACTGG TGTCAAACTC TCTCCAGTGG GGAGCACTAC
    CGTCCATCAC TGAGGGTTCC TGGAGGACGT CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305022.1
    CDS440..4198
    Translation

    Target ORF information:

    RefSeq Version XM_009306747.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin specific peptidase 54b (usp54b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306747.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGATT CGTGTATCTT CTGTGCTCTG AAGAGCATCT TCGCTCAGTT CCAGTTCAGC 
    AGTGAGAAGG TGCTCCCATC GGATGCTCTG CGCAGCGCTC TGGCCAAAAC CTTCCAAGAC
    GAGCAGCGCT TTCAGCTGGG CATCATGGAT GACGCGGCCG AGTGTTTCGA GAACCTCCTG
    ATGCGGATCC ACTTCCACAT CTCAGACGAG AGCAAGGAGG ACATCTGTAC GGCCAAACAC
    TGCATCCCAC ATCAGAAGTT TGCTATGACA CTTTTCGAAC AGTGTGTTTG TAATAATTGT
    GGAGCCACAT CAGATCCTCT CCCCTTCATT CAGATGGTGC ACTATATCTC CACCACCTCT
    CTCTGCTGCA GTAACCAAGC GGTGCGAATG CTGGAGTGTA AAGAGAAGCC CACCCCTGAC
    ATGTTTGGAG AGCTTCTCCG CAACGCCAGC ACCATGGGTG ACCTGCGCAA CTGCCCGAGT
    AACTGTGGTG AGATGCTGCG GATTCGGCGA GTTCTAATGA ACTGCCCGGA GATCATCAGC
    ATCGGTCTTG TTTGGGATTC AGATCACTCT GATCTGGCTG AAGATGTCAT TCACAGCCTG
    GGCACATGCC TACGCTTGGG TGATCTCTTC TACCGTGTGA CGGATGAACG AGCTAAGCAG
    TCTGAGCTCT ATCTTGTGGG CATGGTTTGC TACTACGGCA AGCATTACTC CACCTTCTTC
    TTCCAGACCA AGATACGCAA ATGGATGTAC TTTGATGACG CACATGTGAA GGAGATTGGT
    CCCAAGTGGA AGGACGTGGT GTCGAGATGT ATAAAAGGCC ACTACCAACC TCTATTGCTG
    CTGTATGCTG ACCCGCGTGG CACACCCGTG TCTTCGCAAG ATATGCTGGC AGCACAGCTG
    GACCTGCACC ACAGCAGCAA GGCCGGCTAC GACAGCGAAG ACTCCGGACG GGAGCCCTCA
    ATTTCAAGTG ACACTCGCAC AGACTCTTCC ACAGACAGCT ACAGCTACAA GCACTCTCGA
    TCACACCATG AGTCCATGGC CTCCCATTTC TCTTCTGACT CTCAGGGAAC TGTGGTGTGT
    AATCAGGACA GCAGCGGTTC CCAGAACAGC ATGGACACTG CAGGGGAGGT TCTAGACCAG
    GCCACTCCTA TTTGCACGCC AAGACCCTCT TCTACTGGCC ATCTGCAGGA CTATAAAGAG
    ACTGTGAACA TGATCCACAG CAGACCTCTC TCATCGTCCT CTAGCTCAGG CGTGGGGGGT
    TCGGAAGCTG GAGCGCGAGC GAGAGACTGG GAGGACGAGA GCACCAGCAG CGAGTCCAAA
    TCCAGCTCCA GCGGAGGCCG CTATCGCCCC ACTTGGAGAC CCAGAAGAGA GGCCCTGAAC
    ATCGACAGCA TCTTCAGCCG GGAAAGACAT CGGCCTGTGG GATACACCAC GCTTGGACCT
    TCCCCTCTGC CGGAAGACCC CATGTCACCA GTGCACGGTG AGCTGGTCTC AGTGGCTGTG
    GCAGAGGGAT CAAACGGAGC AGCAGGAGAG ACGGGGGATA TGACCGATGG CTCTGGGGGT
    GCGGTGGAGC CGGGAGTTGA GCATGTGCCA AACCCACCAC CTTTACGAGG TGTAGAACAG
    CAGCCTCGGC TGATCCAGAG GATGGAGAGT GGCTACGAAA GTAGTGAGAG GAACAGCAAC
    AGCCCGATCA GCATGGACAT GCCCCTCAGC GACAACCCAA ACTCGAGTGC TCACAGGGAC
    TTTGGAAATA AGAGGCCTGT AACGTCTGGA CCATCTTGGC GCACTGTGCC CAAGTCCAAA
    AGTAGCAGTG CCATCCTGCA GGACCTCAAA TCACCCACCT GGAGCAGCGC TGCGCTAAGT
    TTGGGGGAAG AGCGTAGTGA GCTGGATGAA TTGCAGGAGG AAGTTGCTCG CAGGGCTAGG
    CAACAGGAGC TGCAGAGGAA GAAGGAGAAG GAGAGAGAAG CGGCGCAGGG CTTTAATCCC
    AGACCCAGCA AATTCATGGA CCTGGATGAA CTTCAGCACC AGGGGAAGGG GGGCAGTTTT
    GAGCACTGCC TGGCGGAGGC TGACTCTCTC ATAGAGCAGT CGATGCGCCT GGAGCAGGCT
    GGGGACACAG CTACAGCCCT GGCGCTCTCC CACCACGCCG TGTCTAAACT AAGATTTTCC
    ATGCATGAGG GCAGTGCTAA CACTCATAGC AGGACAATGG CTGCTGACGC CAAGCTGCAA
    AAATGCATGA GGAGAGCGCG GGGCCTTCAG CAGCGCATGC AGCAACAGCA AGAACAGCCG
    CAGGAGCCCA GCCGCCCACA GGGGCCCATC AGTGAACAGC CTGGCCCGGT CCAAGTATTG
    TTGACAGACA ATCAGGAAGA GGTCTGGGAG ACCAATCCGG ATTCAGTGCT TGGCCCACCC
    TCTCCTCAAC ACATGAATTC ACCCTCCTCT TGTACAAACT CCCCATCTCA GCTCTCTAAC
    CGCCCATCGC TCAATCCAGG ATCTTCCCAG CCACATCCAC AAGAGTCTGA GGTATGCAGC
    AATTGGTCAA AACAGAAGGA GGAGGAATGG GGTGGCATCA GATTATCAGA CGTTCTCCAA
    GCATCAGGCA TTCGGGTCAT GCCCATACGG AAACACTTGG CCATATCTCT ACCCTCTTTG
    CCCCTCGACT TCTGGGGTGT ATTGGGAGAG GACGAAGAGC ACCCTCAAGC ATCGATCCCT
    CAAAATCCAA ATCCTCCTTT GTGGCAGGAC ATTGCAGATA GTCCAGAGAA GGTACGCCAA
    TCCAACACGT CTACCAGTCC AACACCTGCT ACAAATGAGG AAAAGGGGTC AATGCCCCGA
    CGAGTCCCTA TATGGCAGCC TCTATGTACA TCTTCCCATC CGTGCAACAA ACTGAGCCCG
    CCTGTTGCTG CCCGTCATTG GTCCTCTTGG AGTCTTGACC GTCCTGATGC TGTCATCACT
    CCCTATGACA CACCTCCTGA ACAGAACCTA CGGGCAGGAA GTGTCCTCTC CCAACACACA
    TCTGGAAGAC ACCTTCCTCC TCGCAGTCCC ATGAGCACCA GGAAGAAGAA TATCTCTAAG
    CACTCGAGCT TCCAAAGCAT TGATCTGGAG CCCACTGAGG CTGAGGACCA GGAGCTCTCT
    GAACTGGACT CACTCTACCA GGCCAGTCTC CAGGCTGGCC CGGCTCCTGG TCGAGCCTTG
    CGTGGTACCA GCTCACCTGC TGTTGGGAGA TCAGTACCTC CTGCTGCTCG AAAACTGCTC
    ACTGGAGTCG GACGCTCTCG AACCCCTACG GCTGAACTCG AGAGAACCGC TTATGGACCC
    CCAGGTTGTT ACAGCCCTAC TGGTTACATG AACATGCCTC TGTCGGTGGC CCATCAAGGG
    CTACAGTCAG AAGAGGACGA CCAGTATGAT GCGGATCACC TGCGCCGTAT TGCTCGCAGC
    CTGAGTGGAA CCGTCATTGG GCGACGCCAG AACCGTCTTG CTGTCTCCCG AAGCTTTCTC
    GGGGACTCTG GGGTTTCGTC ATGTCAAAGC TTCATGTACT CACCTAGTTG CCTTCAGTTT
    CCATTGTCTC ATTCAGACCT CCATCTGCTT CAACCCAGCT ATCTCCATCA TCATGCGTCA
    CCTTCACCCC TGTCTCCACA GCCTGGGCCT TGCAGAGAGG TTCCAGGATG TCTCCAGAGG
    TTCTCCGAGT TTCTGGCTGT GTCTGACTGG TGTCAAACTC TCTCCAGTGG GGAGCACTAC
    CGTCCATCAC TGAGGGTTCC TGGAGGACGT CCTGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.