RAPGEF2 cDNA ORF clone, Gallus gallus(chicken)

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa34950 XM_015285327.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa34951 XM_015285328.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa34942 XM_015285321.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34943 XM_004940909.3
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34944 XM_015285322.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34945 XM_015285323.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34946 XM_015285324.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34947 XM_420387.6
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34948 XM_015285325.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34949 XM_015285326.2
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa07743 XM_004940910.3
Latest version!
Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OGa34947 XM_420387.5 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34950 XM_015285327.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa07743 XM_004940910.2 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa34943 XM_004940909.2 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34951 XM_015285328.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa34944 XM_015285322.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34942 XM_015285321.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34948 XM_015285325.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34946 XM_015285324.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34945 XM_015285323.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa34949 XM_015285326.1 Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa34950
    Clone ID Related Accession (Same CDS sequence) XM_015285327.2 , XM_015285327.1
    Accession Version XM_015285327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AAGGATATCT TTGGTGACAT TGGAATTGGC
    CAGTCTCAGG ATGACAGCAT TGTAGGACTG AGGCAAACAA AGCACATTCC TCCTGCTTTA
    CCTGTCAGTG GAACCCTGTC ATCCAGTAAT CCTGATCTAC TGCAGTCTCA TCACCGTATC
    TTGGACTTCA ATACAACTCC AGACTTACCA GATCAAGTTC TGAGGGTTTT CAAGGCAGAT
    CAGCAAAGTC GCTACATCAT GATCAGTAAG GACACAACAG CAAAAGAGGT GGTCATCCAG
    GCCATCAGGG AATTTGCTCT CACTGCAACT CCTGATGCGT ATTCACTGTG TGAAGTCTCT
    GTCACACCTG AGGGTGTAAT TAAACAAAGG AGGCTTCCTG ATCAGTTATC CAAGCTTGCT
    GACAGGATAC AGCTAAGTGG CAGGTATTAC CTGAAGAACA ACATGGAAAC AGAAACTCTT
    TGTTCAGATG AAGATGCTCA GGAGTTACTA AGAGAGAGTC AAATTTCCCT ACTACAGCTC
    AGTACCGTTG AAGTGGCTAC CCAGCTCTCC ATGAGAAATT TTGAGCTATT CCGTAATATT
    GAACCCACAG AATACATAGA TGACTTGTTT AAACTCAAAT CAAAAACAGG CTGCACTAAT
    CTAAAGAGGT TCGAAGAAGT GATAAATCAA GAAACATTCT GGGTAGCTTC GGAGATTCTA
    AGAGAAACCA ACCAGTTGAA AAGGATGAAA ATCATCAAGC ATTTCATTAA GATAGCGCTA
    CACTGCAGAG AATGCAAGAA TTTCAACTCA ATGTTTGCTA TCATTAGTGG CTTGAATTTG
    GCACCGGTTG CAAGGCTCCG AACTACTTGG GAAAAACTTC CAAGCAAGTA TGAAAAGCTG
    TTCCAAGATC TACAGGATTT GTTTGATCCA TCTAGGAATA TGGCAAAATA CCGTAATGTC
    CTTAACAGCC AAAATCTGCA GCCCCCTATT ATTCCTCTGT TTCCTGTTAT CAAAAAAGAC
    CTTACATTCC TTCATGAAGG AAACGATTCC AAAGTGGAGG GCTTAGTCAA CTTTGAGAAG
    CTGAGAATGA TTGCAAAGGA AATTCGCCAC GTTGGTCGCA TGGCATCTGT CAACATGGAT
    CCTGCTTTAA TGTTTAGAAC TAGGAAGAAG AAATGGAGGA GTTTGGGGTC TCTCAGCCAG
    GGCAGTACAA ATGCAGCTGT GCTTGATGTT GCACAAGCAG GGGGTCATAA AAAGCGGGTC
    CGTCGCAGCT CCTTCCTTAA CGCTAAAAAA CTTTATGAAG ATGCTCAGAT GGCACGGAAA
    GTAAAGCAGT ATCTCTCAAA CTTGGATCTG GAAATGGATG AAGAAAGCCT CCAGACTCTG
    TCTCTACAGT GTGAGCCAGC CACCAACACA TTGCCAAAGA ATACAGGAGA CAAGCGATCT
    GGAAAATCTG AAACATCACC GGTGGCTCCC CGGGCAGGAA TTCAGCAGAA AGTGCAGCAG
    CAGCAGCAAT ATAAAGTAAA CCAAGCACTG CAGGTCCCTG CTGTATCTCT TTATCCCTCT
    CGCAAGAAAG TGCCCGTCAA AGACCTCCCA CCATTTGGCA TTAACTCCCC ACAAGCTTTA
    AAGAAAATTC TTTCATTATC TGAAGAAGGA AGTCTGGACC GCCACAAGAA ACAATCTGAA
    GACACAGTAT CAAGCGCATC TTCACATCTG TCTTCTCCTC CCACCTCACC ACAGAGTTCT
    CCAAGGAAAG GTTACGCTTT GGCTCCCAGT GGCACAGTGG ATAACTTTTC AGACTCTGGT
    CACAGTGAAA TTTCTTCCAG ATCTAGTATT GTCAGCAATT CCTCTTTTGA CTCCATGCCA
    GCGTCCCTGC ATGATGAGAG GAGACAGAGG CATTCTGTCA GCATTGTGGA GACTAATCTT
    GGCGTGGGAA GAATTGATAG AAGGATCATG ATTGAACCAG ATCAATACAG CTTAGGATCT
    TACGCACCAC TGTCAGAGAA CAGAGGCCTG TACGCTGGAG CTACTGTTCT GTCTTCTCCT
    AGTACAGAAG AACTGTCACA GGATCAGGGG GACAGAGCTT CACTCGATGC AGCTGACAGT
    GGCCGTGGTA GCTGGACTTC TTGTTCAAGT GGTTCTCATG ACAACATTCA AACAATACAG
    CACCAGAGAA GCTGGGAGAC GCTGCCTTTT GGACACGCTC ATTTTGATAA TTCAGGCGAT
    GGGGCAGGAC TGTGGGCCTC AGGCAGTCAT GTGGACCAGC TGATGTTCTC CGATCATGGC
    ACAAAGTACA GCCGGCAAAG TCAAGGCAGG GAGGGCCTCG ATCAAGCACA GTCCAGAGCA
    AGCTGGGCAT CCTCAACGGG CTACTGGGGA GAGGACTCGG AAGGTGACAC AGGTACCATA
    AAGCGAAGGG GTGGGAAGGA TGTTTCTGTT GAAGCTGAAA CCAGCTGCAT AACATCCATA
    CCAGCAGAGG AGGCGAAGCC AGCTCCCATG CCCGCTCACA TACCGGTAAC ATCAAGTACT
    ACAAAGGGGC ATATTGCACG AAAAGAAGGT CGGTATCGGG AACCACCTCC AACTCCTCCT
    GGTTATGTAG GAATACCTAT TGCTGAGTTT GCAGAAGGTG TTTCCCATCC AACTAGAAAG
    CCTCCGGATT ACAACGTAGC ACTTCAGAGG TCTAGAATGG TGGCACGGAC GTGTGAGACC
    CAGGGGACGT CGGCTCAACA GCAACAGTCA CATGGCCATT CAACTAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC AAACGAGTCA GATCCACGTC TTGCTCACTA TCAGTCTCAA
    GGGTTTCCCA CAGAGGAAGA TGAAGATGAA CAAGTCTCTG CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140813.1
    CDS517..5004
    Translation

    Target ORF information:

    RefSeq Version XM_015285327.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AAGGATATCT TTGGTGACAT TGGAATTGGC
    CAGTCTCAGG ATGACAGCAT TGTAGGACTG AGGCAAACAA AGCACATTCC TCCTGCTTTA
    CCTGTCAGTG GAACCCTGTC ATCCAGTAAT CCTGATCTAC TGCAGTCTCA TCACCGTATC
    TTGGACTTCA ATACAACTCC AGACTTACCA GATCAAGTTC TGAGGGTTTT CAAGGCAGAT
    CAGCAAAGTC GCTACATCAT GATCAGTAAG GACACAACAG CAAAAGAGGT GGTCATCCAG
    GCCATCAGGG AATTTGCTCT CACTGCAACT CCTGATGCGT ATTCACTGTG TGAAGTCTCT
    GTCACACCTG AGGGTGTAAT TAAACAAAGG AGGCTTCCTG ATCAGTTATC CAAGCTTGCT
    GACAGGATAC AGCTAAGTGG CAGGTATTAC CTGAAGAACA ACATGGAAAC AGAAACTCTT
    TGTTCAGATG AAGATGCTCA GGAGTTACTA AGAGAGAGTC AAATTTCCCT ACTACAGCTC
    AGTACCGTTG AAGTGGCTAC CCAGCTCTCC ATGAGAAATT TTGAGCTATT CCGTAATATT
    GAACCCACAG AATACATAGA TGACTTGTTT AAACTCAAAT CAAAAACAGG CTGCACTAAT
    CTAAAGAGGT TCGAAGAAGT GATAAATCAA GAAACATTCT GGGTAGCTTC GGAGATTCTA
    AGAGAAACCA ACCAGTTGAA AAGGATGAAA ATCATCAAGC ATTTCATTAA GATAGCGCTA
    CACTGCAGAG AATGCAAGAA TTTCAACTCA ATGTTTGCTA TCATTAGTGG CTTGAATTTG
    GCACCGGTTG CAAGGCTCCG AACTACTTGG GAAAAACTTC CAAGCAAGTA TGAAAAGCTG
    TTCCAAGATC TACAGGATTT GTTTGATCCA TCTAGGAATA TGGCAAAATA CCGTAATGTC
    CTTAACAGCC AAAATCTGCA GCCCCCTATT ATTCCTCTGT TTCCTGTTAT CAAAAAAGAC
    CTTACATTCC TTCATGAAGG AAACGATTCC AAAGTGGAGG GCTTAGTCAA CTTTGAGAAG
    CTGAGAATGA TTGCAAAGGA AATTCGCCAC GTTGGTCGCA TGGCATCTGT CAACATGGAT
    CCTGCTTTAA TGTTTAGAAC TAGGAAGAAG AAATGGAGGA GTTTGGGGTC TCTCAGCCAG
    GGCAGTACAA ATGCAGCTGT GCTTGATGTT GCACAAGCAG GGGGTCATAA AAAGCGGGTC
    CGTCGCAGCT CCTTCCTTAA CGCTAAAAAA CTTTATGAAG ATGCTCAGAT GGCACGGAAA
    GTAAAGCAGT ATCTCTCAAA CTTGGATCTG GAAATGGATG AAGAAAGCCT CCAGACTCTG
    TCTCTACAGT GTGAGCCAGC CACCAACACA TTGCCAAAGA ATACAGGAGA CAAGCGATCT
    GGAAAATCTG AAACATCACC GGTGGCTCCC CGGGCAGGAA TTCAGCAGAA AGTGCAGCAG
    CAGCAGCAAT ATAAAGTAAA CCAAGCACTG CAGGTCCCTG CTGTATCTCT TTATCCCTCT
    CGCAAGAAAG TGCCCGTCAA AGACCTCCCA CCATTTGGCA TTAACTCCCC ACAAGCTTTA
    AAGAAAATTC TTTCATTATC TGAAGAAGGA AGTCTGGACC GCCACAAGAA ACAATCTGAA
    GACACAGTAT CAAGCGCATC TTCACATCTG TCTTCTCCTC CCACCTCACC ACAGAGTTCT
    CCAAGGAAAG GTTACGCTTT GGCTCCCAGT GGCACAGTGG ATAACTTTTC AGACTCTGGT
    CACAGTGAAA TTTCTTCCAG ATCTAGTATT GTCAGCAATT CCTCTTTTGA CTCCATGCCA
    GCGTCCCTGC ATGATGAGAG GAGACAGAGG CATTCTGTCA GCATTGTGGA GACTAATCTT
    GGCGTGGGAA GAATTGATAG AAGGATCATG ATTGAACCAG ATCAATACAG CTTAGGATCT
    TACGCACCAC TGTCAGAGAA CAGAGGCCTG TACGCTGGAG CTACTGTTCT GTCTTCTCCT
    AGTACAGAAG AACTGTCACA GGATCAGGGG GACAGAGCTT CACTCGATGC AGCTGACAGT
    GGCCGTGGTA GCTGGACTTC TTGTTCAAGT GGTTCTCATG ACAACATTCA AACAATACAG
    CACCAGAGAA GCTGGGAGAC GCTGCCTTTT GGACACGCTC ATTTTGATAA TTCAGGCGAT
    GGGGCAGGAC TGTGGGCCTC AGGCAGTCAT GTGGACCAGC TGATGTTCTC CGATCATGGC
    ACAAAGTACA GCCGGCAAAG TCAAGGCAGG GAGGGCCTCG ATCAAGCACA GTCCAGAGCA
    AGCTGGGCAT CCTCAACGGG CTACTGGGGA GAGGACTCGG AAGGTGACAC AGGTACCATA
    AAGCGAAGGG GTGGGAAGGA TGTTTCTGTT GAAGCTGAAA CCAGCTGCAT AACATCCATA
    CCAGCAGAGG AGGCGAAGCC AGCTCCCATG CCCGCTCACA TACCGGTAAC ATCAAGTACT
    ACAAAGGGGC ATATTGCACG AAAAGAAGGT CGGTATCGGG AACCACCTCC AACTCCTCCT
    GGTTATGTAG GAATACCTAT TGCTGAGTTT GCAGAAGGTG TTTCCCATCC AACTAGAAAG
    CCTCCGGATT ACAACGTAGC ACTTCAGAGG TCTAGAATGG TGGCACGGAC GTGTGAGACC
    CAGGGGACGT CGGCTCAACA GCAACAGTCA CATGGCCATT CAACTAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC AAACGAGTCA GATCCACGTC TTGCTCACTA TCAGTCTCAA
    GGGTTTCCCA CAGAGGAAGA TGAAGATGAA CAAGTCTCTG CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34950
    Clone ID Related Accession (Same CDS sequence) XM_015285327.2 , XM_015285327.1
    Accession Version XM_015285327.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AAGGATATCT TTGGTGACAT TGGAATTGGC
    CAGTCTCAGG ATGACAGCAT TGTAGGACTG AGGCAAACAA AGCACATTCC TCCTGCTTTA
    CCTGTCAGTG GAACCCTGTC ATCCAGTAAT CCTGATCTAC TGCAGTCTCA TCACCGTATC
    TTGGACTTCA ATACAACTCC AGACTTACCA GATCAAGTTC TGAGGGTTTT CAAGGCAGAT
    CAGCAAAGTC GCTACATCAT GATCAGTAAG GACACAACAG CAAAAGAGGT GGTCATCCAG
    GCCATCAGGG AATTTGCTCT CACTGCAACT CCTGATGCGT ATTCACTGTG TGAAGTCTCT
    GTCACACCTG AGGGTGTAAT TAAACAAAGG AGGCTTCCTG ATCAGTTATC CAAGCTTGCT
    GACAGGATAC AGCTAAGTGG CAGGTATTAC CTGAAGAACA ACATGGAAAC AGAAACTCTT
    TGTTCAGATG AAGATGCTCA GGAGTTACTA AGAGAGAGTC AAATTTCCCT ACTACAGCTC
    AGTACCGTTG AAGTGGCTAC CCAGCTCTCC ATGAGAAATT TTGAGCTATT CCGTAATATT
    GAACCCACAG AATACATAGA TGACTTGTTT AAACTCAAAT CAAAAACAGG CTGCACTAAT
    CTAAAGAGGT TCGAAGAAGT GATAAATCAA GAAACATTCT GGGTAGCTTC GGAGATTCTA
    AGAGAAACCA ACCAGTTGAA AAGGATGAAA ATCATCAAGC ATTTCATTAA GATAGCGCTA
    CACTGCAGAG AATGCAAGAA TTTCAACTCA ATGTTTGCTA TCATTAGTGG CTTGAATTTG
    GCACCGGTTG CAAGGCTCCG AACTACTTGG GAAAAACTTC CAAGCAAGTA TGAAAAGCTG
    TTCCAAGATC TACAGGATTT GTTTGATCCA TCTAGGAATA TGGCAAAATA CCGTAATGTC
    CTTAACAGCC AAAATCTGCA GCCCCCTATT ATTCCTCTGT TTCCTGTTAT CAAAAAAGAC
    CTTACATTCC TTCATGAAGG AAACGATTCC AAAGTGGAGG GCTTAGTCAA CTTTGAGAAG
    CTGAGAATGA TTGCAAAGGA AATTCGCCAC GTTGGTCGCA TGGCATCTGT CAACATGGAT
    CCTGCTTTAA TGTTTAGAAC TAGGAAGAAG AAATGGAGGA GTTTGGGGTC TCTCAGCCAG
    GGCAGTACAA ATGCAGCTGT GCTTGATGTT GCACAAGCAG GGGGTCATAA AAAGCGGGTC
    CGTCGCAGCT CCTTCCTTAA CGCTAAAAAA CTTTATGAAG ATGCTCAGAT GGCACGGAAA
    GTAAAGCAGT ATCTCTCAAA CTTGGATCTG GAAATGGATG AAGAAAGCCT CCAGACTCTG
    TCTCTACAGT GTGAGCCAGC CACCAACACA TTGCCAAAGA ATACAGGAGA CAAGCGATCT
    GGAAAATCTG AAACATCACC GGTGGCTCCC CGGGCAGGAA TTCAGCAGAA AGTGCAGCAG
    CAGCAGCAAT ATAAAGTAAA CCAAGCACTG CAGGTCCCTG CTGTATCTCT TTATCCCTCT
    CGCAAGAAAG TGCCCGTCAA AGACCTCCCA CCATTTGGCA TTAACTCCCC ACAAGCTTTA
    AAGAAAATTC TTTCATTATC TGAAGAAGGA AGTCTGGACC GCCACAAGAA ACAATCTGAA
    GACACAGTAT CAAGCGCATC TTCACATCTG TCTTCTCCTC CCACCTCACC ACAGAGTTCT
    CCAAGGAAAG GTTACGCTTT GGCTCCCAGT GGCACAGTGG ATAACTTTTC AGACTCTGGT
    CACAGTGAAA TTTCTTCCAG ATCTAGTATT GTCAGCAATT CCTCTTTTGA CTCCATGCCA
    GCGTCCCTGC ATGATGAGAG GAGACAGAGG CATTCTGTCA GCATTGTGGA GACTAATCTT
    GGCGTGGGAA GAATTGATAG AAGGATCATG ATTGAACCAG ATCAATACAG CTTAGGATCT
    TACGCACCAC TGTCAGAGAA CAGAGGCCTG TACGCTGGAG CTACTGTTCT GTCTTCTCCT
    AGTACAGAAG AACTGTCACA GGATCAGGGG GACAGAGCTT CACTCGATGC AGCTGACAGT
    GGCCGTGGTA GCTGGACTTC TTGTTCAAGT GGTTCTCATG ACAACATTCA AACAATACAG
    CACCAGAGAA GCTGGGAGAC GCTGCCTTTT GGACACGCTC ATTTTGATAA TTCAGGCGAT
    GGGGCAGGAC TGTGGGCCTC AGGCAGTCAT GTGGACCAGC TGATGTTCTC CGATCATGGC
    ACAAAGTACA GCCGGCAAAG TCAAGGCAGG GAGGGCCTCG ATCAAGCACA GTCCAGAGCA
    AGCTGGGCAT CCTCAACGGG CTACTGGGGA GAGGACTCGG AAGGTGACAC AGGTACCATA
    AAGCGAAGGG GTGGGAAGGA TGTTTCTGTT GAAGCTGAAA CCAGCTGCAT AACATCCATA
    CCAGCAGAGG AGGCGAAGCC AGCTCCCATG CCCGCTCACA TACCGGTAAC ATCAAGTACT
    ACAAAGGGGC ATATTGCACG AAAAGAAGGT CGGTATCGGG AACCACCTCC AACTCCTCCT
    GGTTATGTAG GAATACCTAT TGCTGAGTTT GCAGAAGGTG TTTCCCATCC AACTAGAAAG
    CCTCCGGATT ACAACGTAGC ACTTCAGAGG TCTAGAATGG TGGCACGGAC GTGTGAGACC
    CAGGGGACGT CGGCTCAACA GCAACAGTCA CATGGCCATT CAACTAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC AAACGAGTCA GATCCACGTC TTGCTCACTA TCAGTCTCAA
    GGGTTTCCCA CAGAGGAAGA TGAAGATGAA CAAGTCTCTG CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140813.1
    CDS518..5005
    Translation

    Target ORF information:

    RefSeq Version XM_015285327.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AAGGATATCT TTGGTGACAT TGGAATTGGC
    CAGTCTCAGG ATGACAGCAT TGTAGGACTG AGGCAAACAA AGCACATTCC TCCTGCTTTA
    CCTGTCAGTG GAACCCTGTC ATCCAGTAAT CCTGATCTAC TGCAGTCTCA TCACCGTATC
    TTGGACTTCA ATACAACTCC AGACTTACCA GATCAAGTTC TGAGGGTTTT CAAGGCAGAT
    CAGCAAAGTC GCTACATCAT GATCAGTAAG GACACAACAG CAAAAGAGGT GGTCATCCAG
    GCCATCAGGG AATTTGCTCT CACTGCAACT CCTGATGCGT ATTCACTGTG TGAAGTCTCT
    GTCACACCTG AGGGTGTAAT TAAACAAAGG AGGCTTCCTG ATCAGTTATC CAAGCTTGCT
    GACAGGATAC AGCTAAGTGG CAGGTATTAC CTGAAGAACA ACATGGAAAC AGAAACTCTT
    TGTTCAGATG AAGATGCTCA GGAGTTACTA AGAGAGAGTC AAATTTCCCT ACTACAGCTC
    AGTACCGTTG AAGTGGCTAC CCAGCTCTCC ATGAGAAATT TTGAGCTATT CCGTAATATT
    GAACCCACAG AATACATAGA TGACTTGTTT AAACTCAAAT CAAAAACAGG CTGCACTAAT
    CTAAAGAGGT TCGAAGAAGT GATAAATCAA GAAACATTCT GGGTAGCTTC GGAGATTCTA
    AGAGAAACCA ACCAGTTGAA AAGGATGAAA ATCATCAAGC ATTTCATTAA GATAGCGCTA
    CACTGCAGAG AATGCAAGAA TTTCAACTCA ATGTTTGCTA TCATTAGTGG CTTGAATTTG
    GCACCGGTTG CAAGGCTCCG AACTACTTGG GAAAAACTTC CAAGCAAGTA TGAAAAGCTG
    TTCCAAGATC TACAGGATTT GTTTGATCCA TCTAGGAATA TGGCAAAATA CCGTAATGTC
    CTTAACAGCC AAAATCTGCA GCCCCCTATT ATTCCTCTGT TTCCTGTTAT CAAAAAAGAC
    CTTACATTCC TTCATGAAGG AAACGATTCC AAAGTGGAGG GCTTAGTCAA CTTTGAGAAG
    CTGAGAATGA TTGCAAAGGA AATTCGCCAC GTTGGTCGCA TGGCATCTGT CAACATGGAT
    CCTGCTTTAA TGTTTAGAAC TAGGAAGAAG AAATGGAGGA GTTTGGGGTC TCTCAGCCAG
    GGCAGTACAA ATGCAGCTGT GCTTGATGTT GCACAAGCAG GGGGTCATAA AAAGCGGGTC
    CGTCGCAGCT CCTTCCTTAA CGCTAAAAAA CTTTATGAAG ATGCTCAGAT GGCACGGAAA
    GTAAAGCAGT ATCTCTCAAA CTTGGATCTG GAAATGGATG AAGAAAGCCT CCAGACTCTG
    TCTCTACAGT GTGAGCCAGC CACCAACACA TTGCCAAAGA ATACAGGAGA CAAGCGATCT
    GGAAAATCTG AAACATCACC GGTGGCTCCC CGGGCAGGAA TTCAGCAGAA AGTGCAGCAG
    CAGCAGCAAT ATAAAGTAAA CCAAGCACTG CAGGTCCCTG CTGTATCTCT TTATCCCTCT
    CGCAAGAAAG TGCCCGTCAA AGACCTCCCA CCATTTGGCA TTAACTCCCC ACAAGCTTTA
    AAGAAAATTC TTTCATTATC TGAAGAAGGA AGTCTGGACC GCCACAAGAA ACAATCTGAA
    GACACAGTAT CAAGCGCATC TTCACATCTG TCTTCTCCTC CCACCTCACC ACAGAGTTCT
    CCAAGGAAAG GTTACGCTTT GGCTCCCAGT GGCACAGTGG ATAACTTTTC AGACTCTGGT
    CACAGTGAAA TTTCTTCCAG ATCTAGTATT GTCAGCAATT CCTCTTTTGA CTCCATGCCA
    GCGTCCCTGC ATGATGAGAG GAGACAGAGG CATTCTGTCA GCATTGTGGA GACTAATCTT
    GGCGTGGGAA GAATTGATAG AAGGATCATG ATTGAACCAG ATCAATACAG CTTAGGATCT
    TACGCACCAC TGTCAGAGAA CAGAGGCCTG TACGCTGGAG CTACTGTTCT GTCTTCTCCT
    AGTACAGAAG AACTGTCACA GGATCAGGGG GACAGAGCTT CACTCGATGC AGCTGACAGT
    GGCCGTGGTA GCTGGACTTC TTGTTCAAGT GGTTCTCATG ACAACATTCA AACAATACAG
    CACCAGAGAA GCTGGGAGAC GCTGCCTTTT GGACACGCTC ATTTTGATAA TTCAGGCGAT
    GGGGCAGGAC TGTGGGCCTC AGGCAGTCAT GTGGACCAGC TGATGTTCTC CGATCATGGC
    ACAAAGTACA GCCGGCAAAG TCAAGGCAGG GAGGGCCTCG ATCAAGCACA GTCCAGAGCA
    AGCTGGGCAT CCTCAACGGG CTACTGGGGA GAGGACTCGG AAGGTGACAC AGGTACCATA
    AAGCGAAGGG GTGGGAAGGA TGTTTCTGTT GAAGCTGAAA CCAGCTGCAT AACATCCATA
    CCAGCAGAGG AGGCGAAGCC AGCTCCCATG CCCGCTCACA TACCGGTAAC ATCAAGTACT
    ACAAAGGGGC ATATTGCACG AAAAGAAGGT CGGTATCGGG AACCACCTCC AACTCCTCCT
    GGTTATGTAG GAATACCTAT TGCTGAGTTT GCAGAAGGTG TTTCCCATCC AACTAGAAAG
    CCTCCGGATT ACAACGTAGC ACTTCAGAGG TCTAGAATGG TGGCACGGAC GTGTGAGACC
    CAGGGGACGT CGGCTCAACA GCAACAGTCA CATGGCCATT CAACTAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC AAACGAGTCA GATCCACGTC TTGCTCACTA TCAGTCTCAA
    GGGTTTCCCA CAGAGGAAGA TGAAGATGAA CAAGTCTCTG CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34951
    Clone ID Related Accession (Same CDS sequence) XM_015285328.2 , XM_015285328.1
    Accession Version XM_015285328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
    GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA
    TCCAGTCACT CAGGATGTAG CATCACAAGT GATTCGGGAA GCAGCAGCCT TTCTGATATC
    TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA
    GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG
    AGTAGAGATA TTGTCAGAGA TTGCTTGGAG AAAGATCCAA TAGACAGGAC GGATGATGAC
    ATTGAACAAC TACTGGAATT CATGCATCAG TTGCCTGCTT TTGCCAACAT GACAATGTCA
    GTGAGGAGAG AGCTTTGTGC TGTAATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT
    GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTCTGAATGG CTCTGTGGAA
    GTGACATATC CTGATGGAAG AACAGAGATA CTGTGCATGG GAAACAGTTT TGGAGTTTCC
    CCTACCATGG AAAAGGAATA TATGAAGGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTTTGTA TAGCTCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAAAACATG
    CAGAAGGTAG AAGAGGAAGG AGAGATTGTG ATGGTTAAGG AGCACAGAGA GCTTGATCGC
    ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CGGAAAGGTT AACAATGCAT
    TTGGTGGAAG AACACTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACGTAT
    CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGGAAAAAGT TGTTGGAGTG GTTCAATGAC
    CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT
    GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG
    AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGCGCCGC TAAAGCAAAA
    AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG
    GGAGGGTCAG AAAAGGGATT TGGGATCTTT GTTGATAGCG TAGATTTTGG TAGTAAAGCT
    ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATATTGGAAG TGAATGGTCA AAACTTCGAA
    AATGTTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT
    GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTTTTAACAC GGTTGTCAGA GGAAAAACGA
    AATGGTGCTC CTCACTTGCC TAAAATTGGT GATATTAAAA AAGCGAGCCG TTATTCCATC
    CCAGATCTGG CTGTTGATGT GGAACAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT
    AAAGCCAACA CAGTTGGGGG AAGAAATAAG TTAAAGAAGA TACTTGACAA GACTCGAATC
    AGCATTCTGC CTCAGAAACC ATACAAGGAT ATCTTTGGTG ACATTGGAAT TGGCCAGTCT
    CAGGATGACA GCATTGTAGG ACTGAGGCAA ACAAAGCACA TTCCTCCTGC TTTACCTGTC
    AGTGGAACCC TGTCATCCAG TAATCCTGAT CTACTGCAGT CTCATCACCG TATCTTGGAC
    TTCAATACAA CTCCAGACTT ACCAGATCAA GTTCTGAGGG TTTTCAAGGC AGATCAGCAA
    AGTCGCTACA TCATGATCAG TAAGGACACA ACAGCAAAAG AGGTGGTCAT CCAGGCCATC
    AGGGAATTTG CTCTCACTGC AACTCCTGAT GCGTATTCAC TGTGTGAAGT CTCTGTCACA
    CCTGAGGGTG TAATTAAACA AAGGAGGCTT CCTGATCAGT TATCCAAGCT TGCTGACAGG
    ATACAGCTAA GTGGCAGGTA TTACCTGAAG AACAACATGG AAACAGAAAC TCTTTGTTCA
    GATGAAGATG CTCAGGAGTT ACTAAGAGAG AGTCAAATTT CCCTACTACA GCTCAGTACC
    GTTGAAGTGG CTACCCAGCT CTCCATGAGA AATTTTGAGC TATTCCGTAA TATTGAACCC
    ACAGAATACA TAGATGACTT GTTTAAACTC AAATCAAAAA CAGGCTGCAC TAATCTAAAG
    AGGTTCGAAG AAGTGATAAA TCAAGAAACA TTCTGGGTAG CTTCGGAGAT TCTAAGAGAA
    ACCAACCAGT TGAAAAGGAT GAAAATCATC AAGCATTTCA TTAAGATAGC GCTACACTGC
    AGAGAATGCA AGAATTTCAA CTCAATGTTT GCTATCATTA GTGGCTTGAA TTTGGCACCG
    GTTGCAAGGC TCCGAACTAC TTGGGAAAAA CTTCCAAGCA AGTATGAAAA GCTGTTCCAA
    GATCTACAGG ATTTGTTTGA TCCATCTAGG AATATGGCAA AATACCGTAA TGTCCTTAAC
    AGCCAAAATC TGCAGCCCCC TATTATTCCT CTGTTTCCTG TTATCAAAAA AGACCTTACA
    TTCCTTCATG AAGGAAACGA TTCCAAAGTG GAGGGCTTAG TCAACTTTGA GAAGCTGAGA
    ATGATTGCAA AGGAAATTCG CCACGTTGGT CGCATGGCAT CTGTCAACAT GGATCCTGCT
    TTAATGTTTA GAACTAGGAA GAAGAAATGG AGGAGTTTGG GGTCTCTCAG CCAGGGCAGT
    ACAAATGCAG CTGTGCTTGA TGTTGCACAA GCAGGGGGTC ATAAAAAGCG GGTCCGTCGC
    AGCTCCTTCC TTAACGCTAA AAAACTTTAT GAAGATGCTC AGATGGCACG GAAAGTAAAG
    CAGTATCTCT CAAACTTGGA TCTGGAAATG GATGAAGAAA GCCTCCAGAC TCTGTCTCTA
    CAGTGTGAGC CAGCCACCAA CACATTGCCA AAGAATACAG GAGACAAGCG ATCTGGAAAA
    TCTGAAACAT CACCGGTGGC TCCCCGGGCA GGAATTCAGC AGAAAGTGCA GCAGCAGCAG
    CAATATAAAG TAAACCAAGC ACTGCAGGTC CCTGCTGTAT CTCTTTATCC CTCTCGCAAG
    AAAGTGCCCG TCAAAGACCT CCCACCATTT GGCATTAACT CCCCACAAGC TTTAAAGAAA
    ATTCTTTCAT TATCTGAAGA AGGAAGTCTG GACCGCCACA AGAAACAATC TGAAGACACA
    GTATCAAGCG CATCTTCACA TCTGTCTTCT CCTCCCACCT CACCACAGAG TTCTCCAAGG
    AAAGGTTACG CTTTGGCTCC CAGTGGCACA GTGGATAACT TTTCAGACTC TGGTCACAGT
    GAAATTTCTT CCAGATCTAG TATTGTCAGC AATTCCTCTT TTGACTCCAT GCCAGCGTCC
    CTGCATGATG AGAGGAGACA GAGGCATTCT GTCAGCATTG TGGAGACTAA TCTTGGCGTG
    GGAAGAATTG ATAGAAGGAT CATGATTGAA CCAGATCAAT ACAGCTTAGG ATCTTACGCA
    CCACTGTCAG AGAACAGAGG CCTGTACGCT GGAGCTACTG TTCTGTCTTC TCCTAGTACA
    GAAGAACTGT CACAGGATCA GGGGGACAGA GCTTCACTCG ATGCAGCTGA CAGTGGCCGT
    GGTAGCTGGA CTTCTTGTTC AAGTGGTTCT CATGACAACA TTCAAACAAT ACAGCACCAG
    AGAAGCTGGG AGACGCTGCC TTTTGGACAC GCTCATTTTG ATAATTCAGG CGATGGGGCA
    GGACTGTGGG CCTCAGGCAG TCATGTGGAC CAGCTGATGT TCTCCGATCA TGGCACAAAG
    TACAGCCGGC AAAGTCAAGG CAGGGAGGGC CTCGATCAAG CACAGTCCAG AGCAAGCTGG
    GCATCCTCAA CGGGCTACTG GGGAGAGGAC TCGGAAGGTG ACACAGGTAC CATAAAGCGA
    AGGGGTGGGA AGGATGTTTC TGTTGAAGCT GAAACCAGCT GCATAACATC CATACCAGCA
    GAGGAGGCGA AGCCAGCTCC CATGCCCGCT CACATACCGG TAACATCAAG TACTACAAAG
    GGGCATATTG CACGAAAAGA AGGTCGGTAT CGGGAACCAC CTCCAACTCC TCCTGGTTAT
    GTAGGAATAC CTATTGCTGA GTTTGCAGAA GGTGTTTCCC ATCCAACTAG AAAGCCTCCG
    GATTACAACG TAGCACTTCA GAGGTCTAGA ATGGTGGCAC GGACGTGTGA GACCCAGGGG
    ACGTCGGCTC AACAGCAACA GTCACATGGC CATTCAACTA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCAAACGA GTCAGATCCA CGTCTTGCTC ACTATCAGTC TCAAGGGTTT
    CCCACAGAGG AAGATGAAGA TGAACAAGTC TCTGCTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140814.1
    CDS16..4497
    Translation

    Target ORF information:

    RefSeq Version XM_015285328.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
    GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA
    TCCAGTCACT CAGGATGTAG CATCACAAGT GATTCGGGAA GCAGCAGCCT TTCTGATATC
    TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA
    GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG
    AGTAGAGATA TTGTCAGAGA TTGCTTGGAG AAAGATCCAA TAGACAGGAC GGATGATGAC
    ATTGAACAAC TACTGGAATT CATGCATCAG TTGCCTGCTT TTGCCAACAT GACAATGTCA
    GTGAGGAGAG AGCTTTGTGC TGTAATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT
    GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTCTGAATGG CTCTGTGGAA
    GTGACATATC CTGATGGAAG AACAGAGATA CTGTGCATGG GAAACAGTTT TGGAGTTTCC
    CCTACCATGG AAAAGGAATA TATGAAGGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTTTGTA TAGCTCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAAAACATG
    CAGAAGGTAG AAGAGGAAGG AGAGATTGTG ATGGTTAAGG AGCACAGAGA GCTTGATCGC
    ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CGGAAAGGTT AACAATGCAT
    TTGGTGGAAG AACACTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACGTAT
    CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGGAAAAAGT TGTTGGAGTG GTTCAATGAC
    CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT
    GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG
    AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGCGCCGC TAAAGCAAAA
    AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG
    GGAGGGTCAG AAAAGGGATT TGGGATCTTT GTTGATAGCG TAGATTTTGG TAGTAAAGCT
    ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATATTGGAAG TGAATGGTCA AAACTTCGAA
    AATGTTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT
    GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTTTTAACAC GGTTGTCAGA GGAAAAACGA
    AATGGTGCTC CTCACTTGCC TAAAATTGGT GATATTAAAA AAGCGAGCCG TTATTCCATC
    CCAGATCTGG CTGTTGATGT GGAACAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT
    AAAGCCAACA CAGTTGGGGG AAGAAATAAG TTAAAGAAGA TACTTGACAA GACTCGAATC
    AGCATTCTGC CTCAGAAACC ATACAAGGAT ATCTTTGGTG ACATTGGAAT TGGCCAGTCT
    CAGGATGACA GCATTGTAGG ACTGAGGCAA ACAAAGCACA TTCCTCCTGC TTTACCTGTC
    AGTGGAACCC TGTCATCCAG TAATCCTGAT CTACTGCAGT CTCATCACCG TATCTTGGAC
    TTCAATACAA CTCCAGACTT ACCAGATCAA GTTCTGAGGG TTTTCAAGGC AGATCAGCAA
    AGTCGCTACA TCATGATCAG TAAGGACACA ACAGCAAAAG AGGTGGTCAT CCAGGCCATC
    AGGGAATTTG CTCTCACTGC AACTCCTGAT GCGTATTCAC TGTGTGAAGT CTCTGTCACA
    CCTGAGGGTG TAATTAAACA AAGGAGGCTT CCTGATCAGT TATCCAAGCT TGCTGACAGG
    ATACAGCTAA GTGGCAGGTA TTACCTGAAG AACAACATGG AAACAGAAAC TCTTTGTTCA
    GATGAAGATG CTCAGGAGTT ACTAAGAGAG AGTCAAATTT CCCTACTACA GCTCAGTACC
    GTTGAAGTGG CTACCCAGCT CTCCATGAGA AATTTTGAGC TATTCCGTAA TATTGAACCC
    ACAGAATACA TAGATGACTT GTTTAAACTC AAATCAAAAA CAGGCTGCAC TAATCTAAAG
    AGGTTCGAAG AAGTGATAAA TCAAGAAACA TTCTGGGTAG CTTCGGAGAT TCTAAGAGAA
    ACCAACCAGT TGAAAAGGAT GAAAATCATC AAGCATTTCA TTAAGATAGC GCTACACTGC
    AGAGAATGCA AGAATTTCAA CTCAATGTTT GCTATCATTA GTGGCTTGAA TTTGGCACCG
    GTTGCAAGGC TCCGAACTAC TTGGGAAAAA CTTCCAAGCA AGTATGAAAA GCTGTTCCAA
    GATCTACAGG ATTTGTTTGA TCCATCTAGG AATATGGCAA AATACCGTAA TGTCCTTAAC
    AGCCAAAATC TGCAGCCCCC TATTATTCCT CTGTTTCCTG TTATCAAAAA AGACCTTACA
    TTCCTTCATG AAGGAAACGA TTCCAAAGTG GAGGGCTTAG TCAACTTTGA GAAGCTGAGA
    ATGATTGCAA AGGAAATTCG CCACGTTGGT CGCATGGCAT CTGTCAACAT GGATCCTGCT
    TTAATGTTTA GAACTAGGAA GAAGAAATGG AGGAGTTTGG GGTCTCTCAG CCAGGGCAGT
    ACAAATGCAG CTGTGCTTGA TGTTGCACAA GCAGGGGGTC ATAAAAAGCG GGTCCGTCGC
    AGCTCCTTCC TTAACGCTAA AAAACTTTAT GAAGATGCTC AGATGGCACG GAAAGTAAAG
    CAGTATCTCT CAAACTTGGA TCTGGAAATG GATGAAGAAA GCCTCCAGAC TCTGTCTCTA
    CAGTGTGAGC CAGCCACCAA CACATTGCCA AAGAATACAG GAGACAAGCG ATCTGGAAAA
    TCTGAAACAT CACCGGTGGC TCCCCGGGCA GGAATTCAGC AGAAAGTGCA GCAGCAGCAG
    CAATATAAAG TAAACCAAGC ACTGCAGGTC CCTGCTGTAT CTCTTTATCC CTCTCGCAAG
    AAAGTGCCCG TCAAAGACCT CCCACCATTT GGCATTAACT CCCCACAAGC TTTAAAGAAA
    ATTCTTTCAT TATCTGAAGA AGGAAGTCTG GACCGCCACA AGAAACAATC TGAAGACACA
    GTATCAAGCG CATCTTCACA TCTGTCTTCT CCTCCCACCT CACCACAGAG TTCTCCAAGG
    AAAGGTTACG CTTTGGCTCC CAGTGGCACA GTGGATAACT TTTCAGACTC TGGTCACAGT
    GAAATTTCTT CCAGATCTAG TATTGTCAGC AATTCCTCTT TTGACTCCAT GCCAGCGTCC
    CTGCATGATG AGAGGAGACA GAGGCATTCT GTCAGCATTG TGGAGACTAA TCTTGGCGTG
    GGAAGAATTG ATAGAAGGAT CATGATTGAA CCAGATCAAT ACAGCTTAGG ATCTTACGCA
    CCACTGTCAG AGAACAGAGG CCTGTACGCT GGAGCTACTG TTCTGTCTTC TCCTAGTACA
    GAAGAACTGT CACAGGATCA GGGGGACAGA GCTTCACTCG ATGCAGCTGA CAGTGGCCGT
    GGTAGCTGGA CTTCTTGTTC AAGTGGTTCT CATGACAACA TTCAAACAAT ACAGCACCAG
    AGAAGCTGGG AGACGCTGCC TTTTGGACAC GCTCATTTTG ATAATTCAGG CGATGGGGCA
    GGACTGTGGG CCTCAGGCAG TCATGTGGAC CAGCTGATGT TCTCCGATCA TGGCACAAAG
    TACAGCCGGC AAAGTCAAGG CAGGGAGGGC CTCGATCAAG CACAGTCCAG AGCAAGCTGG
    GCATCCTCAA CGGGCTACTG GGGAGAGGAC TCGGAAGGTG ACACAGGTAC CATAAAGCGA
    AGGGGTGGGA AGGATGTTTC TGTTGAAGCT GAAACCAGCT GCATAACATC CATACCAGCA
    GAGGAGGCGA AGCCAGCTCC CATGCCCGCT CACATACCGG TAACATCAAG TACTACAAAG
    GGGCATATTG CACGAAAAGA AGGTCGGTAT CGGGAACCAC CTCCAACTCC TCCTGGTTAT
    GTAGGAATAC CTATTGCTGA GTTTGCAGAA GGTGTTTCCC ATCCAACTAG AAAGCCTCCG
    GATTACAACG TAGCACTTCA GAGGTCTAGA ATGGTGGCAC GGACGTGTGA GACCCAGGGG
    ACGTCGGCTC AACAGCAACA GTCACATGGC CATTCAACTA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCAAACGA GTCAGATCCA CGTCTTGCTC ACTATCAGTC TCAAGGGTTT
    CCCACAGAGG AAGATGAAGA TGAACAAGTC TCTGCTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34951
    Clone ID Related Accession (Same CDS sequence) XM_015285328.2 , XM_015285328.1
    Accession Version XM_015285328.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
    GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA
    TCCAGTCACT CAGGATGTAG CATCACAAGT GATTCGGGAA GCAGCAGCCT TTCTGATATC
    TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA
    GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG
    AGTAGAGATA TTGTCAGAGA TTGCTTGGAG AAAGATCCAA TAGACAGGAC GGATGATGAC
    ATTGAACAAC TACTGGAATT CATGCATCAG TTGCCTGCTT TTGCCAACAT GACAATGTCA
    GTGAGGAGAG AGCTTTGTGC TGTAATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT
    GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTCTGAATGG CTCTGTGGAA
    GTGACATATC CTGATGGAAG AACAGAGATA CTGTGCATGG GAAACAGTTT TGGAGTTTCC
    CCTACCATGG AAAAGGAATA TATGAAGGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTTTGTA TAGCTCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAAAACATG
    CAGAAGGTAG AAGAGGAAGG AGAGATTGTG ATGGTTAAGG AGCACAGAGA GCTTGATCGC
    ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CGGAAAGGTT AACAATGCAT
    TTGGTGGAAG AACACTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACGTAT
    CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGGAAAAAGT TGTTGGAGTG GTTCAATGAC
    CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT
    GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG
    AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGCGCCGC TAAAGCAAAA
    AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG
    GGAGGGTCAG AAAAGGGATT TGGGATCTTT GTTGATAGCG TAGATTTTGG TAGTAAAGCT
    ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATATTGGAAG TGAATGGTCA AAACTTCGAA
    AATGTTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT
    GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTTTTAACAC GGTTGTCAGA GGAAAAACGA
    AATGGTGCTC CTCACTTGCC TAAAATTGGT GATATTAAAA AAGCGAGCCG TTATTCCATC
    CCAGATCTGG CTGTTGATGT GGAACAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT
    AAAGCCAACA CAGTTGGGGG AAGAAATAAG TTAAAGAAGA TACTTGACAA GACTCGAATC
    AGCATTCTGC CTCAGAAACC ATACAAGGAT ATCTTTGGTG ACATTGGAAT TGGCCAGTCT
    CAGGATGACA GCATTGTAGG ACTGAGGCAA ACAAAGCACA TTCCTCCTGC TTTACCTGTC
    AGTGGAACCC TGTCATCCAG TAATCCTGAT CTACTGCAGT CTCATCACCG TATCTTGGAC
    TTCAATACAA CTCCAGACTT ACCAGATCAA GTTCTGAGGG TTTTCAAGGC AGATCAGCAA
    AGTCGCTACA TCATGATCAG TAAGGACACA ACAGCAAAAG AGGTGGTCAT CCAGGCCATC
    AGGGAATTTG CTCTCACTGC AACTCCTGAT GCGTATTCAC TGTGTGAAGT CTCTGTCACA
    CCTGAGGGTG TAATTAAACA AAGGAGGCTT CCTGATCAGT TATCCAAGCT TGCTGACAGG
    ATACAGCTAA GTGGCAGGTA TTACCTGAAG AACAACATGG AAACAGAAAC TCTTTGTTCA
    GATGAAGATG CTCAGGAGTT ACTAAGAGAG AGTCAAATTT CCCTACTACA GCTCAGTACC
    GTTGAAGTGG CTACCCAGCT CTCCATGAGA AATTTTGAGC TATTCCGTAA TATTGAACCC
    ACAGAATACA TAGATGACTT GTTTAAACTC AAATCAAAAA CAGGCTGCAC TAATCTAAAG
    AGGTTCGAAG AAGTGATAAA TCAAGAAACA TTCTGGGTAG CTTCGGAGAT TCTAAGAGAA
    ACCAACCAGT TGAAAAGGAT GAAAATCATC AAGCATTTCA TTAAGATAGC GCTACACTGC
    AGAGAATGCA AGAATTTCAA CTCAATGTTT GCTATCATTA GTGGCTTGAA TTTGGCACCG
    GTTGCAAGGC TCCGAACTAC TTGGGAAAAA CTTCCAAGCA AGTATGAAAA GCTGTTCCAA
    GATCTACAGG ATTTGTTTGA TCCATCTAGG AATATGGCAA AATACCGTAA TGTCCTTAAC
    AGCCAAAATC TGCAGCCCCC TATTATTCCT CTGTTTCCTG TTATCAAAAA AGACCTTACA
    TTCCTTCATG AAGGAAACGA TTCCAAAGTG GAGGGCTTAG TCAACTTTGA GAAGCTGAGA
    ATGATTGCAA AGGAAATTCG CCACGTTGGT CGCATGGCAT CTGTCAACAT GGATCCTGCT
    TTAATGTTTA GAACTAGGAA GAAGAAATGG AGGAGTTTGG GGTCTCTCAG CCAGGGCAGT
    ACAAATGCAG CTGTGCTTGA TGTTGCACAA GCAGGGGGTC ATAAAAAGCG GGTCCGTCGC
    AGCTCCTTCC TTAACGCTAA AAAACTTTAT GAAGATGCTC AGATGGCACG GAAAGTAAAG
    CAGTATCTCT CAAACTTGGA TCTGGAAATG GATGAAGAAA GCCTCCAGAC TCTGTCTCTA
    CAGTGTGAGC CAGCCACCAA CACATTGCCA AAGAATACAG GAGACAAGCG ATCTGGAAAA
    TCTGAAACAT CACCGGTGGC TCCCCGGGCA GGAATTCAGC AGAAAGTGCA GCAGCAGCAG
    CAATATAAAG TAAACCAAGC ACTGCAGGTC CCTGCTGTAT CTCTTTATCC CTCTCGCAAG
    AAAGTGCCCG TCAAAGACCT CCCACCATTT GGCATTAACT CCCCACAAGC TTTAAAGAAA
    ATTCTTTCAT TATCTGAAGA AGGAAGTCTG GACCGCCACA AGAAACAATC TGAAGACACA
    GTATCAAGCG CATCTTCACA TCTGTCTTCT CCTCCCACCT CACCACAGAG TTCTCCAAGG
    AAAGGTTACG CTTTGGCTCC CAGTGGCACA GTGGATAACT TTTCAGACTC TGGTCACAGT
    GAAATTTCTT CCAGATCTAG TATTGTCAGC AATTCCTCTT TTGACTCCAT GCCAGCGTCC
    CTGCATGATG AGAGGAGACA GAGGCATTCT GTCAGCATTG TGGAGACTAA TCTTGGCGTG
    GGAAGAATTG ATAGAAGGAT CATGATTGAA CCAGATCAAT ACAGCTTAGG ATCTTACGCA
    CCACTGTCAG AGAACAGAGG CCTGTACGCT GGAGCTACTG TTCTGTCTTC TCCTAGTACA
    GAAGAACTGT CACAGGATCA GGGGGACAGA GCTTCACTCG ATGCAGCTGA CAGTGGCCGT
    GGTAGCTGGA CTTCTTGTTC AAGTGGTTCT CATGACAACA TTCAAACAAT ACAGCACCAG
    AGAAGCTGGG AGACGCTGCC TTTTGGACAC GCTCATTTTG ATAATTCAGG CGATGGGGCA
    GGACTGTGGG CCTCAGGCAG TCATGTGGAC CAGCTGATGT TCTCCGATCA TGGCACAAAG
    TACAGCCGGC AAAGTCAAGG CAGGGAGGGC CTCGATCAAG CACAGTCCAG AGCAAGCTGG
    GCATCCTCAA CGGGCTACTG GGGAGAGGAC TCGGAAGGTG ACACAGGTAC CATAAAGCGA
    AGGGGTGGGA AGGATGTTTC TGTTGAAGCT GAAACCAGCT GCATAACATC CATACCAGCA
    GAGGAGGCGA AGCCAGCTCC CATGCCCGCT CACATACCGG TAACATCAAG TACTACAAAG
    GGGCATATTG CACGAAAAGA AGGTCGGTAT CGGGAACCAC CTCCAACTCC TCCTGGTTAT
    GTAGGAATAC CTATTGCTGA GTTTGCAGAA GGTGTTTCCC ATCCAACTAG AAAGCCTCCG
    GATTACAACG TAGCACTTCA GAGGTCTAGA ATGGTGGCAC GGACGTGTGA GACCCAGGGG
    ACGTCGGCTC AACAGCAACA GTCACATGGC CATTCAACTA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCAAACGA GTCAGATCCA CGTCTTGCTC ACTATCAGTC TCAAGGGTTT
    CCCACAGAGG AAGATGAAGA TGAACAAGTC TCTGCTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140814.1
    CDS16..4497
    Translation

    Target ORF information:

    RefSeq Version XM_015285328.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
    GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA
    TCCAGTCACT CAGGATGTAG CATCACAAGT GATTCGGGAA GCAGCAGCCT TTCTGATATC
    TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA
    GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG
    AGTAGAGATA TTGTCAGAGA TTGCTTGGAG AAAGATCCAA TAGACAGGAC GGATGATGAC
    ATTGAACAAC TACTGGAATT CATGCATCAG TTGCCTGCTT TTGCCAACAT GACAATGTCA
    GTGAGGAGAG AGCTTTGTGC TGTAATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT
    GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTCTGAATGG CTCTGTGGAA
    GTGACATATC CTGATGGAAG AACAGAGATA CTGTGCATGG GAAACAGTTT TGGAGTTTCC
    CCTACCATGG AAAAGGAATA TATGAAGGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTTTGTA TAGCTCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAAAACATG
    CAGAAGGTAG AAGAGGAAGG AGAGATTGTG ATGGTTAAGG AGCACAGAGA GCTTGATCGC
    ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CGGAAAGGTT AACAATGCAT
    TTGGTGGAAG AACACTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACGTAT
    CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGGAAAAAGT TGTTGGAGTG GTTCAATGAC
    CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT
    GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG
    AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGCGCCGC TAAAGCAAAA
    AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG
    GGAGGGTCAG AAAAGGGATT TGGGATCTTT GTTGATAGCG TAGATTTTGG TAGTAAAGCT
    ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATATTGGAAG TGAATGGTCA AAACTTCGAA
    AATGTTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT
    GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTTTTAACAC GGTTGTCAGA GGAAAAACGA
    AATGGTGCTC CTCACTTGCC TAAAATTGGT GATATTAAAA AAGCGAGCCG TTATTCCATC
    CCAGATCTGG CTGTTGATGT GGAACAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT
    AAAGCCAACA CAGTTGGGGG AAGAAATAAG TTAAAGAAGA TACTTGACAA GACTCGAATC
    AGCATTCTGC CTCAGAAACC ATACAAGGAT ATCTTTGGTG ACATTGGAAT TGGCCAGTCT
    CAGGATGACA GCATTGTAGG ACTGAGGCAA ACAAAGCACA TTCCTCCTGC TTTACCTGTC
    AGTGGAACCC TGTCATCCAG TAATCCTGAT CTACTGCAGT CTCATCACCG TATCTTGGAC
    TTCAATACAA CTCCAGACTT ACCAGATCAA GTTCTGAGGG TTTTCAAGGC AGATCAGCAA
    AGTCGCTACA TCATGATCAG TAAGGACACA ACAGCAAAAG AGGTGGTCAT CCAGGCCATC
    AGGGAATTTG CTCTCACTGC AACTCCTGAT GCGTATTCAC TGTGTGAAGT CTCTGTCACA
    CCTGAGGGTG TAATTAAACA AAGGAGGCTT CCTGATCAGT TATCCAAGCT TGCTGACAGG
    ATACAGCTAA GTGGCAGGTA TTACCTGAAG AACAACATGG AAACAGAAAC TCTTTGTTCA
    GATGAAGATG CTCAGGAGTT ACTAAGAGAG AGTCAAATTT CCCTACTACA GCTCAGTACC
    GTTGAAGTGG CTACCCAGCT CTCCATGAGA AATTTTGAGC TATTCCGTAA TATTGAACCC
    ACAGAATACA TAGATGACTT GTTTAAACTC AAATCAAAAA CAGGCTGCAC TAATCTAAAG
    AGGTTCGAAG AAGTGATAAA TCAAGAAACA TTCTGGGTAG CTTCGGAGAT TCTAAGAGAA
    ACCAACCAGT TGAAAAGGAT GAAAATCATC AAGCATTTCA TTAAGATAGC GCTACACTGC
    AGAGAATGCA AGAATTTCAA CTCAATGTTT GCTATCATTA GTGGCTTGAA TTTGGCACCG
    GTTGCAAGGC TCCGAACTAC TTGGGAAAAA CTTCCAAGCA AGTATGAAAA GCTGTTCCAA
    GATCTACAGG ATTTGTTTGA TCCATCTAGG AATATGGCAA AATACCGTAA TGTCCTTAAC
    AGCCAAAATC TGCAGCCCCC TATTATTCCT CTGTTTCCTG TTATCAAAAA AGACCTTACA
    TTCCTTCATG AAGGAAACGA TTCCAAAGTG GAGGGCTTAG TCAACTTTGA GAAGCTGAGA
    ATGATTGCAA AGGAAATTCG CCACGTTGGT CGCATGGCAT CTGTCAACAT GGATCCTGCT
    TTAATGTTTA GAACTAGGAA GAAGAAATGG AGGAGTTTGG GGTCTCTCAG CCAGGGCAGT
    ACAAATGCAG CTGTGCTTGA TGTTGCACAA GCAGGGGGTC ATAAAAAGCG GGTCCGTCGC
    AGCTCCTTCC TTAACGCTAA AAAACTTTAT GAAGATGCTC AGATGGCACG GAAAGTAAAG
    CAGTATCTCT CAAACTTGGA TCTGGAAATG GATGAAGAAA GCCTCCAGAC TCTGTCTCTA
    CAGTGTGAGC CAGCCACCAA CACATTGCCA AAGAATACAG GAGACAAGCG ATCTGGAAAA
    TCTGAAACAT CACCGGTGGC TCCCCGGGCA GGAATTCAGC AGAAAGTGCA GCAGCAGCAG
    CAATATAAAG TAAACCAAGC ACTGCAGGTC CCTGCTGTAT CTCTTTATCC CTCTCGCAAG
    AAAGTGCCCG TCAAAGACCT CCCACCATTT GGCATTAACT CCCCACAAGC TTTAAAGAAA
    ATTCTTTCAT TATCTGAAGA AGGAAGTCTG GACCGCCACA AGAAACAATC TGAAGACACA
    GTATCAAGCG CATCTTCACA TCTGTCTTCT CCTCCCACCT CACCACAGAG TTCTCCAAGG
    AAAGGTTACG CTTTGGCTCC CAGTGGCACA GTGGATAACT TTTCAGACTC TGGTCACAGT
    GAAATTTCTT CCAGATCTAG TATTGTCAGC AATTCCTCTT TTGACTCCAT GCCAGCGTCC
    CTGCATGATG AGAGGAGACA GAGGCATTCT GTCAGCATTG TGGAGACTAA TCTTGGCGTG
    GGAAGAATTG ATAGAAGGAT CATGATTGAA CCAGATCAAT ACAGCTTAGG ATCTTACGCA
    CCACTGTCAG AGAACAGAGG CCTGTACGCT GGAGCTACTG TTCTGTCTTC TCCTAGTACA
    GAAGAACTGT CACAGGATCA GGGGGACAGA GCTTCACTCG ATGCAGCTGA CAGTGGCCGT
    GGTAGCTGGA CTTCTTGTTC AAGTGGTTCT CATGACAACA TTCAAACAAT ACAGCACCAG
    AGAAGCTGGG AGACGCTGCC TTTTGGACAC GCTCATTTTG ATAATTCAGG CGATGGGGCA
    GGACTGTGGG CCTCAGGCAG TCATGTGGAC CAGCTGATGT TCTCCGATCA TGGCACAAAG
    TACAGCCGGC AAAGTCAAGG CAGGGAGGGC CTCGATCAAG CACAGTCCAG AGCAAGCTGG
    GCATCCTCAA CGGGCTACTG GGGAGAGGAC TCGGAAGGTG ACACAGGTAC CATAAAGCGA
    AGGGGTGGGA AGGATGTTTC TGTTGAAGCT GAAACCAGCT GCATAACATC CATACCAGCA
    GAGGAGGCGA AGCCAGCTCC CATGCCCGCT CACATACCGG TAACATCAAG TACTACAAAG
    GGGCATATTG CACGAAAAGA AGGTCGGTAT CGGGAACCAC CTCCAACTCC TCCTGGTTAT
    GTAGGAATAC CTATTGCTGA GTTTGCAGAA GGTGTTTCCC ATCCAACTAG AAAGCCTCCG
    GATTACAACG TAGCACTTCA GAGGTCTAGA ATGGTGGCAC GGACGTGTGA GACCCAGGGG
    ACGTCGGCTC AACAGCAACA GTCACATGGC CATTCAACTA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCAAACGA GTCAGATCCA CGTCTTGCTC ACTATCAGTC TCAAGGGTTT
    CCCACAGAGG AAGATGAAGA TGAACAAGTC TCTGCTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34942
    Clone ID Related Accession (Same CDS sequence) XM_015285321.1 , XM_015285321.2
    Accession Version XM_015285321.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG GCATTAACTC CCCACAAGCT
    TTAAAGAAAA TTCTTTCATT ATCTGAAGAA GGAAGTCTGG ACCGCCACAA GAAACAATCT
    GAAGACACAG TATCAAGCGC ATCTTCACAT CTGTCTTCTC CTCCCACCTC ACCACAGAGT
    TCTCCAAGGA AAGGTTACGC TTTGGCTCCC AGTGGCACAG TGGATAACTT TTCAGACTCT
    GGTCACAGTG AAATTTCTTC CAGATCTAGT ATTGTCAGCA ATTCCTCTTT TGACTCCATG
    CCAGCGTCCC TGCATGATGA GAGGAGACAG AGGCATTCTG TCAGCATTGT GGAGACTAAT
    CTTGGCGTGG GAAGAATTGA TAGAAGGATC ATGATTGAAC CAGATCAATA CAGCTTAGGA
    TCTTACGCAC CACTGTCAGA GAACAGAGGC CTGTACGCTG GAGCTACTGT TCTGTCTTCT
    CCTAGTACAG AAGAACTGTC ACAGGATCAG GGGGACAGAG CTTCACTCGA TGCAGCTGAC
    AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA AGTGGTTCTC ATGACAACAT TCAAACAATA
    CAGCACCAGA GAAGCTGGGA GACGCTGCCT TTTGGACACG CTCATTTTGA TAATTCAGGC
    GATGGGGCAG GACTGTGGGC CTCAGGCAGT CATGTGGACC AGCTGATGTT CTCCGATCAT
    GGCACAAAGT ACAGCCGGCA AAGTCAAGGC AGGGAGGGCC TCGATCAAGC ACAGTCCAGA
    GCAAGCTGGG CATCCTCAAC GGGCTACTGG GGAGAGGACT CGGAAGGTGA CACAGGTACC
    ATAAAGCGAA GGGGTGGGAA GGATGTTTCT GTTGAAGCTG AAACCAGCTG CATAACATCC
    ATACCAGCAG AGGAGGCGAA GCCAGCTCCC ATGCCCGCTC ACATACCGGT AACATCAAGT
    ACTACAAAGG GGCATATTGC ACGAAAAGAA GGTCGGTATC GGGAACCACC TCCAACTCCT
    CCTGGTTATG TAGGAATACC TATTGCTGAG TTTGCAGAAG GTGTTTCCCA TCCAACTAGA
    AAGCCTCCGG ATTACAACGT AGCACTTCAG AGGTCTAGAA TGGTGGCACG GACGTGTGAG
    ACCCAGGGGA CGTCGGCTCA ACAGCAACAG TCACATGGCC ATTCAACTAG CAGGCCTGTG
    AACAAACCTC AGTGGCATAA ACCAAACGAG TCAGATCCAC GTCTTGCTCA CTATCAGTCT
    CAAGGGTTTC CCACAGAGGA AGATGAAGAT GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140807.1
    CDS368..5338
    Translation

    Target ORF information:

    RefSeq Version XM_015285321.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG GCATTAACTC CCCACAAGCT
    TTAAAGAAAA TTCTTTCATT ATCTGAAGAA GGAAGTCTGG ACCGCCACAA GAAACAATCT
    GAAGACACAG TATCAAGCGC ATCTTCACAT CTGTCTTCTC CTCCCACCTC ACCACAGAGT
    TCTCCAAGGA AAGGTTACGC TTTGGCTCCC AGTGGCACAG TGGATAACTT TTCAGACTCT
    GGTCACAGTG AAATTTCTTC CAGATCTAGT ATTGTCAGCA ATTCCTCTTT TGACTCCATG
    CCAGCGTCCC TGCATGATGA GAGGAGACAG AGGCATTCTG TCAGCATTGT GGAGACTAAT
    CTTGGCGTGG GAAGAATTGA TAGAAGGATC ATGATTGAAC CAGATCAATA CAGCTTAGGA
    TCTTACGCAC CACTGTCAGA GAACAGAGGC CTGTACGCTG GAGCTACTGT TCTGTCTTCT
    CCTAGTACAG AAGAACTGTC ACAGGATCAG GGGGACAGAG CTTCACTCGA TGCAGCTGAC
    AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA AGTGGTTCTC ATGACAACAT TCAAACAATA
    CAGCACCAGA GAAGCTGGGA GACGCTGCCT TTTGGACACG CTCATTTTGA TAATTCAGGC
    GATGGGGCAG GACTGTGGGC CTCAGGCAGT CATGTGGACC AGCTGATGTT CTCCGATCAT
    GGCACAAAGT ACAGCCGGCA AAGTCAAGGC AGGGAGGGCC TCGATCAAGC ACAGTCCAGA
    GCAAGCTGGG CATCCTCAAC GGGCTACTGG GGAGAGGACT CGGAAGGTGA CACAGGTACC
    ATAAAGCGAA GGGGTGGGAA GGATGTTTCT GTTGAAGCTG AAACCAGCTG CATAACATCC
    ATACCAGCAG AGGAGGCGAA GCCAGCTCCC ATGCCCGCTC ACATACCGGT AACATCAAGT
    ACTACAAAGG GGCATATTGC ACGAAAAGAA GGTCGGTATC GGGAACCACC TCCAACTCCT
    CCTGGTTATG TAGGAATACC TATTGCTGAG TTTGCAGAAG GTGTTTCCCA TCCAACTAGA
    AAGCCTCCGG ATTACAACGT AGCACTTCAG AGGTCTAGAA TGGTGGCACG GACGTGTGAG
    ACCCAGGGGA CGTCGGCTCA ACAGCAACAG TCACATGGCC ATTCAACTAG CAGGCCTGTG
    AACAAACCTC AGTGGCATAA ACCAAACGAG TCAGATCCAC GTCTTGCTCA CTATCAGTCT
    CAAGGGTTTC CCACAGAGGA AGATGAAGAT GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34942
    Clone ID Related Accession (Same CDS sequence) XM_015285321.1 , XM_015285321.2
    Accession Version XM_015285321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG GCATTAACTC CCCACAAGCT
    TTAAAGAAAA TTCTTTCATT ATCTGAAGAA GGAAGTCTGG ACCGCCACAA GAAACAATCT
    GAAGACACAG TATCAAGCGC ATCTTCACAT CTGTCTTCTC CTCCCACCTC ACCACAGAGT
    TCTCCAAGGA AAGGTTACGC TTTGGCTCCC AGTGGCACAG TGGATAACTT TTCAGACTCT
    GGTCACAGTG AAATTTCTTC CAGATCTAGT ATTGTCAGCA ATTCCTCTTT TGACTCCATG
    CCAGCGTCCC TGCATGATGA GAGGAGACAG AGGCATTCTG TCAGCATTGT GGAGACTAAT
    CTTGGCGTGG GAAGAATTGA TAGAAGGATC ATGATTGAAC CAGATCAATA CAGCTTAGGA
    TCTTACGCAC CACTGTCAGA GAACAGAGGC CTGTACGCTG GAGCTACTGT TCTGTCTTCT
    CCTAGTACAG AAGAACTGTC ACAGGATCAG GGGGACAGAG CTTCACTCGA TGCAGCTGAC
    AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA AGTGGTTCTC ATGACAACAT TCAAACAATA
    CAGCACCAGA GAAGCTGGGA GACGCTGCCT TTTGGACACG CTCATTTTGA TAATTCAGGC
    GATGGGGCAG GACTGTGGGC CTCAGGCAGT CATGTGGACC AGCTGATGTT CTCCGATCAT
    GGCACAAAGT ACAGCCGGCA AAGTCAAGGC AGGGAGGGCC TCGATCAAGC ACAGTCCAGA
    GCAAGCTGGG CATCCTCAAC GGGCTACTGG GGAGAGGACT CGGAAGGTGA CACAGGTACC
    ATAAAGCGAA GGGGTGGGAA GGATGTTTCT GTTGAAGCTG AAACCAGCTG CATAACATCC
    ATACCAGCAG AGGAGGCGAA GCCAGCTCCC ATGCCCGCTC ACATACCGGT AACATCAAGT
    ACTACAAAGG GGCATATTGC ACGAAAAGAA GGTCGGTATC GGGAACCACC TCCAACTCCT
    CCTGGTTATG TAGGAATACC TATTGCTGAG TTTGCAGAAG GTGTTTCCCA TCCAACTAGA
    AAGCCTCCGG ATTACAACGT AGCACTTCAG AGGTCTAGAA TGGTGGCACG GACGTGTGAG
    ACCCAGGGGA CGTCGGCTCA ACAGCAACAG TCACATGGCC ATTCAACTAG CAGGCCTGTG
    AACAAACCTC AGTGGCATAA ACCAAACGAG TCAGATCCAC GTCTTGCTCA CTATCAGTCT
    CAAGGGTTTC CCACAGAGGA AGATGAAGAT GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140807.1
    CDS368..5338
    Translation

    Target ORF information:

    RefSeq Version XM_015285321.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG GCATTAACTC CCCACAAGCT
    TTAAAGAAAA TTCTTTCATT ATCTGAAGAA GGAAGTCTGG ACCGCCACAA GAAACAATCT
    GAAGACACAG TATCAAGCGC ATCTTCACAT CTGTCTTCTC CTCCCACCTC ACCACAGAGT
    TCTCCAAGGA AAGGTTACGC TTTGGCTCCC AGTGGCACAG TGGATAACTT TTCAGACTCT
    GGTCACAGTG AAATTTCTTC CAGATCTAGT ATTGTCAGCA ATTCCTCTTT TGACTCCATG
    CCAGCGTCCC TGCATGATGA GAGGAGACAG AGGCATTCTG TCAGCATTGT GGAGACTAAT
    CTTGGCGTGG GAAGAATTGA TAGAAGGATC ATGATTGAAC CAGATCAATA CAGCTTAGGA
    TCTTACGCAC CACTGTCAGA GAACAGAGGC CTGTACGCTG GAGCTACTGT TCTGTCTTCT
    CCTAGTACAG AAGAACTGTC ACAGGATCAG GGGGACAGAG CTTCACTCGA TGCAGCTGAC
    AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA AGTGGTTCTC ATGACAACAT TCAAACAATA
    CAGCACCAGA GAAGCTGGGA GACGCTGCCT TTTGGACACG CTCATTTTGA TAATTCAGGC
    GATGGGGCAG GACTGTGGGC CTCAGGCAGT CATGTGGACC AGCTGATGTT CTCCGATCAT
    GGCACAAAGT ACAGCCGGCA AAGTCAAGGC AGGGAGGGCC TCGATCAAGC ACAGTCCAGA
    GCAAGCTGGG CATCCTCAAC GGGCTACTGG GGAGAGGACT CGGAAGGTGA CACAGGTACC
    ATAAAGCGAA GGGGTGGGAA GGATGTTTCT GTTGAAGCTG AAACCAGCTG CATAACATCC
    ATACCAGCAG AGGAGGCGAA GCCAGCTCCC ATGCCCGCTC ACATACCGGT AACATCAAGT
    ACTACAAAGG GGCATATTGC ACGAAAAGAA GGTCGGTATC GGGAACCACC TCCAACTCCT
    CCTGGTTATG TAGGAATACC TATTGCTGAG TTTGCAGAAG GTGTTTCCCA TCCAACTAGA
    AAGCCTCCGG ATTACAACGT AGCACTTCAG AGGTCTAGAA TGGTGGCACG GACGTGTGAG
    ACCCAGGGGA CGTCGGCTCA ACAGCAACAG TCACATGGCC ATTCAACTAG CAGGCCTGTG
    AACAAACCTC AGTGGCATAA ACCAAACGAG TCAGATCCAC GTCTTGCTCA CTATCAGTCT
    CAAGGGTTTC CCACAGAGGA AGATGAAGAT GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34943
    Clone ID Related Accession (Same CDS sequence) XM_004940909.3 , XM_004940909.2
    Accession Version XM_004940909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAATGACA TTGGAATTGG CCAGTCTCAG
    GATGACAGCA TTGTAGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT
    GGAACCCTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGTAT CTTGGACTTC
    AATACAACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT
    CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAGG TGGTCATCCA GGCCATCAGG
    GAATTTGCTC TCACTGCAAC TCCTGATGCG TATTCACTGT GTGAAGTCTC TGTCACACCT
    GAGGGTGTAA TTAAACAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA
    CAGCTAAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTACT AAGAGAGAGT CAAATTTCCC TACTACAGCT CAGTACCGTT
    GAAGTGGCTA CCCAGCTCTC CATGAGAAAT TTTGAGCTAT TCCGTAATAT TGAACCCACA
    GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GCTGCACTAA TCTAAAGAGG
    TTCGAAGAAG TGATAAATCA AGAAACATTC TGGGTAGCTT CGGAGATTCT AAGAGAAACC
    AACCAGTTGA AAAGGATGAA AATCATCAAG CATTTCATTA AGATAGCGCT ACACTGCAGA
    GAATGCAAGA ATTTCAACTC AATGTTTGCT ATCATTAGTG GCTTGAATTT GGCACCGGTT
    GCAAGGCTCC GAACTACTTG GGAAAAACTT CCAAGCAAGT ATGAAAAGCT GTTCCAAGAT
    CTACAGGATT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC
    CAAAATCTGC AGCCCCCTAT TATTCCTCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC
    CTTCATGAAG GAAACGATTC CAAAGTGGAG GGCTTAGTCA ACTTTGAGAA GCTGAGAATG
    ATTGCAAAGG AAATTCGCCA CGTTGGTCGC ATGGCATCTG TCAACATGGA TCCTGCTTTA
    ATGTTTAGAA CTAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA
    AATGCAGCTG TGCTTGATGT TGCACAAGCA GGGGGTCATA AAAAGCGGGT CCGTCGCAGC
    TCCTTCCTTA ACGCTAAAAA ACTTTATGAA GATGCTCAGA TGGCACGGAA AGTAAAGCAG
    TATCTCTCAA ACTTGGATCT GGAAATGGAT GAAGAAAGCC TCCAGACTCT GTCTCTACAG
    TGTGAGCCAG CCACCAACAC ATTGCCAAAG AATACAGGAG ACAAGCGATC TGGAAAATCT
    GAAACATCAC CGGTGGCTCC CCGGGCAGGA ATTCAGCAGA AAGTGCAGCA GCAGCAGCAA
    TATAAAGTAA ACCAAGCACT GCAGGTCCCT GCTGTATCTC TTTATCCCTC TCGCAAGAAA
    GTGCCCGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT
    CTTTCATTAT CTGAAGAAGG AAGTCTGGAC CGCCACAAGA AACAATCTGA AGACACAGTA
    TCAAGCGCAT CTTCACATCT GTCTTCTCCT CCCACCTCAC CACAGAGTTC TCCAAGGAAA
    GGTTACGCTT TGGCTCCCAG TGGCACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA
    ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCCATGCC AGCGTCCCTG
    CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACTAATCT TGGCGTGGGA
    AGAATTGATA GAAGGATCAT GATTGAACCA GATCAATACA GCTTAGGATC TTACGCACCA
    CTGTCAGAGA ACAGAGGCCT GTACGCTGGA GCTACTGTTC TGTCTTCTCC TAGTACAGAA
    GAACTGTCAC AGGATCAGGG GGACAGAGCT TCACTCGATG CAGCTGACAG TGGCCGTGGT
    AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAACATTC AAACAATACA GCACCAGAGA
    AGCTGGGAGA CGCTGCCTTT TGGACACGCT CATTTTGATA ATTCAGGCGA TGGGGCAGGA
    CTGTGGGCCT CAGGCAGTCA TGTGGACCAG CTGATGTTCT CCGATCATGG CACAAAGTAC
    AGCCGGCAAA GTCAAGGCAG GGAGGGCCTC GATCAAGCAC AGTCCAGAGC AAGCTGGGCA
    TCCTCAACGG GCTACTGGGG AGAGGACTCG GAAGGTGACA CAGGTACCAT AAAGCGAAGG
    GGTGGGAAGG ATGTTTCTGT TGAAGCTGAA ACCAGCTGCA TAACATCCAT ACCAGCAGAG
    GAGGCGAAGC CAGCTCCCAT GCCCGCTCAC ATACCGGTAA CATCAAGTAC TACAAAGGGG
    CATATTGCAC GAAAAGAAGG TCGGTATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA
    GGAATACCTA TTGCTGAGTT TGCAGAAGGT GTTTCCCATC CAACTAGAAA GCCTCCGGAT
    TACAACGTAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CGTGTGAGAC CCAGGGGACG
    TCGGCTCAAC AGCAACAGTC ACATGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CAAACGAGTC AGATCCACGT CTTGCTCACT ATCAGTCTCA AGGGTTTCCC
    ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004940966.2
    CDS368..5326
    Translation

    Target ORF information:

    RefSeq Version XM_004940909.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004940909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAATGACA TTGGAATTGG CCAGTCTCAG
    GATGACAGCA TTGTAGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT
    GGAACCCTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGTAT CTTGGACTTC
    AATACAACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT
    CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAGG TGGTCATCCA GGCCATCAGG
    GAATTTGCTC TCACTGCAAC TCCTGATGCG TATTCACTGT GTGAAGTCTC TGTCACACCT
    GAGGGTGTAA TTAAACAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA
    CAGCTAAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTACT AAGAGAGAGT CAAATTTCCC TACTACAGCT CAGTACCGTT
    GAAGTGGCTA CCCAGCTCTC CATGAGAAAT TTTGAGCTAT TCCGTAATAT TGAACCCACA
    GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GCTGCACTAA TCTAAAGAGG
    TTCGAAGAAG TGATAAATCA AGAAACATTC TGGGTAGCTT CGGAGATTCT AAGAGAAACC
    AACCAGTTGA AAAGGATGAA AATCATCAAG CATTTCATTA AGATAGCGCT ACACTGCAGA
    GAATGCAAGA ATTTCAACTC AATGTTTGCT ATCATTAGTG GCTTGAATTT GGCACCGGTT
    GCAAGGCTCC GAACTACTTG GGAAAAACTT CCAAGCAAGT ATGAAAAGCT GTTCCAAGAT
    CTACAGGATT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC
    CAAAATCTGC AGCCCCCTAT TATTCCTCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC
    CTTCATGAAG GAAACGATTC CAAAGTGGAG GGCTTAGTCA ACTTTGAGAA GCTGAGAATG
    ATTGCAAAGG AAATTCGCCA CGTTGGTCGC ATGGCATCTG TCAACATGGA TCCTGCTTTA
    ATGTTTAGAA CTAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA
    AATGCAGCTG TGCTTGATGT TGCACAAGCA GGGGGTCATA AAAAGCGGGT CCGTCGCAGC
    TCCTTCCTTA ACGCTAAAAA ACTTTATGAA GATGCTCAGA TGGCACGGAA AGTAAAGCAG
    TATCTCTCAA ACTTGGATCT GGAAATGGAT GAAGAAAGCC TCCAGACTCT GTCTCTACAG
    TGTGAGCCAG CCACCAACAC ATTGCCAAAG AATACAGGAG ACAAGCGATC TGGAAAATCT
    GAAACATCAC CGGTGGCTCC CCGGGCAGGA ATTCAGCAGA AAGTGCAGCA GCAGCAGCAA
    TATAAAGTAA ACCAAGCACT GCAGGTCCCT GCTGTATCTC TTTATCCCTC TCGCAAGAAA
    GTGCCCGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT
    CTTTCATTAT CTGAAGAAGG AAGTCTGGAC CGCCACAAGA AACAATCTGA AGACACAGTA
    TCAAGCGCAT CTTCACATCT GTCTTCTCCT CCCACCTCAC CACAGAGTTC TCCAAGGAAA
    GGTTACGCTT TGGCTCCCAG TGGCACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA
    ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCCATGCC AGCGTCCCTG
    CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACTAATCT TGGCGTGGGA
    AGAATTGATA GAAGGATCAT GATTGAACCA GATCAATACA GCTTAGGATC TTACGCACCA
    CTGTCAGAGA ACAGAGGCCT GTACGCTGGA GCTACTGTTC TGTCTTCTCC TAGTACAGAA
    GAACTGTCAC AGGATCAGGG GGACAGAGCT TCACTCGATG CAGCTGACAG TGGCCGTGGT
    AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAACATTC AAACAATACA GCACCAGAGA
    AGCTGGGAGA CGCTGCCTTT TGGACACGCT CATTTTGATA ATTCAGGCGA TGGGGCAGGA
    CTGTGGGCCT CAGGCAGTCA TGTGGACCAG CTGATGTTCT CCGATCATGG CACAAAGTAC
    AGCCGGCAAA GTCAAGGCAG GGAGGGCCTC GATCAAGCAC AGTCCAGAGC AAGCTGGGCA
    TCCTCAACGG GCTACTGGGG AGAGGACTCG GAAGGTGACA CAGGTACCAT AAAGCGAAGG
    GGTGGGAAGG ATGTTTCTGT TGAAGCTGAA ACCAGCTGCA TAACATCCAT ACCAGCAGAG
    GAGGCGAAGC CAGCTCCCAT GCCCGCTCAC ATACCGGTAA CATCAAGTAC TACAAAGGGG
    CATATTGCAC GAAAAGAAGG TCGGTATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA
    GGAATACCTA TTGCTGAGTT TGCAGAAGGT GTTTCCCATC CAACTAGAAA GCCTCCGGAT
    TACAACGTAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CGTGTGAGAC CCAGGGGACG
    TCGGCTCAAC AGCAACAGTC ACATGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CAAACGAGTC AGATCCACGT CTTGCTCACT ATCAGTCTCA AGGGTTTCCC
    ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34943
    Clone ID Related Accession (Same CDS sequence) XM_004940909.3 , XM_004940909.2
    Accession Version XM_004940909.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAATGACA TTGGAATTGG CCAGTCTCAG
    GATGACAGCA TTGTAGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT
    GGAACCCTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGTAT CTTGGACTTC
    AATACAACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT
    CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAGG TGGTCATCCA GGCCATCAGG
    GAATTTGCTC TCACTGCAAC TCCTGATGCG TATTCACTGT GTGAAGTCTC TGTCACACCT
    GAGGGTGTAA TTAAACAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA
    CAGCTAAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTACT AAGAGAGAGT CAAATTTCCC TACTACAGCT CAGTACCGTT
    GAAGTGGCTA CCCAGCTCTC CATGAGAAAT TTTGAGCTAT TCCGTAATAT TGAACCCACA
    GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GCTGCACTAA TCTAAAGAGG
    TTCGAAGAAG TGATAAATCA AGAAACATTC TGGGTAGCTT CGGAGATTCT AAGAGAAACC
    AACCAGTTGA AAAGGATGAA AATCATCAAG CATTTCATTA AGATAGCGCT ACACTGCAGA
    GAATGCAAGA ATTTCAACTC AATGTTTGCT ATCATTAGTG GCTTGAATTT GGCACCGGTT
    GCAAGGCTCC GAACTACTTG GGAAAAACTT CCAAGCAAGT ATGAAAAGCT GTTCCAAGAT
    CTACAGGATT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC
    CAAAATCTGC AGCCCCCTAT TATTCCTCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC
    CTTCATGAAG GAAACGATTC CAAAGTGGAG GGCTTAGTCA ACTTTGAGAA GCTGAGAATG
    ATTGCAAAGG AAATTCGCCA CGTTGGTCGC ATGGCATCTG TCAACATGGA TCCTGCTTTA
    ATGTTTAGAA CTAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA
    AATGCAGCTG TGCTTGATGT TGCACAAGCA GGGGGTCATA AAAAGCGGGT CCGTCGCAGC
    TCCTTCCTTA ACGCTAAAAA ACTTTATGAA GATGCTCAGA TGGCACGGAA AGTAAAGCAG
    TATCTCTCAA ACTTGGATCT GGAAATGGAT GAAGAAAGCC TCCAGACTCT GTCTCTACAG
    TGTGAGCCAG CCACCAACAC ATTGCCAAAG AATACAGGAG ACAAGCGATC TGGAAAATCT
    GAAACATCAC CGGTGGCTCC CCGGGCAGGA ATTCAGCAGA AAGTGCAGCA GCAGCAGCAA
    TATAAAGTAA ACCAAGCACT GCAGGTCCCT GCTGTATCTC TTTATCCCTC TCGCAAGAAA
    GTGCCCGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT
    CTTTCATTAT CTGAAGAAGG AAGTCTGGAC CGCCACAAGA AACAATCTGA AGACACAGTA
    TCAAGCGCAT CTTCACATCT GTCTTCTCCT CCCACCTCAC CACAGAGTTC TCCAAGGAAA
    GGTTACGCTT TGGCTCCCAG TGGCACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA
    ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCCATGCC AGCGTCCCTG
    CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACTAATCT TGGCGTGGGA
    AGAATTGATA GAAGGATCAT GATTGAACCA GATCAATACA GCTTAGGATC TTACGCACCA
    CTGTCAGAGA ACAGAGGCCT GTACGCTGGA GCTACTGTTC TGTCTTCTCC TAGTACAGAA
    GAACTGTCAC AGGATCAGGG GGACAGAGCT TCACTCGATG CAGCTGACAG TGGCCGTGGT
    AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAACATTC AAACAATACA GCACCAGAGA
    AGCTGGGAGA CGCTGCCTTT TGGACACGCT CATTTTGATA ATTCAGGCGA TGGGGCAGGA
    CTGTGGGCCT CAGGCAGTCA TGTGGACCAG CTGATGTTCT CCGATCATGG CACAAAGTAC
    AGCCGGCAAA GTCAAGGCAG GGAGGGCCTC GATCAAGCAC AGTCCAGAGC AAGCTGGGCA
    TCCTCAACGG GCTACTGGGG AGAGGACTCG GAAGGTGACA CAGGTACCAT AAAGCGAAGG
    GGTGGGAAGG ATGTTTCTGT TGAAGCTGAA ACCAGCTGCA TAACATCCAT ACCAGCAGAG
    GAGGCGAAGC CAGCTCCCAT GCCCGCTCAC ATACCGGTAA CATCAAGTAC TACAAAGGGG
    CATATTGCAC GAAAAGAAGG TCGGTATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA
    GGAATACCTA TTGCTGAGTT TGCAGAAGGT GTTTCCCATC CAACTAGAAA GCCTCCGGAT
    TACAACGTAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CGTGTGAGAC CCAGGGGACG
    TCGGCTCAAC AGCAACAGTC ACATGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CAAACGAGTC AGATCCACGT CTTGCTCACT ATCAGTCTCA AGGGTTTCCC
    ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004940966.2
    CDS368..5326
    Translation

    Target ORF information:

    RefSeq Version XM_004940909.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004940909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAATGACA TTGGAATTGG CCAGTCTCAG
    GATGACAGCA TTGTAGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT
    GGAACCCTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGTAT CTTGGACTTC
    AATACAACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT
    CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAGG TGGTCATCCA GGCCATCAGG
    GAATTTGCTC TCACTGCAAC TCCTGATGCG TATTCACTGT GTGAAGTCTC TGTCACACCT
    GAGGGTGTAA TTAAACAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA
    CAGCTAAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTACT AAGAGAGAGT CAAATTTCCC TACTACAGCT CAGTACCGTT
    GAAGTGGCTA CCCAGCTCTC CATGAGAAAT TTTGAGCTAT TCCGTAATAT TGAACCCACA
    GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GCTGCACTAA TCTAAAGAGG
    TTCGAAGAAG TGATAAATCA AGAAACATTC TGGGTAGCTT CGGAGATTCT AAGAGAAACC
    AACCAGTTGA AAAGGATGAA AATCATCAAG CATTTCATTA AGATAGCGCT ACACTGCAGA
    GAATGCAAGA ATTTCAACTC AATGTTTGCT ATCATTAGTG GCTTGAATTT GGCACCGGTT
    GCAAGGCTCC GAACTACTTG GGAAAAACTT CCAAGCAAGT ATGAAAAGCT GTTCCAAGAT
    CTACAGGATT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC
    CAAAATCTGC AGCCCCCTAT TATTCCTCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC
    CTTCATGAAG GAAACGATTC CAAAGTGGAG GGCTTAGTCA ACTTTGAGAA GCTGAGAATG
    ATTGCAAAGG AAATTCGCCA CGTTGGTCGC ATGGCATCTG TCAACATGGA TCCTGCTTTA
    ATGTTTAGAA CTAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA
    AATGCAGCTG TGCTTGATGT TGCACAAGCA GGGGGTCATA AAAAGCGGGT CCGTCGCAGC
    TCCTTCCTTA ACGCTAAAAA ACTTTATGAA GATGCTCAGA TGGCACGGAA AGTAAAGCAG
    TATCTCTCAA ACTTGGATCT GGAAATGGAT GAAGAAAGCC TCCAGACTCT GTCTCTACAG
    TGTGAGCCAG CCACCAACAC ATTGCCAAAG AATACAGGAG ACAAGCGATC TGGAAAATCT
    GAAACATCAC CGGTGGCTCC CCGGGCAGGA ATTCAGCAGA AAGTGCAGCA GCAGCAGCAA
    TATAAAGTAA ACCAAGCACT GCAGGTCCCT GCTGTATCTC TTTATCCCTC TCGCAAGAAA
    GTGCCCGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT
    CTTTCATTAT CTGAAGAAGG AAGTCTGGAC CGCCACAAGA AACAATCTGA AGACACAGTA
    TCAAGCGCAT CTTCACATCT GTCTTCTCCT CCCACCTCAC CACAGAGTTC TCCAAGGAAA
    GGTTACGCTT TGGCTCCCAG TGGCACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA
    ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCCATGCC AGCGTCCCTG
    CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACTAATCT TGGCGTGGGA
    AGAATTGATA GAAGGATCAT GATTGAACCA GATCAATACA GCTTAGGATC TTACGCACCA
    CTGTCAGAGA ACAGAGGCCT GTACGCTGGA GCTACTGTTC TGTCTTCTCC TAGTACAGAA
    GAACTGTCAC AGGATCAGGG GGACAGAGCT TCACTCGATG CAGCTGACAG TGGCCGTGGT
    AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAACATTC AAACAATACA GCACCAGAGA
    AGCTGGGAGA CGCTGCCTTT TGGACACGCT CATTTTGATA ATTCAGGCGA TGGGGCAGGA
    CTGTGGGCCT CAGGCAGTCA TGTGGACCAG CTGATGTTCT CCGATCATGG CACAAAGTAC
    AGCCGGCAAA GTCAAGGCAG GGAGGGCCTC GATCAAGCAC AGTCCAGAGC AAGCTGGGCA
    TCCTCAACGG GCTACTGGGG AGAGGACTCG GAAGGTGACA CAGGTACCAT AAAGCGAAGG
    GGTGGGAAGG ATGTTTCTGT TGAAGCTGAA ACCAGCTGCA TAACATCCAT ACCAGCAGAG
    GAGGCGAAGC CAGCTCCCAT GCCCGCTCAC ATACCGGTAA CATCAAGTAC TACAAAGGGG
    CATATTGCAC GAAAAGAAGG TCGGTATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA
    GGAATACCTA TTGCTGAGTT TGCAGAAGGT GTTTCCCATC CAACTAGAAA GCCTCCGGAT
    TACAACGTAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CGTGTGAGAC CCAGGGGACG
    TCGGCTCAAC AGCAACAGTC ACATGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CAAACGAGTC AGATCCACGT CTTGCTCACT ATCAGTCTCA AGGGTTTCCC
    ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34944
    Clone ID Related Accession (Same CDS sequence) XM_015285322.2 , XM_015285322.1
    Accession Version XM_015285322.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAAGGA TATCTTTGGT GACATTGGAA TTGGCCAGTC TCAGGATGAC
    AGCATTGTAG GACTGAGGCA AACAAAGCAC ATTCCTCCTG CTTTACCTGT CAGTGGAACC
    CTGTCATCCA GTAATCCTGA TCTACTGCAG TCTCATCACC GTATCTTGGA CTTCAATACA
    ACTCCAGACT TACCAGATCA AGTTCTGAGG GTTTTCAAGG CAGATCAGCA AAGTCGCTAC
    ATCATGATCA GTAAGGACAC AACAGCAAAA GAGGTGGTCA TCCAGGCCAT CAGGGAATTT
    GCTCTCACTG CAACTCCTGA TGCGTATTCA CTGTGTGAAG TCTCTGTCAC ACCTGAGGGT
    GTAATTAAAC AAAGGAGGCT TCCTGATCAG TTATCCAAGC TTGCTGACAG GATACAGCTA
    AGTGGCAGGT ATTACCTGAA GAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TACTAAGAGA GAGTCAAATT TCCCTACTAC AGCTCAGTAC CGTTGAAGTG
    GCTACCCAGC TCTCCATGAG AAATTTTGAG CTATTCCGTA ATATTGAACC CACAGAATAC
    ATAGATGACT TGTTTAAACT CAAATCAAAA ACAGGCTGCA CTAATCTAAA GAGGTTCGAA
    GAAGTGATAA ATCAAGAAAC ATTCTGGGTA GCTTCGGAGA TTCTAAGAGA AACCAACCAG
    TTGAAAAGGA TGAAAATCAT CAAGCATTTC ATTAAGATAG CGCTACACTG CAGAGAATGC
    AAGAATTTCA ACTCAATGTT TGCTATCATT AGTGGCTTGA ATTTGGCACC GGTTGCAAGG
    CTCCGAACTA CTTGGGAAAA ACTTCCAAGC AAGTATGAAA AGCTGTTCCA AGATCTACAG
    GATTTGTTTG ATCCATCTAG GAATATGGCA AAATACCGTA ATGTCCTTAA CAGCCAAAAT
    CTGCAGCCCC CTATTATTCC TCTGTTTCCT GTTATCAAAA AAGACCTTAC ATTCCTTCAT
    GAAGGAAACG ATTCCAAAGT GGAGGGCTTA GTCAACTTTG AGAAGCTGAG AATGATTGCA
    AAGGAAATTC GCCACGTTGG TCGCATGGCA TCTGTCAACA TGGATCCTGC TTTAATGTTT
    AGAACTAGGA AGAAGAAATG GAGGAGTTTG GGGTCTCTCA GCCAGGGCAG TACAAATGCA
    GCTGTGCTTG ATGTTGCACA AGCAGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC
    CTTAACGCTA AAAAACTTTA TGAAGATGCT CAGATGGCAC GGAAAGTAAA GCAGTATCTC
    TCAAACTTGG ATCTGGAAAT GGATGAAGAA AGCCTCCAGA CTCTGTCTCT ACAGTGTGAG
    CCAGCCACCA ACACATTGCC AAAGAATACA GGAGACAAGC GATCTGGAAA ATCTGAAACA
    TCACCGGTGG CTCCCCGGGC AGGAATTCAG CAGAAAGTGC AGCAGCAGCA GCAATATAAA
    GTAAACCAAG CACTGCAGGT CCCTGCTGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCC
    GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140808.1
    CDS368..5320
    Translation

    Target ORF information:

    RefSeq Version XM_015285322.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAAGGA TATCTTTGGT GACATTGGAA TTGGCCAGTC TCAGGATGAC
    AGCATTGTAG GACTGAGGCA AACAAAGCAC ATTCCTCCTG CTTTACCTGT CAGTGGAACC
    CTGTCATCCA GTAATCCTGA TCTACTGCAG TCTCATCACC GTATCTTGGA CTTCAATACA
    ACTCCAGACT TACCAGATCA AGTTCTGAGG GTTTTCAAGG CAGATCAGCA AAGTCGCTAC
    ATCATGATCA GTAAGGACAC AACAGCAAAA GAGGTGGTCA TCCAGGCCAT CAGGGAATTT
    GCTCTCACTG CAACTCCTGA TGCGTATTCA CTGTGTGAAG TCTCTGTCAC ACCTGAGGGT
    GTAATTAAAC AAAGGAGGCT TCCTGATCAG TTATCCAAGC TTGCTGACAG GATACAGCTA
    AGTGGCAGGT ATTACCTGAA GAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TACTAAGAGA GAGTCAAATT TCCCTACTAC AGCTCAGTAC CGTTGAAGTG
    GCTACCCAGC TCTCCATGAG AAATTTTGAG CTATTCCGTA ATATTGAACC CACAGAATAC
    ATAGATGACT TGTTTAAACT CAAATCAAAA ACAGGCTGCA CTAATCTAAA GAGGTTCGAA
    GAAGTGATAA ATCAAGAAAC ATTCTGGGTA GCTTCGGAGA TTCTAAGAGA AACCAACCAG
    TTGAAAAGGA TGAAAATCAT CAAGCATTTC ATTAAGATAG CGCTACACTG CAGAGAATGC
    AAGAATTTCA ACTCAATGTT TGCTATCATT AGTGGCTTGA ATTTGGCACC GGTTGCAAGG
    CTCCGAACTA CTTGGGAAAA ACTTCCAAGC AAGTATGAAA AGCTGTTCCA AGATCTACAG
    GATTTGTTTG ATCCATCTAG GAATATGGCA AAATACCGTA ATGTCCTTAA CAGCCAAAAT
    CTGCAGCCCC CTATTATTCC TCTGTTTCCT GTTATCAAAA AAGACCTTAC ATTCCTTCAT
    GAAGGAAACG ATTCCAAAGT GGAGGGCTTA GTCAACTTTG AGAAGCTGAG AATGATTGCA
    AAGGAAATTC GCCACGTTGG TCGCATGGCA TCTGTCAACA TGGATCCTGC TTTAATGTTT
    AGAACTAGGA AGAAGAAATG GAGGAGTTTG GGGTCTCTCA GCCAGGGCAG TACAAATGCA
    GCTGTGCTTG ATGTTGCACA AGCAGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC
    CTTAACGCTA AAAAACTTTA TGAAGATGCT CAGATGGCAC GGAAAGTAAA GCAGTATCTC
    TCAAACTTGG ATCTGGAAAT GGATGAAGAA AGCCTCCAGA CTCTGTCTCT ACAGTGTGAG
    CCAGCCACCA ACACATTGCC AAAGAATACA GGAGACAAGC GATCTGGAAA ATCTGAAACA
    TCACCGGTGG CTCCCCGGGC AGGAATTCAG CAGAAAGTGC AGCAGCAGCA GCAATATAAA
    GTAAACCAAG CACTGCAGGT CCCTGCTGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCC
    GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34944
    Clone ID Related Accession (Same CDS sequence) XM_015285322.2 , XM_015285322.1
    Accession Version XM_015285322.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAAGGA TATCTTTGGT GACATTGGAA TTGGCCAGTC TCAGGATGAC
    AGCATTGTAG GACTGAGGCA AACAAAGCAC ATTCCTCCTG CTTTACCTGT CAGTGGAACC
    CTGTCATCCA GTAATCCTGA TCTACTGCAG TCTCATCACC GTATCTTGGA CTTCAATACA
    ACTCCAGACT TACCAGATCA AGTTCTGAGG GTTTTCAAGG CAGATCAGCA AAGTCGCTAC
    ATCATGATCA GTAAGGACAC AACAGCAAAA GAGGTGGTCA TCCAGGCCAT CAGGGAATTT
    GCTCTCACTG CAACTCCTGA TGCGTATTCA CTGTGTGAAG TCTCTGTCAC ACCTGAGGGT
    GTAATTAAAC AAAGGAGGCT TCCTGATCAG TTATCCAAGC TTGCTGACAG GATACAGCTA
    AGTGGCAGGT ATTACCTGAA GAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TACTAAGAGA GAGTCAAATT TCCCTACTAC AGCTCAGTAC CGTTGAAGTG
    GCTACCCAGC TCTCCATGAG AAATTTTGAG CTATTCCGTA ATATTGAACC CACAGAATAC
    ATAGATGACT TGTTTAAACT CAAATCAAAA ACAGGCTGCA CTAATCTAAA GAGGTTCGAA
    GAAGTGATAA ATCAAGAAAC ATTCTGGGTA GCTTCGGAGA TTCTAAGAGA AACCAACCAG
    TTGAAAAGGA TGAAAATCAT CAAGCATTTC ATTAAGATAG CGCTACACTG CAGAGAATGC
    AAGAATTTCA ACTCAATGTT TGCTATCATT AGTGGCTTGA ATTTGGCACC GGTTGCAAGG
    CTCCGAACTA CTTGGGAAAA ACTTCCAAGC AAGTATGAAA AGCTGTTCCA AGATCTACAG
    GATTTGTTTG ATCCATCTAG GAATATGGCA AAATACCGTA ATGTCCTTAA CAGCCAAAAT
    CTGCAGCCCC CTATTATTCC TCTGTTTCCT GTTATCAAAA AAGACCTTAC ATTCCTTCAT
    GAAGGAAACG ATTCCAAAGT GGAGGGCTTA GTCAACTTTG AGAAGCTGAG AATGATTGCA
    AAGGAAATTC GCCACGTTGG TCGCATGGCA TCTGTCAACA TGGATCCTGC TTTAATGTTT
    AGAACTAGGA AGAAGAAATG GAGGAGTTTG GGGTCTCTCA GCCAGGGCAG TACAAATGCA
    GCTGTGCTTG ATGTTGCACA AGCAGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC
    CTTAACGCTA AAAAACTTTA TGAAGATGCT CAGATGGCAC GGAAAGTAAA GCAGTATCTC
    TCAAACTTGG ATCTGGAAAT GGATGAAGAA AGCCTCCAGA CTCTGTCTCT ACAGTGTGAG
    CCAGCCACCA ACACATTGCC AAAGAATACA GGAGACAAGC GATCTGGAAA ATCTGAAACA
    TCACCGGTGG CTCCCCGGGC AGGAATTCAG CAGAAAGTGC AGCAGCAGCA GCAATATAAA
    GTAAACCAAG CACTGCAGGT CCCTGCTGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCC
    GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140808.1
    CDS368..5320
    Translation

    Target ORF information:

    RefSeq Version XM_015285322.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAAGGA TATCTTTGGT GACATTGGAA TTGGCCAGTC TCAGGATGAC
    AGCATTGTAG GACTGAGGCA AACAAAGCAC ATTCCTCCTG CTTTACCTGT CAGTGGAACC
    CTGTCATCCA GTAATCCTGA TCTACTGCAG TCTCATCACC GTATCTTGGA CTTCAATACA
    ACTCCAGACT TACCAGATCA AGTTCTGAGG GTTTTCAAGG CAGATCAGCA AAGTCGCTAC
    ATCATGATCA GTAAGGACAC AACAGCAAAA GAGGTGGTCA TCCAGGCCAT CAGGGAATTT
    GCTCTCACTG CAACTCCTGA TGCGTATTCA CTGTGTGAAG TCTCTGTCAC ACCTGAGGGT
    GTAATTAAAC AAAGGAGGCT TCCTGATCAG TTATCCAAGC TTGCTGACAG GATACAGCTA
    AGTGGCAGGT ATTACCTGAA GAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TACTAAGAGA GAGTCAAATT TCCCTACTAC AGCTCAGTAC CGTTGAAGTG
    GCTACCCAGC TCTCCATGAG AAATTTTGAG CTATTCCGTA ATATTGAACC CACAGAATAC
    ATAGATGACT TGTTTAAACT CAAATCAAAA ACAGGCTGCA CTAATCTAAA GAGGTTCGAA
    GAAGTGATAA ATCAAGAAAC ATTCTGGGTA GCTTCGGAGA TTCTAAGAGA AACCAACCAG
    TTGAAAAGGA TGAAAATCAT CAAGCATTTC ATTAAGATAG CGCTACACTG CAGAGAATGC
    AAGAATTTCA ACTCAATGTT TGCTATCATT AGTGGCTTGA ATTTGGCACC GGTTGCAAGG
    CTCCGAACTA CTTGGGAAAA ACTTCCAAGC AAGTATGAAA AGCTGTTCCA AGATCTACAG
    GATTTGTTTG ATCCATCTAG GAATATGGCA AAATACCGTA ATGTCCTTAA CAGCCAAAAT
    CTGCAGCCCC CTATTATTCC TCTGTTTCCT GTTATCAAAA AAGACCTTAC ATTCCTTCAT
    GAAGGAAACG ATTCCAAAGT GGAGGGCTTA GTCAACTTTG AGAAGCTGAG AATGATTGCA
    AAGGAAATTC GCCACGTTGG TCGCATGGCA TCTGTCAACA TGGATCCTGC TTTAATGTTT
    AGAACTAGGA AGAAGAAATG GAGGAGTTTG GGGTCTCTCA GCCAGGGCAG TACAAATGCA
    GCTGTGCTTG ATGTTGCACA AGCAGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC
    CTTAACGCTA AAAAACTTTA TGAAGATGCT CAGATGGCAC GGAAAGTAAA GCAGTATCTC
    TCAAACTTGG ATCTGGAAAT GGATGAAGAA AGCCTCCAGA CTCTGTCTCT ACAGTGTGAG
    CCAGCCACCA ACACATTGCC AAAGAATACA GGAGACAAGC GATCTGGAAA ATCTGAAACA
    TCACCGGTGG CTCCCCGGGC AGGAATTCAG CAGAAAGTGC AGCAGCAGCA GCAATATAAA
    GTAAACCAAG CACTGCAGGT CCCTGCTGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCC
    GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34945
    Clone ID Related Accession (Same CDS sequence) XM_015285323.1 , XM_015285323.2
    Accession Version XM_015285323.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140809.1
    CDS369..5321
    Translation

    Target ORF information:

    RefSeq Version XM_015285323.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285323.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34945
    Clone ID Related Accession (Same CDS sequence) XM_015285323.1 , XM_015285323.2
    Accession Version XM_015285323.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140809.1
    CDS369..5321
    Translation

    Target ORF information:

    RefSeq Version XM_015285323.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG CTTTAAAGAA AATTCTTTCA
    TTATCTGAAG AAGGAAGTCT GGACCGCCAC AAGAAACAAT CTGAAGACAC AGTATCAAGC
    GCATCTTCAC ATCTGTCTTC TCCTCCCACC TCACCACAGA GTTCTCCAAG GAAAGGTTAC
    GCTTTGGCTC CCAGTGGCAC AGTGGATAAC TTTTCAGACT CTGGTCACAG TGAAATTTCT
    TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCCA TGCCAGCGTC CCTGCATGAT
    GAGAGGAGAC AGAGGCATTC TGTCAGCATT GTGGAGACTA ATCTTGGCGT GGGAAGAATT
    GATAGAAGGA TCATGATTGA ACCAGATCAA TACAGCTTAG GATCTTACGC ACCACTGTCA
    GAGAACAGAG GCCTGTACGC TGGAGCTACT GTTCTGTCTT CTCCTAGTAC AGAAGAACTG
    TCACAGGATC AGGGGGACAG AGCTTCACTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG
    ACTTCTTGTT CAAGTGGTTC TCATGACAAC ATTCAAACAA TACAGCACCA GAGAAGCTGG
    GAGACGCTGC CTTTTGGACA CGCTCATTTT GATAATTCAG GCGATGGGGC AGGACTGTGG
    GCCTCAGGCA GTCATGTGGA CCAGCTGATG TTCTCCGATC ATGGCACAAA GTACAGCCGG
    CAAAGTCAAG GCAGGGAGGG CCTCGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCA
    ACGGGCTACT GGGGAGAGGA CTCGGAAGGT GACACAGGTA CCATAAAGCG AAGGGGTGGG
    AAGGATGTTT CTGTTGAAGC TGAAACCAGC TGCATAACAT CCATACCAGC AGAGGAGGCG
    AAGCCAGCTC CCATGCCCGC TCACATACCG GTAACATCAA GTACTACAAA GGGGCATATT
    GCACGAAAAG AAGGTCGGTA TCGGGAACCA CCTCCAACTC CTCCTGGTTA TGTAGGAATA
    CCTATTGCTG AGTTTGCAGA AGGTGTTTCC CATCCAACTA GAAAGCCTCC GGATTACAAC
    GTAGCACTTC AGAGGTCTAG AATGGTGGCA CGGACGTGTG AGACCCAGGG GACGTCGGCT
    CAACAGCAAC AGTCACATGG CCATTCAACT AGCAGGCCTG TGAACAAACC TCAGTGGCAT
    AAACCAAACG AGTCAGATCC ACGTCTTGCT CACTATCAGT CTCAAGGGTT TCCCACAGAG
    GAAGATGAAG ATGAACAAGT CTCTGCTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34946
    Clone ID Related Accession (Same CDS sequence) XM_015285324.2 , XM_015285324.1
    Accession Version XM_015285324.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGTCT CTCAGCCAGG GCAGTACAAA TGCAGCTGTG
    CTTGATGTTG CACAAGCAGG GGGTCATAAA AAGCGGGTCC GTCGCAGCTC CTTCCTTAAC
    GCTAAAAAAC TTTATGAAGA TGCTCAGATG GCACGGAAAG TAAAGCAGTA TCTCTCAAAC
    TTGGATCTGG AAATGGATGA AGAAAGCCTC CAGACTCTGT CTCTACAGTG TGAGCCAGCC
    ACCAACACAT TGCCAAAGAA TACAGGAGAC AAGCGATCTG GAAAATCTGA AACATCACCG
    GTGGCTCCCC GGGCAGGAAT TCAGCAGAAA GTGCAGCAGC AGCAGCAATA TAAAGTAAAC
    CAAGCACTGC AGGTCCCTGC TGTATCTCTT TATCCCTCTC GCAAGAAAGT GCCCGTCAAA
    GACCTCCCAC CATTTGGCAT TAACTCCCCA CAAGCTTTAA AGAAAATTCT TTCATTATCT
    GAAGAAGGAA GTCTGGACCG CCACAAGAAA CAATCTGAAG ACACAGTATC AAGCGCATCT
    TCACATCTGT CTTCTCCTCC CACCTCACCA CAGAGTTCTC CAAGGAAAGG TTACGCTTTG
    GCTCCCAGTG GCACAGTGGA TAACTTTTCA GACTCTGGTC ACAGTGAAAT TTCTTCCAGA
    TCTAGTATTG TCAGCAATTC CTCTTTTGAC TCCATGCCAG CGTCCCTGCA TGATGAGAGG
    AGACAGAGGC ATTCTGTCAG CATTGTGGAG ACTAATCTTG GCGTGGGAAG AATTGATAGA
    AGGATCATGA TTGAACCAGA TCAATACAGC TTAGGATCTT ACGCACCACT GTCAGAGAAC
    AGAGGCCTGT ACGCTGGAGC TACTGTTCTG TCTTCTCCTA GTACAGAAGA ACTGTCACAG
    GATCAGGGGG ACAGAGCTTC ACTCGATGCA GCTGACAGTG GCCGTGGTAG CTGGACTTCT
    TGTTCAAGTG GTTCTCATGA CAACATTCAA ACAATACAGC ACCAGAGAAG CTGGGAGACG
    CTGCCTTTTG GACACGCTCA TTTTGATAAT TCAGGCGATG GGGCAGGACT GTGGGCCTCA
    GGCAGTCATG TGGACCAGCT GATGTTCTCC GATCATGGCA CAAAGTACAG CCGGCAAAGT
    CAAGGCAGGG AGGGCCTCGA TCAAGCACAG TCCAGAGCAA GCTGGGCATC CTCAACGGGC
    TACTGGGGAG AGGACTCGGA AGGTGACACA GGTACCATAA AGCGAAGGGG TGGGAAGGAT
    GTTTCTGTTG AAGCTGAAAC CAGCTGCATA ACATCCATAC CAGCAGAGGA GGCGAAGCCA
    GCTCCCATGC CCGCTCACAT ACCGGTAACA TCAAGTACTA CAAAGGGGCA TATTGCACGA
    AAAGAAGGTC GGTATCGGGA ACCACCTCCA ACTCCTCCTG GTTATGTAGG AATACCTATT
    GCTGAGTTTG CAGAAGGTGT TTCCCATCCA ACTAGAAAGC CTCCGGATTA CAACGTAGCA
    CTTCAGAGGT CTAGAATGGT GGCACGGACG TGTGAGACCC AGGGGACGTC GGCTCAACAG
    CAACAGTCAC ATGGCCATTC AACTAGCAGG CCTGTGAACA AACCTCAGTG GCATAAACCA
    AACGAGTCAG ATCCACGTCT TGCTCACTAT CAGTCTCAAG GGTTTCCCAC AGAGGAAGAT
    GAAGATGAAC AAGTCTCTGC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140810.1
    CDS368..5314
    Translation

    Target ORF information:

    RefSeq Version XM_015285324.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285324.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGTCT CTCAGCCAGG GCAGTACAAA TGCAGCTGTG
    CTTGATGTTG CACAAGCAGG GGGTCATAAA AAGCGGGTCC GTCGCAGCTC CTTCCTTAAC
    GCTAAAAAAC TTTATGAAGA TGCTCAGATG GCACGGAAAG TAAAGCAGTA TCTCTCAAAC
    TTGGATCTGG AAATGGATGA AGAAAGCCTC CAGACTCTGT CTCTACAGTG TGAGCCAGCC
    ACCAACACAT TGCCAAAGAA TACAGGAGAC AAGCGATCTG GAAAATCTGA AACATCACCG
    GTGGCTCCCC GGGCAGGAAT TCAGCAGAAA GTGCAGCAGC AGCAGCAATA TAAAGTAAAC
    CAAGCACTGC AGGTCCCTGC TGTATCTCTT TATCCCTCTC GCAAGAAAGT GCCCGTCAAA
    GACCTCCCAC CATTTGGCAT TAACTCCCCA CAAGCTTTAA AGAAAATTCT TTCATTATCT
    GAAGAAGGAA GTCTGGACCG CCACAAGAAA CAATCTGAAG ACACAGTATC AAGCGCATCT
    TCACATCTGT CTTCTCCTCC CACCTCACCA CAGAGTTCTC CAAGGAAAGG TTACGCTTTG
    GCTCCCAGTG GCACAGTGGA TAACTTTTCA GACTCTGGTC ACAGTGAAAT TTCTTCCAGA
    TCTAGTATTG TCAGCAATTC CTCTTTTGAC TCCATGCCAG CGTCCCTGCA TGATGAGAGG
    AGACAGAGGC ATTCTGTCAG CATTGTGGAG ACTAATCTTG GCGTGGGAAG AATTGATAGA
    AGGATCATGA TTGAACCAGA TCAATACAGC TTAGGATCTT ACGCACCACT GTCAGAGAAC
    AGAGGCCTGT ACGCTGGAGC TACTGTTCTG TCTTCTCCTA GTACAGAAGA ACTGTCACAG
    GATCAGGGGG ACAGAGCTTC ACTCGATGCA GCTGACAGTG GCCGTGGTAG CTGGACTTCT
    TGTTCAAGTG GTTCTCATGA CAACATTCAA ACAATACAGC ACCAGAGAAG CTGGGAGACG
    CTGCCTTTTG GACACGCTCA TTTTGATAAT TCAGGCGATG GGGCAGGACT GTGGGCCTCA
    GGCAGTCATG TGGACCAGCT GATGTTCTCC GATCATGGCA CAAAGTACAG CCGGCAAAGT
    CAAGGCAGGG AGGGCCTCGA TCAAGCACAG TCCAGAGCAA GCTGGGCATC CTCAACGGGC
    TACTGGGGAG AGGACTCGGA AGGTGACACA GGTACCATAA AGCGAAGGGG TGGGAAGGAT
    GTTTCTGTTG AAGCTGAAAC CAGCTGCATA ACATCCATAC CAGCAGAGGA GGCGAAGCCA
    GCTCCCATGC CCGCTCACAT ACCGGTAACA TCAAGTACTA CAAAGGGGCA TATTGCACGA
    AAAGAAGGTC GGTATCGGGA ACCACCTCCA ACTCCTCCTG GTTATGTAGG AATACCTATT
    GCTGAGTTTG CAGAAGGTGT TTCCCATCCA ACTAGAAAGC CTCCGGATTA CAACGTAGCA
    CTTCAGAGGT CTAGAATGGT GGCACGGACG TGTGAGACCC AGGGGACGTC GGCTCAACAG
    CAACAGTCAC ATGGCCATTC AACTAGCAGG CCTGTGAACA AACCTCAGTG GCATAAACCA
    AACGAGTCAG ATCCACGTCT TGCTCACTAT CAGTCTCAAG GGTTTCCCAC AGAGGAAGAT
    GAAGATGAAC AAGTCTCTGC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34946
    Clone ID Related Accession (Same CDS sequence) XM_015285324.2 , XM_015285324.1
    Accession Version XM_015285324.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGTCT CTCAGCCAGG GCAGTACAAA TGCAGCTGTG
    CTTGATGTTG CACAAGCAGG GGGTCATAAA AAGCGGGTCC GTCGCAGCTC CTTCCTTAAC
    GCTAAAAAAC TTTATGAAGA TGCTCAGATG GCACGGAAAG TAAAGCAGTA TCTCTCAAAC
    TTGGATCTGG AAATGGATGA AGAAAGCCTC CAGACTCTGT CTCTACAGTG TGAGCCAGCC
    ACCAACACAT TGCCAAAGAA TACAGGAGAC AAGCGATCTG GAAAATCTGA AACATCACCG
    GTGGCTCCCC GGGCAGGAAT TCAGCAGAAA GTGCAGCAGC AGCAGCAATA TAAAGTAAAC
    CAAGCACTGC AGGTCCCTGC TGTATCTCTT TATCCCTCTC GCAAGAAAGT GCCCGTCAAA
    GACCTCCCAC CATTTGGCAT TAACTCCCCA CAAGCTTTAA AGAAAATTCT TTCATTATCT
    GAAGAAGGAA GTCTGGACCG CCACAAGAAA CAATCTGAAG ACACAGTATC AAGCGCATCT
    TCACATCTGT CTTCTCCTCC CACCTCACCA CAGAGTTCTC CAAGGAAAGG TTACGCTTTG
    GCTCCCAGTG GCACAGTGGA TAACTTTTCA GACTCTGGTC ACAGTGAAAT TTCTTCCAGA
    TCTAGTATTG TCAGCAATTC CTCTTTTGAC TCCATGCCAG CGTCCCTGCA TGATGAGAGG
    AGACAGAGGC ATTCTGTCAG CATTGTGGAG ACTAATCTTG GCGTGGGAAG AATTGATAGA
    AGGATCATGA TTGAACCAGA TCAATACAGC TTAGGATCTT ACGCACCACT GTCAGAGAAC
    AGAGGCCTGT ACGCTGGAGC TACTGTTCTG TCTTCTCCTA GTACAGAAGA ACTGTCACAG
    GATCAGGGGG ACAGAGCTTC ACTCGATGCA GCTGACAGTG GCCGTGGTAG CTGGACTTCT
    TGTTCAAGTG GTTCTCATGA CAACATTCAA ACAATACAGC ACCAGAGAAG CTGGGAGACG
    CTGCCTTTTG GACACGCTCA TTTTGATAAT TCAGGCGATG GGGCAGGACT GTGGGCCTCA
    GGCAGTCATG TGGACCAGCT GATGTTCTCC GATCATGGCA CAAAGTACAG CCGGCAAAGT
    CAAGGCAGGG AGGGCCTCGA TCAAGCACAG TCCAGAGCAA GCTGGGCATC CTCAACGGGC
    TACTGGGGAG AGGACTCGGA AGGTGACACA GGTACCATAA AGCGAAGGGG TGGGAAGGAT
    GTTTCTGTTG AAGCTGAAAC CAGCTGCATA ACATCCATAC CAGCAGAGGA GGCGAAGCCA
    GCTCCCATGC CCGCTCACAT ACCGGTAACA TCAAGTACTA CAAAGGGGCA TATTGCACGA
    AAAGAAGGTC GGTATCGGGA ACCACCTCCA ACTCCTCCTG GTTATGTAGG AATACCTATT
    GCTGAGTTTG CAGAAGGTGT TTCCCATCCA ACTAGAAAGC CTCCGGATTA CAACGTAGCA
    CTTCAGAGGT CTAGAATGGT GGCACGGACG TGTGAGACCC AGGGGACGTC GGCTCAACAG
    CAACAGTCAC ATGGCCATTC AACTAGCAGG CCTGTGAACA AACCTCAGTG GCATAAACCA
    AACGAGTCAG ATCCACGTCT TGCTCACTAT CAGTCTCAAG GGTTTCCCAC AGAGGAAGAT
    GAAGATGAAC AAGTCTCTGC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140810.1
    CDS368..5314
    Translation

    Target ORF information:

    RefSeq Version XM_015285324.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGTCT CTCAGCCAGG GCAGTACAAA TGCAGCTGTG
    CTTGATGTTG CACAAGCAGG GGGTCATAAA AAGCGGGTCC GTCGCAGCTC CTTCCTTAAC
    GCTAAAAAAC TTTATGAAGA TGCTCAGATG GCACGGAAAG TAAAGCAGTA TCTCTCAAAC
    TTGGATCTGG AAATGGATGA AGAAAGCCTC CAGACTCTGT CTCTACAGTG TGAGCCAGCC
    ACCAACACAT TGCCAAAGAA TACAGGAGAC AAGCGATCTG GAAAATCTGA AACATCACCG
    GTGGCTCCCC GGGCAGGAAT TCAGCAGAAA GTGCAGCAGC AGCAGCAATA TAAAGTAAAC
    CAAGCACTGC AGGTCCCTGC TGTATCTCTT TATCCCTCTC GCAAGAAAGT GCCCGTCAAA
    GACCTCCCAC CATTTGGCAT TAACTCCCCA CAAGCTTTAA AGAAAATTCT TTCATTATCT
    GAAGAAGGAA GTCTGGACCG CCACAAGAAA CAATCTGAAG ACACAGTATC AAGCGCATCT
    TCACATCTGT CTTCTCCTCC CACCTCACCA CAGAGTTCTC CAAGGAAAGG TTACGCTTTG
    GCTCCCAGTG GCACAGTGGA TAACTTTTCA GACTCTGGTC ACAGTGAAAT TTCTTCCAGA
    TCTAGTATTG TCAGCAATTC CTCTTTTGAC TCCATGCCAG CGTCCCTGCA TGATGAGAGG
    AGACAGAGGC ATTCTGTCAG CATTGTGGAG ACTAATCTTG GCGTGGGAAG AATTGATAGA
    AGGATCATGA TTGAACCAGA TCAATACAGC TTAGGATCTT ACGCACCACT GTCAGAGAAC
    AGAGGCCTGT ACGCTGGAGC TACTGTTCTG TCTTCTCCTA GTACAGAAGA ACTGTCACAG
    GATCAGGGGG ACAGAGCTTC ACTCGATGCA GCTGACAGTG GCCGTGGTAG CTGGACTTCT
    TGTTCAAGTG GTTCTCATGA CAACATTCAA ACAATACAGC ACCAGAGAAG CTGGGAGACG
    CTGCCTTTTG GACACGCTCA TTTTGATAAT TCAGGCGATG GGGCAGGACT GTGGGCCTCA
    GGCAGTCATG TGGACCAGCT GATGTTCTCC GATCATGGCA CAAAGTACAG CCGGCAAAGT
    CAAGGCAGGG AGGGCCTCGA TCAAGCACAG TCCAGAGCAA GCTGGGCATC CTCAACGGGC
    TACTGGGGAG AGGACTCGGA AGGTGACACA GGTACCATAA AGCGAAGGGG TGGGAAGGAT
    GTTTCTGTTG AAGCTGAAAC CAGCTGCATA ACATCCATAC CAGCAGAGGA GGCGAAGCCA
    GCTCCCATGC CCGCTCACAT ACCGGTAACA TCAAGTACTA CAAAGGGGCA TATTGCACGA
    AAAGAAGGTC GGTATCGGGA ACCACCTCCA ACTCCTCCTG GTTATGTAGG AATACCTATT
    GCTGAGTTTG CAGAAGGTGT TTCCCATCCA ACTAGAAAGC CTCCGGATTA CAACGTAGCA
    CTTCAGAGGT CTAGAATGGT GGCACGGACG TGTGAGACCC AGGGGACGTC GGCTCAACAG
    CAACAGTCAC ATGGCCATTC AACTAGCAGG CCTGTGAACA AACCTCAGTG GCATAAACCA
    AACGAGTCAG ATCCACGTCT TGCTCACTAT CAGTCTCAAG GGTTTCCCAC AGAGGAAGAT
    GAAGATGAAC AAGTCTCTGC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34947
    Clone ID Related Accession (Same CDS sequence) XM_420387.6 , XM_420387.5
    Accession Version XM_420387.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420387.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAATGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420387.5
    CDS368..5308
    Translation

    Target ORF information:

    RefSeq Version XM_420387.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420387.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAATGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34947
    Clone ID Related Accession (Same CDS sequence) XM_420387.6 , XM_420387.5
    Accession Version XM_420387.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420387.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAATGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420387.5
    CDS368..5308
    Translation

    Target ORF information:

    RefSeq Version XM_420387.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420387.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGCTTCC TGCAGATTTC
    ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCCAGTCAC
    TCAGGATGTA GCATCACAAG TGATTCGGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT
    ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT
    GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGAGAT
    ATTGTCAGAG ATTGCTTGGA GAAAGATCCA ATAGACAGGA CGGATGATGA CATTGAACAA
    CTACTGGAAT TCATGCATCA GTTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA
    GAGCTTTGTG CTGTAATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTGAAC
    GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTCTGAATG GCTCTGTGGA AGTGACATAT
    CCTGATGGAA GAACAGAGAT ACTGTGCATG GGAAACAGTT TTGGAGTTTC CCCTACCATG
    GAAAAGGAAT ATATGAAGGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT
    ATAGCTCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAGGTA
    GAAGAGGAAG GAGAGATTGT GATGGTTAAG GAGCACAGAG AGCTTGATCG CACTGGAACA
    AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCGGAAAGGT TAACAATGCA TTTGGTGGAA
    GAACACTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACGTA TCGGACCTTT
    CTTTCCAGCC CAATGGAAGT GGGGAAAAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA
    GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA
    ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGCGCCG CTAAAGCAAA AAGAAGATTG
    ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATCTTGCT GGGAGGGTCA
    GAAAAGGGAT TTGGGATCTT TGTTGATAGC GTAGATTTTG GTAGTAAAGC TACAGAAGCA
    GGCTTGAAAC GTGGAGACCA GATATTGGAA GTGAATGGTC AAAACTTCGA AAATGTTCAG
    CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC
    AATTTATTTG TTTTTAAAGA ACTTTTAACA CGGTTGTCAG AGGAAAAACG AAATGGTGCT
    CCTCACTTGC CTAAAATTGG TGATATTAAA AAAGCGAGCC GTTATTCCAT CCCAGATCTG
    GCTGTTGATG TGGAACAGGT AATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC
    ACAGTTGGGG GAAGAAATAA GTTAAAGAAG ATACTTGACA AGACTCGAAT CAGCATTCTG
    CCTCAGAAAC CATACAATGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34948
    Clone ID Related Accession (Same CDS sequence) XM_015285325.1 , XM_015285325.2
    Accession Version XM_015285325.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG AAGGAAGTCT GGACCGCCAC
    AAGAAACAAT CTGAAGACAC AGTATCAAGC GCATCTTCAC ATCTGTCTTC TCCTCCCACC
    TCACCACAGA GTTCTCCAAG GAAAGGTTAC GCTTTGGCTC CCAGTGGCAC AGTGGATAAC
    TTTTCAGACT CTGGTCACAG TGAAATTTCT TCCAGATCTA GTATTGTCAG CAATTCCTCT
    TTTGACTCCA TGCCAGCGTC CCTGCATGAT GAGAGGAGAC AGAGGCATTC TGTCAGCATT
    GTGGAGACTA ATCTTGGCGT GGGAAGAATT GATAGAAGGA TCATGATTGA ACCAGATCAA
    TACAGCTTAG GATCTTACGC ACCACTGTCA GAGAACAGAG GCCTGTACGC TGGAGCTACT
    GTTCTGTCTT CTCCTAGTAC AGAAGAACTG TCACAGGATC AGGGGGACAG AGCTTCACTC
    GATGCAGCTG ACAGTGGCCG TGGTAGCTGG ACTTCTTGTT CAAGTGGTTC TCATGACAAC
    ATTCAAACAA TACAGCACCA GAGAAGCTGG GAGACGCTGC CTTTTGGACA CGCTCATTTT
    GATAATTCAG GCGATGGGGC AGGACTGTGG GCCTCAGGCA GTCATGTGGA CCAGCTGATG
    TTCTCCGATC ATGGCACAAA GTACAGCCGG CAAAGTCAAG GCAGGGAGGG CCTCGATCAA
    GCACAGTCCA GAGCAAGCTG GGCATCCTCA ACGGGCTACT GGGGAGAGGA CTCGGAAGGT
    GACACAGGTA CCATAAAGCG AAGGGGTGGG AAGGATGTTT CTGTTGAAGC TGAAACCAGC
    TGCATAACAT CCATACCAGC AGAGGAGGCG AAGCCAGCTC CCATGCCCGC TCACATACCG
    GTAACATCAA GTACTACAAA GGGGCATATT GCACGAAAAG AAGGTCGGTA TCGGGAACCA
    CCTCCAACTC CTCCTGGTTA TGTAGGAATA CCTATTGCTG AGTTTGCAGA AGGTGTTTCC
    CATCCAACTA GAAAGCCTCC GGATTACAAC GTAGCACTTC AGAGGTCTAG AATGGTGGCA
    CGGACGTGTG AGACCCAGGG GACGTCGGCT CAACAGCAAC AGTCACATGG CCATTCAACT
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCAAACG AGTCAGATCC ACGTCTTGCT
    CACTATCAGT CTCAAGGGTT TCCCACAGAG GAAGATGAAG ATGAACAAGT CTCTGCTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140811.1
    CDS369..5291
    Translation

    Target ORF information:

    RefSeq Version XM_015285325.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG AAGGAAGTCT GGACCGCCAC
    AAGAAACAAT CTGAAGACAC AGTATCAAGC GCATCTTCAC ATCTGTCTTC TCCTCCCACC
    TCACCACAGA GTTCTCCAAG GAAAGGTTAC GCTTTGGCTC CCAGTGGCAC AGTGGATAAC
    TTTTCAGACT CTGGTCACAG TGAAATTTCT TCCAGATCTA GTATTGTCAG CAATTCCTCT
    TTTGACTCCA TGCCAGCGTC CCTGCATGAT GAGAGGAGAC AGAGGCATTC TGTCAGCATT
    GTGGAGACTA ATCTTGGCGT GGGAAGAATT GATAGAAGGA TCATGATTGA ACCAGATCAA
    TACAGCTTAG GATCTTACGC ACCACTGTCA GAGAACAGAG GCCTGTACGC TGGAGCTACT
    GTTCTGTCTT CTCCTAGTAC AGAAGAACTG TCACAGGATC AGGGGGACAG AGCTTCACTC
    GATGCAGCTG ACAGTGGCCG TGGTAGCTGG ACTTCTTGTT CAAGTGGTTC TCATGACAAC
    ATTCAAACAA TACAGCACCA GAGAAGCTGG GAGACGCTGC CTTTTGGACA CGCTCATTTT
    GATAATTCAG GCGATGGGGC AGGACTGTGG GCCTCAGGCA GTCATGTGGA CCAGCTGATG
    TTCTCCGATC ATGGCACAAA GTACAGCCGG CAAAGTCAAG GCAGGGAGGG CCTCGATCAA
    GCACAGTCCA GAGCAAGCTG GGCATCCTCA ACGGGCTACT GGGGAGAGGA CTCGGAAGGT
    GACACAGGTA CCATAAAGCG AAGGGGTGGG AAGGATGTTT CTGTTGAAGC TGAAACCAGC
    TGCATAACAT CCATACCAGC AGAGGAGGCG AAGCCAGCTC CCATGCCCGC TCACATACCG
    GTAACATCAA GTACTACAAA GGGGCATATT GCACGAAAAG AAGGTCGGTA TCGGGAACCA
    CCTCCAACTC CTCCTGGTTA TGTAGGAATA CCTATTGCTG AGTTTGCAGA AGGTGTTTCC
    CATCCAACTA GAAAGCCTCC GGATTACAAC GTAGCACTTC AGAGGTCTAG AATGGTGGCA
    CGGACGTGTG AGACCCAGGG GACGTCGGCT CAACAGCAAC AGTCACATGG CCATTCAACT
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCAAACG AGTCAGATCC ACGTCTTGCT
    CACTATCAGT CTCAAGGGTT TCCCACAGAG GAAGATGAAG ATGAACAAGT CTCTGCTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34948
    Clone ID Related Accession (Same CDS sequence) XM_015285325.1 , XM_015285325.2
    Accession Version XM_015285325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG AAGGAAGTCT GGACCGCCAC
    AAGAAACAAT CTGAAGACAC AGTATCAAGC GCATCTTCAC ATCTGTCTTC TCCTCCCACC
    TCACCACAGA GTTCTCCAAG GAAAGGTTAC GCTTTGGCTC CCAGTGGCAC AGTGGATAAC
    TTTTCAGACT CTGGTCACAG TGAAATTTCT TCCAGATCTA GTATTGTCAG CAATTCCTCT
    TTTGACTCCA TGCCAGCGTC CCTGCATGAT GAGAGGAGAC AGAGGCATTC TGTCAGCATT
    GTGGAGACTA ATCTTGGCGT GGGAAGAATT GATAGAAGGA TCATGATTGA ACCAGATCAA
    TACAGCTTAG GATCTTACGC ACCACTGTCA GAGAACAGAG GCCTGTACGC TGGAGCTACT
    GTTCTGTCTT CTCCTAGTAC AGAAGAACTG TCACAGGATC AGGGGGACAG AGCTTCACTC
    GATGCAGCTG ACAGTGGCCG TGGTAGCTGG ACTTCTTGTT CAAGTGGTTC TCATGACAAC
    ATTCAAACAA TACAGCACCA GAGAAGCTGG GAGACGCTGC CTTTTGGACA CGCTCATTTT
    GATAATTCAG GCGATGGGGC AGGACTGTGG GCCTCAGGCA GTCATGTGGA CCAGCTGATG
    TTCTCCGATC ATGGCACAAA GTACAGCCGG CAAAGTCAAG GCAGGGAGGG CCTCGATCAA
    GCACAGTCCA GAGCAAGCTG GGCATCCTCA ACGGGCTACT GGGGAGAGGA CTCGGAAGGT
    GACACAGGTA CCATAAAGCG AAGGGGTGGG AAGGATGTTT CTGTTGAAGC TGAAACCAGC
    TGCATAACAT CCATACCAGC AGAGGAGGCG AAGCCAGCTC CCATGCCCGC TCACATACCG
    GTAACATCAA GTACTACAAA GGGGCATATT GCACGAAAAG AAGGTCGGTA TCGGGAACCA
    CCTCCAACTC CTCCTGGTTA TGTAGGAATA CCTATTGCTG AGTTTGCAGA AGGTGTTTCC
    CATCCAACTA GAAAGCCTCC GGATTACAAC GTAGCACTTC AGAGGTCTAG AATGGTGGCA
    CGGACGTGTG AGACCCAGGG GACGTCGGCT CAACAGCAAC AGTCACATGG CCATTCAACT
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCAAACG AGTCAGATCC ACGTCTTGCT
    CACTATCAGT CTCAAGGGTT TCCCACAGAG GAAGATGAAG ATGAACAAGT CTCTGCTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140811.1
    CDS369..5291
    Translation

    Target ORF information:

    RefSeq Version XM_015285325.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGAATG CACTAAACCT
    CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCAT CCAGTCACTC AGGATGTAGC ATCACAAGTG ATTCGGGAAG CAGCAGCCTT
    TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA
    GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT
    CCTTTGATGA GTAGAGATAT TGTCAGAGAT TGCTTGGAGA AAGATCCAAT AGACAGGACG
    GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAGT TGCCTGCTTT TGCCAACATG
    ACAATGTCAG TGAGGAGAGA GCTTTGTGCT GTAATGGTGT TTGCAGTTGT GGAGAGAGCA
    GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TCTGAATGGC
    TCTGTGGAAG TGACATATCC TGATGGAAGA ACAGAGATAC TGTGCATGGG AAACAGTTTT
    GGAGTTTCCC CTACCATGGA AAAGGAATAT ATGAAGGGAG TGATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTTTGTAT AGCTCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA
    AAAAACATGC AGAAGGTAGA AGAGGAAGGA GAGATTGTGA TGGTTAAGGA GCACAGAGAG
    CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC GGAAAGGTTA
    ACAATGCATT TGGTGGAAGA ACACTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG
    TTGACGTATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG
    TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT
    CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC
    AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGCGCCGCT
    AAAGCAAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT
    ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGGATCTTTG TTGATAGCGT AGATTTTGGT
    AGTAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TATTGGAAGT GAATGGTCAA
    AACTTCGAAA ATGTTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG
    TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TTTTAACACG GTTGTCAGAG
    GAAAAACGAA ATGGTGCTCC TCACTTGCCT AAAATTGGTG ATATTAAAAA AGCGAGCCGT
    TATTCCATCC CAGATCTGGC TGTTGATGTG GAACAGGTAA TAGGATTAGA AAAAGTAAAT
    AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAGT TAAAGAAGAT ACTTGACAAG
    ACTCGAATCA GCATTCTGCC TCAGAAACCA TACAAGGATA TCTTTGGTGA CATTGGAATT
    GGCCAGTCTC AGGATGACAG CATTGTAGGA CTGAGGCAAA CAAAGCACAT TCCTCCTGCT
    TTACCTGTCA GTGGAACCCT GTCATCCAGT AATCCTGATC TACTGCAGTC TCATCACCGT
    ATCTTGGACT TCAATACAAC TCCAGACTTA CCAGATCAAG TTCTGAGGGT TTTCAAGGCA
    GATCAGCAAA GTCGCTACAT CATGATCAGT AAGGACACAA CAGCAAAAGA GGTGGTCATC
    CAGGCCATCA GGGAATTTGC TCTCACTGCA ACTCCTGATG CGTATTCACT GTGTGAAGTC
    TCTGTCACAC CTGAGGGTGT AATTAAACAA AGGAGGCTTC CTGATCAGTT ATCCAAGCTT
    GCTGACAGGA TACAGCTAAG TGGCAGGTAT TACCTGAAGA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTA CTAAGAGAGA GTCAAATTTC CCTACTACAG
    CTCAGTACCG TTGAAGTGGC TACCCAGCTC TCCATGAGAA ATTTTGAGCT ATTCCGTAAT
    ATTGAACCCA CAGAATACAT AGATGACTTG TTTAAACTCA AATCAAAAAC AGGCTGCACT
    AATCTAAAGA GGTTCGAAGA AGTGATAAAT CAAGAAACAT TCTGGGTAGC TTCGGAGATT
    CTAAGAGAAA CCAACCAGTT GAAAAGGATG AAAATCATCA AGCATTTCAT TAAGATAGCG
    CTACACTGCA GAGAATGCAA GAATTTCAAC TCAATGTTTG CTATCATTAG TGGCTTGAAT
    TTGGCACCGG TTGCAAGGCT CCGAACTACT TGGGAAAAAC TTCCAAGCAA GTATGAAAAG
    CTGTTCCAAG ATCTACAGGA TTTGTTTGAT CCATCTAGGA ATATGGCAAA ATACCGTAAT
    GTCCTTAACA GCCAAAATCT GCAGCCCCCT ATTATTCCTC TGTTTCCTGT TATCAAAAAA
    GACCTTACAT TCCTTCATGA AGGAAACGAT TCCAAAGTGG AGGGCTTAGT CAACTTTGAG
    AAGCTGAGAA TGATTGCAAA GGAAATTCGC CACGTTGGTC GCATGGCATC TGTCAACATG
    GATCCTGCTT TAATGTTTAG AACTAGGAAG AAGAAATGGA GGAGTTTGGG GTCTCTCAGC
    CAGGGCAGTA CAAATGCAGC TGTGCTTGAT GTTGCACAAG CAGGGGGTCA TAAAAAGCGG
    GTCCGTCGCA GCTCCTTCCT TAACGCTAAA AAACTTTATG AAGATGCTCA GATGGCACGG
    AAAGTAAAGC AGTATCTCTC AAACTTGGAT CTGGAAATGG ATGAAGAAAG CCTCCAGACT
    CTGTCTCTAC AGTGTGAGCC AGCCACCAAC ACATTGCCAA AGAATACAGG AGACAAGCGA
    TCTGGAAAAT CTGAAACATC ACCGGTGGCT CCCCGGGCAG GAATTCAGCA GAAAGTGCAG
    CAGCAGCAGC AATATAAAGT AAACCAAGCA CTGCAGGTCC CTGCTGTATC TCTTTATCCC
    TCTCGCAAGA AAGTGCCCGT CAAAGACCTC CCACCATTTG AAGGAAGTCT GGACCGCCAC
    AAGAAACAAT CTGAAGACAC AGTATCAAGC GCATCTTCAC ATCTGTCTTC TCCTCCCACC
    TCACCACAGA GTTCTCCAAG GAAAGGTTAC GCTTTGGCTC CCAGTGGCAC AGTGGATAAC
    TTTTCAGACT CTGGTCACAG TGAAATTTCT TCCAGATCTA GTATTGTCAG CAATTCCTCT
    TTTGACTCCA TGCCAGCGTC CCTGCATGAT GAGAGGAGAC AGAGGCATTC TGTCAGCATT
    GTGGAGACTA ATCTTGGCGT GGGAAGAATT GATAGAAGGA TCATGATTGA ACCAGATCAA
    TACAGCTTAG GATCTTACGC ACCACTGTCA GAGAACAGAG GCCTGTACGC TGGAGCTACT
    GTTCTGTCTT CTCCTAGTAC AGAAGAACTG TCACAGGATC AGGGGGACAG AGCTTCACTC
    GATGCAGCTG ACAGTGGCCG TGGTAGCTGG ACTTCTTGTT CAAGTGGTTC TCATGACAAC
    ATTCAAACAA TACAGCACCA GAGAAGCTGG GAGACGCTGC CTTTTGGACA CGCTCATTTT
    GATAATTCAG GCGATGGGGC AGGACTGTGG GCCTCAGGCA GTCATGTGGA CCAGCTGATG
    TTCTCCGATC ATGGCACAAA GTACAGCCGG CAAAGTCAAG GCAGGGAGGG CCTCGATCAA
    GCACAGTCCA GAGCAAGCTG GGCATCCTCA ACGGGCTACT GGGGAGAGGA CTCGGAAGGT
    GACACAGGTA CCATAAAGCG AAGGGGTGGG AAGGATGTTT CTGTTGAAGC TGAAACCAGC
    TGCATAACAT CCATACCAGC AGAGGAGGCG AAGCCAGCTC CCATGCCCGC TCACATACCG
    GTAACATCAA GTACTACAAA GGGGCATATT GCACGAAAAG AAGGTCGGTA TCGGGAACCA
    CCTCCAACTC CTCCTGGTTA TGTAGGAATA CCTATTGCTG AGTTTGCAGA AGGTGTTTCC
    CATCCAACTA GAAAGCCTCC GGATTACAAC GTAGCACTTC AGAGGTCTAG AATGGTGGCA
    CGGACGTGTG AGACCCAGGG GACGTCGGCT CAACAGCAAC AGTCACATGG CCATTCAACT
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCAAACG AGTCAGATCC ACGTCTTGCT
    CACTATCAGT CTCAAGGGTT TCCCACAGAG GAAGATGAAG ATGAACAAGT CTCTGCTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34949
    Clone ID Related Accession (Same CDS sequence) XM_015285326.1 , XM_015285326.2
    Accession Version XM_015285326.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGCTAC AGAAAGTGAG
    GCTGGTGATA TGGATCTCAG TGGGTTGCCA GAGACAGCAG TGGATTCTGA GGATGATGAT
    GATGAAGAAG ACATTGAAAG GGCATCAGAT CCTTTGATGA GTAGAGATAT TGTCAGAGAT
    TGCTTGGAGA AAGATCCAAT AGACAGGACG GATGATGACA TTGAACAACT ACTGGAATTC
    ATGCATCAGT TGCCTGCTTT TGCCAACATG ACAATGTCAG TGAGGAGAGA GCTTTGTGCT
    GTAATGGTGT TTGCAGTTGT GGAGAGAGCA GGAACTATTG TACTGAACGA TGGGGAAGAG
    CTGGATTCCT GGTCAGTCAT TCTGAATGGC TCTGTGGAAG TGACATATCC TGATGGAAGA
    ACAGAGATAC TGTGCATGGG AAACAGTTTT GGAGTTTCCC CTACCATGGA AAAGGAATAT
    ATGAAGGGAG TGATGAGAAC CAAAGTGGAT GACTGCCAGT TTGTTTGTAT AGCTCAGCAA
    GATTATTGCC GCATCCTCAA TCAAGTGGAA AAAAACATGC AGAAGGTAGA AGAGGAAGGA
    GAGATTGTGA TGGTTAAGGA GCACAGAGAG CTTGATCGCA CTGGAACAAG AAAAGGTCAC
    ATTGTCATCA AGGGAACAGC GGAAAGGTTA ACAATGCATT TGGTGGAAGA ACACTCTGTG
    GTAGACCCAA CATTTATAGA AGACTTCCTG TTGACGTATC GGACCTTTCT TTCCAGCCCA
    ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG TTCAATGACC CCAGCCTCAG GGATAAGGTT
    ACACGGGTAG TATTGTTGTG GGTGAACAAT CACTTCAATG ATTTTGAAGG AGATCCTGCT
    ATGACTCGAT TTCTGGAAGA ATTTGAGAAC AATTTGGAGA GGGAGAAAAT GGGTGGACAT
    TTGAGGCTGT TAAATATTGC TTGCGCCGCT AAAGCAAAAA GAAGATTGAT AACCTTAACA
    AAGCCATCTC GAGAAGCCCC TTTGCCTTTT ATCTTGCTGG GAGGGTCAGA AAAGGGATTT
    GGGATCTTTG TTGATAGCGT AGATTTTGGT AGTAAAGCTA CAGAAGCAGG CTTGAAACGT
    GGAGACCAGA TATTGGAAGT GAATGGTCAA AACTTCGAAA ATGTTCAGCT GTCAAAAGCC
    ATGGAAATCC TTAGAAATAA CACTCATCTG TCTATCACTG TGAAAACCAA TTTATTTGTT
    TTTAAAGAAC TTTTAACACG GTTGTCAGAG GAAAAACGAA ATGGTGCTCC TCACTTGCCT
    AAAATTGGTG ATATTAAAAA AGCGAGCCGT TATTCCATCC CAGATCTGGC TGTTGATGTG
    GAACAGGTAA TAGGATTAGA AAAAGTAAAT AAGAAAAGTA AAGCCAACAC AGTTGGGGGA
    AGAAATAAGT TAAAGAAGAT ACTTGACAAG ACTCGAATCA GCATTCTGCC TCAGAAACCA
    TACAAGGATA TCTTTGGTGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140812.1
    CDS368..5188
    Translation

    Target ORF information:

    RefSeq Version XM_015285326.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGCTAC AGAAAGTGAG
    GCTGGTGATA TGGATCTCAG TGGGTTGCCA GAGACAGCAG TGGATTCTGA GGATGATGAT
    GATGAAGAAG ACATTGAAAG GGCATCAGAT CCTTTGATGA GTAGAGATAT TGTCAGAGAT
    TGCTTGGAGA AAGATCCAAT AGACAGGACG GATGATGACA TTGAACAACT ACTGGAATTC
    ATGCATCAGT TGCCTGCTTT TGCCAACATG ACAATGTCAG TGAGGAGAGA GCTTTGTGCT
    GTAATGGTGT TTGCAGTTGT GGAGAGAGCA GGAACTATTG TACTGAACGA TGGGGAAGAG
    CTGGATTCCT GGTCAGTCAT TCTGAATGGC TCTGTGGAAG TGACATATCC TGATGGAAGA
    ACAGAGATAC TGTGCATGGG AAACAGTTTT GGAGTTTCCC CTACCATGGA AAAGGAATAT
    ATGAAGGGAG TGATGAGAAC CAAAGTGGAT GACTGCCAGT TTGTTTGTAT AGCTCAGCAA
    GATTATTGCC GCATCCTCAA TCAAGTGGAA AAAAACATGC AGAAGGTAGA AGAGGAAGGA
    GAGATTGTGA TGGTTAAGGA GCACAGAGAG CTTGATCGCA CTGGAACAAG AAAAGGTCAC
    ATTGTCATCA AGGGAACAGC GGAAAGGTTA ACAATGCATT TGGTGGAAGA ACACTCTGTG
    GTAGACCCAA CATTTATAGA AGACTTCCTG TTGACGTATC GGACCTTTCT TTCCAGCCCA
    ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG TTCAATGACC CCAGCCTCAG GGATAAGGTT
    ACACGGGTAG TATTGTTGTG GGTGAACAAT CACTTCAATG ATTTTGAAGG AGATCCTGCT
    ATGACTCGAT TTCTGGAAGA ATTTGAGAAC AATTTGGAGA GGGAGAAAAT GGGTGGACAT
    TTGAGGCTGT TAAATATTGC TTGCGCCGCT AAAGCAAAAA GAAGATTGAT AACCTTAACA
    AAGCCATCTC GAGAAGCCCC TTTGCCTTTT ATCTTGCTGG GAGGGTCAGA AAAGGGATTT
    GGGATCTTTG TTGATAGCGT AGATTTTGGT AGTAAAGCTA CAGAAGCAGG CTTGAAACGT
    GGAGACCAGA TATTGGAAGT GAATGGTCAA AACTTCGAAA ATGTTCAGCT GTCAAAAGCC
    ATGGAAATCC TTAGAAATAA CACTCATCTG TCTATCACTG TGAAAACCAA TTTATTTGTT
    TTTAAAGAAC TTTTAACACG GTTGTCAGAG GAAAAACGAA ATGGTGCTCC TCACTTGCCT
    AAAATTGGTG ATATTAAAAA AGCGAGCCGT TATTCCATCC CAGATCTGGC TGTTGATGTG
    GAACAGGTAA TAGGATTAGA AAAAGTAAAT AAGAAAAGTA AAGCCAACAC AGTTGGGGGA
    AGAAATAAGT TAAAGAAGAT ACTTGACAAG ACTCGAATCA GCATTCTGCC TCAGAAACCA
    TACAAGGATA TCTTTGGTGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34949
    Clone ID Related Accession (Same CDS sequence) XM_015285326.1 , XM_015285326.2
    Accession Version XM_015285326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGCTAC AGAAAGTGAG
    GCTGGTGATA TGGATCTCAG TGGGTTGCCA GAGACAGCAG TGGATTCTGA GGATGATGAT
    GATGAAGAAG ACATTGAAAG GGCATCAGAT CCTTTGATGA GTAGAGATAT TGTCAGAGAT
    TGCTTGGAGA AAGATCCAAT AGACAGGACG GATGATGACA TTGAACAACT ACTGGAATTC
    ATGCATCAGT TGCCTGCTTT TGCCAACATG ACAATGTCAG TGAGGAGAGA GCTTTGTGCT
    GTAATGGTGT TTGCAGTTGT GGAGAGAGCA GGAACTATTG TACTGAACGA TGGGGAAGAG
    CTGGATTCCT GGTCAGTCAT TCTGAATGGC TCTGTGGAAG TGACATATCC TGATGGAAGA
    ACAGAGATAC TGTGCATGGG AAACAGTTTT GGAGTTTCCC CTACCATGGA AAAGGAATAT
    ATGAAGGGAG TGATGAGAAC CAAAGTGGAT GACTGCCAGT TTGTTTGTAT AGCTCAGCAA
    GATTATTGCC GCATCCTCAA TCAAGTGGAA AAAAACATGC AGAAGGTAGA AGAGGAAGGA
    GAGATTGTGA TGGTTAAGGA GCACAGAGAG CTTGATCGCA CTGGAACAAG AAAAGGTCAC
    ATTGTCATCA AGGGAACAGC GGAAAGGTTA ACAATGCATT TGGTGGAAGA ACACTCTGTG
    GTAGACCCAA CATTTATAGA AGACTTCCTG TTGACGTATC GGACCTTTCT TTCCAGCCCA
    ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG TTCAATGACC CCAGCCTCAG GGATAAGGTT
    ACACGGGTAG TATTGTTGTG GGTGAACAAT CACTTCAATG ATTTTGAAGG AGATCCTGCT
    ATGACTCGAT TTCTGGAAGA ATTTGAGAAC AATTTGGAGA GGGAGAAAAT GGGTGGACAT
    TTGAGGCTGT TAAATATTGC TTGCGCCGCT AAAGCAAAAA GAAGATTGAT AACCTTAACA
    AAGCCATCTC GAGAAGCCCC TTTGCCTTTT ATCTTGCTGG GAGGGTCAGA AAAGGGATTT
    GGGATCTTTG TTGATAGCGT AGATTTTGGT AGTAAAGCTA CAGAAGCAGG CTTGAAACGT
    GGAGACCAGA TATTGGAAGT GAATGGTCAA AACTTCGAAA ATGTTCAGCT GTCAAAAGCC
    ATGGAAATCC TTAGAAATAA CACTCATCTG TCTATCACTG TGAAAACCAA TTTATTTGTT
    TTTAAAGAAC TTTTAACACG GTTGTCAGAG GAAAAACGAA ATGGTGCTCC TCACTTGCCT
    AAAATTGGTG ATATTAAAAA AGCGAGCCGT TATTCCATCC CAGATCTGGC TGTTGATGTG
    GAACAGGTAA TAGGATTAGA AAAAGTAAAT AAGAAAAGTA AAGCCAACAC AGTTGGGGGA
    AGAAATAAGT TAAAGAAGAT ACTTGACAAG ACTCGAATCA GCATTCTGCC TCAGAAACCA
    TACAAGGATA TCTTTGGTGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140812.1
    CDS368..5188
    Translation

    Target ORF information:

    RefSeq Version XM_015285326.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGCGG 
    AGCCCCCAGG ATTTGGAGAT TGTGTATTCA TATTTACATG GAATGGAAGC CTTGTCTAAT
    CTGAGAGAAC ATCAGCTAAG GCTGATGTGT GAAACTGTTC GATATGAAAG GCATGAAGCA
    AATGAAGTTC TGTACTACCC TGATGATATT GGTACCTGTT GGTATATCCT GTTGTCTGGC
    TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCTGAAAT GATTGTGGTT
    GATTATATGG ATGAAAATGA AGAATATTTT CAGCGTCAAG CTTCTCACAG GCAATCTCGA
    AGGAGGTTTA GGAAAATCAA CCAAAAAGGG GAGCGACAAA CAATTATTGA TACTGTTGAT
    CCTTATCCTG TGGGGAAGCC ACCTCTGCCT AGAGGCTACC ATACGGCTAC AGAAAGTGAG
    GCTGGTGATA TGGATCTCAG TGGGTTGCCA GAGACAGCAG TGGATTCTGA GGATGATGAT
    GATGAAGAAG ACATTGAAAG GGCATCAGAT CCTTTGATGA GTAGAGATAT TGTCAGAGAT
    TGCTTGGAGA AAGATCCAAT AGACAGGACG GATGATGACA TTGAACAACT ACTGGAATTC
    ATGCATCAGT TGCCTGCTTT TGCCAACATG ACAATGTCAG TGAGGAGAGA GCTTTGTGCT
    GTAATGGTGT TTGCAGTTGT GGAGAGAGCA GGAACTATTG TACTGAACGA TGGGGAAGAG
    CTGGATTCCT GGTCAGTCAT TCTGAATGGC TCTGTGGAAG TGACATATCC TGATGGAAGA
    ACAGAGATAC TGTGCATGGG AAACAGTTTT GGAGTTTCCC CTACCATGGA AAAGGAATAT
    ATGAAGGGAG TGATGAGAAC CAAAGTGGAT GACTGCCAGT TTGTTTGTAT AGCTCAGCAA
    GATTATTGCC GCATCCTCAA TCAAGTGGAA AAAAACATGC AGAAGGTAGA AGAGGAAGGA
    GAGATTGTGA TGGTTAAGGA GCACAGAGAG CTTGATCGCA CTGGAACAAG AAAAGGTCAC
    ATTGTCATCA AGGGAACAGC GGAAAGGTTA ACAATGCATT TGGTGGAAGA ACACTCTGTG
    GTAGACCCAA CATTTATAGA AGACTTCCTG TTGACGTATC GGACCTTTCT TTCCAGCCCA
    ATGGAAGTGG GGAAAAAGTT GTTGGAGTGG TTCAATGACC CCAGCCTCAG GGATAAGGTT
    ACACGGGTAG TATTGTTGTG GGTGAACAAT CACTTCAATG ATTTTGAAGG AGATCCTGCT
    ATGACTCGAT TTCTGGAAGA ATTTGAGAAC AATTTGGAGA GGGAGAAAAT GGGTGGACAT
    TTGAGGCTGT TAAATATTGC TTGCGCCGCT AAAGCAAAAA GAAGATTGAT AACCTTAACA
    AAGCCATCTC GAGAAGCCCC TTTGCCTTTT ATCTTGCTGG GAGGGTCAGA AAAGGGATTT
    GGGATCTTTG TTGATAGCGT AGATTTTGGT AGTAAAGCTA CAGAAGCAGG CTTGAAACGT
    GGAGACCAGA TATTGGAAGT GAATGGTCAA AACTTCGAAA ATGTTCAGCT GTCAAAAGCC
    ATGGAAATCC TTAGAAATAA CACTCATCTG TCTATCACTG TGAAAACCAA TTTATTTGTT
    TTTAAAGAAC TTTTAACACG GTTGTCAGAG GAAAAACGAA ATGGTGCTCC TCACTTGCCT
    AAAATTGGTG ATATTAAAAA AGCGAGCCGT TATTCCATCC CAGATCTGGC TGTTGATGTG
    GAACAGGTAA TAGGATTAGA AAAAGTAAAT AAGAAAAGTA AAGCCAACAC AGTTGGGGGA
    AGAAATAAGT TAAAGAAGAT ACTTGACAAG ACTCGAATCA GCATTCTGCC TCAGAAACCA
    TACAAGGATA TCTTTGGTGA CATTGGAATT GGCCAGTCTC AGGATGACAG CATTGTAGGA
    CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCCT GTCATCCAGT
    AATCCTGATC TACTGCAGTC TCATCACCGT ATCTTGGACT TCAATACAAC TCCAGACTTA
    CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GATCAGCAAA GTCGCTACAT CATGATCAGT
    AAGGACACAA CAGCAAAAGA GGTGGTCATC CAGGCCATCA GGGAATTTGC TCTCACTGCA
    ACTCCTGATG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT AATTAAACAA
    AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGACAGGA TACAGCTAAG TGGCAGGTAT
    TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTA
    CTAAGAGAGA GTCAAATTTC CCTACTACAG CTCAGTACCG TTGAAGTGGC TACCCAGCTC
    TCCATGAGAA ATTTTGAGCT ATTCCGTAAT ATTGAACCCA CAGAATACAT AGATGACTTG
    TTTAAACTCA AATCAAAAAC AGGCTGCACT AATCTAAAGA GGTTCGAAGA AGTGATAAAT
    CAAGAAACAT TCTGGGTAGC TTCGGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG
    AAAATCATCA AGCATTTCAT TAAGATAGCG CTACACTGCA GAGAATGCAA GAATTTCAAC
    TCAATGTTTG CTATCATTAG TGGCTTGAAT TTGGCACCGG TTGCAAGGCT CCGAACTACT
    TGGGAAAAAC TTCCAAGCAA GTATGAAAAG CTGTTCCAAG ATCTACAGGA TTTGTTTGAT
    CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAATCT GCAGCCCCCT
    ATTATTCCTC TGTTTCCTGT TATCAAAAAA GACCTTACAT TCCTTCATGA AGGAAACGAT
    TCCAAAGTGG AGGGCTTAGT CAACTTTGAG AAGCTGAGAA TGATTGCAAA GGAAATTCGC
    CACGTTGGTC GCATGGCATC TGTCAACATG GATCCTGCTT TAATGTTTAG AACTAGGAAG
    AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CAAATGCAGC TGTGCTTGAT
    GTTGCACAAG CAGGGGGTCA TAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAACGCTAAA
    AAACTTTATG AAGATGCTCA GATGGCACGG AAAGTAAAGC AGTATCTCTC AAACTTGGAT
    CTGGAAATGG ATGAAGAAAG CCTCCAGACT CTGTCTCTAC AGTGTGAGCC AGCCACCAAC
    ACATTGCCAA AGAATACAGG AGACAAGCGA TCTGGAAAAT CTGAAACATC ACCGGTGGCT
    CCCCGGGCAG GAATTCAGCA GAAAGTGCAG CAGCAGCAGC AATATAAAGT AAACCAAGCA
    CTGCAGGTCC CTGCTGTATC TCTTTATCCC TCTCGCAAGA AAGTGCCCGT CAAAGACCTC
    CCACCATTTG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA
    GGAAGTCTGG ACCGCCACAA GAAACAATCT GAAGACACAG TATCAAGCGC ATCTTCACAT
    CTGTCTTCTC CTCCCACCTC ACCACAGAGT TCTCCAAGGA AAGGTTACGC TTTGGCTCCC
    AGTGGCACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCTTC CAGATCTAGT
    ATTGTCAGCA ATTCCTCTTT TGACTCCATG CCAGCGTCCC TGCATGATGA GAGGAGACAG
    AGGCATTCTG TCAGCATTGT GGAGACTAAT CTTGGCGTGG GAAGAATTGA TAGAAGGATC
    ATGATTGAAC CAGATCAATA CAGCTTAGGA TCTTACGCAC CACTGTCAGA GAACAGAGGC
    CTGTACGCTG GAGCTACTGT TCTGTCTTCT CCTAGTACAG AAGAACTGTC ACAGGATCAG
    GGGGACAGAG CTTCACTCGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA
    AGTGGTTCTC ATGACAACAT TCAAACAATA CAGCACCAGA GAAGCTGGGA GACGCTGCCT
    TTTGGACACG CTCATTTTGA TAATTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGT
    CATGTGGACC AGCTGATGTT CTCCGATCAT GGCACAAAGT ACAGCCGGCA AAGTCAAGGC
    AGGGAGGGCC TCGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC GGGCTACTGG
    GGAGAGGACT CGGAAGGTGA CACAGGTACC ATAAAGCGAA GGGGTGGGAA GGATGTTTCT
    GTTGAAGCTG AAACCAGCTG CATAACATCC ATACCAGCAG AGGAGGCGAA GCCAGCTCCC
    ATGCCCGCTC ACATACCGGT AACATCAAGT ACTACAAAGG GGCATATTGC ACGAAAAGAA
    GGTCGGTATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG
    TTTGCAGAAG GTGTTTCCCA TCCAACTAGA AAGCCTCCGG ATTACAACGT AGCACTTCAG
    AGGTCTAGAA TGGTGGCACG GACGTGTGAG ACCCAGGGGA CGTCGGCTCA ACAGCAACAG
    TCACATGGCC ATTCAACTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAACGAG
    TCAGATCCAC GTCTTGCTCA CTATCAGTCT CAAGGGTTTC CCACAGAGGA AGATGAAGAT
    GAACAAGTCT CTGCTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07743
    Clone ID Related Accession (Same CDS sequence) XM_004940910.2 , XM_004940910.3
    Accession Version XM_004940910.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455853.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AATGACATTG GAATTGGCCA GTCTCAGGAT
    GACAGCATTG TAGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA
    ACCCTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGTATCTT GGACTTCAAT
    ACAACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC
    TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAGGTGG TCATCCAGGC CATCAGGGAA
    TTTGCTCTCA CTGCAACTCC TGATGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG
    GGTGTAATTA AACAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG
    CTAAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTACTAAG AGAGAGTCAA ATTTCCCTAC TACAGCTCAG TACCGTTGAA
    GTGGCTACCC AGCTCTCCAT GAGAAATTTT GAGCTATTCC GTAATATTGA ACCCACAGAA
    TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGCT GCACTAATCT AAAGAGGTTC
    GAAGAAGTGA TAAATCAAGA AACATTCTGG GTAGCTTCGG AGATTCTAAG AGAAACCAAC
    CAGTTGAAAA GGATGAAAAT CATCAAGCAT TTCATTAAGA TAGCGCTACA CTGCAGAGAA
    TGCAAGAATT TCAACTCAAT GTTTGCTATC ATTAGTGGCT TGAATTTGGC ACCGGTTGCA
    AGGCTCCGAA CTACTTGGGA AAAACTTCCA AGCAAGTATG AAAAGCTGTT CCAAGATCTA
    CAGGATTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA
    AATCTGCAGC CCCCTATTAT TCCTCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT
    CATGAAGGAA ACGATTCCAA AGTGGAGGGC TTAGTCAACT TTGAGAAGCT GAGAATGATT
    GCAAAGGAAA TTCGCCACGT TGGTCGCATG GCATCTGTCA ACATGGATCC TGCTTTAATG
    TTTAGAACTA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT
    GCAGCTGTGC TTGATGTTGC ACAAGCAGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC
    TTCCTTAACG CTAAAAAACT TTATGAAGAT GCTCAGATGG CACGGAAAGT AAAGCAGTAT
    CTCTCAAACT TGGATCTGGA AATGGATGAA GAAAGCCTCC AGACTCTGTC TCTACAGTGT
    GAGCCAGCCA CCAACACATT GCCAAAGAAT ACAGGAGACA AGCGATCTGG AAAATCTGAA
    ACATCACCGG TGGCTCCCCG GGCAGGAATT CAGCAGAAAG TGCAGCAGCA GCAGCAATAT
    AAAGTAAACC AAGCACTGCA GGTCCCTGCT GTATCTCTTT ATCCCTCTCG CAAGAAAGTG
    CCCGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT
    TCATTATCTG AAGAAGGAAG TCTGGACCGC CACAAGAAAC AATCTGAAGA CACAGTATCA
    AGCGCATCTT CACATCTGTC TTCTCCTCCC ACCTCACCAC AGAGTTCTCC AAGGAAAGGT
    TACGCTTTGG CTCCCAGTGG CACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT
    TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CCATGCCAGC GTCCCTGCAT
    GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CTAATCTTGG CGTGGGAAGA
    ATTGATAGAA GGATCATGAT TGAACCAGAT CAATACAGCT TAGGATCTTA CGCACCACTG
    TCAGAGAACA GAGGCCTGTA CGCTGGAGCT ACTGTTCTGT CTTCTCCTAG TACAGAAGAA
    CTGTCACAGG ATCAGGGGGA CAGAGCTTCA CTCGATGCAG CTGACAGTGG CCGTGGTAGC
    TGGACTTCTT GTTCAAGTGG TTCTCATGAC AACATTCAAA CAATACAGCA CCAGAGAAGC
    TGGGAGACGC TGCCTTTTGG ACACGCTCAT TTTGATAATT CAGGCGATGG GGCAGGACTG
    TGGGCCTCAG GCAGTCATGT GGACCAGCTG ATGTTCTCCG ATCATGGCAC AAAGTACAGC
    CGGCAAAGTC AAGGCAGGGA GGGCCTCGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC
    TCAACGGGCT ACTGGGGAGA GGACTCGGAA GGTGACACAG GTACCATAAA GCGAAGGGGT
    GGGAAGGATG TTTCTGTTGA AGCTGAAACC AGCTGCATAA CATCCATACC AGCAGAGGAG
    GCGAAGCCAG CTCCCATGCC CGCTCACATA CCGGTAACAT CAAGTACTAC AAAGGGGCAT
    ATTGCACGAA AAGAAGGTCG GTATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA
    ATACCTATTG CTGAGTTTGC AGAAGGTGTT TCCCATCCAA CTAGAAAGCC TCCGGATTAC
    AACGTAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACGT GTGAGACCCA GGGGACGTCG
    GCTCAACAGC AACAGTCACA TGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCTCAGTGG
    CATAAACCAA ACGAGTCAGA TCCACGTCTT GCTCACTATC AGTCTCAAGG GTTTCCCACA
    GAGGAAGATG AAGATGAACA AGTCTCTGCT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004940967.1
    CDS518..4993
    Translation

    Target ORF information:

    RefSeq Version XM_004940910.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004940910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AATGACATTG GAATTGGCCA GTCTCAGGAT
    GACAGCATTG TAGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA
    ACCCTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGTATCTT GGACTTCAAT
    ACAACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC
    TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAGGTGG TCATCCAGGC CATCAGGGAA
    TTTGCTCTCA CTGCAACTCC TGATGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG
    GGTGTAATTA AACAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG
    CTAAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTACTAAG AGAGAGTCAA ATTTCCCTAC TACAGCTCAG TACCGTTGAA
    GTGGCTACCC AGCTCTCCAT GAGAAATTTT GAGCTATTCC GTAATATTGA ACCCACAGAA
    TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGCT GCACTAATCT AAAGAGGTTC
    GAAGAAGTGA TAAATCAAGA AACATTCTGG GTAGCTTCGG AGATTCTAAG AGAAACCAAC
    CAGTTGAAAA GGATGAAAAT CATCAAGCAT TTCATTAAGA TAGCGCTACA CTGCAGAGAA
    TGCAAGAATT TCAACTCAAT GTTTGCTATC ATTAGTGGCT TGAATTTGGC ACCGGTTGCA
    AGGCTCCGAA CTACTTGGGA AAAACTTCCA AGCAAGTATG AAAAGCTGTT CCAAGATCTA
    CAGGATTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA
    AATCTGCAGC CCCCTATTAT TCCTCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT
    CATGAAGGAA ACGATTCCAA AGTGGAGGGC TTAGTCAACT TTGAGAAGCT GAGAATGATT
    GCAAAGGAAA TTCGCCACGT TGGTCGCATG GCATCTGTCA ACATGGATCC TGCTTTAATG
    TTTAGAACTA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT
    GCAGCTGTGC TTGATGTTGC ACAAGCAGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC
    TTCCTTAACG CTAAAAAACT TTATGAAGAT GCTCAGATGG CACGGAAAGT AAAGCAGTAT
    CTCTCAAACT TGGATCTGGA AATGGATGAA GAAAGCCTCC AGACTCTGTC TCTACAGTGT
    GAGCCAGCCA CCAACACATT GCCAAAGAAT ACAGGAGACA AGCGATCTGG AAAATCTGAA
    ACATCACCGG TGGCTCCCCG GGCAGGAATT CAGCAGAAAG TGCAGCAGCA GCAGCAATAT
    AAAGTAAACC AAGCACTGCA GGTCCCTGCT GTATCTCTTT ATCCCTCTCG CAAGAAAGTG
    CCCGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT
    TCATTATCTG AAGAAGGAAG TCTGGACCGC CACAAGAAAC AATCTGAAGA CACAGTATCA
    AGCGCATCTT CACATCTGTC TTCTCCTCCC ACCTCACCAC AGAGTTCTCC AAGGAAAGGT
    TACGCTTTGG CTCCCAGTGG CACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT
    TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CCATGCCAGC GTCCCTGCAT
    GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CTAATCTTGG CGTGGGAAGA
    ATTGATAGAA GGATCATGAT TGAACCAGAT CAATACAGCT TAGGATCTTA CGCACCACTG
    TCAGAGAACA GAGGCCTGTA CGCTGGAGCT ACTGTTCTGT CTTCTCCTAG TACAGAAGAA
    CTGTCACAGG ATCAGGGGGA CAGAGCTTCA CTCGATGCAG CTGACAGTGG CCGTGGTAGC
    TGGACTTCTT GTTCAAGTGG TTCTCATGAC AACATTCAAA CAATACAGCA CCAGAGAAGC
    TGGGAGACGC TGCCTTTTGG ACACGCTCAT TTTGATAATT CAGGCGATGG GGCAGGACTG
    TGGGCCTCAG GCAGTCATGT GGACCAGCTG ATGTTCTCCG ATCATGGCAC AAAGTACAGC
    CGGCAAAGTC AAGGCAGGGA GGGCCTCGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC
    TCAACGGGCT ACTGGGGAGA GGACTCGGAA GGTGACACAG GTACCATAAA GCGAAGGGGT
    GGGAAGGATG TTTCTGTTGA AGCTGAAACC AGCTGCATAA CATCCATACC AGCAGAGGAG
    GCGAAGCCAG CTCCCATGCC CGCTCACATA CCGGTAACAT CAAGTACTAC AAAGGGGCAT
    ATTGCACGAA AAGAAGGTCG GTATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA
    ATACCTATTG CTGAGTTTGC AGAAGGTGTT TCCCATCCAA CTAGAAAGCC TCCGGATTAC
    AACGTAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACGT GTGAGACCCA GGGGACGTCG
    GCTCAACAGC AACAGTCACA TGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCTCAGTGG
    CATAAACCAA ACGAGTCAGA TCCACGTCTT GCTCACTATC AGTCTCAAGG GTTTCCCACA
    GAGGAAGATG AAGATGAACA AGTCTCTGCT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07743
    Clone ID Related Accession (Same CDS sequence) XM_004940910.2 , XM_004940910.3
    Accession Version XM_004940910.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rap guanine nucleotide exchange factor 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AATGACATTG GAATTGGCCA GTCTCAGGAT
    GACAGCATTG TAGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA
    ACCCTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGTATCTT GGACTTCAAT
    ACAACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC
    TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAGGTGG TCATCCAGGC CATCAGGGAA
    TTTGCTCTCA CTGCAACTCC TGATGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG
    GGTGTAATTA AACAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG
    CTAAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTACTAAG AGAGAGTCAA ATTTCCCTAC TACAGCTCAG TACCGTTGAA
    GTGGCTACCC AGCTCTCCAT GAGAAATTTT GAGCTATTCC GTAATATTGA ACCCACAGAA
    TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGCT GCACTAATCT AAAGAGGTTC
    GAAGAAGTGA TAAATCAAGA AACATTCTGG GTAGCTTCGG AGATTCTAAG AGAAACCAAC
    CAGTTGAAAA GGATGAAAAT CATCAAGCAT TTCATTAAGA TAGCGCTACA CTGCAGAGAA
    TGCAAGAATT TCAACTCAAT GTTTGCTATC ATTAGTGGCT TGAATTTGGC ACCGGTTGCA
    AGGCTCCGAA CTACTTGGGA AAAACTTCCA AGCAAGTATG AAAAGCTGTT CCAAGATCTA
    CAGGATTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA
    AATCTGCAGC CCCCTATTAT TCCTCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT
    CATGAAGGAA ACGATTCCAA AGTGGAGGGC TTAGTCAACT TTGAGAAGCT GAGAATGATT
    GCAAAGGAAA TTCGCCACGT TGGTCGCATG GCATCTGTCA ACATGGATCC TGCTTTAATG
    TTTAGAACTA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT
    GCAGCTGTGC TTGATGTTGC ACAAGCAGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC
    TTCCTTAACG CTAAAAAACT TTATGAAGAT GCTCAGATGG CACGGAAAGT AAAGCAGTAT
    CTCTCAAACT TGGATCTGGA AATGGATGAA GAAAGCCTCC AGACTCTGTC TCTACAGTGT
    GAGCCAGCCA CCAACACATT GCCAAAGAAT ACAGGAGACA AGCGATCTGG AAAATCTGAA
    ACATCACCGG TGGCTCCCCG GGCAGGAATT CAGCAGAAAG TGCAGCAGCA GCAGCAATAT
    AAAGTAAACC AAGCACTGCA GGTCCCTGCT GTATCTCTTT ATCCCTCTCG CAAGAAAGTG
    CCCGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT
    TCATTATCTG AAGAAGGAAG TCTGGACCGC CACAAGAAAC AATCTGAAGA CACAGTATCA
    AGCGCATCTT CACATCTGTC TTCTCCTCCC ACCTCACCAC AGAGTTCTCC AAGGAAAGGT
    TACGCTTTGG CTCCCAGTGG CACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT
    TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CCATGCCAGC GTCCCTGCAT
    GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CTAATCTTGG CGTGGGAAGA
    ATTGATAGAA GGATCATGAT TGAACCAGAT CAATACAGCT TAGGATCTTA CGCACCACTG
    TCAGAGAACA GAGGCCTGTA CGCTGGAGCT ACTGTTCTGT CTTCTCCTAG TACAGAAGAA
    CTGTCACAGG ATCAGGGGGA CAGAGCTTCA CTCGATGCAG CTGACAGTGG CCGTGGTAGC
    TGGACTTCTT GTTCAAGTGG TTCTCATGAC AACATTCAAA CAATACAGCA CCAGAGAAGC
    TGGGAGACGC TGCCTTTTGG ACACGCTCAT TTTGATAATT CAGGCGATGG GGCAGGACTG
    TGGGCCTCAG GCAGTCATGT GGACCAGCTG ATGTTCTCCG ATCATGGCAC AAAGTACAGC
    CGGCAAAGTC AAGGCAGGGA GGGCCTCGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC
    TCAACGGGCT ACTGGGGAGA GGACTCGGAA GGTGACACAG GTACCATAAA GCGAAGGGGT
    GGGAAGGATG TTTCTGTTGA AGCTGAAACC AGCTGCATAA CATCCATACC AGCAGAGGAG
    GCGAAGCCAG CTCCCATGCC CGCTCACATA CCGGTAACAT CAAGTACTAC AAAGGGGCAT
    ATTGCACGAA AAGAAGGTCG GTATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA
    ATACCTATTG CTGAGTTTGC AGAAGGTGTT TCCCATCCAA CTAGAAAGCC TCCGGATTAC
    AACGTAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACGT GTGAGACCCA GGGGACGTCG
    GCTCAACAGC AACAGTCACA TGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCTCAGTGG
    CATAAACCAA ACGAGTCAGA TCCACGTCTT GCTCACTATC AGTCTCAAGG GTTTCCCACA
    GAGGAAGATG AAGATGAACA AGTCTCTGCT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004940967.1
    CDS517..4992
    Translation

    Target ORF information:

    RefSeq Version XM_004940910.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004940910.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
    CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCATCCA GTCACTCAGG ATGTAGCATC ACAAGTGATT CGGGAAGCAG CAGCCTTTCT
    GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG
    ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT
    TTGATGAGTA GAGATATTGT CAGAGATTGC TTGGAGAAAG ATCCAATAGA CAGGACGGAT
    GATGACATTG AACAACTACT GGAATTCATG CATCAGTTGC CTGCTTTTGC CAACATGACA
    ATGTCAGTGA GGAGAGAGCT TTGTGCTGTA ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA
    ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTCT GAATGGCTCT
    GTGGAAGTGA CATATCCTGA TGGAAGAACA GAGATACTGT GCATGGGAAA CAGTTTTGGA
    GTTTCCCCTA CCATGGAAAA GGAATATATG AAGGGAGTGA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TTTGTATAGC TCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA
    AACATGCAGA AGGTAGAAGA GGAAGGAGAG ATTGTGATGG TTAAGGAGCA CAGAGAGCTT
    GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCGGA AAGGTTAACA
    ATGCATTTGG TGGAAGAACA CTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG
    ACGTATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGGA AAAAGTTGTT GGAGTGGTTC
    AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC
    TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT
    TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG CGCCGCTAAA
    GCAAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC
    TTGCTGGGAG GGTCAGAAAA GGGATTTGGG ATCTTTGTTG ATAGCGTAGA TTTTGGTAGT
    AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAT TGGAAGTGAA TGGTCAAAAC
    TTCGAAAATG TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT
    ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTTT TAACACGGTT GTCAGAGGAA
    AAACGAAATG GTGCTCCTCA CTTGCCTAAA ATTGGTGATA TTAAAAAAGC GAGCCGTTAT
    TCCATCCCAG ATCTGGCTGT TGATGTGGAA CAGGTAATAG GATTAGAAAA AGTAAATAAG
    AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAGTTAA AGAAGATACT TGACAAGACT
    CGAATCAGCA TTCTGCCTCA GAAACCATAC AATGACATTG GAATTGGCCA GTCTCAGGAT
    GACAGCATTG TAGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA
    ACCCTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGTATCTT GGACTTCAAT
    ACAACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC
    TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAGGTGG TCATCCAGGC CATCAGGGAA
    TTTGCTCTCA CTGCAACTCC TGATGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG
    GGTGTAATTA AACAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG
    CTAAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTACTAAG AGAGAGTCAA ATTTCCCTAC TACAGCTCAG TACCGTTGAA
    GTGGCTACCC AGCTCTCCAT GAGAAATTTT GAGCTATTCC GTAATATTGA ACCCACAGAA
    TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGCT GCACTAATCT AAAGAGGTTC
    GAAGAAGTGA TAAATCAAGA AACATTCTGG GTAGCTTCGG AGATTCTAAG AGAAACCAAC
    CAGTTGAAAA GGATGAAAAT CATCAAGCAT TTCATTAAGA TAGCGCTACA CTGCAGAGAA
    TGCAAGAATT TCAACTCAAT GTTTGCTATC ATTAGTGGCT TGAATTTGGC ACCGGTTGCA
    AGGCTCCGAA CTACTTGGGA AAAACTTCCA AGCAAGTATG AAAAGCTGTT CCAAGATCTA
    CAGGATTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA
    AATCTGCAGC CCCCTATTAT TCCTCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT
    CATGAAGGAA ACGATTCCAA AGTGGAGGGC TTAGTCAACT TTGAGAAGCT GAGAATGATT
    GCAAAGGAAA TTCGCCACGT TGGTCGCATG GCATCTGTCA ACATGGATCC TGCTTTAATG
    TTTAGAACTA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT
    GCAGCTGTGC TTGATGTTGC ACAAGCAGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC
    TTCCTTAACG CTAAAAAACT TTATGAAGAT GCTCAGATGG CACGGAAAGT AAAGCAGTAT
    CTCTCAAACT TGGATCTGGA AATGGATGAA GAAAGCCTCC AGACTCTGTC TCTACAGTGT
    GAGCCAGCCA CCAACACATT GCCAAAGAAT ACAGGAGACA AGCGATCTGG AAAATCTGAA
    ACATCACCGG TGGCTCCCCG GGCAGGAATT CAGCAGAAAG TGCAGCAGCA GCAGCAATAT
    AAAGTAAACC AAGCACTGCA GGTCCCTGCT GTATCTCTTT ATCCCTCTCG CAAGAAAGTG
    CCCGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT
    TCATTATCTG AAGAAGGAAG TCTGGACCGC CACAAGAAAC AATCTGAAGA CACAGTATCA
    AGCGCATCTT CACATCTGTC TTCTCCTCCC ACCTCACCAC AGAGTTCTCC AAGGAAAGGT
    TACGCTTTGG CTCCCAGTGG CACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT
    TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CCATGCCAGC GTCCCTGCAT
    GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CTAATCTTGG CGTGGGAAGA
    ATTGATAGAA GGATCATGAT TGAACCAGAT CAATACAGCT TAGGATCTTA CGCACCACTG
    TCAGAGAACA GAGGCCTGTA CGCTGGAGCT ACTGTTCTGT CTTCTCCTAG TACAGAAGAA
    CTGTCACAGG ATCAGGGGGA CAGAGCTTCA CTCGATGCAG CTGACAGTGG CCGTGGTAGC
    TGGACTTCTT GTTCAAGTGG TTCTCATGAC AACATTCAAA CAATACAGCA CCAGAGAAGC
    TGGGAGACGC TGCCTTTTGG ACACGCTCAT TTTGATAATT CAGGCGATGG GGCAGGACTG
    TGGGCCTCAG GCAGTCATGT GGACCAGCTG ATGTTCTCCG ATCATGGCAC AAAGTACAGC
    CGGCAAAGTC AAGGCAGGGA GGGCCTCGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC
    TCAACGGGCT ACTGGGGAGA GGACTCGGAA GGTGACACAG GTACCATAAA GCGAAGGGGT
    GGGAAGGATG TTTCTGTTGA AGCTGAAACC AGCTGCATAA CATCCATACC AGCAGAGGAG
    GCGAAGCCAG CTCCCATGCC CGCTCACATA CCGGTAACAT CAAGTACTAC AAAGGGGCAT
    ATTGCACGAA AAGAAGGTCG GTATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA
    ATACCTATTG CTGAGTTTGC AGAAGGTGTT TCCCATCCAA CTAGAAAGCC TCCGGATTAC
    AACGTAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACGT GTGAGACCCA GGGGACGTCG
    GCTCAACAGC AACAGTCACA TGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCTCAGTGG
    CATAAACCAA ACGAGTCAGA TCCACGTCTT GCTCACTATC AGTCTCAAGG GTTTCCCACA
    GAGGAAGATG AAGATGAACA AGTCTCTGCT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.