rapgef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rapgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa47599 XM_031895801.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa41692 XM_018091100.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa47591 XM_012956489.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47592 XM_012956494.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47593 XM_012956500.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47594 XM_012956496.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47595 XM_012956506.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47596 XM_012956508.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47597 XM_012956514.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa47598 XM_012956519.3
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa23255 NM_001127050.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31949 XM_012956489.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31950 XM_012956494.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31951 XM_012956496.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31952 XM_012956500.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31953 XM_012956506.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31954 XM_012956508.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31955 XM_012956514.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31956 XM_012956519.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa47599
    Clone ID Related Accession (Same CDS sequence) XM_031895801.1
    Accession Version XM_031895801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTCCCT 
    GCTGATTTCA CTAAACTGCA CATCACAGAC AGTCTACATC CACAGGTGAC GCACGTGTCC
    TCTAGTCATT CAGGATGTAG CATCACTAGC GATTCTGGGA GCAGCAGCTT GTCTGACATC
    TACCAGGCCA CAGAAAGTGA GCCAGGAGAT ATGGACCTGA GTGGGCTGCC AGAGACCGCT
    GTAGACTCAG AAGATGACGA TGATGAAGAG GATATTGAAA GAGCTTCTGA CCCTTTAATG
    AGCAGGGACA TTGTCAGAGA CTGTCTGGAG AAGGATCCAA TTGACAGGAC TGACGACGAT
    ATTGAACAAC TTCTGGAGTT CATGCACCAG TTGCCAGCAT TTGCCAACAT GACAATGTCT
    GTAAGACGAG AGCTCTGTGC AGTCATGGTG TTCGCTGTGG TCGAGCGGGC AGGAACTGTT
    GTACTAAATG ATGGGGAGGA GCTCGATTCC TGGTCAGTTA TTTTAAATGG CTCAGTAGAA
    GTGACATATC CTGATGGCAG GACAGAAATA CTTTGCATGG GGAACAGCTT TGGTGTGTCG
    CCAACCATGG AGAAGGAATA CATGAAGGGA GTCATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTGTGCA TCGCCCAGCA AGATTACTGC CGCATCCTCA ATCAGGTGGA AAAGAACATG
    CAGAAAGTGG AAGAGGAGGG TGAGATTGTG ATGGTGAAAG AGCACAGAGA GCTTGATCGC
    ACCGGAACCA GGAAAGGTCA TATTGTCATC AAGGGAACAT CGGAACGCCT AACCATGCAT
    CTTGTGGAAG AACATTCTGT GGTTGACCCA ACATATATTG AAGATTTTCT CCTCACTTAC
    AGAACTTTCC TTTCTAGTCC GATGGAAGTG GGAAAGAAAC TACTGGAGTG GTTTAATGAC
    CCTAGTCTCA GGGACAAGGT TACACGAGTA GTGTTGCTCT GGGTAAATAA TCACTTTAAT
    GACTTTGAAG GGGACCCAGC CATGACTCGA TTTTTGGAAG AATTTGAGAA CAATTTGGAA
    CGTGAGAAAA TGGGTGGCCA CCTAAGGCTA TTAAATATTG CATGTGCTGC CAAAGCAAAA
    AGAAGATTGA TAACTCTAAC AAAGCCCTCC CGAGAAGCCC CCTTGCCTTT TAACCTGCTG
    GGAGGGTCAG ACAAGGGATT TGGCATCTTT GTAGACAGCG TGGATCCAGG AAGCAAAGCA
    GCAGAGACTG GCTTAAAGCG AGGGGATCAG ATTTTGGAGG TAAATGGGCA GAACTTTGAA
    AACATTCAAC TTACCAAGGC TTTGGAAATC CTAAGGAACA ATACTCACTT GTCTCTCACT
    GTGAAAACAA ATTTATTTGT GTTTAAGGAA CTTCTGGCCA GAATGTTAGA GGAAAAGAGA
    AATAGTGCCC CACACTTGCC CAAAATTGGA GACATCAAAA AAGCCAGCCG CTATTCTATT
    CCAGACCTTG CCGTTGATGT TGAACAAGTT ATTGGACTTG AAAAAGTGAG CAAAAAGGGC
    AAAGCCAATA CTGTCGGAGG ACGAAACAAG CTGAAAAAGA TTCTTGACAA GACACGGATC
    AGTATCCTGC CTCAAAAGCC TTACAATGAT ATTGGAATCG GTCAGTCTCA AGATGACAGC
    ATTGTCGGTC TCCGACAGAC CAAGCACATG CCGGCTACAC TACCTGTCAG TGGGACATTA
    TCTTCCAGCA ATCCGGATTT ACTGCAATCC CATCACCGCA TCTTAGACTT TAATACTACA
    CCAGAACAAC CGGACCAGGT GCTGAGAGTT TTCAAGGCCG ATCAGCAGAG CCGTTACATA
    ATGATAAGCA AGGACACCAC CGCTAAGGAA GTGGTCATCC AGGCAATCAG AGAATTTGCA
    TTAACTGCGG TACCTGATGC CTACTCATTG TGCGAAGTGT CTGTCACCCC TGAAGGTGTC
    ATCAAACAAA GGCGGCTTCC TGATCAGCTT TCCAAGCTGG CTGACAGAAT ACAACTCAGT
    GGCAGGTATT ACCTAAAAAA CAACATGGAA ACTGAAACAC TATGCTCAGA TGAAGATGCC
    CAAGATCTTC TAAGGGAAAG TCAGATCTCT TTGCTACAGC TCAGCACCAT TGAAGTGGCA
    ACACAGCTGT CTATGAGAAA TTTTGAGCTA TTTAGGAACA TTGAGCCCAC CGAGTACATT
    GATGACTTGT TCAAACTGAA AATGAAAACT GGCTGCACCA ACCTCAAAAA CTTCGAAGAA
    GTCATTAACC AAGAGACCTT CTGGGTGGCC TCAGAAATTC TAAGGGAAAC TAACCAGCTA
    AAACGGATGA AAATCATCAA GCATTTTATC AAGATCGCAC TACATTGTAG AGAGTGCAAG
    AACTTCAACT CCATGTTTGC TATCATTAGT GGCTTAAATT TGGCACCAGT TTCAAGACTC
    CGAACAACTT GGGAAAAACT CCCAAGCAAA TATGAGAAAC TCTTTCAAGA CCTCCAGGAC
    CTCTTTGACC CTTCCAGGAA CATGGCTAAA TATCGGAATG TGCTGAATAG TCAGAACCTT
    CAGCCTCCCA TTATACCTTT ATTTCCTGTC ATTAAGAAAG ACCTCACCTT CCTTCATGAG
    GGCAATGACT CCAAAGTAGA CGGATTAGTT AACTTTGAGA AGCTTAGGAT GATTGCAAAA
    GAAATCCGCC ATGTGGGAAG AATGGCTTCA GTAAATATGG ATCCAGCTCT TATGTTCCGA
    ACCAGGAAGA AGAAGTGGAG GAGTCTGGGG TCTCTGAGTC AAGGCAGCAC CAATGCTACG
    GTCCTCGACG TCGCCCAGTC GGGGGGACAC AAAAAACGTG TGCGTCGCAG CTCTTTCCTT
    AATGCCAAGA AACTGTACGA AGATGCTCAG ATGGCTCGCA AGGTGAAGCA ATACTTATCT
    GATCTAGATC TTGAAATGGA TGAAGAGAAT CTTCAGACAC TTTCTGTACA GTGTGAACCA
    GCAACCAACA CATTGCCAAA AAATCCATGT GAGAAGAGAG CAGGGAAGCC AGACACATCG
    CCTGTGGCAC CACGGGCAGC AGCACAACAC AAACAGCAAC AGCAGCAGCA AACTAAGGTC
    AACCAGGCAT TGCAAGTTCC TGCTGTGGCT CTTTACCCAT CTCGTAAGAA AGTGCCAGTC
    AAGGATCTTC CACCCTTCGG CATCAACTCT CCCCAAGCTT TAAAGAAAAT TCTTTCGTTA
    TCAGAAGAAG GAAGTTTGGA AAGGCACAAG AAGCAAGCAG AGGACACCAT ATCAAATGCA
    TCCTCGCAGC TTTCATCCCC TCCGACATCC CCGCAGTGCT CTCCAAGGAA AGGTTACACA
    ATGGCAGCTA GTGGCACAGT GGATAACTTC TCTGATTCAG GGCACAGTGA AATCTCCTCC
    CGCTCCAGCA TTGTCAGTAA CTCTTCTTTT GACTGCGTGC ACTTGCATGA TGACCGGCGG
    CAGAGGCATT CCCTAAGCAT AGTGGAGACC AATCTTGGTG TGGGAAGGGC TGACAGAAGA
    GCCATTATAG ATGCAGACCA GTGCAGCCTA GGCTCTTACG CGCCAGTGGC AGAGACTCGG
    GGCCTCTATG CAGGTGCAAC AGTTCTCTCC TCCCCCAGCA TGGAGGAGCT TTCCCAGGAT
    CAGGGGGATC GAGCTTCTTT AGATGCAGCA GATAGTGGCC GTGGAAGCTG GACTTCATGT
    TCCAGCGGAT CCCATGATAA CATACAAACT ATCCCACATC AGAGAAGCTG GGAGACTCTT
    CCTTTTGGTC ATGTTCATTT TGATAACACA GCTGATGGGT CAGGGTTTTG GGCCACAGGT
    GGCCACATGG ACCACGGGAT ATTTCCTGAT CATAGCACAA AATATGGCAG ATACAGCCAA
    GGAAGAGAAC CTTTGGATCA AGCACAGTCA AGAGCCAGCT GGGCATCATC AACAGGCTAC
    TGGGGCGAGG ACTCAGAAGG TGATACCGGC ACCATTAAGA GAAGAGGTGG GAAAGATGTG
    GCCCTTGAAG CTGAAACCAG CAGTATAACG TCAATGACTC CTGAAGAAAC AAAGCCTCTC
    CCTGTACCCA CACATGTAGC TGTGACTGCA AGTTCTGCAA AAGGGCTCAT TGTGTATTGT
    GTTACCACAG CAAGGAAAGA AGGCCGGTAC AGAGAACCGC CGCCCACACC TCCAGGCTAT
    GTAGGAATCC CCATAGCTGA CTTTGCCGAT GGACACCCAC CTCTATCCAG AAAGCCACCA
    GATTACAATG TGGCCTTGCA GCGTTCCAGA ATGATGGCTC GGAGAAGTGA AAGTGAAGGG
    TCAGCAGCAA CCTCTCCGCC ATCACATAAT CAGACTTCTG GCAGGACTGC AAACAAACCC
    CAGTGGCATA AACCAAACGA GGCTGACCCA AGGCTTGCTC TCTATCAGGC ACAAGGGGTT
    ACAGAAAAAG ATGATGGTAA TTTTTTTGCC GATGATGAGC AAGTATCTGC CGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751661.1
    CDS73..4569
    Translation

    Target ORF information:

    RefSeq Version XM_031895801.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTCCCT 
    GCTGATTTCA CTAAACTGCA CATCACAGAC AGTCTACATC CACAGGTGAC GCACGTGTCC
    TCTAGTCATT CAGGATGTAG CATCACTAGC GATTCTGGGA GCAGCAGCTT GTCTGACATC
    TACCAGGCCA CAGAAAGTGA GCCAGGAGAT ATGGACCTGA GTGGGCTGCC AGAGACCGCT
    GTAGACTCAG AAGATGACGA TGATGAAGAG GATATTGAAA GAGCTTCTGA CCCTTTAATG
    AGCAGGGACA TTGTCAGAGA CTGTCTGGAG AAGGATCCAA TTGACAGGAC TGACGACGAT
    ATTGAACAAC TTCTGGAGTT CATGCACCAG TTGCCAGCAT TTGCCAACAT GACAATGTCT
    GTAAGACGAG AGCTCTGTGC AGTCATGGTG TTCGCTGTGG TCGAGCGGGC AGGAACTGTT
    GTACTAAATG ATGGGGAGGA GCTCGATTCC TGGTCAGTTA TTTTAAATGG CTCAGTAGAA
    GTGACATATC CTGATGGCAG GACAGAAATA CTTTGCATGG GGAACAGCTT TGGTGTGTCG
    CCAACCATGG AGAAGGAATA CATGAAGGGA GTCATGAGAA CCAAAGTGGA TGACTGCCAG
    TTTGTGTGCA TCGCCCAGCA AGATTACTGC CGCATCCTCA ATCAGGTGGA AAAGAACATG
    CAGAAAGTGG AAGAGGAGGG TGAGATTGTG ATGGTGAAAG AGCACAGAGA GCTTGATCGC
    ACCGGAACCA GGAAAGGTCA TATTGTCATC AAGGGAACAT CGGAACGCCT AACCATGCAT
    CTTGTGGAAG AACATTCTGT GGTTGACCCA ACATATATTG AAGATTTTCT CCTCACTTAC
    AGAACTTTCC TTTCTAGTCC GATGGAAGTG GGAAAGAAAC TACTGGAGTG GTTTAATGAC
    CCTAGTCTCA GGGACAAGGT TACACGAGTA GTGTTGCTCT GGGTAAATAA TCACTTTAAT
    GACTTTGAAG GGGACCCAGC CATGACTCGA TTTTTGGAAG AATTTGAGAA CAATTTGGAA
    CGTGAGAAAA TGGGTGGCCA CCTAAGGCTA TTAAATATTG CATGTGCTGC CAAAGCAAAA
    AGAAGATTGA TAACTCTAAC AAAGCCCTCC CGAGAAGCCC CCTTGCCTTT TAACCTGCTG
    GGAGGGTCAG ACAAGGGATT TGGCATCTTT GTAGACAGCG TGGATCCAGG AAGCAAAGCA
    GCAGAGACTG GCTTAAAGCG AGGGGATCAG ATTTTGGAGG TAAATGGGCA GAACTTTGAA
    AACATTCAAC TTACCAAGGC TTTGGAAATC CTAAGGAACA ATACTCACTT GTCTCTCACT
    GTGAAAACAA ATTTATTTGT GTTTAAGGAA CTTCTGGCCA GAATGTTAGA GGAAAAGAGA
    AATAGTGCCC CACACTTGCC CAAAATTGGA GACATCAAAA AAGCCAGCCG CTATTCTATT
    CCAGACCTTG CCGTTGATGT TGAACAAGTT ATTGGACTTG AAAAAGTGAG CAAAAAGGGC
    AAAGCCAATA CTGTCGGAGG ACGAAACAAG CTGAAAAAGA TTCTTGACAA GACACGGATC
    AGTATCCTGC CTCAAAAGCC TTACAATGAT ATTGGAATCG GTCAGTCTCA AGATGACAGC
    ATTGTCGGTC TCCGACAGAC CAAGCACATG CCGGCTACAC TACCTGTCAG TGGGACATTA
    TCTTCCAGCA ATCCGGATTT ACTGCAATCC CATCACCGCA TCTTAGACTT TAATACTACA
    CCAGAACAAC CGGACCAGGT GCTGAGAGTT TTCAAGGCCG ATCAGCAGAG CCGTTACATA
    ATGATAAGCA AGGACACCAC CGCTAAGGAA GTGGTCATCC AGGCAATCAG AGAATTTGCA
    TTAACTGCGG TACCTGATGC CTACTCATTG TGCGAAGTGT CTGTCACCCC TGAAGGTGTC
    ATCAAACAAA GGCGGCTTCC TGATCAGCTT TCCAAGCTGG CTGACAGAAT ACAACTCAGT
    GGCAGGTATT ACCTAAAAAA CAACATGGAA ACTGAAACAC TATGCTCAGA TGAAGATGCC
    CAAGATCTTC TAAGGGAAAG TCAGATCTCT TTGCTACAGC TCAGCACCAT TGAAGTGGCA
    ACACAGCTGT CTATGAGAAA TTTTGAGCTA TTTAGGAACA TTGAGCCCAC CGAGTACATT
    GATGACTTGT TCAAACTGAA AATGAAAACT GGCTGCACCA ACCTCAAAAA CTTCGAAGAA
    GTCATTAACC AAGAGACCTT CTGGGTGGCC TCAGAAATTC TAAGGGAAAC TAACCAGCTA
    AAACGGATGA AAATCATCAA GCATTTTATC AAGATCGCAC TACATTGTAG AGAGTGCAAG
    AACTTCAACT CCATGTTTGC TATCATTAGT GGCTTAAATT TGGCACCAGT TTCAAGACTC
    CGAACAACTT GGGAAAAACT CCCAAGCAAA TATGAGAAAC TCTTTCAAGA CCTCCAGGAC
    CTCTTTGACC CTTCCAGGAA CATGGCTAAA TATCGGAATG TGCTGAATAG TCAGAACCTT
    CAGCCTCCCA TTATACCTTT ATTTCCTGTC ATTAAGAAAG ACCTCACCTT CCTTCATGAG
    GGCAATGACT CCAAAGTAGA CGGATTAGTT AACTTTGAGA AGCTTAGGAT GATTGCAAAA
    GAAATCCGCC ATGTGGGAAG AATGGCTTCA GTAAATATGG ATCCAGCTCT TATGTTCCGA
    ACCAGGAAGA AGAAGTGGAG GAGTCTGGGG TCTCTGAGTC AAGGCAGCAC CAATGCTACG
    GTCCTCGACG TCGCCCAGTC GGGGGGACAC AAAAAACGTG TGCGTCGCAG CTCTTTCCTT
    AATGCCAAGA AACTGTACGA AGATGCTCAG ATGGCTCGCA AGGTGAAGCA ATACTTATCT
    GATCTAGATC TTGAAATGGA TGAAGAGAAT CTTCAGACAC TTTCTGTACA GTGTGAACCA
    GCAACCAACA CATTGCCAAA AAATCCATGT GAGAAGAGAG CAGGGAAGCC AGACACATCG
    CCTGTGGCAC CACGGGCAGC AGCACAACAC AAACAGCAAC AGCAGCAGCA AACTAAGGTC
    AACCAGGCAT TGCAAGTTCC TGCTGTGGCT CTTTACCCAT CTCGTAAGAA AGTGCCAGTC
    AAGGATCTTC CACCCTTCGG CATCAACTCT CCCCAAGCTT TAAAGAAAAT TCTTTCGTTA
    TCAGAAGAAG GAAGTTTGGA AAGGCACAAG AAGCAAGCAG AGGACACCAT ATCAAATGCA
    TCCTCGCAGC TTTCATCCCC TCCGACATCC CCGCAGTGCT CTCCAAGGAA AGGTTACACA
    ATGGCAGCTA GTGGCACAGT GGATAACTTC TCTGATTCAG GGCACAGTGA AATCTCCTCC
    CGCTCCAGCA TTGTCAGTAA CTCTTCTTTT GACTGCGTGC ACTTGCATGA TGACCGGCGG
    CAGAGGCATT CCCTAAGCAT AGTGGAGACC AATCTTGGTG TGGGAAGGGC TGACAGAAGA
    GCCATTATAG ATGCAGACCA GTGCAGCCTA GGCTCTTACG CGCCAGTGGC AGAGACTCGG
    GGCCTCTATG CAGGTGCAAC AGTTCTCTCC TCCCCCAGCA TGGAGGAGCT TTCCCAGGAT
    CAGGGGGATC GAGCTTCTTT AGATGCAGCA GATAGTGGCC GTGGAAGCTG GACTTCATGT
    TCCAGCGGAT CCCATGATAA CATACAAACT ATCCCACATC AGAGAAGCTG GGAGACTCTT
    CCTTTTGGTC ATGTTCATTT TGATAACACA GCTGATGGGT CAGGGTTTTG GGCCACAGGT
    GGCCACATGG ACCACGGGAT ATTTCCTGAT CATAGCACAA AATATGGCAG ATACAGCCAA
    GGAAGAGAAC CTTTGGATCA AGCACAGTCA AGAGCCAGCT GGGCATCATC AACAGGCTAC
    TGGGGCGAGG ACTCAGAAGG TGATACCGGC ACCATTAAGA GAAGAGGTGG GAAAGATGTG
    GCCCTTGAAG CTGAAACCAG CAGTATAACG TCAATGACTC CTGAAGAAAC AAAGCCTCTC
    CCTGTACCCA CACATGTAGC TGTGACTGCA AGTTCTGCAA AAGGGCTCAT TGTGTATTGT
    GTTACCACAG CAAGGAAAGA AGGCCGGTAC AGAGAACCGC CGCCCACACC TCCAGGCTAT
    GTAGGAATCC CCATAGCTGA CTTTGCCGAT GGACACCCAC CTCTATCCAG AAAGCCACCA
    GATTACAATG TGGCCTTGCA GCGTTCCAGA ATGATGGCTC GGAGAAGTGA AAGTGAAGGG
    TCAGCAGCAA CCTCTCCGCC ATCACATAAT CAGACTTCTG GCAGGACTGC AAACAAACCC
    CAGTGGCATA AACCAAACGA GGCTGACCCA AGGCTTGCTC TCTATCAGGC ACAAGGGGTT
    ACAGAAAAAG ATGATGGTAA TTTTTTTGCC GATGATGAGC AAGTATCTGC CGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41692
    Clone ID Related Accession (Same CDS sequence) XM_018091100.1
    Accession Version XM_018091100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG TTTGTGTGCA TCGCCCAGCA AGATTACTGC
    CGCATCCTCA ATCAGGTGGA AAAGAACATG CAGAAAGTGG AAGAGGAGGG TGAGATTGTG
    ATGGTGAAAG AGCACAGAGA GCTTGATCGC ACCGGAACCA GGAAAGGTCA TATTGTCATC
    AAGGGAACAT CGGAACGCCT AACCATGCAT CTTGTGGAAG AACATTCTGT GGTTGACCCA
    ACATATATTG AAGATTTTCT CCTCACTTAC AGAACTTTCC TTTCTAGTCC GATGGAAGTG
    GGAAAGAAAC TACTGGAGTG GTTTAATGAC CCTAGTCTCA GGGACAAGGT TACACGAGTA
    GTTTTGCTCT GGGTAAATAA TCACTTTAAT GACTTTGAAG GGGACCCAGC CATGACTCGA
    TTTTTGGAAG AATTTGAGAA CAATTTGGAA CGTGAGAAAA TGGGTGGCCA CCTAAGGCTA
    TTAAATATTG CATGTGCTGC CAAAGCAAAA AGAAGATTGA TAACTCTAAC AAAGCCCTCC
    CGAGAAGCCC CCTTGCCTTT TAACCTGCTG GGAGGGTCAG ACAAGGGATT TGGCATCTTT
    GTAGACAGCG TGGATCCAGG AAGCAAAGCA GCAGAGACTG GCTTAAAGCG AGGGGATCAG
    ATTTTGGAGG TAAATGGGCA GAACTTTGAA AACATTCAAC TTACCAAGGC TTTGGAAATC
    CTAAGGAACA ATACTCACTT GTCTCTCACT GTGAAAACAA ATTTATTTGT GTTTAAGGAA
    CTTCTGGCCA GAATGTTAGA GGAAAAGAGA AATAGTGCCC CACACTTGCC CAAAATTGGA
    GACATCAAAA AAGCCAGCCG CTATTCTATT CCAGACCTTG CCGTTGATGT TGAACAAGTT
    ATTGGACTTG AAAAAGTGAG CAAAAAGGGC AAAGCCAATA CTGTCGGAGG ACGAAACAAG
    CTGAAAAAGA TTCTTGACAA GACACGGATC AGTATCCTGC CTCAAAAGCC TTACAATGAT
    ATTGGAATCG GTCAGTCTCA AGATGACAGC ATTGTCGGTC TCCGACAGAC CAAGCACATG
    CCGGCTACAC TACCTGTCAG TGGGACATTA TCTTCCAGCA ATCCGGATTT ACTGCAGTCC
    CATCACCGCA TCTTAGACTT TAATACTACA CCAGAACAAC CGGACCAGGT GCTGAGAGTT
    TTCAAGGCCG ATCAGCAGAG CCGTTACATA ATGATAAGCA AGGACACCAC CGCTAAGGAA
    GTGGTCATCC AGGCAATCAG AGAATTTGCA TTAACTGCGG TACCTGATGC CTACTCATTG
    TGTGAAGTGT CTGTCACCCC TGAAGGTGTC ATCAAACAAA GGCGGCTTCC TGATCAGCTT
    TCCAAGCTGG CTGACAGAAT ACAACTCAGT GGCAGGTATT ACCTAAAAAA CAACATGGAA
    ACTGAAACAC TATGCTCAGA TGAAGATGCC CAAGATCTTC TAAGGGAAAG TCAGATCTCT
    TTGCTACAGC TCAGCACCAT TGAAGTGGCA ACACAGCTGT CTATGAGAAA TTTTGAGCTA
    TTTAGGAACA TTGAGCCCAC CGAGTACATT GATGACTTGT TCAAACTGAA AATGAAAACT
    GGCTGCACCA ACCTCAAAAA CTTCGAAGAA GTCATTAACC AAGAGACCTT CTGGGTAGCC
    TCAGAAATTC TAAGGGAAAC TAACCAGCTA AAACGGATGA AGATCATCAA GCATTTTATC
    AAGATCGCAC TACATTGTAG AGAGTGCAAG AACTTCAACT CCATGTTTGC TATCATTAGT
    GGCTTAAATT TGGCACCAGT TTCAAGACTC CGAACAACTT GGGAAAAACT CCCAAGCAAA
    TATGAGAAAC TCTTTCAAGA CCTCCAGGAC CTCTTTGACC CTTCCAGGAA CATGGCTAAA
    TATCGGAATG TGCTGAATAG TCAGAACCTT CAGCCTCCCA TTATACCTTT ATTTCCTGTC
    ATTAAGAAAG ACCTCACCTT CCTTCATGAG GGCAATGACT CCAAAGTAGA CGGATTAGTT
    AACTTTGAGA AGCTTAGGAT GATTGCAAAA GAAATCCGCC ATGTGGGAAG AATGGCTTCA
    GTAAATATGG ATCCAGCTCT TATGTTCCGA ACCAGGAAGA AGAAGTGGAG GAGTCTGGGG
    TCTCTGAGTC AAGGCAGCAC CAATGCTACG GTCCTCGACG TCGCCCAGTC GGGGGGACAC
    AAAAAACGTG TGCGTCGCAG CTCTTTCCTT AATGCCAAGA AACTGTACGA AGATGCTCAG
    ATGGCTCGCA AGGTGAAGCA ATACTTATCT GATCTAGATC TTGAAATGGA TGAAGAGAAT
    CTTCAGACAC TTTCTGTACA GTGTGAACCA GCAACCAACA CATTGCCAAA AAATCCATGT
    GAGAAGAGAG CAGGGAAGCC AGACACATCG CCTGTGGCAC CACGGGCAGC AGCACAACAC
    AAACAGCAAC AGCAGCAGCA AACTAAGGTC AACCAGGCAT TGCAAGTTCC TGCTGTGGCT
    CTTTACCCAT CTCGTAAGAA AGTGCCAGTC AAGGATCTTC CACCCTTCGG CATCAACTCT
    CCCCAAGCTT TAAAGAAAAT TCTTTCGTTA TCAGAAGAAG GAAGTTTGGA AAGGCACAAG
    AAGCAAGCAG AGGACACCAT ATCAAATGCA TCCTCGCAGC TTTCATCCCC TCCGACATCC
    CCGCAGTGCT CTCCAAGGAA AGGTTACACA ATGGCAGCTA GTGGCACAGT GGATAACTTC
    TCTGATTCAG GGCACAGTGA AATCTCCTCC CGCTCCAGCA TTGTCAGTAA CTCTTCTTTT
    GACTGCGTGC ACTTGCACGA TGACCGGCGG CAGAGGCATT CCCTAAGCAT AGTGGAGACC
    AATCTTGGTG TGGGAAGGGC TGACAGAAGA GCCATTATAG ATGCAGACCA GTGCAGCCTA
    GGCTCTTACG CGCCAGTGGC AGAGACTCGG GGCCTCTATG CAGGTGCAAC AGTTCTCTCC
    TCCCCCAGCA TGGAGGAGCT TTCCCAGGAT CAGGGGGATC GAGCTTCTTT AGATGCAGCA
    GATAGTGGCC GTGGAAGCTG GACTTCATGT TCCAGCGGAT CCCATGATAA CATACAAACT
    ATCCCACATC AGAGAAGCTG GGAGACTCTT CCTTTTGGTC ATGTTCATTT TGATAACACA
    GCTGATGGGT CAGGGTTTTG GGCCACAGGT GGCCACATGG ACCACGGGAT ATTTCCTGAT
    CATAGCACAA AATATGGCAG ATACAGCCAA GGAAGAGAAC CTTTGGATCA AGCACAGTCA
    AGAGCCAGCT GGGCATCATC AACAGGCTAC TGGGGCGAGG ACTCAGAAGG TGATACCGGC
    ACCATTAAGA GAAGAGGTGG GAAAGATGTG GCCCTTGAAG CTGAAACCAG CAGTATAACA
    TCAATGACTC CTGAAGAAAC AAAGCCTCTC CCTGTACCCA CACATGTAGC TGTGACTGCA
    AGTTCTGCAA AAGGGCTCAT TGTGTATTGT GTTACCACAG CAAGGAAAGA AGGCCGGTAC
    AGAGAACCGC CGCCCACACC TCCAGGCTAT GTAGGAATCC CCATAGCTGA CTTTGCCGAT
    GGACACCCAC CTCTATCCAG AAAGCCACCA GATTACAATG TGGCCTTGCA GCGTTCCAGA
    ATGATGGCTC GGAGAAGTGA AAGTGAAGGG TCAGCAGCAA CCTCTCCGCC ATCACATAAT
    CAGACTTCTG GCAGGACTGC AAACAAACCC CAGTGGCATA AACCAAACGA GGCTGACCCA
    AGGCTTGCTC TCTATCAGGC ACAAGGGGTT ACAGAAAAAG ATGATGGTAA TTTTTTTGCC
    GATGATGAGC AAGTATCTGC CGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946589.1
    CDS470..5296
    Translation

    Target ORF information:

    RefSeq Version XM_018091100.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG TTTGTGTGCA TCGCCCAGCA AGATTACTGC
    CGCATCCTCA ATCAGGTGGA AAAGAACATG CAGAAAGTGG AAGAGGAGGG TGAGATTGTG
    ATGGTGAAAG AGCACAGAGA GCTTGATCGC ACCGGAACCA GGAAAGGTCA TATTGTCATC
    AAGGGAACAT CGGAACGCCT AACCATGCAT CTTGTGGAAG AACATTCTGT GGTTGACCCA
    ACATATATTG AAGATTTTCT CCTCACTTAC AGAACTTTCC TTTCTAGTCC GATGGAAGTG
    GGAAAGAAAC TACTGGAGTG GTTTAATGAC CCTAGTCTCA GGGACAAGGT TACACGAGTA
    GTTTTGCTCT GGGTAAATAA TCACTTTAAT GACTTTGAAG GGGACCCAGC CATGACTCGA
    TTTTTGGAAG AATTTGAGAA CAATTTGGAA CGTGAGAAAA TGGGTGGCCA CCTAAGGCTA
    TTAAATATTG CATGTGCTGC CAAAGCAAAA AGAAGATTGA TAACTCTAAC AAAGCCCTCC
    CGAGAAGCCC CCTTGCCTTT TAACCTGCTG GGAGGGTCAG ACAAGGGATT TGGCATCTTT
    GTAGACAGCG TGGATCCAGG AAGCAAAGCA GCAGAGACTG GCTTAAAGCG AGGGGATCAG
    ATTTTGGAGG TAAATGGGCA GAACTTTGAA AACATTCAAC TTACCAAGGC TTTGGAAATC
    CTAAGGAACA ATACTCACTT GTCTCTCACT GTGAAAACAA ATTTATTTGT GTTTAAGGAA
    CTTCTGGCCA GAATGTTAGA GGAAAAGAGA AATAGTGCCC CACACTTGCC CAAAATTGGA
    GACATCAAAA AAGCCAGCCG CTATTCTATT CCAGACCTTG CCGTTGATGT TGAACAAGTT
    ATTGGACTTG AAAAAGTGAG CAAAAAGGGC AAAGCCAATA CTGTCGGAGG ACGAAACAAG
    CTGAAAAAGA TTCTTGACAA GACACGGATC AGTATCCTGC CTCAAAAGCC TTACAATGAT
    ATTGGAATCG GTCAGTCTCA AGATGACAGC ATTGTCGGTC TCCGACAGAC CAAGCACATG
    CCGGCTACAC TACCTGTCAG TGGGACATTA TCTTCCAGCA ATCCGGATTT ACTGCAGTCC
    CATCACCGCA TCTTAGACTT TAATACTACA CCAGAACAAC CGGACCAGGT GCTGAGAGTT
    TTCAAGGCCG ATCAGCAGAG CCGTTACATA ATGATAAGCA AGGACACCAC CGCTAAGGAA
    GTGGTCATCC AGGCAATCAG AGAATTTGCA TTAACTGCGG TACCTGATGC CTACTCATTG
    TGTGAAGTGT CTGTCACCCC TGAAGGTGTC ATCAAACAAA GGCGGCTTCC TGATCAGCTT
    TCCAAGCTGG CTGACAGAAT ACAACTCAGT GGCAGGTATT ACCTAAAAAA CAACATGGAA
    ACTGAAACAC TATGCTCAGA TGAAGATGCC CAAGATCTTC TAAGGGAAAG TCAGATCTCT
    TTGCTACAGC TCAGCACCAT TGAAGTGGCA ACACAGCTGT CTATGAGAAA TTTTGAGCTA
    TTTAGGAACA TTGAGCCCAC CGAGTACATT GATGACTTGT TCAAACTGAA AATGAAAACT
    GGCTGCACCA ACCTCAAAAA CTTCGAAGAA GTCATTAACC AAGAGACCTT CTGGGTAGCC
    TCAGAAATTC TAAGGGAAAC TAACCAGCTA AAACGGATGA AGATCATCAA GCATTTTATC
    AAGATCGCAC TACATTGTAG AGAGTGCAAG AACTTCAACT CCATGTTTGC TATCATTAGT
    GGCTTAAATT TGGCACCAGT TTCAAGACTC CGAACAACTT GGGAAAAACT CCCAAGCAAA
    TATGAGAAAC TCTTTCAAGA CCTCCAGGAC CTCTTTGACC CTTCCAGGAA CATGGCTAAA
    TATCGGAATG TGCTGAATAG TCAGAACCTT CAGCCTCCCA TTATACCTTT ATTTCCTGTC
    ATTAAGAAAG ACCTCACCTT CCTTCATGAG GGCAATGACT CCAAAGTAGA CGGATTAGTT
    AACTTTGAGA AGCTTAGGAT GATTGCAAAA GAAATCCGCC ATGTGGGAAG AATGGCTTCA
    GTAAATATGG ATCCAGCTCT TATGTTCCGA ACCAGGAAGA AGAAGTGGAG GAGTCTGGGG
    TCTCTGAGTC AAGGCAGCAC CAATGCTACG GTCCTCGACG TCGCCCAGTC GGGGGGACAC
    AAAAAACGTG TGCGTCGCAG CTCTTTCCTT AATGCCAAGA AACTGTACGA AGATGCTCAG
    ATGGCTCGCA AGGTGAAGCA ATACTTATCT GATCTAGATC TTGAAATGGA TGAAGAGAAT
    CTTCAGACAC TTTCTGTACA GTGTGAACCA GCAACCAACA CATTGCCAAA AAATCCATGT
    GAGAAGAGAG CAGGGAAGCC AGACACATCG CCTGTGGCAC CACGGGCAGC AGCACAACAC
    AAACAGCAAC AGCAGCAGCA AACTAAGGTC AACCAGGCAT TGCAAGTTCC TGCTGTGGCT
    CTTTACCCAT CTCGTAAGAA AGTGCCAGTC AAGGATCTTC CACCCTTCGG CATCAACTCT
    CCCCAAGCTT TAAAGAAAAT TCTTTCGTTA TCAGAAGAAG GAAGTTTGGA AAGGCACAAG
    AAGCAAGCAG AGGACACCAT ATCAAATGCA TCCTCGCAGC TTTCATCCCC TCCGACATCC
    CCGCAGTGCT CTCCAAGGAA AGGTTACACA ATGGCAGCTA GTGGCACAGT GGATAACTTC
    TCTGATTCAG GGCACAGTGA AATCTCCTCC CGCTCCAGCA TTGTCAGTAA CTCTTCTTTT
    GACTGCGTGC ACTTGCACGA TGACCGGCGG CAGAGGCATT CCCTAAGCAT AGTGGAGACC
    AATCTTGGTG TGGGAAGGGC TGACAGAAGA GCCATTATAG ATGCAGACCA GTGCAGCCTA
    GGCTCTTACG CGCCAGTGGC AGAGACTCGG GGCCTCTATG CAGGTGCAAC AGTTCTCTCC
    TCCCCCAGCA TGGAGGAGCT TTCCCAGGAT CAGGGGGATC GAGCTTCTTT AGATGCAGCA
    GATAGTGGCC GTGGAAGCTG GACTTCATGT TCCAGCGGAT CCCATGATAA CATACAAACT
    ATCCCACATC AGAGAAGCTG GGAGACTCTT CCTTTTGGTC ATGTTCATTT TGATAACACA
    GCTGATGGGT CAGGGTTTTG GGCCACAGGT GGCCACATGG ACCACGGGAT ATTTCCTGAT
    CATAGCACAA AATATGGCAG ATACAGCCAA GGAAGAGAAC CTTTGGATCA AGCACAGTCA
    AGAGCCAGCT GGGCATCATC AACAGGCTAC TGGGGCGAGG ACTCAGAAGG TGATACCGGC
    ACCATTAAGA GAAGAGGTGG GAAAGATGTG GCCCTTGAAG CTGAAACCAG CAGTATAACA
    TCAATGACTC CTGAAGAAAC AAAGCCTCTC CCTGTACCCA CACATGTAGC TGTGACTGCA
    AGTTCTGCAA AAGGGCTCAT TGTGTATTGT GTTACCACAG CAAGGAAAGA AGGCCGGTAC
    AGAGAACCGC CGCCCACACC TCCAGGCTAT GTAGGAATCC CCATAGCTGA CTTTGCCGAT
    GGACACCCAC CTCTATCCAG AAAGCCACCA GATTACAATG TGGCCTTGCA GCGTTCCAGA
    ATGATGGCTC GGAGAAGTGA AAGTGAAGGG TCAGCAGCAA CCTCTCCGCC ATCACATAAT
    CAGACTTCTG GCAGGACTGC AAACAAACCC CAGTGGCATA AACCAAACGA GGCTGACCCA
    AGGCTTGCTC TCTATCAGGC ACAAGGGGTT ACAGAAAAAG ATGATGGTAA TTTTTTTGCC
    GATGATGAGC AAGTATCTGC CGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47591
    Clone ID Related Accession (Same CDS sequence) XM_012956489.3
    Accession Version XM_012956489.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956489.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGGTAAT TTTTTTGCCG ATGATGAGCA AGTATCTGCC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811943.1
    CDS470..5455
    Translation

    Target ORF information:

    RefSeq Version XM_012956489.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956489.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGGTAAT TTTTTTGCCG ATGATGAGCA AGTATCTGCC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47592
    Clone ID Related Accession (Same CDS sequence) XM_012956494.2
    Accession Version XM_012956494.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGCCGAT GATGAGCAAG TATCTGCCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811948.1
    CDS354..5327
    Translation

    Target ORF information:

    RefSeq Version XM_012956494.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956494.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGCCGAT GATGAGCAAG TATCTGCCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47593
    Clone ID Related Accession (Same CDS sequence) XM_012956500.2
    Accession Version XM_012956500.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811954.1
    CDS354..5321
    Translation

    Target ORF information:

    RefSeq Version XM_012956500.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47594
    Clone ID Related Accession (Same CDS sequence) XM_012956496.3
    Accession Version XM_012956496.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTGTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAATC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGCGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTGGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAAATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCATG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC GTCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGTGTATTG TGTTACCACA
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811950.1
    CDS470..5437
    Translation

    Target ORF information:

    RefSeq Version XM_012956496.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956496.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTGTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAATC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGCGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTGGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAAATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCATG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC GTCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGTGTATTG TGTTACCACA
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47595
    Clone ID Related Accession (Same CDS sequence) XM_012956506.2
    Accession Version XM_012956506.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGTCTCT GAGTCAAGGC AGCACCAATG CTACGGTCCT CGACGTCGCC
    CAGTCGGGGG GACACAAAAA ACGTGTGCGT CGCAGCTCTT TCCTTAATGC CAAGAAACTG
    TACGAAGATG CTCAGATGGC TCGCAAGGTG AAGCAATACT TATCTGATCT AGATCTTGAA
    ATGGATGAAG AGAATCTTCA GACACTTTCT GTACAGTGTG AACCAGCAAC CAACACATTG
    CCAAAAAATC CATGTGAGAA GAGAGCAGGG AAGCCAGACA CATCGCCTGT GGCACCACGG
    GCAGCAGCAC AACACAAACA GCAACAGCAG CAGCAAACTA AGGTCAACCA GGCATTGCAA
    GTTCCTGCTG TGGCTCTTTA CCCATCTCGT AAGAAAGTGC CAGTCAAGGA TCTTCCACCC
    TTCGGCATCA ACTCTCCCCA AGCTTTAAAG AAAATTCTTT CGTTATCAGA AGAAGGAAGT
    TTGGAAAGGC ACAAGAAGCA AGCAGAGGAC ACCATATCAA ATGCATCCTC GCAGCTTTCA
    TCCCCTCCGA CATCCCCGCA GTGCTCTCCA AGGAAAGGTT ACACAATGGC AGCTAGTGGC
    ACAGTGGATA ACTTCTCTGA TTCAGGGCAC AGTGAAATCT CCTCCCGCTC CAGCATTGTC
    AGTAACTCTT CTTTTGACTG CGTGCACTTG CATGATGACC GGCGGCAGAG GCATTCCCTA
    AGCATAGTGG AGACCAATCT TGGTGTGGGA AGGGCTGACA GAAGAGCCAT TATAGATGCA
    GACCAGTGCA GCCTAGGCTC TTACGCGCCA GTGGCAGAGA CTCGGGGCCT CTATGCAGGT
    GCAACAGTTC TCTCCTCCCC CAGCATGGAG GAGCTTTCCC AGGATCAGGG GGATCGAGCT
    TCTTTAGATG CAGCAGATAG TGGCCGTGGA AGCTGGACTT CATGTTCCAG CGGATCCCAT
    GATAACATAC AAACTATCCC ACATCAGAGA AGCTGGGAGA CTCTTCCTTT TGGTCATGTT
    CATTTTGATA ACACAGCTGA TGGGTCAGGG TTTTGGGCCA CAGGTGGCCA CATGGACCAC
    GGGATATTTC CTGATCATAG CACAAAATAT GGCAGATACA GCCAAGGAAG AGAACCTTTG
    GATCAAGCAC AGTCAAGAGC CAGCTGGGCA TCATCAACAG GCTACTGGGG CGAGGACTCA
    GAAGGTGATA CCGGCACCAT TAAGAGAAGA GGTGGGAAAG ATGTGGCCCT TGAAGCTGAA
    ACCAGCAGTA TAACGTCAAT GACTCCTGAA GAAACAAAGC CTCTCCCTGT ACCCACACAT
    GTAGCTGTGA CTGCAAGTTC TGCAAAAGGG CTCATTGTGT ATTGTGTTAC CACAGCAAGG
    AAAGAAGGCC GGTACAGAGA ACCGCCGCCC ACACCTCCAG GCTATGTAGG AATCCCCATA
    GCTGACTTTG CCGATGGACA CCCACCTCTA TCCAGAAAGC CACCAGATTA CAATGTGGCC
    TTGCAGCGTT CCAGAATGAT GGCTCGGAGA AGTGAAAGTG AAGGGTCAGC AGCAACCTCT
    CCGCCATCAC ATAATCAGAC TTCTGGCAGG ACTGCAAACA AACCCCAGTG GCATAAACCA
    AACGAGGCTG ACCCAAGGCT TGCTCTCTAT CAGGCACAAG GGGTTACAGA AAAAGATGAT
    GGTAATTTTT TTGCCGATGA TGAGCAAGTA TCTGCCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811960.1
    CDS354..5315
    Translation

    Target ORF information:

    RefSeq Version XM_012956506.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGTCTCT GAGTCAAGGC AGCACCAATG CTACGGTCCT CGACGTCGCC
    CAGTCGGGGG GACACAAAAA ACGTGTGCGT CGCAGCTCTT TCCTTAATGC CAAGAAACTG
    TACGAAGATG CTCAGATGGC TCGCAAGGTG AAGCAATACT TATCTGATCT AGATCTTGAA
    ATGGATGAAG AGAATCTTCA GACACTTTCT GTACAGTGTG AACCAGCAAC CAACACATTG
    CCAAAAAATC CATGTGAGAA GAGAGCAGGG AAGCCAGACA CATCGCCTGT GGCACCACGG
    GCAGCAGCAC AACACAAACA GCAACAGCAG CAGCAAACTA AGGTCAACCA GGCATTGCAA
    GTTCCTGCTG TGGCTCTTTA CCCATCTCGT AAGAAAGTGC CAGTCAAGGA TCTTCCACCC
    TTCGGCATCA ACTCTCCCCA AGCTTTAAAG AAAATTCTTT CGTTATCAGA AGAAGGAAGT
    TTGGAAAGGC ACAAGAAGCA AGCAGAGGAC ACCATATCAA ATGCATCCTC GCAGCTTTCA
    TCCCCTCCGA CATCCCCGCA GTGCTCTCCA AGGAAAGGTT ACACAATGGC AGCTAGTGGC
    ACAGTGGATA ACTTCTCTGA TTCAGGGCAC AGTGAAATCT CCTCCCGCTC CAGCATTGTC
    AGTAACTCTT CTTTTGACTG CGTGCACTTG CATGATGACC GGCGGCAGAG GCATTCCCTA
    AGCATAGTGG AGACCAATCT TGGTGTGGGA AGGGCTGACA GAAGAGCCAT TATAGATGCA
    GACCAGTGCA GCCTAGGCTC TTACGCGCCA GTGGCAGAGA CTCGGGGCCT CTATGCAGGT
    GCAACAGTTC TCTCCTCCCC CAGCATGGAG GAGCTTTCCC AGGATCAGGG GGATCGAGCT
    TCTTTAGATG CAGCAGATAG TGGCCGTGGA AGCTGGACTT CATGTTCCAG CGGATCCCAT
    GATAACATAC AAACTATCCC ACATCAGAGA AGCTGGGAGA CTCTTCCTTT TGGTCATGTT
    CATTTTGATA ACACAGCTGA TGGGTCAGGG TTTTGGGCCA CAGGTGGCCA CATGGACCAC
    GGGATATTTC CTGATCATAG CACAAAATAT GGCAGATACA GCCAAGGAAG AGAACCTTTG
    GATCAAGCAC AGTCAAGAGC CAGCTGGGCA TCATCAACAG GCTACTGGGG CGAGGACTCA
    GAAGGTGATA CCGGCACCAT TAAGAGAAGA GGTGGGAAAG ATGTGGCCCT TGAAGCTGAA
    ACCAGCAGTA TAACGTCAAT GACTCCTGAA GAAACAAAGC CTCTCCCTGT ACCCACACAT
    GTAGCTGTGA CTGCAAGTTC TGCAAAAGGG CTCATTGTGT ATTGTGTTAC CACAGCAAGG
    AAAGAAGGCC GGTACAGAGA ACCGCCGCCC ACACCTCCAG GCTATGTAGG AATCCCCATA
    GCTGACTTTG CCGATGGACA CCCACCTCTA TCCAGAAAGC CACCAGATTA CAATGTGGCC
    TTGCAGCGTT CCAGAATGAT GGCTCGGAGA AGTGAAAGTG AAGGGTCAGC AGCAACCTCT
    CCGCCATCAC ATAATCAGAC TTCTGGCAGG ACTGCAAACA AACCCCAGTG GCATAAACCA
    AACGAGGCTG ACCCAAGGCT TGCTCTCTAT CAGGCACAAG GGGTTACAGA AAAAGATGAT
    GGTAATTTTT TTGCCGATGA TGAGCAAGTA TCTGCCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47596
    Clone ID Related Accession (Same CDS sequence) XM_012956508.2
    Accession Version XM_012956508.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGCCG ATGATGAGCA AGTATCTGCC GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811962.1
    CDS354..5309
    Translation

    Target ORF information:

    RefSeq Version XM_012956508.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956508.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TGTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAATCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GCGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTGGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA AATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCATGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACGT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGCCG ATGATGAGCA AGTATCTGCC GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47597
    Clone ID Related Accession (Same CDS sequence) XM_012956514.3
    Accession Version XM_012956514.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956514.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAGCGCCA GGCATCTCAC 
    CGGCAGTCTC GCCGGAGGTT CCGCAAGATC AATCAGAAAG GCGAGAGGCA AACCATTATT
    GATACAGTGG AATCCTATCC TGTTGGAAAA CCACCTTTGC CAAGAGGGTA TCATACGGAA
    GGCTCTAAGC CTCAGCTCCC TGCTGATTTC ACTAAACTGC ACATCACAGA CAGTCTACAT
    CCACAGGTGA CGCACGTGTC CTCTAGTCAT TCAGGATGTA GCATCACTAG CGATTCTGGG
    AGCAGCAGCT TGTCTGACAT CTACCAGGCC ACAGAAAGTG AGCCAGGAGA TATGGACCTG
    AGTGGGCTGC CAGAGACCGC TGTAGACTCA GAAGATGACG ATGATGAAGA GGATATTGAA
    AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC ATTGTCAGAG ACTGTCTGGA GAAGGATCCA
    ATTGACAGGA CTGACGACGA TATTGAACAA CTTCTGGAGT TCATGCACCA GTTGCCAGCA
    TTTGCCAACA TGACAATGTC TGTAAGACGA GAGCTCTGTG CAGTCATGGT GTTCGCTGTG
    GTCGAGCGGG CAGGAACTGT TGTACTAAAT GATGGGGAGG AGCTCGATTC CTGGTCAGTT
    ATTTTAAATG GCTCAGTAGA AGTGACATAT CCTGATGGCA GGACAGAAAT ACTTTGCATG
    GGGAACAGCT TTGGTGTGTC GCCAACCATG GAGAAGGAAT ACATGAAGGG AGTCATGAGA
    ACCAAAGTGG ATGACTGCCA GTTTGTGTGC ATCGCCCAGC AAGATTACTG CCGCATCCTC
    AATCAGGTGG AAAAGAACAT GCAGAAAGTG GAAGAGGAGG GTGAGATTGT GATGGTGAAA
    GAGCACAGAG AGCTTGATCG CACCGGAACC AGGAAAGGTC ATATTGTCAT CAAGGGAACA
    TCGGAACGCC TAACCATGCA TCTTGTGGAA GAACATTCTG TGGTTGACCC AACATATATT
    GAAGATTTTC TCCTCACTTA CAGAACTTTC CTTTCTAGTC CGATGGAAGT GGGAAAGAAA
    CTACTGGAGT GGTTTAATGA CCCTAGTCTC AGGGACAAGG TTACACGAGT AGTGTTGCTC
    TGGGTAAATA ATCACTTTAA TGACTTTGAA GGGGACCCAG CCATGACTCG ATTTTTGGAA
    GAATTTGAGA ACAATTTGGA ACGTGAGAAA ATGGGTGGCC ACCTAAGGCT ATTAAATATT
    GCATGTGCTG CCAAAGCAAA AAGAAGATTG ATAACTCTAA CAAAGCCCTC CCGAGAAGCC
    CCCTTGCCTT TTAACCTGCT GGGAGGGTCA GACAAGGGAT TTGGCATCTT TGTAGACAGC
    GTGGATCCAG GAAGCAAAGC AGCAGAGACT GGCTTAAAGC GAGGGGATCA GATTTTGGAG
    GTAAATGGGC AGAACTTTGA AAACATTCAA CTTACCAAGG CTTTGGAAAT CCTAAGGAAC
    AATACTCACT TGTCTCTCAC TGTGAAAACA AATTTATTTG TGTTTAAGGA ACTTCTGGCC
    AGAATGTTAG AGGAAAAGAG AAATAGTGCC CCACACTTGC CCAAAATTGG AGACATCAAA
    AAAGCCAGCC GCTATTCTAT TCCAGACCTT GCCGTTGATG TTGAACAAGT TATTGGACTT
    GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT ACTGTCGGAG GACGAAACAA GCTGAAAAAG
    ATTCTTGACA AGACACGGAT CAGTATCCTG CCTCAAAAGC CTTACAATGA TATTGGAATC
    GGTCAGTCTC AAGATGACAG CATTGTCGGT CTCCGACAGA CCAAGCACAT GCCGGCTACA
    CTACCTGTCA GTGGGACATT ATCTTCCAGC AATCCGGATT TACTGCAATC CCATCACCGC
    ATCTTAGACT TTAATACTAC ACCAGAACAA CCGGACCAGG TGCTGAGAGT TTTCAAGGCC
    GATCAGCAGA GCCGTTACAT AATGATAAGC AAGGACACCA CCGCTAAGGA AGTGGTCATC
    CAGGCAATCA GAGAATTTGC ATTAACTGCG GTACCTGATG CCTACTCATT GTGCGAAGTG
    TCTGTCACCC CTGAAGGTGT CATCAAACAA AGGCGGCTTC CTGATCAGCT TTCCAAGCTG
    GCTGACAGAA TACAACTCAG TGGCAGGTAT TACCTAAAAA ACAACATGGA AACTGAAACA
    CTATGCTCAG ATGAAGATGC CCAAGATCTT CTAAGGGAAA GTCAGATCTC TTTGCTACAG
    CTCAGCACCA TTGAAGTGGC AACACAGCTG TCTATGAGAA ATTTTGAGCT ATTTAGGAAC
    ATTGAGCCCA CCGAGTACAT TGATGACTTG TTCAAACTGA AAATGAAAAC TGGCTGCACC
    AACCTCAAAA ACTTCGAAGA AGTCATTAAC CAAGAGACCT TCTGGGTGGC CTCAGAAATT
    CTAAGGGAAA CTAACCAGCT AAAACGGATG AAAATCATCA AGCATTTTAT CAAGATCGCA
    CTACATTGTA GAGAGTGCAA GAACTTCAAC TCCATGTTTG CTATCATTAG TGGCTTAAAT
    TTGGCACCAG TTTCAAGACT CCGAACAACT TGGGAAAAAC TCCCAAGCAA ATATGAGAAA
    CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC CCTTCCAGGA ACATGGCTAA ATATCGGAAT
    GTGCTGAATA GTCAGAACCT TCAGCCTCCC ATTATACCTT TATTTCCTGT CATTAAGAAA
    GACCTCACCT TCCTTCATGA GGGCAATGAC TCCAAAGTAG ACGGATTAGT TAACTTTGAG
    AAGCTTAGGA TGATTGCAAA AGAAATCCGC CATGTGGGAA GAATGGCTTC AGTAAATATG
    GATCCAGCTC TTATGTTCCG AACCAGGAAG AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT
    CAAGGCAGCA CCAATGCTAC GGTCCTCGAC GTCGCCCAGT CGGGGGGACA CAAAAAACGT
    GTGCGTCGCA GCTCTTTCCT TAATGCCAAG AAACTGTACG AAGATGCTCA GATGGCTCGC
    AAGGTGAAGC AATACTTATC TGATCTAGAT CTTGAAATGG ATGAAGAGAA TCTTCAGACA
    CTTTCTGTAC AGTGTGAACC AGCAACCAAC ACATTGCCAA AAAATCCATG TGAGAAGAGA
    GCAGGGAAGC CAGACACATC GCCTGTGGCA CCACGGGCAG CAGCACAACA CAAACAGCAA
    CAGCAGCAGC AAACTAAGGT CAACCAGGCA TTGCAAGTTC CTGCTGTGGC TCTTTACCCA
    TCTCGTAAGA AAGTGCCAGT CAAGGATCTT CCACCCTTCG GCATCAACTC TCCCCAAGCT
    TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA GGAAGTTTGG AAAGGCACAA GAAGCAAGCA
    GAGGACACCA TATCAAATGC ATCCTCGCAG CTTTCATCCC CTCCGACATC CCCGCAGTGC
    TCTCCAAGGA AAGGTTACAC AATGGCAGCT AGTGGCACAG TGGATAACTT CTCTGATTCA
    GGGCACAGTG AAATCTCCTC CCGCTCCAGC ATTGTCAGTA ACTCTTCTTT TGACTGCGTG
    CACTTGCATG ATGACCGGCG GCAGAGGCAT TCCCTAAGCA TAGTGGAGAC CAATCTTGGT
    GTGGGAAGGG CTGACAGAAG AGCCATTATA GATGCAGACC AGTGCAGCCT AGGCTCTTAC
    GCGCCAGTGG CAGAGACTCG GGGCCTCTAT GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC
    ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT CGAGCTTCTT TAGATGCAGC AGATAGTGGC
    CGTGGAAGCT GGACTTCATG TTCCAGCGGA TCCCATGATA ACATACAAAC TATCCCACAT
    CAGAGAAGCT GGGAGACTCT TCCTTTTGGT CATGTTCATT TTGATAACAC AGCTGATGGG
    TCAGGGTTTT GGGCCACAGG TGGCCACATG GACCACGGGA TATTTCCTGA TCATAGCACA
    AAATATGGCA GATACAGCCA AGGAAGAGAA CCTTTGGATC AAGCACAGTC AAGAGCCAGC
    TGGGCATCAT CAACAGGCTA CTGGGGCGAG GACTCAGAAG GTGATACCGG CACCATTAAG
    AGAAGAGGTG GGAAAGATGT GGCCCTTGAA GCTGAAACCA GCAGTATAAC GTCAATGACT
    CCTGAAGAAA CAAAGCCTCT CCCTGTACCC ACACATGTAG CTGTGACTGC AAGTTCTGCA
    AAAGGGCTCA TTGTGTATTG TGTTACCACA GCAAGGAAAG AAGGCCGGTA CAGAGAACCG
    CCGCCCACAC CTCCAGGCTA TGTAGGAATC CCCATAGCTG ACTTTGCCGA TGGACACCCA
    CCTCTATCCA GAAAGCCACC AGATTACAAT GTGGCCTTGC AGCGTTCCAG AATGATGGCT
    CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA ACCTCTCCGC CATCACATAA TCAGACTTCT
    GGCAGGACTG CAAACAAACC CCAGTGGCAT AAACCAAACG AGGCTGACCC AAGGCTTGCT
    CTCTATCAGG CACAAGGGGT TACAGAAAAA GATGATGGTA ATTTTTTTGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811968.1
    CDS587..5224
    Translation

    Target ORF information:

    RefSeq Version XM_012956514.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956514.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAGCGCCA GGCATCTCAC 
    CGGCAGTCTC GCCGGAGGTT CCGCAAGATC AATCAGAAAG GCGAGAGGCA AACCATTATT
    GATACAGTGG AATCCTATCC TGTTGGAAAA CCACCTTTGC CAAGAGGGTA TCATACGGAA
    GGCTCTAAGC CTCAGCTCCC TGCTGATTTC ACTAAACTGC ACATCACAGA CAGTCTACAT
    CCACAGGTGA CGCACGTGTC CTCTAGTCAT TCAGGATGTA GCATCACTAG CGATTCTGGG
    AGCAGCAGCT TGTCTGACAT CTACCAGGCC ACAGAAAGTG AGCCAGGAGA TATGGACCTG
    AGTGGGCTGC CAGAGACCGC TGTAGACTCA GAAGATGACG ATGATGAAGA GGATATTGAA
    AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC ATTGTCAGAG ACTGTCTGGA GAAGGATCCA
    ATTGACAGGA CTGACGACGA TATTGAACAA CTTCTGGAGT TCATGCACCA GTTGCCAGCA
    TTTGCCAACA TGACAATGTC TGTAAGACGA GAGCTCTGTG CAGTCATGGT GTTCGCTGTG
    GTCGAGCGGG CAGGAACTGT TGTACTAAAT GATGGGGAGG AGCTCGATTC CTGGTCAGTT
    ATTTTAAATG GCTCAGTAGA AGTGACATAT CCTGATGGCA GGACAGAAAT ACTTTGCATG
    GGGAACAGCT TTGGTGTGTC GCCAACCATG GAGAAGGAAT ACATGAAGGG AGTCATGAGA
    ACCAAAGTGG ATGACTGCCA GTTTGTGTGC ATCGCCCAGC AAGATTACTG CCGCATCCTC
    AATCAGGTGG AAAAGAACAT GCAGAAAGTG GAAGAGGAGG GTGAGATTGT GATGGTGAAA
    GAGCACAGAG AGCTTGATCG CACCGGAACC AGGAAAGGTC ATATTGTCAT CAAGGGAACA
    TCGGAACGCC TAACCATGCA TCTTGTGGAA GAACATTCTG TGGTTGACCC AACATATATT
    GAAGATTTTC TCCTCACTTA CAGAACTTTC CTTTCTAGTC CGATGGAAGT GGGAAAGAAA
    CTACTGGAGT GGTTTAATGA CCCTAGTCTC AGGGACAAGG TTACACGAGT AGTGTTGCTC
    TGGGTAAATA ATCACTTTAA TGACTTTGAA GGGGACCCAG CCATGACTCG ATTTTTGGAA
    GAATTTGAGA ACAATTTGGA ACGTGAGAAA ATGGGTGGCC ACCTAAGGCT ATTAAATATT
    GCATGTGCTG CCAAAGCAAA AAGAAGATTG ATAACTCTAA CAAAGCCCTC CCGAGAAGCC
    CCCTTGCCTT TTAACCTGCT GGGAGGGTCA GACAAGGGAT TTGGCATCTT TGTAGACAGC
    GTGGATCCAG GAAGCAAAGC AGCAGAGACT GGCTTAAAGC GAGGGGATCA GATTTTGGAG
    GTAAATGGGC AGAACTTTGA AAACATTCAA CTTACCAAGG CTTTGGAAAT CCTAAGGAAC
    AATACTCACT TGTCTCTCAC TGTGAAAACA AATTTATTTG TGTTTAAGGA ACTTCTGGCC
    AGAATGTTAG AGGAAAAGAG AAATAGTGCC CCACACTTGC CCAAAATTGG AGACATCAAA
    AAAGCCAGCC GCTATTCTAT TCCAGACCTT GCCGTTGATG TTGAACAAGT TATTGGACTT
    GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT ACTGTCGGAG GACGAAACAA GCTGAAAAAG
    ATTCTTGACA AGACACGGAT CAGTATCCTG CCTCAAAAGC CTTACAATGA TATTGGAATC
    GGTCAGTCTC AAGATGACAG CATTGTCGGT CTCCGACAGA CCAAGCACAT GCCGGCTACA
    CTACCTGTCA GTGGGACATT ATCTTCCAGC AATCCGGATT TACTGCAATC CCATCACCGC
    ATCTTAGACT TTAATACTAC ACCAGAACAA CCGGACCAGG TGCTGAGAGT TTTCAAGGCC
    GATCAGCAGA GCCGTTACAT AATGATAAGC AAGGACACCA CCGCTAAGGA AGTGGTCATC
    CAGGCAATCA GAGAATTTGC ATTAACTGCG GTACCTGATG CCTACTCATT GTGCGAAGTG
    TCTGTCACCC CTGAAGGTGT CATCAAACAA AGGCGGCTTC CTGATCAGCT TTCCAAGCTG
    GCTGACAGAA TACAACTCAG TGGCAGGTAT TACCTAAAAA ACAACATGGA AACTGAAACA
    CTATGCTCAG ATGAAGATGC CCAAGATCTT CTAAGGGAAA GTCAGATCTC TTTGCTACAG
    CTCAGCACCA TTGAAGTGGC AACACAGCTG TCTATGAGAA ATTTTGAGCT ATTTAGGAAC
    ATTGAGCCCA CCGAGTACAT TGATGACTTG TTCAAACTGA AAATGAAAAC TGGCTGCACC
    AACCTCAAAA ACTTCGAAGA AGTCATTAAC CAAGAGACCT TCTGGGTGGC CTCAGAAATT
    CTAAGGGAAA CTAACCAGCT AAAACGGATG AAAATCATCA AGCATTTTAT CAAGATCGCA
    CTACATTGTA GAGAGTGCAA GAACTTCAAC TCCATGTTTG CTATCATTAG TGGCTTAAAT
    TTGGCACCAG TTTCAAGACT CCGAACAACT TGGGAAAAAC TCCCAAGCAA ATATGAGAAA
    CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC CCTTCCAGGA ACATGGCTAA ATATCGGAAT
    GTGCTGAATA GTCAGAACCT TCAGCCTCCC ATTATACCTT TATTTCCTGT CATTAAGAAA
    GACCTCACCT TCCTTCATGA GGGCAATGAC TCCAAAGTAG ACGGATTAGT TAACTTTGAG
    AAGCTTAGGA TGATTGCAAA AGAAATCCGC CATGTGGGAA GAATGGCTTC AGTAAATATG
    GATCCAGCTC TTATGTTCCG AACCAGGAAG AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT
    CAAGGCAGCA CCAATGCTAC GGTCCTCGAC GTCGCCCAGT CGGGGGGACA CAAAAAACGT
    GTGCGTCGCA GCTCTTTCCT TAATGCCAAG AAACTGTACG AAGATGCTCA GATGGCTCGC
    AAGGTGAAGC AATACTTATC TGATCTAGAT CTTGAAATGG ATGAAGAGAA TCTTCAGACA
    CTTTCTGTAC AGTGTGAACC AGCAACCAAC ACATTGCCAA AAAATCCATG TGAGAAGAGA
    GCAGGGAAGC CAGACACATC GCCTGTGGCA CCACGGGCAG CAGCACAACA CAAACAGCAA
    CAGCAGCAGC AAACTAAGGT CAACCAGGCA TTGCAAGTTC CTGCTGTGGC TCTTTACCCA
    TCTCGTAAGA AAGTGCCAGT CAAGGATCTT CCACCCTTCG GCATCAACTC TCCCCAAGCT
    TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA GGAAGTTTGG AAAGGCACAA GAAGCAAGCA
    GAGGACACCA TATCAAATGC ATCCTCGCAG CTTTCATCCC CTCCGACATC CCCGCAGTGC
    TCTCCAAGGA AAGGTTACAC AATGGCAGCT AGTGGCACAG TGGATAACTT CTCTGATTCA
    GGGCACAGTG AAATCTCCTC CCGCTCCAGC ATTGTCAGTA ACTCTTCTTT TGACTGCGTG
    CACTTGCATG ATGACCGGCG GCAGAGGCAT TCCCTAAGCA TAGTGGAGAC CAATCTTGGT
    GTGGGAAGGG CTGACAGAAG AGCCATTATA GATGCAGACC AGTGCAGCCT AGGCTCTTAC
    GCGCCAGTGG CAGAGACTCG GGGCCTCTAT GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC
    ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT CGAGCTTCTT TAGATGCAGC AGATAGTGGC
    CGTGGAAGCT GGACTTCATG TTCCAGCGGA TCCCATGATA ACATACAAAC TATCCCACAT
    CAGAGAAGCT GGGAGACTCT TCCTTTTGGT CATGTTCATT TTGATAACAC AGCTGATGGG
    TCAGGGTTTT GGGCCACAGG TGGCCACATG GACCACGGGA TATTTCCTGA TCATAGCACA
    AAATATGGCA GATACAGCCA AGGAAGAGAA CCTTTGGATC AAGCACAGTC AAGAGCCAGC
    TGGGCATCAT CAACAGGCTA CTGGGGCGAG GACTCAGAAG GTGATACCGG CACCATTAAG
    AGAAGAGGTG GGAAAGATGT GGCCCTTGAA GCTGAAACCA GCAGTATAAC GTCAATGACT
    CCTGAAGAAA CAAAGCCTCT CCCTGTACCC ACACATGTAG CTGTGACTGC AAGTTCTGCA
    AAAGGGCTCA TTGTGTATTG TGTTACCACA GCAAGGAAAG AAGGCCGGTA CAGAGAACCG
    CCGCCCACAC CTCCAGGCTA TGTAGGAATC CCCATAGCTG ACTTTGCCGA TGGACACCCA
    CCTCTATCCA GAAAGCCACC AGATTACAAT GTGGCCTTGC AGCGTTCCAG AATGATGGCT
    CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA ACCTCTCCGC CATCACATAA TCAGACTTCT
    GGCAGGACTG CAAACAAACC CCAGTGGCAT AAACCAAACG AGGCTGACCC AAGGCTTGCT
    CTCTATCAGG CACAAGGGGT TACAGAAAAA GATGATGGTA ATTTTTTTGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47598
    Clone ID Related Accession (Same CDS sequence) XM_012956519.3
    Accession Version XM_012956519.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAACCTC TTGCATTCCC ATCTAGTCAT GGAGTTATGG GCCAGCAGGA GAAGCATTCT 
    CTCCCTGCTG ATTTCACTAA ACTGCACATC ACAGACAGTC TACATCCACA GGTGACGCAC
    GTGTCCTCTA GTCATTCAGG ATGTAGCATC ACTAGCGATT CTGGGAGCAG CAGCTTGTCT
    GACATCTACC AGGCCACAGA AAGTGAGCCA GGAGATATGG ACCTGAGTGG GCTGCCAGAG
    ACCGCTGTAG ACTCAGAAGA TGACGATGAT GAAGAGGATA TTGAAAGAGC TTCTGACCCT
    TTAATGAGCA GGGACATTGT CAGAGACTGT CTGGAGAAGG ATCCAATTGA CAGGACTGAC
    GACGATATTG AACAACTTCT GGAGTTCATG CACCAGTTGC CAGCATTTGC CAACATGACA
    ATGTCTGTAA GACGAGAGCT CTGTGCAGTC ATGGTGTTCG CTGTGGTCGA GCGGGCAGGA
    ACTGTTGTAC TAAATGATGG GGAGGAGCTC GATTCCTGGT CAGTTATTTT AAATGGCTCA
    GTAGAAGTGA CATATCCTGA TGGCAGGACA GAAATACTTT GCATGGGGAA CAGCTTTGGT
    GTGTCGCCAA CCATGGAGAA GGAATACATG AAGGGAGTCA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TGTGCATCGC CCAGCAAGAT TACTGCCGCA TCCTCAATCA GGTGGAAAAG
    AACATGCAGA AAGTGGAAGA GGAGGGTGAG ATTGTGATGG TGAAAGAGCA CAGAGAGCTT
    GATCGCACCG GAACCAGGAA AGGTCATATT GTCATCAAGG GAACATCGGA ACGCCTAACC
    ATGCATCTTG TGGAAGAACA TTCTGTGGTT GACCCAACAT ATATTGAAGA TTTTCTCCTC
    ACTTACAGAA CTTTCCTTTC TAGTCCGATG GAAGTGGGAA AGAAACTACT GGAGTGGTTT
    AATGACCCTA GTCTCAGGGA CAAGGTTACA CGAGTAGTGT TGCTCTGGGT AAATAATCAC
    TTTAATGACT TTGAAGGGGA CCCAGCCATG ACTCGATTTT TGGAAGAATT TGAGAACAAT
    TTGGAACGTG AGAAAATGGG TGGCCACCTA AGGCTATTAA ATATTGCATG TGCTGCCAAA
    GCAAAAAGAA GATTGATAAC TCTAACAAAG CCCTCCCGAG AAGCCCCCTT GCCTTTTAAC
    CTGCTGGGAG GGTCAGACAA GGGATTTGGC ATCTTTGTAG ACAGCGTGGA TCCAGGAAGC
    AAAGCAGCAG AGACTGGCTT AAAGCGAGGG GATCAGATTT TGGAGGTAAA TGGGCAGAAC
    TTTGAAAACA TTCAACTTAC CAAGGCTTTG GAAATCCTAA GGAACAATAC TCACTTGTCT
    CTCACTGTGA AAACAAATTT ATTTGTGTTT AAGGAACTTC TGGCCAGAAT GTTAGAGGAA
    AAGAGAAATA GTGCCCCACA CTTGCCCAAA ATTGGAGACA TCAAAAAAGC CAGCCGCTAT
    TCTATTCCAG ACCTTGCCGT TGATGTTGAA CAAGTTATTG GACTTGAAAA AGTGAGCAAA
    AAGGGCAAAG CCAATACTGT CGGAGGACGA AACAAGCTGA AAAAGATTCT TGACAAGACA
    CGGATCAGTA TCCTGCCTCA AAAGCCTTAC AATGATATTG GAATCGGTCA GTCTCAAGAT
    GACAGCATTG TCGGTCTCCG ACAGACCAAG CACATGCCGG CTACACTACC TGTCAGTGGG
    ACATTATCTT CCAGCAATCC GGATTTACTG CAATCCCATC ACCGCATCTT AGACTTTAAT
    ACTACACCAG AACAACCGGA CCAGGTGCTG AGAGTTTTCA AGGCCGATCA GCAGAGCCGT
    TACATAATGA TAAGCAAGGA CACCACCGCT AAGGAAGTGG TCATCCAGGC AATCAGAGAA
    TTTGCATTAA CTGCGGTACC TGATGCCTAC TCATTGTGCG AAGTGTCTGT CACCCCTGAA
    GGTGTCATCA AACAAAGGCG GCTTCCTGAT CAGCTTTCCA AGCTGGCTGA CAGAATACAA
    CTCAGTGGCA GGTATTACCT AAAAAACAAC ATGGAAACTG AAACACTATG CTCAGATGAA
    GATGCCCAAG ATCTTCTAAG GGAAAGTCAG ATCTCTTTGC TACAGCTCAG CACCATTGAA
    GTGGCAACAC AGCTGTCTAT GAGAAATTTT GAGCTATTTA GGAACATTGA GCCCACCGAG
    TACATTGATG ACTTGTTCAA ACTGAAAATG AAAACTGGCT GCACCAACCT CAAAAACTTC
    GAAGAAGTCA TTAACCAAGA GACCTTCTGG GTGGCCTCAG AAATTCTAAG GGAAACTAAC
    CAGCTAAAAC GGATGAAAAT CATCAAGCAT TTTATCAAGA TCGCACTACA TTGTAGAGAG
    TGCAAGAACT TCAACTCCAT GTTTGCTATC ATTAGTGGCT TAAATTTGGC ACCAGTTTCA
    AGACTCCGAA CAACTTGGGA AAAACTCCCA AGCAAATATG AGAAACTCTT TCAAGACCTC
    CAGGACCTCT TTGACCCTTC CAGGAACATG GCTAAATATC GGAATGTGCT GAATAGTCAG
    AACCTTCAGC CTCCCATTAT ACCTTTATTT CCTGTCATTA AGAAAGACCT CACCTTCCTT
    CATGAGGGCA ATGACTCCAA AGTAGACGGA TTAGTTAACT TTGAGAAGCT TAGGATGATT
    GCAAAAGAAA TCCGCCATGT GGGAAGAATG GCTTCAGTAA ATATGGATCC AGCTCTTATG
    TTCCGAACCA GGAAGAAGAA GTGGAGGAGT CTGGGGTCTC TGAGTCAAGG CAGCACCAAT
    GCTACGGTCC TCGACGTCGC CCAGTCGGGG GGACACAAAA AACGTGTGCG TCGCAGCTCT
    TTCCTTAATG CCAAGAAACT GTACGAAGAT GCTCAGATGG CTCGCAAGGT GAAGCAATAC
    TTATCTGATC TAGATCTTGA AATGGATGAA GAGAATCTTC AGACACTTTC TGTACAGTGT
    GAACCAGCAA CCAACACATT GCCAAAAAAT CCATGTGAGA AGAGAGCAGG GAAGCCAGAC
    ACATCGCCTG TGGCACCACG GGCAGCAGCA CAACACAAAC AGCAACAGCA GCAGCAAACT
    AAGGTCAACC AGGCATTGCA AGTTCCTGCT GTGGCTCTTT ACCCATCTCG TAAGAAAGTG
    CCAGTCAAGG ATCTTCCACC CTTCGGCATC AACTCTCCCC AAGCTTTAAA GAAAATTCTT
    TCGTTATCAG AAGAAGGAAG TTTGGAAAGG CACAAGAAGC AAGCAGAGGA CACCATATCA
    AATGCATCCT CGCAGCTTTC ATCCCCTCCG ACATCCCCGC AGTGCTCTCC AAGGAAAGGT
    TACACAATGG CAGCTAGTGG CACAGTGGAT AACTTCTCTG ATTCAGGGCA CAGTGAAATC
    TCCTCCCGCT CCAGCATTGT CAGTAACTCT TCTTTTGACT GCGTGCACTT GCATGATGAC
    CGGCGGCAGA GGCATTCCCT AAGCATAGTG GAGACCAATC TTGGTGTGGG AAGGGCTGAC
    AGAAGAGCCA TTATAGATGC AGACCAGTGC AGCCTAGGCT CTTACGCGCC AGTGGCAGAG
    ACTCGGGGCC TCTATGCAGG TGCAACAGTT CTCTCCTCCC CCAGCATGGA GGAGCTTTCC
    CAGGATCAGG GGGATCGAGC TTCTTTAGAT GCAGCAGATA GTGGCCGTGG AAGCTGGACT
    TCATGTTCCA GCGGATCCCA TGATAACATA CAAACTATCC CACATCAGAG AAGCTGGGAG
    ACTCTTCCTT TTGGTCATGT TCATTTTGAT AACACAGCTG ATGGGTCAGG GTTTTGGGCC
    ACAGGTGGCC ACATGGACCA CGGGATATTT CCTGATCATA GCACAAAATA TGGCAGATAC
    AGCCAAGGAA GAGAACCTTT GGATCAAGCA CAGTCAAGAG CCAGCTGGGC ATCATCAACA
    GGCTACTGGG GCGAGGACTC AGAAGGTGAT ACCGGCACCA TTAAGAGAAG AGGTGGGAAA
    GATGTGGCCC TTGAAGCTGA AACCAGCAGT ATAACGTCAA TGACTCCTGA AGAAACAAAG
    CCTCTCCCTG TACCCACACA TGTAGCTGTG ACTGCAAGTT CTGCAAAAGG GCTCATTGTG
    TATTGTGTTA CCACAGCAAG GAAAGAAGGC CGGTACAGAG AACCGCCGCC CACACCTCCA
    GGCTATGTAG GAATCCCCAT AGCTGACTTT GCCGATGGAC ACCCACCTCT ATCCAGAAAG
    CCACCAGATT ACAATGTGGC CTTGCAGCGT TCCAGAATGA TGGCTCGGAG AAGTGAAAGT
    GAAGGGTCAG CAGCAACCTC TCCGCCATCA CATAATCAGA CTTCTGGCAG GACTGCAAAC
    AAACCCCAGT GGCATAAACC AAACGAGGCT GACCCAAGGC TTGCTCTCTA TCAGGCACAA
    GGGGTTACAG AAAAAGATGA TGGTAATTTT TTTGCCGATG ATGAGCAAGT ATCTGCCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811973.1
    CDS318..4820
    Translation

    Target ORF information:

    RefSeq Version XM_012956519.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956519.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAACCTC TTGCATTCCC ATCTAGTCAT GGAGTTATGG GCCAGCAGGA GAAGCATTCT 
    CTCCCTGCTG ATTTCACTAA ACTGCACATC ACAGACAGTC TACATCCACA GGTGACGCAC
    GTGTCCTCTA GTCATTCAGG ATGTAGCATC ACTAGCGATT CTGGGAGCAG CAGCTTGTCT
    GACATCTACC AGGCCACAGA AAGTGAGCCA GGAGATATGG ACCTGAGTGG GCTGCCAGAG
    ACCGCTGTAG ACTCAGAAGA TGACGATGAT GAAGAGGATA TTGAAAGAGC TTCTGACCCT
    TTAATGAGCA GGGACATTGT CAGAGACTGT CTGGAGAAGG ATCCAATTGA CAGGACTGAC
    GACGATATTG AACAACTTCT GGAGTTCATG CACCAGTTGC CAGCATTTGC CAACATGACA
    ATGTCTGTAA GACGAGAGCT CTGTGCAGTC ATGGTGTTCG CTGTGGTCGA GCGGGCAGGA
    ACTGTTGTAC TAAATGATGG GGAGGAGCTC GATTCCTGGT CAGTTATTTT AAATGGCTCA
    GTAGAAGTGA CATATCCTGA TGGCAGGACA GAAATACTTT GCATGGGGAA CAGCTTTGGT
    GTGTCGCCAA CCATGGAGAA GGAATACATG AAGGGAGTCA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TGTGCATCGC CCAGCAAGAT TACTGCCGCA TCCTCAATCA GGTGGAAAAG
    AACATGCAGA AAGTGGAAGA GGAGGGTGAG ATTGTGATGG TGAAAGAGCA CAGAGAGCTT
    GATCGCACCG GAACCAGGAA AGGTCATATT GTCATCAAGG GAACATCGGA ACGCCTAACC
    ATGCATCTTG TGGAAGAACA TTCTGTGGTT GACCCAACAT ATATTGAAGA TTTTCTCCTC
    ACTTACAGAA CTTTCCTTTC TAGTCCGATG GAAGTGGGAA AGAAACTACT GGAGTGGTTT
    AATGACCCTA GTCTCAGGGA CAAGGTTACA CGAGTAGTGT TGCTCTGGGT AAATAATCAC
    TTTAATGACT TTGAAGGGGA CCCAGCCATG ACTCGATTTT TGGAAGAATT TGAGAACAAT
    TTGGAACGTG AGAAAATGGG TGGCCACCTA AGGCTATTAA ATATTGCATG TGCTGCCAAA
    GCAAAAAGAA GATTGATAAC TCTAACAAAG CCCTCCCGAG AAGCCCCCTT GCCTTTTAAC
    CTGCTGGGAG GGTCAGACAA GGGATTTGGC ATCTTTGTAG ACAGCGTGGA TCCAGGAAGC
    AAAGCAGCAG AGACTGGCTT AAAGCGAGGG GATCAGATTT TGGAGGTAAA TGGGCAGAAC
    TTTGAAAACA TTCAACTTAC CAAGGCTTTG GAAATCCTAA GGAACAATAC TCACTTGTCT
    CTCACTGTGA AAACAAATTT ATTTGTGTTT AAGGAACTTC TGGCCAGAAT GTTAGAGGAA
    AAGAGAAATA GTGCCCCACA CTTGCCCAAA ATTGGAGACA TCAAAAAAGC CAGCCGCTAT
    TCTATTCCAG ACCTTGCCGT TGATGTTGAA CAAGTTATTG GACTTGAAAA AGTGAGCAAA
    AAGGGCAAAG CCAATACTGT CGGAGGACGA AACAAGCTGA AAAAGATTCT TGACAAGACA
    CGGATCAGTA TCCTGCCTCA AAAGCCTTAC AATGATATTG GAATCGGTCA GTCTCAAGAT
    GACAGCATTG TCGGTCTCCG ACAGACCAAG CACATGCCGG CTACACTACC TGTCAGTGGG
    ACATTATCTT CCAGCAATCC GGATTTACTG CAATCCCATC ACCGCATCTT AGACTTTAAT
    ACTACACCAG AACAACCGGA CCAGGTGCTG AGAGTTTTCA AGGCCGATCA GCAGAGCCGT
    TACATAATGA TAAGCAAGGA CACCACCGCT AAGGAAGTGG TCATCCAGGC AATCAGAGAA
    TTTGCATTAA CTGCGGTACC TGATGCCTAC TCATTGTGCG AAGTGTCTGT CACCCCTGAA
    GGTGTCATCA AACAAAGGCG GCTTCCTGAT CAGCTTTCCA AGCTGGCTGA CAGAATACAA
    CTCAGTGGCA GGTATTACCT AAAAAACAAC ATGGAAACTG AAACACTATG CTCAGATGAA
    GATGCCCAAG ATCTTCTAAG GGAAAGTCAG ATCTCTTTGC TACAGCTCAG CACCATTGAA
    GTGGCAACAC AGCTGTCTAT GAGAAATTTT GAGCTATTTA GGAACATTGA GCCCACCGAG
    TACATTGATG ACTTGTTCAA ACTGAAAATG AAAACTGGCT GCACCAACCT CAAAAACTTC
    GAAGAAGTCA TTAACCAAGA GACCTTCTGG GTGGCCTCAG AAATTCTAAG GGAAACTAAC
    CAGCTAAAAC GGATGAAAAT CATCAAGCAT TTTATCAAGA TCGCACTACA TTGTAGAGAG
    TGCAAGAACT TCAACTCCAT GTTTGCTATC ATTAGTGGCT TAAATTTGGC ACCAGTTTCA
    AGACTCCGAA CAACTTGGGA AAAACTCCCA AGCAAATATG AGAAACTCTT TCAAGACCTC
    CAGGACCTCT TTGACCCTTC CAGGAACATG GCTAAATATC GGAATGTGCT GAATAGTCAG
    AACCTTCAGC CTCCCATTAT ACCTTTATTT CCTGTCATTA AGAAAGACCT CACCTTCCTT
    CATGAGGGCA ATGACTCCAA AGTAGACGGA TTAGTTAACT TTGAGAAGCT TAGGATGATT
    GCAAAAGAAA TCCGCCATGT GGGAAGAATG GCTTCAGTAA ATATGGATCC AGCTCTTATG
    TTCCGAACCA GGAAGAAGAA GTGGAGGAGT CTGGGGTCTC TGAGTCAAGG CAGCACCAAT
    GCTACGGTCC TCGACGTCGC CCAGTCGGGG GGACACAAAA AACGTGTGCG TCGCAGCTCT
    TTCCTTAATG CCAAGAAACT GTACGAAGAT GCTCAGATGG CTCGCAAGGT GAAGCAATAC
    TTATCTGATC TAGATCTTGA AATGGATGAA GAGAATCTTC AGACACTTTC TGTACAGTGT
    GAACCAGCAA CCAACACATT GCCAAAAAAT CCATGTGAGA AGAGAGCAGG GAAGCCAGAC
    ACATCGCCTG TGGCACCACG GGCAGCAGCA CAACACAAAC AGCAACAGCA GCAGCAAACT
    AAGGTCAACC AGGCATTGCA AGTTCCTGCT GTGGCTCTTT ACCCATCTCG TAAGAAAGTG
    CCAGTCAAGG ATCTTCCACC CTTCGGCATC AACTCTCCCC AAGCTTTAAA GAAAATTCTT
    TCGTTATCAG AAGAAGGAAG TTTGGAAAGG CACAAGAAGC AAGCAGAGGA CACCATATCA
    AATGCATCCT CGCAGCTTTC ATCCCCTCCG ACATCCCCGC AGTGCTCTCC AAGGAAAGGT
    TACACAATGG CAGCTAGTGG CACAGTGGAT AACTTCTCTG ATTCAGGGCA CAGTGAAATC
    TCCTCCCGCT CCAGCATTGT CAGTAACTCT TCTTTTGACT GCGTGCACTT GCATGATGAC
    CGGCGGCAGA GGCATTCCCT AAGCATAGTG GAGACCAATC TTGGTGTGGG AAGGGCTGAC
    AGAAGAGCCA TTATAGATGC AGACCAGTGC AGCCTAGGCT CTTACGCGCC AGTGGCAGAG
    ACTCGGGGCC TCTATGCAGG TGCAACAGTT CTCTCCTCCC CCAGCATGGA GGAGCTTTCC
    CAGGATCAGG GGGATCGAGC TTCTTTAGAT GCAGCAGATA GTGGCCGTGG AAGCTGGACT
    TCATGTTCCA GCGGATCCCA TGATAACATA CAAACTATCC CACATCAGAG AAGCTGGGAG
    ACTCTTCCTT TTGGTCATGT TCATTTTGAT AACACAGCTG ATGGGTCAGG GTTTTGGGCC
    ACAGGTGGCC ACATGGACCA CGGGATATTT CCTGATCATA GCACAAAATA TGGCAGATAC
    AGCCAAGGAA GAGAACCTTT GGATCAAGCA CAGTCAAGAG CCAGCTGGGC ATCATCAACA
    GGCTACTGGG GCGAGGACTC AGAAGGTGAT ACCGGCACCA TTAAGAGAAG AGGTGGGAAA
    GATGTGGCCC TTGAAGCTGA AACCAGCAGT ATAACGTCAA TGACTCCTGA AGAAACAAAG
    CCTCTCCCTG TACCCACACA TGTAGCTGTG ACTGCAAGTT CTGCAAAAGG GCTCATTGTG
    TATTGTGTTA CCACAGCAAG GAAAGAAGGC CGGTACAGAG AACCGCCGCC CACACCTCCA
    GGCTATGTAG GAATCCCCAT AGCTGACTTT GCCGATGGAC ACCCACCTCT ATCCAGAAAG
    CCACCAGATT ACAATGTGGC CTTGCAGCGT TCCAGAATGA TGGCTCGGAG AAGTGAAAGT
    GAAGGGTCAG CAGCAACCTC TCCGCCATCA CATAATCAGA CTTCTGGCAG GACTGCAAAC
    AAACCCCAGT GGCATAAACC AAACGAGGCT GACCCAAGGC TTGCTCTCTA TCAGGCACAA
    GGGGTTACAG AAAAAGATGA TGGTAATTTT TTTGCCGATG ATGAGCAAGT ATCTGCCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23255
    Clone ID Related Accession (Same CDS sequence) NM_001127050.1
    Accession Version NM_001127050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124563.1. ##Evidence-Data-START## Transcript exon combination :: BC124563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTTTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAGTC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGTGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTAGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAGATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCACG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC ATCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGCAAGGAA AGAAGGCCGG
    TACAGAGAAC CGCCGCCCAC ACCTCCAGGC TATGTAGGAA TCCCCATAGC TGACTTTGCC
    GATGGACACC CACCTCTATC CAGAAAGCCA CCAGATTACA ATGTGGCCTT GCAGCGTTCC
    AGAATGATGG CTCGGAGAAG TGAAAGTGAA GGGTCAGCAG CAACCTCTCC GCCATCACAT
    AATCAGACTT CTGGCAGGAC TGCAAACAAA CCCCAGTGGC ATAAACCAAA CGAGGCTGAC
    CCAAGGCTTG CTCTCTATCA GGCACAAGGG GTTACAGAAA AAGATGATGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120522.1
    CDS367..5304
    Translation

    Target ORF information:

    RefSeq Version NM_001127050.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTTTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAGTC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGTGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTAGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAGATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCACG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC ATCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGCAAGGAA AGAAGGCCGG
    TACAGAGAAC CGCCGCCCAC ACCTCCAGGC TATGTAGGAA TCCCCATAGC TGACTTTGCC
    GATGGACACC CACCTCTATC CAGAAAGCCA CCAGATTACA ATGTGGCCTT GCAGCGTTCC
    AGAATGATGG CTCGGAGAAG TGAAAGTGAA GGGTCAGCAG CAACCTCTCC GCCATCACAT
    AATCAGACTT CTGGCAGGAC TGCAAACAAA CCCCAGTGGC ATAAACCAAA CGAGGCTGAC
    CCAAGGCTTG CTCTCTATCA GGCACAAGGG GTTACAGAAA AAGATGATGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31949
    Clone ID Related Accession (Same CDS sequence) XM_012956489.2
    Accession Version XM_012956489.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGGTAAT TTTTTTGCCG ATGATGAGCA AGTATCTGCC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811943.1
    CDS470..5455
    Translation

    Target ORF information:

    RefSeq Version XM_012956489.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956489.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGGTAAT TTTTTTGCCG ATGATGAGCA AGTATCTGCC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31950
    Clone ID Related Accession (Same CDS sequence) XM_012956494.1
    Accession Version XM_012956494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGCCGAT GATGAGCAAG TATCTGCCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811948.1
    CDS354..5327
    Translation

    Target ORF information:

    RefSeq Version XM_012956494.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GTGTATTGTG TTACCACAGC AAGGAAAGAA GGCCGGTACA GAGAACCGCC GCCCACACCT
    CCAGGCTATG TAGGAATCCC CATAGCTGAC TTTGCCGATG GACACCCACC TCTATCCAGA
    AAGCCACCAG ATTACAATGT GGCCTTGCAG CGTTCCAGAA TGATGGCTCG GAGAAGTGAA
    AGTGAAGGGT CAGCAGCAAC CTCTCCGCCA TCACATAATC AGACTTCTGG CAGGACTGCA
    AACAAACCCC AGTGGCATAA ACCAAACGAG GCTGACCCAA GGCTTGCTCT CTATCAGGCA
    CAAGGGGTTA CAGAAAAAGA TGATGCCGAT GATGAGCAAG TATCTGCCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31951
    Clone ID Related Accession (Same CDS sequence) XM_012956496.2
    Accession Version XM_012956496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTTTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAGTC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGTGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTAGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAGATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCACG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC ATCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGTGTATTG TGTTACCACA
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811950.1
    CDS469..5436
    Translation

    Target ORF information:

    RefSeq Version XM_012956496.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGCTCCC TGCTGATTTC
    ACTAAACTGC ACATCACAGA CAGTCTACAT CCACAGGTGA CGCACGTGTC CTCTAGTCAT
    TCAGGATGTA GCATCACTAG CGATTCTGGG AGCAGCAGCT TGTCTGACAT CTACCAGGCC
    ACAGAAAGTG AGCCAGGAGA TATGGACCTG AGTGGGCTGC CAGAGACCGC TGTAGACTCA
    GAAGATGACG ATGATGAAGA GGATATTGAA AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC
    ATTGTCAGAG ACTGTCTGGA GAAGGATCCA ATTGACAGGA CTGACGACGA TATTGAACAA
    CTTCTGGAGT TCATGCACCA GTTGCCAGCA TTTGCCAACA TGACAATGTC TGTAAGACGA
    GAGCTCTGTG CAGTCATGGT GTTCGCTGTG GTCGAGCGGG CAGGAACTGT TGTACTAAAT
    GATGGGGAGG AGCTCGATTC CTGGTCAGTT ATTTTAAATG GCTCAGTAGA AGTGACATAT
    CCTGATGGCA GGACAGAAAT ACTTTGCATG GGGAACAGCT TTGGTGTGTC GCCAACCATG
    GAGAAGGAAT ACATGAAGGG AGTCATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTGTGC
    ATCGCCCAGC AAGATTACTG CCGCATCCTC AATCAGGTGG AAAAGAACAT GCAGAAAGTG
    GAAGAGGAGG GTGAGATTGT GATGGTGAAA GAGCACAGAG AGCTTGATCG CACCGGAACC
    AGGAAAGGTC ATATTGTCAT CAAGGGAACA TCGGAACGCC TAACCATGCA TCTTGTGGAA
    GAACATTCTG TGGTTGACCC AACATATATT GAAGATTTTC TCCTCACTTA CAGAACTTTC
    CTTTCTAGTC CGATGGAAGT GGGAAAGAAA CTACTGGAGT GGTTTAATGA CCCTAGTCTC
    AGGGACAAGG TTACACGAGT AGTTTTGCTC TGGGTAAATA ATCACTTTAA TGACTTTGAA
    GGGGACCCAG CCATGACTCG ATTTTTGGAA GAATTTGAGA ACAATTTGGA ACGTGAGAAA
    ATGGGTGGCC ACCTAAGGCT ATTAAATATT GCATGTGCTG CCAAAGCAAA AAGAAGATTG
    ATAACTCTAA CAAAGCCCTC CCGAGAAGCC CCCTTGCCTT TTAACCTGCT GGGAGGGTCA
    GACAAGGGAT TTGGCATCTT TGTAGACAGC GTGGATCCAG GAAGCAAAGC AGCAGAGACT
    GGCTTAAAGC GAGGGGATCA GATTTTGGAG GTAAATGGGC AGAACTTTGA AAACATTCAA
    CTTACCAAGG CTTTGGAAAT CCTAAGGAAC AATACTCACT TGTCTCTCAC TGTGAAAACA
    AATTTATTTG TGTTTAAGGA ACTTCTGGCC AGAATGTTAG AGGAAAAGAG AAATAGTGCC
    CCACACTTGC CCAAAATTGG AGACATCAAA AAAGCCAGCC GCTATTCTAT TCCAGACCTT
    GCCGTTGATG TTGAACAAGT TATTGGACTT GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT
    ACTGTCGGAG GACGAAACAA GCTGAAAAAG ATTCTTGACA AGACACGGAT CAGTATCCTG
    CCTCAAAAGC CTTACAATGA TATTGGAATC GGTCAGTCTC AAGATGACAG CATTGTCGGT
    CTCCGACAGA CCAAGCACAT GCCGGCTACA CTACCTGTCA GTGGGACATT ATCTTCCAGC
    AATCCGGATT TACTGCAGTC CCATCACCGC ATCTTAGACT TTAATACTAC ACCAGAACAA
    CCGGACCAGG TGCTGAGAGT TTTCAAGGCC GATCAGCAGA GCCGTTACAT AATGATAAGC
    AAGGACACCA CCGCTAAGGA AGTGGTCATC CAGGCAATCA GAGAATTTGC ATTAACTGCG
    GTACCTGATG CCTACTCATT GTGTGAAGTG TCTGTCACCC CTGAAGGTGT CATCAAACAA
    AGGCGGCTTC CTGATCAGCT TTCCAAGCTG GCTGACAGAA TACAACTCAG TGGCAGGTAT
    TACCTAAAAA ACAACATGGA AACTGAAACA CTATGCTCAG ATGAAGATGC CCAAGATCTT
    CTAAGGGAAA GTCAGATCTC TTTGCTACAG CTCAGCACCA TTGAAGTGGC AACACAGCTG
    TCTATGAGAA ATTTTGAGCT ATTTAGGAAC ATTGAGCCCA CCGAGTACAT TGATGACTTG
    TTCAAACTGA AAATGAAAAC TGGCTGCACC AACCTCAAAA ACTTCGAAGA AGTCATTAAC
    CAAGAGACCT TCTGGGTAGC CTCAGAAATT CTAAGGGAAA CTAACCAGCT AAAACGGATG
    AAGATCATCA AGCATTTTAT CAAGATCGCA CTACATTGTA GAGAGTGCAA GAACTTCAAC
    TCCATGTTTG CTATCATTAG TGGCTTAAAT TTGGCACCAG TTTCAAGACT CCGAACAACT
    TGGGAAAAAC TCCCAAGCAA ATATGAGAAA CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC
    CCTTCCAGGA ACATGGCTAA ATATCGGAAT GTGCTGAATA GTCAGAACCT TCAGCCTCCC
    ATTATACCTT TATTTCCTGT CATTAAGAAA GACCTCACCT TCCTTCATGA GGGCAATGAC
    TCCAAAGTAG ACGGATTAGT TAACTTTGAG AAGCTTAGGA TGATTGCAAA AGAAATCCGC
    CATGTGGGAA GAATGGCTTC AGTAAATATG GATCCAGCTC TTATGTTCCG AACCAGGAAG
    AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT CAAGGCAGCA CCAATGCTAC GGTCCTCGAC
    GTCGCCCAGT CGGGGGGACA CAAAAAACGT GTGCGTCGCA GCTCTTTCCT TAATGCCAAG
    AAACTGTACG AAGATGCTCA GATGGCTCGC AAGGTGAAGC AATACTTATC TGATCTAGAT
    CTTGAAATGG ATGAAGAGAA TCTTCAGACA CTTTCTGTAC AGTGTGAACC AGCAACCAAC
    ACATTGCCAA AAAATCCATG TGAGAAGAGA GCAGGGAAGC CAGACACATC GCCTGTGGCA
    CCACGGGCAG CAGCACAACA CAAACAGCAA CAGCAGCAGC AAACTAAGGT CAACCAGGCA
    TTGCAAGTTC CTGCTGTGGC TCTTTACCCA TCTCGTAAGA AAGTGCCAGT CAAGGATCTT
    CCACCCTTCG GCATCAACTC TCCCCAAGCT TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA
    GGAAGTTTGG AAAGGCACAA GAAGCAAGCA GAGGACACCA TATCAAATGC ATCCTCGCAG
    CTTTCATCCC CTCCGACATC CCCGCAGTGC TCTCCAAGGA AAGGTTACAC AATGGCAGCT
    AGTGGCACAG TGGATAACTT CTCTGATTCA GGGCACAGTG AAATCTCCTC CCGCTCCAGC
    ATTGTCAGTA ACTCTTCTTT TGACTGCGTG CACTTGCACG ATGACCGGCG GCAGAGGCAT
    TCCCTAAGCA TAGTGGAGAC CAATCTTGGT GTGGGAAGGG CTGACAGAAG AGCCATTATA
    GATGCAGACC AGTGCAGCCT AGGCTCTTAC GCGCCAGTGG CAGAGACTCG GGGCCTCTAT
    GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT
    CGAGCTTCTT TAGATGCAGC AGATAGTGGC CGTGGAAGCT GGACTTCATG TTCCAGCGGA
    TCCCATGATA ACATACAAAC TATCCCACAT CAGAGAAGCT GGGAGACTCT TCCTTTTGGT
    CATGTTCATT TTGATAACAC AGCTGATGGG TCAGGGTTTT GGGCCACAGG TGGCCACATG
    GACCACGGGA TATTTCCTGA TCATAGCACA AAATATGGCA GATACAGCCA AGGAAGAGAA
    CCTTTGGATC AAGCACAGTC AAGAGCCAGC TGGGCATCAT CAACAGGCTA CTGGGGCGAG
    GACTCAGAAG GTGATACCGG CACCATTAAG AGAAGAGGTG GGAAAGATGT GGCCCTTGAA
    GCTGAAACCA GCAGTATAAC ATCAATGACT CCTGAAGAAA CAAAGCCTCT CCCTGTACCC
    ACACATGTAG CTGTGACTGC AAGTTCTGCA AAAGGGCTCA TTGTGTATTG TGTTACCACA
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31952
    Clone ID Related Accession (Same CDS sequence) XM_012956500.1
    Accession Version XM_012956500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811954.1
    CDS354..5321
    Translation

    Target ORF information:

    RefSeq Version XM_012956500.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGGTA ATTTTTTTGC CGATGATGAG CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31953
    Clone ID Related Accession (Same CDS sequence) XM_012956506.1
    Accession Version XM_012956506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGTCTCT GAGTCAAGGC AGCACCAATG CTACGGTCCT CGACGTCGCC
    CAGTCGGGGG GACACAAAAA ACGTGTGCGT CGCAGCTCTT TCCTTAATGC CAAGAAACTG
    TACGAAGATG CTCAGATGGC TCGCAAGGTG AAGCAATACT TATCTGATCT AGATCTTGAA
    ATGGATGAAG AGAATCTTCA GACACTTTCT GTACAGTGTG AACCAGCAAC CAACACATTG
    CCAAAAAATC CATGTGAGAA GAGAGCAGGG AAGCCAGACA CATCGCCTGT GGCACCACGG
    GCAGCAGCAC AACACAAACA GCAACAGCAG CAGCAAACTA AGGTCAACCA GGCATTGCAA
    GTTCCTGCTG TGGCTCTTTA CCCATCTCGT AAGAAAGTGC CAGTCAAGGA TCTTCCACCC
    TTCGGCATCA ACTCTCCCCA AGCTTTAAAG AAAATTCTTT CGTTATCAGA AGAAGGAAGT
    TTGGAAAGGC ACAAGAAGCA AGCAGAGGAC ACCATATCAA ATGCATCCTC GCAGCTTTCA
    TCCCCTCCGA CATCCCCGCA GTGCTCTCCA AGGAAAGGTT ACACAATGGC AGCTAGTGGC
    ACAGTGGATA ACTTCTCTGA TTCAGGGCAC AGTGAAATCT CCTCCCGCTC CAGCATTGTC
    AGTAACTCTT CTTTTGACTG CGTGCACTTG CACGATGACC GGCGGCAGAG GCATTCCCTA
    AGCATAGTGG AGACCAATCT TGGTGTGGGA AGGGCTGACA GAAGAGCCAT TATAGATGCA
    GACCAGTGCA GCCTAGGCTC TTACGCGCCA GTGGCAGAGA CTCGGGGCCT CTATGCAGGT
    GCAACAGTTC TCTCCTCCCC CAGCATGGAG GAGCTTTCCC AGGATCAGGG GGATCGAGCT
    TCTTTAGATG CAGCAGATAG TGGCCGTGGA AGCTGGACTT CATGTTCCAG CGGATCCCAT
    GATAACATAC AAACTATCCC ACATCAGAGA AGCTGGGAGA CTCTTCCTTT TGGTCATGTT
    CATTTTGATA ACACAGCTGA TGGGTCAGGG TTTTGGGCCA CAGGTGGCCA CATGGACCAC
    GGGATATTTC CTGATCATAG CACAAAATAT GGCAGATACA GCCAAGGAAG AGAACCTTTG
    GATCAAGCAC AGTCAAGAGC CAGCTGGGCA TCATCAACAG GCTACTGGGG CGAGGACTCA
    GAAGGTGATA CCGGCACCAT TAAGAGAAGA GGTGGGAAAG ATGTGGCCCT TGAAGCTGAA
    ACCAGCAGTA TAACATCAAT GACTCCTGAA GAAACAAAGC CTCTCCCTGT ACCCACACAT
    GTAGCTGTGA CTGCAAGTTC TGCAAAAGGG CTCATTGTGT ATTGTGTTAC CACAGCAAGG
    AAAGAAGGCC GGTACAGAGA ACCGCCGCCC ACACCTCCAG GCTATGTAGG AATCCCCATA
    GCTGACTTTG CCGATGGACA CCCACCTCTA TCCAGAAAGC CACCAGATTA CAATGTGGCC
    TTGCAGCGTT CCAGAATGAT GGCTCGGAGA AGTGAAAGTG AAGGGTCAGC AGCAACCTCT
    CCGCCATCAC ATAATCAGAC TTCTGGCAGG ACTGCAAACA AACCCCAGTG GCATAAACCA
    AACGAGGCTG ACCCAAGGCT TGCTCTCTAT CAGGCACAAG GGGTTACAGA AAAAGATGAT
    GGTAATTTTT TTGCCGATGA TGAGCAAGTA TCTGCCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811960.1
    CDS354..5315
    Translation

    Target ORF information:

    RefSeq Version XM_012956506.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGTCTCT GAGTCAAGGC AGCACCAATG CTACGGTCCT CGACGTCGCC
    CAGTCGGGGG GACACAAAAA ACGTGTGCGT CGCAGCTCTT TCCTTAATGC CAAGAAACTG
    TACGAAGATG CTCAGATGGC TCGCAAGGTG AAGCAATACT TATCTGATCT AGATCTTGAA
    ATGGATGAAG AGAATCTTCA GACACTTTCT GTACAGTGTG AACCAGCAAC CAACACATTG
    CCAAAAAATC CATGTGAGAA GAGAGCAGGG AAGCCAGACA CATCGCCTGT GGCACCACGG
    GCAGCAGCAC AACACAAACA GCAACAGCAG CAGCAAACTA AGGTCAACCA GGCATTGCAA
    GTTCCTGCTG TGGCTCTTTA CCCATCTCGT AAGAAAGTGC CAGTCAAGGA TCTTCCACCC
    TTCGGCATCA ACTCTCCCCA AGCTTTAAAG AAAATTCTTT CGTTATCAGA AGAAGGAAGT
    TTGGAAAGGC ACAAGAAGCA AGCAGAGGAC ACCATATCAA ATGCATCCTC GCAGCTTTCA
    TCCCCTCCGA CATCCCCGCA GTGCTCTCCA AGGAAAGGTT ACACAATGGC AGCTAGTGGC
    ACAGTGGATA ACTTCTCTGA TTCAGGGCAC AGTGAAATCT CCTCCCGCTC CAGCATTGTC
    AGTAACTCTT CTTTTGACTG CGTGCACTTG CACGATGACC GGCGGCAGAG GCATTCCCTA
    AGCATAGTGG AGACCAATCT TGGTGTGGGA AGGGCTGACA GAAGAGCCAT TATAGATGCA
    GACCAGTGCA GCCTAGGCTC TTACGCGCCA GTGGCAGAGA CTCGGGGCCT CTATGCAGGT
    GCAACAGTTC TCTCCTCCCC CAGCATGGAG GAGCTTTCCC AGGATCAGGG GGATCGAGCT
    TCTTTAGATG CAGCAGATAG TGGCCGTGGA AGCTGGACTT CATGTTCCAG CGGATCCCAT
    GATAACATAC AAACTATCCC ACATCAGAGA AGCTGGGAGA CTCTTCCTTT TGGTCATGTT
    CATTTTGATA ACACAGCTGA TGGGTCAGGG TTTTGGGCCA CAGGTGGCCA CATGGACCAC
    GGGATATTTC CTGATCATAG CACAAAATAT GGCAGATACA GCCAAGGAAG AGAACCTTTG
    GATCAAGCAC AGTCAAGAGC CAGCTGGGCA TCATCAACAG GCTACTGGGG CGAGGACTCA
    GAAGGTGATA CCGGCACCAT TAAGAGAAGA GGTGGGAAAG ATGTGGCCCT TGAAGCTGAA
    ACCAGCAGTA TAACATCAAT GACTCCTGAA GAAACAAAGC CTCTCCCTGT ACCCACACAT
    GTAGCTGTGA CTGCAAGTTC TGCAAAAGGG CTCATTGTGT ATTGTGTTAC CACAGCAAGG
    AAAGAAGGCC GGTACAGAGA ACCGCCGCCC ACACCTCCAG GCTATGTAGG AATCCCCATA
    GCTGACTTTG CCGATGGACA CCCACCTCTA TCCAGAAAGC CACCAGATTA CAATGTGGCC
    TTGCAGCGTT CCAGAATGAT GGCTCGGAGA AGTGAAAGTG AAGGGTCAGC AGCAACCTCT
    CCGCCATCAC ATAATCAGAC TTCTGGCAGG ACTGCAAACA AACCCCAGTG GCATAAACCA
    AACGAGGCTG ACCCAAGGCT TGCTCTCTAT CAGGCACAAG GGGTTACAGA AAAAGATGAT
    GGTAATTTTT TTGCCGATGA TGAGCAAGTA TCTGCCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31954
    Clone ID Related Accession (Same CDS sequence) XM_012956508.1
    Accession Version XM_012956508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGCCG ATGATGAGCA AGTATCTGCC GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811962.1
    CDS354..5309
    Translation

    Target ORF information:

    RefSeq Version XM_012956508.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ATGTGGATAA CAGTTTCAGG CAGGCTGTGA TGAAGCCCCC GGCTGACAGA 
    AGCGCACAGG ACCTGGAGAT TGTTTATTCC TACCTTCATG GAATGGAAGC ACTGTCTAAC
    CTTCGGGAAC ATCAGCTTAG ACTGATGTGT GAAACTGTCC GTTACGAAAG GCATGAAGCT
    AATGAAGTCT TGTATTACCC TGACGACATT GGTACCTGTT GGTATATCCT TTTGTCTGGT
    TCTGTGTTCA TCAAAGAGTC CATGTTTCTT CCAAGGAGCA GCTTTGGCAA ACGTTCTGCT
    GGAAGCTTAA GGCGTGGCTG TGAATGCATC GTATTGGAGC CTTCCGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAGCGCCAGG CATCTCACCG GCAGTCTCGC
    CGGAGGTTCC GCAAGATCAA TCAGAAAGGC GAGAGGCAAA CCATTATTGA TACAGTGGAA
    TCCTATCCTG TTGGAAAACC ACCTTTGCCA AGAGGGTATC ATACGGAAGG CTCTAAGCCT
    CAGCTCCCTG CTGATTTCAC TAAACTGCAC ATCACAGACA GTCTACATCC ACAGGTGACG
    CACGTGTCCT CTAGTCATTC AGGATGTAGC ATCACTAGCG ATTCTGGGAG CAGCAGCTTG
    TCTGACATCT ACCAGGCCAC AGAAAGTGAG CCAGGAGATA TGGACCTGAG TGGGCTGCCA
    GAGACCGCTG TAGACTCAGA AGATGACGAT GATGAAGAGG ATATTGAAAG AGCTTCTGAC
    CCTTTAATGA GCAGGGACAT TGTCAGAGAC TGTCTGGAGA AGGATCCAAT TGACAGGACT
    GACGACGATA TTGAACAACT TCTGGAGTTC ATGCACCAGT TGCCAGCATT TGCCAACATG
    ACAATGTCTG TAAGACGAGA GCTCTGTGCA GTCATGGTGT TCGCTGTGGT CGAGCGGGCA
    GGAACTGTTG TACTAAATGA TGGGGAGGAG CTCGATTCCT GGTCAGTTAT TTTAAATGGC
    TCAGTAGAAG TGACATATCC TGATGGCAGG ACAGAAATAC TTTGCATGGG GAACAGCTTT
    GGTGTGTCGC CAACCATGGA GAAGGAATAC ATGAAGGGAG TCATGAGAAC CAAAGTGGAT
    GACTGCCAGT TTGTGTGCAT CGCCCAGCAA GATTACTGCC GCATCCTCAA TCAGGTGGAA
    AAGAACATGC AGAAAGTGGA AGAGGAGGGT GAGATTGTGA TGGTGAAAGA GCACAGAGAG
    CTTGATCGCA CCGGAACCAG GAAAGGTCAT ATTGTCATCA AGGGAACATC GGAACGCCTA
    ACCATGCATC TTGTGGAAGA ACATTCTGTG GTTGACCCAA CATATATTGA AGATTTTCTC
    CTCACTTACA GAACTTTCCT TTCTAGTCCG ATGGAAGTGG GAAAGAAACT ACTGGAGTGG
    TTTAATGACC CTAGTCTCAG GGACAAGGTT ACACGAGTAG TTTTGCTCTG GGTAAATAAT
    CACTTTAATG ACTTTGAAGG GGACCCAGCC ATGACTCGAT TTTTGGAAGA ATTTGAGAAC
    AATTTGGAAC GTGAGAAAAT GGGTGGCCAC CTAAGGCTAT TAAATATTGC ATGTGCTGCC
    AAAGCAAAAA GAAGATTGAT AACTCTAACA AAGCCCTCCC GAGAAGCCCC CTTGCCTTTT
    AACCTGCTGG GAGGGTCAGA CAAGGGATTT GGCATCTTTG TAGACAGCGT GGATCCAGGA
    AGCAAAGCAG CAGAGACTGG CTTAAAGCGA GGGGATCAGA TTTTGGAGGT AAATGGGCAG
    AACTTTGAAA ACATTCAACT TACCAAGGCT TTGGAAATCC TAAGGAACAA TACTCACTTG
    TCTCTCACTG TGAAAACAAA TTTATTTGTG TTTAAGGAAC TTCTGGCCAG AATGTTAGAG
    GAAAAGAGAA ATAGTGCCCC ACACTTGCCC AAAATTGGAG ACATCAAAAA AGCCAGCCGC
    TATTCTATTC CAGACCTTGC CGTTGATGTT GAACAAGTTA TTGGACTTGA AAAAGTGAGC
    AAAAAGGGCA AAGCCAATAC TGTCGGAGGA CGAAACAAGC TGAAAAAGAT TCTTGACAAG
    ACACGGATCA GTATCCTGCC TCAAAAGCCT TACAATGATA TTGGAATCGG TCAGTCTCAA
    GATGACAGCA TTGTCGGTCT CCGACAGACC AAGCACATGC CGGCTACACT ACCTGTCAGT
    GGGACATTAT CTTCCAGCAA TCCGGATTTA CTGCAGTCCC ATCACCGCAT CTTAGACTTT
    AATACTACAC CAGAACAACC GGACCAGGTG CTGAGAGTTT TCAAGGCCGA TCAGCAGAGC
    CGTTACATAA TGATAAGCAA GGACACCACC GCTAAGGAAG TGGTCATCCA GGCAATCAGA
    GAATTTGCAT TAACTGCGGT ACCTGATGCC TACTCATTGT GTGAAGTGTC TGTCACCCCT
    GAAGGTGTCA TCAAACAAAG GCGGCTTCCT GATCAGCTTT CCAAGCTGGC TGACAGAATA
    CAACTCAGTG GCAGGTATTA CCTAAAAAAC AACATGGAAA CTGAAACACT ATGCTCAGAT
    GAAGATGCCC AAGATCTTCT AAGGGAAAGT CAGATCTCTT TGCTACAGCT CAGCACCATT
    GAAGTGGCAA CACAGCTGTC TATGAGAAAT TTTGAGCTAT TTAGGAACAT TGAGCCCACC
    GAGTACATTG ATGACTTGTT CAAACTGAAA ATGAAAACTG GCTGCACCAA CCTCAAAAAC
    TTCGAAGAAG TCATTAACCA AGAGACCTTC TGGGTAGCCT CAGAAATTCT AAGGGAAACT
    AACCAGCTAA AACGGATGAA GATCATCAAG CATTTTATCA AGATCGCACT ACATTGTAGA
    GAGTGCAAGA ACTTCAACTC CATGTTTGCT ATCATTAGTG GCTTAAATTT GGCACCAGTT
    TCAAGACTCC GAACAACTTG GGAAAAACTC CCAAGCAAAT ATGAGAAACT CTTTCAAGAC
    CTCCAGGACC TCTTTGACCC TTCCAGGAAC ATGGCTAAAT ATCGGAATGT GCTGAATAGT
    CAGAACCTTC AGCCTCCCAT TATACCTTTA TTTCCTGTCA TTAAGAAAGA CCTCACCTTC
    CTTCATGAGG GCAATGACTC CAAAGTAGAC GGATTAGTTA ACTTTGAGAA GCTTAGGATG
    ATTGCAAAAG AAATCCGCCA TGTGGGAAGA ATGGCTTCAG TAAATATGGA TCCAGCTCTT
    ATGTTCCGAA CCAGGAAGAA GAAGTGGAGG AGTCTGGGGT CTCTGAGTCA AGGCAGCACC
    AATGCTACGG TCCTCGACGT CGCCCAGTCG GGGGGACACA AAAAACGTGT GCGTCGCAGC
    TCTTTCCTTA ATGCCAAGAA ACTGTACGAA GATGCTCAGA TGGCTCGCAA GGTGAAGCAA
    TACTTATCTG ATCTAGATCT TGAAATGGAT GAAGAGAATC TTCAGACACT TTCTGTACAG
    TGTGAACCAG CAACCAACAC ATTGCCAAAA AATCCATGTG AGAAGAGAGC AGGGAAGCCA
    GACACATCGC CTGTGGCACC ACGGGCAGCA GCACAACACA AACAGCAACA GCAGCAGCAA
    ACTAAGGTCA ACCAGGCATT GCAAGTTCCT GCTGTGGCTC TTTACCCATC TCGTAAGAAA
    GTGCCAGTCA AGGATCTTCC ACCCTTCGGC ATCAACTCTC CCCAAGCTTT AAAGAAAATT
    CTTTCGTTAT CAGAAGAAGG AAGTTTGGAA AGGCACAAGA AGCAAGCAGA GGACACCATA
    TCAAATGCAT CCTCGCAGCT TTCATCCCCT CCGACATCCC CGCAGTGCTC TCCAAGGAAA
    GGTTACACAA TGGCAGCTAG TGGCACAGTG GATAACTTCT CTGATTCAGG GCACAGTGAA
    ATCTCCTCCC GCTCCAGCAT TGTCAGTAAC TCTTCTTTTG ACTGCGTGCA CTTGCACGAT
    GACCGGCGGC AGAGGCATTC CCTAAGCATA GTGGAGACCA ATCTTGGTGT GGGAAGGGCT
    GACAGAAGAG CCATTATAGA TGCAGACCAG TGCAGCCTAG GCTCTTACGC GCCAGTGGCA
    GAGACTCGGG GCCTCTATGC AGGTGCAACA GTTCTCTCCT CCCCCAGCAT GGAGGAGCTT
    TCCCAGGATC AGGGGGATCG AGCTTCTTTA GATGCAGCAG ATAGTGGCCG TGGAAGCTGG
    ACTTCATGTT CCAGCGGATC CCATGATAAC ATACAAACTA TCCCACATCA GAGAAGCTGG
    GAGACTCTTC CTTTTGGTCA TGTTCATTTT GATAACACAG CTGATGGGTC AGGGTTTTGG
    GCCACAGGTG GCCACATGGA CCACGGGATA TTTCCTGATC ATAGCACAAA ATATGGCAGA
    TACAGCCAAG GAAGAGAACC TTTGGATCAA GCACAGTCAA GAGCCAGCTG GGCATCATCA
    ACAGGCTACT GGGGCGAGGA CTCAGAAGGT GATACCGGCA CCATTAAGAG AAGAGGTGGG
    AAAGATGTGG CCCTTGAAGC TGAAACCAGC AGTATAACAT CAATGACTCC TGAAGAAACA
    AAGCCTCTCC CTGTACCCAC ACATGTAGCT GTGACTGCAA GTTCTGCAAA AGGGCTCATT
    GCAAGGAAAG AAGGCCGGTA CAGAGAACCG CCGCCCACAC CTCCAGGCTA TGTAGGAATC
    CCCATAGCTG ACTTTGCCGA TGGACACCCA CCTCTATCCA GAAAGCCACC AGATTACAAT
    GTGGCCTTGC AGCGTTCCAG AATGATGGCT CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA
    ACCTCTCCGC CATCACATAA TCAGACTTCT GGCAGGACTG CAAACAAACC CCAGTGGCAT
    AAACCAAACG AGGCTGACCC AAGGCTTGCT CTCTATCAGG CACAAGGGGT TACAGAAAAA
    GATGATGCCG ATGATGAGCA AGTATCTGCC GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31955
    Clone ID Related Accession (Same CDS sequence) XM_012956514.2
    Accession Version XM_012956514.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAGCGCCA GGCATCTCAC 
    CGGCAGTCTC GCCGGAGGTT CCGCAAGATC AATCAGAAAG GCGAGAGGCA AACCATTATT
    GATACAGTGG AATCCTATCC TGTTGGAAAA CCACCTTTGC CAAGAGGGTA TCATACGGAA
    GGCTCTAAGC CTCAGCTCCC TGCTGATTTC ACTAAACTGC ACATCACAGA CAGTCTACAT
    CCACAGGTGA CGCACGTGTC CTCTAGTCAT TCAGGATGTA GCATCACTAG CGATTCTGGG
    AGCAGCAGCT TGTCTGACAT CTACCAGGCC ACAGAAAGTG AGCCAGGAGA TATGGACCTG
    AGTGGGCTGC CAGAGACCGC TGTAGACTCA GAAGATGACG ATGATGAAGA GGATATTGAA
    AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC ATTGTCAGAG ACTGTCTGGA GAAGGATCCA
    ATTGACAGGA CTGACGACGA TATTGAACAA CTTCTGGAGT TCATGCACCA GTTGCCAGCA
    TTTGCCAACA TGACAATGTC TGTAAGACGA GAGCTCTGTG CAGTCATGGT GTTCGCTGTG
    GTCGAGCGGG CAGGAACTGT TGTACTAAAT GATGGGGAGG AGCTCGATTC CTGGTCAGTT
    ATTTTAAATG GCTCAGTAGA AGTGACATAT CCTGATGGCA GGACAGAAAT ACTTTGCATG
    GGGAACAGCT TTGGTGTGTC GCCAACCATG GAGAAGGAAT ACATGAAGGG AGTCATGAGA
    ACCAAAGTGG ATGACTGCCA GTTTGTGTGC ATCGCCCAGC AAGATTACTG CCGCATCCTC
    AATCAGGTGG AAAAGAACAT GCAGAAAGTG GAAGAGGAGG GTGAGATTGT GATGGTGAAA
    GAGCACAGAG AGCTTGATCG CACCGGAACC AGGAAAGGTC ATATTGTCAT CAAGGGAACA
    TCGGAACGCC TAACCATGCA TCTTGTGGAA GAACATTCTG TGGTTGACCC AACATATATT
    GAAGATTTTC TCCTCACTTA CAGAACTTTC CTTTCTAGTC CGATGGAAGT GGGAAAGAAA
    CTACTGGAGT GGTTTAATGA CCCTAGTCTC AGGGACAAGG TTACACGAGT AGTTTTGCTC
    TGGGTAAATA ATCACTTTAA TGACTTTGAA GGGGACCCAG CCATGACTCG ATTTTTGGAA
    GAATTTGAGA ACAATTTGGA ACGTGAGAAA ATGGGTGGCC ACCTAAGGCT ATTAAATATT
    GCATGTGCTG CCAAAGCAAA AAGAAGATTG ATAACTCTAA CAAAGCCCTC CCGAGAAGCC
    CCCTTGCCTT TTAACCTGCT GGGAGGGTCA GACAAGGGAT TTGGCATCTT TGTAGACAGC
    GTGGATCCAG GAAGCAAAGC AGCAGAGACT GGCTTAAAGC GAGGGGATCA GATTTTGGAG
    GTAAATGGGC AGAACTTTGA AAACATTCAA CTTACCAAGG CTTTGGAAAT CCTAAGGAAC
    AATACTCACT TGTCTCTCAC TGTGAAAACA AATTTATTTG TGTTTAAGGA ACTTCTGGCC
    AGAATGTTAG AGGAAAAGAG AAATAGTGCC CCACACTTGC CCAAAATTGG AGACATCAAA
    AAAGCCAGCC GCTATTCTAT TCCAGACCTT GCCGTTGATG TTGAACAAGT TATTGGACTT
    GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT ACTGTCGGAG GACGAAACAA GCTGAAAAAG
    ATTCTTGACA AGACACGGAT CAGTATCCTG CCTCAAAAGC CTTACAATGA TATTGGAATC
    GGTCAGTCTC AAGATGACAG CATTGTCGGT CTCCGACAGA CCAAGCACAT GCCGGCTACA
    CTACCTGTCA GTGGGACATT ATCTTCCAGC AATCCGGATT TACTGCAGTC CCATCACCGC
    ATCTTAGACT TTAATACTAC ACCAGAACAA CCGGACCAGG TGCTGAGAGT TTTCAAGGCC
    GATCAGCAGA GCCGTTACAT AATGATAAGC AAGGACACCA CCGCTAAGGA AGTGGTCATC
    CAGGCAATCA GAGAATTTGC ATTAACTGCG GTACCTGATG CCTACTCATT GTGTGAAGTG
    TCTGTCACCC CTGAAGGTGT CATCAAACAA AGGCGGCTTC CTGATCAGCT TTCCAAGCTG
    GCTGACAGAA TACAACTCAG TGGCAGGTAT TACCTAAAAA ACAACATGGA AACTGAAACA
    CTATGCTCAG ATGAAGATGC CCAAGATCTT CTAAGGGAAA GTCAGATCTC TTTGCTACAG
    CTCAGCACCA TTGAAGTGGC AACACAGCTG TCTATGAGAA ATTTTGAGCT ATTTAGGAAC
    ATTGAGCCCA CCGAGTACAT TGATGACTTG TTCAAACTGA AAATGAAAAC TGGCTGCACC
    AACCTCAAAA ACTTCGAAGA AGTCATTAAC CAAGAGACCT TCTGGGTAGC CTCAGAAATT
    CTAAGGGAAA CTAACCAGCT AAAACGGATG AAGATCATCA AGCATTTTAT CAAGATCGCA
    CTACATTGTA GAGAGTGCAA GAACTTCAAC TCCATGTTTG CTATCATTAG TGGCTTAAAT
    TTGGCACCAG TTTCAAGACT CCGAACAACT TGGGAAAAAC TCCCAAGCAA ATATGAGAAA
    CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC CCTTCCAGGA ACATGGCTAA ATATCGGAAT
    GTGCTGAATA GTCAGAACCT TCAGCCTCCC ATTATACCTT TATTTCCTGT CATTAAGAAA
    GACCTCACCT TCCTTCATGA GGGCAATGAC TCCAAAGTAG ACGGATTAGT TAACTTTGAG
    AAGCTTAGGA TGATTGCAAA AGAAATCCGC CATGTGGGAA GAATGGCTTC AGTAAATATG
    GATCCAGCTC TTATGTTCCG AACCAGGAAG AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT
    CAAGGCAGCA CCAATGCTAC GGTCCTCGAC GTCGCCCAGT CGGGGGGACA CAAAAAACGT
    GTGCGTCGCA GCTCTTTCCT TAATGCCAAG AAACTGTACG AAGATGCTCA GATGGCTCGC
    AAGGTGAAGC AATACTTATC TGATCTAGAT CTTGAAATGG ATGAAGAGAA TCTTCAGACA
    CTTTCTGTAC AGTGTGAACC AGCAACCAAC ACATTGCCAA AAAATCCATG TGAGAAGAGA
    GCAGGGAAGC CAGACACATC GCCTGTGGCA CCACGGGCAG CAGCACAACA CAAACAGCAA
    CAGCAGCAGC AAACTAAGGT CAACCAGGCA TTGCAAGTTC CTGCTGTGGC TCTTTACCCA
    TCTCGTAAGA AAGTGCCAGT CAAGGATCTT CCACCCTTCG GCATCAACTC TCCCCAAGCT
    TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA GGAAGTTTGG AAAGGCACAA GAAGCAAGCA
    GAGGACACCA TATCAAATGC ATCCTCGCAG CTTTCATCCC CTCCGACATC CCCGCAGTGC
    TCTCCAAGGA AAGGTTACAC AATGGCAGCT AGTGGCACAG TGGATAACTT CTCTGATTCA
    GGGCACAGTG AAATCTCCTC CCGCTCCAGC ATTGTCAGTA ACTCTTCTTT TGACTGCGTG
    CACTTGCACG ATGACCGGCG GCAGAGGCAT TCCCTAAGCA TAGTGGAGAC CAATCTTGGT
    GTGGGAAGGG CTGACAGAAG AGCCATTATA GATGCAGACC AGTGCAGCCT AGGCTCTTAC
    GCGCCAGTGG CAGAGACTCG GGGCCTCTAT GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC
    ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT CGAGCTTCTT TAGATGCAGC AGATAGTGGC
    CGTGGAAGCT GGACTTCATG TTCCAGCGGA TCCCATGATA ACATACAAAC TATCCCACAT
    CAGAGAAGCT GGGAGACTCT TCCTTTTGGT CATGTTCATT TTGATAACAC AGCTGATGGG
    TCAGGGTTTT GGGCCACAGG TGGCCACATG GACCACGGGA TATTTCCTGA TCATAGCACA
    AAATATGGCA GATACAGCCA AGGAAGAGAA CCTTTGGATC AAGCACAGTC AAGAGCCAGC
    TGGGCATCAT CAACAGGCTA CTGGGGCGAG GACTCAGAAG GTGATACCGG CACCATTAAG
    AGAAGAGGTG GGAAAGATGT GGCCCTTGAA GCTGAAACCA GCAGTATAAC ATCAATGACT
    CCTGAAGAAA CAAAGCCTCT CCCTGTACCC ACACATGTAG CTGTGACTGC AAGTTCTGCA
    AAAGGGCTCA TTGTGTATTG TGTTACCACA GCAAGGAAAG AAGGCCGGTA CAGAGAACCG
    CCGCCCACAC CTCCAGGCTA TGTAGGAATC CCCATAGCTG ACTTTGCCGA TGGACACCCA
    CCTCTATCCA GAAAGCCACC AGATTACAAT GTGGCCTTGC AGCGTTCCAG AATGATGGCT
    CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA ACCTCTCCGC CATCACATAA TCAGACTTCT
    GGCAGGACTG CAAACAAACC CCAGTGGCAT AAACCAAACG AGGCTGACCC AAGGCTTGCT
    CTCTATCAGG CACAAGGGGT TACAGAAAAA GATGATGGTA ATTTTTTTGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811968.1
    CDS626..5263
    Translation

    Target ORF information:

    RefSeq Version XM_012956514.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956514.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAGCGCCA GGCATCTCAC 
    CGGCAGTCTC GCCGGAGGTT CCGCAAGATC AATCAGAAAG GCGAGAGGCA AACCATTATT
    GATACAGTGG AATCCTATCC TGTTGGAAAA CCACCTTTGC CAAGAGGGTA TCATACGGAA
    GGCTCTAAGC CTCAGCTCCC TGCTGATTTC ACTAAACTGC ACATCACAGA CAGTCTACAT
    CCACAGGTGA CGCACGTGTC CTCTAGTCAT TCAGGATGTA GCATCACTAG CGATTCTGGG
    AGCAGCAGCT TGTCTGACAT CTACCAGGCC ACAGAAAGTG AGCCAGGAGA TATGGACCTG
    AGTGGGCTGC CAGAGACCGC TGTAGACTCA GAAGATGACG ATGATGAAGA GGATATTGAA
    AGAGCTTCTG ACCCTTTAAT GAGCAGGGAC ATTGTCAGAG ACTGTCTGGA GAAGGATCCA
    ATTGACAGGA CTGACGACGA TATTGAACAA CTTCTGGAGT TCATGCACCA GTTGCCAGCA
    TTTGCCAACA TGACAATGTC TGTAAGACGA GAGCTCTGTG CAGTCATGGT GTTCGCTGTG
    GTCGAGCGGG CAGGAACTGT TGTACTAAAT GATGGGGAGG AGCTCGATTC CTGGTCAGTT
    ATTTTAAATG GCTCAGTAGA AGTGACATAT CCTGATGGCA GGACAGAAAT ACTTTGCATG
    GGGAACAGCT TTGGTGTGTC GCCAACCATG GAGAAGGAAT ACATGAAGGG AGTCATGAGA
    ACCAAAGTGG ATGACTGCCA GTTTGTGTGC ATCGCCCAGC AAGATTACTG CCGCATCCTC
    AATCAGGTGG AAAAGAACAT GCAGAAAGTG GAAGAGGAGG GTGAGATTGT GATGGTGAAA
    GAGCACAGAG AGCTTGATCG CACCGGAACC AGGAAAGGTC ATATTGTCAT CAAGGGAACA
    TCGGAACGCC TAACCATGCA TCTTGTGGAA GAACATTCTG TGGTTGACCC AACATATATT
    GAAGATTTTC TCCTCACTTA CAGAACTTTC CTTTCTAGTC CGATGGAAGT GGGAAAGAAA
    CTACTGGAGT GGTTTAATGA CCCTAGTCTC AGGGACAAGG TTACACGAGT AGTTTTGCTC
    TGGGTAAATA ATCACTTTAA TGACTTTGAA GGGGACCCAG CCATGACTCG ATTTTTGGAA
    GAATTTGAGA ACAATTTGGA ACGTGAGAAA ATGGGTGGCC ACCTAAGGCT ATTAAATATT
    GCATGTGCTG CCAAAGCAAA AAGAAGATTG ATAACTCTAA CAAAGCCCTC CCGAGAAGCC
    CCCTTGCCTT TTAACCTGCT GGGAGGGTCA GACAAGGGAT TTGGCATCTT TGTAGACAGC
    GTGGATCCAG GAAGCAAAGC AGCAGAGACT GGCTTAAAGC GAGGGGATCA GATTTTGGAG
    GTAAATGGGC AGAACTTTGA AAACATTCAA CTTACCAAGG CTTTGGAAAT CCTAAGGAAC
    AATACTCACT TGTCTCTCAC TGTGAAAACA AATTTATTTG TGTTTAAGGA ACTTCTGGCC
    AGAATGTTAG AGGAAAAGAG AAATAGTGCC CCACACTTGC CCAAAATTGG AGACATCAAA
    AAAGCCAGCC GCTATTCTAT TCCAGACCTT GCCGTTGATG TTGAACAAGT TATTGGACTT
    GAAAAAGTGA GCAAAAAGGG CAAAGCCAAT ACTGTCGGAG GACGAAACAA GCTGAAAAAG
    ATTCTTGACA AGACACGGAT CAGTATCCTG CCTCAAAAGC CTTACAATGA TATTGGAATC
    GGTCAGTCTC AAGATGACAG CATTGTCGGT CTCCGACAGA CCAAGCACAT GCCGGCTACA
    CTACCTGTCA GTGGGACATT ATCTTCCAGC AATCCGGATT TACTGCAGTC CCATCACCGC
    ATCTTAGACT TTAATACTAC ACCAGAACAA CCGGACCAGG TGCTGAGAGT TTTCAAGGCC
    GATCAGCAGA GCCGTTACAT AATGATAAGC AAGGACACCA CCGCTAAGGA AGTGGTCATC
    CAGGCAATCA GAGAATTTGC ATTAACTGCG GTACCTGATG CCTACTCATT GTGTGAAGTG
    TCTGTCACCC CTGAAGGTGT CATCAAACAA AGGCGGCTTC CTGATCAGCT TTCCAAGCTG
    GCTGACAGAA TACAACTCAG TGGCAGGTAT TACCTAAAAA ACAACATGGA AACTGAAACA
    CTATGCTCAG ATGAAGATGC CCAAGATCTT CTAAGGGAAA GTCAGATCTC TTTGCTACAG
    CTCAGCACCA TTGAAGTGGC AACACAGCTG TCTATGAGAA ATTTTGAGCT ATTTAGGAAC
    ATTGAGCCCA CCGAGTACAT TGATGACTTG TTCAAACTGA AAATGAAAAC TGGCTGCACC
    AACCTCAAAA ACTTCGAAGA AGTCATTAAC CAAGAGACCT TCTGGGTAGC CTCAGAAATT
    CTAAGGGAAA CTAACCAGCT AAAACGGATG AAGATCATCA AGCATTTTAT CAAGATCGCA
    CTACATTGTA GAGAGTGCAA GAACTTCAAC TCCATGTTTG CTATCATTAG TGGCTTAAAT
    TTGGCACCAG TTTCAAGACT CCGAACAACT TGGGAAAAAC TCCCAAGCAA ATATGAGAAA
    CTCTTTCAAG ACCTCCAGGA CCTCTTTGAC CCTTCCAGGA ACATGGCTAA ATATCGGAAT
    GTGCTGAATA GTCAGAACCT TCAGCCTCCC ATTATACCTT TATTTCCTGT CATTAAGAAA
    GACCTCACCT TCCTTCATGA GGGCAATGAC TCCAAAGTAG ACGGATTAGT TAACTTTGAG
    AAGCTTAGGA TGATTGCAAA AGAAATCCGC CATGTGGGAA GAATGGCTTC AGTAAATATG
    GATCCAGCTC TTATGTTCCG AACCAGGAAG AAGAAGTGGA GGAGTCTGGG GTCTCTGAGT
    CAAGGCAGCA CCAATGCTAC GGTCCTCGAC GTCGCCCAGT CGGGGGGACA CAAAAAACGT
    GTGCGTCGCA GCTCTTTCCT TAATGCCAAG AAACTGTACG AAGATGCTCA GATGGCTCGC
    AAGGTGAAGC AATACTTATC TGATCTAGAT CTTGAAATGG ATGAAGAGAA TCTTCAGACA
    CTTTCTGTAC AGTGTGAACC AGCAACCAAC ACATTGCCAA AAAATCCATG TGAGAAGAGA
    GCAGGGAAGC CAGACACATC GCCTGTGGCA CCACGGGCAG CAGCACAACA CAAACAGCAA
    CAGCAGCAGC AAACTAAGGT CAACCAGGCA TTGCAAGTTC CTGCTGTGGC TCTTTACCCA
    TCTCGTAAGA AAGTGCCAGT CAAGGATCTT CCACCCTTCG GCATCAACTC TCCCCAAGCT
    TTAAAGAAAA TTCTTTCGTT ATCAGAAGAA GGAAGTTTGG AAAGGCACAA GAAGCAAGCA
    GAGGACACCA TATCAAATGC ATCCTCGCAG CTTTCATCCC CTCCGACATC CCCGCAGTGC
    TCTCCAAGGA AAGGTTACAC AATGGCAGCT AGTGGCACAG TGGATAACTT CTCTGATTCA
    GGGCACAGTG AAATCTCCTC CCGCTCCAGC ATTGTCAGTA ACTCTTCTTT TGACTGCGTG
    CACTTGCACG ATGACCGGCG GCAGAGGCAT TCCCTAAGCA TAGTGGAGAC CAATCTTGGT
    GTGGGAAGGG CTGACAGAAG AGCCATTATA GATGCAGACC AGTGCAGCCT AGGCTCTTAC
    GCGCCAGTGG CAGAGACTCG GGGCCTCTAT GCAGGTGCAA CAGTTCTCTC CTCCCCCAGC
    ATGGAGGAGC TTTCCCAGGA TCAGGGGGAT CGAGCTTCTT TAGATGCAGC AGATAGTGGC
    CGTGGAAGCT GGACTTCATG TTCCAGCGGA TCCCATGATA ACATACAAAC TATCCCACAT
    CAGAGAAGCT GGGAGACTCT TCCTTTTGGT CATGTTCATT TTGATAACAC AGCTGATGGG
    TCAGGGTTTT GGGCCACAGG TGGCCACATG GACCACGGGA TATTTCCTGA TCATAGCACA
    AAATATGGCA GATACAGCCA AGGAAGAGAA CCTTTGGATC AAGCACAGTC AAGAGCCAGC
    TGGGCATCAT CAACAGGCTA CTGGGGCGAG GACTCAGAAG GTGATACCGG CACCATTAAG
    AGAAGAGGTG GGAAAGATGT GGCCCTTGAA GCTGAAACCA GCAGTATAAC ATCAATGACT
    CCTGAAGAAA CAAAGCCTCT CCCTGTACCC ACACATGTAG CTGTGACTGC AAGTTCTGCA
    AAAGGGCTCA TTGTGTATTG TGTTACCACA GCAAGGAAAG AAGGCCGGTA CAGAGAACCG
    CCGCCCACAC CTCCAGGCTA TGTAGGAATC CCCATAGCTG ACTTTGCCGA TGGACACCCA
    CCTCTATCCA GAAAGCCACC AGATTACAAT GTGGCCTTGC AGCGTTCCAG AATGATGGCT
    CGGAGAAGTG AAAGTGAAGG GTCAGCAGCA ACCTCTCCGC CATCACATAA TCAGACTTCT
    GGCAGGACTG CAAACAAACC CCAGTGGCAT AAACCAAACG AGGCTGACCC AAGGCTTGCT
    CTCTATCAGG CACAAGGGGT TACAGAAAAA GATGATGGTA ATTTTTTTGC CGATGATGAG
    CAAGTATCTG CCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31956
    Clone ID Related Accession (Same CDS sequence) XM_012956519.2
    Accession Version XM_012956519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAACCTC TTGCATTCCC ATCTAGTCAT GGAGTTATGG GCCAGCAGGA GAAGCATTCT 
    CTCCCTGCTG ATTTCACTAA ACTGCACATC ACAGACAGTC TACATCCACA GGTGACGCAC
    GTGTCCTCTA GTCATTCAGG ATGTAGCATC ACTAGCGATT CTGGGAGCAG CAGCTTGTCT
    GACATCTACC AGGCCACAGA AAGTGAGCCA GGAGATATGG ACCTGAGTGG GCTGCCAGAG
    ACCGCTGTAG ACTCAGAAGA TGACGATGAT GAAGAGGATA TTGAAAGAGC TTCTGACCCT
    TTAATGAGCA GGGACATTGT CAGAGACTGT CTGGAGAAGG ATCCAATTGA CAGGACTGAC
    GACGATATTG AACAACTTCT GGAGTTCATG CACCAGTTGC CAGCATTTGC CAACATGACA
    ATGTCTGTAA GACGAGAGCT CTGTGCAGTC ATGGTGTTCG CTGTGGTCGA GCGGGCAGGA
    ACTGTTGTAC TAAATGATGG GGAGGAGCTC GATTCCTGGT CAGTTATTTT AAATGGCTCA
    GTAGAAGTGA CATATCCTGA TGGCAGGACA GAAATACTTT GCATGGGGAA CAGCTTTGGT
    GTGTCGCCAA CCATGGAGAA GGAATACATG AAGGGAGTCA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TGTGCATCGC CCAGCAAGAT TACTGCCGCA TCCTCAATCA GGTGGAAAAG
    AACATGCAGA AAGTGGAAGA GGAGGGTGAG ATTGTGATGG TGAAAGAGCA CAGAGAGCTT
    GATCGCACCG GAACCAGGAA AGGTCATATT GTCATCAAGG GAACATCGGA ACGCCTAACC
    ATGCATCTTG TGGAAGAACA TTCTGTGGTT GACCCAACAT ATATTGAAGA TTTTCTCCTC
    ACTTACAGAA CTTTCCTTTC TAGTCCGATG GAAGTGGGAA AGAAACTACT GGAGTGGTTT
    AATGACCCTA GTCTCAGGGA CAAGGTTACA CGAGTAGTTT TGCTCTGGGT AAATAATCAC
    TTTAATGACT TTGAAGGGGA CCCAGCCATG ACTCGATTTT TGGAAGAATT TGAGAACAAT
    TTGGAACGTG AGAAAATGGG TGGCCACCTA AGGCTATTAA ATATTGCATG TGCTGCCAAA
    GCAAAAAGAA GATTGATAAC TCTAACAAAG CCCTCCCGAG AAGCCCCCTT GCCTTTTAAC
    CTGCTGGGAG GGTCAGACAA GGGATTTGGC ATCTTTGTAG ACAGCGTGGA TCCAGGAAGC
    AAAGCAGCAG AGACTGGCTT AAAGCGAGGG GATCAGATTT TGGAGGTAAA TGGGCAGAAC
    TTTGAAAACA TTCAACTTAC CAAGGCTTTG GAAATCCTAA GGAACAATAC TCACTTGTCT
    CTCACTGTGA AAACAAATTT ATTTGTGTTT AAGGAACTTC TGGCCAGAAT GTTAGAGGAA
    AAGAGAAATA GTGCCCCACA CTTGCCCAAA ATTGGAGACA TCAAAAAAGC CAGCCGCTAT
    TCTATTCCAG ACCTTGCCGT TGATGTTGAA CAAGTTATTG GACTTGAAAA AGTGAGCAAA
    AAGGGCAAAG CCAATACTGT CGGAGGACGA AACAAGCTGA AAAAGATTCT TGACAAGACA
    CGGATCAGTA TCCTGCCTCA AAAGCCTTAC AATGATATTG GAATCGGTCA GTCTCAAGAT
    GACAGCATTG TCGGTCTCCG ACAGACCAAG CACATGCCGG CTACACTACC TGTCAGTGGG
    ACATTATCTT CCAGCAATCC GGATTTACTG CAGTCCCATC ACCGCATCTT AGACTTTAAT
    ACTACACCAG AACAACCGGA CCAGGTGCTG AGAGTTTTCA AGGCCGATCA GCAGAGCCGT
    TACATAATGA TAAGCAAGGA CACCACCGCT AAGGAAGTGG TCATCCAGGC AATCAGAGAA
    TTTGCATTAA CTGCGGTACC TGATGCCTAC TCATTGTGTG AAGTGTCTGT CACCCCTGAA
    GGTGTCATCA AACAAAGGCG GCTTCCTGAT CAGCTTTCCA AGCTGGCTGA CAGAATACAA
    CTCAGTGGCA GGTATTACCT AAAAAACAAC ATGGAAACTG AAACACTATG CTCAGATGAA
    GATGCCCAAG ATCTTCTAAG GGAAAGTCAG ATCTCTTTGC TACAGCTCAG CACCATTGAA
    GTGGCAACAC AGCTGTCTAT GAGAAATTTT GAGCTATTTA GGAACATTGA GCCCACCGAG
    TACATTGATG ACTTGTTCAA ACTGAAAATG AAAACTGGCT GCACCAACCT CAAAAACTTC
    GAAGAAGTCA TTAACCAAGA GACCTTCTGG GTAGCCTCAG AAATTCTAAG GGAAACTAAC
    CAGCTAAAAC GGATGAAGAT CATCAAGCAT TTTATCAAGA TCGCACTACA TTGTAGAGAG
    TGCAAGAACT TCAACTCCAT GTTTGCTATC ATTAGTGGCT TAAATTTGGC ACCAGTTTCA
    AGACTCCGAA CAACTTGGGA AAAACTCCCA AGCAAATATG AGAAACTCTT TCAAGACCTC
    CAGGACCTCT TTGACCCTTC CAGGAACATG GCTAAATATC GGAATGTGCT GAATAGTCAG
    AACCTTCAGC CTCCCATTAT ACCTTTATTT CCTGTCATTA AGAAAGACCT CACCTTCCTT
    CATGAGGGCA ATGACTCCAA AGTAGACGGA TTAGTTAACT TTGAGAAGCT TAGGATGATT
    GCAAAAGAAA TCCGCCATGT GGGAAGAATG GCTTCAGTAA ATATGGATCC AGCTCTTATG
    TTCCGAACCA GGAAGAAGAA GTGGAGGAGT CTGGGGTCTC TGAGTCAAGG CAGCACCAAT
    GCTACGGTCC TCGACGTCGC CCAGTCGGGG GGACACAAAA AACGTGTGCG TCGCAGCTCT
    TTCCTTAATG CCAAGAAACT GTACGAAGAT GCTCAGATGG CTCGCAAGGT GAAGCAATAC
    TTATCTGATC TAGATCTTGA AATGGATGAA GAGAATCTTC AGACACTTTC TGTACAGTGT
    GAACCAGCAA CCAACACATT GCCAAAAAAT CCATGTGAGA AGAGAGCAGG GAAGCCAGAC
    ACATCGCCTG TGGCACCACG GGCAGCAGCA CAACACAAAC AGCAACAGCA GCAGCAAACT
    AAGGTCAACC AGGCATTGCA AGTTCCTGCT GTGGCTCTTT ACCCATCTCG TAAGAAAGTG
    CCAGTCAAGG ATCTTCCACC CTTCGGCATC AACTCTCCCC AAGCTTTAAA GAAAATTCTT
    TCGTTATCAG AAGAAGGAAG TTTGGAAAGG CACAAGAAGC AAGCAGAGGA CACCATATCA
    AATGCATCCT CGCAGCTTTC ATCCCCTCCG ACATCCCCGC AGTGCTCTCC AAGGAAAGGT
    TACACAATGG CAGCTAGTGG CACAGTGGAT AACTTCTCTG ATTCAGGGCA CAGTGAAATC
    TCCTCCCGCT CCAGCATTGT CAGTAACTCT TCTTTTGACT GCGTGCACTT GCACGATGAC
    CGGCGGCAGA GGCATTCCCT AAGCATAGTG GAGACCAATC TTGGTGTGGG AAGGGCTGAC
    AGAAGAGCCA TTATAGATGC AGACCAGTGC AGCCTAGGCT CTTACGCGCC AGTGGCAGAG
    ACTCGGGGCC TCTATGCAGG TGCAACAGTT CTCTCCTCCC CCAGCATGGA GGAGCTTTCC
    CAGGATCAGG GGGATCGAGC TTCTTTAGAT GCAGCAGATA GTGGCCGTGG AAGCTGGACT
    TCATGTTCCA GCGGATCCCA TGATAACATA CAAACTATCC CACATCAGAG AAGCTGGGAG
    ACTCTTCCTT TTGGTCATGT TCATTTTGAT AACACAGCTG ATGGGTCAGG GTTTTGGGCC
    ACAGGTGGCC ACATGGACCA CGGGATATTT CCTGATCATA GCACAAAATA TGGCAGATAC
    AGCCAAGGAA GAGAACCTTT GGATCAAGCA CAGTCAAGAG CCAGCTGGGC ATCATCAACA
    GGCTACTGGG GCGAGGACTC AGAAGGTGAT ACCGGCACCA TTAAGAGAAG AGGTGGGAAA
    GATGTGGCCC TTGAAGCTGA AACCAGCAGT ATAACATCAA TGACTCCTGA AGAAACAAAG
    CCTCTCCCTG TACCCACACA TGTAGCTGTG ACTGCAAGTT CTGCAAAAGG GCTCATTGTG
    TATTGTGTTA CCACAGCAAG GAAAGAAGGC CGGTACAGAG AACCGCCGCC CACACCTCCA
    GGCTATGTAG GAATCCCCAT AGCTGACTTT GCCGATGGAC ACCCACCTCT ATCCAGAAAG
    CCACCAGATT ACAATGTGGC CTTGCAGCGT TCCAGAATGA TGGCTCGGAG AAGTGAAAGT
    GAAGGGTCAG CAGCAACCTC TCCGCCATCA CATAATCAGA CTTCTGGCAG GACTGCAAAC
    AAACCCCAGT GGCATAAACC AAACGAGGCT GACCCAAGGC TTGCTCTCTA TCAGGCACAA
    GGGGTTACAG AAAAAGATGA TGGTAATTTT TTTGCCGATG ATGAGCAAGT ATCTGCCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811973.1
    CDS325..4827
    Translation

    Target ORF information:

    RefSeq Version XM_012956519.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 2 (rapgef2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAACCTC TTGCATTCCC ATCTAGTCAT GGAGTTATGG GCCAGCAGGA GAAGCATTCT 
    CTCCCTGCTG ATTTCACTAA ACTGCACATC ACAGACAGTC TACATCCACA GGTGACGCAC
    GTGTCCTCTA GTCATTCAGG ATGTAGCATC ACTAGCGATT CTGGGAGCAG CAGCTTGTCT
    GACATCTACC AGGCCACAGA AAGTGAGCCA GGAGATATGG ACCTGAGTGG GCTGCCAGAG
    ACCGCTGTAG ACTCAGAAGA TGACGATGAT GAAGAGGATA TTGAAAGAGC TTCTGACCCT
    TTAATGAGCA GGGACATTGT CAGAGACTGT CTGGAGAAGG ATCCAATTGA CAGGACTGAC
    GACGATATTG AACAACTTCT GGAGTTCATG CACCAGTTGC CAGCATTTGC CAACATGACA
    ATGTCTGTAA GACGAGAGCT CTGTGCAGTC ATGGTGTTCG CTGTGGTCGA GCGGGCAGGA
    ACTGTTGTAC TAAATGATGG GGAGGAGCTC GATTCCTGGT CAGTTATTTT AAATGGCTCA
    GTAGAAGTGA CATATCCTGA TGGCAGGACA GAAATACTTT GCATGGGGAA CAGCTTTGGT
    GTGTCGCCAA CCATGGAGAA GGAATACATG AAGGGAGTCA TGAGAACCAA AGTGGATGAC
    TGCCAGTTTG TGTGCATCGC CCAGCAAGAT TACTGCCGCA TCCTCAATCA GGTGGAAAAG
    AACATGCAGA AAGTGGAAGA GGAGGGTGAG ATTGTGATGG TGAAAGAGCA CAGAGAGCTT
    GATCGCACCG GAACCAGGAA AGGTCATATT GTCATCAAGG GAACATCGGA ACGCCTAACC
    ATGCATCTTG TGGAAGAACA TTCTGTGGTT GACCCAACAT ATATTGAAGA TTTTCTCCTC
    ACTTACAGAA CTTTCCTTTC TAGTCCGATG GAAGTGGGAA AGAAACTACT GGAGTGGTTT
    AATGACCCTA GTCTCAGGGA CAAGGTTACA CGAGTAGTTT TGCTCTGGGT AAATAATCAC
    TTTAATGACT TTGAAGGGGA CCCAGCCATG ACTCGATTTT TGGAAGAATT TGAGAACAAT
    TTGGAACGTG AGAAAATGGG TGGCCACCTA AGGCTATTAA ATATTGCATG TGCTGCCAAA
    GCAAAAAGAA GATTGATAAC TCTAACAAAG CCCTCCCGAG AAGCCCCCTT GCCTTTTAAC
    CTGCTGGGAG GGTCAGACAA GGGATTTGGC ATCTTTGTAG ACAGCGTGGA TCCAGGAAGC
    AAAGCAGCAG AGACTGGCTT AAAGCGAGGG GATCAGATTT TGGAGGTAAA TGGGCAGAAC
    TTTGAAAACA TTCAACTTAC CAAGGCTTTG GAAATCCTAA GGAACAATAC TCACTTGTCT
    CTCACTGTGA AAACAAATTT ATTTGTGTTT AAGGAACTTC TGGCCAGAAT GTTAGAGGAA
    AAGAGAAATA GTGCCCCACA CTTGCCCAAA ATTGGAGACA TCAAAAAAGC CAGCCGCTAT
    TCTATTCCAG ACCTTGCCGT TGATGTTGAA CAAGTTATTG GACTTGAAAA AGTGAGCAAA
    AAGGGCAAAG CCAATACTGT CGGAGGACGA AACAAGCTGA AAAAGATTCT TGACAAGACA
    CGGATCAGTA TCCTGCCTCA AAAGCCTTAC AATGATATTG GAATCGGTCA GTCTCAAGAT
    GACAGCATTG TCGGTCTCCG ACAGACCAAG CACATGCCGG CTACACTACC TGTCAGTGGG
    ACATTATCTT CCAGCAATCC GGATTTACTG CAGTCCCATC ACCGCATCTT AGACTTTAAT
    ACTACACCAG AACAACCGGA CCAGGTGCTG AGAGTTTTCA AGGCCGATCA GCAGAGCCGT
    TACATAATGA TAAGCAAGGA CACCACCGCT AAGGAAGTGG TCATCCAGGC AATCAGAGAA
    TTTGCATTAA CTGCGGTACC TGATGCCTAC TCATTGTGTG AAGTGTCTGT CACCCCTGAA
    GGTGTCATCA AACAAAGGCG GCTTCCTGAT CAGCTTTCCA AGCTGGCTGA CAGAATACAA
    CTCAGTGGCA GGTATTACCT AAAAAACAAC ATGGAAACTG AAACACTATG CTCAGATGAA
    GATGCCCAAG ATCTTCTAAG GGAAAGTCAG ATCTCTTTGC TACAGCTCAG CACCATTGAA
    GTGGCAACAC AGCTGTCTAT GAGAAATTTT GAGCTATTTA GGAACATTGA GCCCACCGAG
    TACATTGATG ACTTGTTCAA ACTGAAAATG AAAACTGGCT GCACCAACCT CAAAAACTTC
    GAAGAAGTCA TTAACCAAGA GACCTTCTGG GTAGCCTCAG AAATTCTAAG GGAAACTAAC
    CAGCTAAAAC GGATGAAGAT CATCAAGCAT TTTATCAAGA TCGCACTACA TTGTAGAGAG
    TGCAAGAACT TCAACTCCAT GTTTGCTATC ATTAGTGGCT TAAATTTGGC ACCAGTTTCA
    AGACTCCGAA CAACTTGGGA AAAACTCCCA AGCAAATATG AGAAACTCTT TCAAGACCTC
    CAGGACCTCT TTGACCCTTC CAGGAACATG GCTAAATATC GGAATGTGCT GAATAGTCAG
    AACCTTCAGC CTCCCATTAT ACCTTTATTT CCTGTCATTA AGAAAGACCT CACCTTCCTT
    CATGAGGGCA ATGACTCCAA AGTAGACGGA TTAGTTAACT TTGAGAAGCT TAGGATGATT
    GCAAAAGAAA TCCGCCATGT GGGAAGAATG GCTTCAGTAA ATATGGATCC AGCTCTTATG
    TTCCGAACCA GGAAGAAGAA GTGGAGGAGT CTGGGGTCTC TGAGTCAAGG CAGCACCAAT
    GCTACGGTCC TCGACGTCGC CCAGTCGGGG GGACACAAAA AACGTGTGCG TCGCAGCTCT
    TTCCTTAATG CCAAGAAACT GTACGAAGAT GCTCAGATGG CTCGCAAGGT GAAGCAATAC
    TTATCTGATC TAGATCTTGA AATGGATGAA GAGAATCTTC AGACACTTTC TGTACAGTGT
    GAACCAGCAA CCAACACATT GCCAAAAAAT CCATGTGAGA AGAGAGCAGG GAAGCCAGAC
    ACATCGCCTG TGGCACCACG GGCAGCAGCA CAACACAAAC AGCAACAGCA GCAGCAAACT
    AAGGTCAACC AGGCATTGCA AGTTCCTGCT GTGGCTCTTT ACCCATCTCG TAAGAAAGTG
    CCAGTCAAGG ATCTTCCACC CTTCGGCATC AACTCTCCCC AAGCTTTAAA GAAAATTCTT
    TCGTTATCAG AAGAAGGAAG TTTGGAAAGG CACAAGAAGC AAGCAGAGGA CACCATATCA
    AATGCATCCT CGCAGCTTTC ATCCCCTCCG ACATCCCCGC AGTGCTCTCC AAGGAAAGGT
    TACACAATGG CAGCTAGTGG CACAGTGGAT AACTTCTCTG ATTCAGGGCA CAGTGAAATC
    TCCTCCCGCT CCAGCATTGT CAGTAACTCT TCTTTTGACT GCGTGCACTT GCACGATGAC
    CGGCGGCAGA GGCATTCCCT AAGCATAGTG GAGACCAATC TTGGTGTGGG AAGGGCTGAC
    AGAAGAGCCA TTATAGATGC AGACCAGTGC AGCCTAGGCT CTTACGCGCC AGTGGCAGAG
    ACTCGGGGCC TCTATGCAGG TGCAACAGTT CTCTCCTCCC CCAGCATGGA GGAGCTTTCC
    CAGGATCAGG GGGATCGAGC TTCTTTAGAT GCAGCAGATA GTGGCCGTGG AAGCTGGACT
    TCATGTTCCA GCGGATCCCA TGATAACATA CAAACTATCC CACATCAGAG AAGCTGGGAG
    ACTCTTCCTT TTGGTCATGT TCATTTTGAT AACACAGCTG ATGGGTCAGG GTTTTGGGCC
    ACAGGTGGCC ACATGGACCA CGGGATATTT CCTGATCATA GCACAAAATA TGGCAGATAC
    AGCCAAGGAA GAGAACCTTT GGATCAAGCA CAGTCAAGAG CCAGCTGGGC ATCATCAACA
    GGCTACTGGG GCGAGGACTC AGAAGGTGAT ACCGGCACCA TTAAGAGAAG AGGTGGGAAA
    GATGTGGCCC TTGAAGCTGA AACCAGCAGT ATAACATCAA TGACTCCTGA AGAAACAAAG
    CCTCTCCCTG TACCCACACA TGTAGCTGTG ACTGCAAGTT CTGCAAAAGG GCTCATTGTG
    TATTGTGTTA CCACAGCAAG GAAAGAAGGC CGGTACAGAG AACCGCCGCC CACACCTCCA
    GGCTATGTAG GAATCCCCAT AGCTGACTTT GCCGATGGAC ACCCACCTCT ATCCAGAAAG
    CCACCAGATT ACAATGTGGC CTTGCAGCGT TCCAGAATGA TGGCTCGGAG AAGTGAAAGT
    GAAGGGTCAG CAGCAACCTC TCCGCCATCA CATAATCAGA CTTCTGGCAG GACTGCAAAC
    AAACCCCAGT GGCATAAACC AAACGAGGCT GACCCAAGGC TTGCTCTCTA TCAGGCACAA
    GGGGTTACAG AAAAAGATGA TGGTAATTTT TTTGCCGATG ATGAGCAAGT ATCTGCCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.