RAPGEF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF2
Entrez Gene ID	100139484
Full Name	Rap guanine nucleotide exchange factor 2
Synonyms	PDZ-GEF1,RA-GEF-1,nRap GEP
General protein information	Preferred Names Rap guanine nucleotide exchange factor 2 Names rap guanine nucleotide exchange factor 2 CNrasGEF PDZ domain-containing guanine nucleotide exchange factor 1 Rap guanine nucleotide exchange factor (GEF) 2 cyclic nucleotide ras GEF neural RAP guanine nucleotide exchange protein ras/Rap1-associating GEF-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475408.1	XP_015330894.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_015475409.1	XP_015330895.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_010813791.2	XP_010812093.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_010813792.2	XP_010812094.1	rap guanine nucleotide exchange factor 2 isoform X7
XM_015475412.1	XP_015330898.1	rap guanine nucleotide exchange factor 2 isoform X9
XM_015475415.1	XP_015330901.1	rap guanine nucleotide exchange factor 2 isoform X11
XM_024976981.1	XP_024832749.1	rap guanine nucleotide exchange factor 2 isoform X12
XM_005217619.3	XP_005217676.1	rap guanine nucleotide exchange factor 2 isoform X27
NM_001191326.1	NP_001178255.1	rap guanine nucleotide exchange factor 2
XM_015475414.1	XP_015330900.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_024976970.1	XP_024832738.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_024976972.1	XP_024832740.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_024976975.1	XP_024832743.1	rap guanine nucleotide exchange factor 2 isoform X7
XM_024976976.1	XP_024832744.1	rap guanine nucleotide exchange factor 2 isoform X8
XM_024976977.1	XP_024832745.1	rap guanine nucleotide exchange factor 2 isoform X9
XM_024976978.1	XP_024832746.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_024976979.1	XP_024832747.1	rap guanine nucleotide exchange factor 2 isoform X11
XM_024976987.1	XP_024832755.1	rap guanine nucleotide exchange factor 2 isoform X18
XM_024976971.1	XP_024832739.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_024976974.1	XP_024832742.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_024976986.1	XP_024832754.1	rap guanine nucleotide exchange factor 2 isoform X17
XM_015475413.2	XP_015330899.1	rap guanine nucleotide exchange factor 2 isoform X22
XM_015475414.2	XP_015330900.1	rap guanine nucleotide exchange factor 2 isoform X22
XM_024976992.1	XP_024832760.1	rap guanine nucleotide exchange factor 2 isoform X25
XM_015475411.1	XP_015330897.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_010813793.2	XP_010812095.1	rap guanine nucleotide exchange factor 2 isoform X8
XM_015475413.1	XP_015330899.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_024976973.1	XP_024832741.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_024976982.1	XP_024832750.1	rap guanine nucleotide exchange factor 2 isoform X13
XM_024976988.1	XP_024832756.1	rap guanine nucleotide exchange factor 2 isoform X19
XM_024976989.1	XP_024832757.1	rap guanine nucleotide exchange factor 2 isoform X21
XM_024976991.1	XP_024832759.1	rap guanine nucleotide exchange factor 2 isoform X23
XM_024976993.1	XP_024832761.1	rap guanine nucleotide exchange factor 2 isoform X26
XM_015475406.1	XP_015330892.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_015475407.1	XP_015330893.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_024976969.1	XP_024832737.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_024976983.1	XP_024832751.1	rap guanine nucleotide exchange factor 2 isoform X14
XM_024976984.1	XP_024832752.1	rap guanine nucleotide exchange factor 2 isoform X15
XM_024976985.1	XP_024832753.1	rap guanine nucleotide exchange factor 2 isoform X16
XM_015475412.2	XP_015330898.1	rap guanine nucleotide exchange factor 2 isoform X20
XM_015475415.2	XP_015330901.1	rap guanine nucleotide exchange factor 2 isoform X24

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAPGEF2	NP_055062.1
	RAPGEF2	XP_001093090.2
Mus musculus (house mouse)	Rapgef2	NP_001093094.2
Rattus norvegicus (Norway rat)	Rapgef2	NP_001101154.1
Gallus gallus (chicken)	RAPGEF2	XP_004940966.1
Xenopus tropicalis (tropical clawed frog)	rapgef2	NP_001120522.1
Pan troglodytes (chimpanzee)	RAPGEF2	XP_001147382.2
Canis lupus familiaris (dog)	RAPGEF2	XP_005629442.1
Danio rerio (zebrafish)	rapgef2	XP_005173396.1
Bos taurus (cattle)	RAPGEF2	NP_001178255.1

Gene Ontology
Bibliography

Related articles in PubMed

Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.
Emery AC, Eiden MV, Mustafa T, Eiden LE
Science signaling6(281)ra51(2013 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification of a novel beta-catenin-interacting protein.
Kawajiri A, Itoh N, Fukata M, Nakagawa M, Yamaga M, Iwamatsu A, Kaibuchi K
Biochemical and biophysical research communications273(2)712-7(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Rapgef2 is a cAMP-binding protein present in chromaffin cells.
Title: Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa224116	XM_024976969.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224117	XM_024976970.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224118	XM_024976971.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224119	XM_024976972.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224120	XM_024976973.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224121	XM_024976974.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224122	XM_024976975.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224123	XM_024976976.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224124	XM_024976977.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa224125	XM_024976978.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224126	XM_024976979.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224127	XM_024976981.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224128	XM_024976982.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224129	XM_024976983.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224130	XM_024976984.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224131	XM_024976985.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224132	XM_024976986.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224133	XM_024976987.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224134	XM_024976988.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224135	XM_024976989.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224136	XM_024976992.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa224137	XM_024976993.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa58990	XM_024976991.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93151	XM_015475406.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93152	XM_015475407.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93153	XM_015475408.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93154	XM_015475409.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93155	XM_015475411.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93156	XM_015475412.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93157	XM_015475414.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93157	XM_015475413.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93158	XM_015475415.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa01279	NM_001191326.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27714	XM_005217619.3 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa58983	XM_010813791.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa58984	XM_010813792.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa58985	XM_010813793.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa93157	XM_015475414.1	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93157	XM_015475413.1	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93156	XM_015475412.1	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93158	XM_015475415.1	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF2 ( XM_024976969.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832737.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OBa224116
Clone ID Related Accession (Same CDS sequence)	XM_024976969.1
Accession Version	XM_024976969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGCAGAAG ATGAACAAGT GTCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832737.1
CDS	77..5149
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976969.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976969.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGCAGAAG ATGAACAAGT GTCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976970.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832738.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OBa224117
Clone ID Related Accession (Same CDS sequence)	XM_024976970.1
Accession Version	XM_024976970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832738.1
CDS	77..5146
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976970.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976970.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976971.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832739.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OBa224118
Clone ID Related Accession (Same CDS sequence)	XM_024976971.1
Accession Version	XM_024976971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832739.1
CDS	77..5146
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976971.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976971.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976972.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832740.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OBa224119
Clone ID Related Accession (Same CDS sequence)	XM_024976972.1
Accession Version	XM_024976972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832740.1
CDS	77..5143
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976972.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976972.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976973.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832741.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa224120
Clone ID Related Accession (Same CDS sequence)	XM_024976973.1
Accession Version	XM_024976973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGCTTCC TGCAGATTTC 
ACAAAGCTAC ACCTCACTGA CAGTCTCCAC CCACAGGTGA CCCACGTCTC CTCCAGCCAC 
TCAGGATGTA GCATCACTAG TGATTCTGGA AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAGAGTG AGGCTGGTGA TATGGACCTT AGTGGGTTGC CAGAAACAGC AGTGGACTCT 
GAGGACGATG ATGATGAAGA GGACATCGAG AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC 
ATCGTGAGAG ACTGCCTGGA GAAGGACCCC ATTGATAGGA CAGACGATGA CATTGAACAA 
CTTCTGGAAT TCATGCACCA GTTGCCTGCT TTTGCCAACA TGACGATGTC GGTGAGGCGG 
GAGCTGTGTG CTGTGATGGT GTTCGCTGTG GTGGAAAGAG CTGGGACCAT CGTGTTAAAC 
GACGGTGAAG AGCTGGACTC ATGGTCAGTG ATCCTGAATG GTTCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTCTGTATG GGAAATAGTT TCGGTGTCTC CCCCACCATG 
GACAAGGAGT ACATGAAAGG TGTGATGAGG ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATCCTC AACCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATCGT CATGGTGAAG GAACACCGAG AGCTTGATCG GACTGGAACA 
AGAAAGGGGC ACATTGTCAT CAAGGGCACC TCAGAAAGGT TAACGATGCA TTTGGTGGAA 
GAACATTCAG TGGTGGATCC AACGTTCATA GAAGACTTCC TGTTGACCTA TAGGACTTTT 
CTCTGTAGCC CGATGGAAGT GGGCAAAAAG TTGTTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACGCGGGT AGTATTATTG TGGGTGAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTCTAGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ATCTAAGGCT GTTAAATATT GCATGTGCAG CTAAAGCAAA AAGAAGACTG 
ATGACGCTAA CGAAACCAGC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCA 
GAGAAAGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG CTAGCAAAGC AACAGAAGCG 
GGCTTGAAAC GAGGGGATCA GATACTAGAA GTAAATGGTC AAAACTTTGA AAATATTCAG 
CTATCAAAAG CCATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTATCAG AAGAGAAGAG AAATGGTGCC 
CCTCACCTCC CTAAAATTGG AGACATCAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTC 
GCAGTAGATG TAGAGCAAGT GATAGGACTT GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC 
ACTGTTGGGG GAAGGAACAA GCTCAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATATAATGA TATCGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CTAAGCACAT CCCAACTGCA CTGCCTGTCA GCGGAACCTT ATCGTCCAGT 
AATCCTGACT TACTGCAGTC ACACCATCGC ATTTTAGACT TCAGTGCTAC TCCTGATTTG 
CCAGATCAAG TGCTAAGGGT TTTCAAGGCT GACCAGCAAA GCCGATACAT CATGATCAGT 
AAGGACACTA CGGCGAAGGA AGTGGTCGTT CAGGCCATCC GGGAGTTTGC CGTGACCGCC 
ACCCCAGATC AGTACTCGCT CTGTGAAGTC TCTGTCACAC CAGAGGGAGT GATCAAGCAA 
AGAAGGCTTC CAGATCAGCT TTCCAAACTT GCTGACAGAA TACAGCTGAG CGGAAGGTAT 
TACTTGAAAA ACAACATGGA GACGGAAACC CTTTGCTCGG ATGAGGATGC ACAGGAGCTG 
CTGCGAGAGA GCCAGATCTC ACTGCTGCAG CTCAGCACCG TGGAGGTGGC CACGCAGCTC 
TCCATGCGCA ACTTCGAGCT TTTCCGCAAC ATCGAGCCCA CTGAGTATAT CGATGACTTA 
TTCAAGCTTA AATCAAAAAC CAGCTGTGCC AACCTGAAGA CATTCGAGGA AGTCATTAAC 
CAGGAGACGT TTTGGGTAGC ATCTGAGATT CTGCGGGAGA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCT CTGCACTGTA GGGAGTGCAA GAATTTTAAC 
TCCATGTTTG CGATCATCAG TGGCCTAAAC CTGGCGCCGG TGGCAAGACT GCGAACTACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAG CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATACCGCAAT GTCCTCAACA GTCAGAACCT GCAGCCTCCT 
ATCATCCCCC TGTTCCCAGT GATCAAGAAG GATCTTACGT TCCTTCACGA AGGGAATGAC 
TCCAAGGTTG ATGGGCTGGT CAATTTTGAG AAGCTAAGAA TGATTGCAAA GGAAATACGT 
CACGTTGGCC GAATGGCCTC TGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC CAGGGTAGTA CAAACGCAAC CGTGCTAGAT 
GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG GTGCGTCGTA GCTCCTTTCT CAATGCCAAA 
AAGCTATATG AAGATGCCCA GATGGCTCGC AAAGTGAAGC AGTACCTGTC CAATCTGGAG 
CTCGAGATGG ATGAGGAGAG TCTTCAGACG CTATCCCTGC AGTGTGAGCC GGCCACCAAC 
ACACTGCCTA AGAATCCCAC TGACAAAAAG CCTGTCAAAT CGGAGACCTC CCCAGTGGCC 
CCGAGAGCGG GGTTGCAGCC GAAAGCACAG CCGCAGCCCC AGCCGCCGCA GCCGCCACAC 
AAACTCAACC AGGGACTGCA GGTTCCCGCC GTGTCCCTTT ACCCTTCACG GAAGAAAGTT 
CCCGTAAAGG ACCTCCCACC TTTCGGCATC AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC CATAGGAGGC CAGCAGAAGA CACGATATCC 
AACACGTCTT CCCAGCTCTC CTCTCCGCCC ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT 
GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC TCCGGGCAGT TGGATTTAAC CAGCTCCTCC 
TCTTCTCTTG GAAGTGAGAA CAGGAACAAG AATAACAATG CACCACGGAC CTATGGGATA 
GGCTACACTT TGGCTCCGAG TGGCACCGTG GATAACTTCT CAGATTCCGG CCACAGTGAA 
ATCTCCTCAC GATCCAGTAT CGTCAGCAAC TCCTCCTTCG ATTCATTGCC CGTCTCGCTG 
CCTGACGAGC GGCGCCAGAG GCCGTCAGTC AGCATCGTGG AGACCAGCCT GGCCTCGGGC 
CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT GACCAGTATA GCCTGGGTTC CTGTGCACCA 
CTGTCTGAGA GCAGGGGCCT ATATGCTGCA GCCACCGTGA TCTCTTCCCC CAGCACGGAG 
GAGCTTTCCC AGGACCAGGG GGACCGAGCC TCCCTGGACG CTGCTGACAG TGGCCGGGGG 
AGCTGGACGT CGTGCTCGAG CGGCTCCCAT GATAACATCC AGACCATCCA GCACCAGAGG 
AGCTGGGAGA CGCTCCCCTT TGGGCACACT CACTTTGATT ACCCAGGGGA TGCTGCAGGG 
CTGTGGGCCT CGAGCAGCCA TATGGACCAA ATCATGTTTC CCGACCACAG TGCAAAGTAC 
AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC GATCAGGCCC AGTCCCGGGC CAGCTGGGCC 
TCGTCCACAG GGTACTGGGG AGAGGACTCG GAAGGGGACA CGGGCACCAT CAAGAGAAGG 
GGGGGTAAGG ACGTTTCCCT TGACGCTGAC AGCAGCAGCA TGACAGCCGT GACCGCAGAG 
GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC ATAGCCGTGA CACCCAGTGC TGCCAAGGGG 
CTCATCGCAG CCAGGAAGGA GGGCCGCTAC CGGGAGCCGC CGCCCACTCC ACCAGGCTAC 
GTGGGCATCC CCATCACTGA CTTCCCCGAG GCCCACCCGC ACCCGGCCCG GAAACCGCCG 
GACTACACTG TGGCGCTGCA GCGGTCCAGG ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC 
CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG CAGTGGCACC GGCCTGGTGA TGGTGACCCA 
CGCGCCGGCC CCTGTGCACC CCCGGGCCTC ACCGCCGAGG AGGACGCAGA AGATGAACAA 
GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832741.1
CDS	77..5131
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976973.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976973.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGCTTCC TGCAGATTTC 
ACAAAGCTAC ACCTCACTGA CAGTCTCCAC CCACAGGTGA CCCACGTCTC CTCCAGCCAC 
TCAGGATGTA GCATCACTAG TGATTCTGGA AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAGAGTG AGGCTGGTGA TATGGACCTT AGTGGGTTGC CAGAAACAGC AGTGGACTCT 
GAGGACGATG ATGATGAAGA GGACATCGAG AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC 
ATCGTGAGAG ACTGCCTGGA GAAGGACCCC ATTGATAGGA CAGACGATGA CATTGAACAA 
CTTCTGGAAT TCATGCACCA GTTGCCTGCT TTTGCCAACA TGACGATGTC GGTGAGGCGG 
GAGCTGTGTG CTGTGATGGT GTTCGCTGTG GTGGAAAGAG CTGGGACCAT CGTGTTAAAC 
GACGGTGAAG AGCTGGACTC ATGGTCAGTG ATCCTGAATG GTTCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTCTGTATG GGAAATAGTT TCGGTGTCTC CCCCACCATG 
GACAAGGAGT ACATGAAAGG TGTGATGAGG ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATCCTC AACCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATCGT CATGGTGAAG GAACACCGAG AGCTTGATCG GACTGGAACA 
AGAAAGGGGC ACATTGTCAT CAAGGGCACC TCAGAAAGGT TAACGATGCA TTTGGTGGAA 
GAACATTCAG TGGTGGATCC AACGTTCATA GAAGACTTCC TGTTGACCTA TAGGACTTTT 
CTCTGTAGCC CGATGGAAGT GGGCAAAAAG TTGTTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACGCGGGT AGTATTATTG TGGGTGAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTCTAGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ATCTAAGGCT GTTAAATATT GCATGTGCAG CTAAAGCAAA AAGAAGACTG 
ATGACGCTAA CGAAACCAGC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCA 
GAGAAAGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG CTAGCAAAGC AACAGAAGCG 
GGCTTGAAAC GAGGGGATCA GATACTAGAA GTAAATGGTC AAAACTTTGA AAATATTCAG 
CTATCAAAAG CCATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTATCAG AAGAGAAGAG AAATGGTGCC 
CCTCACCTCC CTAAAATTGG AGACATCAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTC 
GCAGTAGATG TAGAGCAAGT GATAGGACTT GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC 
ACTGTTGGGG GAAGGAACAA GCTCAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATATAATGA TATCGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CTAAGCACAT CCCAACTGCA CTGCCTGTCA GCGGAACCTT ATCGTCCAGT 
AATCCTGACT TACTGCAGTC ACACCATCGC ATTTTAGACT TCAGTGCTAC TCCTGATTTG 
CCAGATCAAG TGCTAAGGGT TTTCAAGGCT GACCAGCAAA GCCGATACAT CATGATCAGT 
AAGGACACTA CGGCGAAGGA AGTGGTCGTT CAGGCCATCC GGGAGTTTGC CGTGACCGCC 
ACCCCAGATC AGTACTCGCT CTGTGAAGTC TCTGTCACAC CAGAGGGAGT GATCAAGCAA 
AGAAGGCTTC CAGATCAGCT TTCCAAACTT GCTGACAGAA TACAGCTGAG CGGAAGGTAT 
TACTTGAAAA ACAACATGGA GACGGAAACC CTTTGCTCGG ATGAGGATGC ACAGGAGCTG 
CTGCGAGAGA GCCAGATCTC ACTGCTGCAG CTCAGCACCG TGGAGGTGGC CACGCAGCTC 
TCCATGCGCA ACTTCGAGCT TTTCCGCAAC ATCGAGCCCA CTGAGTATAT CGATGACTTA 
TTCAAGCTTA AATCAAAAAC CAGCTGTGCC AACCTGAAGA CATTCGAGGA AGTCATTAAC 
CAGGAGACGT TTTGGGTAGC ATCTGAGATT CTGCGGGAGA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCT CTGCACTGTA GGGAGTGCAA GAATTTTAAC 
TCCATGTTTG CGATCATCAG TGGCCTAAAC CTGGCGCCGG TGGCAAGACT GCGAACTACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAG CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATACCGCAAT GTCCTCAACA GTCAGAACCT GCAGCCTCCT 
ATCATCCCCC TGTTCCCAGT GATCAAGAAG GATCTTACGT TCCTTCACGA AGGGAATGAC 
TCCAAGGTTG ATGGGCTGGT CAATTTTGAG AAGCTAAGAA TGATTGCAAA GGAAATACGT 
CACGTTGGCC GAATGGCCTC TGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC CAGGGTAGTA CAAACGCAAC CGTGCTAGAT 
GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG GTGCGTCGTA GCTCCTTTCT CAATGCCAAA 
AAGCTATATG AAGATGCCCA GATGGCTCGC AAAGTGAAGC AGTACCTGTC CAATCTGGAG 
CTCGAGATGG ATGAGGAGAG TCTTCAGACG CTATCCCTGC AGTGTGAGCC GGCCACCAAC 
ACACTGCCTA AGAATCCCAC TGACAAAAAG CCTGTCAAAT CGGAGACCTC CCCAGTGGCC 
CCGAGAGCGG GGTTGCAGCC GAAAGCACAG CCGCAGCCCC AGCCGCCGCA GCCGCCACAC 
AAACTCAACC AGGGACTGCA GGTTCCCGCC GTGTCCCTTT ACCCTTCACG GAAGAAAGTT 
CCCGTAAAGG ACCTCCCACC TTTCGGCATC AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC CATAGGAGGC CAGCAGAAGA CACGATATCC 
AACACGTCTT CCCAGCTCTC CTCTCCGCCC ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT 
GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC TCCGGGCAGT TGGATTTAAC CAGCTCCTCC 
TCTTCTCTTG GAAGTGAGAA CAGGAACAAG AATAACAATG CACCACGGAC CTATGGGATA 
GGCTACACTT TGGCTCCGAG TGGCACCGTG GATAACTTCT CAGATTCCGG CCACAGTGAA 
ATCTCCTCAC GATCCAGTAT CGTCAGCAAC TCCTCCTTCG ATTCATTGCC CGTCTCGCTG 
CCTGACGAGC GGCGCCAGAG GCCGTCAGTC AGCATCGTGG AGACCAGCCT GGCCTCGGGC 
CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT GACCAGTATA GCCTGGGTTC CTGTGCACCA 
CTGTCTGAGA GCAGGGGCCT ATATGCTGCA GCCACCGTGA TCTCTTCCCC CAGCACGGAG 
GAGCTTTCCC AGGACCAGGG GGACCGAGCC TCCCTGGACG CTGCTGACAG TGGCCGGGGG 
AGCTGGACGT CGTGCTCGAG CGGCTCCCAT GATAACATCC AGACCATCCA GCACCAGAGG 
AGCTGGGAGA CGCTCCCCTT TGGGCACACT CACTTTGATT ACCCAGGGGA TGCTGCAGGG 
CTGTGGGCCT CGAGCAGCCA TATGGACCAA ATCATGTTTC CCGACCACAG TGCAAAGTAC 
AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC GATCAGGCCC AGTCCCGGGC CAGCTGGGCC 
TCGTCCACAG GGTACTGGGG AGAGGACTCG GAAGGGGACA CGGGCACCAT CAAGAGAAGG 
GGGGGTAAGG ACGTTTCCCT TGACGCTGAC AGCAGCAGCA TGACAGCCGT GACCGCAGAG 
GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC ATAGCCGTGA CACCCAGTGC TGCCAAGGGG 
CTCATCGCAG CCAGGAAGGA GGGCCGCTAC CGGGAGCCGC CGCCCACTCC ACCAGGCTAC 
GTGGGCATCC CCATCACTGA CTTCCCCGAG GCCCACCCGC ACCCGGCCCG GAAACCGCCG 
GACTACACTG TGGCGCTGCA GCGGTCCAGG ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC 
CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG CAGTGGCACC GGCCTGGTGA TGGTGACCCA 
CGCGCCGGCC CCTGTGCACC CCCGGGCCTC ACCGCCGAGG AGGACGCAGA AGATGAACAA 
GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976974.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832742.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa224121
Clone ID Related Accession (Same CDS sequence)	XM_024976974.1
Accession Version	XM_024976974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGTGGCAGT 
GGCAGTCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGATT TAACCAGCTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGGAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACTTTGGCTC CGAGTGGCAC CGTGGATAAC TTCTCAGATT CCGGCCACAG TGAAATCTCC 
TCACGATCCA GTATCGTCAG CAACTCCTCC TTCGATTCAT TGCCCGTCTC GCTGCCTGAC 
GAGCGGCGCC AGAGGCCGTC AGTCAGCATC GTGGAGACCA GCCTGGCCTC GGGCCGGCTG 
GAGAGGCGGC CGGCGGTGGA GCCTGACCAG TATAGCCTGG GTTCCTGTGC ACCACTGTCT 
GAGAGCAGGG GCCTATATGC TGCAGCCACC GTGATCTCTT CCCCCAGCAC GGAGGAGCTT 
TCCCAGGACC AGGGGGACCG AGCCTCCCTG GACGCTGCTG ACAGTGGCCG GGGGAGCTGG 
ACGTCGTGCT CGAGCGGCTC CCATGATAAC ATCCAGACCA TCCAGCACCA GAGGAGCTGG 
GAGACGCTCC CCTTTGGGCA CACTCACTTT GATTACCCAG GGGATGCTGC AGGGCTGTGG 
GCCTCGAGCA GCCATATGGA CCAAATCATG TTTCCCGACC ACAGTGCAAA GTACAGCAGG 
CAGAGTCAGA GCCGAGAGAG TCTCGATCAG GCCCAGTCCC GGGCCAGCTG GGCCTCGTCC 
ACAGGGTACT GGGGAGAGGA CTCGGAAGGG GACACGGGCA CCATCAAGAG AAGGGGGGGT 
AAGGACGTTT CCCTTGACGC TGACAGCAGC AGCATGACAG CCGTGACCGC AGAGGAAGCC 
AAACCTGCCG CCATGGCTGC CCACATAGCC GTGACACCCA GTGCTGCCAA GGGGCTCATC 
GCAGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832742.1
CDS	77..5125
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976974.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976974.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGTGGCAGT 
GGCAGTCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGATT TAACCAGCTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGGAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACTTTGGCTC CGAGTGGCAC CGTGGATAAC TTCTCAGATT CCGGCCACAG TGAAATCTCC 
TCACGATCCA GTATCGTCAG CAACTCCTCC TTCGATTCAT TGCCCGTCTC GCTGCCTGAC 
GAGCGGCGCC AGAGGCCGTC AGTCAGCATC GTGGAGACCA GCCTGGCCTC GGGCCGGCTG 
GAGAGGCGGC CGGCGGTGGA GCCTGACCAG TATAGCCTGG GTTCCTGTGC ACCACTGTCT 
GAGAGCAGGG GCCTATATGC TGCAGCCACC GTGATCTCTT CCCCCAGCAC GGAGGAGCTT 
TCCCAGGACC AGGGGGACCG AGCCTCCCTG GACGCTGCTG ACAGTGGCCG GGGGAGCTGG 
ACGTCGTGCT CGAGCGGCTC CCATGATAAC ATCCAGACCA TCCAGCACCA GAGGAGCTGG 
GAGACGCTCC CCTTTGGGCA CACTCACTTT GATTACCCAG GGGATGCTGC AGGGCTGTGG 
GCCTCGAGCA GCCATATGGA CCAAATCATG TTTCCCGACC ACAGTGCAAA GTACAGCAGG 
CAGAGTCAGA GCCGAGAGAG TCTCGATCAG GCCCAGTCCC GGGCCAGCTG GGCCTCGTCC 
ACAGGGTACT GGGGAGAGGA CTCGGAAGGG GACACGGGCA CCATCAAGAG AAGGGGGGGT 
AAGGACGTTT CCCTTGACGC TGACAGCAGC AGCATGACAG CCGTGACCGC AGAGGAAGCC 
AAACCTGCCG CCATGGCTGC CCACATAGCC GTGACACCCA GTGCTGCCAA GGGGCTCATC 
GCAGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976975.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832743.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

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CloneID	OBa224122
Clone ID Related Accession (Same CDS sequence)	XM_024976975.1
Accession Version	XM_024976975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832743.1
CDS	77..5101
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976975.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976975.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976976.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832744.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

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CloneID	OBa224123
Clone ID Related Accession (Same CDS sequence)	XM_024976976.1
Accession Version	XM_024976976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832744.1
CDS	77..5101
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976976.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976976.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976977.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832745.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

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CloneID	OBa224124
Clone ID Related Accession (Same CDS sequence)	XM_024976977.1
Accession Version	XM_024976977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832745.1
CDS	1..5022
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976977.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976977.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976978.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832746.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

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CloneID	OBa224125
Clone ID Related Accession (Same CDS sequence)	XM_024976978.1
Accession Version	XM_024976978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATTT TGGCAAGCGT TCTGCAGGAA GCTTTAGGCG TGGCTGTGAA 
TGCATTGTGT TAGAGCCTTC TGAAATGATT GTGGTAGACT ATATGGATGA AAATGAAGAA 
TATTTTCAGC GTCAAGCTTC TCATCGACAG TCTCGAAGGA GATTTAGAAA AATCAACCAG 
AAAGGGGAAA GACAAACTAT TATTGACACT GTGGATCCTT ATCCTGTGGG CAAACCACCT 
TTGCCTAGAG GCTATCATAC GGAATGCACT AAATCTCAGC TTCCTGCAGA TTTCACAAAG 
CTACACCTCA CTGACAGTCT CCACCCACAG GTGACCCACG TCTCCTCCAG CCACTCAGGA 
TGTAGCATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAG 
AGTGAGGCTG GTGATATGGA CCTTAGTGGG TTGCCAGAAA CAGCAGTGGA CTCTGAGGAC 
GATGATGATG AAGAGGACAT CGAGAGGGCC TCAGACCCTC TAATGAGCAG AGACATCGTG 
AGAGACTGCC TGGAGAAGGA CCCCATTGAT AGGACAGACG ATGACATTGA ACAACTTCTG 
GAATTCATGC ACCAGTTGCC TGCTTTTGCC AACATGACGA TGTCGGTGAG GCGGGAGCTG 
TGTGCTGTGA TGGTGTTCGC TGTGGTGGAA AGAGCTGGGA CCATCGTGTT AAACGACGGT 
GAAGAGCTGG ACTCATGGTC AGTGATCCTG AATGGTTCTG TGGAAGTGAC TTATCCAGAT 
GGAAAAGCAG AAATACTCTG TATGGGAAAT AGTTTCGGTG TCTCCCCCAC CATGGACAAG 
GAGTACATGA AAGGTGTGAT GAGGACAAAG GTGGATGACT GCCAGTTTGT CTGCATAGCC 
CAGCAAGATT ACTGCCGTAT CCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAAGAG 
GAAGGAGAGA TCGTCATGGT GAAGGAACAC CGAGAGCTTG ATCGGACTGG AACAAGAAAG 
GGGCACATTG TCATCAAGGG CACCTCAGAA AGGTTAACGA TGCATTTGGT GGAAGAACAT 
TCAGTGGTGG ATCCAACGTT CATAGAAGAC TTCCTGTTGA CCTATAGGAC TTTTCTCTGT 
AGCCCGATGG AAGTGGGCAA AAAGTTGTTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACGC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAATC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ACTGATGACG 
CTAACGAAAC CAGCCCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGAGG CTCAGAGAAA 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGCTAGCA AAGCAACAGA AGCGGGCTTG 
AAACGAGGGG ATCAGATACT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTATCA 
AAAGCCATGG AAATTCTTAG AAATAACACA CATTTATCTA TCACTGTGAA AACCAATTTA 
TTTGTATTTA AAGAACTTCT AACAAGATTA TCAGAAGAGA AGAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTCGCAGTA 
GATGTAGAGC AAGTGATAGG ACTTGAAAAA GTAAATAAGA AAAGCAAAGC CAACACTGTT 
GGGGGAAGGA ACAAGCTCAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATCGG GATTGGTCAG TCTCAAGATG ACAGCATAGT AGGATTAAGG 
CAGACTAAGC ACATCCCAAC TGCACTGCCT GTCAGCGGAA CCTTATCGTC CAGTAATCCT 
GACTTACTGC AGTCACACCA TCGCATTTTA GACTTCAGTG CTACTCCTGA TTTGCCAGAT 
CAAGTGCTAA GGGTTTTCAA GGCTGACCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACTACGGCGA AGGAAGTGGT CGTTCAGGCC ATCCGGGAGT TTGCCGTGAC CGCCACCCCA 
GATCAGTACT CGCTCTGTGA AGTCTCTGTC ACACCAGAGG GAGTGATCAA GCAAAGAAGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAC AGAATACAGC TGAGCGGAAG GTATTACTTG 
AAAAACAACA TGGAGACGGA AACCCTTTGC TCGGATGAGG ATGCACAGGA GCTGCTGCGA 
GAGAGCCAGA TCTCACTGCT GCAGCTCAGC ACCGTGGAGG TGGCCACGCA GCTCTCCATG 
CGCAACTTCG AGCTTTTCCG CAACATCGAG CCCACTGAGT ATATCGATGA CTTATTCAAG 
CTTAAATCAA AAACCAGCTG TGCCAACCTG AAGACATTCG AGGAAGTCAT TAACCAGGAG 
ACGTTTTGGG TAGCATCTGA GATTCTGCGG GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT AGCTCTGCAC TGTAGGGAGT GCAAGAATTT TAACTCCATG 
TTTGCGATCA TCAGTGGCCT AAACCTGGCG CCGGTGGCAA GACTGCGAAC TACCTGGGAG 
AAACTTCCCA ATAAATACGA AAAGCTGTTT CAAGATCTCC AAGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATACCG CAATGTCCTC AACAGTCAGA ACCTGCAGCC TCCTATCATC 
CCCCTGTTCC CAGTGATCAA GAAGGATCTT ACGTTCCTTC ACGAAGGGAA TGACTCCAAG 
GTTGATGGGC TGGTCAATTT TGAGAAGCTA AGAATGATTG CAAAGGAAAT ACGTCACGTT 
GGCCGAATGG CCTCTGTGAA CATGGACCCT GCCCTCATGT TCAGGACTCG GAAGAAGAAG 
TGGAGGAGTC TGGGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGTGGCAGT 
GGCAGTCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGATT TAACCAGCTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGGAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACTTTGGCTC CGAGTGGCAC CGTGGATAAC TTCTCAGATT CCGGCCACAG TGAAATCTCC 
TCACGATCCA GTATCGTCAG CAACTCCTCC TTCGATTCAT TGCCCGTCTC GCTGCCTGAC 
GAGCGGCGCC AGAGGCCGTC AGTCAGCATC GTGGAGACCA GCCTGGCCTC GGGCCGGCTG 
GAGAGGCGGC CGGCGGTGGA GCCTGACCAG TATAGCCTGG GTTCCTGTGC ACCACTGTCT 
GAGAGCAGGG GCCTATATGC TGCAGCCACC GTGATCTCTT CCCCCAGCAC GGAGGAGCTT 
TCCCAGGACC AGGGGGACCG AGCCTCCCTG GACGCTGCTG ACAGTGGCCG GGGGAGCTGG 
ACGTCGTGCT CGAGCGGCTC CCATGATAAC ATCCAGACCA TCCAGCACCA GAGGAGCTGG 
GAGACGCTCC CCTTTGGGCA CACTCACTTT GATTACCCAG GGGATGCTGC AGGGCTGTGG 
GCCTCGAGCA GCCATATGGA CCAAATCATG TTTCCCGACC ACAGTGCAAA GTACAGCAGG 
CAGAGTCAGA GCCGAGAGAG TCTCGATCAG GCCCAGTCCC GGGCCAGCTG GGCCTCGTCC 
ACAGGGTACT GGGGAGAGGA CTCGGAAGGG GACACGGGCA CCATCAAGAG AAGGGGGGGT 
AAGGACGTTT CCCTTGACGC TGACAGCAGC AGCATGACAG CCGTGACCGC AGAGGAAGCC 
AAACCTGCCG CCATGGCTGC CCACATAGCC GTGACACCCA GTGCTGCCAA GGGGCTCATC 
GCAGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832746.1
CDS	77..5065
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976978.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976978.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATTT TGGCAAGCGT TCTGCAGGAA GCTTTAGGCG TGGCTGTGAA 
TGCATTGTGT TAGAGCCTTC TGAAATGATT GTGGTAGACT ATATGGATGA AAATGAAGAA 
TATTTTCAGC GTCAAGCTTC TCATCGACAG TCTCGAAGGA GATTTAGAAA AATCAACCAG 
AAAGGGGAAA GACAAACTAT TATTGACACT GTGGATCCTT ATCCTGTGGG CAAACCACCT 
TTGCCTAGAG GCTATCATAC GGAATGCACT AAATCTCAGC TTCCTGCAGA TTTCACAAAG 
CTACACCTCA CTGACAGTCT CCACCCACAG GTGACCCACG TCTCCTCCAG CCACTCAGGA 
TGTAGCATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAG 
AGTGAGGCTG GTGATATGGA CCTTAGTGGG TTGCCAGAAA CAGCAGTGGA CTCTGAGGAC 
GATGATGATG AAGAGGACAT CGAGAGGGCC TCAGACCCTC TAATGAGCAG AGACATCGTG 
AGAGACTGCC TGGAGAAGGA CCCCATTGAT AGGACAGACG ATGACATTGA ACAACTTCTG 
GAATTCATGC ACCAGTTGCC TGCTTTTGCC AACATGACGA TGTCGGTGAG GCGGGAGCTG 
TGTGCTGTGA TGGTGTTCGC TGTGGTGGAA AGAGCTGGGA CCATCGTGTT AAACGACGGT 
GAAGAGCTGG ACTCATGGTC AGTGATCCTG AATGGTTCTG TGGAAGTGAC TTATCCAGAT 
GGAAAAGCAG AAATACTCTG TATGGGAAAT AGTTTCGGTG TCTCCCCCAC CATGGACAAG 
GAGTACATGA AAGGTGTGAT GAGGACAAAG GTGGATGACT GCCAGTTTGT CTGCATAGCC 
CAGCAAGATT ACTGCCGTAT CCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAAGAG 
GAAGGAGAGA TCGTCATGGT GAAGGAACAC CGAGAGCTTG ATCGGACTGG AACAAGAAAG 
GGGCACATTG TCATCAAGGG CACCTCAGAA AGGTTAACGA TGCATTTGGT GGAAGAACAT 
TCAGTGGTGG ATCCAACGTT CATAGAAGAC TTCCTGTTGA CCTATAGGAC TTTTCTCTGT 
AGCCCGATGG AAGTGGGCAA AAAGTTGTTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACGC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAATC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ACTGATGACG 
CTAACGAAAC CAGCCCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGAGG CTCAGAGAAA 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGCTAGCA AAGCAACAGA AGCGGGCTTG 
AAACGAGGGG ATCAGATACT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTATCA 
AAAGCCATGG AAATTCTTAG AAATAACACA CATTTATCTA TCACTGTGAA AACCAATTTA 
TTTGTATTTA AAGAACTTCT AACAAGATTA TCAGAAGAGA AGAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTCGCAGTA 
GATGTAGAGC AAGTGATAGG ACTTGAAAAA GTAAATAAGA AAAGCAAAGC CAACACTGTT 
GGGGGAAGGA ACAAGCTCAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATCGG GATTGGTCAG TCTCAAGATG ACAGCATAGT AGGATTAAGG 
CAGACTAAGC ACATCCCAAC TGCACTGCCT GTCAGCGGAA CCTTATCGTC CAGTAATCCT 
GACTTACTGC AGTCACACCA TCGCATTTTA GACTTCAGTG CTACTCCTGA TTTGCCAGAT 
CAAGTGCTAA GGGTTTTCAA GGCTGACCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACTACGGCGA AGGAAGTGGT CGTTCAGGCC ATCCGGGAGT TTGCCGTGAC CGCCACCCCA 
GATCAGTACT CGCTCTGTGA AGTCTCTGTC ACACCAGAGG GAGTGATCAA GCAAAGAAGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAC AGAATACAGC TGAGCGGAAG GTATTACTTG 
AAAAACAACA TGGAGACGGA AACCCTTTGC TCGGATGAGG ATGCACAGGA GCTGCTGCGA 
GAGAGCCAGA TCTCACTGCT GCAGCTCAGC ACCGTGGAGG TGGCCACGCA GCTCTCCATG 
CGCAACTTCG AGCTTTTCCG CAACATCGAG CCCACTGAGT ATATCGATGA CTTATTCAAG 
CTTAAATCAA AAACCAGCTG TGCCAACCTG AAGACATTCG AGGAAGTCAT TAACCAGGAG 
ACGTTTTGGG TAGCATCTGA GATTCTGCGG GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT AGCTCTGCAC TGTAGGGAGT GCAAGAATTT TAACTCCATG 
TTTGCGATCA TCAGTGGCCT AAACCTGGCG CCGGTGGCAA GACTGCGAAC TACCTGGGAG 
AAACTTCCCA ATAAATACGA AAAGCTGTTT CAAGATCTCC AAGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATACCG CAATGTCCTC AACAGTCAGA ACCTGCAGCC TCCTATCATC 
CCCCTGTTCC CAGTGATCAA GAAGGATCTT ACGTTCCTTC ACGAAGGGAA TGACTCCAAG 
GTTGATGGGC TGGTCAATTT TGAGAAGCTA AGAATGATTG CAAAGGAAAT ACGTCACGTT 
GGCCGAATGG CCTCTGTGAA CATGGACCCT GCCCTCATGT TCAGGACTCG GAAGAAGAAG 
TGGAGGAGTC TGGGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGTGGCAGT 
GGCAGTCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGATT TAACCAGCTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGGAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACTTTGGCTC CGAGTGGCAC CGTGGATAAC TTCTCAGATT CCGGCCACAG TGAAATCTCC 
TCACGATCCA GTATCGTCAG CAACTCCTCC TTCGATTCAT TGCCCGTCTC GCTGCCTGAC 
GAGCGGCGCC AGAGGCCGTC AGTCAGCATC GTGGAGACCA GCCTGGCCTC GGGCCGGCTG 
GAGAGGCGGC CGGCGGTGGA GCCTGACCAG TATAGCCTGG GTTCCTGTGC ACCACTGTCT 
GAGAGCAGGG GCCTATATGC TGCAGCCACC GTGATCTCTT CCCCCAGCAC GGAGGAGCTT 
TCCCAGGACC AGGGGGACCG AGCCTCCCTG GACGCTGCTG ACAGTGGCCG GGGGAGCTGG 
ACGTCGTGCT CGAGCGGCTC CCATGATAAC ATCCAGACCA TCCAGCACCA GAGGAGCTGG 
GAGACGCTCC CCTTTGGGCA CACTCACTTT GATTACCCAG GGGATGCTGC AGGGCTGTGG 
GCCTCGAGCA GCCATATGGA CCAAATCATG TTTCCCGACC ACAGTGCAAA GTACAGCAGG 
CAGAGTCAGA GCCGAGAGAG TCTCGATCAG GCCCAGTCCC GGGCCAGCTG GGCCTCGTCC 
ACAGGGTACT GGGGAGAGGA CTCGGAAGGG GACACGGGCA CCATCAAGAG AAGGGGGGGT 
AAGGACGTTT CCCTTGACGC TGACAGCAGC AGCATGACAG CCGTGACCGC AGAGGAAGCC 
AAACCTGCCG CCATGGCTGC CCACATAGCC GTGACACCCA GTGCTGCCAA GGGGCTCATC 
GCAGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976979.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832747.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

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CloneID	OBa224126
Clone ID Related Accession (Same CDS sequence)	XM_024976979.1
Accession Version	XM_024976979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832747.1
CDS	77..5026
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976979.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976979.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976981.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832749.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa224127
Clone ID Related Accession (Same CDS sequence)	XM_024976981.1
Accession Version	XM_024976981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832749.1
CDS	53..4999
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976981.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976981.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976982.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832750.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa224128
Clone ID Related Accession (Same CDS sequence)	XM_024976982.1
Accession Version	XM_024976982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832750.1
CDS	77..5023
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976982.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976982.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976983.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832751.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.

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CloneID	OBa224129
Clone ID Related Accession (Same CDS sequence)	XM_024976983.1
Accession Version	XM_024976983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGAA 
GATGAACAAG TGTCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832751.1
CDS	77..5020
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976983.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976983.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGAA 
GATGAACAAG TGTCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976984.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832752.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X15, mRNA.

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CloneID	OBa224130
Clone ID Related Accession (Same CDS sequence)	XM_024976984.1
Accession Version	XM_024976984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGCTTCC TGCAGATTTC 
ACAAAGCTAC ACCTCACTGA CAGTCTCCAC CCACAGGTGA CCCACGTCTC CTCCAGCCAC 
TCAGGATGTA GCATCACTAG TGATTCTGGA AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAGAGTG AGGCTGGTGA TATGGACCTT AGTGGGTTGC CAGAAACAGC AGTGGACTCT 
GAGGACGATG ATGATGAAGA GGACATCGAG AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC 
ATCGTGAGAG ACTGCCTGGA GAAGGACCCC ATTGATAGGA CAGACGATGA CATTGAACAA 
CTTCTGGAAT TCATGCACCA GTTGCCTGCT TTTGCCAACA TGACGATGTC GGTGAGGCGG 
GAGCTGTGTG CTGTGATGGT GTTCGCTGTG GTGGAAAGAG CTGGGACCAT CGTGTTAAAC 
GACGGTGAAG AGCTGGACTC ATGGTCAGTG ATCCTGAATG GTTCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTCTGTATG GGAAATAGTT TCGGTGTCTC CCCCACCATG 
GACAAGGAGT ACATGAAAGG TGTGATGAGG ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATCCTC AACCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATCGT CATGGTGAAG GAACACCGAG AGCTTGATCG GACTGGAACA 
AGAAAGGGGC ACATTGTCAT CAAGGGCACC TCAGAAAGGT TAACGATGCA TTTGGTGGAA 
GAACATTCAG TGGTGGATCC AACGTTCATA GAAGACTTCC TGTTGACCTA TAGGACTTTT 
CTCTGTAGCC CGATGGAAGT GGGCAAAAAG TTGTTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACGCGGGT AGTATTATTG TGGGTGAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTCTAGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ATCTAAGGCT GTTAAATATT GCATGTGCAG CTAAAGCAAA AAGAAGACTG 
ATGACGCTAA CGAAACCAGC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCA 
GAGAAAGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG CTAGCAAAGC AACAGAAGCG 
GGCTTGAAAC GAGGGGATCA GATACTAGAA GTAAATGGTC AAAACTTTGA AAATATTCAG 
CTATCAAAAG CCATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTATCAG AAGAGAAGAG AAATGGTGCC 
CCTCACCTCC CTAAAATTGG AGACATCAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTC 
GCAGTAGATG TAGAGCAAGT GATAGGACTT GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC 
ACTGTTGGGG GAAGGAACAA GCTCAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATATAATGA TATCGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CTAAGCACAT CCCAACTGCA CTGCCTGTCA GCGGAACCTT ATCGTCCAGT 
AATCCTGACT TACTGCAGTC ACACCATCGC ATTTTAGACT TCAGTGCTAC TCCTGATTTG 
CCAGATCAAG TGCTAAGGGT TTTCAAGGCT GACCAGCAAA GCCGATACAT CATGATCAGT 
AAGGACACTA CGGCGAAGGA AGTGGTCGTT CAGGCCATCC GGGAGTTTGC CGTGACCGCC 
ACCCCAGATC AGTACTCGCT CTGTGAAGTC TCTGTCACAC CAGAGGGAGT GATCAAGCAA 
AGAAGGCTTC CAGATCAGCT TTCCAAACTT GCTGACAGAA TACAGCTGAG CGGAAGGTAT 
TACTTGAAAA ACAACATGGA GACGGAAACC CTTTGCTCGG ATGAGGATGC ACAGGAGCTG 
CTGCGAGAGA GCCAGATCTC ACTGCTGCAG CTCAGCACCG TGGAGGTGGC CACGCAGCTC 
TCCATGCGCA ACTTCGAGCT TTTCCGCAAC ATCGAGCCCA CTGAGTATAT CGATGACTTA 
TTCAAGCTTA AATCAAAAAC CAGCTGTGCC AACCTGAAGA CATTCGAGGA AGTCATTAAC 
CAGGAGACGT TTTGGGTAGC ATCTGAGATT CTGCGGGAGA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCT CTGCACTGTA GGGAGTGCAA GAATTTTAAC 
TCCATGTTTG CGATCATCAG TGGCCTAAAC CTGGCGCCGG TGGCAAGACT GCGAACTACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAG CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATACCGCAAT GTCCTCAACA GTCAGAACCT GCAGCCTCCT 
ATCATCCCCC TGTTCCCAGT GATCAAGAAG GATCTTACGT TCCTTCACGA AGGGAATGAC 
TCCAAGGTTG ATGGGCTGGT CAATTTTGAG AAGCTAAGAA TGATTGCAAA GGAAATACGT 
CACGTTGGCC GAATGGCCTC TGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC CAGGGTAGTA CAAACGCAAC CGTGCTAGAT 
GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG GTGCGTCGTA GCTCCTTTCT CAATGCCAAA 
AAGCTATATG AAGATGCCCA GATGGCTCGC AAAGTGAAGC AGTACCTGTC CAATCTGGAG 
CTCGAGATGG ATGAGGAGAG TCTTCAGACG CTATCCCTGC AGTGTGAGCC GGCCACCAAC 
ACACTGCCTA AGAATCCCAC TGACAAAAAG CCTGTCAAAT CGGAGACCTC CCCAGTGGCC 
CCGAGAGCGG GGTTGCAGCC GAAAGCACAG CCGCAGCCCC AGCCGCCGCA GCCGCCACAC 
AAACTCAACC AGGGACTGCA GGTTCCCGCC GTGTCCCTTT ACCCTTCACG GAAGAAAGTT 
CCCGTAAAGG ACCTCCCACC TTTCGGCATC AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC CATAGGAGGC CAGCAGAAGA CACGATATCC 
AACACGTCTT CCCAGCTCTC CTCTCCGCCC ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC 
TACACTTTGG CTCCGAGTGG CACCGTGGAT AACTTCTCAG ATTCCGGCCA CAGTGAAATC 
TCCTCACGAT CCAGTATCGT CAGCAACTCC TCCTTCGATT CATTGCCCGT CTCGCTGCCT 
GACGAGCGGC GCCAGAGGCC GTCAGTCAGC ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG 
CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC CAGTATAGCC TGGGTTCCTG TGCACCACTG 
TCTGAGAGCA GGGGCCTATA TGCTGCAGCC ACCGTGATCT CTTCCCCCAG CACGGAGGAG 
CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC CTGGACGCTG CTGACAGTGG CCGGGGGAGC 
TGGACGTCGT GCTCGAGCGG CTCCCATGAT AACATCCAGA CCATCCAGCA CCAGAGGAGC 
TGGGAGACGC TCCCCTTTGG GCACACTCAC TTTGATTACC CAGGGGATGC TGCAGGGCTG 
TGGGCCTCGA GCAGCCATAT GGACCAAATC ATGTTTCCCG ACCACAGTGC AAAGTACAGC 
AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG 
TCCACAGGGT ACTGGGGAGA GGACTCGGAA GGGGACACGG GCACCATCAA GAGAAGGGGG 
GGTAAGGACG TTTCCCTTGA CGCTGACAGC AGCAGCATGA CAGCCGTGAC CGCAGAGGAA 
GCCAAACCTG CCGCCATGGC TGCCCACATA GCCGTGACAC CCAGTGCTGC CAAGGGGCTC 
ATCGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832752.1
CDS	77..5005
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976984.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976984.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGCTTCC TGCAGATTTC 
ACAAAGCTAC ACCTCACTGA CAGTCTCCAC CCACAGGTGA CCCACGTCTC CTCCAGCCAC 
TCAGGATGTA GCATCACTAG TGATTCTGGA AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAGAGTG AGGCTGGTGA TATGGACCTT AGTGGGTTGC CAGAAACAGC AGTGGACTCT 
GAGGACGATG ATGATGAAGA GGACATCGAG AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC 
ATCGTGAGAG ACTGCCTGGA GAAGGACCCC ATTGATAGGA CAGACGATGA CATTGAACAA 
CTTCTGGAAT TCATGCACCA GTTGCCTGCT TTTGCCAACA TGACGATGTC GGTGAGGCGG 
GAGCTGTGTG CTGTGATGGT GTTCGCTGTG GTGGAAAGAG CTGGGACCAT CGTGTTAAAC 
GACGGTGAAG AGCTGGACTC ATGGTCAGTG ATCCTGAATG GTTCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTCTGTATG GGAAATAGTT TCGGTGTCTC CCCCACCATG 
GACAAGGAGT ACATGAAAGG TGTGATGAGG ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATCCTC AACCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATCGT CATGGTGAAG GAACACCGAG AGCTTGATCG GACTGGAACA 
AGAAAGGGGC ACATTGTCAT CAAGGGCACC TCAGAAAGGT TAACGATGCA TTTGGTGGAA 
GAACATTCAG TGGTGGATCC AACGTTCATA GAAGACTTCC TGTTGACCTA TAGGACTTTT 
CTCTGTAGCC CGATGGAAGT GGGCAAAAAG TTGTTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACGCGGGT AGTATTATTG TGGGTGAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTCTAGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ATCTAAGGCT GTTAAATATT GCATGTGCAG CTAAAGCAAA AAGAAGACTG 
ATGACGCTAA CGAAACCAGC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCA 
GAGAAAGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG CTAGCAAAGC AACAGAAGCG 
GGCTTGAAAC GAGGGGATCA GATACTAGAA GTAAATGGTC AAAACTTTGA AAATATTCAG 
CTATCAAAAG CCATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTATCAG AAGAGAAGAG AAATGGTGCC 
CCTCACCTCC CTAAAATTGG AGACATCAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTC 
GCAGTAGATG TAGAGCAAGT GATAGGACTT GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC 
ACTGTTGGGG GAAGGAACAA GCTCAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATATAATGA TATCGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CTAAGCACAT CCCAACTGCA CTGCCTGTCA GCGGAACCTT ATCGTCCAGT 
AATCCTGACT TACTGCAGTC ACACCATCGC ATTTTAGACT TCAGTGCTAC TCCTGATTTG 
CCAGATCAAG TGCTAAGGGT TTTCAAGGCT GACCAGCAAA GCCGATACAT CATGATCAGT 
AAGGACACTA CGGCGAAGGA AGTGGTCGTT CAGGCCATCC GGGAGTTTGC CGTGACCGCC 
ACCCCAGATC AGTACTCGCT CTGTGAAGTC TCTGTCACAC CAGAGGGAGT GATCAAGCAA 
AGAAGGCTTC CAGATCAGCT TTCCAAACTT GCTGACAGAA TACAGCTGAG CGGAAGGTAT 
TACTTGAAAA ACAACATGGA GACGGAAACC CTTTGCTCGG ATGAGGATGC ACAGGAGCTG 
CTGCGAGAGA GCCAGATCTC ACTGCTGCAG CTCAGCACCG TGGAGGTGGC CACGCAGCTC 
TCCATGCGCA ACTTCGAGCT TTTCCGCAAC ATCGAGCCCA CTGAGTATAT CGATGACTTA 
TTCAAGCTTA AATCAAAAAC CAGCTGTGCC AACCTGAAGA CATTCGAGGA AGTCATTAAC 
CAGGAGACGT TTTGGGTAGC ATCTGAGATT CTGCGGGAGA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCT CTGCACTGTA GGGAGTGCAA GAATTTTAAC 
TCCATGTTTG CGATCATCAG TGGCCTAAAC CTGGCGCCGG TGGCAAGACT GCGAACTACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAG CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATACCGCAAT GTCCTCAACA GTCAGAACCT GCAGCCTCCT 
ATCATCCCCC TGTTCCCAGT GATCAAGAAG GATCTTACGT TCCTTCACGA AGGGAATGAC 
TCCAAGGTTG ATGGGCTGGT CAATTTTGAG AAGCTAAGAA TGATTGCAAA GGAAATACGT 
CACGTTGGCC GAATGGCCTC TGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC CAGGGTAGTA CAAACGCAAC CGTGCTAGAT 
GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG GTGCGTCGTA GCTCCTTTCT CAATGCCAAA 
AAGCTATATG AAGATGCCCA GATGGCTCGC AAAGTGAAGC AGTACCTGTC CAATCTGGAG 
CTCGAGATGG ATGAGGAGAG TCTTCAGACG CTATCCCTGC AGTGTGAGCC GGCCACCAAC 
ACACTGCCTA AGAATCCCAC TGACAAAAAG CCTGTCAAAT CGGAGACCTC CCCAGTGGCC 
CCGAGAGCGG GGTTGCAGCC GAAAGCACAG CCGCAGCCCC AGCCGCCGCA GCCGCCACAC 
AAACTCAACC AGGGACTGCA GGTTCCCGCC GTGTCCCTTT ACCCTTCACG GAAGAAAGTT 
CCCGTAAAGG ACCTCCCACC TTTCGGCATC AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC CATAGGAGGC CAGCAGAAGA CACGATATCC 
AACACGTCTT CCCAGCTCTC CTCTCCGCCC ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC 
TACACTTTGG CTCCGAGTGG CACCGTGGAT AACTTCTCAG ATTCCGGCCA CAGTGAAATC 
TCCTCACGAT CCAGTATCGT CAGCAACTCC TCCTTCGATT CATTGCCCGT CTCGCTGCCT 
GACGAGCGGC GCCAGAGGCC GTCAGTCAGC ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG 
CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC CAGTATAGCC TGGGTTCCTG TGCACCACTG 
TCTGAGAGCA GGGGCCTATA TGCTGCAGCC ACCGTGATCT CTTCCCCCAG CACGGAGGAG 
CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC CTGGACGCTG CTGACAGTGG CCGGGGGAGC 
TGGACGTCGT GCTCGAGCGG CTCCCATGAT AACATCCAGA CCATCCAGCA CCAGAGGAGC 
TGGGAGACGC TCCCCTTTGG GCACACTCAC TTTGATTACC CAGGGGATGC TGCAGGGCTG 
TGGGCCTCGA GCAGCCATAT GGACCAAATC ATGTTTCCCG ACCACAGTGC AAAGTACAGC 
AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG 
TCCACAGGGT ACTGGGGAGA GGACTCGGAA GGGGACACGG GCACCATCAA GAGAAGGGGG 
GGTAAGGACG TTTCCCTTGA CGCTGACAGC AGCAGCATGA CAGCCGTGAC CGCAGAGGAA 
GCCAAACCTG CCGCCATGGC TGCCCACATA GCCGTGACAC CCAGTGCTGC CAAGGGGCTC 
ATCGCCAGGA AGGAGGGCCG CTACCGGGAG CCGCCGCCCA CTCCACCAGG CTACGTGGGC 
ATCCCCATCA CTGACTTCCC CGAGGCCCAC CCGCACCCGG CCCGGAAACC GCCGGACTAC 
ACTGTGGCGC TGCAGCGGTC CAGGATGCTG GCCCGGCCGG CCGAACCCCC TGCCCCAGGC 
AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG CACCGGCCTG GTGATGGTGA CCCACGCGCC 
GGCCCCTGTG CACCCCCGGG CCTCACCGCC GAGGAGGACG CAGAAGATGA ACAAGTGTCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976985.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832753.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X16, mRNA.

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CloneID	OBa224131
Clone ID Related Accession (Same CDS sequence)	XM_024976985.1
Accession Version	XM_024976985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGCTACACT 
TTGGCTCCGA GTGGCACCGT GGATAACTTC TCAGATTCCG GCCACAGTGA AATCTCCTCA 
CGATCCAGTA TCGTCAGCAA CTCCTCCTTC GATTCATTGC CCGTCTCGCT GCCTGACGAG 
CGGCGCCAGA GGCCGTCAGT CAGCATCGTG GAGACCAGCC TGGCCTCGGG CCGGCTGGAG 
AGGCGGCCGG CGGTGGAGCC TGACCAGTAT AGCCTGGGTT CCTGTGCACC ACTGTCTGAG 
AGCAGGGGCC TATATGCTGC AGCCACCGTG ATCTCTTCCC CCAGCACGGA GGAGCTTTCC 
CAGGACCAGG GGGACCGAGC CTCCCTGGAC GCTGCTGACA GTGGCCGGGG GAGCTGGACG 
TCGTGCTCGA GCGGCTCCCA TGATAACATC CAGACCATCC AGCACCAGAG GAGCTGGGAG 
ACGCTCCCCT TTGGGCACAC TCACTTTGAT TACCCAGGGG ATGCTGCAGG GCTGTGGGCC 
TCGAGCAGCC ATATGGACCA AATCATGTTT CCCGACCACA GTGCAAAGTA CAGCAGGCAG 
AGTCAGAGCC GAGAGAGTCT CGATCAGGCC CAGTCCCGGG CCAGCTGGGC CTCGTCCACA 
GGGTACTGGG GAGAGGACTC GGAAGGGGAC ACGGGCACCA TCAAGAGAAG GGGGGGTAAG 
GACGTTTCCC TTGACGCTGA CAGCAGCAGC ATGACAGCCG TGACCGCAGA GGAAGCCAAA 
CCTGCCGCCA TGGCTGCCCA CATAGCCGTG ACACCCAGTG CTGCCAAGGG GCTCATCGCA 
GCCAGGAAGG AGGGCCGCTA CCGGGAGCCG CCGCCCACTC CACCAGGCTA CGTGGGCATC 
CCCATCACTG ACTTCCCCGA GGCCCACCCG CACCCGGCCC GGAAACCGCC GGACTACACT 
GTGGCGCTGC AGCGGTCCAG GATGCTGGCC CGGCCGGCCG AACCCCCTGC CCCAGGCAGC 
GCCAGGCCTG CCCCCAGGCC GCAGTGGCAC CGGCCTGGTG ATGGTGACCC ACGCGCCGGC 
CCCTGTGCAC CCCCGGGCCT CACCGCCGAG GAGGACGCAG AAGATGAACA AGTGTCTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832753.1
CDS	77..5002
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976985.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976985.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGCTACACT 
TTGGCTCCGA GTGGCACCGT GGATAACTTC TCAGATTCCG GCCACAGTGA AATCTCCTCA 
CGATCCAGTA TCGTCAGCAA CTCCTCCTTC GATTCATTGC CCGTCTCGCT GCCTGACGAG 
CGGCGCCAGA GGCCGTCAGT CAGCATCGTG GAGACCAGCC TGGCCTCGGG CCGGCTGGAG 
AGGCGGCCGG CGGTGGAGCC TGACCAGTAT AGCCTGGGTT CCTGTGCACC ACTGTCTGAG 
AGCAGGGGCC TATATGCTGC AGCCACCGTG ATCTCTTCCC CCAGCACGGA GGAGCTTTCC 
CAGGACCAGG GGGACCGAGC CTCCCTGGAC GCTGCTGACA GTGGCCGGGG GAGCTGGACG 
TCGTGCTCGA GCGGCTCCCA TGATAACATC CAGACCATCC AGCACCAGAG GAGCTGGGAG 
ACGCTCCCCT TTGGGCACAC TCACTTTGAT TACCCAGGGG ATGCTGCAGG GCTGTGGGCC 
TCGAGCAGCC ATATGGACCA AATCATGTTT CCCGACCACA GTGCAAAGTA CAGCAGGCAG 
AGTCAGAGCC GAGAGAGTCT CGATCAGGCC CAGTCCCGGG CCAGCTGGGC CTCGTCCACA 
GGGTACTGGG GAGAGGACTC GGAAGGGGAC ACGGGCACCA TCAAGAGAAG GGGGGGTAAG 
GACGTTTCCC TTGACGCTGA CAGCAGCAGC ATGACAGCCG TGACCGCAGA GGAAGCCAAA 
CCTGCCGCCA TGGCTGCCCA CATAGCCGTG ACACCCAGTG CTGCCAAGGG GCTCATCGCA 
GCCAGGAAGG AGGGCCGCTA CCGGGAGCCG CCGCCCACTC CACCAGGCTA CGTGGGCATC 
CCCATCACTG ACTTCCCCGA GGCCCACCCG CACCCGGCCC GGAAACCGCC GGACTACACT 
GTGGCGCTGC AGCGGTCCAG GATGCTGGCC CGGCCGGCCG AACCCCCTGC CCCAGGCAGC 
GCCAGGCCTG CCCCCAGGCC GCAGTGGCAC CGGCCTGGTG ATGGTGACCC ACGCGCCGGC 
CCCTGTGCAC CCCCGGGCCT CACCGCCGAG GAGGACGCAG AAGATGAACA AGTGTCTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976986.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832754.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa224132
Clone ID Related Accession (Same CDS sequence)	XM_024976986.1
Accession Version	XM_024976986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGCTACACT 
TTGGCTCCGA GTGGCACCGT GGATAACTTC TCAGATTCCG GCCACAGTGA AATCTCCTCA 
CGATCCAGTA TCGTCAGCAA CTCCTCCTTC GATTCATTGC CCGTCTCGCT GCCTGACGAG 
CGGCGCCAGA GGCCGTCAGT CAGCATCGTG GAGACCAGCC TGGCCTCGGG CCGGCTGGAG 
AGGCGGCCGG CGGTGGAGCC TGACCAGTAT AGCCTGGGTT CCTGTGCACC ACTGTCTGAG 
AGCAGGGGCC TATATGCTGC AGCCACCGTG ATCTCTTCCC CCAGCACGGA GGAGCTTTCC 
CAGGACCAGG GGGACCGAGC CTCCCTGGAC GCTGCTGACA GTGGCCGGGG GAGCTGGACG 
TCGTGCTCGA GCGGCTCCCA TGATAACATC CAGACCATCC AGCACCAGAG GAGCTGGGAG 
ACGCTCCCCT TTGGGCACAC TCACTTTGAT TACCCAGGGG ATGCTGCAGG GCTGTGGGCC 
TCGAGCAGCC ATATGGACCA AATCATGTTT CCCGACCACA GTGCAAAGTA CAGCAGGCAG 
AGTCAGAGCC GAGAGAGTCT CGATCAGGCC CAGTCCCGGG CCAGCTGGGC CTCGTCCACA 
GGGTACTGGG GAGAGGACTC GGAAGGGGAC ACGGGCACCA TCAAGAGAAG GGGGGGTAAG 
GACGTTTCCC TTGACGCTGA CAGCAGCAGC ATGACAGCCG TGACCGCAGA GGAAGCCAAA 
CCTGCCGCCA TGGCTGCCCA CATAGCCGTG ACACCCAGTG CTGCCAAGGG GCTCATCGCA 
GCCAGGAAGG AGGGCCGCTA CCGGGAGCCG CCGCCCACTC CACCAGGCTA CGTGGGCATC 
CCCATCACTG ACTTCCCCGA GGCCCACCCG CACCCGGCCC GGAAACCGCC GGACTACACT 
GTGGCGCTGC AGCGGTCCAG GATGCTGGCC CGGCCGGCCG AACCCCCTGC CCCAGGCAGC 
GCCAGGCCTG CCCCCAGGCC GCAGTGGCAC CGGCCTGGTG ATGGTGACCC ACGCGCCGGC 
CCCTGTGCAC CCCCGGGCCT CACCGCCGAG GAGGACGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832754.1
CDS	53..4975
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976986.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976986.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGG CATCAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGCCTGGA GCGCCATAGG AGGCCAGCAG AAGACACGAT ATCCAACACG 
TCTTCCCAGC TCTCCTCTCC GCCCACCTCC CCACAGAGCT CCCCACGGAA AGGCTACACT 
TTGGCTCCGA GTGGCACCGT GGATAACTTC TCAGATTCCG GCCACAGTGA AATCTCCTCA 
CGATCCAGTA TCGTCAGCAA CTCCTCCTTC GATTCATTGC CCGTCTCGCT GCCTGACGAG 
CGGCGCCAGA GGCCGTCAGT CAGCATCGTG GAGACCAGCC TGGCCTCGGG CCGGCTGGAG 
AGGCGGCCGG CGGTGGAGCC TGACCAGTAT AGCCTGGGTT CCTGTGCACC ACTGTCTGAG 
AGCAGGGGCC TATATGCTGC AGCCACCGTG ATCTCTTCCC CCAGCACGGA GGAGCTTTCC 
CAGGACCAGG GGGACCGAGC CTCCCTGGAC GCTGCTGACA GTGGCCGGGG GAGCTGGACG 
TCGTGCTCGA GCGGCTCCCA TGATAACATC CAGACCATCC AGCACCAGAG GAGCTGGGAG 
ACGCTCCCCT TTGGGCACAC TCACTTTGAT TACCCAGGGG ATGCTGCAGG GCTGTGGGCC 
TCGAGCAGCC ATATGGACCA AATCATGTTT CCCGACCACA GTGCAAAGTA CAGCAGGCAG 
AGTCAGAGCC GAGAGAGTCT CGATCAGGCC CAGTCCCGGG CCAGCTGGGC CTCGTCCACA 
GGGTACTGGG GAGAGGACTC GGAAGGGGAC ACGGGCACCA TCAAGAGAAG GGGGGGTAAG 
GACGTTTCCC TTGACGCTGA CAGCAGCAGC ATGACAGCCG TGACCGCAGA GGAAGCCAAA 
CCTGCCGCCA TGGCTGCCCA CATAGCCGTG ACACCCAGTG CTGCCAAGGG GCTCATCGCA 
GCCAGGAAGG AGGGCCGCTA CCGGGAGCCG CCGCCCACTC CACCAGGCTA CGTGGGCATC 
CCCATCACTG ACTTCCCCGA GGCCCACCCG CACCCGGCCC GGAAACCGCC GGACTACACT 
GTGGCGCTGC AGCGGTCCAG GATGCTGGCC CGGCCGGCCG AACCCCCTGC CCCAGGCAGC 
GCCAGGCCTG CCCCCAGGCC GCAGTGGCAC CGGCCTGGTG ATGGTGACCC ACGCGCCGGC 
CCCTGTGCAC CCCCGGGCCT CACCGCCGAG GAGGACGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976987.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832755.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa224133
Clone ID Related Accession (Same CDS sequence)	XM_024976987.1
Accession Version	XM_024976987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCAGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832755.1
CDS	77..4978
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976987.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976987.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCAGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976988.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832756.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X19, mRNA.

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CloneID	OBa224134
Clone ID Related Accession (Same CDS sequence)	XM_024976988.1
Accession Version	XM_024976988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGA AGGAAGCCTG GAGCGCCATA GGAGGCCAGC AGAAGACACG 
ATATCCAACA CGTCTTCCCA GCTCTCCTCT CCGCCCACCT CCCCACAGAG CTCCCCACGG 
AAAGGCTACA CTTTGGCTCC GAGTGGCACC GTGGATAACT TCTCAGATTC CGGCCACAGT 
GAAATCTCCT CACGATCCAG TATCGTCAGC AACTCCTCCT TCGATTCATT GCCCGTCTCG 
CTGCCTGACG AGCGGCGCCA GAGGCCGTCA GTCAGCATCG TGGAGACCAG CCTGGCCTCG 
GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG CCTGACCAGT ATAGCCTGGG TTCCTGTGCA 
CCACTGTCTG AGAGCAGGGG CCTATATGCT GCAGCCACCG TGATCTCTTC CCCCAGCACG 
GAGGAGCTTT CCCAGGACCA GGGGGACCGA GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG 
GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC CATGATAACA TCCAGACCAT CCAGCACCAG 
AGGAGCTGGG AGACGCTCCC CTTTGGGCAC ACTCACTTTG ATTACCCAGG GGATGCTGCA 
GGGCTGTGGG CCTCGAGCAG CCATATGGAC CAAATCATGT TTCCCGACCA CAGTGCAAAG 
TACAGCAGGC AGAGTCAGAG CCGAGAGAGT CTCGATCAGG CCCAGTCCCG GGCCAGCTGG 
GCCTCGTCCA CAGGGTACTG GGGAGAGGAC TCGGAAGGGG ACACGGGCAC CATCAAGAGA 
AGGGGGGGTA AGGACGTTTC CCTTGACGCT GACAGCAGCA GCATGACAGC CGTGACCGCA 
GAGGAAGCCA AACCTGCCGC CATGGCTGCC CACATAGCCG TGACACCCAG TGCTGCCAAG 
GGGCTCATCG CCAGGAAGGA GGGCCGCTAC CGGGAGCCGC CGCCCACTCC ACCAGGCTAC 
GTGGGCATCC CCATCACTGA CTTCCCCGAG GCCCACCCGC ACCCGGCCCG GAAACCGCCG 
GACTACACTG TGGCGCTGCA GCGGTCCAGG ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC 
CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG CAGTGGCACC GGCCTGGTGA TGGTGACCCA 
CGCGCCGGCC CCTGTGCACC CCCGGGCCTC ACCGCCGAGG AGGACGAAGA TGAACAAGTG 
TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832756.1
CDS	77..4948
Translation	MASYVDNSFRQAVMKNPAERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976988.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976988.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCCAAC 
CTGAGAGAGC ATCAGCTTAG GTTGATGTGT GAAACTGTGA GATACGAAAG ACATGAAGCC 
AATGAGGTTT TGTACTATCC TGATGATATT GGGACCTGCT GGTACATCCT TCTCTCCGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCACGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTGTTAGAGC CTTCTGAAAT GATTGTGGTA 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCATCG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGG GAAAGACAAA CTATTATTGA CACTGTGGAT 
CCTTATCCTG TGGGCAAACC ACCTTTGCCT AGAGGCTATC ATACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCGCT CAGCCAGGGT AGTACAAACG CAACCGTGCT AGATGTTGCT 
CAGACAGGTG GGCATAAAAA GCGGGTGCGT CGTAGCTCCT TTCTCAATGC CAAAAAGCTA 
TATGAAGATG CCCAGATGGC TCGCAAAGTG AAGCAGTACC TGTCCAATCT GGAGCTCGAG 
ATGGATGAGG AGAGTCTTCA GACGCTATCC CTGCAGTGTG AGCCGGCCAC CAACACACTG 
CCTAAGAATC CCACTGACAA AAAGCCTGTC AAATCGGAGA CCTCCCCAGT GGCCCCGAGA 
GCGGGGTTGC AGCCGAAAGC ACAGCCGCAG CCCCAGCCGC CGCAGCCGCC ACACAAACTC 
AACCAGGGAC TGCAGGTTCC CGCCGTGTCC CTTTACCCTT CACGGAAGAA AGTTCCCGTA 
AAGGACCTCC CACCTTTCGA AGGAAGCCTG GAGCGCCATA GGAGGCCAGC AGAAGACACG 
ATATCCAACA CGTCTTCCCA GCTCTCCTCT CCGCCCACCT CCCCACAGAG CTCCCCACGG 
AAAGGCTACA CTTTGGCTCC GAGTGGCACC GTGGATAACT TCTCAGATTC CGGCCACAGT 
GAAATCTCCT CACGATCCAG TATCGTCAGC AACTCCTCCT TCGATTCATT GCCCGTCTCG 
CTGCCTGACG AGCGGCGCCA GAGGCCGTCA GTCAGCATCG TGGAGACCAG CCTGGCCTCG 
GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG CCTGACCAGT ATAGCCTGGG TTCCTGTGCA 
CCACTGTCTG AGAGCAGGGG CCTATATGCT GCAGCCACCG TGATCTCTTC CCCCAGCACG 
GAGGAGCTTT CCCAGGACCA GGGGGACCGA GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG 
GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC CATGATAACA TCCAGACCAT CCAGCACCAG 
AGGAGCTGGG AGACGCTCCC CTTTGGGCAC ACTCACTTTG ATTACCCAGG GGATGCTGCA 
GGGCTGTGGG CCTCGAGCAG CCATATGGAC CAAATCATGT TTCCCGACCA CAGTGCAAAG 
TACAGCAGGC AGAGTCAGAG CCGAGAGAGT CTCGATCAGG CCCAGTCCCG GGCCAGCTGG 
GCCTCGTCCA CAGGGTACTG GGGAGAGGAC TCGGAAGGGG ACACGGGCAC CATCAAGAGA 
AGGGGGGGTA AGGACGTTTC CCTTGACGCT GACAGCAGCA GCATGACAGC CGTGACCGCA 
GAGGAAGCCA AACCTGCCGC CATGGCTGCC CACATAGCCG TGACACCCAG TGCTGCCAAG 
GGGCTCATCG CCAGGAAGGA GGGCCGCTAC CGGGAGCCGC CGCCCACTCC ACCAGGCTAC 
GTGGGCATCC CCATCACTGA CTTCCCCGAG GCCCACCCGC ACCCGGCCCG GAAACCGCCG 
GACTACACTG TGGCGCTGCA GCGGTCCAGG ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC 
CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG CAGTGGCACC GGCCTGGTGA TGGTGACCCA 
CGCGCCGGCC CCTGTGCACC CCCGGGCCTC ACCGCCGAGG AGGACGAAGA TGAACAAGTG 
TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976989.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832757.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X21, mRNA.

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CloneID	OBa224135
Clone ID Related Accession (Same CDS sequence)	XM_024976989.1
Accession Version	XM_024976989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAT GTGGTCTACC CGTAGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGT 
CAAGCTTCTC ATCGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGGGAAAGA 
CAAACTATTA TTGACACTGT GGATCCTTAT CCTGTGGGCA AACCACCTTT GCCTAGAGGC 
TATCATACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAGCT ACACCTCACT 
GACAGTCTCC ACCCACAGGT GACCCACGTC TCCTCCAGCC ACTCAGGATG TAGCATCACT 
AGTGATTCTG GAAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAGAG TGAGGCTGGT 
GATATGGACC TTAGTGGGTT GCCAGAAACA GCAGTGGACT CTGAGGACGA TGATGATGAA 
GAGGACATCG AGAGGGCCTC AGACCCTCTA ATGAGCAGAG ACATCGTGAG AGACTGCCTG 
GAGAAGGACC CCATTGATAG GACAGACGAT GACATTGAAC AACTTCTGGA ATTCATGCAC 
CAGTTGCCTG CTTTTGCCAA CATGACGATG TCGGTGAGGC GGGAGCTGTG TGCTGTGATG 
GTGTTCGCTG TGGTGGAAAG AGCTGGGACC ATCGTGTTAA ACGACGGTGA AGAGCTGGAC 
TCATGGTCAG TGATCCTGAA TGGTTCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA 
ATACTCTGTA TGGGAAATAG TTTCGGTGTC TCCCCCACCA TGGACAAGGA GTACATGAAA 
GGTGTGATGA GGACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC 
TGCCGTATCC TCAACCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATC 
GTCATGGTGA AGGAACACCG AGAGCTTGAT CGGACTGGAA CAAGAAAGGG GCACATTGTC 
ATCAAGGGCA CCTCAGAAAG GTTAACGATG CATTTGGTGG AAGAACATTC AGTGGTGGAT 
CCAACGTTCA TAGAAGACTT CCTGTTGACC TATAGGACTT TTCTCTGTAG CCCGATGGAA 
GTGGGCAAAA AGTTGTTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACGCGG 
GTAGTATTAT TGTGGGTGAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT 
CGATTTCTAG AAGAATTTGA AAATAATCTG GAAAGAGAGA AAATGGGTGG ACATCTAAGG 
CTGTTAAATA TTGCATGTGC AGCTAAAGCA AAAAGAAGAC TGATGACGCT AACGAAACCA 
GCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CAGAGAAAGG ATTTGGAATC 
TTTGTTGACA GTGTAGATTC AGCTAGCAAA GCAACAGAAG CGGGCTTGAA ACGAGGGGAT 
CAGATACTAG AAGTAAATGG TCAAAACTTT GAAAATATTC AGCTATCAAA AGCCATGGAA 
ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA 
GAACTTCTAA CAAGATTATC AGAAGAGAAG AGAAATGGTG CCCCTCACCT CCCTAAAATT 
GGAGACATCA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TCGCAGTAGA TGTAGAGCAA 
GTGATAGGAC TTGAAAAAGT AAATAAGAAA AGCAAAGCCA ACACTGTTGG GGGAAGGAAC 
AAGCTCAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATATAAT 
GATATCGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACTAAGCAC 
ATCCCAACTG CACTGCCTGT CAGCGGAACC TTATCGTCCA GTAATCCTGA CTTACTGCAG 
TCACACCATC GCATTTTAGA CTTCAGTGCT ACTCCTGATT TGCCAGATCA AGTGCTAAGG 
GTTTTCAAGG CTGACCAGCA AAGCCGATAC ATCATGATCA GTAAGGACAC TACGGCGAAG 
GAAGTGGTCG TTCAGGCCAT CCGGGAGTTT GCCGTGACCG CCACCCCAGA TCAGTACTCG 
CTCTGTGAAG TCTCTGTCAC ACCAGAGGGA GTGATCAAGC AAAGAAGGCT TCCAGATCAG 
CTTTCCAAAC TTGCTGACAG AATACAGCTG AGCGGAAGGT ATTACTTGAA AAACAACATG 
GAGACGGAAA CCCTTTGCTC GGATGAGGAT GCACAGGAGC TGCTGCGAGA GAGCCAGATC 
TCACTGCTGC AGCTCAGCAC CGTGGAGGTG GCCACGCAGC TCTCCATGCG CAACTTCGAG 
CTTTTCCGCA ACATCGAGCC CACTGAGTAT ATCGATGACT TATTCAAGCT TAAATCAAAA 
ACCAGCTGTG CCAACCTGAA GACATTCGAG GAAGTCATTA ACCAGGAGAC GTTTTGGGTA 
GCATCTGAGA TTCTGCGGGA GACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC 
ATCAAGATAG CTCTGCACTG TAGGGAGTGC AAGAATTTTA ACTCCATGTT TGCGATCATC 
AGTGGCCTAA ACCTGGCGCC GGTGGCAAGA CTGCGAACTA CCTGGGAGAA ACTTCCCAAT 
AAATACGAAA AGCTGTTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA 
AAATACCGCA ATGTCCTCAA CAGTCAGAAC CTGCAGCCTC CTATCATCCC CCTGTTCCCA 
GTGATCAAGA AGGATCTTAC GTTCCTTCAC GAAGGGAATG ACTCCAAGGT TGATGGGCTG 
GTCAATTTTG AGAAGCTAAG AATGATTGCA AAGGAAATAC GTCACGTTGG CCGAATGGCC 
TCTGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAGTG GAGGAGTCTG 
GGGTCGCTCA GCCAGGGTAG TACAAACGCA ACCGTGCTAG ATGTTGCTCA GACAGGTGGG 
CATAAAAAGC GGGTGCGTCG TAGCTCCTTT CTCAATGCCA AAAAGCTATA TGAAGATGCC 
CAGATGGCTC GCAAAGTGAA GCAGTACCTG TCCAATCTGG AGCTCGAGAT GGATGAGGAG 
AGTCTTCAGA CGCTATCCCT GCAGTGTGAG CCGGCCACCA ACACACTGCC TAAGAATCCC 
ACTGACAAAA AGCCTGTCAA ATCGGAGACC TCCCCAGTGG CCCCGAGAGC GGGGTTGCAG 
CCGAAAGCAC AGCCGCAGCC CCAGCCGCCG CAGCCGCCAC ACAAACTCAA CCAGGGACTG 
CAGGTTCCCG CCGTGTCCCT TTACCCTTCA CGGAAGAAAG TTCCCGTAAA GGACCTCCCA 
CCTTTCGGCA TCAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGCCTGGAGC GCCATAGGAG GCCAGCAGAA GACACGATAT CCAACACGTC TTCCCAGCTC 
TCCTCTCCGC CCACCTCCCC ACAGAGCTCC CCACGGAAAG GTGGCAGTGG CAGTCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGATTTA ACCAGCTCCT CCTCTTCTCT TGGAAGTGAG 
AACAGGAACA AGAATAACAA TGCACCACGG ACCTATGGGA TAGGCTACAC TTTGGCTCCG 
AGTGGCACCG TGGATAACTT CTCAGATTCC GGCCACAGTG AAATCTCCTC ACGATCCAGT 
ATCGTCAGCA ACTCCTCCTT CGATTCATTG CCCGTCTCGC TGCCTGACGA GCGGCGCCAG 
AGGCCGTCAG TCAGCATCGT GGAGACCAGC CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG 
GCGGTGGAGC CTGACCAGTA TAGCCTGGGT TCCTGTGCAC CACTGTCTGA GAGCAGGGGC 
CTATATGCTG CAGCCACCGT GATCTCTTCC CCCAGCACGG AGGAGCTTTC CCAGGACCAG 
GGGGACCGAG CCTCCCTGGA CGCTGCTGAC AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG 
AGCGGCTCCC ATGATAACAT CCAGACCATC CAGCACCAGA GGAGCTGGGA GACGCTCCCC 
TTTGGGCACA CTCACTTTGA TTACCCAGGG GATGCTGCAG GGCTGTGGGC CTCGAGCAGC 
CATATGGACC AAATCATGTT TCCCGACCAC AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC 
CGAGAGAGTC TCGATCAGGC CCAGTCCCGG GCCAGCTGGG CCTCGTCCAC AGGGTACTGG 
GGAGAGGACT CGGAAGGGGA CACGGGCACC ATCAAGAGAA GGGGGGGTAA GGACGTTTCC 
CTTGACGCTG ACAGCAGCAG CATGACAGCC GTGACCGCAG AGGAAGCCAA ACCTGCCGCC 
ATGGCTGCCC ACATAGCCGT GACACCCAGT GCTGCCAAGG GGCTCATCGC AGCCAGGAAG 
GAGGGCCGCT ACCGGGAGCC GCCGCCCACT CCACCAGGCT ACGTGGGCAT CCCCATCACT 
GACTTCCCCG AGGCCCACCC GCACCCGGCC CGGAAACCGC CGGACTACAC TGTGGCGCTG 
CAGCGGTCCA GGATGCTGGC CCGGCCGGCC GAACCCCCTG CCCCAGGCAG CGCCAGGCCT 
GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT GATGGTGACC CACGCGCCGG CCCCTGTGCA 
CCCCCGGGCC TCACCGCCGA GGAGGACGCA GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832757.1
CDS	258..4994
Translation	MNECGLPVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976989.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976989.1

ORF Insert Sequence:

ATGAATGAAT GTGGTCTACC CGTAGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGT 
CAAGCTTCTC ATCGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGGGAAAGA 
CAAACTATTA TTGACACTGT GGATCCTTAT CCTGTGGGCA AACCACCTTT GCCTAGAGGC 
TATCATACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAGCT ACACCTCACT 
GACAGTCTCC ACCCACAGGT GACCCACGTC TCCTCCAGCC ACTCAGGATG TAGCATCACT 
AGTGATTCTG GAAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAGAG TGAGGCTGGT 
GATATGGACC TTAGTGGGTT GCCAGAAACA GCAGTGGACT CTGAGGACGA TGATGATGAA 
GAGGACATCG AGAGGGCCTC AGACCCTCTA ATGAGCAGAG ACATCGTGAG AGACTGCCTG 
GAGAAGGACC CCATTGATAG GACAGACGAT GACATTGAAC AACTTCTGGA ATTCATGCAC 
CAGTTGCCTG CTTTTGCCAA CATGACGATG TCGGTGAGGC GGGAGCTGTG TGCTGTGATG 
GTGTTCGCTG TGGTGGAAAG AGCTGGGACC ATCGTGTTAA ACGACGGTGA AGAGCTGGAC 
TCATGGTCAG TGATCCTGAA TGGTTCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA 
ATACTCTGTA TGGGAAATAG TTTCGGTGTC TCCCCCACCA TGGACAAGGA GTACATGAAA 
GGTGTGATGA GGACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC 
TGCCGTATCC TCAACCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATC 
GTCATGGTGA AGGAACACCG AGAGCTTGAT CGGACTGGAA CAAGAAAGGG GCACATTGTC 
ATCAAGGGCA CCTCAGAAAG GTTAACGATG CATTTGGTGG AAGAACATTC AGTGGTGGAT 
CCAACGTTCA TAGAAGACTT CCTGTTGACC TATAGGACTT TTCTCTGTAG CCCGATGGAA 
GTGGGCAAAA AGTTGTTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACGCGG 
GTAGTATTAT TGTGGGTGAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT 
CGATTTCTAG AAGAATTTGA AAATAATCTG GAAAGAGAGA AAATGGGTGG ACATCTAAGG 
CTGTTAAATA TTGCATGTGC AGCTAAAGCA AAAAGAAGAC TGATGACGCT AACGAAACCA 
GCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CAGAGAAAGG ATTTGGAATC 
TTTGTTGACA GTGTAGATTC AGCTAGCAAA GCAACAGAAG CGGGCTTGAA ACGAGGGGAT 
CAGATACTAG AAGTAAATGG TCAAAACTTT GAAAATATTC AGCTATCAAA AGCCATGGAA 
ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA 
GAACTTCTAA CAAGATTATC AGAAGAGAAG AGAAATGGTG CCCCTCACCT CCCTAAAATT 
GGAGACATCA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TCGCAGTAGA TGTAGAGCAA 
GTGATAGGAC TTGAAAAAGT AAATAAGAAA AGCAAAGCCA ACACTGTTGG GGGAAGGAAC 
AAGCTCAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATATAAT 
GATATCGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACTAAGCAC 
ATCCCAACTG CACTGCCTGT CAGCGGAACC TTATCGTCCA GTAATCCTGA CTTACTGCAG 
TCACACCATC GCATTTTAGA CTTCAGTGCT ACTCCTGATT TGCCAGATCA AGTGCTAAGG 
GTTTTCAAGG CTGACCAGCA AAGCCGATAC ATCATGATCA GTAAGGACAC TACGGCGAAG 
GAAGTGGTCG TTCAGGCCAT CCGGGAGTTT GCCGTGACCG CCACCCCAGA TCAGTACTCG 
CTCTGTGAAG TCTCTGTCAC ACCAGAGGGA GTGATCAAGC AAAGAAGGCT TCCAGATCAG 
CTTTCCAAAC TTGCTGACAG AATACAGCTG AGCGGAAGGT ATTACTTGAA AAACAACATG 
GAGACGGAAA CCCTTTGCTC GGATGAGGAT GCACAGGAGC TGCTGCGAGA GAGCCAGATC 
TCACTGCTGC AGCTCAGCAC CGTGGAGGTG GCCACGCAGC TCTCCATGCG CAACTTCGAG 
CTTTTCCGCA ACATCGAGCC CACTGAGTAT ATCGATGACT TATTCAAGCT TAAATCAAAA 
ACCAGCTGTG CCAACCTGAA GACATTCGAG GAAGTCATTA ACCAGGAGAC GTTTTGGGTA 
GCATCTGAGA TTCTGCGGGA GACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC 
ATCAAGATAG CTCTGCACTG TAGGGAGTGC AAGAATTTTA ACTCCATGTT TGCGATCATC 
AGTGGCCTAA ACCTGGCGCC GGTGGCAAGA CTGCGAACTA CCTGGGAGAA ACTTCCCAAT 
AAATACGAAA AGCTGTTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA 
AAATACCGCA ATGTCCTCAA CAGTCAGAAC CTGCAGCCTC CTATCATCCC CCTGTTCCCA 
GTGATCAAGA AGGATCTTAC GTTCCTTCAC GAAGGGAATG ACTCCAAGGT TGATGGGCTG 
GTCAATTTTG AGAAGCTAAG AATGATTGCA AAGGAAATAC GTCACGTTGG CCGAATGGCC 
TCTGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAGTG GAGGAGTCTG 
GGGTCGCTCA GCCAGGGTAG TACAAACGCA ACCGTGCTAG ATGTTGCTCA GACAGGTGGG 
CATAAAAAGC GGGTGCGTCG TAGCTCCTTT CTCAATGCCA AAAAGCTATA TGAAGATGCC 
CAGATGGCTC GCAAAGTGAA GCAGTACCTG TCCAATCTGG AGCTCGAGAT GGATGAGGAG 
AGTCTTCAGA CGCTATCCCT GCAGTGTGAG CCGGCCACCA ACACACTGCC TAAGAATCCC 
ACTGACAAAA AGCCTGTCAA ATCGGAGACC TCCCCAGTGG CCCCGAGAGC GGGGTTGCAG 
CCGAAAGCAC AGCCGCAGCC CCAGCCGCCG CAGCCGCCAC ACAAACTCAA CCAGGGACTG 
CAGGTTCCCG CCGTGTCCCT TTACCCTTCA CGGAAGAAAG TTCCCGTAAA GGACCTCCCA 
CCTTTCGGCA TCAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGCCTGGAGC GCCATAGGAG GCCAGCAGAA GACACGATAT CCAACACGTC TTCCCAGCTC 
TCCTCTCCGC CCACCTCCCC ACAGAGCTCC CCACGGAAAG GTGGCAGTGG CAGTCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGATTTA ACCAGCTCCT CCTCTTCTCT TGGAAGTGAG 
AACAGGAACA AGAATAACAA TGCACCACGG ACCTATGGGA TAGGCTACAC TTTGGCTCCG 
AGTGGCACCG TGGATAACTT CTCAGATTCC GGCCACAGTG AAATCTCCTC ACGATCCAGT 
ATCGTCAGCA ACTCCTCCTT CGATTCATTG CCCGTCTCGC TGCCTGACGA GCGGCGCCAG 
AGGCCGTCAG TCAGCATCGT GGAGACCAGC CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG 
GCGGTGGAGC CTGACCAGTA TAGCCTGGGT TCCTGTGCAC CACTGTCTGA GAGCAGGGGC 
CTATATGCTG CAGCCACCGT GATCTCTTCC CCCAGCACGG AGGAGCTTTC CCAGGACCAG 
GGGGACCGAG CCTCCCTGGA CGCTGCTGAC AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG 
AGCGGCTCCC ATGATAACAT CCAGACCATC CAGCACCAGA GGAGCTGGGA GACGCTCCCC 
TTTGGGCACA CTCACTTTGA TTACCCAGGG GATGCTGCAG GGCTGTGGGC CTCGAGCAGC 
CATATGGACC AAATCATGTT TCCCGACCAC AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC 
CGAGAGAGTC TCGATCAGGC CCAGTCCCGG GCCAGCTGGG CCTCGTCCAC AGGGTACTGG 
GGAGAGGACT CGGAAGGGGA CACGGGCACC ATCAAGAGAA GGGGGGGTAA GGACGTTTCC 
CTTGACGCTG ACAGCAGCAG CATGACAGCC GTGACCGCAG AGGAAGCCAA ACCTGCCGCC 
ATGGCTGCCC ACATAGCCGT GACACCCAGT GCTGCCAAGG GGCTCATCGC AGCCAGGAAG 
GAGGGCCGCT ACCGGGAGCC GCCGCCCACT CCACCAGGCT ACGTGGGCAT CCCCATCACT 
GACTTCCCCG AGGCCCACCC GCACCCGGCC CGGAAACCGC CGGACTACAC TGTGGCGCTG 
CAGCGGTCCA GGATGCTGGC CCGGCCGGCC GAACCCCCTG CCCCAGGCAG CGCCAGGCCT 
GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT GATGGTGACC CACGCGCCGG CCCCTGTGCA 
CCCCCGGGCC TCACCGCCGA GGAGGACGCA GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976992.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832760.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X26, mRNA.

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CloneID	OBa224136
Clone ID Related Accession (Same CDS sequence)	XM_024976992.1
Accession Version	XM_024976992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832760.1
CDS	1..4587
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976992.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976993.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832761.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa224137
Clone ID Related Accession (Same CDS sequence)	XM_024976993.1
Accession Version	XM_024976993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCCAGGA AGGAGGGCCG CTACCGGGAG 
CCGCCGCCCA CTCCACCAGG CTACGTGGGC ATCCCCATCA CTGACTTCCC CGAGGCCCAC 
CCGCACCCGG CCCGGAAACC GCCGGACTAC ACTGTGGCGC TGCAGCGGTC CAGGATGCTG 
GCCCGGCCGG CCGAACCCCC TGCCCCAGGC AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG 
CACCGGCCTG GTGATGGTGA CCCACGCGCC GGCCCCTGTG CACCCCCGGG CCTCACCGCC 
GAGGAGGACG CAGAAGATGA ACAAGTGTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832761.1
CDS	1..4539
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976993.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976993.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCCAGGA AGGAGGGCCG CTACCGGGAG 
CCGCCGCCCA CTCCACCAGG CTACGTGGGC ATCCCCATCA CTGACTTCCC CGAGGCCCAC 
CCGCACCCGG CCCGGAAACC GCCGGACTAC ACTGTGGCGC TGCAGCGGTC CAGGATGCTG 
GCCCGGCCGG CCGAACCCCC TGCCCCAGGC AGCGCCAGGC CTGCCCCCAG GCCGCAGTGG 
CACCGGCCTG GTGATGGTGA CCCACGCGCC GGCCCCTGTG CACCCCCGGG CCTCACCGCC 
GAGGAGGACG CAGAAGATGA ACAAGTGTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024976991.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832759.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa58990
Clone ID Related Accession (Same CDS sequence)	XM_024976991.1
Accession Version	XM_024976991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCAGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832759.1
CDS	200..4801
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_024976991.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976991.1

ORF Insert Sequence:

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGC TACACTTTGG CTCCGAGTGG CACCGTGGAT 
AACTTCTCAG ATTCCGGCCA CAGTGAAATC TCCTCACGAT CCAGTATCGT CAGCAACTCC 
TCCTTCGATT CATTGCCCGT CTCGCTGCCT GACGAGCGGC GCCAGAGGCC GTCAGTCAGC 
ATCGTGGAGA CCAGCCTGGC CTCGGGCCGG CTGGAGAGGC GGCCGGCGGT GGAGCCTGAC 
CAGTATAGCC TGGGTTCCTG TGCACCACTG TCTGAGAGCA GGGGCCTATA TGCTGCAGCC 
ACCGTGATCT CTTCCCCCAG CACGGAGGAG CTTTCCCAGG ACCAGGGGGA CCGAGCCTCC 
CTGGACGCTG CTGACAGTGG CCGGGGGAGC TGGACGTCGT GCTCGAGCGG CTCCCATGAT 
AACATCCAGA CCATCCAGCA CCAGAGGAGC TGGGAGACGC TCCCCTTTGG GCACACTCAC 
TTTGATTACC CAGGGGATGC TGCAGGGCTG TGGGCCTCGA GCAGCCATAT GGACCAAATC 
ATGTTTCCCG ACCACAGTGC AAAGTACAGC AGGCAGAGTC AGAGCCGAGA GAGTCTCGAT 
CAGGCCCAGT CCCGGGCCAG CTGGGCCTCG TCCACAGGGT ACTGGGGAGA GGACTCGGAA 
GGGGACACGG GCACCATCAA GAGAAGGGGG GGTAAGGACG TTTCCCTTGA CGCTGACAGC 
AGCAGCATGA CAGCCGTGAC CGCAGAGGAA GCCAAACCTG CCGCCATGGC TGCCCACATA 
GCCGTGACAC CCAGTGCTGC CAAGGGGCTC ATCGCAGCCA GGAAGGAGGG CCGCTACCGG 
GAGCCGCCGC CCACTCCACC AGGCTACGTG GGCATCCCCA TCACTGACTT CCCCGAGGCC 
CACCCGCACC CGGCCCGGAA ACCGCCGGAC TACACTGTGG CGCTGCAGCG GTCCAGGATG 
CTGGCCCGGC CGGCCGAACC CCCTGCCCCA GGCAGCGCCA GGCCTGCCCC CAGGCCGCAG 
TGGCACCGGC CTGGTGATGG TGACCCACGC GCCGGCCCCT GTGCACCCCC GGGCCTCACC 
GCCGAGGAGG ACGCAGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475406.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330892.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OBa93151
Clone ID Related Accession (Same CDS sequence)	XM_015475406.1
Accession Version	XM_015475406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG TGAGAACAGG 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACTTTGGC TCCGAGTGGC 
ACCGTGGATA ACTTCTCAGA TTCCGGCCAC AGTGAAATCT CCTCACGATC CAGTATCGTC 
AGCAACTCCT CCTTCGATTC ATTGCCCGTC TCGCTGCCTG ACGAGCGGCG CCAGAGGCCG 
TCAGTCAGCA TCGTGGAGAC CAGCCTGGCC TCGGGCCGGC TGGAGAGGCG GCCGGCGGTG 
GAGCCTGACC AGTATAGCCT GGGTTCCTGT GCACCACTGT CTGAGAGCAG GGGCCTATAT 
GCTGCAGCCA CCGTGATCTC TTCCCCCAGC ACGGAGGAGC TTTCCCAGGA CCAGGGGGAC 
CGAGCCTCCC TGGACGCTGC TGACAGTGGC CGGGGGAGCT GGACGTCGTG CTCGAGCGGC 
TCCCATGATA ACATCCAGAC CATCCAGCAC CAGAGGAGCT GGGAGACGCT CCCCTTTGGG 
CACACTCACT TTGATTACCC AGGGGATGCT GCAGGGCTGT GGGCCTCGAG CAGCCATATG 
GACCAAATCA TGTTTCCCGA CCACAGTGCA AAGTACAGCA GGCAGAGTCA GAGCCGAGAG 
AGTCTCGATC AGGCCCAGTC CCGGGCCAGC TGGGCCTCGT CCACAGGGTA CTGGGGAGAG 
GACTCGGAAG GGGACACGGG CACCATCAAG AGAAGGGGGG GTAAGGACGT TTCCCTTGAC 
GCTGACAGCA GCAGCATGAC AGCCGTGACC GCAGAGGAAG CCAAACCTGC CGCCATGGCT 
GCCCACATAG CCGTGACACC CAGTGCTGCC AAGGGGCTCA TCGCAGCCAG GAAGGAGGGC 
CGCTACCGGG AGCCGCCGCC CACTCCACCA GGCTACGTGG GCATCCCCAT CACTGACTTC 
CCCGAGGCCC ACCCGCACCC GGCCCGGAAA CCGCCGGACT ACACTGTGGC GCTGCAGCGG 
TCCAGGATGC TGGCCCGGCC GGCCGAACCC CCTGCCCCAG GCAGCGCCAG GCCTGCCCCC 
AGGCCGCAGT GGCACCGGCC TGGTGATGGT GACCCACGCG CCGGCCCCTG TGCACCCCCG 
GGCCTCACCG CCGAGGAGGA CGCAGAAGAT GAACAAGTGT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330892.1
CDS	63..5033
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475406.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475406.1

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG TGAGAACAGG 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACTTTGGC TCCGAGTGGC 
ACCGTGGATA ACTTCTCAGA TTCCGGCCAC AGTGAAATCT CCTCACGATC CAGTATCGTC 
AGCAACTCCT CCTTCGATTC ATTGCCCGTC TCGCTGCCTG ACGAGCGGCG CCAGAGGCCG 
TCAGTCAGCA TCGTGGAGAC CAGCCTGGCC TCGGGCCGGC TGGAGAGGCG GCCGGCGGTG 
GAGCCTGACC AGTATAGCCT GGGTTCCTGT GCACCACTGT CTGAGAGCAG GGGCCTATAT 
GCTGCAGCCA CCGTGATCTC TTCCCCCAGC ACGGAGGAGC TTTCCCAGGA CCAGGGGGAC 
CGAGCCTCCC TGGACGCTGC TGACAGTGGC CGGGGGAGCT GGACGTCGTG CTCGAGCGGC 
TCCCATGATA ACATCCAGAC CATCCAGCAC CAGAGGAGCT GGGAGACGCT CCCCTTTGGG 
CACACTCACT TTGATTACCC AGGGGATGCT GCAGGGCTGT GGGCCTCGAG CAGCCATATG 
GACCAAATCA TGTTTCCCGA CCACAGTGCA AAGTACAGCA GGCAGAGTCA GAGCCGAGAG 
AGTCTCGATC AGGCCCAGTC CCGGGCCAGC TGGGCCTCGT CCACAGGGTA CTGGGGAGAG 
GACTCGGAAG GGGACACGGG CACCATCAAG AGAAGGGGGG GTAAGGACGT TTCCCTTGAC 
GCTGACAGCA GCAGCATGAC AGCCGTGACC GCAGAGGAAG CCAAACCTGC CGCCATGGCT 
GCCCACATAG CCGTGACACC CAGTGCTGCC AAGGGGCTCA TCGCAGCCAG GAAGGAGGGC 
CGCTACCGGG AGCCGCCGCC CACTCCACCA GGCTACGTGG GCATCCCCAT CACTGACTTC 
CCCGAGGCCC ACCCGCACCC GGCCCGGAAA CCGCCGGACT ACACTGTGGC GCTGCAGCGG 
TCCAGGATGC TGGCCCGGCC GGCCGAACCC CCTGCCCCAG GCAGCGCCAG GCCTGCCCCC 
AGGCCGCAGT GGCACCGGCC TGGTGATGGT GACCCACGCG CCGGCCCCTG TGCACCCCCG 
GGCCTCACCG CCGAGGAGGA CGCAGAAGAT GAACAAGTGT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475407.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330893.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OBa93152
Clone ID Related Accession (Same CDS sequence)	XM_015475407.1
Accession Version	XM_015475407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG TGAGAACAGG 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACTTTGGC TCCGAGTGGC 
ACCGTGGATA ACTTCTCAGA TTCCGGCCAC AGTGAAATCT CCTCACGATC CAGTATCGTC 
AGCAACTCCT CCTTCGATTC ATTGCCCGTC TCGCTGCCTG ACGAGCGGCG CCAGAGGCCG 
TCAGTCAGCA TCGTGGAGAC CAGCCTGGCC TCGGGCCGGC TGGAGAGGCG GCCGGCGGTG 
GAGCCTGACC AGTATAGCCT GGGTTCCTGT GCACCACTGT CTGAGAGCAG GGGCCTATAT 
GCTGCAGCCA CCGTGATCTC TTCCCCCAGC ACGGAGGAGC TTTCCCAGGA CCAGGGGGAC 
CGAGCCTCCC TGGACGCTGC TGACAGTGGC CGGGGGAGCT GGACGTCGTG CTCGAGCGGC 
TCCCATGATA ACATCCAGAC CATCCAGCAC CAGAGGAGCT GGGAGACGCT CCCCTTTGGG 
CACACTCACT TTGATTACCC AGGGGATGCT GCAGGGCTGT GGGCCTCGAG CAGCCATATG 
GACCAAATCA TGTTTCCCGA CCACAGTGCA AAGTACAGCA GGCAGAGTCA GAGCCGAGAG 
AGTCTCGATC AGGCCCAGTC CCGGGCCAGC TGGGCCTCGT CCACAGGGTA CTGGGGAGAG 
GACTCGGAAG GGGACACGGG CACCATCAAG AGAAGGGGGG GTAAGGACGT TTCCCTTGAC 
GCTGACAGCA GCAGCATGAC AGCCGTGACC GCAGAGGAAG CCAAACCTGC CGCCATGGCT 
GCCCACATAG CCGTGACACC CAGTGCTGCC AAGGGGCTCA TCGCCAGGAA GGAGGGCCGC 
TACCGGGAGC CGCCGCCCAC TCCACCAGGC TACGTGGGCA TCCCCATCAC TGACTTCCCC 
GAGGCCCACC CGCACCCGGC CCGGAAACCG CCGGACTACA CTGTGGCGCT GCAGCGGTCC 
AGGATGCTGG CCCGGCCGGC CGAACCCCCT GCCCCAGGCA GCGCCAGGCC TGCCCCCAGG 
CCGCAGTGGC ACCGGCCTGG TGATGGTGAC CCACGCGCCG GCCCCTGTGC ACCCCCGGGC 
CTCACCGCCG AGGAGGACGC AGAAGATGAA CAAGTGTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330893.1
CDS	63..5030
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475407.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475407.1

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG TGAGAACAGG 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACTTTGGC TCCGAGTGGC 
ACCGTGGATA ACTTCTCAGA TTCCGGCCAC AGTGAAATCT CCTCACGATC CAGTATCGTC 
AGCAACTCCT CCTTCGATTC ATTGCCCGTC TCGCTGCCTG ACGAGCGGCG CCAGAGGCCG 
TCAGTCAGCA TCGTGGAGAC CAGCCTGGCC TCGGGCCGGC TGGAGAGGCG GCCGGCGGTG 
GAGCCTGACC AGTATAGCCT GGGTTCCTGT GCACCACTGT CTGAGAGCAG GGGCCTATAT 
GCTGCAGCCA CCGTGATCTC TTCCCCCAGC ACGGAGGAGC TTTCCCAGGA CCAGGGGGAC 
CGAGCCTCCC TGGACGCTGC TGACAGTGGC CGGGGGAGCT GGACGTCGTG CTCGAGCGGC 
TCCCATGATA ACATCCAGAC CATCCAGCAC CAGAGGAGCT GGGAGACGCT CCCCTTTGGG 
CACACTCACT TTGATTACCC AGGGGATGCT GCAGGGCTGT GGGCCTCGAG CAGCCATATG 
GACCAAATCA TGTTTCCCGA CCACAGTGCA AAGTACAGCA GGCAGAGTCA GAGCCGAGAG 
AGTCTCGATC AGGCCCAGTC CCGGGCCAGC TGGGCCTCGT CCACAGGGTA CTGGGGAGAG 
GACTCGGAAG GGGACACGGG CACCATCAAG AGAAGGGGGG GTAAGGACGT TTCCCTTGAC 
GCTGACAGCA GCAGCATGAC AGCCGTGACC GCAGAGGAAG CCAAACCTGC CGCCATGGCT 
GCCCACATAG CCGTGACACC CAGTGCTGCC AAGGGGCTCA TCGCCAGGAA GGAGGGCCGC 
TACCGGGAGC CGCCGCCCAC TCCACCAGGC TACGTGGGCA TCCCCATCAC TGACTTCCCC 
GAGGCCCACC CGCACCCGGC CCGGAAACCG CCGGACTACA CTGTGGCGCT GCAGCGGTCC 
AGGATGCTGG CCCGGCCGGC CGAACCCCCT GCCCCAGGCA GCGCCAGGCC TGCCCCCAGG 
CCGCAGTGGC ACCGGCCTGG TGATGGTGAC CCACGCGCCG GCCCCTGTGC ACCCCCGGGC 
CTCACCGCCG AGGAGGACGC AGAAGATGAA CAAGTGTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475408.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330894.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OBa93153
Clone ID Related Accession (Same CDS sequence)	XM_015475408.1
Accession Version	XM_015475408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGCAGAAG ATGAACAAGT GTCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330894.1
CDS	63..5015
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475408.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475408.1

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGCTTCCTG CAGATTTCAC AAAGCTACAC CTCACTGACA GTCTCCACCC ACAGGTGACC 
CACGTCTCCT CCAGCCACTC AGGATGTAGC ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAGAGTGAG GCTGGTGATA TGGACCTTAG TGGGTTGCCA 
GAAACAGCAG TGGACTCTGA GGACGATGAT GATGAAGAGG ACATCGAGAG GGCCTCAGAC 
CCTCTAATGA GCAGAGACAT CGTGAGAGAC TGCCTGGAGA AGGACCCCAT TGATAGGACA 
GACGATGACA TTGAACAACT TCTGGAATTC ATGCACCAGT TGCCTGCTTT TGCCAACATG 
ACGATGTCGG TGAGGCGGGA GCTGTGTGCT GTGATGGTGT TCGCTGTGGT GGAAAGAGCT 
GGGACCATCG TGTTAAACGA CGGTGAAGAG CTGGACTCAT GGTCAGTGAT CCTGAATGGT 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TCTGTATGGG AAATAGTTTC 
GGTGTCTCCC CCACCATGGA CAAGGAGTAC ATGAAAGGTG TGATGAGGAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATCCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATCGTCA TGGTGAAGGA ACACCGAGAG 
CTTGATCGGA CTGGAACAAG AAAGGGGCAC ATTGTCATCA AGGGCACCTC AGAAAGGTTA 
ACGATGCATT TGGTGGAAGA ACATTCAGTG GTGGATCCAA CGTTCATAGA AGACTTCCTG 
TTGACCTATA GGACTTTTCT CTGTAGCCCG ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACGCGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGACTGAT GACGCTAACG AAACCAGCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCAGA GAAAGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGCT 
AGCAAAGCAA CAGAAGCGGG CTTGAAACGA GGGGATCAGA TACTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT ATCAAAAGCC ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTATCAGAA 
GAGAAGAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTCGC AGTAGATGTA GAGCAAGTGA TAGGACTTGA AAAAGTAAAT 
AAGAAAAGCA AAGCCAACAC TGTTGGGGGA AGGAACAAGC TCAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TCGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACT AAGCACATCC CAACTGCACT GCCTGTCAGC 
GGAACCTTAT CGTCCAGTAA TCCTGACTTA CTGCAGTCAC ACCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGATTTGCC AGATCAAGTG CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACTACG GCGAAGGAAG TGGTCGTTCA GGCCATCCGG 
GAGTTTGCCG TGACCGCCAC CCCAGATCAG TACTCGCTCT GTGAAGTCTC TGTCACACCA 
GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA GATCAGCTTT CCAAACTTGC TGACAGAATA 
CAGCTGAGCG GAAGGTATTA CTTGAAAAAC AACATGGAGA CGGAAACCCT TTGCTCGGAT 
GAGGATGCAC AGGAGCTGCT GCGAGAGAGC CAGATCTCAC TGCTGCAGCT CAGCACCGTG 
GAGGTGGCCA CGCAGCTCTC CATGCGCAAC TTCGAGCTTT TCCGCAACAT CGAGCCCACT 
GAGTATATCG ATGACTTATT CAAGCTTAAA TCAAAAACCA GCTGTGCCAA CCTGAAGACA 
TTCGAGGAAG TCATTAACCA GGAGACGTTT TGGGTAGCAT CTGAGATTCT GCGGGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCTCT GCACTGTAGG 
GAGTGCAAGA ATTTTAACTC CATGTTTGCG ATCATCAGTG GCCTAAACCT GGCGCCGGTG 
GCAAGACTGC GAACTACCTG GGAGAAACTT CCCAATAAAT ACGAAAAGCT GTTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ACCGCAATGT CCTCAACAGT 
CAGAACCTGC AGCCTCCTAT CATCCCCCTG TTCCCAGTGA TCAAGAAGGA TCTTACGTTC 
CTTCACGAAG GGAATGACTC CAAGGTTGAT GGGCTGGTCA ATTTTGAGAA GCTAAGAATG 
ATTGCAAAGG AAATACGTCA CGTTGGCCGA ATGGCCTCTG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG AGTCTGGGGT CGCTCAGCCA GGGTAGTACA 
AACGCAACCG TGCTAGATGT TGCTCAGACA GGTGGGCATA AAAAGCGGGT GCGTCGTAGC 
TCCTTTCTCA ATGCCAAAAA GCTATATGAA GATGCCCAGA TGGCTCGCAA AGTGAAGCAG 
TACCTGTCCA ATCTGGAGCT CGAGATGGAT GAGGAGAGTC TTCAGACGCT ATCCCTGCAG 
TGTGAGCCGG CCACCAACAC ACTGCCTAAG AATCCCACTG ACAAAAAGCC TGTCAAATCG 
GAGACCTCCC CAGTGGCCCC GAGAGCGGGG TTGCAGCCGA AAGCACAGCC GCAGCCCCAG 
CCGCCGCAGC CGCCACACAA ACTCAACCAG GGACTGCAGG TTCCCGCCGT GTCCCTTTAC 
CCTTCACGGA AGAAAGTTCC CGTAAAGGAC CTCCCACCTT TCGGCATCAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA 
GCAGAAGACA CGATATCCAA CACGTCTTCC CAGCTCTCCT CTCCGCCCAC CTCCCCACAG 
AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GATTTAACCA GCTCCTCCTC TTCTCTTGGA AGTGAGAACA GGAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACTTTG GCTCCGAGTG GCACCGTGGA TAACTTCTCA 
GATTCCGGCC ACAGTGAAAT CTCCTCACGA TCCAGTATCG TCAGCAACTC CTCCTTCGAT 
TCATTGCCCG TCTCGCTGCC TGACGAGCGG CGCCAGAGGC CGTCAGTCAG CATCGTGGAG 
ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG CGGCCGGCGG TGGAGCCTGA CCAGTATAGC 
CTGGGTTCCT GTGCACCACT GTCTGAGAGC AGGGGCCTAT ATGCTGCAGC CACCGTGATC 
TCTTCCCCCA GCACGGAGGA GCTTTCCCAG GACCAGGGGG ACCGAGCCTC CCTGGACGCT 
GCTGACAGTG GCCGGGGGAG CTGGACGTCG TGCTCGAGCG GCTCCCATGA TAACATCCAG 
ACCATCCAGC ACCAGAGGAG CTGGGAGACG CTCCCCTTTG GGCACACTCA CTTTGATTAC 
CCAGGGGATG CTGCAGGGCT GTGGGCCTCG AGCAGCCATA TGGACCAAAT CATGTTTCCC 
GACCACAGTG CAAAGTACAG CAGGCAGAGT CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG 
TCCCGGGCCA GCTGGGCCTC GTCCACAGGG TACTGGGGAG AGGACTCGGA AGGGGACACG 
GGCACCATCA AGAGAAGGGG GGGTAAGGAC GTTTCCCTTG ACGCTGACAG CAGCAGCATG 
ACAGCCGTGA CCGCAGAGGA AGCCAAACCT GCCGCCATGG CTGCCCACAT AGCCGTGACA 
CCCAGTGCTG CCAAGGGGCT CATCGCAGCC AGGAAGGAGG GCCGCTACCG GGAGCCGCCG 
CCCACTCCAC CAGGCTACGT GGGCATCCCC ATCACTGACT TCCCCGAGGC CCACCCGCAC 
CCGGCCCGGA AACCGCCGGA CTACACTGTG GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG 
CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC AGGCCTGCCC CCAGGCCGCA GTGGCACCGG 
CCTGGTGATG GTGACCCACG CGCCGGCCCC TGTGCACCCC CGGGCCTCAC CGCCGAGGAG 
GACGCAGAAG ATGAACAAGT GTCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475409.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330895.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OBa93154
Clone ID Related Accession (Same CDS sequence)	XM_015475409.1
Accession Version	XM_015475409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGTCGCTCA GCCAGGGTAG TACAAACGCA 
ACCGTGCTAG ATGTTGCTCA GACAGGTGGG CATAAAAAGC GGGTGCGTCG TAGCTCCTTT 
CTCAATGCCA AAAAGCTATA TGAAGATGCC CAGATGGCTC GCAAAGTGAA GCAGTACCTG 
TCCAATCTGG AGCTCGAGAT GGATGAGGAG AGTCTTCAGA CGCTATCCCT GCAGTGTGAG 
CCGGCCACCA ACACACTGCC TAAGAATCCC ACTGACAAAA AGCCTGTCAA ATCGGAGACC 
TCCCCAGTGG CCCCGAGAGC GGGGTTGCAG CCGAAAGCAC AGCCGCAGCC CCAGCCGCCG 
CAGCCGCCAC ACAAACTCAA CCAGGGACTG CAGGTTCCCG CCGTGTCCCT TTACCCTTCA 
CGGAAGAAAG TTCCCGTAAA GGACCTCCCA CCTTTCGGCA TCAACTCTCC ACAAGCTTTA 
AAGAAAATTC TTTCTTTGTC TGAAGAAGGA AGCCTGGAGC GCCATAGGAG GCCAGCAGAA 
GACACGATAT CCAACACGTC TTCCCAGCTC TCCTCTCCGC CCACCTCCCC ACAGAGCTCC 
CCACGGAAAG GTGGCAGTGG CAGTCAGCTG AGATCTTTTG GCTCCGGGCA GTTGGATTTA 
ACCAGCTCCT CCTCTTCTCT TGGAAGTGAG AACAGGAACA AGAATAACAA TGCACCACGG 
ACCTATGGGA TAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC AGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330895.1
CDS	63..5009
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475409.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475409.1

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGTCGCTCA GCCAGGGTAG TACAAACGCA 
ACCGTGCTAG ATGTTGCTCA GACAGGTGGG CATAAAAAGC GGGTGCGTCG TAGCTCCTTT 
CTCAATGCCA AAAAGCTATA TGAAGATGCC CAGATGGCTC GCAAAGTGAA GCAGTACCTG 
TCCAATCTGG AGCTCGAGAT GGATGAGGAG AGTCTTCAGA CGCTATCCCT GCAGTGTGAG 
CCGGCCACCA ACACACTGCC TAAGAATCCC ACTGACAAAA AGCCTGTCAA ATCGGAGACC 
TCCCCAGTGG CCCCGAGAGC GGGGTTGCAG CCGAAAGCAC AGCCGCAGCC CCAGCCGCCG 
CAGCCGCCAC ACAAACTCAA CCAGGGACTG CAGGTTCCCG CCGTGTCCCT TTACCCTTCA 
CGGAAGAAAG TTCCCGTAAA GGACCTCCCA CCTTTCGGCA TCAACTCTCC ACAAGCTTTA 
AAGAAAATTC TTTCTTTGTC TGAAGAAGGA AGCCTGGAGC GCCATAGGAG GCCAGCAGAA 
GACACGATAT CCAACACGTC TTCCCAGCTC TCCTCTCCGC CCACCTCCCC ACAGAGCTCC 
CCACGGAAAG GTGGCAGTGG CAGTCAGCTG AGATCTTTTG GCTCCGGGCA GTTGGATTTA 
ACCAGCTCCT CCTCTTCTCT TGGAAGTGAG AACAGGAACA AGAATAACAA TGCACCACGG 
ACCTATGGGA TAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC AGCCAGGAAG GAGGGCCGCT ACCGGGAGCC GCCGCCCACT 
CCACCAGGCT ACGTGGGCAT CCCCATCACT GACTTCCCCG AGGCCCACCC GCACCCGGCC 
CGGAAACCGC CGGACTACAC TGTGGCGCTG CAGCGGTCCA GGATGCTGGC CCGGCCGGCC 
GAACCCCCTG CCCCAGGCAG CGCCAGGCCT GCCCCCAGGC CGCAGTGGCA CCGGCCTGGT 
GATGGTGACC CACGCGCCGG CCCCTGTGCA CCCCCGGGCC TCACCGCCGA GGAGGACGCA 
GAAGATGAAC AAGTGTCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475411.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330897.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa93155
Clone ID Related Accession (Same CDS sequence)	XM_015475411.1
Accession Version	XM_015475411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330897.1
CDS	63..4985
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475411.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475411.1

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGTGGCA GTGGCAGTCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA TTTAACCAGC TCCTCCTCTT CTCTTGGAAG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475412.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330898.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa93156
Clone ID Related Accession (Same CDS sequence)	XM_015475412.1 , XM_015475412.2
Accession Version	XM_015475412.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTGTTAGAGC CTTCTGAAAT GATTGTGGTA GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGG 
GAAAGACAAA CTATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
AGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAGCTACAC 
CTCACTGACA GTCTCCACCC ACAGGTGACC CACGTCTCCT CCAGCCACTC AGGATGTAGC 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAGAGTGAG 
GCTGGTGATA TGGACCTTAG TGGGTTGCCA GAAACAGCAG TGGACTCTGA GGACGATGAT 
GATGAAGAGG ACATCGAGAG GGCCTCAGAC CCTCTAATGA GCAGAGACAT CGTGAGAGAC 
TGCCTGGAGA AGGACCCCAT TGATAGGACA GACGATGACA TTGAACAACT TCTGGAATTC 
ATGCACCAGT TGCCTGCTTT TGCCAACATG ACGATGTCGG TGAGGCGGGA GCTGTGTGCT 
GTGATGGTGT TCGCTGTGGT GGAAAGAGCT GGGACCATCG TGTTAAACGA CGGTGAAGAG 
CTGGACTCAT GGTCAGTGAT CCTGAATGGT TCTGTGGAAG TGACTTATCC AGATGGAAAA 
GCAGAAATAC TCTGTATGGG AAATAGTTTC GGTGTCTCCC CCACCATGGA CAAGGAGTAC 
ATGAAAGGTG TGATGAGGAC AAAGGTGGAT GACTGCCAGT TTGTCTGCAT AGCCCAGCAA 
GATTACTGCC GTATCCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA AGAGGAAGGA 
GAGATCGTCA TGGTGAAGGA ACACCGAGAG CTTGATCGGA CTGGAACAAG AAAGGGGCAC 
ATTGTCATCA AGGGCACCTC AGAAAGGTTA ACGATGCATT TGGTGGAAGA ACATTCAGTG 
GTGGATCCAA CGTTCATAGA AGACTTCCTG TTGACCTATA GGACTTTTCT CTGTAGCCCG 
ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACGCGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AATCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGACTGAT GACGCTAACG 
AAACCAGCCC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GAGGCTCAGA GAAAGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGCT AGCAAAGCAA CAGAAGCGGG CTTGAAACGA 
GGGGATCAGA TACTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT ATCAAAAGCC 
ATGGAAATTC TTAGAAATAA CACACATTTA TCTATCACTG TGAAAACCAA TTTATTTGTA 
TTTAAAGAAC TTCTAACAAG ATTATCAGAA GAGAAGAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTCGC AGTAGATGTA 
GAGCAAGTGA TAGGACTTGA AAAAGTAAAT AAGAAAAGCA AAGCCAACAC TGTTGGGGGA 
AGGAACAAGC TCAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TCGGGATTGG TCAGTCTCAA GATGACAGCA TAGTAGGATT AAGGCAGACT 
AAGCACATCC CAACTGCACT GCCTGTCAGC GGAACCTTAT CGTCCAGTAA TCCTGACTTA 
CTGCAGTCAC ACCATCGCAT TTTAGACTTC AGTGCTACTC CTGATTTGCC AGATCAAGTG 
CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC CGATACATCA TGATCAGTAA GGACACTACG 
GCGAAGGAAG TGGTCGTTCA GGCCATCCGG GAGTTTGCCG TGACCGCCAC CCCAGATCAG 
TACTCGCTCT GTGAAGTCTC TGTCACACCA GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGACAGAATA CAGCTGAGCG GAAGGTATTA CTTGAAAAAC 
AACATGGAGA CGGAAACCCT TTGCTCGGAT GAGGATGCAC AGGAGCTGCT GCGAGAGAGC 
CAGATCTCAC TGCTGCAGCT CAGCACCGTG GAGGTGGCCA CGCAGCTCTC CATGCGCAAC 
TTCGAGCTTT TCCGCAACAT CGAGCCCACT GAGTATATCG ATGACTTATT CAAGCTTAAA 
TCAAAAACCA GCTGTGCCAA CCTGAAGACA TTCGAGGAAG TCATTAACCA GGAGACGTTT 
TGGGTAGCAT CTGAGATTCT GCGGGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATAGCTCT GCACTGTAGG GAGTGCAAGA ATTTTAACTC CATGTTTGCG 
ATCATCAGTG GCCTAAACCT GGCGCCGGTG GCAAGACTGC GAACTACCTG GGAGAAACTT 
CCCAATAAAT ACGAAAAGCT GTTTCAAGAT CTCCAAGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ACCGCAATGT CCTCAACAGT CAGAACCTGC AGCCTCCTAT CATCCCCCTG 
TTCCCAGTGA TCAAGAAGGA TCTTACGTTC CTTCACGAAG GGAATGACTC CAAGGTTGAT 
GGGCTGGTCA ATTTTGAGAA GCTAAGAATG ATTGCAAAGG AAATACGTCA CGTTGGCCGA 
ATGGCCTCTG TGAACATGGA CCCTGCCCTC ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG 
AGTCTGGGGT CGCTCAGCCA GGGTAGTACA AACGCAACCG TGCTAGATGT TGCTCAGACA 
GGTGGGCATA AAAAGCGGGT GCGTCGTAGC TCCTTTCTCA ATGCCAAAAA GCTATATGAA 
GATGCCCAGA TGGCTCGCAA AGTGAAGCAG TACCTGTCCA ATCTGGAGCT CGAGATGGAT 
GAGGAGAGTC TTCAGACGCT ATCCCTGCAG TGTGAGCCGG CCACCAACAC ACTGCCTAAG 
AATCCCACTG ACAAAAAGCC TGTCAAATCG GAGACCTCCC CAGTGGCCCC GAGAGCGGGG 
TTGCAGCCGA AAGCACAGCC GCAGCCCCAG CCGCCGCAGC CGCCACACAA ACTCAACCAG 
GGACTGCAGG TTCCCGCCGT GTCCCTTTAC CCTTCACGGA AGAAAGTTCC CGTAAAGGAC 
CTCCCACCTT TCGGCATCAA CTCTCCACAA GCTTTAAAGA AAATTCTTTC TTTGTCTGAA 
GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA GCAGAAGACA CGATATCCAA CACGTCTTCC 
CAGCTCTCCT CTCCGCCCAC CTCCCCACAG AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT 
CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG GATTTAACCA GCTCCTCCTC TTCTCTTGGA 
AGTGAGAACA GGAACAAGAA TAACAATGCA CCACGGACCT ATGGGATAGG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330898.1
CDS	140..4942
Translation	MLQSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475412.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475412.1

ORF Insert Sequence:

ATGCTTCAGA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTGTTAGAGC CTTCTGAAAT GATTGTGGTA GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGG 
GAAAGACAAA CTATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
AGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAGCTACAC 
CTCACTGACA GTCTCCACCC ACAGGTGACC CACGTCTCCT CCAGCCACTC AGGATGTAGC 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAGAGTGAG 
GCTGGTGATA TGGACCTTAG TGGGTTGCCA GAAACAGCAG TGGACTCTGA GGACGATGAT 
GATGAAGAGG ACATCGAGAG GGCCTCAGAC CCTCTAATGA GCAGAGACAT CGTGAGAGAC 
TGCCTGGAGA AGGACCCCAT TGATAGGACA GACGATGACA TTGAACAACT TCTGGAATTC 
ATGCACCAGT TGCCTGCTTT TGCCAACATG ACGATGTCGG TGAGGCGGGA GCTGTGTGCT 
GTGATGGTGT TCGCTGTGGT GGAAAGAGCT GGGACCATCG TGTTAAACGA CGGTGAAGAG 
CTGGACTCAT GGTCAGTGAT CCTGAATGGT TCTGTGGAAG TGACTTATCC AGATGGAAAA 
GCAGAAATAC TCTGTATGGG AAATAGTTTC GGTGTCTCCC CCACCATGGA CAAGGAGTAC 
ATGAAAGGTG TGATGAGGAC AAAGGTGGAT GACTGCCAGT TTGTCTGCAT AGCCCAGCAA 
GATTACTGCC GTATCCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA AGAGGAAGGA 
GAGATCGTCA TGGTGAAGGA ACACCGAGAG CTTGATCGGA CTGGAACAAG AAAGGGGCAC 
ATTGTCATCA AGGGCACCTC AGAAAGGTTA ACGATGCATT TGGTGGAAGA ACATTCAGTG 
GTGGATCCAA CGTTCATAGA AGACTTCCTG TTGACCTATA GGACTTTTCT CTGTAGCCCG 
ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACGCGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AATCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGACTGAT GACGCTAACG 
AAACCAGCCC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GAGGCTCAGA GAAAGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGCT AGCAAAGCAA CAGAAGCGGG CTTGAAACGA 
GGGGATCAGA TACTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT ATCAAAAGCC 
ATGGAAATTC TTAGAAATAA CACACATTTA TCTATCACTG TGAAAACCAA TTTATTTGTA 
TTTAAAGAAC TTCTAACAAG ATTATCAGAA GAGAAGAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTCGC AGTAGATGTA 
GAGCAAGTGA TAGGACTTGA AAAAGTAAAT AAGAAAAGCA AAGCCAACAC TGTTGGGGGA 
AGGAACAAGC TCAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TCGGGATTGG TCAGTCTCAA GATGACAGCA TAGTAGGATT AAGGCAGACT 
AAGCACATCC CAACTGCACT GCCTGTCAGC GGAACCTTAT CGTCCAGTAA TCCTGACTTA 
CTGCAGTCAC ACCATCGCAT TTTAGACTTC AGTGCTACTC CTGATTTGCC AGATCAAGTG 
CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC CGATACATCA TGATCAGTAA GGACACTACG 
GCGAAGGAAG TGGTCGTTCA GGCCATCCGG GAGTTTGCCG TGACCGCCAC CCCAGATCAG 
TACTCGCTCT GTGAAGTCTC TGTCACACCA GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGACAGAATA CAGCTGAGCG GAAGGTATTA CTTGAAAAAC 
AACATGGAGA CGGAAACCCT TTGCTCGGAT GAGGATGCAC AGGAGCTGCT GCGAGAGAGC 
CAGATCTCAC TGCTGCAGCT CAGCACCGTG GAGGTGGCCA CGCAGCTCTC CATGCGCAAC 
TTCGAGCTTT TCCGCAACAT CGAGCCCACT GAGTATATCG ATGACTTATT CAAGCTTAAA 
TCAAAAACCA GCTGTGCCAA CCTGAAGACA TTCGAGGAAG TCATTAACCA GGAGACGTTT 
TGGGTAGCAT CTGAGATTCT GCGGGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATAGCTCT GCACTGTAGG GAGTGCAAGA ATTTTAACTC CATGTTTGCG 
ATCATCAGTG GCCTAAACCT GGCGCCGGTG GCAAGACTGC GAACTACCTG GGAGAAACTT 
CCCAATAAAT ACGAAAAGCT GTTTCAAGAT CTCCAAGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ACCGCAATGT CCTCAACAGT CAGAACCTGC AGCCTCCTAT CATCCCCCTG 
TTCCCAGTGA TCAAGAAGGA TCTTACGTTC CTTCACGAAG GGAATGACTC CAAGGTTGAT 
GGGCTGGTCA ATTTTGAGAA GCTAAGAATG ATTGCAAAGG AAATACGTCA CGTTGGCCGA 
ATGGCCTCTG TGAACATGGA CCCTGCCCTC ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG 
AGTCTGGGGT CGCTCAGCCA GGGTAGTACA AACGCAACCG TGCTAGATGT TGCTCAGACA 
GGTGGGCATA AAAAGCGGGT GCGTCGTAGC TCCTTTCTCA ATGCCAAAAA GCTATATGAA 
GATGCCCAGA TGGCTCGCAA AGTGAAGCAG TACCTGTCCA ATCTGGAGCT CGAGATGGAT 
GAGGAGAGTC TTCAGACGCT ATCCCTGCAG TGTGAGCCGG CCACCAACAC ACTGCCTAAG 
AATCCCACTG ACAAAAAGCC TGTCAAATCG GAGACCTCCC CAGTGGCCCC GAGAGCGGGG 
TTGCAGCCGA AAGCACAGCC GCAGCCCCAG CCGCCGCAGC CGCCACACAA ACTCAACCAG 
GGACTGCAGG TTCCCGCCGT GTCCCTTTAC CCTTCACGGA AGAAAGTTCC CGTAAAGGAC 
CTCCCACCTT TCGGCATCAA CTCTCCACAA GCTTTAAAGA AAATTCTTTC TTTGTCTGAA 
GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA GCAGAAGACA CGATATCCAA CACGTCTTCC 
CAGCTCTCCT CTCCGCCCAC CTCCCCACAG AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT 
CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG GATTTAACCA GCTCCTCCTC TTCTCTTGGA 
AGTGAGAACA GGAACAAGAA TAACAATGCA CCACGGACCT ATGGGATAGG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475412.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330898.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X20, mRNA.

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CloneID	OBa93156
Clone ID Related Accession (Same CDS sequence)	XM_015475412.1 , XM_015475412.2
Accession Version	XM_015475412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTGTTAGAGC CTTCTGAAAT GATTGTGGTA GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGG 
GAAAGACAAA CTATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
AGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAGCTACAC 
CTCACTGACA GTCTCCACCC ACAGGTGACC CACGTCTCCT CCAGCCACTC AGGATGTAGC 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAGAGTGAG 
GCTGGTGATA TGGACCTTAG TGGGTTGCCA GAAACAGCAG TGGACTCTGA GGACGATGAT 
GATGAAGAGG ACATCGAGAG GGCCTCAGAC CCTCTAATGA GCAGAGACAT CGTGAGAGAC 
TGCCTGGAGA AGGACCCCAT TGATAGGACA GACGATGACA TTGAACAACT TCTGGAATTC 
ATGCACCAGT TGCCTGCTTT TGCCAACATG ACGATGTCGG TGAGGCGGGA GCTGTGTGCT 
GTGATGGTGT TCGCTGTGGT GGAAAGAGCT GGGACCATCG TGTTAAACGA CGGTGAAGAG 
CTGGACTCAT GGTCAGTGAT CCTGAATGGT TCTGTGGAAG TGACTTATCC AGATGGAAAA 
GCAGAAATAC TCTGTATGGG AAATAGTTTC GGTGTCTCCC CCACCATGGA CAAGGAGTAC 
ATGAAAGGTG TGATGAGGAC AAAGGTGGAT GACTGCCAGT TTGTCTGCAT AGCCCAGCAA 
GATTACTGCC GTATCCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA AGAGGAAGGA 
GAGATCGTCA TGGTGAAGGA ACACCGAGAG CTTGATCGGA CTGGAACAAG AAAGGGGCAC 
ATTGTCATCA AGGGCACCTC AGAAAGGTTA ACGATGCATT TGGTGGAAGA ACATTCAGTG 
GTGGATCCAA CGTTCATAGA AGACTTCCTG TTGACCTATA GGACTTTTCT CTGTAGCCCG 
ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACGCGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AATCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGACTGAT GACGCTAACG 
AAACCAGCCC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GAGGCTCAGA GAAAGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGCT AGCAAAGCAA CAGAAGCGGG CTTGAAACGA 
GGGGATCAGA TACTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT ATCAAAAGCC 
ATGGAAATTC TTAGAAATAA CACACATTTA TCTATCACTG TGAAAACCAA TTTATTTGTA 
TTTAAAGAAC TTCTAACAAG ATTATCAGAA GAGAAGAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTCGC AGTAGATGTA 
GAGCAAGTGA TAGGACTTGA AAAAGTAAAT AAGAAAAGCA AAGCCAACAC TGTTGGGGGA 
AGGAACAAGC TCAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TCGGGATTGG TCAGTCTCAA GATGACAGCA TAGTAGGATT AAGGCAGACT 
AAGCACATCC CAACTGCACT GCCTGTCAGC GGAACCTTAT CGTCCAGTAA TCCTGACTTA 
CTGCAGTCAC ACCATCGCAT TTTAGACTTC AGTGCTACTC CTGATTTGCC AGATCAAGTG 
CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC CGATACATCA TGATCAGTAA GGACACTACG 
GCGAAGGAAG TGGTCGTTCA GGCCATCCGG GAGTTTGCCG TGACCGCCAC CCCAGATCAG 
TACTCGCTCT GTGAAGTCTC TGTCACACCA GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGACAGAATA CAGCTGAGCG GAAGGTATTA CTTGAAAAAC 
AACATGGAGA CGGAAACCCT TTGCTCGGAT GAGGATGCAC AGGAGCTGCT GCGAGAGAGC 
CAGATCTCAC TGCTGCAGCT CAGCACCGTG GAGGTGGCCA CGCAGCTCTC CATGCGCAAC 
TTCGAGCTTT TCCGCAACAT CGAGCCCACT GAGTATATCG ATGACTTATT CAAGCTTAAA 
TCAAAAACCA GCTGTGCCAA CCTGAAGACA TTCGAGGAAG TCATTAACCA GGAGACGTTT 
TGGGTAGCAT CTGAGATTCT GCGGGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATAGCTCT GCACTGTAGG GAGTGCAAGA ATTTTAACTC CATGTTTGCG 
ATCATCAGTG GCCTAAACCT GGCGCCGGTG GCAAGACTGC GAACTACCTG GGAGAAACTT 
CCCAATAAAT ACGAAAAGCT GTTTCAAGAT CTCCAAGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ACCGCAATGT CCTCAACAGT CAGAACCTGC AGCCTCCTAT CATCCCCCTG 
TTCCCAGTGA TCAAGAAGGA TCTTACGTTC CTTCACGAAG GGAATGACTC CAAGGTTGAT 
GGGCTGGTCA ATTTTGAGAA GCTAAGAATG ATTGCAAAGG AAATACGTCA CGTTGGCCGA 
ATGGCCTCTG TGAACATGGA CCCTGCCCTC ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG 
AGTCTGGGGT CGCTCAGCCA GGGTAGTACA AACGCAACCG TGCTAGATGT TGCTCAGACA 
GGTGGGCATA AAAAGCGGGT GCGTCGTAGC TCCTTTCTCA ATGCCAAAAA GCTATATGAA 
GATGCCCAGA TGGCTCGCAA AGTGAAGCAG TACCTGTCCA ATCTGGAGCT CGAGATGGAT 
GAGGAGAGTC TTCAGACGCT ATCCCTGCAG TGTGAGCCGG CCACCAACAC ACTGCCTAAG 
AATCCCACTG ACAAAAAGCC TGTCAAATCG GAGACCTCCC CAGTGGCCCC GAGAGCGGGG 
TTGCAGCCGA AAGCACAGCC GCAGCCCCAG CCGCCGCAGC CGCCACACAA ACTCAACCAG 
GGACTGCAGG TTCCCGCCGT GTCCCTTTAC CCTTCACGGA AGAAAGTTCC CGTAAAGGAC 
CTCCCACCTT TCGGCATCAA CTCTCCACAA GCTTTAAAGA AAATTCTTTC TTTGTCTGAA 
GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA GCAGAAGACA CGATATCCAA CACGTCTTCC 
CAGCTCTCCT CTCCGCCCAC CTCCCCACAG AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT 
CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG GATTTAACCA GCTCCTCCTC TTCTCTTGGA 
AGTGAGAACA GGAACAAGAA TAACAATGCA CCACGGACCT ATGGGATAGG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330898.1
CDS	134..4936
Translation	MLQSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475412.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475412.2

ORF Insert Sequence:

ATGCTTCAGA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTGTTAGAGC CTTCTGAAAT GATTGTGGTA GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGG 
GAAAGACAAA CTATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
AGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAGCTACAC 
CTCACTGACA GTCTCCACCC ACAGGTGACC CACGTCTCCT CCAGCCACTC AGGATGTAGC 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAGAGTGAG 
GCTGGTGATA TGGACCTTAG TGGGTTGCCA GAAACAGCAG TGGACTCTGA GGACGATGAT 
GATGAAGAGG ACATCGAGAG GGCCTCAGAC CCTCTAATGA GCAGAGACAT CGTGAGAGAC 
TGCCTGGAGA AGGACCCCAT TGATAGGACA GACGATGACA TTGAACAACT TCTGGAATTC 
ATGCACCAGT TGCCTGCTTT TGCCAACATG ACGATGTCGG TGAGGCGGGA GCTGTGTGCT 
GTGATGGTGT TCGCTGTGGT GGAAAGAGCT GGGACCATCG TGTTAAACGA CGGTGAAGAG 
CTGGACTCAT GGTCAGTGAT CCTGAATGGT TCTGTGGAAG TGACTTATCC AGATGGAAAA 
GCAGAAATAC TCTGTATGGG AAATAGTTTC GGTGTCTCCC CCACCATGGA CAAGGAGTAC 
ATGAAAGGTG TGATGAGGAC AAAGGTGGAT GACTGCCAGT TTGTCTGCAT AGCCCAGCAA 
GATTACTGCC GTATCCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA AGAGGAAGGA 
GAGATCGTCA TGGTGAAGGA ACACCGAGAG CTTGATCGGA CTGGAACAAG AAAGGGGCAC 
ATTGTCATCA AGGGCACCTC AGAAAGGTTA ACGATGCATT TGGTGGAAGA ACATTCAGTG 
GTGGATCCAA CGTTCATAGA AGACTTCCTG TTGACCTATA GGACTTTTCT CTGTAGCCCG 
ATGGAAGTGG GCAAAAAGTT GTTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACGCGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AATCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGACTGAT GACGCTAACG 
AAACCAGCCC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GAGGCTCAGA GAAAGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGCT AGCAAAGCAA CAGAAGCGGG CTTGAAACGA 
GGGGATCAGA TACTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT ATCAAAAGCC 
ATGGAAATTC TTAGAAATAA CACACATTTA TCTATCACTG TGAAAACCAA TTTATTTGTA 
TTTAAAGAAC TTCTAACAAG ATTATCAGAA GAGAAGAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTCGC AGTAGATGTA 
GAGCAAGTGA TAGGACTTGA AAAAGTAAAT AAGAAAAGCA AAGCCAACAC TGTTGGGGGA 
AGGAACAAGC TCAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TCGGGATTGG TCAGTCTCAA GATGACAGCA TAGTAGGATT AAGGCAGACT 
AAGCACATCC CAACTGCACT GCCTGTCAGC GGAACCTTAT CGTCCAGTAA TCCTGACTTA 
CTGCAGTCAC ACCATCGCAT TTTAGACTTC AGTGCTACTC CTGATTTGCC AGATCAAGTG 
CTAAGGGTTT TCAAGGCTGA CCAGCAAAGC CGATACATCA TGATCAGTAA GGACACTACG 
GCGAAGGAAG TGGTCGTTCA GGCCATCCGG GAGTTTGCCG TGACCGCCAC CCCAGATCAG 
TACTCGCTCT GTGAAGTCTC TGTCACACCA GAGGGAGTGA TCAAGCAAAG AAGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGACAGAATA CAGCTGAGCG GAAGGTATTA CTTGAAAAAC 
AACATGGAGA CGGAAACCCT TTGCTCGGAT GAGGATGCAC AGGAGCTGCT GCGAGAGAGC 
CAGATCTCAC TGCTGCAGCT CAGCACCGTG GAGGTGGCCA CGCAGCTCTC CATGCGCAAC 
TTCGAGCTTT TCCGCAACAT CGAGCCCACT GAGTATATCG ATGACTTATT CAAGCTTAAA 
TCAAAAACCA GCTGTGCCAA CCTGAAGACA TTCGAGGAAG TCATTAACCA GGAGACGTTT 
TGGGTAGCAT CTGAGATTCT GCGGGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATAGCTCT GCACTGTAGG GAGTGCAAGA ATTTTAACTC CATGTTTGCG 
ATCATCAGTG GCCTAAACCT GGCGCCGGTG GCAAGACTGC GAACTACCTG GGAGAAACTT 
CCCAATAAAT ACGAAAAGCT GTTTCAAGAT CTCCAAGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ACCGCAATGT CCTCAACAGT CAGAACCTGC AGCCTCCTAT CATCCCCCTG 
TTCCCAGTGA TCAAGAAGGA TCTTACGTTC CTTCACGAAG GGAATGACTC CAAGGTTGAT 
GGGCTGGTCA ATTTTGAGAA GCTAAGAATG ATTGCAAAGG AAATACGTCA CGTTGGCCGA 
ATGGCCTCTG TGAACATGGA CCCTGCCCTC ATGTTCAGGA CTCGGAAGAA GAAGTGGAGG 
AGTCTGGGGT CGCTCAGCCA GGGTAGTACA AACGCAACCG TGCTAGATGT TGCTCAGACA 
GGTGGGCATA AAAAGCGGGT GCGTCGTAGC TCCTTTCTCA ATGCCAAAAA GCTATATGAA 
GATGCCCAGA TGGCTCGCAA AGTGAAGCAG TACCTGTCCA ATCTGGAGCT CGAGATGGAT 
GAGGAGAGTC TTCAGACGCT ATCCCTGCAG TGTGAGCCGG CCACCAACAC ACTGCCTAAG 
AATCCCACTG ACAAAAAGCC TGTCAAATCG GAGACCTCCC CAGTGGCCCC GAGAGCGGGG 
TTGCAGCCGA AAGCACAGCC GCAGCCCCAG CCGCCGCAGC CGCCACACAA ACTCAACCAG 
GGACTGCAGG TTCCCGCCGT GTCCCTTTAC CCTTCACGGA AGAAAGTTCC CGTAAAGGAC 
CTCCCACCTT TCGGCATCAA CTCTCCACAA GCTTTAAAGA AAATTCTTTC TTTGTCTGAA 
GAAGGAAGCC TGGAGCGCCA TAGGAGGCCA GCAGAAGACA CGATATCCAA CACGTCTTCC 
CAGCTCTCCT CTCCGCCCAC CTCCCCACAG AGCTCCCCAC GGAAAGGTGG CAGTGGCAGT 
CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG GATTTAACCA GCTCCTCCTC TTCTCTTGGA 
AGTGAGAACA GGAACAAGAA TAACAATGCA CCACGGACCT ATGGGATAGG CTACACTTTG 
GCTCCGAGTG GCACCGTGGA TAACTTCTCA GATTCCGGCC ACAGTGAAAT CTCCTCACGA 
TCCAGTATCG TCAGCAACTC CTCCTTCGAT TCATTGCCCG TCTCGCTGCC TGACGAGCGG 
CGCCAGAGGC CGTCAGTCAG CATCGTGGAG ACCAGCCTGG CCTCGGGCCG GCTGGAGAGG 
CGGCCGGCGG TGGAGCCTGA CCAGTATAGC CTGGGTTCCT GTGCACCACT GTCTGAGAGC 
AGGGGCCTAT ATGCTGCAGC CACCGTGATC TCTTCCCCCA GCACGGAGGA GCTTTCCCAG 
GACCAGGGGG ACCGAGCCTC CCTGGACGCT GCTGACAGTG GCCGGGGGAG CTGGACGTCG 
TGCTCGAGCG GCTCCCATGA TAACATCCAG ACCATCCAGC ACCAGAGGAG CTGGGAGACG 
CTCCCCTTTG GGCACACTCA CTTTGATTAC CCAGGGGATG CTGCAGGGCT GTGGGCCTCG 
AGCAGCCATA TGGACCAAAT CATGTTTCCC GACCACAGTG CAAAGTACAG CAGGCAGAGT 
CAGAGCCGAG AGAGTCTCGA TCAGGCCCAG TCCCGGGCCA GCTGGGCCTC GTCCACAGGG 
TACTGGGGAG AGGACTCGGA AGGGGACACG GGCACCATCA AGAGAAGGGG GGGTAAGGAC 
GTTTCCCTTG ACGCTGACAG CAGCAGCATG ACAGCCGTGA CCGCAGAGGA AGCCAAACCT 
GCCGCCATGG CTGCCCACAT AGCCGTGACA CCCAGTGCTG CCAAGGGGCT CATCGCAGCC 
AGGAAGGAGG GCCGCTACCG GGAGCCGCCG CCCACTCCAC CAGGCTACGT GGGCATCCCC 
ATCACTGACT TCCCCGAGGC CCACCCGCAC CCGGCCCGGA AACCGCCGGA CTACACTGTG 
GCGCTGCAGC GGTCCAGGAT GCTGGCCCGG CCGGCCGAAC CCCCTGCCCC AGGCAGCGCC 
AGGCCTGCCC CCAGGCCGCA GTGGCACCGG CCTGGTGATG GTGACCCACG CGCCGGCCCC 
TGTGCACCCC CGGGCCTCAC CGCCGAGGAG GACGCAGAAG ATGAACAAGT GTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475414.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330900.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

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CloneID	OBa93157
Clone ID Related Accession (Same CDS sequence)	XM_015475414.1 , XM_015475413.2 , XM_015475414.2 , XM_015475413.1
Accession Version	XM_015475414.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330900.1
CDS	235..4959
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475414.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475414.1

ORF Insert Sequence:

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475413.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330899.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X22, mRNA.

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CloneID	OBa93157
Clone ID Related Accession (Same CDS sequence)	XM_015475414.1 , XM_015475413.2 , XM_015475414.2 , XM_015475413.1
Accession Version	XM_015475413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330899.1
CDS	379..5103
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475413.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475413.2

ORF Insert Sequence:

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475414.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330900.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X23, mRNA.

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CloneID	OBa93157
Clone ID Related Accession (Same CDS sequence)	XM_015475414.1 , XM_015475413.2 , XM_015475414.2 , XM_015475413.1
Accession Version	XM_015475414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330900.1
CDS	230..4954
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475414.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475414.2

ORF Insert Sequence:

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475413.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330899.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa93157
Clone ID Related Accession (Same CDS sequence)	XM_015475414.1 , XM_015475413.2 , XM_015475414.2 , XM_015475413.1
Accession Version	XM_015475413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330899.1
CDS	694..5418
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475413.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475413.1

ORF Insert Sequence:

ATGATTGTGG TAGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GGGAAAGACA AACTATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTAGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAGCTAC ACCTCACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTCTC CTCCAGCCAC TCAGGATGTA GCATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAGAGTG AGGCTGGTGA TATGGACCTT 
AGTGGGTTGC CAGAAACAGC AGTGGACTCT GAGGACGATG ATGATGAAGA GGACATCGAG 
AGGGCCTCAG ACCCTCTAAT GAGCAGAGAC ATCGTGAGAG ACTGCCTGGA GAAGGACCCC 
ATTGATAGGA CAGACGATGA CATTGAACAA CTTCTGGAAT TCATGCACCA GTTGCCTGCT 
TTTGCCAACA TGACGATGTC GGTGAGGCGG GAGCTGTGTG CTGTGATGGT GTTCGCTGTG 
GTGGAAAGAG CTGGGACCAT CGTGTTAAAC GACGGTGAAG AGCTGGACTC ATGGTCAGTG 
ATCCTGAATG GTTCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTCTGTATG 
GGAAATAGTT TCGGTGTCTC CCCCACCATG GACAAGGAGT ACATGAAAGG TGTGATGAGG 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATCCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATCGT CATGGTGAAG 
GAACACCGAG AGCTTGATCG GACTGGAACA AGAAAGGGGC ACATTGTCAT CAAGGGCACC 
TCAGAAAGGT TAACGATGCA TTTGGTGGAA GAACATTCAG TGGTGGATCC AACGTTCATA 
GAAGACTTCC TGTTGACCTA TAGGACTTTT CTCTGTAGCC CGATGGAAGT GGGCAAAAAG 
TTGTTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACGCGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGACTG ATGACGCTAA CGAAACCAGC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCA GAGAAAGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG CTAGCAAAGC AACAGAAGCG GGCTTGAAAC GAGGGGATCA GATACTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTATCAAAAG CCATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTATCAG AAGAGAAGAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTC GCAGTAGATG TAGAGCAAGT GATAGGACTT 
GAAAAAGTAA ATAAGAAAAG CAAAGCCAAC ACTGTTGGGG GAAGGAACAA GCTCAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATCGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CTAAGCACAT CCCAACTGCA 
CTGCCTGTCA GCGGAACCTT ATCGTCCAGT AATCCTGACT TACTGCAGTC ACACCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGATTTG CCAGATCAAG TGCTAAGGGT TTTCAAGGCT 
GACCAGCAAA GCCGATACAT CATGATCAGT AAGGACACTA CGGCGAAGGA AGTGGTCGTT 
CAGGCCATCC GGGAGTTTGC CGTGACCGCC ACCCCAGATC AGTACTCGCT CTGTGAAGTC 
TCTGTCACAC CAGAGGGAGT GATCAAGCAA AGAAGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGACAGAA TACAGCTGAG CGGAAGGTAT TACTTGAAAA ACAACATGGA GACGGAAACC 
CTTTGCTCGG ATGAGGATGC ACAGGAGCTG CTGCGAGAGA GCCAGATCTC ACTGCTGCAG 
CTCAGCACCG TGGAGGTGGC CACGCAGCTC TCCATGCGCA ACTTCGAGCT TTTCCGCAAC 
ATCGAGCCCA CTGAGTATAT CGATGACTTA TTCAAGCTTA AATCAAAAAC CAGCTGTGCC 
AACCTGAAGA CATTCGAGGA AGTCATTAAC CAGGAGACGT TTTGGGTAGC ATCTGAGATT 
CTGCGGGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCT 
CTGCACTGTA GGGAGTGCAA GAATTTTAAC TCCATGTTTG CGATCATCAG TGGCCTAAAC 
CTGGCGCCGG TGGCAAGACT GCGAACTACC TGGGAGAAAC TTCCCAATAA ATACGAAAAG 
CTGTTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATACCGCAAT 
GTCCTCAACA GTCAGAACCT GCAGCCTCCT ATCATCCCCC TGTTCCCAGT GATCAAGAAG 
GATCTTACGT TCCTTCACGA AGGGAATGAC TCCAAGGTTG ATGGGCTGGT CAATTTTGAG 
AAGCTAAGAA TGATTGCAAA GGAAATACGT CACGTTGGCC GAATGGCCTC TGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAGTGGA GGAGTCTGGG GTCGCTCAGC 
CAGGGTAGTA CAAACGCAAC CGTGCTAGAT GTTGCTCAGA CAGGTGGGCA TAAAAAGCGG 
GTGCGTCGTA GCTCCTTTCT CAATGCCAAA AAGCTATATG AAGATGCCCA GATGGCTCGC 
AAAGTGAAGC AGTACCTGTC CAATCTGGAG CTCGAGATGG ATGAGGAGAG TCTTCAGACG 
CTATCCCTGC AGTGTGAGCC GGCCACCAAC ACACTGCCTA AGAATCCCAC TGACAAAAAG 
CCTGTCAAAT CGGAGACCTC CCCAGTGGCC CCGAGAGCGG GGTTGCAGCC GAAAGCACAG 
CCGCAGCCCC AGCCGCCGCA GCCGCCACAC AAACTCAACC AGGGACTGCA GGTTCCCGCC 
GTGTCCCTTT ACCCTTCACG GAAGAAAGTT CCCGTAAAGG ACCTCCCACC TTTCGGCATC 
AACTCTCCAC AAGCTTTAAA GAAAATTCTT TCTTTGTCTG AAGAAGGAAG CCTGGAGCGC 
CATAGGAGGC CAGCAGAAGA CACGATATCC AACACGTCTT CCCAGCTCTC CTCTCCGCCC 
ACCTCCCCAC AGAGCTCCCC ACGGAAAGGT GGCAGTGGCA GTCAGCTGAG ATCTTTTGGC 
TCCGGGCAGT TGGATTTAAC CAGCTCCTCC TCTTCTCTTG GAAGTGAGAA CAGGAACAAG 
AATAACAATG CACCACGGAC CTATGGGATA GGCTACACTT TGGCTCCGAG TGGCACCGTG 
GATAACTTCT CAGATTCCGG CCACAGTGAA ATCTCCTCAC GATCCAGTAT CGTCAGCAAC 
TCCTCCTTCG ATTCATTGCC CGTCTCGCTG CCTGACGAGC GGCGCCAGAG GCCGTCAGTC 
AGCATCGTGG AGACCAGCCT GGCCTCGGGC CGGCTGGAGA GGCGGCCGGC GGTGGAGCCT 
GACCAGTATA GCCTGGGTTC CTGTGCACCA CTGTCTGAGA GCAGGGGCCT ATATGCTGCA 
GCCACCGTGA TCTCTTCCCC CAGCACGGAG GAGCTTTCCC AGGACCAGGG GGACCGAGCC 
TCCCTGGACG CTGCTGACAG TGGCCGGGGG AGCTGGACGT CGTGCTCGAG CGGCTCCCAT 
GATAACATCC AGACCATCCA GCACCAGAGG AGCTGGGAGA CGCTCCCCTT TGGGCACACT 
CACTTTGATT ACCCAGGGGA TGCTGCAGGG CTGTGGGCCT CGAGCAGCCA TATGGACCAA 
ATCATGTTTC CCGACCACAG TGCAAAGTAC AGCAGGCAGA GTCAGAGCCG AGAGAGTCTC 
GATCAGGCCC AGTCCCGGGC CAGCTGGGCC TCGTCCACAG GGTACTGGGG AGAGGACTCG 
GAAGGGGACA CGGGCACCAT CAAGAGAAGG GGGGGTAAGG ACGTTTCCCT TGACGCTGAC 
AGCAGCAGCA TGACAGCCGT GACCGCAGAG GAAGCCAAAC CTGCCGCCAT GGCTGCCCAC 
ATAGCCGTGA CACCCAGTGC TGCCAAGGGG CTCATCGCAG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475415.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330901.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa93158
Clone ID Related Accession (Same CDS sequence)	XM_015475415.1 , XM_015475415.2
Accession Version	XM_015475415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330901.1
CDS	800..5389
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475415.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475415.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_015475415.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330901.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X25, mRNA.

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CloneID	OBa93158
Clone ID Related Accession (Same CDS sequence)	XM_015475415.1 , XM_015475415.2
Accession Version	XM_015475415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330901.1
CDS	630..5219
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGGSGSQLRSFGSGQLDLTSSSSSLGSENRNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_015475415.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475415.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGTGGCAG TGGCAGTCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAT 
TTAACCAGCT CCTCCTCTTC TCTTGGAAGT GAGAACAGGA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACTTTGGCT CCGAGTGGCA CCGTGGATAA CTTCTCAGAT 
TCCGGCCACA GTGAAATCTC CTCACGATCC AGTATCGTCA GCAACTCCTC CTTCGATTCA 
TTGCCCGTCT CGCTGCCTGA CGAGCGGCGC CAGAGGCCGT CAGTCAGCAT CGTGGAGACC 
AGCCTGGCCT CGGGCCGGCT GGAGAGGCGG CCGGCGGTGG AGCCTGACCA GTATAGCCTG 
GGTTCCTGTG CACCACTGTC TGAGAGCAGG GGCCTATATG CTGCAGCCAC CGTGATCTCT 
TCCCCCAGCA CGGAGGAGCT TTCCCAGGAC CAGGGGGACC GAGCCTCCCT GGACGCTGCT 
GACAGTGGCC GGGGGAGCTG GACGTCGTGC TCGAGCGGCT CCCATGATAA CATCCAGACC 
ATCCAGCACC AGAGGAGCTG GGAGACGCTC CCCTTTGGGC ACACTCACTT TGATTACCCA 
GGGGATGCTG CAGGGCTGTG GGCCTCGAGC AGCCATATGG ACCAAATCAT GTTTCCCGAC 
CACAGTGCAA AGTACAGCAG GCAGAGTCAG AGCCGAGAGA GTCTCGATCA GGCCCAGTCC 
CGGGCCAGCT GGGCCTCGTC CACAGGGTAC TGGGGAGAGG ACTCGGAAGG GGACACGGGC 
ACCATCAAGA GAAGGGGGGG TAAGGACGTT TCCCTTGACG CTGACAGCAG CAGCATGACA 
GCCGTGACCG CAGAGGAAGC CAAACCTGCC GCCATGGCTG CCCACATAGC CGTGACACCC 
AGTGCTGCCA AGGGGCTCAT CGCAGCCAGG AAGGAGGGCC GCTACCGGGA GCCGCCGCCC 
ACTCCACCAG GCTACGTGGG CATCCCCATC ACTGACTTCC CCGAGGCCCA CCCGCACCCG 
GCCCGGAAAC CGCCGGACTA CACTGTGGCG CTGCAGCGGT CCAGGATGCT GGCCCGGCCG 
GCCGAACCCC CTGCCCCAGG CAGCGCCAGG CCTGCCCCCA GGCCGCAGTG GCACCGGCCT 
GGTGATGGTG ACCCACGCGC CGGCCCCTGT GCACCCCCGG GCCTCACCGC CGAGGAGGAC 
GCAGAAGATG AACAAGTGTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001191326.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178255.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), mRNA.

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CloneID	OBa01279
Clone ID Related Accession (Same CDS sequence)	NM_001191326.1
Accession Version	NM_001191326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Jul 17, 2010 this sequence version replaced XM_001789194.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC CAGGAAGGAG GGCCGCTACC GGGAGCCGCC GCCCACTCCA 
CCAGGCTACG TGGGCATCCC CATCACTGAC TTCCCCGAGG CCCACCCGCA CCCGGCCCGG 
AAACCGCCGG ACTACACTGT GGCGCTGCAG CGGTCCAGGA TGCTGGCCCG GCCGGCCGAA 
CCCCCTGCCC CAGGCAGCGC CAGGCCTGCC CCCAGGCCGC AGTGGCACCG GCCTGGTGAT 
GGTGACCCAC GCGCCGGCCC CTGTGCACCC CCGGGCCTCA CCGCCGAGGA GGACGAAGAT 
GAACAAGTGT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178255.1
CDS	1..4461
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001191326.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191326.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC CAGGAAGGAG GGCCGCTACC GGGAGCCGCC GCCCACTCCA 
CCAGGCTACG TGGGCATCCC CATCACTGAC TTCCCCGAGG CCCACCCGCA CCCGGCCCGG 
AAACCGCCGG ACTACACTGT GGCGCTGCAG CGGTCCAGGA TGCTGGCCCG GCCGGCCGAA 
CCCCCTGCCC CAGGCAGCGC CAGGCCTGCC CCCAGGCCGC AGTGGCACCG GCCTGGTGAT 
GGTGACCCAC GCGCCGGCCC CTGTGCACCC CCGGGCCTCA CCGCCGAGGA GGACGAAGAT 
GAACAAGTGT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005217619.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217676.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OBa27714
Clone ID Related Accession (Same CDS sequence)	XM_005217619.3
Accession Version	XM_005217619.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC CAGGAAGGAG GGCCGCTACC GGGAGCCGCC GCCCACTCCA 
CCAGGCTACG TGGGCATCCC CATCACTGAC TTCCCCGAGG CCCACCCGCA CCCGGCCCGG 
AAACCGCCGG ACTACACTGT GGCGCTGCAG CGGTCCAGGA TGCTGGCCCG GCCGGCCGAA 
CCCCCTGCCC CAGGCAGCGC CAGGCCTGCC CCCAGGCCGC AGTGGCACCG GCCTGGTGAT 
GGTGACCCAC GCGCCGGCCC CTGTGCACCC CCGGGCCTCA CCGCCGAGGA GGACGCAGAA 
GATGAACAAG TGTCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217676.1
CDS	1..4464
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_005217619.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217619.3

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA GCTACACCTC ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTCTCCTCCA GCCACTCAGG ATGTAGCATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA GAGTGAGGCT GGTGATATGG ACCTTAGTGG GTTGCCAGAA 
ACAGCAGTGG ACTCTGAGGA CGATGATGAT GAAGAGGACA TCGAGAGGGC CTCAGACCCT 
CTAATGAGCA GAGACATCGT GAGAGACTGC CTGGAGAAGG ACCCCATTGA TAGGACAGAC 
GATGACATTG AACAACTTCT GGAATTCATG CACCAGTTGC CTGCTTTTGC CAACATGACG 
ATGTCGGTGA GGCGGGAGCT GTGTGCTGTG ATGGTGTTCG CTGTGGTGGA AAGAGCTGGG 
ACCATCGTGT TAAACGACGG TGAAGAGCTG GACTCATGGT CAGTGATCCT GAATGGTTCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTCT GTATGGGAAA TAGTTTCGGT 
GTCTCCCCCA CCATGGACAA GGAGTACATG AAAGGTGTGA TGAGGACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TCCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATCGTCATGG TGAAGGAACA CCGAGAGCTT 
GATCGGACTG GAACAAGAAA GGGGCACATT GTCATCAAGG GCACCTCAGA AAGGTTAACG 
ATGCATTTGG TGGAAGAACA TTCAGTGGTG GATCCAACGT TCATAGAAGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTCTG TAGCCCGATG GAAGTGGGCA AAAAGTTGTT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACG CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAT 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GACTGATGAC GCTAACGAAA CCAGCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCAGAGAA AGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGCTAGC 
AAAGCAACAG AAGCGGGCTT GAAACGAGGG GATCAGATAC TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTATC AAAAGCCATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT ATCAGAAGAG 
AAGAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTCGCAGT AGATGTAGAG CAAGTGATAG GACTTGAAAA AGTAAATAAG 
AAAAGCAAAG CCAACACTGT TGGGGGAAGG AACAAGCTCA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATCG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACTAAG CACATCCCAA CTGCACTGCC TGTCAGCGGA 
ACCTTATCGT CCAGTAATCC TGACTTACTG CAGTCACACC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ATTTGCCAGA TCAAGTGCTA AGGGTTTTCA AGGCTGACCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACTACGGCG AAGGAAGTGG TCGTTCAGGC CATCCGGGAG 
TTTGCCGTGA CCGCCACCCC AGATCAGTAC TCGCTCTGTG AAGTCTCTGT CACACCAGAG 
GGAGTGATCA AGCAAAGAAG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA CAGAATACAG 
CTGAGCGGAA GGTATTACTT GAAAAACAAC ATGGAGACGG AAACCCTTTG CTCGGATGAG 
GATGCACAGG AGCTGCTGCG AGAGAGCCAG ATCTCACTGC TGCAGCTCAG CACCGTGGAG 
GTGGCCACGC AGCTCTCCAT GCGCAACTTC GAGCTTTTCC GCAACATCGA GCCCACTGAG 
TATATCGATG ACTTATTCAA GCTTAAATCA AAAACCAGCT GTGCCAACCT GAAGACATTC 
GAGGAAGTCA TTAACCAGGA GACGTTTTGG GTAGCATCTG AGATTCTGCG GGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCTCTGCA CTGTAGGGAG 
TGCAAGAATT TTAACTCCAT GTTTGCGATC ATCAGTGGCC TAAACCTGGC GCCGGTGGCA 
AGACTGCGAA CTACCTGGGA GAAACTTCCC AATAAATACG AAAAGCTGTT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATACC GCAATGTCCT CAACAGTCAG 
AACCTGCAGC CTCCTATCAT CCCCCTGTTC CCAGTGATCA AGAAGGATCT TACGTTCCTT 
CACGAAGGGA ATGACTCCAA GGTTGATGGG CTGGTCAATT TTGAGAAGCT AAGAATGATT 
GCAAAGGAAA TACGTCACGT TGGCCGAATG GCCTCTGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA GTGGAGGAGT CTGGGGTCGC TCAGCCAGGG TAGTACAAAC 
GCAACCGTGC TAGATGTTGC TCAGACAGGT GGGCATAAAA AGCGGGTGCG TCGTAGCTCC 
TTTCTCAATG CCAAAAAGCT ATATGAAGAT GCCCAGATGG CTCGCAAAGT GAAGCAGTAC 
CTGTCCAATC TGGAGCTCGA GATGGATGAG GAGAGTCTTC AGACGCTATC CCTGCAGTGT 
GAGCCGGCCA CCAACACACT GCCTAAGAAT CCCACTGACA AAAAGCCTGT CAAATCGGAG 
ACCTCCCCAG TGGCCCCGAG AGCGGGGTTG CAGCCGAAAG CACAGCCGCA GCCCCAGCCG 
CCGCAGCCGC CACACAAACT CAACCAGGGA CTGCAGGTTC CCGCCGTGTC CCTTTACCCT 
TCACGGAAGA AAGTTCCCGT AAAGGACCTC CCACCTTTCG GCATCAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGCCTGG AGCGCCATAG GAGGCCAGCA 
GAAGACACGA TATCCAACAC GTCTTCCCAG CTCTCCTCTC CGCCCACCTC CCCACAGAGC 
TCCCCACGGA AAGGCTACAC TTTGGCTCCG AGTGGCACCG TGGATAACTT CTCAGATTCC 
GGCCACAGTG AAATCTCCTC ACGATCCAGT ATCGTCAGCA ACTCCTCCTT CGATTCATTG 
CCCGTCTCGC TGCCTGACGA GCGGCGCCAG AGGCCGTCAG TCAGCATCGT GGAGACCAGC 
CTGGCCTCGG GCCGGCTGGA GAGGCGGCCG GCGGTGGAGC CTGACCAGTA TAGCCTGGGT 
TCCTGTGCAC CACTGTCTGA GAGCAGGGGC CTATATGCTG CAGCCACCGT GATCTCTTCC 
CCCAGCACGG AGGAGCTTTC CCAGGACCAG GGGGACCGAG CCTCCCTGGA CGCTGCTGAC 
AGTGGCCGGG GGAGCTGGAC GTCGTGCTCG AGCGGCTCCC ATGATAACAT CCAGACCATC 
CAGCACCAGA GGAGCTGGGA GACGCTCCCC TTTGGGCACA CTCACTTTGA TTACCCAGGG 
GATGCTGCAG GGCTGTGGGC CTCGAGCAGC CATATGGACC AAATCATGTT TCCCGACCAC 
AGTGCAAAGT ACAGCAGGCA GAGTCAGAGC CGAGAGAGTC TCGATCAGGC CCAGTCCCGG 
GCCAGCTGGG CCTCGTCCAC AGGGTACTGG GGAGAGGACT CGGAAGGGGA CACGGGCACC 
ATCAAGAGAA GGGGGGGTAA GGACGTTTCC CTTGACGCTG ACAGCAGCAG CATGACAGCC 
GTGACCGCAG AGGAAGCCAA ACCTGCCGCC ATGGCTGCCC ACATAGCCGT GACACCCAGT 
GCTGCCAAGG GGCTCATCGC CAGGAAGGAG GGCCGCTACC GGGAGCCGCC GCCCACTCCA 
CCAGGCTACG TGGGCATCCC CATCACTGAC TTCCCCGAGG CCCACCCGCA CCCGGCCCGG 
AAACCGCCGG ACTACACTGT GGCGCTGCAG CGGTCCAGGA TGCTGGCCCG GCCGGCCGAA 
CCCCCTGCCC CAGGCAGCGC CAGGCCTGCC CCCAGGCCGC AGTGGCACCG GCCTGGTGAT 
GGTGACCCAC GCGCCGGCCC CTGTGCACCC CCGGGCCTCA CCGCCGAGGA GGACGCAGAA 
GATGAACAAG TGTCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_010813791.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812093.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa58983
Clone ID Related Accession (Same CDS sequence)	XM_010813791.2
Accession Version	XM_010813791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CAGCCAGGAA GGAGGGCCGC 
TACCGGGAGC CGCCGCCCAC TCCACCAGGC TACGTGGGCA TCCCCATCAC TGACTTCCCC 
GAGGCCCACC CGCACCCGGC CCGGAAACCG CCGGACTACA CTGTGGCGCT GCAGCGGTCC 
AGGATGCTGG CCCGGCCGGC CGAACCCCCT GCCCCAGGCA GCGCCAGGCC TGCCCCCAGG 
CCGCAGTGGC ACCGGCCTGG TGATGGTGAC CCACGCGCCG GCCCCTGTGC ACCCCCGGGC 
CTCACCGCCG AGGAGGACGC AGAAGATGAA CAAGTGTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812093.1
CDS	63..4910
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIAARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_010813791.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813791.2

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CAGCCAGGAA GGAGGGCCGC 
TACCGGGAGC CGCCGCCCAC TCCACCAGGC TACGTGGGCA TCCCCATCAC TGACTTCCCC 
GAGGCCCACC CGCACCCGGC CCGGAAACCG CCGGACTACA CTGTGGCGCT GCAGCGGTCC 
AGGATGCTGG CCCGGCCGGC CGAACCCCCT GCCCCAGGCA GCGCCAGGCC TGCCCCCAGG 
CCGCAGTGGC ACCGGCCTGG TGATGGTGAC CCACGCGCCG GCCCCTGTGC ACCCCCGGGC 
CTCACCGCCG AGGAGGACGC AGAAGATGAA CAAGTGTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_010813792.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812094.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

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CloneID	OBa58984
Clone ID Related Accession (Same CDS sequence)	XM_010813792.2
Accession Version	XM_010813792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812094.1
CDS	63..4907
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDAEDEQVSAV

Target ORF information:

RefSeq Version	XM_010813792.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813792.2

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGCAGA AGATGAACAA GTGTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_010813793.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812095.1 Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

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CloneID	OBa58985
Clone ID Related Accession (Same CDS sequence)	XM_010813793.2
Accession Version	XM_010813793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812095.1
CDS	63..4904
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFILLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQPPQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLGSCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPGDAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTAVTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAEPPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_010813793.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813793.2

ORF Insert Sequence:

ATGGAAGCCT TGTCCAACCT GAGAGAGCAT CAGCTTAGGT TGATGTGTGA AACTGTGAGA 
TACGAAAGAC ATGAAGCCAA TGAGGTTTTG TACTATCCTG ATGATATTGG GACCTGCTGG 
TACATCCTTC TCTCCGGTTC CGTGTTCATC AAGGAATCCA TGTTTCTTCC ACGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT GTTAGAGCCT 
TCTGAAATGA TTGTGGTAGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGGGA AAGACAAACT 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTAG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AGCTACACCT CACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTCTCCTCC AGCCACTCAG GATGTAGCAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AGAGTGAGGC TGGTGATATG 
GACCTTAGTG GGTTGCCAGA AACAGCAGTG GACTCTGAGG ACGATGATGA TGAAGAGGAC 
ATCGAGAGGG CCTCAGACCC TCTAATGAGC AGAGACATCG TGAGAGACTG CCTGGAGAAG 
GACCCCATTG ATAGGACAGA CGATGACATT GAACAACTTC TGGAATTCAT GCACCAGTTG 
CCTGCTTTTG CCAACATGAC GATGTCGGTG AGGCGGGAGC TGTGTGCTGT GATGGTGTTC 
GCTGTGGTGG AAAGAGCTGG GACCATCGTG TTAAACGACG GTGAAGAGCT GGACTCATGG 
TCAGTGATCC TGAATGGTTC TGTGGAAGTG ACTTATCCAG ATGGAAAAGC AGAAATACTC 
TGTATGGGAA ATAGTTTCGG TGTCTCCCCC ACCATGGACA AGGAGTACAT GAAAGGTGTG 
ATGAGGACAA AGGTGGATGA CTGCCAGTTT GTCTGCATAG CCCAGCAAGA TTACTGCCGT 
ATCCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAAG AGGAAGGAGA GATCGTCATG 
GTGAAGGAAC ACCGAGAGCT TGATCGGACT GGAACAAGAA AGGGGCACAT TGTCATCAAG 
GGCACCTCAG AAAGGTTAAC GATGCATTTG GTGGAAGAAC ATTCAGTGGT GGATCCAACG 
TTCATAGAAG ACTTCCTGTT GACCTATAGG ACTTTTCTCT GTAGCCCGAT GGAAGTGGGC 
AAAAAGTTGT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC GCGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA TCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGACTGATGA CGCTAACGAA ACCAGCCCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGA GGCTCAGAGA AAGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGCTAG CAAAGCAACA GAAGCGGGCT TGAAACGAGG GGATCAGATA 
CTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTAT CAAAAGCCAT GGAAATTCTT 
AGAAATAACA CACATTTATC TATCACTGTG AAAACCAATT TATTTGTATT TAAAGAACTT 
CTAACAAGAT TATCAGAAGA GAAGAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTCGCAG TAGATGTAGA GCAAGTGATA 
GGACTTGAAA AAGTAAATAA GAAAAGCAAA GCCAACACTG TTGGGGGAAG GAACAAGCTC 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATC 
GGGATTGGTC AGTCTCAAGA TGACAGCATA GTAGGATTAA GGCAGACTAA GCACATCCCA 
ACTGCACTGC CTGTCAGCGG AACCTTATCG TCCAGTAATC CTGACTTACT GCAGTCACAC 
CATCGCATTT TAGACTTCAG TGCTACTCCT GATTTGCCAG ATCAAGTGCT AAGGGTTTTC 
AAGGCTGACC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACTACGGC GAAGGAAGTG 
GTCGTTCAGG CCATCCGGGA GTTTGCCGTG ACCGCCACCC CAGATCAGTA CTCGCTCTGT 
GAAGTCTCTG TCACACCAGA GGGAGTGATC AAGCAAAGAA GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ACAGAATACA GCTGAGCGGA AGGTATTACT TGAAAAACAA CATGGAGACG 
GAAACCCTTT GCTCGGATGA GGATGCACAG GAGCTGCTGC GAGAGAGCCA GATCTCACTG 
CTGCAGCTCA GCACCGTGGA GGTGGCCACG CAGCTCTCCA TGCGCAACTT CGAGCTTTTC 
CGCAACATCG AGCCCACTGA GTATATCGAT GACTTATTCA AGCTTAAATC AAAAACCAGC 
TGTGCCAACC TGAAGACATT CGAGGAAGTC ATTAACCAGG AGACGTTTTG GGTAGCATCT 
GAGATTCTGC GGGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATAGCTCTGC ACTGTAGGGA GTGCAAGAAT TTTAACTCCA TGTTTGCGAT CATCAGTGGC 
CTAAACCTGG CGCCGGTGGC AAGACTGCGA ACTACCTGGG AGAAACTTCC CAATAAATAC 
GAAAAGCTGT TTCAAGATCT CCAAGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAC 
CGCAATGTCC TCAACAGTCA GAACCTGCAG CCTCCTATCA TCCCCCTGTT CCCAGTGATC 
AAGAAGGATC TTACGTTCCT TCACGAAGGG AATGACTCCA AGGTTGATGG GCTGGTCAAT 
TTTGAGAAGC TAAGAATGAT TGCAAAGGAA ATACGTCACG TTGGCCGAAT GGCCTCTGTG 
AACATGGACC CTGCCCTCAT GTTCAGGACT CGGAAGAAGA AGTGGAGGAG TCTGGGGTCG 
CTCAGCCAGG GTAGTACAAA CGCAACCGTG CTAGATGTTG CTCAGACAGG TGGGCATAAA 
AAGCGGGTGC GTCGTAGCTC CTTTCTCAAT GCCAAAAAGC TATATGAAGA TGCCCAGATG 
GCTCGCAAAG TGAAGCAGTA CCTGTCCAAT CTGGAGCTCG AGATGGATGA GGAGAGTCTT 
CAGACGCTAT CCCTGCAGTG TGAGCCGGCC ACCAACACAC TGCCTAAGAA TCCCACTGAC 
AAAAAGCCTG TCAAATCGGA GACCTCCCCA GTGGCCCCGA GAGCGGGGTT GCAGCCGAAA 
GCACAGCCGC AGCCCCAGCC GCCGCAGCCG CCACACAAAC TCAACCAGGG ACTGCAGGTT 
CCCGCCGTGT CCCTTTACCC TTCACGGAAG AAAGTTCCCG TAAAGGACCT CCCACCTTTC 
GGCATCAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGCCTG 
GAGCGCCATA GGAGGCCAGC AGAAGACACG ATATCCAACA CGTCTTCCCA GCTCTCCTCT 
CCGCCCACCT CCCCACAGAG CTCCCCACGG AAAGGCTACA CTTTGGCTCC GAGTGGCACC 
GTGGATAACT TCTCAGATTC CGGCCACAGT GAAATCTCCT CACGATCCAG TATCGTCAGC 
AACTCCTCCT TCGATTCATT GCCCGTCTCG CTGCCTGACG AGCGGCGCCA GAGGCCGTCA 
GTCAGCATCG TGGAGACCAG CCTGGCCTCG GGCCGGCTGG AGAGGCGGCC GGCGGTGGAG 
CCTGACCAGT ATAGCCTGGG TTCCTGTGCA CCACTGTCTG AGAGCAGGGG CCTATATGCT 
GCAGCCACCG TGATCTCTTC CCCCAGCACG GAGGAGCTTT CCCAGGACCA GGGGGACCGA 
GCCTCCCTGG ACGCTGCTGA CAGTGGCCGG GGGAGCTGGA CGTCGTGCTC GAGCGGCTCC 
CATGATAACA TCCAGACCAT CCAGCACCAG AGGAGCTGGG AGACGCTCCC CTTTGGGCAC 
ACTCACTTTG ATTACCCAGG GGATGCTGCA GGGCTGTGGG CCTCGAGCAG CCATATGGAC 
CAAATCATGT TTCCCGACCA CAGTGCAAAG TACAGCAGGC AGAGTCAGAG CCGAGAGAGT 
CTCGATCAGG CCCAGTCCCG GGCCAGCTGG GCCTCGTCCA CAGGGTACTG GGGAGAGGAC 
TCGGAAGGGG ACACGGGCAC CATCAAGAGA AGGGGGGGTA AGGACGTTTC CCTTGACGCT 
GACAGCAGCA GCATGACAGC CGTGACCGCA GAGGAAGCCA AACCTGCCGC CATGGCTGCC 
CACATAGCCG TGACACCCAG TGCTGCCAAG GGGCTCATCG CCAGGAAGGA GGGCCGCTAC 
CGGGAGCCGC CGCCCACTCC ACCAGGCTAC GTGGGCATCC CCATCACTGA CTTCCCCGAG 
GCCCACCCGC ACCCGGCCCG GAAACCGCCG GACTACACTG TGGCGCTGCA GCGGTCCAGG 
ATGCTGGCCC GGCCGGCCGA ACCCCCTGCC CCAGGCAGCG CCAGGCCTGC CCCCAGGCCG 
CAGTGGCACC GGCCTGGTGA TGGTGACCCA CGCGCCGGCC CCTGTGCACC CCCGGGCCTC 
ACCGCCGAGG AGGACGAAGA TGAACAAGTG TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.
Science signaling6(281)ra51(2013 Jun)
Emery AC,Eiden MV,Mustafa T,Eiden LE

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Identification of a novel beta-catenin-interacting protein.
Biochemical and biophysical research communications273(2)712-7(2000 Jul)
Kawajiri A,Itoh N,Fukata M,Nakagawa M,Yamaga M,Iwamatsu A,Kaibuchi K

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RAPGEF2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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