USP54 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP54
Entrez Gene ID	159195
Full Name	ubiquitin specific peptidase 54
Synonyms	C10orf29,bA137L10.3,bA137L10.4
General protein information	Preferred Names ubiquitin specific peptidase 54 Names inactive ubiquitin carboxyl-terminal hydrolase 54 inactive ubiquitin-specific peptidase 54 ubiquitin specific protease 54 ubiquitin specific proteinase 54
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017015769.1	XP_016871258.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015761.1	XP_016871250.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447842.1	XP_024303610.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447850.1	XP_024303618.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
NM_001320441.1	NP_001307370.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 3
XM_017015759.1	XP_016871248.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015781.1	XP_016871270.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
XM_017015782.1	XP_016871271.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X11
XM_017015764.1	XP_016871253.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015767.1	XP_016871256.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015770.1	XP_016871259.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015779.1	XP_016871268.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
XM_017015776.1	XP_016871265.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
XM_024447835.1	XP_024303603.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447836.1	XP_024303604.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447837.1	XP_024303605.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447838.1	XP_024303606.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447841.1	XP_024303609.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015774.1	XP_016871263.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_024447851.1	XP_024303619.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X10
XM_017015780.1	XP_016871269.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X8
XM_017015768.1	XP_016871257.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015773.1	XP_016871262.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
NM_152586.3	NP_689799.3	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 1
NM_001350995.2	NP_001337924.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 4
NM_001320441.2	NP_001307370.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 3
XM_024447833.1	XP_024303601.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447845.1	XP_024303613.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_011539368.2	XP_011537670.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
XM_017015782.2	XP_016871271.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X8
XM_017015783.1	XP_016871272.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
XM_005269582.2	XP_005269639.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X12
XM_017015765.1	XP_016871254.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015762.1	XP_016871251.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015785.1	XP_016871274.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X13
NM_001320437.2	NP_001307366.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 2
XM_024447832.1	XP_024303600.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447834.1	XP_024303602.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447843.1	XP_024303611.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447844.1	XP_024303612.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
NM_001350995.1	NP_001337924.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 4
XM_017015760.1	XP_016871249.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015771.1	XP_016871260.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_017015772.1	XP_016871261.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_017015778.1	XP_016871267.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
XM_017015763.1	XP_016871252.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_017015766.1	XP_016871255.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
NM_001320437.1	NP_001307366.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 2
XM_024447839.1	XP_024303607.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447840.1	XP_024303608.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_024447846.1	XP_024303614.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_017015775.1	XP_016871264.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_024447847.1	XP_024303615.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_017015777.1	XP_016871266.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_024447848.1	XP_024303616.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
XM_024447849.1	XP_024303617.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
XM_017015784.1	XP_016871273.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
XM_024447852.1	XP_024303620.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X11

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP54	NP_689799.3
Mus musculus (house mouse)	Usp54	NP_084456.2
Canis lupus familiaris (dog)	USP54	XP_005619078.1
Bos taurus (cattle)	USP54	XP_005226561.1
Gallus gallus (chicken)	USP54	XP_004942143.1
Danio rerio (zebrafish)	usp54a	NP_001188495.1
	LOC706859	XP_002808545.1
Rattus norvegicus (Norway rat)	Usp54	NP_001008863.2
Pan troglodytes (chimpanzee)	USP54	XP_003312678.1
Danio rerio (zebrafish)	LOC100535699	XP_003199659.2
Xenopus tropicalis (tropical clawed frog)	usp54	XP_002935811.2

Gene Ontology
Bibliography

Related articles in PubMed

The deubiquitinase USP54 is overexpressed in colorectal cancer stem cells and promotes intestinal tumorigenesis.
Fraile JM, Campos-Iglesias D, Rodr?guez F, Espa?ol Y, Freije JM
Oncotarget7(46)74427-74434(2016 Nov)

Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases.
Quesada V, D?az-Perales A, Guti?rrez-Fern?ndez A, Garabaya C, Cal S, L?pez-Ot?n C
Biochemical and biophysical research communications314(1)54-62(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.

The following USP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06851	NM_152586.3 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu06851	XM_017015774.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu06851	XM_024447847.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu06851	XM_017015776.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu06851	XM_017015775.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu06851	XM_017015777.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu101837	NM_001320441.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu101876	NM_001320437.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu102473	NM_001350995.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu102473	XM_024447852.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu44064	XM_024447848.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu44067	XM_024447851.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu77040	XM_024447846.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu77041	XM_024447849.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu77042	XM_024447850.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91145	XM_017015766.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015764.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015768.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015770.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015763.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015761.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015765.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015769.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015767.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015759.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015762.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91145	XM_017015760.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91146	XM_017015772.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91147	XM_017015773.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91148	XM_017015778.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91149	XM_017015779.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91150	XM_017015780.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91151	XM_017015781.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91152	XM_017015782.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91153	XM_017015783.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91153	XM_017015784.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu91154	XM_017015785.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu44060	XM_024447844.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_017015771.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447840.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447842.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447837.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447833.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447835.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447845.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447841.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447843.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447839.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447838.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447836.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447834.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44060	XM_024447832.1 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44068	XM_005269582.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu77043	XM_011539368.2 Latest version!	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu101837	NM_001320441.1	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu91152	XM_017015782.1	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu102473	NM_001350995.1	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu101876	NM_001320437.1	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP54 ( NM_152586.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689799.3 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	NM_152586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN288586.1, AK127267.1, BX649087.1, AK126497.1 and BC064967.1. On Jan 23, 2007 this sequence version replaced NM_152586.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689799.3
CDS	18..5072
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	NM_152586.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152586.3

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015776.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871265.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.

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CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	XM_017015776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871265.1
CDS	2642..7696
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015776.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015776.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015774.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871263.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X16, mRNA.

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CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	XM_017015774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871263.1
CDS	2259..7313
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015774.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015774.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015775.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871264.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X17, mRNA.

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CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	XM_017015775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871264.1
CDS	2137..7191
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015775.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015775.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447847.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303615.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.

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CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	XM_024447847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303615.1
CDS	636..5690
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447847.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447847.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015777.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871266.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X19, mRNA.

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CloneID	OHu06851
Clone ID Related Accession (Same CDS sequence)	XM_017015776.1 , XM_017015774.1 , NM_152586.3 , XM_017015775.1 , XM_024447847.1 , XM_017015777.1
Accession Version	XM_017015777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871266.1
CDS	2301..7355
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015777.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015777.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGGGAGGAGT GACGGCTTTG AGAGGTCCCT GCAAGAGGCA 
GAGTCAGTGT TTGAAGAGTC ACTACATCTG GAACAGAAAG GAGACTGTGC TGCAGCTTTG 
GCTCTCTGTA ATGAAGCTAT CTCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001320441.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307370.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101837
Clone ID Related Accession (Same CDS sequence)	NM_001320441.1 , NM_001320441.2
Accession Version	NM_001320441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC322498.1, AK302598.1 and AL359074.12. Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK302598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GTATTGTTTG GCCTTTGAAC AGCAACATTG TCTCTTGCAG 
CAATGTGAGC CACCTACTTG GGGCTTTCAG GCATGGCTGA GACAAGTGGA GGGATGGCCT 
TTGCTATTAT CTCTATGTGC TCTTTCAACT TCCTATAACA ACAGCACCCT CTGCTGGAAG 
AACAGAGGCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307370.1
CDS	655..2889
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRYCLAFEQQHCLLQQCEPPTWGFQAWLRQVEGWPLLLSLCALSTSYNNSTLCWKNRGT

Target ORF information:

RefSeq Version	NM_001320441.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320441.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GTATTGTTTG GCCTTTGAAC AGCAACATTG TCTCTTGCAG 
CAATGTGAGC CACCTACTTG GGGCTTTCAG GCATGGCTGA GACAAGTGGA GGGATGGCCT 
TTGCTATTAT CTCTATGTGC TCTTTCAACT TCCTATAACA ACAGCACCCT CTGCTGGAAG 
AACAGAGGCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001320441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307370.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.

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CloneID	OHu101837
Clone ID Related Accession (Same CDS sequence)	NM_001320441.1 , NM_001320441.2
Accession Version	NM_001320441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC322498.1, AK302598.1 and AL359074.12. On Jun 2, 2019 this sequence version replaced NM_001320441.1. Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK302598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GTATTGTTTG GCCTTTGAAC AGCAACATTG TCTCTTGCAG 
CAATGTGAGC CACCTACTTG GGGCTTTCAG GCATGGCTGA GACAAGTGGA GGGATGGCCT 
TTGCTATTAT CTCTATGTGC TCTTTCAACT TCCTATAACA ACAGCACCCT CTGCTGGAAG 
AACAGAGGCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307370.1
CDS	647..2881
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRYCLAFEQQHCLLQQCEPPTWGFQAWLRQVEGWPLLLSLCALSTSYNNSTLCWKNRGT

Target ORF information:

RefSeq Version	NM_001320441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320441.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GTATTGTTTG GCCTTTGAAC AGCAACATTG TCTCTTGCAG 
CAATGTGAGC CACCTACTTG GGGCTTTCAG GCATGGCTGA GACAAGTGGA GGGATGGCCT 
TTGCTATTAT CTCTATGTGC TCTTTCAACT TCCTATAACA ACAGCACCCT CTGCTGGAAG 
AACAGAGGCA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001320437.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307366.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.

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CloneID	OHu101876
Clone ID Related Accession (Same CDS sequence)	NM_001320437.2 , NM_001320437.1
Accession Version	NM_001320437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC322498.1, DA327288.1, AK302598.1, AL359074.12, AK127267.1 and BC064967.1. On Jun 2, 2019 this sequence version replaced NM_001320437.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGCTACA CCTTGCCGAG GCCTCAGCAG GGAGTGTGGG 
GAGGATGAGC AGTACAGTGC AGAGAATTTA CGTCGCATCT CACGCAGTCT CAGTGGCACC 
GTTGTCTCAG AGAGGGAGGA AGCTCCGGTT TCTTCCCACA GTTTTGATTC ATCAAACGTG 
AGGAAGCCTT TGGAAACCGG GCACCGTTGT TCCAGCTCCT CTTCCCTCCC TGTCATCCAT 
GACCCTTCTG TGTTTCTCCT CGGTCCCCAA CTCTACCTTC CCCAACCACA GTTCCTGTCC 
CCAGATGTCC TGATGCCCAC CATGGCAGGG GAGCCCAATA GACTCCCAGG AACTTCAAGG 
AGTGTCCAGC AGTTTCTGGC TATGTGTGAC AGGGGTGAAA CTTCCCAAGG GGCCAAGTAC 
ACAGGAAGGA CTTTGAACTA CCAGAGCCTC CCCCATCGCT CCAGAACAGA CAACTCCTGG 
GCACCCTGGT CAGAGACCAA CCAGCATATT GGGACCAGAT TCCTGACTAC TCCAGGGTGC 
AATCCTCAAC TAACCTACAC TGCCACACTA CCAGAAAGAA GCAAGGGCCT TCAGGTTCCT 
CACACTCAGT CCTGGAGTGA TCTTTTCCAT TCACCCTCCC ACCCTCCCAT TGTTCATCCT 
GTGTACCCAC CATCTAGCAG TCTTCATGTA CCCCTGAGGT CAGCTTGGAA TTCAGATCCT 
GTTCCAGGGT CCCGAACCCC TGGTCCTCGA AGAGTAGATA TGCCCCCAGA TGATGACTGG 
AGGCAAAGCA GTTATGCCTC CCACTCTGGA CACAGGAGAA CAGTGGGAGA GGGGTTTCTG 
TTTGTTCTAT CAGATGCTCC CAGAAGAGAG CAGATCAGGG CTAGAGTCCT GCAGCACAGT 
CAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307366.1
CDS	83..4951
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	NM_001320437.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320437.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGCTACA CCTTGCCGAG GCCTCAGCAG GGAGTGTGGG 
GAGGATGAGC AGTACAGTGC AGAGAATTTA CGTCGCATCT CACGCAGTCT CAGTGGCACC 
GTTGTCTCAG AGAGGGAGGA AGCTCCGGTT TCTTCCCACA GTTTTGATTC ATCAAACGTG 
AGGAAGCCTT TGGAAACCGG GCACCGTTGT TCCAGCTCCT CTTCCCTCCC TGTCATCCAT 
GACCCTTCTG TGTTTCTCCT CGGTCCCCAA CTCTACCTTC CCCAACCACA GTTCCTGTCC 
CCAGATGTCC TGATGCCCAC CATGGCAGGG GAGCCCAATA GACTCCCAGG AACTTCAAGG 
AGTGTCCAGC AGTTTCTGGC TATGTGTGAC AGGGGTGAAA CTTCCCAAGG GGCCAAGTAC 
ACAGGAAGGA CTTTGAACTA CCAGAGCCTC CCCCATCGCT CCAGAACAGA CAACTCCTGG 
GCACCCTGGT CAGAGACCAA CCAGCATATT GGGACCAGAT TCCTGACTAC TCCAGGGTGC 
AATCCTCAAC TAACCTACAC TGCCACACTA CCAGAAAGAA GCAAGGGCCT TCAGGTTCCT 
CACACTCAGT CCTGGAGTGA TCTTTTCCAT TCACCCTCCC ACCCTCCCAT TGTTCATCCT 
GTGTACCCAC CATCTAGCAG TCTTCATGTA CCCCTGAGGT CAGCTTGGAA TTCAGATCCT 
GTTCCAGGGT CCCGAACCCC TGGTCCTCGA AGAGTAGATA TGCCCCCAGA TGATGACTGG 
AGGCAAAGCA GTTATGCCTC CCACTCTGGA CACAGGAGAA CAGTGGGAGA GGGGTTTCTG 
TTTGTTCTAT CAGATGCTCC CAGAAGAGAG CAGATCAGGG CTAGAGTCCT GCAGCACAGT 
CAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001320437.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307366.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.

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CloneID	OHu101876
Clone ID Related Accession (Same CDS sequence)	NM_001320437.2 , NM_001320437.1
Accession Version	NM_001320437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC322498.1, DA327288.1, AK302598.1, AL359074.12, AK127267.1 and BC064967.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGCTACA CCTTGCCGAG GCCTCAGCAG GGAGTGTGGG 
GAGGATGAGC AGTACAGTGC AGAGAATTTA CGTCGCATCT CACGCAGTCT CAGTGGCACC 
GTTGTCTCAG AGAGGGAGGA AGCTCCGGTT TCTTCCCACA GTTTTGATTC ATCAAACGTG 
AGGAAGCCTT TGGAAACCGG GCACCGTTGT TCCAGCTCCT CTTCCCTCCC TGTCATCCAT 
GACCCTTCTG TGTTTCTCCT CGGTCCCCAA CTCTACCTTC CCCAACCACA GTTCCTGTCC 
CCAGATGTCC TGATGCCCAC CATGGCAGGG GAGCCCAATA GACTCCCAGG AACTTCAAGG 
AGTGTCCAGC AGTTTCTGGC TATGTGTGAC AGGGGTGAAA CTTCCCAAGG GGCCAAGTAC 
ACAGGAAGGA CTTTGAACTA CCAGAGCCTC CCCCATCGCT CCAGAACAGA CAACTCCTGG 
GCACCCTGGT CAGAGACCAA CCAGCATATT GGGACCAGAT TCCTGACTAC TCCAGGGTGC 
AATCCTCAAC TAACCTACAC TGCCACACTA CCAGAAAGAA GCAAGGGCCT TCAGGTTCCT 
CACACTCAGT CCTGGAGTGA TCTTTTCCAT TCACCCTCCC ACCCTCCCAT TGTTCATCCT 
GTGTACCCAC CATCTAGCAG TCTTCATGTA CCCCTGAGGT CAGCTTGGAA TTCAGATCCT 
GTTCCAGGGT CCCGAACCCC TGGTCCTCGA AGAGTAGATA TGCCCCCAGA TGATGACTGG 
AGGCAAAGCA GTTATGCCTC CCACTCTGGA CACAGGAGAA CAGTGGGAGA GGGGTTTCTG 
TTTGTTCTAT CAGATGCTCC CAGAAGAGAG CAGATCAGGG CTAGAGTCCT GCAGCACAGT 
CAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307366.1
CDS	91..4959
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	NM_001320437.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320437.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGCTACA CCTTGCCGAG GCCTCAGCAG GGAGTGTGGG 
GAGGATGAGC AGTACAGTGC AGAGAATTTA CGTCGCATCT CACGCAGTCT CAGTGGCACC 
GTTGTCTCAG AGAGGGAGGA AGCTCCGGTT TCTTCCCACA GTTTTGATTC ATCAAACGTG 
AGGAAGCCTT TGGAAACCGG GCACCGTTGT TCCAGCTCCT CTTCCCTCCC TGTCATCCAT 
GACCCTTCTG TGTTTCTCCT CGGTCCCCAA CTCTACCTTC CCCAACCACA GTTCCTGTCC 
CCAGATGTCC TGATGCCCAC CATGGCAGGG GAGCCCAATA GACTCCCAGG AACTTCAAGG 
AGTGTCCAGC AGTTTCTGGC TATGTGTGAC AGGGGTGAAA CTTCCCAAGG GGCCAAGTAC 
ACAGGAAGGA CTTTGAACTA CCAGAGCCTC CCCCATCGCT CCAGAACAGA CAACTCCTGG 
GCACCCTGGT CAGAGACCAA CCAGCATATT GGGACCAGAT TCCTGACTAC TCCAGGGTGC 
AATCCTCAAC TAACCTACAC TGCCACACTA CCAGAAAGAA GCAAGGGCCT TCAGGTTCCT 
CACACTCAGT CCTGGAGTGA TCTTTTCCAT TCACCCTCCC ACCCTCCCAT TGTTCATCCT 
GTGTACCCAC CATCTAGCAG TCTTCATGTA CCCCTGAGGT CAGCTTGGAA TTCAGATCCT 
GTTCCAGGGT CCCGAACCCC TGGTCCTCGA AGAGTAGATA TGCCCCCAGA TGATGACTGG 
AGGCAAAGCA GTTATGCCTC CCACTCTGGA CACAGGAGAA CAGTGGGAGA GGGGTTTCTG 
TTTGTTCTAT CAGATGCTCC CAGAAGAGAG CAGATCAGGG CTAGAGTCCT GCAGCACAGT 
CAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001350995.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337924.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.

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CloneID	OHu102473
Clone ID Related Accession (Same CDS sequence)	NM_001350995.2 , NM_001350995.1 , XM_024447852.1
Accession Version	NM_001350995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC073389.10, AK302598.1, AL359074.12, AK127267.1, BC026037.1 and BC064967.1. On Jun 2, 2019 this sequence version replaced NM_001350995.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.215194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337924.1
CDS	289..5052
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	NM_001350995.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350995.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( NM_001350995.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337924.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.

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CloneID	OHu102473
Clone ID Related Accession (Same CDS sequence)	NM_001350995.2 , NM_001350995.1 , XM_024447852.1
Accession Version	NM_001350995.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC073389.10, AK302598.1, AL359074.12, AK127267.1, BC026037.1 and BC064967.1. On Apr 29, 2017 this sequence version replaced XM_017015786.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.215194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337924.1
CDS	367..5130
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	NM_001350995.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350995.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447852.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303620.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu102473
Clone ID Related Accession (Same CDS sequence)	NM_001350995.2 , NM_001350995.1 , XM_024447852.1
Accession Version	XM_024447852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303620.1
CDS	3267..8030
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447852.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447852.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447848.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303616.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu44064
Clone ID Related Accession (Same CDS sequence)	XM_024447848.1
Accession Version	XM_024447848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303616.1
CDS	3267..8267
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447848.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447848.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447851.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303619.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X27, mRNA.

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CloneID	OHu44067
Clone ID Related Accession (Same CDS sequence)	XM_024447851.1
Accession Version	XM_024447851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGATT CATCAAACGT GAGGAAGCCT 
TTGGAAACCG GGCACCGTTG TTCCAGCTCC TCTTCCCTCC CTGTCATCCA TGACCCTTCT 
GTGTTTCTCC TCGGTCCCCA ACTCTACCTT CCCCAACCAC AGTTCCTGTC CCCAGATGTC 
CTGATGCCCA CCATGGCAGG GGAGCCCAAT AGACTCCCAG GAACTTCAAG GAGTGTCCAG 
CAGTTTCTGG CTATGTGTGA CAGGGGTGAA ACTTCCCAAG GGGCCAAGTA CACAGGAAGG 
ACTTTGAACT ACCAGAGCCT CCCCCATCGC TCCAGAACAG ACAACTCCTG GGCACCCTGG 
TCAGAGACCA ACCAGCATAT TGGGACCAGA TTCCTGACTA CTCCAGGGTG CAATCCTCAA 
CTAACCTACA CTGCCACACT ACCAGAAAGA AGCAAGGGCC TTCAGGTTCC TCACACTCAG 
TCCTGGAGTG ATCTTTTCCA TTCACCCTCC CACCCTCCCA TTGTTCATCC TGTGTACCCA 
CCATCTAGCA GTCTTCATGT ACCCCTGAGG TCAGCTTGGA ATTCAGATCC TGTTCCAGGG 
TCCCGAACCC CTGGTCCTCG AAGAGTAGAT ATGCCCCCAG ATGATGACTG GAGGCAAAGC 
AGTTATGCCT CCCACTCTGG ACACAGGAGA ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA 
TCAGATGCTC CCAGAAGAGA GCAGATCAGG GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303619.1
CDS	3267..8126
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447851.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447851.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGATT CATCAAACGT GAGGAAGCCT 
TTGGAAACCG GGCACCGTTG TTCCAGCTCC TCTTCCCTCC CTGTCATCCA TGACCCTTCT 
GTGTTTCTCC TCGGTCCCCA ACTCTACCTT CCCCAACCAC AGTTCCTGTC CCCAGATGTC 
CTGATGCCCA CCATGGCAGG GGAGCCCAAT AGACTCCCAG GAACTTCAAG GAGTGTCCAG 
CAGTTTCTGG CTATGTGTGA CAGGGGTGAA ACTTCCCAAG GGGCCAAGTA CACAGGAAGG 
ACTTTGAACT ACCAGAGCCT CCCCCATCGC TCCAGAACAG ACAACTCCTG GGCACCCTGG 
TCAGAGACCA ACCAGCATAT TGGGACCAGA TTCCTGACTA CTCCAGGGTG CAATCCTCAA 
CTAACCTACA CTGCCACACT ACCAGAAAGA AGCAAGGGCC TTCAGGTTCC TCACACTCAG 
TCCTGGAGTG ATCTTTTCCA TTCACCCTCC CACCCTCCCA TTGTTCATCC TGTGTACCCA 
CCATCTAGCA GTCTTCATGT ACCCCTGAGG TCAGCTTGGA ATTCAGATCC TGTTCCAGGG 
TCCCGAACCC CTGGTCCTCG AAGAGTAGAT ATGCCCCCAG ATGATGACTG GAGGCAAAGC 
AGTTATGCCT CCCACTCTGG ACACAGGAGA ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA 
TCAGATGCTC CCAGAAGAGA GCAGATCAGG GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447846.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303614.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.

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CloneID	OHu77040
Clone ID Related Accession (Same CDS sequence)	XM_024447846.1
Accession Version	XM_024447846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGCTACACCT TGCCGAGGCC TCAGCAGGGA GTGTGGGGAG 
GATGAGCAGT ACAGTGCAGA GAATTTACGT CGCATCTCAC GCAGTCTCAG TGGCACCGTT 
GTCTCAGAGA GGGAGGAAGC TCCGGTTTCT TCCCACAGTT TTGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303614.1
CDS	3267..8372
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447846.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGCTACACCT TGCCGAGGCC TCAGCAGGGA GTGTGGGGAG 
GATGAGCAGT ACAGTGCAGA GAATTTACGT CGCATCTCAC GCAGTCTCAG TGGCACCGTT 
GTCTCAGAGA GGGAGGAAGC TCCGGTTTCT TCCCACAGTT TTGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447849.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303617.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.

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CloneID	OHu77041
Clone ID Related Accession (Same CDS sequence)	XM_024447849.1
Accession Version	XM_024447849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGATT CATCAAACGT GAGGAAGCCT 
TTGGAAACCG GGCACCGTTG TTCCAGCTCC TCTTCCCTCC CTGTCATCCA TGACCCTTCT 
GTGTTTCTCC TCGGTCCCCA ACTCTACCTT CCCCAACCAC AGTTCCTGTC CCCAGATGTC 
CTGATGCCCA CCATGGCAGG GGAGCCCAAT AGACTCCCAG GAACTTCAAG GAGTGTCCAG 
CAGTTTCTGG CTATGTGTGA CAGGGGTGAA ACTTCCCAAG GGGCCAAGTA CACAGGAAGG 
ACTTTGAACT ACCAGAGCCT CCCCCATCGC TCCAGAACAG ACAACTCCTG GGCACCCTGG 
TCAGAGACCA ACCAGCATAT TGGGACCAGA TTCCTGACTA CTCCAGGGTG CAATCCTCAA 
CTAACCTACA CTGCCACACT ACCAGAAAGA AGCAAGGGCC TTCAGGTTCC TCACACTCAG 
TCCTGGAGTG ATCTTTTCCA TTCACCCTCC CACCCTCCCA TTGTTCATCC TGTGTACCCA 
CCATCTAGCA GTCTTCATGT ACCCCTGAGG TCAGCTTGGA ATTCAGATCC TGTTCCAGGG 
TCCCGAACCC CTGGTCCTCG AAGAGTAGAT ATGCCCCCAG ATGATGACTG GAGGCAAAGC 
AGTTATGCCT CCCACTCTGG ACACAGGAGA ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA 
TCAGATGCTC CCAGAAGAGA GCAGATCAGG GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303617.1
CDS	3267..8246
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447849.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447849.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGATT CATCAAACGT GAGGAAGCCT 
TTGGAAACCG GGCACCGTTG TTCCAGCTCC TCTTCCCTCC CTGTCATCCA TGACCCTTCT 
GTGTTTCTCC TCGGTCCCCA ACTCTACCTT CCCCAACCAC AGTTCCTGTC CCCAGATGTC 
CTGATGCCCA CCATGGCAGG GGAGCCCAAT AGACTCCCAG GAACTTCAAG GAGTGTCCAG 
CAGTTTCTGG CTATGTGTGA CAGGGGTGAA ACTTCCCAAG GGGCCAAGTA CACAGGAAGG 
ACTTTGAACT ACCAGAGCCT CCCCCATCGC TCCAGAACAG ACAACTCCTG GGCACCCTGG 
TCAGAGACCA ACCAGCATAT TGGGACCAGA TTCCTGACTA CTCCAGGGTG CAATCCTCAA 
CTAACCTACA CTGCCACACT ACCAGAAAGA AGCAAGGGCC TTCAGGTTCC TCACACTCAG 
TCCTGGAGTG ATCTTTTCCA TTCACCCTCC CACCCTCCCA TTGTTCATCC TGTGTACCCA 
CCATCTAGCA GTCTTCATGT ACCCCTGAGG TCAGCTTGGA ATTCAGATCC TGTTCCAGGG 
TCCCGAACCC CTGGTCCTCG AAGAGTAGAT ATGCCCCCAG ATGATGACTG GAGGCAAAGC 
AGTTATGCCT CCCACTCTGG ACACAGGAGA ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA 
TCAGATGCTC CCAGAAGAGA GCAGATCAGG GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447850.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303618.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.

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CloneID	OHu77042
Clone ID Related Accession (Same CDS sequence)	XM_024447850.1
Accession Version	XM_024447850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGATTCATCA AACGTGAGGA AGCCTTTGGA AACCGGGCAC 
CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC ATCCATGACC CTTCTGTGTT TCTCCTCGGT 
CCCCAACTCT ACCTTCCCCA ACCACAGTTC CTGTCCCCAG ATGTCCTGAT GCCCACCATG 
GCAGGGGAGC CCAATAGACT CCCAGGAACT TCAAGGAGTG TCCAGCAGTT TCTGGCTATG 
TGTGACAGGG GTGAAACTTC CCAAGGGGCC AAGTACACAG GAAGGACTTT GAACTACCAG 
AGCCTCCCCC ATCGCTCCAG AACAGACAAC TCCTGGGCAC CCTGGTCAGA GACCAACCAG 
CATATTGGGA CCAGATTCCT GACTACTCCA GGGTGCAATC CTCAACTAAC CTACACTGCC 
ACACTACCAG AAAGAAGCAA GGGCCTTCAG GTTCCTCACA CTCAGTCCTG GAGTGATCTT 
TTCCATTCAC CCTCCCACCC TCCCATTGTT CATCCTGTGT ACCCACCATC TAGCAGTCTT 
CATGTACCCC TGAGGTCAGC TTGGAATTCA GATCCTGTTC CAGGGTCCCG AACCCCTGGT 
CCTCGAAGAG TAGATATGCC CCCAGATGAT GACTGGAGGC AAAGCAGTTA TGCCTCCCAC 
TCTGGACACA GGAGAACAGT GGGAGAGGGG TTTCTGTTTG TTCTATCAGA TGCTCCCAGA 
AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303618.1
CDS	3267..8231
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447850.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447850.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGATTCATCA AACGTGAGGA AGCCTTTGGA AACCGGGCAC 
CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC ATCCATGACC CTTCTGTGTT TCTCCTCGGT 
CCCCAACTCT ACCTTCCCCA ACCACAGTTC CTGTCCCCAG ATGTCCTGAT GCCCACCATG 
GCAGGGGAGC CCAATAGACT CCCAGGAACT TCAAGGAGTG TCCAGCAGTT TCTGGCTATG 
TGTGACAGGG GTGAAACTTC CCAAGGGGCC AAGTACACAG GAAGGACTTT GAACTACCAG 
AGCCTCCCCC ATCGCTCCAG AACAGACAAC TCCTGGGCAC CCTGGTCAGA GACCAACCAG 
CATATTGGGA CCAGATTCCT GACTACTCCA GGGTGCAATC CTCAACTAAC CTACACTGCC 
ACACTACCAG AAAGAAGCAA GGGCCTTCAG GTTCCTCACA CTCAGTCCTG GAGTGATCTT 
TTCCATTCAC CCTCCCACCC TCCCATTGTT CATCCTGTGT ACCCACCATC TAGCAGTCTT 
CATGTACCCC TGAGGTCAGC TTGGAATTCA GATCCTGTTC CAGGGTCCCG AACCCCTGGT 
CCTCGAAGAG TAGATATGCC CCCAGATGAT GACTGGAGGC AAAGCAGTTA TGCCTCCCAC 
TCTGGACACA GGAGAACAGT GGGAGAGGGG TTTCTGTTTG TTCTATCAGA TGCTCCCAGA 
AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015769.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871258.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871258.1
CDS	83..5209
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015769.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015769.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015761.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871250.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871250.1
CDS	2259..7385
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015761.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015761.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015759.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871248.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871248.1
CDS	2519..7645
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015759.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015759.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015764.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871253.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871253.1
CDS	786..5912
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015764.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015764.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015767.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871256.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871256.1
CDS	2304..7430
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015767.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015767.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015770.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871259.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871259.1
CDS	744..5870
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015770.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015770.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015768.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871257.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871257.1
CDS	475..5601
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015768.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015768.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015765.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871254.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871254.1
CDS	1039..6165
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015765.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015762.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871251.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871251.1
CDS	1429..6555
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015762.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015762.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015760.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871249.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871249.1
CDS	1421..6547
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015760.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015760.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015763.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871252.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871252.1
CDS	723..5849
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015763.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015763.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015766.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871255.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

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CloneID	OHu91145
Clone ID Related Accession (Same CDS sequence)	XM_017015769.1 , XM_017015761.1 , XM_017015759.1 , XM_017015764.1 , XM_017015767.1 , XM_017015770.1 , XM_017015768.1 , XM_017015765.1 , XM_017015762.1 , XM_017015760.1 , XM_017015763.1 , XM_017015766.1
Accession Version	XM_017015766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871255.1
CDS	2645..7771
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015766.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015766.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015772.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871261.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.

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CloneID	OHu91146
Clone ID Related Accession (Same CDS sequence)	XM_017015772.1
Accession Version	XM_017015772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGCT ACACCTTGCC GAGGCCTCAG CAGGGAGTGT 
GGGGAGGATG AGCAGTACAG TGCAGAGAAT TTACGTCGCA TCTCACGCAG TCTCAGTGGC 
ACCGTTGTCT CAGAGAGGGA GGAAGCTCCG GTTTCTTCCC ACAGTTTTGA TTCATCAAAC 
GTGAGGAAGC CTTTGGAAAC CGGGCACCGT TGTTCCAGCT CCTCTTCCCT CCCTGTCATC 
CATGACCCTT CTGTGTTTCT CCTCGGTCCC CAACTCTACC TTCCCCAACC ACAGTTCCTG 
TCCCCAGATG TCCTGATGCC CACCATGGCA GGGGAGCCCA ATAGACTCCC AGGAACTTCA 
AGGAGTGTCC AGCAGTTTCT GGCTATGTGT GACAGGGGTG AAACTTCCCA AGGGGCCAAG 
TACACAGGAA GGACTTTGAA CTACCAGAGC CTCCCCCATC GCTCCAGAAC AGACAACTCC 
TGGGCACCCT GGTCAGAGAC CAACCAGCAT ATTGGGACCA GATTCCTGAC TACTCCAGGG 
TGCAATCCTC AACTAACCTA CACTGCCACA CTACCAGAAA GAAGCAAGGG CCTTCAGGTT 
CCTCACACTC AGTCCTGGAG TGATCTTTTC CATTCACCCT CCCACCCTCC CATTGTTCAT 
CCTGTGTACC CACCATCTAG CAGTCTTCAT GTACCCCTGA GGTCAGCTTG GAATTCAGAT 
CCTGTTCCAG GGTCCCGAAC CCCTGGTCCT CGAAGAGTAG ATATGCCCCC AGATGATGAC 
TGGAGGCAAA GCAGTTATGC CTCCCACTCT GGACACAGGA GAACAGTGGG AGAGGGGTTT 
CTGTTTGTTC TATCAGATGC TCCCAGAAGA GAGCAGATCA GGGCTAGAGT CCTGCAGCAC 
AGTCAATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871261.1
CDS	3267..8378
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015772.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGCT ACACCTTGCC GAGGCCTCAG CAGGGAGTGT 
GGGGAGGATG AGCAGTACAG TGCAGAGAAT TTACGTCGCA TCTCACGCAG TCTCAGTGGC 
ACCGTTGTCT CAGAGAGGGA GGAAGCTCCG GTTTCTTCCC ACAGTTTTGA TTCATCAAAC 
GTGAGGAAGC CTTTGGAAAC CGGGCACCGT TGTTCCAGCT CCTCTTCCCT CCCTGTCATC 
CATGACCCTT CTGTGTTTCT CCTCGGTCCC CAACTCTACC TTCCCCAACC ACAGTTCCTG 
TCCCCAGATG TCCTGATGCC CACCATGGCA GGGGAGCCCA ATAGACTCCC AGGAACTTCA 
AGGAGTGTCC AGCAGTTTCT GGCTATGTGT GACAGGGGTG AAACTTCCCA AGGGGCCAAG 
TACACAGGAA GGACTTTGAA CTACCAGAGC CTCCCCCATC GCTCCAGAAC AGACAACTCC 
TGGGCACCCT GGTCAGAGAC CAACCAGCAT ATTGGGACCA GATTCCTGAC TACTCCAGGG 
TGCAATCCTC AACTAACCTA CACTGCCACA CTACCAGAAA GAAGCAAGGG CCTTCAGGTT 
CCTCACACTC AGTCCTGGAG TGATCTTTTC CATTCACCCT CCCACCCTCC CATTGTTCAT 
CCTGTGTACC CACCATCTAG CAGTCTTCAT GTACCCCTGA GGTCAGCTTG GAATTCAGAT 
CCTGTTCCAG GGTCCCGAAC CCCTGGTCCT CGAAGAGTAG ATATGCCCCC AGATGATGAC 
TGGAGGCAAA GCAGTTATGC CTCCCACTCT GGACACAGGA GAACAGTGGG AGAGGGGTTT 
CTGTTTGTTC TATCAGATGC TCCCAGAAGA GAGCAGATCA GGGCTAGAGT CCTGCAGCAC 
AGTCAATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015773.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871262.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.

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CloneID	OHu91147
Clone ID Related Accession (Same CDS sequence)	XM_017015773.1
Accession Version	XM_017015773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATGTGCG TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871262.1
CDS	3267..8327
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015773.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATGTGCG TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015778.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871267.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.

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CloneID	OHu91148
Clone ID Related Accession (Same CDS sequence)	XM_017015778.1
Accession Version	XM_017015778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871267.1
CDS	3267..8273
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015778.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGCTACACC TTGCCGAGGC 
CTCAGCAGGG AGTGTGGGGA GGATGAGCAG TACAGTGCAG AGAATTTACG TCGCATCTCA 
CGCAGTCTCA GTGGCACCGT TGTCTCAGAG AGGGAGGAAG CTCCGGTTTC TTCCCACAGT 
TTTGATTCAT CAAACGTGAG GAAGCCTTTG GAAACCGGGC ACCGTTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA CCCTTCTGTG TTTCTCCTCG GTCCCCAACT CTACCTTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCACCA TGGCAGGGGA GCCCAATAGA 
CTCCCAGGAA CTTCAAGGAG TGTCCAGCAG TTTCTGGCTA TGTGTGACAG GGGTGAAACT 
TCCCAAGGGG CCAAGTACAC AGGAAGGACT TTGAACTACC AGAGCCTCCC CCATCGCTCC 
AGAACAGACA ACTCCTGGGC ACCCTGGTCA GAGACCAACC AGCATATTGG GACCAGATTC 
CTGACTACTC CAGGGTGCAA TCCTCAACTA ACCTACACTG CCACACTACC AGAAAGAAGC 
AAGGGCCTTC AGGTTCCTCA CACTCAGTCC TGGAGTGATC TTTTCCATTC ACCCTCCCAC 
CCTCCCATTG TTCATCCTGT GTACCCACCA TCTAGCAGTC TTCATGTACC CCTGAGGTCA 
GCTTGGAATT CAGATCCTGT TCCAGGGTCC CGAACCCCTG GTCCTCGAAG AGTAGATATG 
CCCCCAGATG ATGACTGGAG GCAAAGCAGT TATGCCTCCC ACTCTGGACA CAGGAGAACA 
GTGGGAGAGG GGTTTCTGTT TGTTCTATCA GATGCTCCCA GAAGAGAGCA GATCAGGGCT 
AGAGTCCTGC AGCACAGTCA ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871268.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu91149
Clone ID Related Accession (Same CDS sequence)	XM_017015779.1
Accession Version	XM_017015779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871268.1
CDS	3267..8252
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015779.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015780.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871269.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.

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CloneID	OHu91150
Clone ID Related Accession (Same CDS sequence)	XM_017015780.1
Accession Version	XM_017015780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGAT TCATCAAACG TGAGGAAGCC TTTGGAAACC 
GGGCACCGTT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGACCCTTC TGTGTTTCTC 
CTCGGTCCCC AACTCTACCT TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
ACCATGGCAG GGGAGCCCAA TAGACTCCCA GGAACTTCAA GGAGTGTCCA GCAGTTTCTG 
GCTATGTGTG ACAGGGGTGA AACTTCCCAA GGGGCCAAGT ACACAGGAAG GACTTTGAAC 
TACCAGAGCC TCCCCCATCG CTCCAGAACA GACAACTCCT GGGCACCCTG GTCAGAGACC 
AACCAGCATA TTGGGACCAG ATTCCTGACT ACTCCAGGGT GCAATCCTCA ACTAACCTAC 
ACTGCCACAC TACCAGAAAG AAGCAAGGGC CTTCAGGTTC CTCACACTCA GTCCTGGAGT 
GATCTTTTCC ATTCACCCTC CCACCCTCCC ATTGTTCATC CTGTGTACCC ACCATCTAGC 
AGTCTTCATG TACCCCTGAG GTCAGCTTGG AATTCAGATC CTGTTCCAGG GTCCCGAACC 
CCTGGTCCTC GAAGAGTAGA TATGCCCCCA GATGATGACT GGAGGCAAAG CAGTTATGCC 
TCCCACTCTG GACACAGGAG AACAGTGGGA GAGGGGTTTC TGTTTGTTCT ATCAGATGCT 
CCCAGAAGAG AGCAGATCAG GGCTAGAGTC CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871269.1
CDS	3267..8237
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015780.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGGGAGGA GTGACGGCTT TGAGAGGTCC 
CTGCAAGAGG CAGAGTCAGT GTTTGAAGAG TCACTACATC TGGAACAGAA AGGAGACTGT 
GCTGCAGCTT TGGCTCTCTG TAATGAAGCT ATCTCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGAT TCATCAAACG TGAGGAAGCC TTTGGAAACC 
GGGCACCGTT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGACCCTTC TGTGTTTCTC 
CTCGGTCCCC AACTCTACCT TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
ACCATGGCAG GGGAGCCCAA TAGACTCCCA GGAACTTCAA GGAGTGTCCA GCAGTTTCTG 
GCTATGTGTG ACAGGGGTGA AACTTCCCAA GGGGCCAAGT ACACAGGAAG GACTTTGAAC 
TACCAGAGCC TCCCCCATCG CTCCAGAACA GACAACTCCT GGGCACCCTG GTCAGAGACC 
AACCAGCATA TTGGGACCAG ATTCCTGACT ACTCCAGGGT GCAATCCTCA ACTAACCTAC 
ACTGCCACAC TACCAGAAAG AAGCAAGGGC CTTCAGGTTC CTCACACTCA GTCCTGGAGT 
GATCTTTTCC ATTCACCCTC CCACCCTCCC ATTGTTCATC CTGTGTACCC ACCATCTAGC 
AGTCTTCATG TACCCCTGAG GTCAGCTTGG AATTCAGATC CTGTTCCAGG GTCCCGAACC 
CCTGGTCCTC GAAGAGTAGA TATGCCCCCA GATGATGACT GGAGGCAAAG CAGTTATGCC 
TCCCACTCTG GACACAGGAG AACAGTGGGA GAGGGGTTTC TGTTTGTTCT ATCAGATGCT 
CCCAGAAGAG AGCAGATCAG GGCTAGAGTC CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015781.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871270.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.

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CloneID	OHu91151
Clone ID Related Accession (Same CDS sequence)	XM_017015781.1
Accession Version	XM_017015781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGACA CCTGACTGAT AGTGAATGTA ATCAGAAACA CACATCCAAG 
AAAGGGTCAC TGATAGAGCG CAAGAGGAGC TCTGGTCGGG TTAGGAGGAA AGGCGATGAG 
CCCCAGGCCT CGGGATACCA CAGTGAAGGA GAAACACTGA AAGAGAAGCA GGCTCCTAGA 
AATGCCTCCA AACCATCCAG CAGCACCAAC AGGCTGAGAG ATTTTAAAGA GACAGTCAGC 
AATATGATCC ATAACAGACC ATCCCTGGCT TCTCAGACCA ATGTAGGCTC TCACTGCAGG 
GGCAGAGGAG GAGACCAGCC TGACAAAAAA CCTCCTAGGA CCCTGCCTTT ACACTCTCGT 
GACTGGGAAA TAGAGAGTAC CAGCAGTGAG TCAAAATCCA GTTCTTCCAG CAAGTATCGT 
CCCACATGGA GACCCAAACG AGAATCTCTG AATATTGACA GTATCTTTAG TAAGGACAAA 
AGGAAGCACT GTGGCTATAC CCAGCTTAGC CCCTTTTCTG AGGATTCAGC TAAAGAATTT 
ATACCAGATG AACCAAGCAA GCCACCTTCT TACGACATTA AATTTGGTGG ACCAAGCCCC 
CAGTACAAGC GCTGGGGCCC AGCACGGCCA GGCTCTCACC TTTTAGAGCA GCACCCCCGA 
CTAATCCAGC GAATGGAATC TGGCTATGAA AGCAGTGAGA GGAACAGCAG CAGCCCTGTC 
AGCCTGGATG CAGCCCTGCC TGAGAGCTCA AATGTCTACA GGGATCCAAG TGCTAAGAGA 
TCAGCTGGGT TGGTTCCTTC CTGGCGTCAT ATCCCAAAGT CGCACAGCAG TAGCATCCTG 
GAGGTAGACT CCACAGCATC CATGGGTGGC TGGACAAAGA GTCAGCCTTT CTCTGGTGAG 
GAGATATCTT CTAAAAGTGA ACTGGATGAA TTGCAGGAAG AGGTGGCCAG GAGGGCGCAG 
GAACAGGAAC TTCGAAGAAA ACGGGAGAAG GAGTTAGAGG CAGCGAAAGG GTTTAACCCT 
CATCCTAGCC GCTTCATGGA CTTGGATGAA CTGCAGAATC AGGGGAGGAG TGACGGCTTT 
GAGAGGTCCC TGCAAGAGGC AGAGTCAGTG TTTGAAGAGT CACTACATCT GGAACAGAAA 
GGAGACTGTG CTGCAGCTTT GGCTCTCTGT AATGAAGCTA TCTCTAAACT AAGACTTGCC 
CTGCATGGTG CCAGCTGTAG CACGCACAGC AGAGCCCTAG TCGATAAGAA GTTGCAAATC 
AGTATTCGAA AAGCACGGAG CCTGCAGGAT CGCATGCAGC AGCAGCAATC ACCACAGCAG 
CCGTCGCAGC CCTCAGCCTG CCTCCCAACA CAGGCGGGGA CTCTCTCTCA GCCAACAAGT 
GAACAGCCTA TCCCGCTCCA AGTATTGTTA AGCCAAGAGG CCCAACTGGA ATCCGGCATG 
GATACAGAGT TTGGGGCCAG TTCTTTCTTC CATTCACCTG CTTCCTGCCA TGAGTCACAC 
TCATCACTAT CTCCAGAGTC ATCTGCCCCA CAGCACAGCT CCCCCAGTAG ATCTGCCTTG 
AAGCTTCTGA CTTCGGTTGA AGTAGACAAC ATTGAACCCT CTGCATTCCA CAGGCAAGGT 
TTACCTAAAG CACCAGGGTG GACTGAGAAG AATTCTCATC ATAGTTGGGA GCCATTGGAT 
GCCCCAGAGG GTAAGCTGCA AGGCTCTAGG TGTGACAACA GCAGTTGCAG CAAGCTCCCT 
CCACAAGAAG GAAGAGGCAT TGCTCAAGAA CAGCTGTTCC AAGAAAAGAA GGATCCTGCT 
AACCCCTCCC CGGTGATGCC TGGAATAGCC ACCTCTGAGA GGGGTGATGA ACACAGCCTA 
GGCTGTAGTC CTTCAAATTC ATCAGCTCAG CCCAGCCTTC CCCTGTATAG AACCTGCCAC 
CCCATAATGC CTGTTGCTTC TTCATTTGTG CTTCACTGTC CTGATCCTGT GCAGAAAACT 
AACCAATGCC TCCAAGGCCA AAGCCTCAAA ACTTCATTGA CTTTAAAAGT GGACAGAGGC 
AGTGAGGAGA CCTATAGGCC AGAGTTTCCC AGCACAAAGG GGCTTGTCCG TTCTCTGGCT 
GAGCAGTTCC AGAGGATGCA GGGTGTCTCC ATGAGGGATA GTACAGGTTT CAAGGATAGA 
AGTTTGTCAG GTAGTCTAAG GAAGAACTCT TCCCCTTCTG ATTCTAAGCC TCCTTTCTCA 
CAGGGTCAAG AGAAAGGCCA CTGGCCATGG GCAAAGCAAC AATCCTCTCT GGAGGGTGGG 
GATAGACCAC TTTCCTGGGA AGAGTCCACT GAACATTCTT CTCTTGCCTT AAACTCTGGG 
CTGCCTAATG GTGAAACTTC TAGCGGAGGA CAGCCCAGGT TGGCAGAGCC AGACATATAC 
CAAGAGAAGC TGTCCCAAGT GAGAGATGTT AGGTCTAAGG ATCTGGGCAG CAGTACTGAC 
TTGGGGACTT CCTTGCCTTT GGATTCCTGG GTGAATATCA CAAGGTTCTG TGATTCTCAG 
CTTAAGCATG GGGCACCTAG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACGTGT 
GTAACCTATC CAGAGAGAAA TCACATCCTT TTGCATCCAC ATTGGAACCA AGACACAGAG 
CAGGAGACCT CAGAATTGGA GTCTCTGTAT CAGGCCAGTC TTCAGGCTTC TCAAGCTGGC 
TGTTCTGGAT GGGGGCAGCA GGATACCGCC TGGCACCCAC TTAGCCAAAC AGGCTCTGCA 
GATGGCATGG GGAGGAGGTT GCACTCAGCC CATGATCCTG GTCTCTCAAA GACTTCAACA 
GCAGAAATGG AGCATGGTCT CCATGAAGCC AGAACAGTGC GTACTTCTCA GGCTACACCT 
TGCCGAGGCC TCAGCAGGGA GTGTGGGGAG GATGAGCAGT ACAGTGCAGA GAATTTACGT 
CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAAGC TCCGGTTTCT 
TCCCACAGTT TTGATTCATC AAACGTGAGG AAGCCTTTGG AAACCGGGCA CCGTTGTTCC 
AGCTCCTCTT CCCTCCCTGT CATCCATGAC CCTTCTGTGT TTCTCCTCGG TCCCCAACTC 
TACCTTCCCC AACCACAGTT CCTGTCCCCA GATGTCCTGA TGCCCACCAT GGCAGGGGAG 
CCCAATAGAC TCCCAGGAAC TTCAAGGAGT GTCCAGCAGT TTCTGGCTAT GTGTGACAGG 
GGTGAAACTT CCCAAGGGGC CAAGTACACA GGAAGGACTT TGAACTACCA GAGCCTCCCC 
CATCGCTCCA GAACAGACAA CTCCTGGGCA CCCTGGTCAG AGACCAACCA GCATATTGGG 
ACCAGATTCC TGACTACTCC AGGGTGCAAT CCTCAACTAA CCTACACTGC CACACTACCA 
GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC ACTCAGTCCT GGAGTGATCT TTTCCATTCA 
CCCTCCCACC CTCCCATTGT TCATCCTGTG TACCCACCAT CTAGCAGTCT TCATGTACCC 
CTGAGGTCAG CTTGGAATTC AGATCCTGTT CCAGGGTCCC GAACCCCTGG TCCTCGAAGA 
GTAGATATGC CCCCAGATGA TGACTGGAGG CAAAGCAGTT ATGCCTCCCA CTCTGGACAC 
AGGAGAACAG TGGGAGAGGG GTTTCTGTTT GTTCTATCAG ATGCTCCCAG AAGAGAGCAG 
ATCAGGGCTA GAGTCCTGCA GCACAGTCAA TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871270.1
CDS	3267..8222
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015781.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGACA CCTGACTGAT AGTGAATGTA ATCAGAAACA CACATCCAAG 
AAAGGGTCAC TGATAGAGCG CAAGAGGAGC TCTGGTCGGG TTAGGAGGAA AGGCGATGAG 
CCCCAGGCCT CGGGATACCA CAGTGAAGGA GAAACACTGA AAGAGAAGCA GGCTCCTAGA 
AATGCCTCCA AACCATCCAG CAGCACCAAC AGGCTGAGAG ATTTTAAAGA GACAGTCAGC 
AATATGATCC ATAACAGACC ATCCCTGGCT TCTCAGACCA ATGTAGGCTC TCACTGCAGG 
GGCAGAGGAG GAGACCAGCC TGACAAAAAA CCTCCTAGGA CCCTGCCTTT ACACTCTCGT 
GACTGGGAAA TAGAGAGTAC CAGCAGTGAG TCAAAATCCA GTTCTTCCAG CAAGTATCGT 
CCCACATGGA GACCCAAACG AGAATCTCTG AATATTGACA GTATCTTTAG TAAGGACAAA 
AGGAAGCACT GTGGCTATAC CCAGCTTAGC CCCTTTTCTG AGGATTCAGC TAAAGAATTT 
ATACCAGATG AACCAAGCAA GCCACCTTCT TACGACATTA AATTTGGTGG ACCAAGCCCC 
CAGTACAAGC GCTGGGGCCC AGCACGGCCA GGCTCTCACC TTTTAGAGCA GCACCCCCGA 
CTAATCCAGC GAATGGAATC TGGCTATGAA AGCAGTGAGA GGAACAGCAG CAGCCCTGTC 
AGCCTGGATG CAGCCCTGCC TGAGAGCTCA AATGTCTACA GGGATCCAAG TGCTAAGAGA 
TCAGCTGGGT TGGTTCCTTC CTGGCGTCAT ATCCCAAAGT CGCACAGCAG TAGCATCCTG 
GAGGTAGACT CCACAGCATC CATGGGTGGC TGGACAAAGA GTCAGCCTTT CTCTGGTGAG 
GAGATATCTT CTAAAAGTGA ACTGGATGAA TTGCAGGAAG AGGTGGCCAG GAGGGCGCAG 
GAACAGGAAC TTCGAAGAAA ACGGGAGAAG GAGTTAGAGG CAGCGAAAGG GTTTAACCCT 
CATCCTAGCC GCTTCATGGA CTTGGATGAA CTGCAGAATC AGGGGAGGAG TGACGGCTTT 
GAGAGGTCCC TGCAAGAGGC AGAGTCAGTG TTTGAAGAGT CACTACATCT GGAACAGAAA 
GGAGACTGTG CTGCAGCTTT GGCTCTCTGT AATGAAGCTA TCTCTAAACT AAGACTTGCC 
CTGCATGGTG CCAGCTGTAG CACGCACAGC AGAGCCCTAG TCGATAAGAA GTTGCAAATC 
AGTATTCGAA AAGCACGGAG CCTGCAGGAT CGCATGCAGC AGCAGCAATC ACCACAGCAG 
CCGTCGCAGC CCTCAGCCTG CCTCCCAACA CAGGCGGGGA CTCTCTCTCA GCCAACAAGT 
GAACAGCCTA TCCCGCTCCA AGTATTGTTA AGCCAAGAGG CCCAACTGGA ATCCGGCATG 
GATACAGAGT TTGGGGCCAG TTCTTTCTTC CATTCACCTG CTTCCTGCCA TGAGTCACAC 
TCATCACTAT CTCCAGAGTC ATCTGCCCCA CAGCACAGCT CCCCCAGTAG ATCTGCCTTG 
AAGCTTCTGA CTTCGGTTGA AGTAGACAAC ATTGAACCCT CTGCATTCCA CAGGCAAGGT 
TTACCTAAAG CACCAGGGTG GACTGAGAAG AATTCTCATC ATAGTTGGGA GCCATTGGAT 
GCCCCAGAGG GTAAGCTGCA AGGCTCTAGG TGTGACAACA GCAGTTGCAG CAAGCTCCCT 
CCACAAGAAG GAAGAGGCAT TGCTCAAGAA CAGCTGTTCC AAGAAAAGAA GGATCCTGCT 
AACCCCTCCC CGGTGATGCC TGGAATAGCC ACCTCTGAGA GGGGTGATGA ACACAGCCTA 
GGCTGTAGTC CTTCAAATTC ATCAGCTCAG CCCAGCCTTC CCCTGTATAG AACCTGCCAC 
CCCATAATGC CTGTTGCTTC TTCATTTGTG CTTCACTGTC CTGATCCTGT GCAGAAAACT 
AACCAATGCC TCCAAGGCCA AAGCCTCAAA ACTTCATTGA CTTTAAAAGT GGACAGAGGC 
AGTGAGGAGA CCTATAGGCC AGAGTTTCCC AGCACAAAGG GGCTTGTCCG TTCTCTGGCT 
GAGCAGTTCC AGAGGATGCA GGGTGTCTCC ATGAGGGATA GTACAGGTTT CAAGGATAGA 
AGTTTGTCAG GTAGTCTAAG GAAGAACTCT TCCCCTTCTG ATTCTAAGCC TCCTTTCTCA 
CAGGGTCAAG AGAAAGGCCA CTGGCCATGG GCAAAGCAAC AATCCTCTCT GGAGGGTGGG 
GATAGACCAC TTTCCTGGGA AGAGTCCACT GAACATTCTT CTCTTGCCTT AAACTCTGGG 
CTGCCTAATG GTGAAACTTC TAGCGGAGGA CAGCCCAGGT TGGCAGAGCC AGACATATAC 
CAAGAGAAGC TGTCCCAAGT GAGAGATGTT AGGTCTAAGG ATCTGGGCAG CAGTACTGAC 
TTGGGGACTT CCTTGCCTTT GGATTCCTGG GTGAATATCA CAAGGTTCTG TGATTCTCAG 
CTTAAGCATG GGGCACCTAG GCCAGGAATG AAGTCCTCCC CTCATGATTC CCATACGTGT 
GTAACCTATC CAGAGAGAAA TCACATCCTT TTGCATCCAC ATTGGAACCA AGACACAGAG 
CAGGAGACCT CAGAATTGGA GTCTCTGTAT CAGGCCAGTC TTCAGGCTTC TCAAGCTGGC 
TGTTCTGGAT GGGGGCAGCA GGATACCGCC TGGCACCCAC TTAGCCAAAC AGGCTCTGCA 
GATGGCATGG GGAGGAGGTT GCACTCAGCC CATGATCCTG GTCTCTCAAA GACTTCAACA 
GCAGAAATGG AGCATGGTCT CCATGAAGCC AGAACAGTGC GTACTTCTCA GGCTACACCT 
TGCCGAGGCC TCAGCAGGGA GTGTGGGGAG GATGAGCAGT ACAGTGCAGA GAATTTACGT 
CGCATCTCAC GCAGTCTCAG TGGCACCGTT GTCTCAGAGA GGGAGGAAGC TCCGGTTTCT 
TCCCACAGTT TTGATTCATC AAACGTGAGG AAGCCTTTGG AAACCGGGCA CCGTTGTTCC 
AGCTCCTCTT CCCTCCCTGT CATCCATGAC CCTTCTGTGT TTCTCCTCGG TCCCCAACTC 
TACCTTCCCC AACCACAGTT CCTGTCCCCA GATGTCCTGA TGCCCACCAT GGCAGGGGAG 
CCCAATAGAC TCCCAGGAAC TTCAAGGAGT GTCCAGCAGT TTCTGGCTAT GTGTGACAGG 
GGTGAAACTT CCCAAGGGGC CAAGTACACA GGAAGGACTT TGAACTACCA GAGCCTCCCC 
CATCGCTCCA GAACAGACAA CTCCTGGGCA CCCTGGTCAG AGACCAACCA GCATATTGGG 
ACCAGATTCC TGACTACTCC AGGGTGCAAT CCTCAACTAA CCTACACTGC CACACTACCA 
GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC ACTCAGTCCT GGAGTGATCT TTTCCATTCA 
CCCTCCCACC CTCCCATTGT TCATCCTGTG TACCCACCAT CTAGCAGTCT TCATGTACCC 
CTGAGGTCAG CTTGGAATTC AGATCCTGTT CCAGGGTCCC GAACCCCTGG TCCTCGAAGA 
GTAGATATGC CCCCAGATGA TGACTGGAGG CAAAGCAGTT ATGCCTCCCA CTCTGGACAC 
AGGAGAACAG TGGGAGAGGG GTTTCTGTTT GTTCTATCAG ATGCTCCCAG AAGAGAGCAG 
ATCAGGGCTA GAGTCCTGCA GCACAGTCAA TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015782.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871271.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.

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CloneID	OHu91152
Clone ID Related Accession (Same CDS sequence)	XM_017015782.1 , XM_017015782.2
Accession Version	XM_017015782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871271.1
CDS	1039..5973
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015782.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015782.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871271.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X24, mRNA.

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CloneID	OHu91152
Clone ID Related Accession (Same CDS sequence)	XM_017015782.1 , XM_017015782.2
Accession Version	XM_017015782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871271.1
CDS	1552..6486
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015782.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGGAGGG AGCCCTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTCCCACCA TGAGTCTGTG GTCAGTCACT TCTCTTCTGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA AAATGATTCC ATGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCCAAGA AAGGGTCACT GATAGAGCGC 
AAGAGGAGCT CTGGTCGGGT TAGGAGGAAA GGCGATGAGC CCCAGGCCTC GGGATACCAC 
AGTGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGAA ATGCCTCCAA ACCATCCAGC 
AGCACCAACA GGCTGAGAGA TTTTAAAGAG ACAGTCAGCA ATATGATCCA TAACAGACCA 
TCCCTGGCTT CTCAGACCAA TGTAGGCTCT CACTGCAGGG GCAGAGGAGG AGACCAGCCT 
GACAAAAAAC CTCCTAGGAC CCTGCCTTTA CACTCTCGTG ACTGGGAAAT AGAGAGTACC 
AGCAGTGAGT CAAAATCCAG TTCTTCCAGC AAGTATCGTC CCACATGGAG ACCCAAACGA 
GAATCTCTGA ATATTGACAG TATCTTTAGT AAGGACAAAA GGAAGCACTG TGGCTATACC 
CAGCTTAGCC CCTTTTCTGA GGATTCAGCT AAAGAATTTA TACCAGATGA ACCAAGCAAG 
CCACCTTCTT ACGACATTAA ATTTGGTGGA CCAAGCCCCC AGTACAAGCG CTGGGGCCCA 
GCACGGCCAG GCTCTCACCT TTTAGAGCAG CACCCCCGAC TAATCCAGCG AATGGAATCT 
GGCTATGAAA GCAGTGAGAG GAACAGCAGC AGCCCTGTCA GCCTGGATGC AGCCCTGCCT 
GAGAGCTCAA ATGTCTACAG GGATCCAAGT GCTAAGAGAT CAGCTGGGTT GGTTCCTTCC 
TGGCGTCATA TCCCAAAGTC GCACAGCAGT AGCATCCTGG AGGTAGACTC CACAGCATCC 
ATGGGTGGCT GGACAAAGAG TCAGCCTTTC TCTGGTGAGG AGATATCTTC TAAAAGTGAA 
CTGGATGAAT TGCAGGAAGA GGTGGCCAGG AGGGCGCAGG AACAGGAACT TCGAAGAAAA 
CGGGAGAAGG AGTTAGAGGC AGCGAAAGGG TTTAACCCTC ATCCTAGCCG CTTCATGGAC 
TTGGATGAAC TGCAGAATCA GGCTAAACTA AGACTTGCCC TGCATGGTGC CAGCTGTAGC 
ACGCACAGCA GAGCCCTAGT CGATAAGAAG TTGCAAATCA GTATTCGAAA AGCACGGAGC 
CTGCAGGATC GCATGCAGCA GCAGCAATCA CCACAGCAGC CGTCGCAGCC CTCAGCCTGC 
CTCCCAACAC AGGCGGGGAC TCTCTCTCAG CCAACAAGTG AACAGCCTAT CCCGCTCCAA 
GTATTGTTAA GCCAAGAGGC CCAACTGGAA TCCGGCATGG ATACAGAGTT TGGGGCCAGT 
TCTTTCTTCC ATTCACCTGC TTCCTGCCAT GAGTCACACT CATCACTATC TCCAGAGTCA 
TCTGCCCCAC AGCACAGCTC CCCCAGTAGA TCTGCCTTGA AGCTTCTGAC TTCGGTTGAA 
GTAGACAACA TTGAACCCTC TGCATTCCAC AGGCAAGGTT TACCTAAAGC ACCAGGGTGG 
ACTGAGAAGA ATTCTCATCA TAGTTGGGAG CCATTGGATG CCCCAGAGGG TAAGCTGCAA 
GGCTCTAGGT GTGACAACAG CAGTTGCAGC AAGCTCCCTC CACAAGAAGG AAGAGGCATT 
GCTCAAGAAC AGCTGTTCCA AGAAAAGAAG GATCCTGCTA ACCCCTCCCC GGTGATGCCT 
GGAATAGCCA CCTCTGAGAG GGGTGATGAA CACAGCCTAG GCTGTAGTCC TTCAAATTCA 
TCAGCTCAGC CCAGCCTTCC CCTGTATAGA ACCTGCCACC CCATAATGCC TGTTGCTTCT 
TCATTTGTGC TTCACTGTCC TGATCCTGTG CAGAAAACTA ACCAATGCCT CCAAGGCCAA 
AGCCTCAAAA CTTCATTGAC TTTAAAAGTG GACAGAGGCA GTGAGGAGAC CTATAGGCCA 
GAGTTTCCCA GCACAAAGGG GCTTGTCCGT TCTCTGGCTG AGCAGTTCCA GAGGATGCAG 
GGTGTCTCCA TGAGGGATAG TACAGGTTTC AAGGATAGAA GTTTGTCAGG TAGTCTAAGG 
AAGAACTCTT CCCCTTCTGA TTCTAAGCCT CCTTTCTCAC AGGGTCAAGA GAAAGGCCAC 
TGGCCATGGG CAAAGCAACA ATCCTCTCTG GAGGGTGGGG ATAGACCACT TTCCTGGGAA 
GAGTCCACTG AACATTCTTC TCTTGCCTTA AACTCTGGGC TGCCTAATGG TGAAACTTCT 
AGCGGAGGAC AGCCCAGGTT GGCAGAGCCA GACATATACC AAGAGAAGCT GTCCCAAGTG 
AGAGATGTTA GGTCTAAGGA TCTGGGCAGC AGTACTGACT TGGGGACTTC CTTGCCTTTG 
GATTCCTGGG TGAATATCAC AAGGTTCTGT GATTCTCAGC TTAAGCATGG GGCACCTAGG 
CCAGGAATGA AGTCCTCCCC TCATGATTCC CATACGTGTG TAACCTATCC AGAGAGAAAT 
CACATCCTTT TGCATCCACA TTGGAACCAA GACACAGAGC AGGAGACCTC AGAATTGGAG 
TCTCTGTATC AGGCCAGTCT TCAGGCTTCT CAAGCTGGCT GTTCTGGATG GGGGCAGCAG 
GATACCGCCT GGCACCCACT TAGCCAAACA GGCTCTGCAG ATGGCATGGG GAGGAGGTTG 
CACTCAGCCC ATGATCCTGG TCTCTCAAAG ACTTCAACAG CAGAAATGGA GCATGGTCTC 
CATGAAGCCA GAACAGTGCG TACTTCTCAG GCTACACCTT GCCGAGGCCT CAGCAGGGAG 
TGTGGGGAGG ATGAGCAGTA CAGTGCAGAG AATTTACGTC GCATCTCACG CAGTCTCAGT 
GGCACCGTTG TCTCAGAGAG GGAGGAAGCT CCGGTTTCTT CCCACAGTTT TGATTCATCA 
AACGTGAGGA AGCCTTTGGA AACCGGGCAC CGTTGTTCCA GCTCCTCTTC CCTCCCTGTC 
ATCCATGACC CTTCTGTGTT TCTCCTCGGT CCCCAACTCT ACCTTCCCCA ACCACAGTTC 
CTGTCCCCAG ATGTCCTGAT GCCCACCATG GCAGGGGAGC CCAATAGACT CCCAGGAACT 
TCAAGGAGTG TCCAGCAGTT TCTGGCTATG TGTGACAGGG GTGAAACTTC CCAAGGGGCC 
AAGTACACAG GAAGGACTTT GAACTACCAG AGCCTCCCCC ATCGCTCCAG AACAGACAAC 
TCCTGGGCAC CCTGGTCAGA GACCAACCAG CATATTGGGA CCAGATTCCT GACTACTCCA 
GGGTGCAATC CTCAACTAAC CTACACTGCC ACACTACCAG AAAGAAGCAA GGGCCTTCAG 
GTTCCTCACA CTCAGTCCTG GAGTGATCTT TTCCATTCAC CCTCCCACCC TCCCATTGTT 
CATCCTGTGT ACCCACCATC TAGCAGTCTT CATGTACCCC TGAGGTCAGC TTGGAATTCA 
GATCCTGTTC CAGGGTCCCG AACCCCTGGT CCTCGAAGAG TAGATATGCC CCCAGATGAT 
GACTGGAGGC AAAGCAGTTA TGCCTCCCAC TCTGGACACA GGAGAACAGT GGGAGAGGGG 
TTTCTGTTTG TTCTATCAGA TGCTCCCAGA AGAGAGCAGA TCAGGGCTAG AGTCCTGCAG 
CACAGTCAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015783.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871272.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.

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CloneID	OHu91153
Clone ID Related Accession (Same CDS sequence)	XM_017015783.1 , XM_017015784.1
Accession Version	XM_017015783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871272.1
CDS	2137..7020
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015783.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015784.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871273.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu91153
Clone ID Related Accession (Same CDS sequence)	XM_017015783.1 , XM_017015784.1
Accession Version	XM_017015784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871273.1
CDS	83..4966
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015784.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG CTACACCTTG CCGAGGCCTC 
AGCAGGGAGT GTGGGGAGGA TGAGCAGTAC AGTGCAGAGA ATTTACGTCG CATCTCACGC 
AGTCTCAGTG GCACCGTTGT CTCAGAGAGG GAGGAAGCTC CGGTTTCTTC CCACAGTTTT 
GATTCATCAA ACGTGAGGAA GCCTTTGGAA ACCGGGCACC GTTGTTCCAG CTCCTCTTCC 
CTCCCTGTCA TCCATGACCC TTCTGTGTTT CTCCTCGGTC CCCAACTCTA CCTTCCCCAA 
CCACAGTTCC TGTCCCCAGA TGTCCTGATG CCCACCATGG CAGGGGAGCC CAATAGACTC 
CCAGGAACTT CAAGGAGTGT CCAGCAGTTT CTGGCTATGT GTGACAGGGG TGAAACTTCC 
CAAGGGGCCA AGTACACAGG AAGGACTTTG AACTACCAGA GCCTCCCCCA TCGCTCCAGA 
ACAGACAACT CCTGGGCACC CTGGTCAGAG ACCAACCAGC ATATTGGGAC CAGATTCCTG 
ACTACTCCAG GGTGCAATCC TCAACTAACC TACACTGCCA CACTACCAGA AAGAAGCAAG 
GGCCTTCAGG TTCCTCACAC TCAGTCCTGG AGTGATCTTT TCCATTCACC CTCCCACCCT 
CCCATTGTTC ATCCTGTGTA CCCACCATCT AGCAGTCTTC ATGTACCCCT GAGGTCAGCT 
TGGAATTCAG ATCCTGTTCC AGGGTCCCGA ACCCCTGGTC CTCGAAGAGT AGATATGCCC 
CCAGATGATG ACTGGAGGCA AAGCAGTTAT GCCTCCCACT CTGGACACAG GAGAACAGTG 
GGAGAGGGGT TTCTGTTTGT TCTATCAGAT GCTCCCAGAA GAGAGCAGAT CAGGGCTAGA 
GTCCTGCAGC ACAGTCAATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015785.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871274.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.

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CloneID	OHu91154
Clone ID Related Accession (Same CDS sequence)	XM_017015785.1
Accession Version	XM_017015785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871274.1
CDS	3267..8132
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDVRLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQAKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015785.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TGTGCGTCTG 
TTTTTCAGAG TGACGGATGA CCGGGCCAAG CAATCTGAAC TGTACTTAGT TGGAATGATC 
TGTTACTATG GCAAACATTA TTCTACATTC TTTTTTCAAA CAAAGATTCG CAAATGGATG 
TATTTTGATG ATGCTCATGT CAAGGAGATT GGGCCCAAAT GGAAGGATGT GGTGACCAAA 
TGCATCAAGG GGCATTATCA GCCCCTGCTG CTGCTTTATG CAGATCCCCA GGGTACCCCA 
GTTTCCACCC AGGACCTGCC TCCCCAAGCT GAGTTCCAGT CATACAGCAG GACATGCTAC 
GACAGTGAAG ATTCAGGGAG GGAGCCCTCC ATCTCAAGTG ACACTCGAAC AGATTCCTCA 
ACGGAGAGCT ATCCCTACAA ACATTCCCAC CATGAGTCTG TGGTCAGTCA CTTCTCTTCT 
GATTCTCAGG GGACAGTCAT CTATAATGTG GAAAATGATT CCATGTCTCA GAGCAGTCGG 
GACACAGGAC ACCTGACTGA TAGTGAATGT AATCAGAAAC ACACATCCAA GAAAGGGTCA 
CTGATAGAGC GCAAGAGGAG CTCTGGTCGG GTTAGGAGGA AAGGCGATGA GCCCCAGGCC 
TCGGGATACC ACAGTGAAGG AGAAACACTG AAAGAGAAGC AGGCTCCTAG AAATGCCTCC 
AAACCATCCA GCAGCACCAA CAGGCTGAGA GATTTTAAAG AGACAGTCAG CAATATGATC 
CATAACAGAC CATCCCTGGC TTCTCAGACC AATGTAGGCT CTCACTGCAG GGGCAGAGGA 
GGAGACCAGC CTGACAAAAA ACCTCCTAGG ACCCTGCCTT TACACTCTCG TGACTGGGAA 
ATAGAGAGTA CCAGCAGTGA GTCAAAATCC AGTTCTTCCA GCAAGTATCG TCCCACATGG 
AGACCCAAAC GAGAATCTCT GAATATTGAC AGTATCTTTA GTAAGGACAA AAGGAAGCAC 
TGTGGCTATA CCCAGCTTAG CCCCTTTTCT GAGGATTCAG CTAAAGAATT TATACCAGAT 
GAACCAAGCA AGCCACCTTC TTACGACATT AAATTTGGTG GACCAAGCCC CCAGTACAAG 
CGCTGGGGCC CAGCACGGCC AGGCTCTCAC CTTTTAGAGC AGCACCCCCG ACTAATCCAG 
CGAATGGAAT CTGGCTATGA AAGCAGTGAG AGGAACAGCA GCAGCCCTGT CAGCCTGGAT 
GCAGCCCTGC CTGAGAGCTC AAATGTCTAC AGGGATCCAA GTGCTAAGAG ATCAGCTGGG 
TTGGTTCCTT CCTGGCGTCA TATCCCAAAG TCGCACAGCA GTAGCATCCT GGAGGTAGAC 
TCCACAGCAT CCATGGGTGG CTGGACAAAG AGTCAGCCTT TCTCTGGTGA GGAGATATCT 
TCTAAAAGTG AACTGGATGA ATTGCAGGAA GAGGTGGCCA GGAGGGCGCA GGAACAGGAA 
CTTCGAAGAA AACGGGAGAA GGAGTTAGAG GCAGCGAAAG GGTTTAACCC TCATCCTAGC 
CGCTTCATGG ACTTGGATGA ACTGCAGAAT CAGGCTAAAC TAAGACTTGC CCTGCATGGT 
GCCAGCTGTA GCACGCACAG CAGAGCCCTA GTCGATAAGA AGTTGCAAAT CAGTATTCGA 
AAAGCACGGA GCCTGCAGGA TCGCATGCAG CAGCAGCAAT CACCACAGCA GCCGTCGCAG 
CCCTCAGCCT GCCTCCCAAC ACAGGCGGGG ACTCTCTCTC AGCCAACAAG TGAACAGCCT 
ATCCCGCTCC AAGTATTGTT AAGCCAAGAG GCCCAACTGG AATCCGGCAT GGATACAGAG 
TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC ATGAGTCACA CTCATCACTA 
TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA GATCTGCCTT GAAGCTTCTG 
ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC ACAGGCAAGG TTTACCTAAA 
GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG AGCCATTGGA TGCCCCAGAG 
GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA GCAAGCTCCC TCCACAAGAA 
GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA AGGATCCTGC TAACCCCTCC 
CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG AACACAGCCT AGGCTGTAGT 
CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA GAACCTGCCA CCCCATAATG 
CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG TGCAGAAAAC TAACCAATGC 
CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG TGGACAGAGG CAGTGAGGAG 
ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC GTTCTCTGGC TGAGCAGTTC 
CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT TCAAGGATAG AAGTTTGTCA 
GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC CTCCTTTCTC ACAGGGTCAA 
GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC TGGAGGGTGG GGATAGACCA 
CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT TAAACTCTGG GCTGCCTAAT 
GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC CAGACATATA CCAAGAGAAG 
CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA GCAGTACTGA CTTGGGGACT 
TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT GTGATTCTCA GCTTAAGCAT 
GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT CCCATACGTG TGTAACCTAT 
CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC AAGACACAGA GCAGGAGACC 
TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT CTCAAGCTGG CTGTTCTGGA 
TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA CAGGCTCTGC AGATGGCATG 
GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA AGACTTCAAC AGCAGAAATG 
GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC AGGATTCATC AAACGTGAGG 
AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT CCCTCCCTGT CATCCATGAC 
CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC AACCACAGTT CCTGTCCCCA 
GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC TCCCAGGAAC TTCAAGGAGT 
GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT CCCAAGGGGC CAAGTACACA 
GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA GAACAGACAA CTCCTGGGCA 
CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC TGACTACTCC AGGGTGCAAT 
CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA AGGGCCTTCA GGTTCCTCAC 
ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC CTCCCATTGT TCATCCTGTG 
TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG CTTGGAATTC AGATCCTGTT 
CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC CCCCAGATGA TGACTGGAGG 
CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG TGGGAGAGGG GTTTCTGTTT 
GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA GAGTCCTGCA GCACAGTCAA 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447842.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303610.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303610.1
CDS	988..6108
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447842.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447842.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447835.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303603.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303603.1
CDS	1674..6794
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447835.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447835.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447836.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303604.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303604.1
CDS	2640..7760
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447836.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447837.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303605.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303605.1
CDS	1428..6548
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447837.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447837.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447838.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303606.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303606.1
CDS	723..5843
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447838.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447838.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447841.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303609.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303609.1
CDS	2302..7422
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447841.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447841.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447833.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303601.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303601.1
CDS	1934..7054
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447833.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447833.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447845.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303613.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303613.1
CDS	102..5222
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447845.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447832.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303600.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303600.1
CDS	2519..7639
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447832.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447832.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447834.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303602.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303602.1
CDS	2259..7379
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447834.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447834.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447843.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303611.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303611.1
CDS	83..5203
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447843.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447843.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447844.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303612.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303612.1
CDS	742..5862
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447844.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447844.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_017015771.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871260.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_017015771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871260.1
CDS	3267..8387
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_017015771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015771.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447839.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303607.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303607.1
CDS	784..5904
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447839.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447839.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_024447840.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303608.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

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CloneID	OHu44060
Clone ID Related Accession (Same CDS sequence)	XM_024447842.1 , XM_024447835.1 , XM_024447836.1 , XM_024447837.1 , XM_024447838.1 , XM_024447841.1 , XM_024447833.1 , XM_024447845.1 , XM_024447832.1 , XM_024447834.1 , XM_024447843.1 , XM_024447844.1 , XM_017015771.1 , XM_024447839.1 , XM_024447840.1
Accession Version	XM_024447840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303608.1
CDS	1552..6672
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_024447840.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447840.1

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GGAGGGAGCC CTCCATCTCA AGTGACACTC GAACAGATTC CTCAACGGAG 
AGCTATCCCT ACAAACATTC CCACCATGAG TCTGTGGTCA GTCACTTCTC TTCTGATTCT 
CAGGGGACAG TCATCTATAA TGTGGAAAAT GATTCCATGT CTCAGAGCAG TCGGGACACA 
GGACACCTGA CTGATAGTGA ATGTAATCAG AAACACACAT CCAAGAAAGG GTCACTGATA 
GAGCGCAAGA GGAGCTCTGG TCGGGTTAGG AGGAAAGGCG ATGAGCCCCA GGCCTCGGGA 
TACCACAGTG AAGGAGAAAC ACTGAAAGAG AAGCAGGCTC CTAGAAATGC CTCCAAACCA 
TCCAGCAGCA CCAACAGGCT GAGAGATTTT AAAGAGACAG TCAGCAATAT GATCCATAAC 
AGACCATCCC TGGCTTCTCA GACCAATGTA GGCTCTCACT GCAGGGGCAG AGGAGGAGAC 
CAGCCTGACA AAAAACCTCC TAGGACCCTG CCTTTACACT CTCGTGACTG GGAAATAGAG 
AGTACCAGCA GTGAGTCAAA ATCCAGTTCT TCCAGCAAGT ATCGTCCCAC ATGGAGACCC 
AAACGAGAAT CTCTGAATAT TGACAGTATC TTTAGTAAGG ACAAAAGGAA GCACTGTGGC 
TATACCCAGC TTAGCCCCTT TTCTGAGGAT TCAGCTAAAG AATTTATACC AGATGAACCA 
AGCAAGCCAC CTTCTTACGA CATTAAATTT GGTGGACCAA GCCCCCAGTA CAAGCGCTGG 
GGCCCAGCAC GGCCAGGCTC TCACCTTTTA GAGCAGCACC CCCGACTAAT CCAGCGAATG 
GAATCTGGCT ATGAAAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGCCT GGATGCAGCC 
CTGCCTGAGA GCTCAAATGT CTACAGGGAT CCAAGTGCTA AGAGATCAGC TGGGTTGGTT 
CCTTCCTGGC GTCATATCCC AAAGTCGCAC AGCAGTAGCA TCCTGGAGGT AGACTCCACA 
GCATCCATGG GTGGCTGGAC AAAGAGTCAG CCTTTCTCTG GTGAGGAGAT ATCTTCTAAA 
AGTGAACTGG ATGAATTGCA GGAAGAGGTG GCCAGGAGGG CGCAGGAACA GGAACTTCGA 
AGAAAACGGG AGAAGGAGTT AGAGGCAGCG AAAGGGTTTA ACCCTCATCC TAGCCGCTTC 
ATGGACTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAGAG GTCCCTGCAA 
GAGGCAGAGT CAGTGTTTGA AGAGTCACTA CATCTGGAAC AGAAAGGAGA CTGTGCTGCA 
GCTTTGGCTC TCTGTAATGA AGCTATCTCT AAACTAAGAC TTGCCCTGCA TGGTGCCAGC 
TGTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGGAGCCTGC AGGATCGCAT GCAGCAGCAG CAATCACCAC AGCAGCCGTC GCAGCCCTCA 
GCCTGCCTCC CAACACAGGC GGGGACTCTC TCTCAGCCAA CAAGTGAACA GCCTATCCCG 
CTCCAAGTAT TGTTAAGCCA AGAGGCCCAA CTGGAATCCG GCATGGATAC AGAGTTTGGG 
GCCAGTTCTT TCTTCCATTC ACCTGCTTCC TGCCATGAGT CACACTCATC ACTATCTCCA 
GAGTCATCTG CCCCACAGCA CAGCTCCCCC AGTAGATCTG CCTTGAAGCT TCTGACTTCG 
GTTGAAGTAG ACAACATTGA ACCCTCTGCA TTCCACAGGC AAGGTTTACC TAAAGCACCA 
GGGTGGACTG AGAAGAATTC TCATCATAGT TGGGAGCCAT TGGATGCCCC AGAGGGTAAG 
CTGCAAGGCT CTAGGTGTGA CAACAGCAGT TGCAGCAAGC TCCCTCCACA AGAAGGAAGA 
GGCATTGCTC AAGAACAGCT GTTCCAAGAA AAGAAGGATC CTGCTAACCC CTCCCCGGTG 
ATGCCTGGAA TAGCCACCTC TGAGAGGGGT GATGAACACA GCCTAGGCTG TAGTCCTTCA 
AATTCATCAG CTCAGCCCAG CCTTCCCCTG TATAGAACCT GCCACCCCAT AATGCCTGTT 
GCTTCTTCAT TTGTGCTTCA CTGTCCTGAT CCTGTGCAGA AAACTAACCA ATGCCTCCAA 
GGCCAAAGCC TCAAAACTTC ATTGACTTTA AAAGTGGACA GAGGCAGTGA GGAGACCTAT 
AGGCCAGAGT TTCCCAGCAC AAAGGGGCTT GTCCGTTCTC TGGCTGAGCA GTTCCAGAGG 
ATGCAGGGTG TCTCCATGAG GGATAGTACA GGTTTCAAGG ATAGAAGTTT GTCAGGTAGT 
CTAAGGAAGA ACTCTTCCCC TTCTGATTCT AAGCCTCCTT TCTCACAGGG TCAAGAGAAA 
GGCCACTGGC CATGGGCAAA GCAACAATCC TCTCTGGAGG GTGGGGATAG ACCACTTTCC 
TGGGAAGAGT CCACTGAACA TTCTTCTCTT GCCTTAAACT CTGGGCTGCC TAATGGTGAA 
ACTTCTAGCG GAGGACAGCC CAGGTTGGCA GAGCCAGACA TATACCAAGA GAAGCTGTCC 
CAAGTGAGAG ATGTTAGGTC TAAGGATCTG GGCAGCAGTA CTGACTTGGG GACTTCCTTG 
CCTTTGGATT CCTGGGTGAA TATCACAAGG TTCTGTGATT CTCAGCTTAA GCATGGGGCA 
CCTAGGCCAG GAATGAAGTC CTCCCCTCAT GATTCCCATA CGTGTGTAAC CTATCCAGAG 
AGAAATCACA TCCTTTTGCA TCCACATTGG AACCAAGACA CAGAGCAGGA GACCTCAGAA 
TTGGAGTCTC TGTATCAGGC CAGTCTTCAG GCTTCTCAAG CTGGCTGTTC TGGATGGGGG 
CAGCAGGATA CCGCCTGGCA CCCACTTAGC CAAACAGGCT CTGCAGATGG CATGGGGAGG 
AGGTTGCACT CAGCCCATGA TCCTGGTCTC TCAAAGACTT CAACAGCAGA AATGGAGCAT 
GGTCTCCATG AAGCCAGAAC AGTGCGTACT TCTCAGGCTA CACCTTGCCG AGGCCTCAGC 
AGGGAGTGTG GGGAGGATGA GCAGTACAGT GCAGAGAATT TACGTCGCAT CTCACGCAGT 
CTCAGTGGCA CCGTTGTCTC AGAGAGGGAG GAAGCTCCGG TTTCTTCCCA CAGTTTTGAT 
TCATCAAACG TGAGGAAGCC TTTGGAAACC GGGCACCGTT GTTCCAGCTC CTCTTCCCTC 
CCTGTCATCC ATGACCCTTC TGTGTTTCTC CTCGGTCCCC AACTCTACCT TCCCCAACCA 
CAGTTCCTGT CCCCAGATGT CCTGATGCCC ACCATGGCAG GGGAGCCCAA TAGACTCCCA 
GGAACTTCAA GGAGTGTCCA GCAGTTTCTG GCTATGTGTG ACAGGGGTGA AACTTCCCAA 
GGGGCCAAGT ACACAGGAAG GACTTTGAAC TACCAGAGCC TCCCCCATCG CTCCAGAACA 
GACAACTCCT GGGCACCCTG GTCAGAGACC AACCAGCATA TTGGGACCAG ATTCCTGACT 
ACTCCAGGGT GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC 
CTTCAGGTTC CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC 
ATTGTTCATC CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG 
AATTCAGATC CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA 
GATGATGACT GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA 
GAGGGGTTTC TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC 
CTGCAGCACA GTCAATGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_005269582.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269639.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X29, mRNA.

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CloneID	OHu44068
Clone ID Related Accession (Same CDS sequence)	XM_005269582.2
Accession Version	XM_005269582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG ATTCATCAAA CGTGAGGAAG 
CCTTTGGAAA CCGGGCACCG TTGTTCCAGC TCCTCTTCCC TCCCTGTCAT CCATGACCCT 
TCTGTGTTTC TCCTCGGTCC CCAACTCTAC CTTCCCCAAC CACAGTTCCT GTCCCCAGAT 
GTCCTGATGC CCACCATGGC AGGGGAGCCC AATAGACTCC CAGGAACTTC AAGGAGTGTC 
CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCC AAGGGGCCAA GTACACAGGA 
AGGACTTTGA ACTACCAGAG CCTCCCCCAT CGCTCCAGAA CAGACAACTC CTGGGCACCC 
TGGTCAGAGA CCAACCAGCA TATTGGGACC AGATTCCTGA CTACTCCAGG GTGCAATCCT 
CAACTAACCT ACACTGCCAC ACTACCAGAA AGAAGCAAGG GCCTTCAGGT TCCTCACACT 
CAGTCCTGGA GTGATCTTTT CCATTCACCC TCCCACCCTC CCATTGTTCA TCCTGTGTAC 
CCACCATCTA GCAGTCTTCA TGTACCCCTG AGGTCAGCTT GGAATTCAGA TCCTGTTCCA 
GGGTCCCGAA CCCCTGGTCC TCGAAGAGTA GATATGCCCC CAGATGATGA CTGGAGGCAA 
AGCAGTTATG CCTCCCACTC TGGACACAGG AGAACAGTGG GAGAGGGGTT TCTGTTTGTT 
CTATCAGATG CTCCCAGAAG AGAGCAGATC AGGGCTAGAG TCCTGCAGCA CAGTCAATGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269639.1
CDS	3267..8009
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_005269582.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269582.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGCAATCAGG CTATTTGTAT GCTGGAAAGA 
CGAGAGAAAC CTTCACCAAG CATGTTTGGT GAGCTGCTGC AGAATGCCAG CACCATGGGG 
GATCTGCGGA ACTGTCCAAG CAACTGTGGA GAGAGGATCA GGATTCGCCG TGTGTTGATG 
AATGCTCCAC AGATTATCAC GATTGGGCTG GTATGGGACT CAGACCACTC AGACTTAGCA 
GAAGATGTTA TCCACAGCCT GGGAACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACGGATGACC GGGCCAAGCA ATCTGAACTG TACTTAGTTG GAATGATCTG TTACTATGGC 
AAACATTATT CTACATTCTT TTTTCAAACA AAGATTCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG GCCCAAATGG AAGGATGTGG TGACCAAATG CATCAAGGGG 
CATTATCAGC CCCTGCTGCT GCTTTATGCA GATCCCCAGG GTACCCCAGT TTCCACCCAG 
GACCTGCCTC CCCAAGCTGA GTTCCAGTCA TACAGCAGGA CATGCTACGA CAGTGAAGAT 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCCAAGAA AGGGTCACTG 
ATAGAGCGCA AGAGGAGCTC TGGTCGGGTT AGGAGGAAAG GCGATGAGCC CCAGGCCTCG 
GGATACCACA GTGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGAAA TGCCTCCAAA 
CCATCCAGCA GCACCAACAG GCTGAGAGAT TTTAAAGAGA CAGTCAGCAA TATGATCCAT 
AACAGACCAT CCCTGGCTTC TCAGACCAAT GTAGGCTCTC ACTGCAGGGG CAGAGGAGGA 
GACCAGCCTG ACAAAAAACC TCCTAGGACC CTGCCTTTAC ACTCTCGTGA CTGGGAAATA 
GAGAGTACCA GCAGTGAGTC AAAATCCAGT TCTTCCAGCA AGTATCGTCC CACATGGAGA 
CCCAAACGAG AATCTCTGAA TATTGACAGT ATCTTTAGTA AGGACAAAAG GAAGCACTGT 
GGCTATACCC AGCTTAGCCC CTTTTCTGAG GATTCAGCTA AAGAATTTAT ACCAGATGAA 
CCAAGCAAGC CACCTTCTTA CGACATTAAA TTTGGTGGAC CAAGCCCCCA GTACAAGCGC 
TGGGGCCCAG CACGGCCAGG CTCTCACCTT TTAGAGCAGC ACCCCCGACT AATCCAGCGA 
ATGGAATCTG GCTATGAAAG CAGTGAGAGG AACAGCAGCA GCCCTGTCAG CCTGGATGCA 
GCCCTGCCTG AGAGCTCAAA TGTCTACAGG GATCCAAGTG CTAAGAGATC AGCTGGGTTG 
GTTCCTTCCT GGCGTCATAT CCCAAAGTCG CACAGCAGTA GCATCCTGGA GGTAGACTCC 
ACAGCATCCA TGGGTGGCTG GACAAAGAGT CAGCCTTTCT CTGGTGAGGA GATATCTTCT 
AAAAGTGAAC TGGATGAATT GCAGGAAGAG GTGGCCAGGA GGGCGCAGGA ACAGGAACTT 
CGAAGAAAAC GGGAGAAGGA GTTAGAGGCA GCGAAAGGGT TTAACCCTCA TCCTAGCCGC 
TTCATGGACT TGGATGAACT GCAGAATCAG GGGAGGAGTG ACGGCTTTGA GAGGTCCCTG 
CAAGAGGCAG AGTCAGTGTT TGAAGAGTCA CTACATCTGG AACAGAAAGG AGACTGTGCT 
GCAGCTTTGG CTCTCTGTAA TGAAGCTATC TCTAAACTAA GACTTGCCCT GCATGGTGCC 
AGCTGTAGCA CGCACAGCAG AGCCCTAGTC GATAAGAAGT TGCAAATCAG TATTCGAAAA 
GCACGGAGCC TGCAGGATCG CATGCAGCAG CAGCAATCAC CACAGCAGCC GTCGCAGCCC 
TCAGCCTGCC TCCCAACACA GGCGGGGACT CTCTCTCAGC CAACAAGTGA ACAGCCTATC 
CCGCTCCAAG TATTGTTAAG CCAAGAGGCC CAACTGGAAT CCGGCATGGA TACAGAGTTT 
GGGGCCAGTT CTTTCTTCCA TTCACCTGCT TCCTGCCATG AGTCACACTC ATCACTATCT 
CCAGAGTCAT CTGCCCCACA GCACAGCTCC CCCAGTAGAT CTGCCTTGAA GCTTCTGACT 
TCGGTTGAAG TAGACAACAT TGAACCCTCT GCATTCCACA GGCAAGGTTT ACCTAAAGCA 
CCAGGGTGGA CTGAGAAGAA TTCTCATCAT AGTTGGGAGC CATTGGATGC CCCAGAGGGT 
AAGCTGCAAG GCTCTAGGTG TGACAACAGC AGTTGCAGCA AGCTCCCTCC ACAAGAAGGA 
AGAGGCATTG CTCAAGAACA GCTGTTCCAA GAAAAGAAGG ATCCTGCTAA CCCCTCCCCG 
GTGATGCCTG GAATAGCCAC CTCTGAGAGG GGTGATGAAC ACAGCCTAGG CTGTAGTCCT 
TCAAATTCAT CAGCTCAGCC CAGCCTTCCC CTGTATAGAA CCTGCCACCC CATAATGCCT 
GTTGCTTCTT CATTTGTGCT TCACTGTCCT GATCCTGTGC AGAAAACTAA CCAATGCCTC 
CAAGGCCAAA GCCTCAAAAC TTCATTGACT TTAAAAGTGG ACAGAGGCAG TGAGGAGACC 
TATAGGCCAG AGTTTCCCAG CACAAAGGGG CTTGTCCGTT CTCTGGCTGA GCAGTTCCAG 
AGGATGCAGG GTGTCTCCAT GAGGGATAGT ACAGGTTTCA AGGATAGAAG TTTGTCAGGT 
AGTCTAAGGA AGAACTCTTC CCCTTCTGAT TCTAAGCCTC CTTTCTCACA GGGTCAAGAG 
AAAGGCCACT GGCCATGGGC AAAGCAACAA TCCTCTCTGG AGGGTGGGGA TAGACCACTT 
TCCTGGGAAG AGTCCACTGA ACATTCTTCT CTTGCCTTAA ACTCTGGGCT GCCTAATGGT 
GAAACTTCTA GCGGAGGACA GCCCAGGTTG GCAGAGCCAG ACATATACCA AGAGAAGCTG 
TCCCAAGTGA GAGATGTTAG GTCTAAGGAT CTGGGCAGCA GTACTGACTT GGGGACTTCC 
TTGCCTTTGG ATTCCTGGGT GAATATCACA AGGTTCTGTG ATTCTCAGCT TAAGCATGGG 
GCACCTAGGC CAGGAATGAA GTCCTCCCCT CATGATTCCC ATACGTGTGT AACCTATCCA 
GAGAGAAATC ACATCCTTTT GCATCCACAT TGGAACCAAG ACACAGAGCA GGAGACCTCA 
GAATTGGAGT CTCTGTATCA GGCCAGTCTT CAGGCTTCTC AAGCTGGCTG TTCTGGATGG 
GGGCAGCAGG ATACCGCCTG GCACCCACTT AGCCAAACAG GCTCTGCAGA TGGCATGGGG 
AGGAGGTTGC ACTCAGCCCA TGATCCTGGT CTCTCAAAGA CTTCAACAGC AGAAATGGAG 
CATGGTCTCC ATGAAGCCAG AACAGTGCGT ACTTCTCAGG ATTCATCAAA CGTGAGGAAG 
CCTTTGGAAA CCGGGCACCG TTGTTCCAGC TCCTCTTCCC TCCCTGTCAT CCATGACCCT 
TCTGTGTTTC TCCTCGGTCC CCAACTCTAC CTTCCCCAAC CACAGTTCCT GTCCCCAGAT 
GTCCTGATGC CCACCATGGC AGGGGAGCCC AATAGACTCC CAGGAACTTC AAGGAGTGTC 
CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCC AAGGGGCCAA GTACACAGGA 
AGGACTTTGA ACTACCAGAG CCTCCCCCAT CGCTCCAGAA CAGACAACTC CTGGGCACCC 
TGGTCAGAGA CCAACCAGCA TATTGGGACC AGATTCCTGA CTACTCCAGG GTGCAATCCT 
CAACTAACCT ACACTGCCAC ACTACCAGAA AGAAGCAAGG GCCTTCAGGT TCCTCACACT 
CAGTCCTGGA GTGATCTTTT CCATTCACCC TCCCACCCTC CCATTGTTCA TCCTGTGTAC 
CCACCATCTA GCAGTCTTCA TGTACCCCTG AGGTCAGCTT GGAATTCAGA TCCTGTTCCA 
GGGTCCCGAA CCCCTGGTCC TCGAAGAGTA GATATGCCCC CAGATGATGA CTGGAGGCAA 
AGCAGTTATG CCTCCCACTC TGGACACAGG AGAACAGTGG GAGAGGGGTT TCTGTTTGTT 
CTATCAGATG CTCCCAGAAG AGAGCAGATC AGGGCTAGAG TCCTGCAGCA CAGTCAATGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_011539368.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537670.1 Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.

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CloneID	OHu77043
Clone ID Related Accession (Same CDS sequence)	XM_011539368.2
Accession Version	XM_011539368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GACACCTGAC TGATAGTGAA TGTAATCAGA AACACACATC CAAGAAAGGG 
TCACTGATAG AGCGCAAGAG GAGCTCTGGT CGGGTTAGGA GGAAAGGCGA TGAGCCCCAG 
GCCTCGGGAT ACCACAGTGA AGGAGAAACA CTGAAAGAGA AGCAGGCTCC TAGAAATGCC 
TCCAAACCAT CCAGCAGCAC CAACAGGCTG AGAGATTTTA AAGAGACAGT CAGCAATATG 
ATCCATAACA GACCATCCCT GGCTTCTCAG ACCAATGTAG GCTCTCACTG CAGGGGCAGA 
GGAGGAGACC AGCCTGACAA AAAACCTCCT AGGACCCTGC CTTTACACTC TCGTGACTGG 
GAAATAGAGA GTACCAGCAG TGAGTCAAAA TCCAGTTCTT CCAGCAAGTA TCGTCCCACA 
TGGAGACCCA AACGAGAATC TCTGAATATT GACAGTATCT TTAGTAAGGA CAAAAGGAAG 
CACTGTGGCT ATACCCAGCT TAGCCCCTTT TCTGAGGATT CAGCTAAAGA ATTTATACCA 
GATGAACCAA GCAAGCCACC TTCTTACGAC ATTAAATTTG GTGGACCAAG CCCCCAGTAC 
AAGCGCTGGG GCCCAGCACG GCCAGGCTCT CACCTTTTAG AGCAGCACCC CCGACTAATC 
CAGCGAATGG AATCTGGCTA TGAAAGCAGT GAGAGGAACA GCAGCAGCCC TGTCAGCCTG 
GATGCAGCCC TGCCTGAGAG CTCAAATGTC TACAGGGATC CAAGTGCTAA GAGATCAGCT 
GGGTTGGTTC CTTCCTGGCG TCATATCCCA AAGTCGCACA GCAGTAGCAT CCTGGAGGTA 
GACTCCACAG CATCCATGGG TGGCTGGACA AAGAGTCAGC CTTTCTCTGG TGAGGAGATA 
TCTTCTAAAA GTGAACTGGA TGAATTGCAG GAAGAGGTGG CCAGGAGGGC GCAGGAACAG 
GAACTTCGAA GAAAACGGGA GAAGGAGTTA GAGGCAGCGA AAGGGTTTAA CCCTCATCCT 
AGCCGCTTCA TGGACTTGGA TGAACTGCAG AATCAGGGGA GGAGTGACGG CTTTGAGAGG 
TCCCTGCAAG AGGCAGAGTC AGTGTTTGAA GAGTCACTAC ATCTGGAACA GAAAGGAGAC 
TGTGCTGCAG CTTTGGCTCT CTGTAATGAA GCTATCTCTA AACTAAGACT TGCCCTGCAT 
GGTGCCAGCT GTAGCACGCA CAGCAGAGCC CTAGTCGATA AGAAGTTGCA AATCAGTATT 
CGAAAAGCAC GGAGCCTGCA GGATCGCATG CAGCAGCAGC AATCACCACA GCAGCCGTCG 
CAGCCCTCAG CCTGCCTCCC AACACAGGCG GGGACTCTCT CTCAGCCAAC AAGTGAACAG 
CCTATCCCGC TCCAAGTATT GTTAAGCCAA GAGGCCCAAC TGGAATCCGG CATGGATACA 
GAGTTTGGGG CCAGTTCTTT CTTCCATTCA CCTGCTTCCT GCCATGAGTC ACACTCATCA 
CTATCTCCAG AGTCATCTGC CCCACAGCAC AGCTCCCCCA GTAGATCTGC CTTGAAGCTT 
CTGACTTCGG TTGAAGTAGA CAACATTGAA CCCTCTGCAT TCCACAGGCA AGGTTTACCT 
AAAGCACCAG GGTGGACTGA GAAGAATTCT CATCATAGTT GGGAGCCATT GGATGCCCCA 
GAGGGTAAGC TGCAAGGCTC TAGGTGTGAC AACAGCAGTT GCAGCAAGCT CCCTCCACAA 
GAAGGAAGAG GCATTGCTCA AGAACAGCTG TTCCAAGAAA AGAAGGATCC TGCTAACCCC 
TCCCCGGTGA TGCCTGGAAT AGCCACCTCT GAGAGGGGTG ATGAACACAG CCTAGGCTGT 
AGTCCTTCAA ATTCATCAGC TCAGCCCAGC CTTCCCCTGT ATAGAACCTG CCACCCCATA 
ATGCCTGTTG CTTCTTCATT TGTGCTTCAC TGTCCTGATC CTGTGCAGAA AACTAACCAA 
TGCCTCCAAG GCCAAAGCCT CAAAACTTCA TTGACTTTAA AAGTGGACAG AGGCAGTGAG 
GAGACCTATA GGCCAGAGTT TCCCAGCACA AAGGGGCTTG TCCGTTCTCT GGCTGAGCAG 
TTCCAGAGGA TGCAGGGTGT CTCCATGAGG GATAGTACAG GTTTCAAGGA TAGAAGTTTG 
TCAGGTAGTC TAAGGAAGAA CTCTTCCCCT TCTGATTCTA AGCCTCCTTT CTCACAGGGT 
CAAGAGAAAG GCCACTGGCC ATGGGCAAAG CAACAATCCT CTCTGGAGGG TGGGGATAGA 
CCACTTTCCT GGGAAGAGTC CACTGAACAT TCTTCTCTTG CCTTAAACTC TGGGCTGCCT 
AATGGTGAAA CTTCTAGCGG AGGACAGCCC AGGTTGGCAG AGCCAGACAT ATACCAAGAG 
AAGCTGTCCC AAGTGAGAGA TGTTAGGTCT AAGGATCTGG GCAGCAGTAC TGACTTGGGG 
ACTTCCTTGC CTTTGGATTC CTGGGTGAAT ATCACAAGGT TCTGTGATTC TCAGCTTAAG 
CATGGGGCAC CTAGGCCAGG AATGAAGTCC TCCCCTCATG ATTCCCATAC GTGTGTAACC 
TATCCAGAGA GAAATCACAT CCTTTTGCAT CCACATTGGA ACCAAGACAC AGAGCAGGAG 
ACCTCAGAAT TGGAGTCTCT GTATCAGGCC AGTCTTCAGG CTTCTCAAGC TGGCTGTTCT 
GGATGGGGGC AGCAGGATAC CGCCTGGCAC CCACTTAGCC AAACAGGCTC TGCAGATGGC 
ATGGGGAGGA GGTTGCACTC AGCCCATGAT CCTGGTCTCT CAAAGACTTC AACAGCAGAA 
ATGGAGCATG GTCTCCATGA AGCCAGAACA GTGCGTACTT CTCAGGCTAC ACCTTGCCGA 
GGCCTCAGCA GGGAGTGTGG GGAGGATGAG CAGTACAGTG CAGAGAATTT ACGTCGCATC 
TCACGCAGTC TCAGTGGCAC CGTTGTCTCA GAGAGGGAGG AAGCTCCGGT TTCTTCCCAC 
AGTTTTGATT CATCAAACGT GAGGAAGCCT TTGGAAACCG GGCACCGTTG TTCCAGCTCC 
TCTTCCCTCC CTGTCATCCA TGACCCTTCT GTGTTTCTCC TCGGTCCCCA ACTCTACCTT 
CCCCAACCAC AGTTCCTGTC CCCAGATGTC CTGATGCCCA CCATGGCAGG GGAGCCCAAT 
AGACTCCCAG GAACTTCAAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA 
ACTTCCCAAG GGGCCAAGTA CACAGGAAGG ACTTTGAACT ACCAGAGCCT CCCCCATCGC 
TCCAGAACAG ACAACTCCTG GGCACCCTGG TCAGAGACCA ACCAGCATAT TGGGACCAGA 
TTCCTGACTA CTCCAGGGTG CAATCCTCAA CTAACCTACA CTGCCACACT ACCAGAAAGA 
AGCAAGGGCC TTCAGGTTCC TCACACTCAG TCCTGGAGTG ATCTTTTCCA TTCACCCTCC 
CACCCTCCCA TTGTTCATCC TGTGTACCCA CCATCTAGCA GTCTTCATGT ACCCCTGAGG 
TCAGCTTGGA ATTCAGATCC TGTTCCAGGG TCCCGAACCC CTGGTCCTCG AAGAGTAGAT 
ATGCCCCCAG ATGATGACTG GAGGCAAAGC AGTTATGCCT CCCACTCTGG ACACAGGAGA 
ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA TCAGATGCTC CCAGAAGAGA GCAGATCAGG 
GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537670.1
CDS	3267..8216
Translation	MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLWLSTGQQSGRRSGDSMSFGSGDSNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Target ORF information:

RefSeq Version	XM_011539368.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 54 (USP54), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539368.2

ORF Insert Sequence:

ATGTCTTGGA AGAGAAATTA TTTTTCAGGG GGTCGTGGTA GTGTACAAGG GATGTTTGCA 
CCTCGAAGCT CAACCTCCAT AGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGATATCTT CCGACGTAGC 
TTTAGGCAGC TTACAACTCA CAAGTGCATG GGAGATTCCT GCATCTTTTG CGCTCTCAAG 
GGAATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCATCTGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAGGATGAA CAACGTTTCC AGCTGGGAAT TATGGATGAT 
GCTGCAGAGT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCACATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC CCAACACTGC ATTTCCCATC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTATGTAC TAGCTGTGGT GCCACTTCTG ATCCGCTGCC TTTCATCCAG 
ATGGTACATT ATATCTCCAC CACTTCCCTT TGGCTCAGTA CTGGCCAGCA AAGTGGGAGG 
AGGAGTGGGG ATTCTATGAG CTTTGGATCT GGGGACAGCA ATCAGGCTAT TTGTATGCTG 
GAAAGACGAG AGAAACCTTC ACCAAGCATG TTTGGTGAGC TGCTGCAGAA TGCCAGCACC 
ATGGGGGATC TGCGGAACTG TCCAAGCAAC TGTGGAGAGA GGATCAGGAT TCGCCGTGTG 
TTGATGAATG CTCCACAGAT TATCACGATT GGGCTGGTAT GGGACTCAGA CCACTCAGAC 
TTAGCAGAAG ATGTTATCCA CAGCCTGGGA ACCTGCCTTA AGCTGGGTGA TCTGTTTTTC 
AGAGTGACGG ATGACCGGGC CAAGCAATCT GAACTGTACT TAGTTGGAAT GATCTGTTAC 
TATGGCAAAC ATTATTCTAC ATTCTTTTTT CAAACAAAGA TTCGCAAATG GATGTATTTT 
GATGATGCTC ATGTCAAGGA GATTGGGCCC AAATGGAAGG ATGTGGTGAC CAAATGCATC 
AAGGGGCATT ATCAGCCCCT GCTGCTGCTT TATGCAGATC CCCAGGGTAC CCCAGTTTCC 
ACCCAGGACC TGCCTCCCCA AGCTGAGTTC CAGTCATACA GCAGGACATG CTACGACAGT 
GAAGATTCAG GACACCTGAC TGATAGTGAA TGTAATCAGA AACACACATC CAAGAAAGGG 
TCACTGATAG AGCGCAAGAG GAGCTCTGGT CGGGTTAGGA GGAAAGGCGA TGAGCCCCAG 
GCCTCGGGAT ACCACAGTGA AGGAGAAACA CTGAAAGAGA AGCAGGCTCC TAGAAATGCC 
TCCAAACCAT CCAGCAGCAC CAACAGGCTG AGAGATTTTA AAGAGACAGT CAGCAATATG 
ATCCATAACA GACCATCCCT GGCTTCTCAG ACCAATGTAG GCTCTCACTG CAGGGGCAGA 
GGAGGAGACC AGCCTGACAA AAAACCTCCT AGGACCCTGC CTTTACACTC TCGTGACTGG 
GAAATAGAGA GTACCAGCAG TGAGTCAAAA TCCAGTTCTT CCAGCAAGTA TCGTCCCACA 
TGGAGACCCA AACGAGAATC TCTGAATATT GACAGTATCT TTAGTAAGGA CAAAAGGAAG 
CACTGTGGCT ATACCCAGCT TAGCCCCTTT TCTGAGGATT CAGCTAAAGA ATTTATACCA 
GATGAACCAA GCAAGCCACC TTCTTACGAC ATTAAATTTG GTGGACCAAG CCCCCAGTAC 
AAGCGCTGGG GCCCAGCACG GCCAGGCTCT CACCTTTTAG AGCAGCACCC CCGACTAATC 
CAGCGAATGG AATCTGGCTA TGAAAGCAGT GAGAGGAACA GCAGCAGCCC TGTCAGCCTG 
GATGCAGCCC TGCCTGAGAG CTCAAATGTC TACAGGGATC CAAGTGCTAA GAGATCAGCT 
GGGTTGGTTC CTTCCTGGCG TCATATCCCA AAGTCGCACA GCAGTAGCAT CCTGGAGGTA 
GACTCCACAG CATCCATGGG TGGCTGGACA AAGAGTCAGC CTTTCTCTGG TGAGGAGATA 
TCTTCTAAAA GTGAACTGGA TGAATTGCAG GAAGAGGTGG CCAGGAGGGC GCAGGAACAG 
GAACTTCGAA GAAAACGGGA GAAGGAGTTA GAGGCAGCGA AAGGGTTTAA CCCTCATCCT 
AGCCGCTTCA TGGACTTGGA TGAACTGCAG AATCAGGGGA GGAGTGACGG CTTTGAGAGG 
TCCCTGCAAG AGGCAGAGTC AGTGTTTGAA GAGTCACTAC ATCTGGAACA GAAAGGAGAC 
TGTGCTGCAG CTTTGGCTCT CTGTAATGAA GCTATCTCTA AACTAAGACT TGCCCTGCAT 
GGTGCCAGCT GTAGCACGCA CAGCAGAGCC CTAGTCGATA AGAAGTTGCA AATCAGTATT 
CGAAAAGCAC GGAGCCTGCA GGATCGCATG CAGCAGCAGC AATCACCACA GCAGCCGTCG 
CAGCCCTCAG CCTGCCTCCC AACACAGGCG GGGACTCTCT CTCAGCCAAC AAGTGAACAG 
CCTATCCCGC TCCAAGTATT GTTAAGCCAA GAGGCCCAAC TGGAATCCGG CATGGATACA 
GAGTTTGGGG CCAGTTCTTT CTTCCATTCA CCTGCTTCCT GCCATGAGTC ACACTCATCA 
CTATCTCCAG AGTCATCTGC CCCACAGCAC AGCTCCCCCA GTAGATCTGC CTTGAAGCTT 
CTGACTTCGG TTGAAGTAGA CAACATTGAA CCCTCTGCAT TCCACAGGCA AGGTTTACCT 
AAAGCACCAG GGTGGACTGA GAAGAATTCT CATCATAGTT GGGAGCCATT GGATGCCCCA 
GAGGGTAAGC TGCAAGGCTC TAGGTGTGAC AACAGCAGTT GCAGCAAGCT CCCTCCACAA 
GAAGGAAGAG GCATTGCTCA AGAACAGCTG TTCCAAGAAA AGAAGGATCC TGCTAACCCC 
TCCCCGGTGA TGCCTGGAAT AGCCACCTCT GAGAGGGGTG ATGAACACAG CCTAGGCTGT 
AGTCCTTCAA ATTCATCAGC TCAGCCCAGC CTTCCCCTGT ATAGAACCTG CCACCCCATA 
ATGCCTGTTG CTTCTTCATT TGTGCTTCAC TGTCCTGATC CTGTGCAGAA AACTAACCAA 
TGCCTCCAAG GCCAAAGCCT CAAAACTTCA TTGACTTTAA AAGTGGACAG AGGCAGTGAG 
GAGACCTATA GGCCAGAGTT TCCCAGCACA AAGGGGCTTG TCCGTTCTCT GGCTGAGCAG 
TTCCAGAGGA TGCAGGGTGT CTCCATGAGG GATAGTACAG GTTTCAAGGA TAGAAGTTTG 
TCAGGTAGTC TAAGGAAGAA CTCTTCCCCT TCTGATTCTA AGCCTCCTTT CTCACAGGGT 
CAAGAGAAAG GCCACTGGCC ATGGGCAAAG CAACAATCCT CTCTGGAGGG TGGGGATAGA 
CCACTTTCCT GGGAAGAGTC CACTGAACAT TCTTCTCTTG CCTTAAACTC TGGGCTGCCT 
AATGGTGAAA CTTCTAGCGG AGGACAGCCC AGGTTGGCAG AGCCAGACAT ATACCAAGAG 
AAGCTGTCCC AAGTGAGAGA TGTTAGGTCT AAGGATCTGG GCAGCAGTAC TGACTTGGGG 
ACTTCCTTGC CTTTGGATTC CTGGGTGAAT ATCACAAGGT TCTGTGATTC TCAGCTTAAG 
CATGGGGCAC CTAGGCCAGG AATGAAGTCC TCCCCTCATG ATTCCCATAC GTGTGTAACC 
TATCCAGAGA GAAATCACAT CCTTTTGCAT CCACATTGGA ACCAAGACAC AGAGCAGGAG 
ACCTCAGAAT TGGAGTCTCT GTATCAGGCC AGTCTTCAGG CTTCTCAAGC TGGCTGTTCT 
GGATGGGGGC AGCAGGATAC CGCCTGGCAC CCACTTAGCC AAACAGGCTC TGCAGATGGC 
ATGGGGAGGA GGTTGCACTC AGCCCATGAT CCTGGTCTCT CAAAGACTTC AACAGCAGAA 
ATGGAGCATG GTCTCCATGA AGCCAGAACA GTGCGTACTT CTCAGGCTAC ACCTTGCCGA 
GGCCTCAGCA GGGAGTGTGG GGAGGATGAG CAGTACAGTG CAGAGAATTT ACGTCGCATC 
TCACGCAGTC TCAGTGGCAC CGTTGTCTCA GAGAGGGAGG AAGCTCCGGT TTCTTCCCAC 
AGTTTTGATT CATCAAACGT GAGGAAGCCT TTGGAAACCG GGCACCGTTG TTCCAGCTCC 
TCTTCCCTCC CTGTCATCCA TGACCCTTCT GTGTTTCTCC TCGGTCCCCA ACTCTACCTT 
CCCCAACCAC AGTTCCTGTC CCCAGATGTC CTGATGCCCA CCATGGCAGG GGAGCCCAAT 
AGACTCCCAG GAACTTCAAG GAGTGTCCAG CAGTTTCTGG CTATGTGTGA CAGGGGTGAA 
ACTTCCCAAG GGGCCAAGTA CACAGGAAGG ACTTTGAACT ACCAGAGCCT CCCCCATCGC 
TCCAGAACAG ACAACTCCTG GGCACCCTGG TCAGAGACCA ACCAGCATAT TGGGACCAGA 
TTCCTGACTA CTCCAGGGTG CAATCCTCAA CTAACCTACA CTGCCACACT ACCAGAAAGA 
AGCAAGGGCC TTCAGGTTCC TCACACTCAG TCCTGGAGTG ATCTTTTCCA TTCACCCTCC 
CACCCTCCCA TTGTTCATCC TGTGTACCCA CCATCTAGCA GTCTTCATGT ACCCCTGAGG 
TCAGCTTGGA ATTCAGATCC TGTTCCAGGG TCCCGAACCC CTGGTCCTCG AAGAGTAGAT 
ATGCCCCCAG ATGATGACTG GAGGCAAAGC AGTTATGCCT CCCACTCTGG ACACAGGAGA 
ACAGTGGGAG AGGGGTTTCT GTTTGTTCTA TCAGATGCTC CCAGAAGAGA GCAGATCAGG 
GCTAGAGTCC TGCAGCACAG TCAATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The deubiquitinase USP54 is overexpressed in colorectal cancer stem cells and promotes intestinal tumorigenesis.
Oncotarget7(46)74427-74434(2016 Nov)
Fraile JM,Campos-Iglesias D,Rodr?guez F,Espa?ol Y,Freije JM

Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases.
Biochemical and biophysical research communications314(1)54-62(2004 Jan)
Quesada V,D?az-Perales A,Guti?rrez-Fern?ndez A,Garabaya C,Cal S,L?pez-Ot?n C

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USP54 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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