RAPGEF2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014119396.1	XP_013974871.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_003639497.3	XP_003639545.1	rap guanine nucleotide exchange factor 2 isoform X12
XM_022403556.1	XP_022259264.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_014119393.1	XP_013974868.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_014119395.1	XP_013974870.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_014119397.1	XP_013974872.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_014119399.1	XP_013974874.1	rap guanine nucleotide exchange factor 2 isoform X8
XM_014119398.2	XP_013974873.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_005629385.2	XP_005629442.2	rap guanine nucleotide exchange factor 2 isoform X5
XM_014119398.1	XP_013974873.1	rap guanine nucleotide exchange factor 2 isoform X7
XM_014119394.1	XP_013974869.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_005629386.2	XP_005629443.1	rap guanine nucleotide exchange factor 2 isoform X9
XM_005629387.2	XP_005629444.1	rap guanine nucleotide exchange factor 2 isoform X11
XM_022403557.1	XP_022259265.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_022403555.1	XP_022259263.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_022403559.1	XP_022259267.1	rap guanine nucleotide exchange factor 2 isoform X9
XM_003639497.4	XP_003639545.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_014119400.1	XP_013974875.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_022403554.1	XP_022259262.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_022403558.1	XP_022259266.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_014119399.2	XP_013974874.1	rap guanine nucleotide exchange factor 2 isoform X7
XM_005629386.3	XP_005629443.1	rap guanine nucleotide exchange factor 2 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe17765	XM_022403558.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293745	XM_022403554.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe293746	XM_022403555.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293747	XM_022403556.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293748	XM_022403557.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293749	XM_022403559.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe40183	XM_014119393.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40184	XM_014119394.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40185	XM_014119395.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40186	XM_014119396.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe40187	XM_005629385.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe40188	XM_014119397.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe40189	XM_014119398.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe40190	XM_014119399.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe40191	XM_014119400.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe17766	XM_005629386.3 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe17767	XM_005629387.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe17768	XM_003639497.4 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe40189	XM_014119398.1	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe17768	XM_003639497.3	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe40190	XM_014119399.1	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe17766	XM_005629386.2	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

RAPGEF2 ( XM_022403558.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259266.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe17765
Clone ID Related Accession (Same CDS sequence)	XM_022403558.1
Accession Version	XM_022403558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GCTACACCTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTCAGCAATT CTTCCTTTGA CTCCGTGCCA GTCTCGCTGC ATGAGGAGAG GCGGCAGAGG 
CATTCCGTCA GCATCGTGGA AACAAACCTC GGCGTGGGCA GGATGGAAAG GCGGACTATG 
ATGGAACCAG ATCAGTACAG CTTAGGGTCC TATGCACCAA TGGCTGAGAG TCGAGGCTTA 
TATGCTACAG CTACGGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA AGATCAGGGG 
GATCGGGCTT CACTCGATGC TGCCGACAGT GGCCGTGGGA GCTGGACGTC GTGCTCAAGC 
GGTTCACATG ATAACATACA GACCATCCAG CACCAGAGAA GCTGGGAAAC ACTTCCATTT 
GGGCATACTC ACTTTGACTA TTCAGGGGAT CCTGCAGGTT TATGGGCCTC AAGCAGCCAT 
ATGGACCAAA TAATGTTTTC CGATCACAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGTCTTG AACAAGCTCA GTCCCGGGCA AGCTGGGCAT CTTCCACAGG GTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATC AAGCGAAGGG GTGGAAAAGA TGTTTCCATT 
GAAGCTGAAA GCAGTAGCGT TACCTCTGTG ACCACAGAAG AAACCAAACC TGTTCCCATG 
CCTGCCCACG TGGCTGTGAC ATCGAGCACT GCAAAGGGAC TTATTGTGCG GAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCA GGCTACATTG GAATCCCCAT CACTGACTTT 
CCCGAAGGAC ATTCCCATCC AGCCAGGAAG CCTCCAGACT ACAATGTGGC ACTTCAGAGA 
TCTCGGATGG TCGCCCGACC CACTGACACA GCCGCGCCTT CGCCCATACA GCAGCCGCAC 
GGGCATCCGG CGAGCGGAAG GCCGGTGAAC AAACCACAGT GGCATAAACC CAATGAGTGT 
GACCCGCGCC TCGCCCCCTA TCAGTCTCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTATCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259266.1
CDS	124..5001
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403558.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403558.1

ORF Insert Sequence:

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GCTACACCTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTCAGCAATT CTTCCTTTGA CTCCGTGCCA GTCTCGCTGC ATGAGGAGAG GCGGCAGAGG 
CATTCCGTCA GCATCGTGGA AACAAACCTC GGCGTGGGCA GGATGGAAAG GCGGACTATG 
ATGGAACCAG ATCAGTACAG CTTAGGGTCC TATGCACCAA TGGCTGAGAG TCGAGGCTTA 
TATGCTACAG CTACGGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA AGATCAGGGG 
GATCGGGCTT CACTCGATGC TGCCGACAGT GGCCGTGGGA GCTGGACGTC GTGCTCAAGC 
GGTTCACATG ATAACATACA GACCATCCAG CACCAGAGAA GCTGGGAAAC ACTTCCATTT 
GGGCATACTC ACTTTGACTA TTCAGGGGAT CCTGCAGGTT TATGGGCCTC AAGCAGCCAT 
ATGGACCAAA TAATGTTTTC CGATCACAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGTCTTG AACAAGCTCA GTCCCGGGCA AGCTGGGCAT CTTCCACAGG GTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATC AAGCGAAGGG GTGGAAAAGA TGTTTCCATT 
GAAGCTGAAA GCAGTAGCGT TACCTCTGTG ACCACAGAAG AAACCAAACC TGTTCCCATG 
CCTGCCCACG TGGCTGTGAC ATCGAGCACT GCAAAGGGAC TTATTGTGCG GAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCA GGCTACATTG GAATCCCCAT CACTGACTTT 
CCCGAAGGAC ATTCCCATCC AGCCAGGAAG CCTCCAGACT ACAATGTGGC ACTTCAGAGA 
TCTCGGATGG TCGCCCGACC CACTGACACA GCCGCGCCTT CGCCCATACA GCAGCCGCAC 
GGGCATCCGG CGAGCGGAAG GCCGGTGAAC AAACCACAGT GGCATAAACC CAATGAGTGT 
GACCCGCGCC TCGCCCCCTA TCAGTCTCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTATCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_022403554.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259262.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe293745
Clone ID Related Accession (Same CDS sequence)	XM_022403554.1
Accession Version	XM_022403554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GTGGCAGTGG CAATCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGACTTA ACCAGTTCCT CCTCTTCTCT TGGAAGTGAG 
AACAGTAACA AGAATAACAA TGCACCACGG ACCTATGGGA TAGGCTACAC CTTGGCTCCC 
AGTGGTACTG TGGATAATTT TTCAGATTCT GGTCACAGTG AAATTTCTTC ACGATCCAGT 
ATTGTCAGCA ATTCTTCCTT TGACTCCGTG CCAGTCTCGC TGCATGAGGA GAGGCGGCAG 
AGGCATTCCG TCAGCATCGT GGAAACAAAC CTCGGCGTGG GCAGGATGGA AAGGCGGACT 
ATGATGGAAC CAGATCAGTA CAGCTTAGGG TCCTATGCAC CAATGGCTGA GAGTCGAGGC 
TTATATGCTA CAGCTACGGT AATTTCTTCT CCAAGCACAG AGGAACTTTC CCAAGATCAG 
GGGGATCGGG CTTCACTCGA TGCTGCCGAC AGTGGCCGTG GGAGCTGGAC GTCGTGCTCA 
AGCGGTTCAC ATGATAACAT ACAGACCATC CAGCACCAGA GAAGCTGGGA AACACTTCCA 
TTTGGGCATA CTCACTTTGA CTATTCAGGG GATCCTGCAG GTTTATGGGC CTCAAGCAGC 
CATATGGACC AAATAATGTT TTCCGATCAC AGCACAAAGT ATAACAGGCA AAATCAAAGT 
AGAGAGAGTC TTGAACAAGC TCAGTCCCGG GCAAGCTGGG CATCTTCCAC AGGGTACTGG 
GGAGAAGACT CAGAAGGTGA CACAGGCACA ATCAAGCGAA GGGGTGGAAA AGATGTTTCC 
ATTGAAGCTG AAAGCAGTAG CGTTACCTCT GTGACCACAG AAGAAACCAA ACCTGTTCCC 
ATGCCTGCCC ACGTGGCTGT GACATCGAGC ACTGCAAAGG GACTTATTGT GCGGAAGGAG 
GGCAGGTATC GAGAGCCCCC GCCCACCCCT CCAGGCTACA TTGGAATCCC CATCACTGAC 
TTTCCCGAAG GACATTCCCA TCCAGCCAGG AAGCCTCCAG ACTACAATGT GGCACTTCAG 
AGATCTCGGA TGGTCGCCCG ACCCACTGAC ACAGCCGCGC CTTCGCCCAT ACAGCAGCCG 
CACGGGCATC CGGCGAGCGG AAGGCCGGTG AACAAACCAC AGTGGCATAA ACCCAATGAG 
TGTGACCCGC GCCTCGCCCC CTATCAGTCT CAAGGGTTTT CCACCGAGGA GGATGAAGAT 
GAACAAGTAT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259262.1
CDS	124..5124
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403554.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403554.1

ORF Insert Sequence:

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GTGGCAGTGG CAATCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGACTTA ACCAGTTCCT CCTCTTCTCT TGGAAGTGAG 
AACAGTAACA AGAATAACAA TGCACCACGG ACCTATGGGA TAGGCTACAC CTTGGCTCCC 
AGTGGTACTG TGGATAATTT TTCAGATTCT GGTCACAGTG AAATTTCTTC ACGATCCAGT 
ATTGTCAGCA ATTCTTCCTT TGACTCCGTG CCAGTCTCGC TGCATGAGGA GAGGCGGCAG 
AGGCATTCCG TCAGCATCGT GGAAACAAAC CTCGGCGTGG GCAGGATGGA AAGGCGGACT 
ATGATGGAAC CAGATCAGTA CAGCTTAGGG TCCTATGCAC CAATGGCTGA GAGTCGAGGC 
TTATATGCTA CAGCTACGGT AATTTCTTCT CCAAGCACAG AGGAACTTTC CCAAGATCAG 
GGGGATCGGG CTTCACTCGA TGCTGCCGAC AGTGGCCGTG GGAGCTGGAC GTCGTGCTCA 
AGCGGTTCAC ATGATAACAT ACAGACCATC CAGCACCAGA GAAGCTGGGA AACACTTCCA 
TTTGGGCATA CTCACTTTGA CTATTCAGGG GATCCTGCAG GTTTATGGGC CTCAAGCAGC 
CATATGGACC AAATAATGTT TTCCGATCAC AGCACAAAGT ATAACAGGCA AAATCAAAGT 
AGAGAGAGTC TTGAACAAGC TCAGTCCCGG GCAAGCTGGG CATCTTCCAC AGGGTACTGG 
GGAGAAGACT CAGAAGGTGA CACAGGCACA ATCAAGCGAA GGGGTGGAAA AGATGTTTCC 
ATTGAAGCTG AAAGCAGTAG CGTTACCTCT GTGACCACAG AAGAAACCAA ACCTGTTCCC 
ATGCCTGCCC ACGTGGCTGT GACATCGAGC ACTGCAAAGG GACTTATTGT GCGGAAGGAG 
GGCAGGTATC GAGAGCCCCC GCCCACCCCT CCAGGCTACA TTGGAATCCC CATCACTGAC 
TTTCCCGAAG GACATTCCCA TCCAGCCAGG AAGCCTCCAG ACTACAATGT GGCACTTCAG 
AGATCTCGGA TGGTCGCCCG ACCCACTGAC ACAGCCGCGC CTTCGCCCAT ACAGCAGCCG 
CACGGGCATC CGGCGAGCGG AAGGCCGGTG AACAAACCAC AGTGGCATAA ACCCAATGAG 
TGTGACCCGC GCCTCGCCCC CTATCAGTCT CAAGGGTTTT CCACCGAGGA GGATGAAGAT 
GAACAAGTAT CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_022403555.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259263.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe293746
Clone ID Related Accession (Same CDS sequence)	XM_022403555.1
Accession Version	XM_022403555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGCTTCCTG CAGATTTCAC AAAACTACAC CTTACTGATA GTCTCCACCC ACAGGTGACG 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGTGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCTGA GGACGATGAT GATGAAGAAG ACATTGAGAG GGCATCAGAT 
CCTCTGATGA GTAGAGATAT TGTGAGAGAC TGTCTAGAGA AGGATCCAAT TGACAGGACA 
GATGATGACA TTGAACAACT CTTAGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGACGAGA ACTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGATTCCT GGTCAGTGAT TCTGAATGGT 
TCTGTGGAAG TAACTTATCC AGATGGAAAA GCAGAAATAT TATGTATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TAATGAGAAC AAAAGTGGAT 
GACTGCCAGT TTGTCTGTAT AGCCCAGCAA GATTACTGCC GTATTCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA GGAAGAAGGA GAAATTGTTA TGGTAAAAGA ACACCGAGAA 
CTTGATAGAA CTGGAACAAG AAAGGGACAT ATTGTCATCA AGGGCACCTC AGAAAGATTA 
ACAATGCATT TGGTGGAAGA GCATTCGGTG GTGGATCCAA CATTCATAGA GGACTTCCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AACCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCTC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GTGGCTCAGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGA GGGGACCAGA TATTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTAGGAATAA CACACACTTA 
TCAATCACTG TGAAAACCAA CTTATTCGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTAAAT 
AAAAAAAGTA AAGCCAATAC TGTTGGAGGA AGGAACAAGC TAAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGTA TAGTGGGATT AAGACAGACG AAGCACATCC CAACTGCACT GCCCGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATTTA TTACAGTCAC ATCATCGCAT TCTGGACTTC 
AGTACTACTC CTGACTTGCC AGATCAAGTG TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACCACA GCGAAGGAAG TAGTCATTCA GGCCATCAGG 
GAGTTTGCTG TGACTGCCAC CCCGGATCAA TATTCGCTGT GCGAGGTCTC GGTCACACCT 
GAGGGTGTCA TCAAACAAAG ACGGCTTCCA GATCAGCTTT CCAAACTTGC TGATAGAATA 
CAATTGAGTG GAAGGTACTA CTTGAAAAAC AACATGGAGA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGCTGCT GAGGGAGAGT CAAATCTCCC TGTTGCAGCT CAGTACTGTT 
GAAGTTGCCA CACAGCTCTC TATGCGAAAC TTTGAACTCT TCCGTAACAT TGAACCCACC 
GAATATATAG ATGATTTATT TAAACTCAAA TCTAAAACCA GCTGTGCCAA CCTGAAGAAA 
TTTGAAGAAG TAATTAACCA AGAAACATTT TGGGTAGCAT CTGAAATTCT GAGAGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCACT GCACTGCAGG 
GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACTT GGCCCCAGTG 
GCAAGACTAC GGACCACCTG GGAAAAACTT CCCAATAAAT ATGAAAAGCT GTTTCAAGAT 
CTCCAGGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGCAATGT CCTCAATAGT 
CAAAACCTGC AGCCTCCCAT AATCCCCCTA TTCCCAGTTA TCAAGAAGGA TCTCACATTC 
CTTCATGAAG GAAATGACTC AAAAGTAGAT GGGTTAGTCA ATTTTGAGAA GCTAAGGATG 
ATCGCAAAAG AAATTCGTCA TGTTGGCCGA ATGGCTTCAG TTAACATGGA CCCTGCCCTC 
ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGACGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTGTCCA ATTTGGAGCT AGAAATGGAT GAGGAGAGTC TTCAGACATT ATCACTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAGTCT 
GAGACGTCCC CAGTGGCTCC AAGGGCAGGG TCACAACAGA AAGCACAAGC CCAGCCGCCG 
CCCCCGCAGC CACAGCCCCA ACATAAGATC AACCAAGGCC TTCAGGTTCC TGCTGTATCC 
CTTTATCCTT CACGAAAGAA AGTACCTGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAGAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTCTGGA GCGTCATAAA 
AAACAAGCAG AAGATACTAT ATCAAATGCA TCCTCACAGC TTTCTTCTCC TCCCACTTCT 
CCACAAAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAC ACCTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTCAG CAATTCTTCC 
TTTGACTCCG TGCCAGTCTC GCTGCATGAG GAGAGGCGGC AGAGGCATTC CGTCAGCATC 
GTGGAAACAA ACCTCGGCGT GGGCAGGATG GAAAGGCGGA CTATGATGGA ACCAGATCAG 
TACAGCTTAG GGTCCTATGC ACCAATGGCT GAGAGTCGAG GCTTATATGC TACAGCTACG 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAAGATC AGGGGGATCG GGCTTCACTC 
GATGCTGCCG ACAGTGGCCG TGGGAGCTGG ACGTCGTGCT CAAGCGGTTC ACATGATAAC 
ATACAGACCA TCCAGCACCA GAGAAGCTGG GAAACACTTC CATTTGGGCA TACTCACTTT 
GACTATTCAG GGGATCCTGC AGGTTTATGG GCCTCAAGCA GCCATATGGA CCAAATAATG 
TTTTCCGATC ACAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG TCTTGAACAA 
GCTCAGTCCC GGGCAAGCTG GGCATCTTCC ACAGGGTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATCAAGCG AAGGGGTGGA AAAGATGTTT CCATTGAAGC TGAAAGCAGT 
AGCGTTACCT CTGTGACCAC AGAAGAAACC AAACCTGTTC CCATGCCTGC CCACGTGGCT 
GTGACATCGA GCACTGCAAA GGGACTTATT GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCAGGCTA CATTGGAATC CCCATCACTG ACTTTCCCGA AGGACATTCC 
CATCCAGCCA GGAAGCCTCC AGACTACAAT GTGGCACTTC AGAGATCTCG GATGGTCGCC 
CGACCCACTG ACACAGCCGC GCCTTCGCCC ATACAGCAGC CGCACGGGCA TCCGGCGAGC 
GGAAGGCCGG TGAACAAACC ACAGTGGCAT AAACCCAATG AGTGTGACCC GCGCCTCGCC 
CCCTATCAGT CTCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT ATCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259263.1
CDS	124..5106
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403555.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403555.1

ORF Insert Sequence:

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGCTTCCTG CAGATTTCAC AAAACTACAC CTTACTGATA GTCTCCACCC ACAGGTGACG 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGTGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCTGA GGACGATGAT GATGAAGAAG ACATTGAGAG GGCATCAGAT 
CCTCTGATGA GTAGAGATAT TGTGAGAGAC TGTCTAGAGA AGGATCCAAT TGACAGGACA 
GATGATGACA TTGAACAACT CTTAGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGACGAGA ACTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGATTCCT GGTCAGTGAT TCTGAATGGT 
TCTGTGGAAG TAACTTATCC AGATGGAAAA GCAGAAATAT TATGTATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TAATGAGAAC AAAAGTGGAT 
GACTGCCAGT TTGTCTGTAT AGCCCAGCAA GATTACTGCC GTATTCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA GGAAGAAGGA GAAATTGTTA TGGTAAAAGA ACACCGAGAA 
CTTGATAGAA CTGGAACAAG AAAGGGACAT ATTGTCATCA AGGGCACCTC AGAAAGATTA 
ACAATGCATT TGGTGGAAGA GCATTCGGTG GTGGATCCAA CATTCATAGA GGACTTCCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AACCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCTC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GTGGCTCAGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGA GGGGACCAGA TATTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTAGGAATAA CACACACTTA 
TCAATCACTG TGAAAACCAA CTTATTCGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTAAAT 
AAAAAAAGTA AAGCCAATAC TGTTGGAGGA AGGAACAAGC TAAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGTA TAGTGGGATT AAGACAGACG AAGCACATCC CAACTGCACT GCCCGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATTTA TTACAGTCAC ATCATCGCAT TCTGGACTTC 
AGTACTACTC CTGACTTGCC AGATCAAGTG TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACCACA GCGAAGGAAG TAGTCATTCA GGCCATCAGG 
GAGTTTGCTG TGACTGCCAC CCCGGATCAA TATTCGCTGT GCGAGGTCTC GGTCACACCT 
GAGGGTGTCA TCAAACAAAG ACGGCTTCCA GATCAGCTTT CCAAACTTGC TGATAGAATA 
CAATTGAGTG GAAGGTACTA CTTGAAAAAC AACATGGAGA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGCTGCT GAGGGAGAGT CAAATCTCCC TGTTGCAGCT CAGTACTGTT 
GAAGTTGCCA CACAGCTCTC TATGCGAAAC TTTGAACTCT TCCGTAACAT TGAACCCACC 
GAATATATAG ATGATTTATT TAAACTCAAA TCTAAAACCA GCTGTGCCAA CCTGAAGAAA 
TTTGAAGAAG TAATTAACCA AGAAACATTT TGGGTAGCAT CTGAAATTCT GAGAGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCACT GCACTGCAGG 
GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACTT GGCCCCAGTG 
GCAAGACTAC GGACCACCTG GGAAAAACTT CCCAATAAAT ATGAAAAGCT GTTTCAAGAT 
CTCCAGGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGCAATGT CCTCAATAGT 
CAAAACCTGC AGCCTCCCAT AATCCCCCTA TTCCCAGTTA TCAAGAAGGA TCTCACATTC 
CTTCATGAAG GAAATGACTC AAAAGTAGAT GGGTTAGTCA ATTTTGAGAA GCTAAGGATG 
ATCGCAAAAG AAATTCGTCA TGTTGGCCGA ATGGCTTCAG TTAACATGGA CCCTGCCCTC 
ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGACGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTGTCCA ATTTGGAGCT AGAAATGGAT GAGGAGAGTC TTCAGACATT ATCACTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAGTCT 
GAGACGTCCC CAGTGGCTCC AAGGGCAGGG TCACAACAGA AAGCACAAGC CCAGCCGCCG 
CCCCCGCAGC CACAGCCCCA ACATAAGATC AACCAAGGCC TTCAGGTTCC TGCTGTATCC 
CTTTATCCTT CACGAAAGAA AGTACCTGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAGAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTCTGGA GCGTCATAAA 
AAACAAGCAG AAGATACTAT ATCAAATGCA TCCTCACAGC TTTCTTCTCC TCCCACTTCT 
CCACAAAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAC ACCTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTCAG CAATTCTTCC 
TTTGACTCCG TGCCAGTCTC GCTGCATGAG GAGAGGCGGC AGAGGCATTC CGTCAGCATC 
GTGGAAACAA ACCTCGGCGT GGGCAGGATG GAAAGGCGGA CTATGATGGA ACCAGATCAG 
TACAGCTTAG GGTCCTATGC ACCAATGGCT GAGAGTCGAG GCTTATATGC TACAGCTACG 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAAGATC AGGGGGATCG GGCTTCACTC 
GATGCTGCCG ACAGTGGCCG TGGGAGCTGG ACGTCGTGCT CAAGCGGTTC ACATGATAAC 
ATACAGACCA TCCAGCACCA GAGAAGCTGG GAAACACTTC CATTTGGGCA TACTCACTTT 
GACTATTCAG GGGATCCTGC AGGTTTATGG GCCTCAAGCA GCCATATGGA CCAAATAATG 
TTTTCCGATC ACAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG TCTTGAACAA 
GCTCAGTCCC GGGCAAGCTG GGCATCTTCC ACAGGGTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATCAAGCG AAGGGGTGGA AAAGATGTTT CCATTGAAGC TGAAAGCAGT 
AGCGTTACCT CTGTGACCAC AGAAGAAACC AAACCTGTTC CCATGCCTGC CCACGTGGCT 
GTGACATCGA GCACTGCAAA GGGACTTATT GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCAGGCTA CATTGGAATC CCCATCACTG ACTTTCCCGA AGGACATTCC 
CATCCAGCCA GGAAGCCTCC AGACTACAAT GTGGCACTTC AGAGATCTCG GATGGTCGCC 
CGACCCACTG ACACAGCCGC GCCTTCGCCC ATACAGCAGC CGCACGGGCA TCCGGCGAGC 
GGAAGGCCGG TGAACAAACC ACAGTGGCAT AAACCCAATG AGTGTGACCC GCGCCTCGCC 
CCCTATCAGT CTCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT ATCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_022403556.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259264.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OCe293747
Clone ID Related Accession (Same CDS sequence)	XM_022403556.1
Accession Version	XM_022403556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGTGAGAACA GTAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259264.1
CDS	124..5100
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403556.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403556.1

ORF Insert Sequence:

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGTGAGAACA GTAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_022403557.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259265.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OCe293748
Clone ID Related Accession (Same CDS sequence)	XM_022403557.1
Accession Version	XM_022403557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GTGGCAGTGG CAATCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGACTTA ACCAGTTCCT CCTCTTCTCT TGGAAGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259265.1
CDS	124..5076
Translation	MEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403557.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403557.1

ORF Insert Sequence:

ATGGAAGCCT TATCAAACTT GAGAGAGCAC CAGCTTAGGT TAATGTGTGA AACTGTGAGA 
TATGAAAGAC ATGAAGCAAA TGAAGTTTTA TACTATCCTG ATGATATTGG GACCTGCTGG 
TATATCCTTC TCTCTGGTTC CGTGTTCATT AAGGAATCCA TGTTTCTTCC AAGAAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTTAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCT 
TCTGAAATGA TTGTGGTGGA CTATATGGAT GAAAATGAAG AATATTTTCA GCGTCAAGCT 
TCTCATCGAC AGTCTCGAAG GAGATTTAGA AAAATCAACC AGAAAGGTGA AAGACAAACA 
ATTATTGACA CTGTGGATCC TTATCCTGTG GGCAAACCAC CTTTGCCTCG AGGCTATCAT 
ACGGAATGCA CTAAATCTCA GCTTCCTGCA GATTTCACAA AACTACACCT TACTGATAGT 
CTCCACCCAC AGGTGACGCA CGTTTCTTCT AGCCATTCAG GATGTAGTAT CACTAGTGAT 
TCTGGAAGCA GCAGTCTTTC TGATATCTAC CAGGCCACAG AAAGTGAGGC TGGTGATATG 
GACCTGAGTG GGTTGCCAGA AACAGCAGTG GATTCTGAGG ACGATGATGA TGAAGAAGAC 
ATTGAGAGGG CATCAGATCC TCTGATGAGT AGAGATATTG TGAGAGACTG TCTAGAGAAG 
GATCCAATTG ACAGGACAGA TGATGACATT GAACAACTCT TAGAATTTAT GCACCAGTTG 
CCTGCTTTTG CCAATATGAC AATGTCAGTG AGACGAGAAC TCTGTGCCGT GATGGTGTTT 
GCAGTGGTGG AAAGAGCTGG GACCATAGTG TTAAATGATG GTGAAGAGCT GGATTCCTGG 
TCAGTGATTC TGAATGGTTC TGTGGAAGTA ACTTATCCAG ATGGAAAAGC AGAAATATTA 
TGTATGGGAA ATAGTTTTGG TGTCTCTCCT ACCATGGACA AAGAATACAT GAAAGGAGTA 
ATGAGAACAA AAGTGGATGA CTGCCAGTTT GTCTGTATAG CCCAGCAAGA TTACTGCCGT 
ATTCTCAACC AAGTAGAAAA GAACATGCAA AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAAGAAC ACCGAGAACT TGATAGAACT GGAACAAGAA AGGGACATAT TGTCATCAAG 
GGCACCTCAG AAAGATTAAC AATGCATTTG GTGGAAGAGC ATTCGGTGGT GGATCCAACA 
TTCATAGAGG ACTTCCTGTT GACCTATAGG ACTTTTCTTT CTAGCCCAAT GGAAGTGGGC 
AAAAAGTTAT TGGAGTGGTT TAATGACCCG AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTATTGTGGG TGAATAATCA CTTCAATGAC TTTGAAGGAG ATCCTGCAAT GACTCGATTT 
CTAGAAGAAT TTGAAAATAA CCTGGAAAGA GAGAAAATGG GTGGACATCT AAGGCTGTTA 
AATATTGCAT GTGCAGCTAA AGCAAAAAGA AGATTGATGA CGTTAACAAA ACCATCTCGA 
GAAGCTCCTT TGCCTTTTAT CTTACTTGGT GGCTCAGAGA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTCAGGTAG CAAAGCAACT GAAGCAGGCT TGAAACGAGG GGACCAGATA 
TTAGAAGTAA ATGGTCAAAA CTTTGAAAAT ATTCAGCTGT CAAAAGCCAT GGAAATTCTT 
AGGAATAACA CACACTTATC AATCACTGTG AAAACCAACT TATTCGTATT TAAAGAACTT 
CTAACAAGAT TGTCAGAAGA GAAAAGAAAT GGTGCCCCTC ACCTCCCTAA AATTGGAGAC 
ATCAAAAAGG CCAGTCGCTA CTCCATTCCA GATCTTGCTG TAGATGTAGA ACAGGTGATA 
GGACTTGAAA AAGTAAATAA AAAAAGTAAA GCCAATACTG TTGGAGGAAG GAACAAGCTA 
AAAAAGATAC TCGACAAGAC TCGGATCAGT ATCTTGCCAC AGAAACCATA TAATGATATT 
GGGATTGGTC AGTCTCAAGA TGACAGTATA GTGGGATTAA GACAGACGAA GCACATCCCA 
ACTGCACTGC CCGTCAGTGG AACCTTGTCA TCCAGTAATC CTGATTTATT ACAGTCACAT 
CATCGCATTC TGGACTTCAG TACTACTCCT GACTTGCCAG ATCAAGTGTT AAGGGTTTTT 
AAGGCTGATC AGCAAAGCCG ATACATCATG ATCAGTAAGG ACACCACAGC GAAGGAAGTA 
GTCATTCAGG CCATCAGGGA GTTTGCTGTG ACTGCCACCC CGGATCAATA TTCGCTGTGC 
GAGGTCTCGG TCACACCTGA GGGTGTCATC AAACAAAGAC GGCTTCCAGA TCAGCTTTCC 
AAACTTGCTG ATAGAATACA ATTGAGTGGA AGGTACTACT TGAAAAACAA CATGGAGACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAG GAGCTGCTGA GGGAGAGTCA AATCTCCCTG 
TTGCAGCTCA GTACTGTTGA AGTTGCCACA CAGCTCTCTA TGCGAAACTT TGAACTCTTC 
CGTAACATTG AACCCACCGA ATATATAGAT GATTTATTTA AACTCAAATC TAAAACCAGC 
TGTGCCAACC TGAAGAAATT TGAAGAAGTA ATTAACCAAG AAACATTTTG GGTAGCATCT 
GAAATTCTGA GAGAGACAAA CCAGCTGAAG AGGATGAAGA TCATTAAGCA TTTCATCAAG 
ATTGCACTGC ACTGCAGGGA ATGCAAGAAT TTTAACTCCA TGTTTGCAAT CATCAGTGGC 
CTAAACTTGG CCCCAGTGGC AAGACTACGG ACCACCTGGG AAAAACTTCC CAATAAATAT 
GAAAAGCTGT TTCAAGATCT CCAGGACCTG TTTGATCCTT CCAGAAACAT GGCAAAATAT 
CGCAATGTCC TCAATAGTCA AAACCTGCAG CCTCCCATAA TCCCCCTATT CCCAGTTATC 
AAGAAGGATC TCACATTCCT TCATGAAGGA AATGACTCAA AAGTAGATGG GTTAGTCAAT 
TTTGAGAAGC TAAGGATGAT CGCAAAAGAA ATTCGTCATG TTGGCCGAAT GGCTTCAGTT 
AACATGGACC CTGCCCTCAT GTTCAGAACT CGGAAGAAGA AGTGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GTAGTACAAA TGCAACAGTG CTAGACGTTG CTCAGACAGG TGGTCATAAA 
AAGCGGGTAC GTCGTAGTTC CTTTCTCAAT GCCAAAAAGC TTTATGAAGA TGCCCAAATG 
GCTCGAAAAG TGAAGCAGTA CCTGTCCAAT TTGGAGCTAG AAATGGATGA GGAGAGTCTT 
CAGACATTAT CACTGCAGTG TGAGCCAGCA ACCAACACAT TGCCTAAGAA TCCTGGTGAC 
AAAAAGCCTG TCAAGTCTGA GACGTCCCCA GTGGCTCCAA GGGCAGGGTC ACAACAGAAA 
GCACAAGCCC AGCCGCCGCC CCCGCAGCCA CAGCCCCAAC ATAAGATCAA CCAAGGCCTT 
CAGGTTCCTG CTGTATCCCT TTATCCTTCA CGAAAGAAAG TACCTGTAAA GGATCTCCCA 
CCTTTTGGCA TAAACTCTCC ACAAGCTTTA AAGAAAATTC TTTCTTTGTC TGAAGAAGGA 
AGTCTGGAGC GTCATAAAAA ACAAGCAGAA GATACTATAT CAAATGCATC CTCACAGCTT 
TCTTCTCCTC CCACTTCTCC ACAAAGTTCT CCAAGGAAAG GTGGCAGTGG CAATCAGCTG 
AGATCTTTTG GCTCCGGGCA GTTGGACTTA ACCAGTTCCT CCTCTTCTCT TGGAAGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_022403559.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259267.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

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CloneID	OCe293749
Clone ID Related Accession (Same CDS sequence)	XM_022403559.1
Accession Version	XM_022403559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGCTACA CCTTGGCTCC CAGTGGTACT 
GTGGATAATT TTTCAGATTC TGGTCACAGT GAAATTTCTT CACGATCCAG TATTGTCAGC 
AATTCTTCCT TTGACTCCGT GCCAGTCTCG CTGCATGAGG AGAGGCGGCA GAGGCATTCC 
GTCAGCATCG TGGAAACAAA CCTCGGCGTG GGCAGGATGG AAAGGCGGAC TATGATGGAA 
CCAGATCAGT ACAGCTTAGG GTCCTATGCA CCAATGGCTG AGAGTCGAGG CTTATATGCT 
ACAGCTACGG TAATTTCTTC TCCAAGCACA GAGGAACTTT CCCAAGATCA GGGGGATCGG 
GCTTCACTCG ATGCTGCCGA CAGTGGCCGT GGGAGCTGGA CGTCGTGCTC AAGCGGTTCA 
CATGATAACA TACAGACCAT CCAGCACCAG AGAAGCTGGG AAACACTTCC ATTTGGGCAT 
ACTCACTTTG ACTATTCAGG GGATCCTGCA GGTTTATGGG CCTCAAGCAG CCATATGGAC 
CAAATAATGT TTTCCGATCA CAGCACAAAG TATAACAGGC AAAATCAAAG TAGAGAGAGT 
CTTGAACAAG CTCAGTCCCG GGCAAGCTGG GCATCTTCCA CAGGGTACTG GGGAGAAGAC 
TCAGAAGGTG ACACAGGCAC AATCAAGCGA AGGGGTGGAA AAGATGTTTC CATTGAAGCT 
GAAAGCAGTA GCGTTACCTC TGTGACCACA GAAGAAACCA AACCTGTTCC CATGCCTGCC 
CACGTGGCTG TGACATCGAG CACTGCAAAG GGACTTATTG TGCGGAAGGA GGGCAGGTAT 
CGAGAGCCCC CGCCCACCCC TCCAGGCTAC ATTGGAATCC CCATCACTGA CTTTCCCGAA 
GGACATTCCC ATCCAGCCAG GAAGCCTCCA GACTACAATG TGGCACTTCA GAGATCTCGG 
ATGGTCGCCC GACCCACTGA CACAGCCGCG CCTTCGCCCA TACAGCAGCC GCACGGGCAT 
CCGGCGAGCG GAAGGCCGGT GAACAAACCA CAGTGGCATA AACCCAATGA GTGTGACCCG 
CGCCTCGCCC CCTATCAGTC TCAAGGGTTT TCCACCGAGG AGGATGAAGA TGAACAAGTA 
TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259267.1
CDS	1..4572
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_022403559.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403559.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGCTACA CCTTGGCTCC CAGTGGTACT 
GTGGATAATT TTTCAGATTC TGGTCACAGT GAAATTTCTT CACGATCCAG TATTGTCAGC 
AATTCTTCCT TTGACTCCGT GCCAGTCTCG CTGCATGAGG AGAGGCGGCA GAGGCATTCC 
GTCAGCATCG TGGAAACAAA CCTCGGCGTG GGCAGGATGG AAAGGCGGAC TATGATGGAA 
CCAGATCAGT ACAGCTTAGG GTCCTATGCA CCAATGGCTG AGAGTCGAGG CTTATATGCT 
ACAGCTACGG TAATTTCTTC TCCAAGCACA GAGGAACTTT CCCAAGATCA GGGGGATCGG 
GCTTCACTCG ATGCTGCCGA CAGTGGCCGT GGGAGCTGGA CGTCGTGCTC AAGCGGTTCA 
CATGATAACA TACAGACCAT CCAGCACCAG AGAAGCTGGG AAACACTTCC ATTTGGGCAT 
ACTCACTTTG ACTATTCAGG GGATCCTGCA GGTTTATGGG CCTCAAGCAG CCATATGGAC 
CAAATAATGT TTTCCGATCA CAGCACAAAG TATAACAGGC AAAATCAAAG TAGAGAGAGT 
CTTGAACAAG CTCAGTCCCG GGCAAGCTGG GCATCTTCCA CAGGGTACTG GGGAGAAGAC 
TCAGAAGGTG ACACAGGCAC AATCAAGCGA AGGGGTGGAA AAGATGTTTC CATTGAAGCT 
GAAAGCAGTA GCGTTACCTC TGTGACCACA GAAGAAACCA AACCTGTTCC CATGCCTGCC 
CACGTGGCTG TGACATCGAG CACTGCAAAG GGACTTATTG TGCGGAAGGA GGGCAGGTAT 
CGAGAGCCCC CGCCCACCCC TCCAGGCTAC ATTGGAATCC CCATCACTGA CTTTCCCGAA 
GGACATTCCC ATCCAGCCAG GAAGCCTCCA GACTACAATG TGGCACTTCA GAGATCTCGG 
ATGGTCGCCC GACCCACTGA CACAGCCGCG CCTTCGCCCA TACAGCAGCC GCACGGGCAT 
CCGGCGAGCG GAAGGCCGGT GAACAAACCA CAGTGGCATA AACCCAATGA GTGTGACCCG 
CGCCTCGCCC CCTATCAGTC TCAAGGGTTT TCCACCGAGG AGGATGAAGA TGAACAAGTA 
TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119393.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974868.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OCe40183
Clone ID Related Accession (Same CDS sequence)	XM_014119393.1
Accession Version	XM_014119393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGTGAGAACA GTAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974868.1
CDS	16..5112
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119393.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119393.1

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGTGAGAACA GTAACAAGAA TAACAATGCA 
CCACGGACCT ATGGGATAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119394.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974869.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe40184
Clone ID Related Accession (Same CDS sequence)	XM_014119394.1
Accession Version	XM_014119394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGC TTCCTGCAGA TTTCACAAAA 
CTACACCTTA CTGATAGTCT CCACCCACAG GTGACGCACG TTTCTTCTAG CCATTCAGGA 
TGTAGTATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAA 
AGTGAGGCTG GTGATATGGA CCTGAGTGGG TTGCCAGAAA CAGCAGTGGA TTCTGAGGAC 
GATGATGATG AAGAAGACAT TGAGAGGGCA TCAGATCCTC TGATGAGTAG AGATATTGTG 
AGAGACTGTC TAGAGAAGGA TCCAATTGAC AGGACAGATG ATGACATTGA ACAACTCTTA 
GAATTTATGC ACCAGTTGCC TGCTTTTGCC AATATGACAA TGTCAGTGAG ACGAGAACTC 
TGTGCCGTGA TGGTGTTTGC AGTGGTGGAA AGAGCTGGGA CCATAGTGTT AAATGATGGT 
GAAGAGCTGG ATTCCTGGTC AGTGATTCTG AATGGTTCTG TGGAAGTAAC TTATCCAGAT 
GGAAAAGCAG AAATATTATG TATGGGAAAT AGTTTTGGTG TCTCTCCTAC CATGGACAAA 
GAATACATGA AAGGAGTAAT GAGAACAAAA GTGGATGACT GCCAGTTTGT CTGTATAGCC 
CAGCAAGATT ACTGCCGTAT TCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAGGAA 
GAAGGAGAAA TTGTTATGGT AAAAGAACAC CGAGAACTTG ATAGAACTGG AACAAGAAAG 
GGACATATTG TCATCAAGGG CACCTCAGAA AGATTAACAA TGCATTTGGT GGAAGAGCAT 
TCGGTGGTGG ATCCAACATT CATAGAGGAC TTCCTGTTGA CCTATAGGAC TTTTCTTTCT 
AGCCCAATGG AAGTGGGCAA AAAGTTATTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACAC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAACC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ATTGATGACG 
TTAACAAAAC CATCTCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGTGG CTCAGAGAAG 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGGTAGCA AAGCAACTGA AGCAGGCTTG 
AAACGAGGGG ACCAGATATT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTGTCA 
AAAGCCATGG AAATTCTTAG GAATAACACA CACTTATCAA TCACTGTGAA AACCAACTTA 
TTCGTATTTA AAGAACTTCT AACAAGATTG TCAGAAGAGA AAAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTTGCTGTA 
GATGTAGAAC AGGTGATAGG ACTTGAAAAA GTAAATAAAA AAAGTAAAGC CAATACTGTT 
GGAGGAAGGA ACAAGCTAAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATTGG GATTGGTCAG TCTCAAGATG ACAGTATAGT GGGATTAAGA 
CAGACGAAGC ACATCCCAAC TGCACTGCCC GTCAGTGGAA CCTTGTCATC CAGTAATCCT 
GATTTATTAC AGTCACATCA TCGCATTCTG GACTTCAGTA CTACTCCTGA CTTGCCAGAT 
CAAGTGTTAA GGGTTTTTAA GGCTGATCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACCACAGCGA AGGAAGTAGT CATTCAGGCC ATCAGGGAGT TTGCTGTGAC TGCCACCCCG 
GATCAATATT CGCTGTGCGA GGTCTCGGTC ACACCTGAGG GTGTCATCAA ACAAAGACGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAT AGAATACAAT TGAGTGGAAG GTACTACTTG 
AAAAACAACA TGGAGACAGA AACTCTTTGT TCAGATGAAG ATGCTCAGGA GCTGCTGAGG 
GAGAGTCAAA TCTCCCTGTT GCAGCTCAGT ACTGTTGAAG TTGCCACACA GCTCTCTATG 
CGAAACTTTG AACTCTTCCG TAACATTGAA CCCACCGAAT ATATAGATGA TTTATTTAAA 
CTCAAATCTA AAACCAGCTG TGCCAACCTG AAGAAATTTG AAGAAGTAAT TAACCAAGAA 
ACATTTTGGG TAGCATCTGA AATTCTGAGA GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT TGCACTGCAC TGCAGGGAAT GCAAGAATTT TAACTCCATG 
TTTGCAATCA TCAGTGGCCT AAACTTGGCC CCAGTGGCAA GACTACGGAC CACCTGGGAA 
AAACTTCCCA ATAAATATGA AAAGCTGTTT CAAGATCTCC AGGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATATCG CAATGTCCTC AATAGTCAAA ACCTGCAGCC TCCCATAATC 
CCCCTATTCC CAGTTATCAA GAAGGATCTC ACATTCCTTC ATGAAGGAAA TGACTCAAAA 
GTAGATGGGT TAGTCAATTT TGAGAAGCTA AGGATGATCG CAAAAGAAAT TCGTCATGTT 
GGCCGAATGG CTTCAGTTAA CATGGACCCT GCCCTCATGT TCAGAACTCG GAAGAAGAAG 
TGGAGGAGTT TGGGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGACGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TGTCCAATTT GGAGCTAGAA 
ATGGATGAGG AGAGTCTTCA GACATTATCA CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAGTCTGAGA CGTCCCCAGT GGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC ACAAGCCCAG CCGCCGCCCC CGCAGCCACA GCCCCAACAT 
AAGATCAACC AAGGCCTTCA GGTTCCTGCT GTATCCCTTT ATCCTTCACG AAAGAAAGTA 
CCTGTAAAGG ATCTCCCACC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG TCTGGAGCGT CATAAAAAAC AAGCAGAAGA TACTATATCA 
AATGCATCCT CACAGCTTTC TTCTCCTCCC ACTTCTCCAC AAAGTTCTCC AAGGAAAGGT 
GGCAGTGGCA ATCAGCTGAG ATCTTTTGGC TCCGGGCAGT TGGACTTAAC CAGTTCCTCC 
TCTTCTCTTG GAAGTGAGAA CAGTAACAAG AATAACAATG CACCACGGAC CTATGGGATA 
GGCTACACCT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTCAGCAAT TCTTCCTTTG ACTCCGTGCC AGTCTCGCTG 
CATGAGGAGA GGCGGCAGAG GCATTCCGTC AGCATCGTGG AAACAAACCT CGGCGTGGGC 
AGGATGGAAA GGCGGACTAT GATGGAACCA GATCAGTACA GCTTAGGGTC CTATGCACCA 
ATGGCTGAGA GTCGAGGCTT ATATGCTACA GCTACGGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AAGATCAGGG GGATCGGGCT TCACTCGATG CTGCCGACAG TGGCCGTGGG 
AGCTGGACGT CGTGCTCAAG CGGTTCACAT GATAACATAC AGACCATCCA GCACCAGAGA 
AGCTGGGAAA CACTTCCATT TGGGCATACT CACTTTGACT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCCT CAAGCAGCCA TATGGACCAA ATAATGTTTT CCGATCACAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGTCTT GAACAAGCTC AGTCCCGGGC AAGCTGGGCA 
TCTTCCACAG GGTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT CAAGCGAAGG 
GGTGGAAAAG ATGTTTCCAT TGAAGCTGAA AGCAGTAGCG TTACCTCTGT GACCACAGAA 
GAAACCAAAC CTGTTCCCAT GCCTGCCCAC GTGGCTGTGA CATCGAGCAC TGCAAAGGGA 
CTTATTGTGC GGAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC AGGCTACATT 
GGAATCCCCA TCACTGACTT TCCCGAAGGA CATTCCCATC CAGCCAGGAA GCCTCCAGAC 
TACAATGTGG CACTTCAGAG ATCTCGGATG GTCGCCCGAC CCACTGACAC AGCCGCGCCT 
TCGCCCATAC AGCAGCCGCA CGGGCATCCG GCGAGCGGAA GGCCGGTGAA CAAACCACAG 
TGGCATAAAC CCAATGAGTG TGACCCGCGC CTCGCCCCCT ATCAGTCTCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTATCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974869.1
CDS	16..5094
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119394.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119394.1

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGC TTCCTGCAGA TTTCACAAAA 
CTACACCTTA CTGATAGTCT CCACCCACAG GTGACGCACG TTTCTTCTAG CCATTCAGGA 
TGTAGTATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAA 
AGTGAGGCTG GTGATATGGA CCTGAGTGGG TTGCCAGAAA CAGCAGTGGA TTCTGAGGAC 
GATGATGATG AAGAAGACAT TGAGAGGGCA TCAGATCCTC TGATGAGTAG AGATATTGTG 
AGAGACTGTC TAGAGAAGGA TCCAATTGAC AGGACAGATG ATGACATTGA ACAACTCTTA 
GAATTTATGC ACCAGTTGCC TGCTTTTGCC AATATGACAA TGTCAGTGAG ACGAGAACTC 
TGTGCCGTGA TGGTGTTTGC AGTGGTGGAA AGAGCTGGGA CCATAGTGTT AAATGATGGT 
GAAGAGCTGG ATTCCTGGTC AGTGATTCTG AATGGTTCTG TGGAAGTAAC TTATCCAGAT 
GGAAAAGCAG AAATATTATG TATGGGAAAT AGTTTTGGTG TCTCTCCTAC CATGGACAAA 
GAATACATGA AAGGAGTAAT GAGAACAAAA GTGGATGACT GCCAGTTTGT CTGTATAGCC 
CAGCAAGATT ACTGCCGTAT TCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAGGAA 
GAAGGAGAAA TTGTTATGGT AAAAGAACAC CGAGAACTTG ATAGAACTGG AACAAGAAAG 
GGACATATTG TCATCAAGGG CACCTCAGAA AGATTAACAA TGCATTTGGT GGAAGAGCAT 
TCGGTGGTGG ATCCAACATT CATAGAGGAC TTCCTGTTGA CCTATAGGAC TTTTCTTTCT 
AGCCCAATGG AAGTGGGCAA AAAGTTATTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACAC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAACC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ATTGATGACG 
TTAACAAAAC CATCTCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGTGG CTCAGAGAAG 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGGTAGCA AAGCAACTGA AGCAGGCTTG 
AAACGAGGGG ACCAGATATT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTGTCA 
AAAGCCATGG AAATTCTTAG GAATAACACA CACTTATCAA TCACTGTGAA AACCAACTTA 
TTCGTATTTA AAGAACTTCT AACAAGATTG TCAGAAGAGA AAAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTTGCTGTA 
GATGTAGAAC AGGTGATAGG ACTTGAAAAA GTAAATAAAA AAAGTAAAGC CAATACTGTT 
GGAGGAAGGA ACAAGCTAAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATTGG GATTGGTCAG TCTCAAGATG ACAGTATAGT GGGATTAAGA 
CAGACGAAGC ACATCCCAAC TGCACTGCCC GTCAGTGGAA CCTTGTCATC CAGTAATCCT 
GATTTATTAC AGTCACATCA TCGCATTCTG GACTTCAGTA CTACTCCTGA CTTGCCAGAT 
CAAGTGTTAA GGGTTTTTAA GGCTGATCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACCACAGCGA AGGAAGTAGT CATTCAGGCC ATCAGGGAGT TTGCTGTGAC TGCCACCCCG 
GATCAATATT CGCTGTGCGA GGTCTCGGTC ACACCTGAGG GTGTCATCAA ACAAAGACGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAT AGAATACAAT TGAGTGGAAG GTACTACTTG 
AAAAACAACA TGGAGACAGA AACTCTTTGT TCAGATGAAG ATGCTCAGGA GCTGCTGAGG 
GAGAGTCAAA TCTCCCTGTT GCAGCTCAGT ACTGTTGAAG TTGCCACACA GCTCTCTATG 
CGAAACTTTG AACTCTTCCG TAACATTGAA CCCACCGAAT ATATAGATGA TTTATTTAAA 
CTCAAATCTA AAACCAGCTG TGCCAACCTG AAGAAATTTG AAGAAGTAAT TAACCAAGAA 
ACATTTTGGG TAGCATCTGA AATTCTGAGA GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT TGCACTGCAC TGCAGGGAAT GCAAGAATTT TAACTCCATG 
TTTGCAATCA TCAGTGGCCT AAACTTGGCC CCAGTGGCAA GACTACGGAC CACCTGGGAA 
AAACTTCCCA ATAAATATGA AAAGCTGTTT CAAGATCTCC AGGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATATCG CAATGTCCTC AATAGTCAAA ACCTGCAGCC TCCCATAATC 
CCCCTATTCC CAGTTATCAA GAAGGATCTC ACATTCCTTC ATGAAGGAAA TGACTCAAAA 
GTAGATGGGT TAGTCAATTT TGAGAAGCTA AGGATGATCG CAAAAGAAAT TCGTCATGTT 
GGCCGAATGG CTTCAGTTAA CATGGACCCT GCCCTCATGT TCAGAACTCG GAAGAAGAAG 
TGGAGGAGTT TGGGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGACGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TGTCCAATTT GGAGCTAGAA 
ATGGATGAGG AGAGTCTTCA GACATTATCA CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAGTCTGAGA CGTCCCCAGT GGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC ACAAGCCCAG CCGCCGCCCC CGCAGCCACA GCCCCAACAT 
AAGATCAACC AAGGCCTTCA GGTTCCTGCT GTATCCCTTT ATCCTTCACG AAAGAAAGTA 
CCTGTAAAGG ATCTCCCACC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG TCTGGAGCGT CATAAAAAAC AAGCAGAAGA TACTATATCA 
AATGCATCCT CACAGCTTTC TTCTCCTCCC ACTTCTCCAC AAAGTTCTCC AAGGAAAGGT 
GGCAGTGGCA ATCAGCTGAG ATCTTTTGGC TCCGGGCAGT TGGACTTAAC CAGTTCCTCC 
TCTTCTCTTG GAAGTGAGAA CAGTAACAAG AATAACAATG CACCACGGAC CTATGGGATA 
GGCTACACCT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTCAGCAAT TCTTCCTTTG ACTCCGTGCC AGTCTCGCTG 
CATGAGGAGA GGCGGCAGAG GCATTCCGTC AGCATCGTGG AAACAAACCT CGGCGTGGGC 
AGGATGGAAA GGCGGACTAT GATGGAACCA GATCAGTACA GCTTAGGGTC CTATGCACCA 
ATGGCTGAGA GTCGAGGCTT ATATGCTACA GCTACGGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AAGATCAGGG GGATCGGGCT TCACTCGATG CTGCCGACAG TGGCCGTGGG 
AGCTGGACGT CGTGCTCAAG CGGTTCACAT GATAACATAC AGACCATCCA GCACCAGAGA 
AGCTGGGAAA CACTTCCATT TGGGCATACT CACTTTGACT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCCT CAAGCAGCCA TATGGACCAA ATAATGTTTT CCGATCACAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGTCTT GAACAAGCTC AGTCCCGGGC AAGCTGGGCA 
TCTTCCACAG GGTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT CAAGCGAAGG 
GGTGGAAAAG ATGTTTCCAT TGAAGCTGAA AGCAGTAGCG TTACCTCTGT GACCACAGAA 
GAAACCAAAC CTGTTCCCAT GCCTGCCCAC GTGGCTGTGA CATCGAGCAC TGCAAAGGGA 
CTTATTGTGC GGAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC AGGCTACATT 
GGAATCCCCA TCACTGACTT TCCCGAAGGA CATTCCCATC CAGCCAGGAA GCCTCCAGAC 
TACAATGTGG CACTTCAGAG ATCTCGGATG GTCGCCCGAC CCACTGACAC AGCCGCGCCT 
TCGCCCATAC AGCAGCCGCA CGGGCATCCG GCGAGCGGAA GGCCGGTGAA CAAACCACAG 
TGGCATAAAC CCAATGAGTG TGACCCGCGC CTCGCCCCCT ATCAGTCTCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTATCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119395.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974870.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe40185
Clone ID Related Accession (Same CDS sequence)	XM_014119395.1
Accession Version	XM_014119395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974870.1
CDS	16..5088
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119395.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119395.1

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119396.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974871.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OCe40186
Clone ID Related Accession (Same CDS sequence)	XM_014119396.1
Accession Version	XM_014119396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGCTACACCT TGGCTCCCAG TGGTACTGTG 
GATAATTTTT CAGATTCTGG TCACAGTGAA ATTTCTTCAC GATCCAGTAT TGTCAGCAAT 
TCTTCCTTTG ACTCCGTGCC AGTCTCGCTG CATGAGGAGA GGCGGCAGAG GCATTCCGTC 
AGCATCGTGG AAACAAACCT CGGCGTGGGC AGGATGGAAA GGCGGACTAT GATGGAACCA 
GATCAGTACA GCTTAGGGTC CTATGCACCA ATGGCTGAGA GTCGAGGCTT ATATGCTACA 
GCTACGGTAA TTTCTTCTCC AAGCACAGAG GAACTTTCCC AAGATCAGGG GGATCGGGCT 
TCACTCGATG CTGCCGACAG TGGCCGTGGG AGCTGGACGT CGTGCTCAAG CGGTTCACAT 
GATAACATAC AGACCATCCA GCACCAGAGA AGCTGGGAAA CACTTCCATT TGGGCATACT 
CACTTTGACT ATTCAGGGGA TCCTGCAGGT TTATGGGCCT CAAGCAGCCA TATGGACCAA 
ATAATGTTTT CCGATCACAG CACAAAGTAT AACAGGCAAA ATCAAAGTAG AGAGAGTCTT 
GAACAAGCTC AGTCCCGGGC AAGCTGGGCA TCTTCCACAG GGTACTGGGG AGAAGACTCA 
GAAGGTGACA CAGGCACAAT CAAGCGAAGG GGTGGAAAAG ATGTTTCCAT TGAAGCTGAA 
AGCAGTAGCG TTACCTCTGT GACCACAGAA GAAACCAAAC CTGTTCCCAT GCCTGCCCAC 
GTGGCTGTGA CATCGAGCAC TGCAAAGGGA CTTATTGTGC GGAAGGAGGG CAGGTATCGA 
GAGCCCCCGC CCACCCCTCC AGGCTACATT GGAATCCCCA TCACTGACTT TCCCGAAGGA 
CATTCCCATC CAGCCAGGAA GCCTCCAGAC TACAATGTGG CACTTCAGAG ATCTCGGATG 
GTCGCCCGAC CCACTGACAC AGCCGCGCCT TCGCCCATAC AGCAGCCGCA CGGGCATCCG 
GCGAGCGGAA GGCCGGTGAA CAAACCACAG TGGCATAAAC CCAATGAGTG TGACCCGCGC 
CTCGCCCCCT ATCAGTCTCA AGGGTTTTCC ACCGAGGAGG ATGAAGATGA ACAAGTATCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974871.1
CDS	16..5064
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119396.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119396.1

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGTGG CAGTGGCAAT CAGCTGAGAT CTTTTGGCTC CGGGCAGTTG 
GACTTAACCA GTTCCTCCTC TTCTCTTGGA AGCTACACCT TGGCTCCCAG TGGTACTGTG 
GATAATTTTT CAGATTCTGG TCACAGTGAA ATTTCTTCAC GATCCAGTAT TGTCAGCAAT 
TCTTCCTTTG ACTCCGTGCC AGTCTCGCTG CATGAGGAGA GGCGGCAGAG GCATTCCGTC 
AGCATCGTGG AAACAAACCT CGGCGTGGGC AGGATGGAAA GGCGGACTAT GATGGAACCA 
GATCAGTACA GCTTAGGGTC CTATGCACCA ATGGCTGAGA GTCGAGGCTT ATATGCTACA 
GCTACGGTAA TTTCTTCTCC AAGCACAGAG GAACTTTCCC AAGATCAGGG GGATCGGGCT 
TCACTCGATG CTGCCGACAG TGGCCGTGGG AGCTGGACGT CGTGCTCAAG CGGTTCACAT 
GATAACATAC AGACCATCCA GCACCAGAGA AGCTGGGAAA CACTTCCATT TGGGCATACT 
CACTTTGACT ATTCAGGGGA TCCTGCAGGT TTATGGGCCT CAAGCAGCCA TATGGACCAA 
ATAATGTTTT CCGATCACAG CACAAAGTAT AACAGGCAAA ATCAAAGTAG AGAGAGTCTT 
GAACAAGCTC AGTCCCGGGC AAGCTGGGCA TCTTCCACAG GGTACTGGGG AGAAGACTCA 
GAAGGTGACA CAGGCACAAT CAAGCGAAGG GGTGGAAAAG ATGTTTCCAT TGAAGCTGAA 
AGCAGTAGCG TTACCTCTGT GACCACAGAA GAAACCAAAC CTGTTCCCAT GCCTGCCCAC 
GTGGCTGTGA CATCGAGCAC TGCAAAGGGA CTTATTGTGC GGAAGGAGGG CAGGTATCGA 
GAGCCCCCGC CCACCCCTCC AGGCTACATT GGAATCCCCA TCACTGACTT TCCCGAAGGA 
CATTCCCATC CAGCCAGGAA GCCTCCAGAC TACAATGTGG CACTTCAGAG ATCTCGGATG 
GTCGCCCGAC CCACTGACAC AGCCGCGCCT TCGCCCATAC AGCAGCCGCA CGGGCATCCG 
GCGAGCGGAA GGCCGGTGAA CAAACCACAG TGGCATAAAC CCAATGAGTG TGACCCGCGC 
CTCGCCCCCT ATCAGTCTCA AGGGTTTTCC ACCGAGGAGG ATGAAGATGA ACAAGTATCT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005629385.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629442.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.

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CloneID	OCe40187
Clone ID Related Accession (Same CDS sequence)	XM_005629385.2
Accession Version	XM_005629385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGCTA CACCTTGGCT CCCAGTGGTA CTGTGGATAA TTTTTCAGAT 
TCTGGTCACA GTGAAATTTC TTCACGATCC AGTATTGTCA GCAATTCTTC CTTTGACTCC 
GTGCCAGTCT CGCTGCATGA GGAGAGGCGG CAGAGGCATT CCGTCAGCAT CGTGGAAACA 
AACCTCGGCG TGGGCAGGAT GGAAAGGCGG ACTATGATGG AACCAGATCA GTACAGCTTA 
GGGTCCTATG CACCAATGGC TGAGAGTCGA GGCTTATATG CTACAGCTAC GGTAATTTCT 
TCTCCAAGCA CAGAGGAACT TTCCCAAGAT CAGGGGGATC GGGCTTCACT CGATGCTGCC 
GACAGTGGCC GTGGGAGCTG GACGTCGTGC TCAAGCGGTT CACATGATAA CATACAGACC 
ATCCAGCACC AGAGAAGCTG GGAAACACTT CCATTTGGGC ATACTCACTT TGACTATTCA 
GGGGATCCTG CAGGTTTATG GGCCTCAAGC AGCCATATGG ACCAAATAAT GTTTTCCGAT 
CACAGCACAA AGTATAACAG GCAAAATCAA AGTAGAGAGA GTCTTGAACA AGCTCAGTCC 
CGGGCAAGCT GGGCATCTTC CACAGGGTAC TGGGGAGAAG ACTCAGAAGG TGACACAGGC 
ACAATCAAGC GAAGGGGTGG AAAAGATGTT TCCATTGAAG CTGAAAGCAG TAGCGTTACC 
TCTGTGACCA CAGAAGAAAC CAAACCTGTT CCCATGCCTG CCCACGTGGC TGTGACATCG 
AGCACTGCAA AGGGACTTAT TGTGCGGAAG GAGGGCAGGT ATCGAGAGCC CCCGCCCACC 
CCTCCAGGCT ACATTGGAAT CCCCATCACT GACTTTCCCG AAGGACATTC CCATCCAGCC 
AGGAAGCCTC CAGACTACAA TGTGGCACTT CAGAGATCTC GGATGGTCGC CCGACCCACT 
GACACAGCCG CGCCTTCGCC CATACAGCAG CCGCACGGGC ATCCGGCGAG CGGAAGGCCG 
GTGAACAAAC CACAGTGGCA TAAACCCAAT GAGTGTGACC CGCGCCTCGC CCCCTATCAG 
TCTCAAGGGT TTTCCACCGA GGAGGATGAA GATGAACAAG TATCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629442.2
CDS	16..4989
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005629385.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629385.2

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGG AATGCACTAA ATCTCAGCTT 
CCTGCAGATT TCACAAAACT ACACCTTACT GATAGTCTCC ACCCACAGGT GACGCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GAAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG TGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CTGAGGACGA TGATGATGAA GAAGACATTG AGAGGGCATC AGATCCTCTG 
ATGAGTAGAG ATATTGTGAG AGACTGTCTA GAGAAGGATC CAATTGACAG GACAGATGAT 
GACATTGAAC AACTCTTAGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGAC GAGAACTCTG TGCCGTGATG GTGTTTGCAG TGGTGGAAAG AGCTGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAT TCCTGGTCAG TGATTCTGAA TGGTTCTGTG 
GAAGTAACTT ATCCAGATGG AAAAGCAGAA ATATTATGTA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTAATGA GAACAAAAGT GGATGACTGC 
CAGTTTGTCT GTATAGCCCA GCAAGATTAC TGCCGTATTC TCAACCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAGGAAGA AGGAGAAATT GTTATGGTAA AAGAACACCG AGAACTTGAT 
AGAACTGGAA CAAGAAAGGG ACATATTGTC ATCAAGGGCA CCTCAGAAAG ATTAACAATG 
CATTTGGTGG AAGAGCATTC GGTGGTGGAT CCAACATTCA TAGAGGACTT CCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTGAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTCTAG AAGAATTTGA AAATAACCTG 
GAAAGAGAGA AAATGGGTGG ACATCTAAGG CTGTTAAATA TTGCATGTGC AGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCTCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGTGGCT CAGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGAGGGGAC CAGATATTAG AAGTAAATGG TCAAAACTTT 
GAAAATATTC AGCTGTCAAA AGCCATGGAA ATTCTTAGGA ATAACACACA CTTATCAATC 
ACTGTGAAAA CCAACTTATT CGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCTCACCT CCCTAAAATT GGAGACATCA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT AAATAAAAAA 
AGTAAAGCCA ATACTGTTGG AGGAAGGAAC AAGCTAAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATATAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGTATAGTGG GATTAAGACA GACGAAGCAC ATCCCAACTG CACTGCCCGT CAGTGGAACC 
TTGTCATCCA GTAATCCTGA TTTATTACAG TCACATCATC GCATTCTGGA CTTCAGTACT 
ACTCCTGACT TGCCAGATCA AGTGTTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGATAC 
ATCATGATCA GTAAGGACAC CACAGCGAAG GAAGTAGTCA TTCAGGCCAT CAGGGAGTTT 
GCTGTGACTG CCACCCCGGA TCAATATTCG CTGTGCGAGG TCTCGGTCAC ACCTGAGGGT 
GTCATCAAAC AAAGACGGCT TCCAGATCAG CTTTCCAAAC TTGCTGATAG AATACAATTG 
AGTGGAAGGT ACTACTTGAA AAACAACATG GAGACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGC TGCTGAGGGA GAGTCAAATC TCCCTGTTGC AGCTCAGTAC TGTTGAAGTT 
GCCACACAGC TCTCTATGCG AAACTTTGAA CTCTTCCGTA ACATTGAACC CACCGAATAT 
ATAGATGATT TATTTAAACT CAAATCTAAA ACCAGCTGTG CCAACCTGAA GAAATTTGAA 
GAAGTAATTA ACCAAGAAAC ATTTTGGGTA GCATCTGAAA TTCTGAGAGA GACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATTG CACTGCACTG CAGGGAATGC 
AAGAATTTTA ACTCCATGTT TGCAATCATC AGTGGCCTAA ACTTGGCCCC AGTGGCAAGA 
CTACGGACCA CCTGGGAAAA ACTTCCCAAT AAATATGAAA AGCTGTTTCA AGATCTCCAG 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGCA ATGTCCTCAA TAGTCAAAAC 
CTGCAGCCTC CCATAATCCC CCTATTCCCA GTTATCAAGA AGGATCTCAC ATTCCTTCAT 
GAAGGAAATG ACTCAAAAGT AGATGGGTTA GTCAATTTTG AGAAGCTAAG GATGATCGCA 
AAAGAAATTC GTCATGTTGG CCGAATGGCT TCAGTTAACA TGGACCCTGC CCTCATGTTC 
AGAACTCGGA AGAAGAAGTG GAGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ACGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTG 
TCCAATTTGG AGCTAGAAAT GGATGAGGAG AGTCTTCAGA CATTATCACT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA GTCTGAGACG 
TCCCCAGTGG CTCCAAGGGC AGGGTCACAA CAGAAAGCAC AAGCCCAGCC GCCGCCCCCG 
CAGCCACAGC CCCAACATAA GATCAACCAA GGCCTTCAGG TTCCTGCTGT ATCCCTTTAT 
CCTTCACGAA AGAAAGTACC TGTAAAGGAT CTCCCACCTT TTGGCATAAA CTCTCCACAA 
GCTTTAAAGA AAATTCTTTC TTTGTCTGAA GAAGGAAGTC TGGAGCGTCA TAAAAAACAA 
GCAGAAGATA CTATATCAAA TGCATCCTCA CAGCTTTCTT CTCCTCCCAC TTCTCCACAA 
AGTTCTCCAA GGAAAGGCTA CACCTTGGCT CCCAGTGGTA CTGTGGATAA TTTTTCAGAT 
TCTGGTCACA GTGAAATTTC TTCACGATCC AGTATTGTCA GCAATTCTTC CTTTGACTCC 
GTGCCAGTCT CGCTGCATGA GGAGAGGCGG CAGAGGCATT CCGTCAGCAT CGTGGAAACA 
AACCTCGGCG TGGGCAGGAT GGAAAGGCGG ACTATGATGG AACCAGATCA GTACAGCTTA 
GGGTCCTATG CACCAATGGC TGAGAGTCGA GGCTTATATG CTACAGCTAC GGTAATTTCT 
TCTCCAAGCA CAGAGGAACT TTCCCAAGAT CAGGGGGATC GGGCTTCACT CGATGCTGCC 
GACAGTGGCC GTGGGAGCTG GACGTCGTGC TCAAGCGGTT CACATGATAA CATACAGACC 
ATCCAGCACC AGAGAAGCTG GGAAACACTT CCATTTGGGC ATACTCACTT TGACTATTCA 
GGGGATCCTG CAGGTTTATG GGCCTCAAGC AGCCATATGG ACCAAATAAT GTTTTCCGAT 
CACAGCACAA AGTATAACAG GCAAAATCAA AGTAGAGAGA GTCTTGAACA AGCTCAGTCC 
CGGGCAAGCT GGGCATCTTC CACAGGGTAC TGGGGAGAAG ACTCAGAAGG TGACACAGGC 
ACAATCAAGC GAAGGGGTGG AAAAGATGTT TCCATTGAAG CTGAAAGCAG TAGCGTTACC 
TCTGTGACCA CAGAAGAAAC CAAACCTGTT CCCATGCCTG CCCACGTGGC TGTGACATCG 
AGCACTGCAA AGGGACTTAT TGTGCGGAAG GAGGGCAGGT ATCGAGAGCC CCCGCCCACC 
CCTCCAGGCT ACATTGGAAT CCCCATCACT GACTTTCCCG AAGGACATTC CCATCCAGCC 
AGGAAGCCTC CAGACTACAA TGTGGCACTT CAGAGATCTC GGATGGTCGC CCGACCCACT 
GACACAGCCG CGCCTTCGCC CATACAGCAG CCGCACGGGC ATCCGGCGAG CGGAAGGCCG 
GTGAACAAAC CACAGTGGCA TAAACCCAAT GAGTGTGACC CGCGCCTCGC CCCCTATCAG 
TCTCAAGGGT TTTCCACCGA GGAGGATGAA GATGAACAAG TATCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119397.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974872.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.

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CloneID	OCe40188
Clone ID Related Accession (Same CDS sequence)	XM_014119397.1
Accession Version	XM_014119397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGC TTCCTGCAGA TTTCACAAAA 
CTACACCTTA CTGATAGTCT CCACCCACAG GTGACGCACG TTTCTTCTAG CCATTCAGGA 
TGTAGTATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAA 
AGTGAGGCTG GTGATATGGA CCTGAGTGGG TTGCCAGAAA CAGCAGTGGA TTCTGAGGAC 
GATGATGATG AAGAAGACAT TGAGAGGGCA TCAGATCCTC TGATGAGTAG AGATATTGTG 
AGAGACTGTC TAGAGAAGGA TCCAATTGAC AGGACAGATG ATGACATTGA ACAACTCTTA 
GAATTTATGC ACCAGTTGCC TGCTTTTGCC AATATGACAA TGTCAGTGAG ACGAGAACTC 
TGTGCCGTGA TGGTGTTTGC AGTGGTGGAA AGAGCTGGGA CCATAGTGTT AAATGATGGT 
GAAGAGCTGG ATTCCTGGTC AGTGATTCTG AATGGTTCTG TGGAAGTAAC TTATCCAGAT 
GGAAAAGCAG AAATATTATG TATGGGAAAT AGTTTTGGTG TCTCTCCTAC CATGGACAAA 
GAATACATGA AAGGAGTAAT GAGAACAAAA GTGGATGACT GCCAGTTTGT CTGTATAGCC 
CAGCAAGATT ACTGCCGTAT TCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAGGAA 
GAAGGAGAAA TTGTTATGGT AAAAGAACAC CGAGAACTTG ATAGAACTGG AACAAGAAAG 
GGACATATTG TCATCAAGGG CACCTCAGAA AGATTAACAA TGCATTTGGT GGAAGAGCAT 
TCGGTGGTGG ATCCAACATT CATAGAGGAC TTCCTGTTGA CCTATAGGAC TTTTCTTTCT 
AGCCCAATGG AAGTGGGCAA AAAGTTATTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACAC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAACC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ATTGATGACG 
TTAACAAAAC CATCTCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGTGG CTCAGAGAAG 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGGTAGCA AAGCAACTGA AGCAGGCTTG 
AAACGAGGGG ACCAGATATT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTGTCA 
AAAGCCATGG AAATTCTTAG GAATAACACA CACTTATCAA TCACTGTGAA AACCAACTTA 
TTCGTATTTA AAGAACTTCT AACAAGATTG TCAGAAGAGA AAAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTTGCTGTA 
GATGTAGAAC AGGTGATAGG ACTTGAAAAA GTAAATAAAA AAAGTAAAGC CAATACTGTT 
GGAGGAAGGA ACAAGCTAAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATTGG GATTGGTCAG TCTCAAGATG ACAGTATAGT GGGATTAAGA 
CAGACGAAGC ACATCCCAAC TGCACTGCCC GTCAGTGGAA CCTTGTCATC CAGTAATCCT 
GATTTATTAC AGTCACATCA TCGCATTCTG GACTTCAGTA CTACTCCTGA CTTGCCAGAT 
CAAGTGTTAA GGGTTTTTAA GGCTGATCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACCACAGCGA AGGAAGTAGT CATTCAGGCC ATCAGGGAGT TTGCTGTGAC TGCCACCCCG 
GATCAATATT CGCTGTGCGA GGTCTCGGTC ACACCTGAGG GTGTCATCAA ACAAAGACGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAT AGAATACAAT TGAGTGGAAG GTACTACTTG 
AAAAACAACA TGGAGACAGA AACTCTTTGT TCAGATGAAG ATGCTCAGGA GCTGCTGAGG 
GAGAGTCAAA TCTCCCTGTT GCAGCTCAGT ACTGTTGAAG TTGCCACACA GCTCTCTATG 
CGAAACTTTG AACTCTTCCG TAACATTGAA CCCACCGAAT ATATAGATGA TTTATTTAAA 
CTCAAATCTA AAACCAGCTG TGCCAACCTG AAGAAATTTG AAGAAGTAAT TAACCAAGAA 
ACATTTTGGG TAGCATCTGA AATTCTGAGA GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT TGCACTGCAC TGCAGGGAAT GCAAGAATTT TAACTCCATG 
TTTGCAATCA TCAGTGGCCT AAACTTGGCC CCAGTGGCAA GACTACGGAC CACCTGGGAA 
AAACTTCCCA ATAAATATGA AAAGCTGTTT CAAGATCTCC AGGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATATCG CAATGTCCTC AATAGTCAAA ACCTGCAGCC TCCCATAATC 
CCCCTATTCC CAGTTATCAA GAAGGATCTC ACATTCCTTC ATGAAGGAAA TGACTCAAAA 
GTAGATGGGT TAGTCAATTT TGAGAAGCTA AGGATGATCG CAAAAGAAAT TCGTCATGTT 
GGCCGAATGG CTTCAGTTAA CATGGACCCT GCCCTCATGT TCAGAACTCG GAAGAAGAAG 
TGGAGGAGTT TGGGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGACGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TGTCCAATTT GGAGCTAGAA 
ATGGATGAGG AGAGTCTTCA GACATTATCA CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAGTCTGAGA CGTCCCCAGT GGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC ACAAGCCCAG CCGCCGCCCC CGCAGCCACA GCCCCAACAT 
AAGATCAACC AAGGCCTTCA GGTTCCTGCT GTATCCCTTT ATCCTTCACG AAAGAAAGTA 
CCTGTAAAGG ATCTCCCACC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG TCTGGAGCGT CATAAAAAAC AAGCAGAAGA TACTATATCA 
AATGCATCCT CACAGCTTTC TTCTCCTCCC ACTTCTCCAC AAAGTTCTCC AAGGAAAGGC 
TACACCTTGG CTCCCAGTGG TACTGTGGAT AATTTTTCAG ATTCTGGTCA CAGTGAAATT 
TCTTCACGAT CCAGTATTGT CAGCAATTCT TCCTTTGACT CCGTGCCAGT CTCGCTGCAT 
GAGGAGAGGC GGCAGAGGCA TTCCGTCAGC ATCGTGGAAA CAAACCTCGG CGTGGGCAGG 
ATGGAAAGGC GGACTATGAT GGAACCAGAT CAGTACAGCT TAGGGTCCTA TGCACCAATG 
GCTGAGAGTC GAGGCTTATA TGCTACAGCT ACGGTAATTT CTTCTCCAAG CACAGAGGAA 
CTTTCCCAAG ATCAGGGGGA TCGGGCTTCA CTCGATGCTG CCGACAGTGG CCGTGGGAGC 
TGGACGTCGT GCTCAAGCGG TTCACATGAT AACATACAGA CCATCCAGCA CCAGAGAAGC 
TGGGAAACAC TTCCATTTGG GCATACTCAC TTTGACTATT CAGGGGATCC TGCAGGTTTA 
TGGGCCTCAA GCAGCCATAT GGACCAAATA ATGTTTTCCG ATCACAGCAC AAAGTATAAC 
AGGCAAAATC AAAGTAGAGA GAGTCTTGAA CAAGCTCAGT CCCGGGCAAG CTGGGCATCT 
TCCACAGGGT ACTGGGGAGA AGACTCAGAA GGTGACACAG GCACAATCAA GCGAAGGGGT 
GGAAAAGATG TTTCCATTGA AGCTGAAAGC AGTAGCGTTA CCTCTGTGAC CACAGAAGAA 
ACCAAACCTG TTCCCATGCC TGCCCACGTG GCTGTGACAT CGAGCACTGC AAAGGGACTT 
ATTGTGCGGA AGGAGGGCAG GTATCGAGAG CCCCCGCCCA CCCCTCCAGG CTACATTGGA 
ATCCCCATCA CTGACTTTCC CGAAGGACAT TCCCATCCAG CCAGGAAGCC TCCAGACTAC 
AATGTGGCAC TTCAGAGATC TCGGATGGTC GCCCGACCCA CTGACACAGC CGCGCCTTCG 
CCCATACAGC AGCCGCACGG GCATCCGGCG AGCGGAAGGC CGGTGAACAA ACCACAGTGG 
CATAAACCCA ATGAGTGTGA CCCGCGCCTC GCCCCCTATC AGTCTCAAGG GTTTTCCACC 
GAGGAGGATG AAGATGAACA AGTATCTGCT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974872.1
CDS	16..4971
Translation	MRIFLFCCPKRCEFWEAVNLVDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119397.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119397.1

ORF Insert Sequence:

ATGAGGATAT TTTTGTTTTG CTGCCCTAAG AGATGTGAAT TCTGGGAAGC TGTCAACTTG 
GTGGATCTGG AAATAGTATA TTCCTATTTA CATGGTATGG AAGCCTTATC AAACTTGAGA 
GAGCACCAGC TTAGGTTAAT GTGTGAAACT GTGAGATATG AAAGACATGA AGCAAATGAA 
GTTTTATACT ATCCTGATGA TATTGGGACC TGCTGGTATA TCCTTCTCTC TGGTTCCGTG 
TTCATTAAGG AATCCATGTT TCTTCCAAGA AGCAGTTTTG GCAAGCGTTC TGCAGGAAGC 
TTTAGGCGTG GCTGTGAATG CATTGTTTTA GAGCCTTCTG AAATGATTGT GGTGGACTAT 
ATGGATGAAA ATGAAGAATA TTTTCAGCGT CAAGCTTCTC ATCGACAGTC TCGAAGGAGA 
TTTAGAAAAA TCAACCAGAA AGGTGAAAGA CAAACAATTA TTGACACTGT GGATCCTTAT 
CCTGTGGGCA AACCACCTTT GCCTCGAGGC TATCATACGC TTCCTGCAGA TTTCACAAAA 
CTACACCTTA CTGATAGTCT CCACCCACAG GTGACGCACG TTTCTTCTAG CCATTCAGGA 
TGTAGTATCA CTAGTGATTC TGGAAGCAGC AGTCTTTCTG ATATCTACCA GGCCACAGAA 
AGTGAGGCTG GTGATATGGA CCTGAGTGGG TTGCCAGAAA CAGCAGTGGA TTCTGAGGAC 
GATGATGATG AAGAAGACAT TGAGAGGGCA TCAGATCCTC TGATGAGTAG AGATATTGTG 
AGAGACTGTC TAGAGAAGGA TCCAATTGAC AGGACAGATG ATGACATTGA ACAACTCTTA 
GAATTTATGC ACCAGTTGCC TGCTTTTGCC AATATGACAA TGTCAGTGAG ACGAGAACTC 
TGTGCCGTGA TGGTGTTTGC AGTGGTGGAA AGAGCTGGGA CCATAGTGTT AAATGATGGT 
GAAGAGCTGG ATTCCTGGTC AGTGATTCTG AATGGTTCTG TGGAAGTAAC TTATCCAGAT 
GGAAAAGCAG AAATATTATG TATGGGAAAT AGTTTTGGTG TCTCTCCTAC CATGGACAAA 
GAATACATGA AAGGAGTAAT GAGAACAAAA GTGGATGACT GCCAGTTTGT CTGTATAGCC 
CAGCAAGATT ACTGCCGTAT TCTCAACCAA GTAGAAAAGA ACATGCAAAA AGTTGAGGAA 
GAAGGAGAAA TTGTTATGGT AAAAGAACAC CGAGAACTTG ATAGAACTGG AACAAGAAAG 
GGACATATTG TCATCAAGGG CACCTCAGAA AGATTAACAA TGCATTTGGT GGAAGAGCAT 
TCGGTGGTGG ATCCAACATT CATAGAGGAC TTCCTGTTGA CCTATAGGAC TTTTCTTTCT 
AGCCCAATGG AAGTGGGCAA AAAGTTATTG GAGTGGTTTA ATGACCCGAG CCTCAGGGAT 
AAGGTTACAC GGGTAGTATT ATTGTGGGTG AATAATCACT TCAATGACTT TGAAGGAGAT 
CCTGCAATGA CTCGATTTCT AGAAGAATTT GAAAATAACC TGGAAAGAGA GAAAATGGGT 
GGACATCTAA GGCTGTTAAA TATTGCATGT GCAGCTAAAG CAAAAAGAAG ATTGATGACG 
TTAACAAAAC CATCTCGAGA AGCTCCTTTG CCTTTTATCT TACTTGGTGG CTCAGAGAAG 
GGATTTGGAA TCTTTGTTGA CAGTGTAGAT TCAGGTAGCA AAGCAACTGA AGCAGGCTTG 
AAACGAGGGG ACCAGATATT AGAAGTAAAT GGTCAAAACT TTGAAAATAT TCAGCTGTCA 
AAAGCCATGG AAATTCTTAG GAATAACACA CACTTATCAA TCACTGTGAA AACCAACTTA 
TTCGTATTTA AAGAACTTCT AACAAGATTG TCAGAAGAGA AAAGAAATGG TGCCCCTCAC 
CTCCCTAAAA TTGGAGACAT CAAAAAGGCC AGTCGCTACT CCATTCCAGA TCTTGCTGTA 
GATGTAGAAC AGGTGATAGG ACTTGAAAAA GTAAATAAAA AAAGTAAAGC CAATACTGTT 
GGAGGAAGGA ACAAGCTAAA AAAGATACTC GACAAGACTC GGATCAGTAT CTTGCCACAG 
AAACCATATA ATGATATTGG GATTGGTCAG TCTCAAGATG ACAGTATAGT GGGATTAAGA 
CAGACGAAGC ACATCCCAAC TGCACTGCCC GTCAGTGGAA CCTTGTCATC CAGTAATCCT 
GATTTATTAC AGTCACATCA TCGCATTCTG GACTTCAGTA CTACTCCTGA CTTGCCAGAT 
CAAGTGTTAA GGGTTTTTAA GGCTGATCAG CAAAGCCGAT ACATCATGAT CAGTAAGGAC 
ACCACAGCGA AGGAAGTAGT CATTCAGGCC ATCAGGGAGT TTGCTGTGAC TGCCACCCCG 
GATCAATATT CGCTGTGCGA GGTCTCGGTC ACACCTGAGG GTGTCATCAA ACAAAGACGG 
CTTCCAGATC AGCTTTCCAA ACTTGCTGAT AGAATACAAT TGAGTGGAAG GTACTACTTG 
AAAAACAACA TGGAGACAGA AACTCTTTGT TCAGATGAAG ATGCTCAGGA GCTGCTGAGG 
GAGAGTCAAA TCTCCCTGTT GCAGCTCAGT ACTGTTGAAG TTGCCACACA GCTCTCTATG 
CGAAACTTTG AACTCTTCCG TAACATTGAA CCCACCGAAT ATATAGATGA TTTATTTAAA 
CTCAAATCTA AAACCAGCTG TGCCAACCTG AAGAAATTTG AAGAAGTAAT TAACCAAGAA 
ACATTTTGGG TAGCATCTGA AATTCTGAGA GAGACAAACC AGCTGAAGAG GATGAAGATC 
ATTAAGCATT TCATCAAGAT TGCACTGCAC TGCAGGGAAT GCAAGAATTT TAACTCCATG 
TTTGCAATCA TCAGTGGCCT AAACTTGGCC CCAGTGGCAA GACTACGGAC CACCTGGGAA 
AAACTTCCCA ATAAATATGA AAAGCTGTTT CAAGATCTCC AGGACCTGTT TGATCCTTCC 
AGAAACATGG CAAAATATCG CAATGTCCTC AATAGTCAAA ACCTGCAGCC TCCCATAATC 
CCCCTATTCC CAGTTATCAA GAAGGATCTC ACATTCCTTC ATGAAGGAAA TGACTCAAAA 
GTAGATGGGT TAGTCAATTT TGAGAAGCTA AGGATGATCG CAAAAGAAAT TCGTCATGTT 
GGCCGAATGG CTTCAGTTAA CATGGACCCT GCCCTCATGT TCAGAACTCG GAAGAAGAAG 
TGGAGGAGTT TGGGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGACGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TGTCCAATTT GGAGCTAGAA 
ATGGATGAGG AGAGTCTTCA GACATTATCA CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAGTCTGAGA CGTCCCCAGT GGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC ACAAGCCCAG CCGCCGCCCC CGCAGCCACA GCCCCAACAT 
AAGATCAACC AAGGCCTTCA GGTTCCTGCT GTATCCCTTT ATCCTTCACG AAAGAAAGTA 
CCTGTAAAGG ATCTCCCACC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATTCTT 
TCTTTGTCTG AAGAAGGAAG TCTGGAGCGT CATAAAAAAC AAGCAGAAGA TACTATATCA 
AATGCATCCT CACAGCTTTC TTCTCCTCCC ACTTCTCCAC AAAGTTCTCC AAGGAAAGGC 
TACACCTTGG CTCCCAGTGG TACTGTGGAT AATTTTTCAG ATTCTGGTCA CAGTGAAATT 
TCTTCACGAT CCAGTATTGT CAGCAATTCT TCCTTTGACT CCGTGCCAGT CTCGCTGCAT 
GAGGAGAGGC GGCAGAGGCA TTCCGTCAGC ATCGTGGAAA CAAACCTCGG CGTGGGCAGG 
ATGGAAAGGC GGACTATGAT GGAACCAGAT CAGTACAGCT TAGGGTCCTA TGCACCAATG 
GCTGAGAGTC GAGGCTTATA TGCTACAGCT ACGGTAATTT CTTCTCCAAG CACAGAGGAA 
CTTTCCCAAG ATCAGGGGGA TCGGGCTTCA CTCGATGCTG CCGACAGTGG CCGTGGGAGC 
TGGACGTCGT GCTCAAGCGG TTCACATGAT AACATACAGA CCATCCAGCA CCAGAGAAGC 
TGGGAAACAC TTCCATTTGG GCATACTCAC TTTGACTATT CAGGGGATCC TGCAGGTTTA 
TGGGCCTCAA GCAGCCATAT GGACCAAATA ATGTTTTCCG ATCACAGCAC AAAGTATAAC 
AGGCAAAATC AAAGTAGAGA GAGTCTTGAA CAAGCTCAGT CCCGGGCAAG CTGGGCATCT 
TCCACAGGGT ACTGGGGAGA AGACTCAGAA GGTGACACAG GCACAATCAA GCGAAGGGGT 
GGAAAAGATG TTTCCATTGA AGCTGAAAGC AGTAGCGTTA CCTCTGTGAC CACAGAAGAA 
ACCAAACCTG TTCCCATGCC TGCCCACGTG GCTGTGACAT CGAGCACTGC AAAGGGACTT 
ATTGTGCGGA AGGAGGGCAG GTATCGAGAG CCCCCGCCCA CCCCTCCAGG CTACATTGGA 
ATCCCCATCA CTGACTTTCC CGAAGGACAT TCCCATCCAG CCAGGAAGCC TCCAGACTAC 
AATGTGGCAC TTCAGAGATC TCGGATGGTC GCCCGACCCA CTGACACAGC CGCGCCTTCG 
CCCATACAGC AGCCGCACGG GCATCCGGCG AGCGGAAGGC CGGTGAACAA ACCACAGTGG 
CATAAACCCA ATGAGTGTGA CCCGCGCCTC GCCCCCTATC AGTCTCAAGG GTTTTCCACC 
GAGGAGGATG AAGATGAACA AGTATCTGCT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119398.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974873.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

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CloneID	OCe40189
Clone ID Related Accession (Same CDS sequence)	XM_014119398.2 , XM_014119398.1
Accession Version	XM_014119398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTTTTAGAGC CTTCTGAAAT GATTGTGGTG GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGT 
GAAAGACAAA CAATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
CGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAACTACAC 
CTTACTGATA GTCTCCACCC ACAGGTGACG CACGTTTCTT CTAGCCATTC AGGATGTAGT 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAAAGTGAG 
GCTGGTGATA TGGACCTGAG TGGGTTGCCA GAAACAGCAG TGGATTCTGA GGACGATGAT 
GATGAAGAAG ACATTGAGAG GGCATCAGAT CCTCTGATGA GTAGAGATAT TGTGAGAGAC 
TGTCTAGAGA AGGATCCAAT TGACAGGACA GATGATGACA TTGAACAACT CTTAGAATTT 
ATGCACCAGT TGCCTGCTTT TGCCAATATG ACAATGTCAG TGAGACGAGA ACTCTGTGCC 
GTGATGGTGT TTGCAGTGGT GGAAAGAGCT GGGACCATAG TGTTAAATGA TGGTGAAGAG 
CTGGATTCCT GGTCAGTGAT TCTGAATGGT TCTGTGGAAG TAACTTATCC AGATGGAAAA 
GCAGAAATAT TATGTATGGG AAATAGTTTT GGTGTCTCTC CTACCATGGA CAAAGAATAC 
ATGAAAGGAG TAATGAGAAC AAAAGTGGAT GACTGCCAGT TTGTCTGTAT AGCCCAGCAA 
GATTACTGCC GTATTCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA GGAAGAAGGA 
GAAATTGTTA TGGTAAAAGA ACACCGAGAA CTTGATAGAA CTGGAACAAG AAAGGGACAT 
ATTGTCATCA AGGGCACCTC AGAAAGATTA ACAATGCATT TGGTGGAAGA GCATTCGGTG 
GTGGATCCAA CATTCATAGA GGACTTCCTG TTGACCTATA GGACTTTTCT TTCTAGCCCA 
ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACACGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AACCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGATTGAT GACGTTAACA 
AAACCATCTC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GTGGCTCAGA GAAGGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGGT AGCAAAGCAA CTGAAGCAGG CTTGAAACGA 
GGGGACCAGA TATTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT GTCAAAAGCC 
ATGGAAATTC TTAGGAATAA CACACACTTA TCAATCACTG TGAAAACCAA CTTATTCGTA 
TTTAAAGAAC TTCTAACAAG ATTGTCAGAA GAGAAAAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTTGC TGTAGATGTA 
GAACAGGTGA TAGGACTTGA AAAAGTAAAT AAAAAAAGTA AAGCCAATAC TGTTGGAGGA 
AGGAACAAGC TAAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TTGGGATTGG TCAGTCTCAA GATGACAGTA TAGTGGGATT AAGACAGACG 
AAGCACATCC CAACTGCACT GCCCGTCAGT GGAACCTTGT CATCCAGTAA TCCTGATTTA 
TTACAGTCAC ATCATCGCAT TCTGGACTTC AGTACTACTC CTGACTTGCC AGATCAAGTG 
TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC CGATACATCA TGATCAGTAA GGACACCACA 
GCGAAGGAAG TAGTCATTCA GGCCATCAGG GAGTTTGCTG TGACTGCCAC CCCGGATCAA 
TATTCGCTGT GCGAGGTCTC GGTCACACCT GAGGGTGTCA TCAAACAAAG ACGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGATAGAATA CAATTGAGTG GAAGGTACTA CTTGAAAAAC 
AACATGGAGA CAGAAACTCT TTGTTCAGAT GAAGATGCTC AGGAGCTGCT GAGGGAGAGT 
CAAATCTCCC TGTTGCAGCT CAGTACTGTT GAAGTTGCCA CACAGCTCTC TATGCGAAAC 
TTTGAACTCT TCCGTAACAT TGAACCCACC GAATATATAG ATGATTTATT TAAACTCAAA 
TCTAAAACCA GCTGTGCCAA CCTGAAGAAA TTTGAAGAAG TAATTAACCA AGAAACATTT 
TGGGTAGCAT CTGAAATTCT GAGAGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATTGCACT GCACTGCAGG GAATGCAAGA ATTTTAACTC CATGTTTGCA 
ATCATCAGTG GCCTAAACTT GGCCCCAGTG GCAAGACTAC GGACCACCTG GGAAAAACTT 
CCCAATAAAT ATGAAAAGCT GTTTCAAGAT CTCCAGGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ATCGCAATGT CCTCAATAGT CAAAACCTGC AGCCTCCCAT AATCCCCCTA 
TTCCCAGTTA TCAAGAAGGA TCTCACATTC CTTCATGAAG GAAATGACTC AAAAGTAGAT 
GGGTTAGTCA ATTTTGAGAA GCTAAGGATG ATCGCAAAAG AAATTCGTCA TGTTGGCCGA 
ATGGCTTCAG TTAACATGGA CCCTGCCCTC ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG 
AGTTTGGGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974873.1
CDS	152..4984
Translation	MKTSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119398.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119398.2

ORF Insert Sequence:

ATGAAAACTA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTTTTAGAGC CTTCTGAAAT GATTGTGGTG GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGT 
GAAAGACAAA CAATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
CGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAACTACAC 
CTTACTGATA GTCTCCACCC ACAGGTGACG CACGTTTCTT CTAGCCATTC AGGATGTAGT 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAAAGTGAG 
GCTGGTGATA TGGACCTGAG TGGGTTGCCA GAAACAGCAG TGGATTCTGA GGACGATGAT 
GATGAAGAAG ACATTGAGAG GGCATCAGAT CCTCTGATGA GTAGAGATAT TGTGAGAGAC 
TGTCTAGAGA AGGATCCAAT TGACAGGACA GATGATGACA TTGAACAACT CTTAGAATTT 
ATGCACCAGT TGCCTGCTTT TGCCAATATG ACAATGTCAG TGAGACGAGA ACTCTGTGCC 
GTGATGGTGT TTGCAGTGGT GGAAAGAGCT GGGACCATAG TGTTAAATGA TGGTGAAGAG 
CTGGATTCCT GGTCAGTGAT TCTGAATGGT TCTGTGGAAG TAACTTATCC AGATGGAAAA 
GCAGAAATAT TATGTATGGG AAATAGTTTT GGTGTCTCTC CTACCATGGA CAAAGAATAC 
ATGAAAGGAG TAATGAGAAC AAAAGTGGAT GACTGCCAGT TTGTCTGTAT AGCCCAGCAA 
GATTACTGCC GTATTCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA GGAAGAAGGA 
GAAATTGTTA TGGTAAAAGA ACACCGAGAA CTTGATAGAA CTGGAACAAG AAAGGGACAT 
ATTGTCATCA AGGGCACCTC AGAAAGATTA ACAATGCATT TGGTGGAAGA GCATTCGGTG 
GTGGATCCAA CATTCATAGA GGACTTCCTG TTGACCTATA GGACTTTTCT TTCTAGCCCA 
ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACACGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AACCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGATTGAT GACGTTAACA 
AAACCATCTC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GTGGCTCAGA GAAGGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGGT AGCAAAGCAA CTGAAGCAGG CTTGAAACGA 
GGGGACCAGA TATTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT GTCAAAAGCC 
ATGGAAATTC TTAGGAATAA CACACACTTA TCAATCACTG TGAAAACCAA CTTATTCGTA 
TTTAAAGAAC TTCTAACAAG ATTGTCAGAA GAGAAAAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTTGC TGTAGATGTA 
GAACAGGTGA TAGGACTTGA AAAAGTAAAT AAAAAAAGTA AAGCCAATAC TGTTGGAGGA 
AGGAACAAGC TAAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TTGGGATTGG TCAGTCTCAA GATGACAGTA TAGTGGGATT AAGACAGACG 
AAGCACATCC CAACTGCACT GCCCGTCAGT GGAACCTTGT CATCCAGTAA TCCTGATTTA 
TTACAGTCAC ATCATCGCAT TCTGGACTTC AGTACTACTC CTGACTTGCC AGATCAAGTG 
TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC CGATACATCA TGATCAGTAA GGACACCACA 
GCGAAGGAAG TAGTCATTCA GGCCATCAGG GAGTTTGCTG TGACTGCCAC CCCGGATCAA 
TATTCGCTGT GCGAGGTCTC GGTCACACCT GAGGGTGTCA TCAAACAAAG ACGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGATAGAATA CAATTGAGTG GAAGGTACTA CTTGAAAAAC 
AACATGGAGA CAGAAACTCT TTGTTCAGAT GAAGATGCTC AGGAGCTGCT GAGGGAGAGT 
CAAATCTCCC TGTTGCAGCT CAGTACTGTT GAAGTTGCCA CACAGCTCTC TATGCGAAAC 
TTTGAACTCT TCCGTAACAT TGAACCCACC GAATATATAG ATGATTTATT TAAACTCAAA 
TCTAAAACCA GCTGTGCCAA CCTGAAGAAA TTTGAAGAAG TAATTAACCA AGAAACATTT 
TGGGTAGCAT CTGAAATTCT GAGAGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATTGCACT GCACTGCAGG GAATGCAAGA ATTTTAACTC CATGTTTGCA 
ATCATCAGTG GCCTAAACTT GGCCCCAGTG GCAAGACTAC GGACCACCTG GGAAAAACTT 
CCCAATAAAT ATGAAAAGCT GTTTCAAGAT CTCCAGGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ATCGCAATGT CCTCAATAGT CAAAACCTGC AGCCTCCCAT AATCCCCCTA 
TTCCCAGTTA TCAAGAAGGA TCTCACATTC CTTCATGAAG GAAATGACTC AAAAGTAGAT 
GGGTTAGTCA ATTTTGAGAA GCTAAGGATG ATCGCAAAAG AAATTCGTCA TGTTGGCCGA 
ATGGCTTCAG TTAACATGGA CCCTGCCCTC ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG 
AGTTTGGGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119398.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974873.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe40189
Clone ID Related Accession (Same CDS sequence)	XM_014119398.2 , XM_014119398.1
Accession Version	XM_014119398.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTTTTAGAGC CTTCTGAAAT GATTGTGGTG GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGT 
GAAAGACAAA CAATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
CGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAACTACAC 
CTTACTGATA GTCTCCACCC ACAGGTGACG CACGTTTCTT CTAGCCATTC AGGATGTAGT 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAAAGTGAG 
GCTGGTGATA TGGACCTGAG TGGGTTGCCA GAAACAGCAG TGGATTCTGA GGACGATGAT 
GATGAAGAAG ACATTGAGAG GGCATCAGAT CCTCTGATGA GTAGAGATAT TGTGAGAGAC 
TGTCTAGAGA AGGATCCAAT TGACAGGACA GATGATGACA TTGAACAACT CTTAGAATTT 
ATGCACCAGT TGCCTGCTTT TGCCAATATG ACAATGTCAG TGAGACGAGA ACTCTGTGCC 
GTGATGGTGT TTGCAGTGGT GGAAAGAGCT GGGACCATAG TGTTAAATGA TGGTGAAGAG 
CTGGATTCCT GGTCAGTGAT TCTGAATGGT TCTGTGGAAG TAACTTATCC AGATGGAAAA 
GCAGAAATAT TATGTATGGG AAATAGTTTT GGTGTCTCTC CTACCATGGA CAAAGAATAC 
ATGAAAGGAG TAATGAGAAC AAAAGTGGAT GACTGCCAGT TTGTCTGTAT AGCCCAGCAA 
GATTACTGCC GTATTCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA GGAAGAAGGA 
GAAATTGTTA TGGTAAAAGA ACACCGAGAA CTTGATAGAA CTGGAACAAG AAAGGGACAT 
ATTGTCATCA AGGGCACCTC AGAAAGATTA ACAATGCATT TGGTGGAAGA GCATTCGGTG 
GTGGATCCAA CATTCATAGA GGACTTCCTG TTGACCTATA GGACTTTTCT TTCTAGCCCA 
ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACACGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AACCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGATTGAT GACGTTAACA 
AAACCATCTC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GTGGCTCAGA GAAGGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGGT AGCAAAGCAA CTGAAGCAGG CTTGAAACGA 
GGGGACCAGA TATTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT GTCAAAAGCC 
ATGGAAATTC TTAGGAATAA CACACACTTA TCAATCACTG TGAAAACCAA CTTATTCGTA 
TTTAAAGAAC TTCTAACAAG ATTGTCAGAA GAGAAAAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTTGC TGTAGATGTA 
GAACAGGTGA TAGGACTTGA AAAAGTAAAT AAAAAAAGTA AAGCCAATAC TGTTGGAGGA 
AGGAACAAGC TAAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TTGGGATTGG TCAGTCTCAA GATGACAGTA TAGTGGGATT AAGACAGACG 
AAGCACATCC CAACTGCACT GCCCGTCAGT GGAACCTTGT CATCCAGTAA TCCTGATTTA 
TTACAGTCAC ATCATCGCAT TCTGGACTTC AGTACTACTC CTGACTTGCC AGATCAAGTG 
TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC CGATACATCA TGATCAGTAA GGACACCACA 
GCGAAGGAAG TAGTCATTCA GGCCATCAGG GAGTTTGCTG TGACTGCCAC CCCGGATCAA 
TATTCGCTGT GCGAGGTCTC GGTCACACCT GAGGGTGTCA TCAAACAAAG ACGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGATAGAATA CAATTGAGTG GAAGGTACTA CTTGAAAAAC 
AACATGGAGA CAGAAACTCT TTGTTCAGAT GAAGATGCTC AGGAGCTGCT GAGGGAGAGT 
CAAATCTCCC TGTTGCAGCT CAGTACTGTT GAAGTTGCCA CACAGCTCTC TATGCGAAAC 
TTTGAACTCT TCCGTAACAT TGAACCCACC GAATATATAG ATGATTTATT TAAACTCAAA 
TCTAAAACCA GCTGTGCCAA CCTGAAGAAA TTTGAAGAAG TAATTAACCA AGAAACATTT 
TGGGTAGCAT CTGAAATTCT GAGAGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATTGCACT GCACTGCAGG GAATGCAAGA ATTTTAACTC CATGTTTGCA 
ATCATCAGTG GCCTAAACTT GGCCCCAGTG GCAAGACTAC GGACCACCTG GGAAAAACTT 
CCCAATAAAT ATGAAAAGCT GTTTCAAGAT CTCCAGGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ATCGCAATGT CCTCAATAGT CAAAACCTGC AGCCTCCCAT AATCCCCCTA 
TTCCCAGTTA TCAAGAAGGA TCTCACATTC CTTCATGAAG GAAATGACTC AAAAGTAGAT 
GGGTTAGTCA ATTTTGAGAA GCTAAGGATG ATCGCAAAAG AAATTCGTCA TGTTGGCCGA 
ATGGCTTCAG TTAACATGGA CCCTGCCCTC ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG 
AGTTTGGGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974873.1
CDS	139..4971
Translation	MKTSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119398.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119398.1

ORF Insert Sequence:

ATGAAAACTA GTTTTGGCAA GCGTTCTGCA GGAAGCTTTA GGCGTGGCTG TGAATGCATT 
GTTTTAGAGC CTTCTGAAAT GATTGTGGTG GACTATATGG ATGAAAATGA AGAATATTTT 
CAGCGTCAAG CTTCTCATCG ACAGTCTCGA AGGAGATTTA GAAAAATCAA CCAGAAAGGT 
GAAAGACAAA CAATTATTGA CACTGTGGAT CCTTATCCTG TGGGCAAACC ACCTTTGCCT 
CGAGGCTATC ATACGGAATG CACTAAATCT CAGCTTCCTG CAGATTTCAC AAAACTACAC 
CTTACTGATA GTCTCCACCC ACAGGTGACG CACGTTTCTT CTAGCCATTC AGGATGTAGT 
ATCACTAGTG ATTCTGGAAG CAGCAGTCTT TCTGATATCT ACCAGGCCAC AGAAAGTGAG 
GCTGGTGATA TGGACCTGAG TGGGTTGCCA GAAACAGCAG TGGATTCTGA GGACGATGAT 
GATGAAGAAG ACATTGAGAG GGCATCAGAT CCTCTGATGA GTAGAGATAT TGTGAGAGAC 
TGTCTAGAGA AGGATCCAAT TGACAGGACA GATGATGACA TTGAACAACT CTTAGAATTT 
ATGCACCAGT TGCCTGCTTT TGCCAATATG ACAATGTCAG TGAGACGAGA ACTCTGTGCC 
GTGATGGTGT TTGCAGTGGT GGAAAGAGCT GGGACCATAG TGTTAAATGA TGGTGAAGAG 
CTGGATTCCT GGTCAGTGAT TCTGAATGGT TCTGTGGAAG TAACTTATCC AGATGGAAAA 
GCAGAAATAT TATGTATGGG AAATAGTTTT GGTGTCTCTC CTACCATGGA CAAAGAATAC 
ATGAAAGGAG TAATGAGAAC AAAAGTGGAT GACTGCCAGT TTGTCTGTAT AGCCCAGCAA 
GATTACTGCC GTATTCTCAA CCAAGTAGAA AAGAACATGC AAAAAGTTGA GGAAGAAGGA 
GAAATTGTTA TGGTAAAAGA ACACCGAGAA CTTGATAGAA CTGGAACAAG AAAGGGACAT 
ATTGTCATCA AGGGCACCTC AGAAAGATTA ACAATGCATT TGGTGGAAGA GCATTCGGTG 
GTGGATCCAA CATTCATAGA GGACTTCCTG TTGACCTATA GGACTTTTCT TTCTAGCCCA 
ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG TTTAATGACC CGAGCCTCAG GGATAAGGTT 
ACACGGGTAG TATTATTGTG GGTGAATAAT CACTTCAATG ACTTTGAAGG AGATCCTGCA 
ATGACTCGAT TTCTAGAAGA ATTTGAAAAT AACCTGGAAA GAGAGAAAAT GGGTGGACAT 
CTAAGGCTGT TAAATATTGC ATGTGCAGCT AAAGCAAAAA GAAGATTGAT GACGTTAACA 
AAACCATCTC GAGAAGCTCC TTTGCCTTTT ATCTTACTTG GTGGCTCAGA GAAGGGATTT 
GGAATCTTTG TTGACAGTGT AGATTCAGGT AGCAAAGCAA CTGAAGCAGG CTTGAAACGA 
GGGGACCAGA TATTAGAAGT AAATGGTCAA AACTTTGAAA ATATTCAGCT GTCAAAAGCC 
ATGGAAATTC TTAGGAATAA CACACACTTA TCAATCACTG TGAAAACCAA CTTATTCGTA 
TTTAAAGAAC TTCTAACAAG ATTGTCAGAA GAGAAAAGAA ATGGTGCCCC TCACCTCCCT 
AAAATTGGAG ACATCAAAAA GGCCAGTCGC TACTCCATTC CAGATCTTGC TGTAGATGTA 
GAACAGGTGA TAGGACTTGA AAAAGTAAAT AAAAAAAGTA AAGCCAATAC TGTTGGAGGA 
AGGAACAAGC TAAAAAAGAT ACTCGACAAG ACTCGGATCA GTATCTTGCC ACAGAAACCA 
TATAATGATA TTGGGATTGG TCAGTCTCAA GATGACAGTA TAGTGGGATT AAGACAGACG 
AAGCACATCC CAACTGCACT GCCCGTCAGT GGAACCTTGT CATCCAGTAA TCCTGATTTA 
TTACAGTCAC ATCATCGCAT TCTGGACTTC AGTACTACTC CTGACTTGCC AGATCAAGTG 
TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC CGATACATCA TGATCAGTAA GGACACCACA 
GCGAAGGAAG TAGTCATTCA GGCCATCAGG GAGTTTGCTG TGACTGCCAC CCCGGATCAA 
TATTCGCTGT GCGAGGTCTC GGTCACACCT GAGGGTGTCA TCAAACAAAG ACGGCTTCCA 
GATCAGCTTT CCAAACTTGC TGATAGAATA CAATTGAGTG GAAGGTACTA CTTGAAAAAC 
AACATGGAGA CAGAAACTCT TTGTTCAGAT GAAGATGCTC AGGAGCTGCT GAGGGAGAGT 
CAAATCTCCC TGTTGCAGCT CAGTACTGTT GAAGTTGCCA CACAGCTCTC TATGCGAAAC 
TTTGAACTCT TCCGTAACAT TGAACCCACC GAATATATAG ATGATTTATT TAAACTCAAA 
TCTAAAACCA GCTGTGCCAA CCTGAAGAAA TTTGAAGAAG TAATTAACCA AGAAACATTT 
TGGGTAGCAT CTGAAATTCT GAGAGAGACA AACCAGCTGA AGAGGATGAA GATCATTAAG 
CATTTCATCA AGATTGCACT GCACTGCAGG GAATGCAAGA ATTTTAACTC CATGTTTGCA 
ATCATCAGTG GCCTAAACTT GGCCCCAGTG GCAAGACTAC GGACCACCTG GGAAAAACTT 
CCCAATAAAT ATGAAAAGCT GTTTCAAGAT CTCCAGGACC TGTTTGATCC TTCCAGAAAC 
ATGGCAAAAT ATCGCAATGT CCTCAATAGT CAAAACCTGC AGCCTCCCAT AATCCCCCTA 
TTCCCAGTTA TCAAGAAGGA TCTCACATTC CTTCATGAAG GAAATGACTC AAAAGTAGAT 
GGGTTAGTCA ATTTTGAGAA GCTAAGGATG ATCGCAAAAG AAATTCGTCA TGTTGGCCGA 
ATGGCTTCAG TTAACATGGA CCCTGCCCTC ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG 
AGTTTGGGGT CTCTCAGCCA GGGTAGTACA AATGCAACAG TGCTAGACGT TGCTCAGACA 
GGTGGTCATA AAAAGCGGGT ACGTCGTAGT TCCTTTCTCA ATGCCAAAAA GCTTTATGAA 
GATGCCCAAA TGGCTCGAAA AGTGAAGCAG TACCTGTCCA ATTTGGAGCT AGAAATGGAT 
GAGGAGAGTC TTCAGACATT ATCACTGCAG TGTGAGCCAG CAACCAACAC ATTGCCTAAG 
AATCCTGGTG ACAAAAAGCC TGTCAAGTCT GAGACGTCCC CAGTGGCTCC AAGGGCAGGG 
TCACAACAGA AAGCACAAGC CCAGCCGCCG CCCCCGCAGC CACAGCCCCA ACATAAGATC 
AACCAAGGCC TTCAGGTTCC TGCTGTATCC CTTTATCCTT CACGAAAGAA AGTACCTGTA 
AAGGATCTCC CACCTTTTGG CATAAACTCT CCACAAGCTT TAAAGAAAAT TCTTTCTTTG 
TCTGAAGAAG GAAGTCTGGA GCGTCATAAA AAACAAGCAG AAGATACTAT ATCAAATGCA 
TCCTCACAGC TTTCTTCTCC TCCCACTTCT CCACAAAGTT CTCCAAGGAA AGGTGGCAGT 
GGCAATCAGC TGAGATCTTT TGGCTCCGGG CAGTTGGACT TAACCAGTTC CTCCTCTTCT 
CTTGGAAGTG AGAACAGTAA CAAGAATAAC AATGCACCAC GGACCTATGG GATAGGCTAC 
ACCTTGGCTC CCAGTGGTAC TGTGGATAAT TTTTCAGATT CTGGTCACAG TGAAATTTCT 
TCACGATCCA GTATTGTCAG CAATTCTTCC TTTGACTCCG TGCCAGTCTC GCTGCATGAG 
GAGAGGCGGC AGAGGCATTC CGTCAGCATC GTGGAAACAA ACCTCGGCGT GGGCAGGATG 
GAAAGGCGGA CTATGATGGA ACCAGATCAG TACAGCTTAG GGTCCTATGC ACCAATGGCT 
GAGAGTCGAG GCTTATATGC TACAGCTACG GTAATTTCTT CTCCAAGCAC AGAGGAACTT 
TCCCAAGATC AGGGGGATCG GGCTTCACTC GATGCTGCCG ACAGTGGCCG TGGGAGCTGG 
ACGTCGTGCT CAAGCGGTTC ACATGATAAC ATACAGACCA TCCAGCACCA GAGAAGCTGG 
GAAACACTTC CATTTGGGCA TACTCACTTT GACTATTCAG GGGATCCTGC AGGTTTATGG 
GCCTCAAGCA GCCATATGGA CCAAATAATG TTTTCCGATC ACAGCACAAA GTATAACAGG 
CAAAATCAAA GTAGAGAGAG TCTTGAACAA GCTCAGTCCC GGGCAAGCTG GGCATCTTCC 
ACAGGGTACT GGGGAGAAGA CTCAGAAGGT GACACAGGCA CAATCAAGCG AAGGGGTGGA 
AAAGATGTTT CCATTGAAGC TGAAAGCAGT AGCGTTACCT CTGTGACCAC AGAAGAAACC 
AAACCTGTTC CCATGCCTGC CCACGTGGCT GTGACATCGA GCACTGCAAA GGGACTTATT 
GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC CCGCCCACCC CTCCAGGCTA CATTGGAATC 
CCCATCACTG ACTTTCCCGA AGGACATTCC CATCCAGCCA GGAAGCCTCC AGACTACAAT 
GTGGCACTTC AGAGATCTCG GATGGTCGCC CGACCCACTG ACACAGCCGC GCCTTCGCCC 
ATACAGCAGC CGCACGGGCA TCCGGCGAGC GGAAGGCCGG TGAACAAACC ACAGTGGCAT 
AAACCCAATG AGTGTGACCC GCGCCTCGCC CCCTATCAGT CTCAAGGGTT TTCCACCGAG 
GAGGATGAAG ATGAACAAGT ATCTGCTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119399.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974874.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe40190
Clone ID Related Accession (Same CDS sequence)	XM_014119399.1 , XM_014119399.2
Accession Version	XM_014119399.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTCGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTAC ACCTTACTGA TAGTCTCCAC 
CCACAGGTGA CGCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGTG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCT GAGGACGATG ATGATGAAGA AGACATTGAG 
AGGGCATCAG ATCCTCTGAT GAGTAGAGAT ATTGTGAGAG ACTGTCTAGA GAAGGATCCA 
ATTGACAGGA CAGATGATGA CATTGAACAA CTCTTAGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGACGA GAACTCTGTG CCGTGATGGT GTTTGCAGTG 
GTGGAAAGAG CTGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGATTC CTGGTCAGTG 
ATTCTGAATG GTTCTGTGGA AGTAACTTAT CCAGATGGAA AAGCAGAAAT ATTATGTATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTAATGAGA 
ACAAAAGTGG ATGACTGCCA GTTTGTCTGT ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAAGAAG GAGAAATTGT TATGGTAAAA 
GAACACCGAG AACTTGATAG AACTGGAACA AGAAAGGGAC ATATTGTCAT CAAGGGCACC 
TCAGAAAGAT TAACAATGCA TTTGGTGGAA GAGCATTCGG TGGTGGATCC AACATTCATA 
GAGGACTTCC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAACCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC TCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGTGGCTCA GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GAGGGGACCA GATATTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTGTCAAAAG CCATGGAAAT TCTTAGGAAT 
AACACACACT TATCAATCAC TGTGAAAACC AACTTATTCG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTAA ATAAAAAAAG TAAAGCCAAT ACTGTTGGAG GAAGGAACAA GCTAAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG TATAGTGGGA TTAAGACAGA CGAAGCACAT CCCAACTGCA 
CTGCCCGTCA GTGGAACCTT GTCATCCAGT AATCCTGATT TATTACAGTC ACATCATCGC 
ATTCTGGACT TCAGTACTAC TCCTGACTTG CCAGATCAAG TGTTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGATACAT CATGATCAGT AAGGACACCA CAGCGAAGGA AGTAGTCATT 
CAGGCCATCA GGGAGTTTGC TGTGACTGCC ACCCCGGATC AATATTCGCT GTGCGAGGTC 
TCGGTCACAC CTGAGGGTGT CATCAAACAA AGACGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGATAGAA TACAATTGAG TGGAAGGTAC TACTTGAAAA ACAACATGGA GACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGCTG CTGAGGGAGA GTCAAATCTC CCTGTTGCAG 
CTCAGTACTG TTGAAGTTGC CACACAGCTC TCTATGCGAA ACTTTGAACT CTTCCGTAAC 
ATTGAACCCA CCGAATATAT AGATGATTTA TTTAAACTCA AATCTAAAAC CAGCTGTGCC 
AACCTGAAGA AATTTGAAGA AGTAATTAAC CAAGAAACAT TTTGGGTAGC ATCTGAAATT 
CTGAGAGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCA 
CTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC 
TTGGCCCCAG TGGCAAGACT ACGGACCACC TGGGAAAAAC TTCCCAATAA ATATGAAAAG 
CTGTTTCAAG ATCTCCAGGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGCAAT 
GTCCTCAATA GTCAAAACCT GCAGCCTCCC ATAATCCCCC TATTCCCAGT TATCAAGAAG 
GATCTCACAT TCCTTCATGA AGGAAATGAC TCAAAAGTAG ATGGGTTAGT CAATTTTGAG 
AAGCTAAGGA TGATCGCAAA AGAAATTCGT CATGTTGGCC GAATGGCTTC AGTTAACATG 
GACCCTGCCC TCATGTTCAG AACTCGGAAG AAGAAGTGGA GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAC GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTGTC CAATTTGGAG CTAGAAATGG ATGAGGAGAG TCTTCAGACA 
TTATCACTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAGT CTGAGACGTC CCCAGTGGCT CCAAGGGCAG GGTCACAACA GAAAGCACAA 
GCCCAGCCGC CGCCCCCGCA GCCACAGCCC CAACATAAGA TCAACCAAGG CCTTCAGGTT 
CCTGCTGTAT CCCTTTATCC TTCACGAAAG AAAGTACCTG TAAAGGATCT CCCACCTTTT 
GGCATAAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGTCTG 
GAGCGTCATA AAAAACAAGC AGAAGATACT ATATCAAATG CATCCTCACA GCTTTCTTCT 
CCTCCCACTT CTCCACAAAG TTCTCCAAGG AAAGGTGGCA GTGGCAATCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA CTTAACCAGT TCCTCCTCTT CTCTTGGAAG TGAGAACAGT 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACCTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTC 
AGCAATTCTT CCTTTGACTC CGTGCCAGTC TCGCTGCATG AGGAGAGGCG GCAGAGGCAT 
TCCGTCAGCA TCGTGGAAAC AAACCTCGGC GTGGGCAGGA TGGAAAGGCG GACTATGATG 
GAACCAGATC AGTACAGCTT AGGGTCCTAT GCACCAATGG CTGAGAGTCG AGGCTTATAT 
GCTACAGCTA CGGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAAGA TCAGGGGGAT 
CGGGCTTCAC TCGATGCTGC CGACAGTGGC CGTGGGAGCT GGACGTCGTG CTCAAGCGGT 
TCACATGATA ACATACAGAC CATCCAGCAC CAGAGAAGCT GGGAAACACT TCCATTTGGG 
CATACTCACT TTGACTATTC AGGGGATCCT GCAGGTTTAT GGGCCTCAAG CAGCCATATG 
GACCAAATAA TGTTTTCCGA TCACAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGTCTTGAAC AAGCTCAGTC CCGGGCAAGC TGGGCATCTT CCACAGGGTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATCAAG CGAAGGGGTG GAAAAGATGT TTCCATTGAA 
GCTGAAAGCA GTAGCGTTAC CTCTGTGACC ACAGAAGAAA CCAAACCTGT TCCCATGCCT 
GCCCACGTGG CTGTGACATC GAGCACTGCA AAGGGACTTA TTGTGCGGAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCAGGC TACATTGGAA TCCCCATCAC TGACTTTCCC 
GAAGGACATT CCCATCCAGC CAGGAAGCCT CCAGACTACA ATGTGGCACT TCAGAGATCT 
CGGATGGTCG CCCGACCCAC TGACACAGCC GCGCCTTCGC CCATACAGCA GCCGCACGGG 
CATCCGGCGA GCGGAAGGCC GGTGAACAAA CCACAGTGGC ATAAACCCAA TGAGTGTGAC 
CCGCGCCTCG CCCCCTATCA GTCTCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTATCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974874.1
CDS	232..4986
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119399.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119399.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTCGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTAC ACCTTACTGA TAGTCTCCAC 
CCACAGGTGA CGCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGTG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCT GAGGACGATG ATGATGAAGA AGACATTGAG 
AGGGCATCAG ATCCTCTGAT GAGTAGAGAT ATTGTGAGAG ACTGTCTAGA GAAGGATCCA 
ATTGACAGGA CAGATGATGA CATTGAACAA CTCTTAGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGACGA GAACTCTGTG CCGTGATGGT GTTTGCAGTG 
GTGGAAAGAG CTGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGATTC CTGGTCAGTG 
ATTCTGAATG GTTCTGTGGA AGTAACTTAT CCAGATGGAA AAGCAGAAAT ATTATGTATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTAATGAGA 
ACAAAAGTGG ATGACTGCCA GTTTGTCTGT ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAAGAAG GAGAAATTGT TATGGTAAAA 
GAACACCGAG AACTTGATAG AACTGGAACA AGAAAGGGAC ATATTGTCAT CAAGGGCACC 
TCAGAAAGAT TAACAATGCA TTTGGTGGAA GAGCATTCGG TGGTGGATCC AACATTCATA 
GAGGACTTCC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAACCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC TCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGTGGCTCA GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GAGGGGACCA GATATTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTGTCAAAAG CCATGGAAAT TCTTAGGAAT 
AACACACACT TATCAATCAC TGTGAAAACC AACTTATTCG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTAA ATAAAAAAAG TAAAGCCAAT ACTGTTGGAG GAAGGAACAA GCTAAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG TATAGTGGGA TTAAGACAGA CGAAGCACAT CCCAACTGCA 
CTGCCCGTCA GTGGAACCTT GTCATCCAGT AATCCTGATT TATTACAGTC ACATCATCGC 
ATTCTGGACT TCAGTACTAC TCCTGACTTG CCAGATCAAG TGTTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGATACAT CATGATCAGT AAGGACACCA CAGCGAAGGA AGTAGTCATT 
CAGGCCATCA GGGAGTTTGC TGTGACTGCC ACCCCGGATC AATATTCGCT GTGCGAGGTC 
TCGGTCACAC CTGAGGGTGT CATCAAACAA AGACGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGATAGAA TACAATTGAG TGGAAGGTAC TACTTGAAAA ACAACATGGA GACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGCTG CTGAGGGAGA GTCAAATCTC CCTGTTGCAG 
CTCAGTACTG TTGAAGTTGC CACACAGCTC TCTATGCGAA ACTTTGAACT CTTCCGTAAC 
ATTGAACCCA CCGAATATAT AGATGATTTA TTTAAACTCA AATCTAAAAC CAGCTGTGCC 
AACCTGAAGA AATTTGAAGA AGTAATTAAC CAAGAAACAT TTTGGGTAGC ATCTGAAATT 
CTGAGAGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCA 
CTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC 
TTGGCCCCAG TGGCAAGACT ACGGACCACC TGGGAAAAAC TTCCCAATAA ATATGAAAAG 
CTGTTTCAAG ATCTCCAGGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGCAAT 
GTCCTCAATA GTCAAAACCT GCAGCCTCCC ATAATCCCCC TATTCCCAGT TATCAAGAAG 
GATCTCACAT TCCTTCATGA AGGAAATGAC TCAAAAGTAG ATGGGTTAGT CAATTTTGAG 
AAGCTAAGGA TGATCGCAAA AGAAATTCGT CATGTTGGCC GAATGGCTTC AGTTAACATG 
GACCCTGCCC TCATGTTCAG AACTCGGAAG AAGAAGTGGA GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAC GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTGTC CAATTTGGAG CTAGAAATGG ATGAGGAGAG TCTTCAGACA 
TTATCACTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAGT CTGAGACGTC CCCAGTGGCT CCAAGGGCAG GGTCACAACA GAAAGCACAA 
GCCCAGCCGC CGCCCCCGCA GCCACAGCCC CAACATAAGA TCAACCAAGG CCTTCAGGTT 
CCTGCTGTAT CCCTTTATCC TTCACGAAAG AAAGTACCTG TAAAGGATCT CCCACCTTTT 
GGCATAAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGTCTG 
GAGCGTCATA AAAAACAAGC AGAAGATACT ATATCAAATG CATCCTCACA GCTTTCTTCT 
CCTCCCACTT CTCCACAAAG TTCTCCAAGG AAAGGTGGCA GTGGCAATCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA CTTAACCAGT TCCTCCTCTT CTCTTGGAAG TGAGAACAGT 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACCTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTC 
AGCAATTCTT CCTTTGACTC CGTGCCAGTC TCGCTGCATG AGGAGAGGCG GCAGAGGCAT 
TCCGTCAGCA TCGTGGAAAC AAACCTCGGC GTGGGCAGGA TGGAAAGGCG GACTATGATG 
GAACCAGATC AGTACAGCTT AGGGTCCTAT GCACCAATGG CTGAGAGTCG AGGCTTATAT 
GCTACAGCTA CGGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAAGA TCAGGGGGAT 
CGGGCTTCAC TCGATGCTGC CGACAGTGGC CGTGGGAGCT GGACGTCGTG CTCAAGCGGT 
TCACATGATA ACATACAGAC CATCCAGCAC CAGAGAAGCT GGGAAACACT TCCATTTGGG 
CATACTCACT TTGACTATTC AGGGGATCCT GCAGGTTTAT GGGCCTCAAG CAGCCATATG 
GACCAAATAA TGTTTTCCGA TCACAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGTCTTGAAC AAGCTCAGTC CCGGGCAAGC TGGGCATCTT CCACAGGGTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATCAAG CGAAGGGGTG GAAAAGATGT TTCCATTGAA 
GCTGAAAGCA GTAGCGTTAC CTCTGTGACC ACAGAAGAAA CCAAACCTGT TCCCATGCCT 
GCCCACGTGG CTGTGACATC GAGCACTGCA AAGGGACTTA TTGTGCGGAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCAGGC TACATTGGAA TCCCCATCAC TGACTTTCCC 
GAAGGACATT CCCATCCAGC CAGGAAGCCT CCAGACTACA ATGTGGCACT TCAGAGATCT 
CGGATGGTCG CCCGACCCAC TGACACAGCC GCGCCTTCGC CCATACAGCA GCCGCACGGG 
CATCCGGCGA GCGGAAGGCC GGTGAACAAA CCACAGTGGC ATAAACCCAA TGAGTGTGAC 
CCGCGCCTCG CCCCCTATCA GTCTCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTATCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119399.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974874.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

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CloneID	OCe40190
Clone ID Related Accession (Same CDS sequence)	XM_014119399.1 , XM_014119399.2
Accession Version	XM_014119399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTCGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTAC ACCTTACTGA TAGTCTCCAC 
CCACAGGTGA CGCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGTG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCT GAGGACGATG ATGATGAAGA AGACATTGAG 
AGGGCATCAG ATCCTCTGAT GAGTAGAGAT ATTGTGAGAG ACTGTCTAGA GAAGGATCCA 
ATTGACAGGA CAGATGATGA CATTGAACAA CTCTTAGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGACGA GAACTCTGTG CCGTGATGGT GTTTGCAGTG 
GTGGAAAGAG CTGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGATTC CTGGTCAGTG 
ATTCTGAATG GTTCTGTGGA AGTAACTTAT CCAGATGGAA AAGCAGAAAT ATTATGTATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTAATGAGA 
ACAAAAGTGG ATGACTGCCA GTTTGTCTGT ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAAGAAG GAGAAATTGT TATGGTAAAA 
GAACACCGAG AACTTGATAG AACTGGAACA AGAAAGGGAC ATATTGTCAT CAAGGGCACC 
TCAGAAAGAT TAACAATGCA TTTGGTGGAA GAGCATTCGG TGGTGGATCC AACATTCATA 
GAGGACTTCC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAACCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC TCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGTGGCTCA GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GAGGGGACCA GATATTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTGTCAAAAG CCATGGAAAT TCTTAGGAAT 
AACACACACT TATCAATCAC TGTGAAAACC AACTTATTCG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTAA ATAAAAAAAG TAAAGCCAAT ACTGTTGGAG GAAGGAACAA GCTAAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG TATAGTGGGA TTAAGACAGA CGAAGCACAT CCCAACTGCA 
CTGCCCGTCA GTGGAACCTT GTCATCCAGT AATCCTGATT TATTACAGTC ACATCATCGC 
ATTCTGGACT TCAGTACTAC TCCTGACTTG CCAGATCAAG TGTTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGATACAT CATGATCAGT AAGGACACCA CAGCGAAGGA AGTAGTCATT 
CAGGCCATCA GGGAGTTTGC TGTGACTGCC ACCCCGGATC AATATTCGCT GTGCGAGGTC 
TCGGTCACAC CTGAGGGTGT CATCAAACAA AGACGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGATAGAA TACAATTGAG TGGAAGGTAC TACTTGAAAA ACAACATGGA GACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGCTG CTGAGGGAGA GTCAAATCTC CCTGTTGCAG 
CTCAGTACTG TTGAAGTTGC CACACAGCTC TCTATGCGAA ACTTTGAACT CTTCCGTAAC 
ATTGAACCCA CCGAATATAT AGATGATTTA TTTAAACTCA AATCTAAAAC CAGCTGTGCC 
AACCTGAAGA AATTTGAAGA AGTAATTAAC CAAGAAACAT TTTGGGTAGC ATCTGAAATT 
CTGAGAGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCA 
CTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC 
TTGGCCCCAG TGGCAAGACT ACGGACCACC TGGGAAAAAC TTCCCAATAA ATATGAAAAG 
CTGTTTCAAG ATCTCCAGGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGCAAT 
GTCCTCAATA GTCAAAACCT GCAGCCTCCC ATAATCCCCC TATTCCCAGT TATCAAGAAG 
GATCTCACAT TCCTTCATGA AGGAAATGAC TCAAAAGTAG ATGGGTTAGT CAATTTTGAG 
AAGCTAAGGA TGATCGCAAA AGAAATTCGT CATGTTGGCC GAATGGCTTC AGTTAACATG 
GACCCTGCCC TCATGTTCAG AACTCGGAAG AAGAAGTGGA GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAC GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTGTC CAATTTGGAG CTAGAAATGG ATGAGGAGAG TCTTCAGACA 
TTATCACTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAGT CTGAGACGTC CCCAGTGGCT CCAAGGGCAG GGTCACAACA GAAAGCACAA 
GCCCAGCCGC CGCCCCCGCA GCCACAGCCC CAACATAAGA TCAACCAAGG CCTTCAGGTT 
CCTGCTGTAT CCCTTTATCC TTCACGAAAG AAAGTACCTG TAAAGGATCT CCCACCTTTT 
GGCATAAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGTCTG 
GAGCGTCATA AAAAACAAGC AGAAGATACT ATATCAAATG CATCCTCACA GCTTTCTTCT 
CCTCCCACTT CTCCACAAAG TTCTCCAAGG AAAGGTGGCA GTGGCAATCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA CTTAACCAGT TCCTCCTCTT CTCTTGGAAG TGAGAACAGT 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACCTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTC 
AGCAATTCTT CCTTTGACTC CGTGCCAGTC TCGCTGCATG AGGAGAGGCG GCAGAGGCAT 
TCCGTCAGCA TCGTGGAAAC AAACCTCGGC GTGGGCAGGA TGGAAAGGCG GACTATGATG 
GAACCAGATC AGTACAGCTT AGGGTCCTAT GCACCAATGG CTGAGAGTCG AGGCTTATAT 
GCTACAGCTA CGGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAAGA TCAGGGGGAT 
CGGGCTTCAC TCGATGCTGC CGACAGTGGC CGTGGGAGCT GGACGTCGTG CTCAAGCGGT 
TCACATGATA ACATACAGAC CATCCAGCAC CAGAGAAGCT GGGAAACACT TCCATTTGGG 
CATACTCACT TTGACTATTC AGGGGATCCT GCAGGTTTAT GGGCCTCAAG CAGCCATATG 
GACCAAATAA TGTTTTCCGA TCACAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGTCTTGAAC AAGCTCAGTC CCGGGCAAGC TGGGCATCTT CCACAGGGTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATCAAG CGAAGGGGTG GAAAAGATGT TTCCATTGAA 
GCTGAAAGCA GTAGCGTTAC CTCTGTGACC ACAGAAGAAA CCAAACCTGT TCCCATGCCT 
GCCCACGTGG CTGTGACATC GAGCACTGCA AAGGGACTTA TTGTGCGGAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCAGGC TACATTGGAA TCCCCATCAC TGACTTTCCC 
GAAGGACATT CCCATCCAGC CAGGAAGCCT CCAGACTACA ATGTGGCACT TCAGAGATCT 
CGGATGGTCG CCCGACCCAC TGACACAGCC GCGCCTTCGC CCATACAGCA GCCGCACGGG 
CATCCGGCGA GCGGAAGGCC GGTGAACAAA CCACAGTGGC ATAAACCCAA TGAGTGTGAC 
CCGCGCCTCG CCCCCTATCA GTCTCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTATCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974874.1
CDS	213..4967
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119399.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119399.2

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGTCA AGCTTCTCAT 
CGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC TGTGGGCAAA CCACCTTTGC CTCGAGGCTA TCATACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTAC ACCTTACTGA TAGTCTCCAC 
CCACAGGTGA CGCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGA 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGTG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCT GAGGACGATG ATGATGAAGA AGACATTGAG 
AGGGCATCAG ATCCTCTGAT GAGTAGAGAT ATTGTGAGAG ACTGTCTAGA GAAGGATCCA 
ATTGACAGGA CAGATGATGA CATTGAACAA CTCTTAGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGACGA GAACTCTGTG CCGTGATGGT GTTTGCAGTG 
GTGGAAAGAG CTGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGATTC CTGGTCAGTG 
ATTCTGAATG GTTCTGTGGA AGTAACTTAT CCAGATGGAA AAGCAGAAAT ATTATGTATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTAATGAGA 
ACAAAAGTGG ATGACTGCCA GTTTGTCTGT ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAAGAAG GAGAAATTGT TATGGTAAAA 
GAACACCGAG AACTTGATAG AACTGGAACA AGAAAGGGAC ATATTGTCAT CAAGGGCACC 
TCAGAAAGAT TAACAATGCA TTTGGTGGAA GAGCATTCGG TGGTGGATCC AACATTCATA 
GAGGACTTCC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTGAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTCTAGAA 
GAATTTGAAA ATAACCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTAAATATT 
GCATGTGCAG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC TCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGTGGCTCA GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GAGGGGACCA GATATTAGAA 
GTAAATGGTC AAAACTTTGA AAATATTCAG CTGTCAAAAG CCATGGAAAT TCTTAGGAAT 
AACACACACT TATCAATCAC TGTGAAAACC AACTTATTCG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCTCACCTCC CTAAAATTGG AGACATCAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTAA ATAAAAAAAG TAAAGCCAAT ACTGTTGGAG GAAGGAACAA GCTAAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATATAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG TATAGTGGGA TTAAGACAGA CGAAGCACAT CCCAACTGCA 
CTGCCCGTCA GTGGAACCTT GTCATCCAGT AATCCTGATT TATTACAGTC ACATCATCGC 
ATTCTGGACT TCAGTACTAC TCCTGACTTG CCAGATCAAG TGTTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGATACAT CATGATCAGT AAGGACACCA CAGCGAAGGA AGTAGTCATT 
CAGGCCATCA GGGAGTTTGC TGTGACTGCC ACCCCGGATC AATATTCGCT GTGCGAGGTC 
TCGGTCACAC CTGAGGGTGT CATCAAACAA AGACGGCTTC CAGATCAGCT TTCCAAACTT 
GCTGATAGAA TACAATTGAG TGGAAGGTAC TACTTGAAAA ACAACATGGA GACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGCTG CTGAGGGAGA GTCAAATCTC CCTGTTGCAG 
CTCAGTACTG TTGAAGTTGC CACACAGCTC TCTATGCGAA ACTTTGAACT CTTCCGTAAC 
ATTGAACCCA CCGAATATAT AGATGATTTA TTTAAACTCA AATCTAAAAC CAGCTGTGCC 
AACCTGAAGA AATTTGAAGA AGTAATTAAC CAAGAAACAT TTTGGGTAGC ATCTGAAATT 
CTGAGAGAGA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCA 
CTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC 
TTGGCCCCAG TGGCAAGACT ACGGACCACC TGGGAAAAAC TTCCCAATAA ATATGAAAAG 
CTGTTTCAAG ATCTCCAGGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGCAAT 
GTCCTCAATA GTCAAAACCT GCAGCCTCCC ATAATCCCCC TATTCCCAGT TATCAAGAAG 
GATCTCACAT TCCTTCATGA AGGAAATGAC TCAAAAGTAG ATGGGTTAGT CAATTTTGAG 
AAGCTAAGGA TGATCGCAAA AGAAATTCGT CATGTTGGCC GAATGGCTTC AGTTAACATG 
GACCCTGCCC TCATGTTCAG AACTCGGAAG AAGAAGTGGA GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAC GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTGTC CAATTTGGAG CTAGAAATGG ATGAGGAGAG TCTTCAGACA 
TTATCACTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAGT CTGAGACGTC CCCAGTGGCT CCAAGGGCAG GGTCACAACA GAAAGCACAA 
GCCCAGCCGC CGCCCCCGCA GCCACAGCCC CAACATAAGA TCAACCAAGG CCTTCAGGTT 
CCTGCTGTAT CCCTTTATCC TTCACGAAAG AAAGTACCTG TAAAGGATCT CCCACCTTTT 
GGCATAAACT CTCCACAAGC TTTAAAGAAA ATTCTTTCTT TGTCTGAAGA AGGAAGTCTG 
GAGCGTCATA AAAAACAAGC AGAAGATACT ATATCAAATG CATCCTCACA GCTTTCTTCT 
CCTCCCACTT CTCCACAAAG TTCTCCAAGG AAAGGTGGCA GTGGCAATCA GCTGAGATCT 
TTTGGCTCCG GGCAGTTGGA CTTAACCAGT TCCTCCTCTT CTCTTGGAAG TGAGAACAGT 
AACAAGAATA ACAATGCACC ACGGACCTAT GGGATAGGCT ACACCTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTC 
AGCAATTCTT CCTTTGACTC CGTGCCAGTC TCGCTGCATG AGGAGAGGCG GCAGAGGCAT 
TCCGTCAGCA TCGTGGAAAC AAACCTCGGC GTGGGCAGGA TGGAAAGGCG GACTATGATG 
GAACCAGATC AGTACAGCTT AGGGTCCTAT GCACCAATGG CTGAGAGTCG AGGCTTATAT 
GCTACAGCTA CGGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAAGA TCAGGGGGAT 
CGGGCTTCAC TCGATGCTGC CGACAGTGGC CGTGGGAGCT GGACGTCGTG CTCAAGCGGT 
TCACATGATA ACATACAGAC CATCCAGCAC CAGAGAAGCT GGGAAACACT TCCATTTGGG 
CATACTCACT TTGACTATTC AGGGGATCCT GCAGGTTTAT GGGCCTCAAG CAGCCATATG 
GACCAAATAA TGTTTTCCGA TCACAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGTCTTGAAC AAGCTCAGTC CCGGGCAAGC TGGGCATCTT CCACAGGGTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATCAAG CGAAGGGGTG GAAAAGATGT TTCCATTGAA 
GCTGAAAGCA GTAGCGTTAC CTCTGTGACC ACAGAAGAAA CCAAACCTGT TCCCATGCCT 
GCCCACGTGG CTGTGACATC GAGCACTGCA AAGGGACTTA TTGTGCGGAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCAGGC TACATTGGAA TCCCCATCAC TGACTTTCCC 
GAAGGACATT CCCATCCAGC CAGGAAGCCT CCAGACTACA ATGTGGCACT TCAGAGATCT 
CGGATGGTCG CCCGACCCAC TGACACAGCC GCGCCTTCGC CCATACAGCA GCCGCACGGG 
CATCCGGCGA GCGGAAGGCC GGTGAACAAA CCACAGTGGC ATAAACCCAA TGAGTGTGAC 
CCGCGCCTCG CCCCCTATCA GTCTCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTATCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014119400.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974875.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X10, mRNA.

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CloneID	OCe40191
Clone ID Related Accession (Same CDS sequence)	XM_014119400.1
Accession Version	XM_014119400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTTCCT 
GCAGATTTCA CAAAACTACA CCTTACTGAT AGTCTCCACC CACAGGTGAC GCACGTTTCT 
TCTAGCCATT CAGGATGTAG TATCACTAGT GATTCTGGAA GCAGCAGTCT TTCTGATATC 
TACCAGGCCA CAGAAAGTGA GGCTGGTGAT ATGGACCTGA GTGGGTTGCC AGAAACAGCA 
GTGGATTCTG AGGACGATGA TGATGAAGAA GACATTGAGA GGGCATCAGA TCCTCTGATG 
AGTAGAGATA TTGTGAGAGA CTGTCTAGAG AAGGATCCAA TTGACAGGAC AGATGATGAC 
ATTGAACAAC TCTTAGAATT TATGCACCAG TTGCCTGCTT TTGCCAATAT GACAATGTCA 
GTGAGACGAG AACTCTGTGC CGTGATGGTG TTTGCAGTGG TGGAAAGAGC TGGGACCATA 
GTGTTAAATG ATGGTGAAGA GCTGGATTCC TGGTCAGTGA TTCTGAATGG TTCTGTGGAA 
GTAACTTATC CAGATGGAAA AGCAGAAATA TTATGTATGG GAAATAGTTT TGGTGTCTCT 
CCTACCATGG ACAAAGAATA CATGAAAGGA GTAATGAGAA CAAAAGTGGA TGACTGCCAG 
TTTGTCTGTA TAGCCCAGCA AGATTACTGC CGTATTCTCA ACCAAGTAGA AAAGAACATG 
CAAAAAGTTG AGGAAGAAGG AGAAATTGTT ATGGTAAAAG AACACCGAGA ACTTGATAGA 
ACTGGAACAA GAAAGGGACA TATTGTCATC AAGGGCACCT CAGAAAGATT AACAATGCAT 
TTGGTGGAAG AGCATTCGGT GGTGGATCCA ACATTCATAG AGGACTTCCT GTTGACCTAT 
AGGACTTTTC TTTCTAGCCC AATGGAAGTG GGCAAAAAGT TATTGGAGTG GTTTAATGAC 
CCGAGCCTCA GGGATAAGGT TACACGGGTA GTATTATTGT GGGTGAATAA TCACTTCAAT 
GACTTTGAAG GAGATCCTGC AATGACTCGA TTTCTAGAAG AATTTGAAAA TAACCTGGAA 
AGAGAGAAAA TGGGTGGACA TCTAAGGCTG TTAAATATTG CATGTGCAGC TAAAGCAAAA 
AGAAGATTGA TGACGTTAAC AAAACCATCT CGAGAAGCTC CTTTGCCTTT TATCTTACTT 
GGTGGCTCAG AGAAGGGATT TGGAATCTTT GTTGACAGTG TAGATTCAGG TAGCAAAGCA 
ACTGAAGCAG GCTTGAAACG AGGGGACCAG ATATTAGAAG TAAATGGTCA AAACTTTGAA 
AATATTCAGC TGTCAAAAGC CATGGAAATT CTTAGGAATA ACACACACTT ATCAATCACT 
GTGAAAACCA ACTTATTCGT ATTTAAAGAA CTTCTAACAA GATTGTCAGA AGAGAAAAGA 
AATGGTGCCC CTCACCTCCC TAAAATTGGA GACATCAAAA AGGCCAGTCG CTACTCCATT 
CCAGATCTTG CTGTAGATGT AGAACAGGTG ATAGGACTTG AAAAAGTAAA TAAAAAAAGT 
AAAGCCAATA CTGTTGGAGG AAGGAACAAG CTAAAAAAGA TACTCGACAA GACTCGGATC 
AGTATCTTGC CACAGAAACC ATATAATGAT ATTGGGATTG GTCAGTCTCA AGATGACAGT 
ATAGTGGGAT TAAGACAGAC GAAGCACATC CCAACTGCAC TGCCCGTCAG TGGAACCTTG 
TCATCCAGTA ATCCTGATTT ATTACAGTCA CATCATCGCA TTCTGGACTT CAGTACTACT 
CCTGACTTGC CAGATCAAGT GTTAAGGGTT TTTAAGGCTG ATCAGCAAAG CCGATACATC 
ATGATCAGTA AGGACACCAC AGCGAAGGAA GTAGTCATTC AGGCCATCAG GGAGTTTGCT 
GTGACTGCCA CCCCGGATCA ATATTCGCTG TGCGAGGTCT CGGTCACACC TGAGGGTGTC 
ATCAAACAAA GACGGCTTCC AGATCAGCTT TCCAAACTTG CTGATAGAAT ACAATTGAGT 
GGAAGGTACT ACTTGAAAAA CAACATGGAG ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAGGAGCTGC TGAGGGAGAG TCAAATCTCC CTGTTGCAGC TCAGTACTGT TGAAGTTGCC 
ACACAGCTCT CTATGCGAAA CTTTGAACTC TTCCGTAACA TTGAACCCAC CGAATATATA 
GATGATTTAT TTAAACTCAA ATCTAAAACC AGCTGTGCCA ACCTGAAGAA ATTTGAAGAA 
GTAATTAACC AAGAAACATT TTGGGTAGCA TCTGAAATTC TGAGAGAGAC AAACCAGCTG 
AAGAGGATGA AGATCATTAA GCATTTCATC AAGATTGCAC TGCACTGCAG GGAATGCAAG 
AATTTTAACT CCATGTTTGC AATCATCAGT GGCCTAAACT TGGCCCCAGT GGCAAGACTA 
CGGACCACCT GGGAAAAACT TCCCAATAAA TATGAAAAGC TGTTTCAAGA TCTCCAGGAC 
CTGTTTGATC CTTCCAGAAA CATGGCAAAA TATCGCAATG TCCTCAATAG TCAAAACCTG 
CAGCCTCCCA TAATCCCCCT ATTCCCAGTT ATCAAGAAGG ATCTCACATT CCTTCATGAA 
GGAAATGACT CAAAAGTAGA TGGGTTAGTC AATTTTGAGA AGCTAAGGAT GATCGCAAAA 
GAAATTCGTC ATGTTGGCCG AATGGCTTCA GTTAACATGG ACCCTGCCCT CATGTTCAGA 
ACTCGGAAGA AGAAGTGGAG GAGTTTGGGG TCTCTCAGCC AGGGTAGTAC AAATGCAACA 
GTGCTAGACG TTGCTCAGAC AGGTGGTCAT AAAAAGCGGG TACGTCGTAG TTCCTTTCTC 
AATGCCAAAA AGCTTTATGA AGATGCCCAA ATGGCTCGAA AAGTGAAGCA GTACCTGTCC 
AATTTGGAGC TAGAAATGGA TGAGGAGAGT CTTCAGACAT TATCACTGCA GTGTGAGCCA 
GCAACCAACA CATTGCCTAA GAATCCTGGT GACAAAAAGC CTGTCAAGTC TGAGACGTCC 
CCAGTGGCTC CAAGGGCAGG GTCACAACAG AAAGCACAAG CCCAGCCGCC GCCCCCGCAG 
CCACAGCCCC AACATAAGAT CAACCAAGGC CTTCAGGTTC CTGCTGTATC CCTTTATCCT 
TCACGAAAGA AAGTACCTGT AAAGGATCTC CCACCTTTTG GCATAAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGTCTGG AGCGTCATAA AAAACAAGCA 
GAAGATACTA TATCAAATGC ATCCTCACAG CTTTCTTCTC CTCCCACTTC TCCACAAAGT 
TCTCCAAGGA AAGGTGGCAG TGGCAATCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAC 
TTAACCAGTT CCTCCTCTTC TCTTGGAAGT GAGAACAGTA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACCTTGGCT CCCAGTGGTA CTGTGGATAA TTTTTCAGAT 
TCTGGTCACA GTGAAATTTC TTCACGATCC AGTATTGTCA GCAATTCTTC CTTTGACTCC 
GTGCCAGTCT CGCTGCATGA GGAGAGGCGG CAGAGGCATT CCGTCAGCAT CGTGGAAACA 
AACCTCGGCG TGGGCAGGAT GGAAAGGCGG ACTATGATGG AACCAGATCA GTACAGCTTA 
GGGTCCTATG CACCAATGGC TGAGAGTCGA GGCTTATATG CTACAGCTAC GGTAATTTCT 
TCTCCAAGCA CAGAGGAACT TTCCCAAGAT CAGGGGGATC GGGCTTCACT CGATGCTGCC 
GACAGTGGCC GTGGGAGCTG GACGTCGTGC TCAAGCGGTT CACATGATAA CATACAGACC 
ATCCAGCACC AGAGAAGCTG GGAAACACTT CCATTTGGGC ATACTCACTT TGACTATTCA 
GGGGATCCTG CAGGTTTATG GGCCTCAAGC AGCCATATGG ACCAAATAAT GTTTTCCGAT 
CACAGCACAA AGTATAACAG GCAAAATCAA AGTAGAGAGA GTCTTGAACA AGCTCAGTCC 
CGGGCAAGCT GGGCATCTTC CACAGGGTAC TGGGGAGAAG ACTCAGAAGG TGACACAGGC 
ACAATCAAGC GAAGGGGTGG AAAAGATGTT TCCATTGAAG CTGAAAGCAG TAGCGTTACC 
TCTGTGACCA CAGAAGAAAC CAAACCTGTT CCCATGCCTG CCCACGTGGC TGTGACATCG 
AGCACTGCAA AGGGACTTAT TGTGCGGAAG GAGGGCAGGT ATCGAGAGCC CCCGCCCACC 
CCTCCAGGCT ACATTGGAAT CCCCATCACT GACTTTCCCG AAGGACATTC CCATCCAGCC 
AGGAAGCCTC CAGACTACAA TGTGGCACTT CAGAGATCTC GGATGGTCGC CCGACCCACT 
GACACAGCCG CGCCTTCGCC CATACAGCAG CCGCACGGGC ATCCGGCGAG CGGAAGGCCG 
GTGAACAAAC CACAGTGGCA TAAACCCAAT GAGTGTGACC CGCGCCTCGC CCCCTATCAG 
TCTCAAGGGT TTTCCACCGA GGAGGATGAA GATGAACAAG TATCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974875.1
CDS	137..4750
Translation	MAFLVRCYANCLQPWSSKLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014119400.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119400.1

ORF Insert Sequence:

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTTCCT 
GCAGATTTCA CAAAACTACA CCTTACTGAT AGTCTCCACC CACAGGTGAC GCACGTTTCT 
TCTAGCCATT CAGGATGTAG TATCACTAGT GATTCTGGAA GCAGCAGTCT TTCTGATATC 
TACCAGGCCA CAGAAAGTGA GGCTGGTGAT ATGGACCTGA GTGGGTTGCC AGAAACAGCA 
GTGGATTCTG AGGACGATGA TGATGAAGAA GACATTGAGA GGGCATCAGA TCCTCTGATG 
AGTAGAGATA TTGTGAGAGA CTGTCTAGAG AAGGATCCAA TTGACAGGAC AGATGATGAC 
ATTGAACAAC TCTTAGAATT TATGCACCAG TTGCCTGCTT TTGCCAATAT GACAATGTCA 
GTGAGACGAG AACTCTGTGC CGTGATGGTG TTTGCAGTGG TGGAAAGAGC TGGGACCATA 
GTGTTAAATG ATGGTGAAGA GCTGGATTCC TGGTCAGTGA TTCTGAATGG TTCTGTGGAA 
GTAACTTATC CAGATGGAAA AGCAGAAATA TTATGTATGG GAAATAGTTT TGGTGTCTCT 
CCTACCATGG ACAAAGAATA CATGAAAGGA GTAATGAGAA CAAAAGTGGA TGACTGCCAG 
TTTGTCTGTA TAGCCCAGCA AGATTACTGC CGTATTCTCA ACCAAGTAGA AAAGAACATG 
CAAAAAGTTG AGGAAGAAGG AGAAATTGTT ATGGTAAAAG AACACCGAGA ACTTGATAGA 
ACTGGAACAA GAAAGGGACA TATTGTCATC AAGGGCACCT CAGAAAGATT AACAATGCAT 
TTGGTGGAAG AGCATTCGGT GGTGGATCCA ACATTCATAG AGGACTTCCT GTTGACCTAT 
AGGACTTTTC TTTCTAGCCC AATGGAAGTG GGCAAAAAGT TATTGGAGTG GTTTAATGAC 
CCGAGCCTCA GGGATAAGGT TACACGGGTA GTATTATTGT GGGTGAATAA TCACTTCAAT 
GACTTTGAAG GAGATCCTGC AATGACTCGA TTTCTAGAAG AATTTGAAAA TAACCTGGAA 
AGAGAGAAAA TGGGTGGACA TCTAAGGCTG TTAAATATTG CATGTGCAGC TAAAGCAAAA 
AGAAGATTGA TGACGTTAAC AAAACCATCT CGAGAAGCTC CTTTGCCTTT TATCTTACTT 
GGTGGCTCAG AGAAGGGATT TGGAATCTTT GTTGACAGTG TAGATTCAGG TAGCAAAGCA 
ACTGAAGCAG GCTTGAAACG AGGGGACCAG ATATTAGAAG TAAATGGTCA AAACTTTGAA 
AATATTCAGC TGTCAAAAGC CATGGAAATT CTTAGGAATA ACACACACTT ATCAATCACT 
GTGAAAACCA ACTTATTCGT ATTTAAAGAA CTTCTAACAA GATTGTCAGA AGAGAAAAGA 
AATGGTGCCC CTCACCTCCC TAAAATTGGA GACATCAAAA AGGCCAGTCG CTACTCCATT 
CCAGATCTTG CTGTAGATGT AGAACAGGTG ATAGGACTTG AAAAAGTAAA TAAAAAAAGT 
AAAGCCAATA CTGTTGGAGG AAGGAACAAG CTAAAAAAGA TACTCGACAA GACTCGGATC 
AGTATCTTGC CACAGAAACC ATATAATGAT ATTGGGATTG GTCAGTCTCA AGATGACAGT 
ATAGTGGGAT TAAGACAGAC GAAGCACATC CCAACTGCAC TGCCCGTCAG TGGAACCTTG 
TCATCCAGTA ATCCTGATTT ATTACAGTCA CATCATCGCA TTCTGGACTT CAGTACTACT 
CCTGACTTGC CAGATCAAGT GTTAAGGGTT TTTAAGGCTG ATCAGCAAAG CCGATACATC 
ATGATCAGTA AGGACACCAC AGCGAAGGAA GTAGTCATTC AGGCCATCAG GGAGTTTGCT 
GTGACTGCCA CCCCGGATCA ATATTCGCTG TGCGAGGTCT CGGTCACACC TGAGGGTGTC 
ATCAAACAAA GACGGCTTCC AGATCAGCTT TCCAAACTTG CTGATAGAAT ACAATTGAGT 
GGAAGGTACT ACTTGAAAAA CAACATGGAG ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAGGAGCTGC TGAGGGAGAG TCAAATCTCC CTGTTGCAGC TCAGTACTGT TGAAGTTGCC 
ACACAGCTCT CTATGCGAAA CTTTGAACTC TTCCGTAACA TTGAACCCAC CGAATATATA 
GATGATTTAT TTAAACTCAA ATCTAAAACC AGCTGTGCCA ACCTGAAGAA ATTTGAAGAA 
GTAATTAACC AAGAAACATT TTGGGTAGCA TCTGAAATTC TGAGAGAGAC AAACCAGCTG 
AAGAGGATGA AGATCATTAA GCATTTCATC AAGATTGCAC TGCACTGCAG GGAATGCAAG 
AATTTTAACT CCATGTTTGC AATCATCAGT GGCCTAAACT TGGCCCCAGT GGCAAGACTA 
CGGACCACCT GGGAAAAACT TCCCAATAAA TATGAAAAGC TGTTTCAAGA TCTCCAGGAC 
CTGTTTGATC CTTCCAGAAA CATGGCAAAA TATCGCAATG TCCTCAATAG TCAAAACCTG 
CAGCCTCCCA TAATCCCCCT ATTCCCAGTT ATCAAGAAGG ATCTCACATT CCTTCATGAA 
GGAAATGACT CAAAAGTAGA TGGGTTAGTC AATTTTGAGA AGCTAAGGAT GATCGCAAAA 
GAAATTCGTC ATGTTGGCCG AATGGCTTCA GTTAACATGG ACCCTGCCCT CATGTTCAGA 
ACTCGGAAGA AGAAGTGGAG GAGTTTGGGG TCTCTCAGCC AGGGTAGTAC AAATGCAACA 
GTGCTAGACG TTGCTCAGAC AGGTGGTCAT AAAAAGCGGG TACGTCGTAG TTCCTTTCTC 
AATGCCAAAA AGCTTTATGA AGATGCCCAA ATGGCTCGAA AAGTGAAGCA GTACCTGTCC 
AATTTGGAGC TAGAAATGGA TGAGGAGAGT CTTCAGACAT TATCACTGCA GTGTGAGCCA 
GCAACCAACA CATTGCCTAA GAATCCTGGT GACAAAAAGC CTGTCAAGTC TGAGACGTCC 
CCAGTGGCTC CAAGGGCAGG GTCACAACAG AAAGCACAAG CCCAGCCGCC GCCCCCGCAG 
CCACAGCCCC AACATAAGAT CAACCAAGGC CTTCAGGTTC CTGCTGTATC CCTTTATCCT 
TCACGAAAGA AAGTACCTGT AAAGGATCTC CCACCTTTTG GCATAAACTC TCCACAAGCT 
TTAAAGAAAA TTCTTTCTTT GTCTGAAGAA GGAAGTCTGG AGCGTCATAA AAAACAAGCA 
GAAGATACTA TATCAAATGC ATCCTCACAG CTTTCTTCTC CTCCCACTTC TCCACAAAGT 
TCTCCAAGGA AAGGTGGCAG TGGCAATCAG CTGAGATCTT TTGGCTCCGG GCAGTTGGAC 
TTAACCAGTT CCTCCTCTTC TCTTGGAAGT GAGAACAGTA ACAAGAATAA CAATGCACCA 
CGGACCTATG GGATAGGCTA CACCTTGGCT CCCAGTGGTA CTGTGGATAA TTTTTCAGAT 
TCTGGTCACA GTGAAATTTC TTCACGATCC AGTATTGTCA GCAATTCTTC CTTTGACTCC 
GTGCCAGTCT CGCTGCATGA GGAGAGGCGG CAGAGGCATT CCGTCAGCAT CGTGGAAACA 
AACCTCGGCG TGGGCAGGAT GGAAAGGCGG ACTATGATGG AACCAGATCA GTACAGCTTA 
GGGTCCTATG CACCAATGGC TGAGAGTCGA GGCTTATATG CTACAGCTAC GGTAATTTCT 
TCTCCAAGCA CAGAGGAACT TTCCCAAGAT CAGGGGGATC GGGCTTCACT CGATGCTGCC 
GACAGTGGCC GTGGGAGCTG GACGTCGTGC TCAAGCGGTT CACATGATAA CATACAGACC 
ATCCAGCACC AGAGAAGCTG GGAAACACTT CCATTTGGGC ATACTCACTT TGACTATTCA 
GGGGATCCTG CAGGTTTATG GGCCTCAAGC AGCCATATGG ACCAAATAAT GTTTTCCGAT 
CACAGCACAA AGTATAACAG GCAAAATCAA AGTAGAGAGA GTCTTGAACA AGCTCAGTCC 
CGGGCAAGCT GGGCATCTTC CACAGGGTAC TGGGGAGAAG ACTCAGAAGG TGACACAGGC 
ACAATCAAGC GAAGGGGTGG AAAAGATGTT TCCATTGAAG CTGAAAGCAG TAGCGTTACC 
TCTGTGACCA CAGAAGAAAC CAAACCTGTT CCCATGCCTG CCCACGTGGC TGTGACATCG 
AGCACTGCAA AGGGACTTAT TGTGCGGAAG GAGGGCAGGT ATCGAGAGCC CCCGCCCACC 
CCTCCAGGCT ACATTGGAAT CCCCATCACT GACTTTCCCG AAGGACATTC CCATCCAGCC 
AGGAAGCCTC CAGACTACAA TGTGGCACTT CAGAGATCTC GGATGGTCGC CCGACCCACT 
GACACAGCCG CGCCTTCGCC CATACAGCAG CCGCACGGGC ATCCGGCGAG CGGAAGGCCG 
GTGAACAAAC CACAGTGGCA TAAACCCAAT GAGTGTGACC CGCGCCTCGC CCCCTATCAG 
TCTCAAGGGT TTTCCACCGA GGAGGATGAA GATGAACAAG TATCTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005629386.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629443.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X9, mRNA.

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CloneID	OCe17766
Clone ID Related Accession (Same CDS sequence)	XM_005629386.2 , XM_005629386.3
Accession Version	XM_005629386.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTACACC TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCCTTT 
GACTCCGTGC CAGTCTCGCT GCATGAGGAG AGGCGGCAGA GGCATTCCGT CAGCATCGTG 
GAAACAAACC TCGGCGTGGG CAGGATGGAA AGGCGGACTA TGATGGAACC AGATCAGTAC 
AGCTTAGGGT CCTATGCACC AATGGCTGAG AGTCGAGGCT TATATGCTAC AGCTACGGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAAGATCAGG GGGATCGGGC TTCACTCGAT 
GCTGCCGACA GTGGCCGTGG GAGCTGGACG TCGTGCTCAA GCGGTTCACA TGATAACATA 
CAGACCATCC AGCACCAGAG AAGCTGGGAA ACACTTCCAT TTGGGCATAC TCACTTTGAC 
TATTCAGGGG ATCCTGCAGG TTTATGGGCC TCAAGCAGCC ATATGGACCA AATAATGTTT 
TCCGATCACA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGTCT TGAACAAGCT 
CAGTCCCGGG CAAGCTGGGC ATCTTCCACA GGGTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TCAAGCGAAG GGGTGGAAAA GATGTTTCCA TTGAAGCTGA AAGCAGTAGC 
GTTACCTCTG TGACCACAGA AGAAACCAAA CCTGTTCCCA TGCCTGCCCA CGTGGCTGTG 
ACATCGAGCA CTGCAAAGGG ACTTATTGTG CGGAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CAGGCTACAT TGGAATCCCC ATCACTGACT TTCCCGAAGG ACATTCCCAT 
CCAGCCAGGA AGCCTCCAGA CTACAATGTG GCACTTCAGA GATCTCGGAT GGTCGCCCGA 
CCCACTGACA CAGCCGCGCC TTCGCCCATA CAGCAGCCGC ACGGGCATCC GGCGAGCGGA 
AGGCCGGTGA ACAAACCACA GTGGCATAAA CCCAATGAGT GTGACCCGCG CCTCGCCCCC 
TATCAGTCTC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTATC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629443.1
CDS	351..4970
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005629386.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629386.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTACACC TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCCTTT 
GACTCCGTGC CAGTCTCGCT GCATGAGGAG AGGCGGCAGA GGCATTCCGT CAGCATCGTG 
GAAACAAACC TCGGCGTGGG CAGGATGGAA AGGCGGACTA TGATGGAACC AGATCAGTAC 
AGCTTAGGGT CCTATGCACC AATGGCTGAG AGTCGAGGCT TATATGCTAC AGCTACGGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAAGATCAGG GGGATCGGGC TTCACTCGAT 
GCTGCCGACA GTGGCCGTGG GAGCTGGACG TCGTGCTCAA GCGGTTCACA TGATAACATA 
CAGACCATCC AGCACCAGAG AAGCTGGGAA ACACTTCCAT TTGGGCATAC TCACTTTGAC 
TATTCAGGGG ATCCTGCAGG TTTATGGGCC TCAAGCAGCC ATATGGACCA AATAATGTTT 
TCCGATCACA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGTCT TGAACAAGCT 
CAGTCCCGGG CAAGCTGGGC ATCTTCCACA GGGTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TCAAGCGAAG GGGTGGAAAA GATGTTTCCA TTGAAGCTGA AAGCAGTAGC 
GTTACCTCTG TGACCACAGA AGAAACCAAA CCTGTTCCCA TGCCTGCCCA CGTGGCTGTG 
ACATCGAGCA CTGCAAAGGG ACTTATTGTG CGGAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CAGGCTACAT TGGAATCCCC ATCACTGACT TTCCCGAAGG ACATTCCCAT 
CCAGCCAGGA AGCCTCCAGA CTACAATGTG GCACTTCAGA GATCTCGGAT GGTCGCCCGA 
CCCACTGACA CAGCCGCGCC TTCGCCCATA CAGCAGCCGC ACGGGCATCC GGCGAGCGGA 
AGGCCGGTGA ACAAACCACA GTGGCATAAA CCCAATGAGT GTGACCCGCG CCTCGCCCCC 
TATCAGTCTC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTATC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005629386.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629443.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

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CloneID	OCe17766
Clone ID Related Accession (Same CDS sequence)	XM_005629386.2 , XM_005629386.3
Accession Version	XM_005629386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTACACC TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCCTTT 
GACTCCGTGC CAGTCTCGCT GCATGAGGAG AGGCGGCAGA GGCATTCCGT CAGCATCGTG 
GAAACAAACC TCGGCGTGGG CAGGATGGAA AGGCGGACTA TGATGGAACC AGATCAGTAC 
AGCTTAGGGT CCTATGCACC AATGGCTGAG AGTCGAGGCT TATATGCTAC AGCTACGGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAAGATCAGG GGGATCGGGC TTCACTCGAT 
GCTGCCGACA GTGGCCGTGG GAGCTGGACG TCGTGCTCAA GCGGTTCACA TGATAACATA 
CAGACCATCC AGCACCAGAG AAGCTGGGAA ACACTTCCAT TTGGGCATAC TCACTTTGAC 
TATTCAGGGG ATCCTGCAGG TTTATGGGCC TCAAGCAGCC ATATGGACCA AATAATGTTT 
TCCGATCACA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGTCT TGAACAAGCT 
CAGTCCCGGG CAAGCTGGGC ATCTTCCACA GGGTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TCAAGCGAAG GGGTGGAAAA GATGTTTCCA TTGAAGCTGA AAGCAGTAGC 
GTTACCTCTG TGACCACAGA AGAAACCAAA CCTGTTCCCA TGCCTGCCCA CGTGGCTGTG 
ACATCGAGCA CTGCAAAGGG ACTTATTGTG CGGAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CAGGCTACAT TGGAATCCCC ATCACTGACT TTCCCGAAGG ACATTCCCAT 
CCAGCCAGGA AGCCTCCAGA CTACAATGTG GCACTTCAGA GATCTCGGAT GGTCGCCCGA 
CCCACTGACA CAGCCGCGCC TTCGCCCATA CAGCAGCCGC ACGGGCATCC GGCGAGCGGA 
AGGCCGGTGA ACAAACCACA GTGGCATAAA CCCAATGAGT GTGACCCGCG CCTCGCCCCC 
TATCAGTCTC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTATC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629443.1
CDS	88..4707
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005629386.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629386.3

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTACACC TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCCTTT 
GACTCCGTGC CAGTCTCGCT GCATGAGGAG AGGCGGCAGA GGCATTCCGT CAGCATCGTG 
GAAACAAACC TCGGCGTGGG CAGGATGGAA AGGCGGACTA TGATGGAACC AGATCAGTAC 
AGCTTAGGGT CCTATGCACC AATGGCTGAG AGTCGAGGCT TATATGCTAC AGCTACGGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAAGATCAGG GGGATCGGGC TTCACTCGAT 
GCTGCCGACA GTGGCCGTGG GAGCTGGACG TCGTGCTCAA GCGGTTCACA TGATAACATA 
CAGACCATCC AGCACCAGAG AAGCTGGGAA ACACTTCCAT TTGGGCATAC TCACTTTGAC 
TATTCAGGGG ATCCTGCAGG TTTATGGGCC TCAAGCAGCC ATATGGACCA AATAATGTTT 
TCCGATCACA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGTCT TGAACAAGCT 
CAGTCCCGGG CAAGCTGGGC ATCTTCCACA GGGTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TCAAGCGAAG GGGTGGAAAA GATGTTTCCA TTGAAGCTGA AAGCAGTAGC 
GTTACCTCTG TGACCACAGA AGAAACCAAA CCTGTTCCCA TGCCTGCCCA CGTGGCTGTG 
ACATCGAGCA CTGCAAAGGG ACTTATTGTG CGGAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CAGGCTACAT TGGAATCCCC ATCACTGACT TTCCCGAAGG ACATTCCCAT 
CCAGCCAGGA AGCCTCCAGA CTACAATGTG GCACTTCAGA GATCTCGGAT GGTCGCCCGA 
CCCACTGACA CAGCCGCGCC TTCGCCCATA CAGCAGCCGC ACGGGCATCC GGCGAGCGGA 
AGGCCGGTGA ACAAACCACA GTGGCATAAA CCCAATGAGT GTGACCCGCG CCTCGCCCCC 
TATCAGTCTC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTATC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005629387.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629444.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe17767
Clone ID Related Accession (Same CDS sequence)	XM_005629387.2
Accession Version	XM_005629387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTG CAGATTTCAC AAAACTACAC CTTACTGATA GTCTCCACCC ACAGGTGACG 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGTGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCTGA GGACGATGAT GATGAAGAAG ACATTGAGAG GGCATCAGAT 
CCTCTGATGA GTAGAGATAT TGTGAGAGAC TGTCTAGAGA AGGATCCAAT TGACAGGACA 
GATGATGACA TTGAACAACT CTTAGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGACGAGA ACTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGATTCCT GGTCAGTGAT TCTGAATGGT 
TCTGTGGAAG TAACTTATCC AGATGGAAAA GCAGAAATAT TATGTATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TAATGAGAAC AAAAGTGGAT 
GACTGCCAGT TTGTCTGTAT AGCCCAGCAA GATTACTGCC GTATTCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA GGAAGAAGGA GAAATTGTTA TGGTAAAAGA ACACCGAGAA 
CTTGATAGAA CTGGAACAAG AAAGGGACAT ATTGTCATCA AGGGCACCTC AGAAAGATTA 
ACAATGCATT TGGTGGAAGA GCATTCGGTG GTGGATCCAA CATTCATAGA GGACTTCCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AACCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCTC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GTGGCTCAGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGA GGGGACCAGA TATTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTAGGAATAA CACACACTTA 
TCAATCACTG TGAAAACCAA CTTATTCGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTAAAT 
AAAAAAAGTA AAGCCAATAC TGTTGGAGGA AGGAACAAGC TAAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGTA TAGTGGGATT AAGACAGACG AAGCACATCC CAACTGCACT GCCCGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATTTA TTACAGTCAC ATCATCGCAT TCTGGACTTC 
AGTACTACTC CTGACTTGCC AGATCAAGTG TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACCACA GCGAAGGAAG TAGTCATTCA GGCCATCAGG 
GAGTTTGCTG TGACTGCCAC CCCGGATCAA TATTCGCTGT GCGAGGTCTC GGTCACACCT 
GAGGGTGTCA TCAAACAAAG ACGGCTTCCA GATCAGCTTT CCAAACTTGC TGATAGAATA 
CAATTGAGTG GAAGGTACTA CTTGAAAAAC AACATGGAGA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGCTGCT GAGGGAGAGT CAAATCTCCC TGTTGCAGCT CAGTACTGTT 
GAAGTTGCCA CACAGCTCTC TATGCGAAAC TTTGAACTCT TCCGTAACAT TGAACCCACC 
GAATATATAG ATGATTTATT TAAACTCAAA TCTAAAACCA GCTGTGCCAA CCTGAAGAAA 
TTTGAAGAAG TAATTAACCA AGAAACATTT TGGGTAGCAT CTGAAATTCT GAGAGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCACT GCACTGCAGG 
GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACTT GGCCCCAGTG 
GCAAGACTAC GGACCACCTG GGAAAAACTT CCCAATAAAT ATGAAAAGCT GTTTCAAGAT 
CTCCAGGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGCAATGT CCTCAATAGT 
CAAAACCTGC AGCCTCCCAT AATCCCCCTA TTCCCAGTTA TCAAGAAGGA TCTCACATTC 
CTTCATGAAG GAAATGACTC AAAAGTAGAT GGGTTAGTCA ATTTTGAGAA GCTAAGGATG 
ATCGCAAAAG AAATTCGTCA TGTTGGCCGA ATGGCTTCAG TTAACATGGA CCCTGCCCTC 
ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGACGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTGTCCA ATTTGGAGCT AGAAATGGAT GAGGAGAGTC TTCAGACATT ATCACTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAGTCT 
GAGACGTCCC CAGTGGCTCC AAGGGCAGGG TCACAACAGA AAGCACAAGC CCAGCCGCCG 
CCCCCGCAGC CACAGCCCCA ACATAAGATC AACCAAGGCC TTCAGGTTCC TGCTGTATCC 
CTTTATCCTT CACGAAAGAA AGTACCTGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAGAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTCTGGA GCGTCATAAA 
AAACAAGCAG AAGATACTAT ATCAAATGCA TCCTCACAGC TTTCTTCTCC TCCCACTTCT 
CCACAAAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAC ACCTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTCAG CAATTCTTCC 
TTTGACTCCG TGCCAGTCTC GCTGCATGAG GAGAGGCGGC AGAGGCATTC CGTCAGCATC 
GTGGAAACAA ACCTCGGCGT GGGCAGGATG GAAAGGCGGA CTATGATGGA ACCAGATCAG 
TACAGCTTAG GGTCCTATGC ACCAATGGCT GAGAGTCGAG GCTTATATGC TACAGCTACG 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAAGATC AGGGGGATCG GGCTTCACTC 
GATGCTGCCG ACAGTGGCCG TGGGAGCTGG ACGTCGTGCT CAAGCGGTTC ACATGATAAC 
ATACAGACCA TCCAGCACCA GAGAAGCTGG GAAACACTTC CATTTGGGCA TACTCACTTT 
GACTATTCAG GGGATCCTGC AGGTTTATGG GCCTCAAGCA GCCATATGGA CCAAATAATG 
TTTTCCGATC ACAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG TCTTGAACAA 
GCTCAGTCCC GGGCAAGCTG GGCATCTTCC ACAGGGTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATCAAGCG AAGGGGTGGA AAAGATGTTT CCATTGAAGC TGAAAGCAGT 
AGCGTTACCT CTGTGACCAC AGAAGAAACC AAACCTGTTC CCATGCCTGC CCACGTGGCT 
GTGACATCGA GCACTGCAAA GGGACTTATT GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCAGGCTA CATTGGAATC CCCATCACTG ACTTTCCCGA AGGACATTCC 
CATCCAGCCA GGAAGCCTCC AGACTACAAT GTGGCACTTC AGAGATCTCG GATGGTCGCC 
CGACCCACTG ACACAGCCGC GCCTTCGCCC ATACAGCAGC CGCACGGGCA TCCGGCGAGC 
GGAAGGCCGG TGAACAAACC ACAGTGGCAT AAACCCAATG AGTGTGACCC GCGCCTCGCC 
CCCTATCAGT CTCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT ATCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629444.1
CDS	104..4666
Translation	MLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005629387.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629387.2

ORF Insert Sequence:

ATGCTTCCTG CAGATTTCAC AAAACTACAC CTTACTGATA GTCTCCACCC ACAGGTGACG 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGAAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGTGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCTGA GGACGATGAT GATGAAGAAG ACATTGAGAG GGCATCAGAT 
CCTCTGATGA GTAGAGATAT TGTGAGAGAC TGTCTAGAGA AGGATCCAAT TGACAGGACA 
GATGATGACA TTGAACAACT CTTAGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGACGAGA ACTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGATTCCT GGTCAGTGAT TCTGAATGGT 
TCTGTGGAAG TAACTTATCC AGATGGAAAA GCAGAAATAT TATGTATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TAATGAGAAC AAAAGTGGAT 
GACTGCCAGT TTGTCTGTAT AGCCCAGCAA GATTACTGCC GTATTCTCAA CCAAGTAGAA 
AAGAACATGC AAAAAGTTGA GGAAGAAGGA GAAATTGTTA TGGTAAAAGA ACACCGAGAA 
CTTGATAGAA CTGGAACAAG AAAGGGACAT ATTGTCATCA AGGGCACCTC AGAAAGATTA 
ACAATGCATT TGGTGGAAGA GCATTCGGTG GTGGATCCAA CATTCATAGA GGACTTCCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTGAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTCTAGAAGA ATTTGAAAAT 
AACCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TAAATATTGC ATGTGCAGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCTC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GTGGCTCAGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGA GGGGACCAGA TATTAGAAGT AAATGGTCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTAGGAATAA CACACACTTA 
TCAATCACTG TGAAAACCAA CTTATTCGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC TCACCTCCCT AAAATTGGAG ACATCAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTAAAT 
AAAAAAAGTA AAGCCAATAC TGTTGGAGGA AGGAACAAGC TAAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TATAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGTA TAGTGGGATT AAGACAGACG AAGCACATCC CAACTGCACT GCCCGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATTTA TTACAGTCAC ATCATCGCAT TCTGGACTTC 
AGTACTACTC CTGACTTGCC AGATCAAGTG TTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGATACATCA TGATCAGTAA GGACACCACA GCGAAGGAAG TAGTCATTCA GGCCATCAGG 
GAGTTTGCTG TGACTGCCAC CCCGGATCAA TATTCGCTGT GCGAGGTCTC GGTCACACCT 
GAGGGTGTCA TCAAACAAAG ACGGCTTCCA GATCAGCTTT CCAAACTTGC TGATAGAATA 
CAATTGAGTG GAAGGTACTA CTTGAAAAAC AACATGGAGA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGCTGCT GAGGGAGAGT CAAATCTCCC TGTTGCAGCT CAGTACTGTT 
GAAGTTGCCA CACAGCTCTC TATGCGAAAC TTTGAACTCT TCCGTAACAT TGAACCCACC 
GAATATATAG ATGATTTATT TAAACTCAAA TCTAAAACCA GCTGTGCCAA CCTGAAGAAA 
TTTGAAGAAG TAATTAACCA AGAAACATTT TGGGTAGCAT CTGAAATTCT GAGAGAGACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCACT GCACTGCAGG 
GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACTT GGCCCCAGTG 
GCAAGACTAC GGACCACCTG GGAAAAACTT CCCAATAAAT ATGAAAAGCT GTTTCAAGAT 
CTCCAGGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGCAATGT CCTCAATAGT 
CAAAACCTGC AGCCTCCCAT AATCCCCCTA TTCCCAGTTA TCAAGAAGGA TCTCACATTC 
CTTCATGAAG GAAATGACTC AAAAGTAGAT GGGTTAGTCA ATTTTGAGAA GCTAAGGATG 
ATCGCAAAAG AAATTCGTCA TGTTGGCCGA ATGGCTTCAG TTAACATGGA CCCTGCCCTC 
ATGTTCAGAA CTCGGAAGAA GAAGTGGAGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGACGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTGTCCA ATTTGGAGCT AGAAATGGAT GAGGAGAGTC TTCAGACATT ATCACTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAGTCT 
GAGACGTCCC CAGTGGCTCC AAGGGCAGGG TCACAACAGA AAGCACAAGC CCAGCCGCCG 
CCCCCGCAGC CACAGCCCCA ACATAAGATC AACCAAGGCC TTCAGGTTCC TGCTGTATCC 
CTTTATCCTT CACGAAAGAA AGTACCTGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAGAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTCTGGA GCGTCATAAA 
AAACAAGCAG AAGATACTAT ATCAAATGCA TCCTCACAGC TTTCTTCTCC TCCCACTTCT 
CCACAAAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAC ACCTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTCAG CAATTCTTCC 
TTTGACTCCG TGCCAGTCTC GCTGCATGAG GAGAGGCGGC AGAGGCATTC CGTCAGCATC 
GTGGAAACAA ACCTCGGCGT GGGCAGGATG GAAAGGCGGA CTATGATGGA ACCAGATCAG 
TACAGCTTAG GGTCCTATGC ACCAATGGCT GAGAGTCGAG GCTTATATGC TACAGCTACG 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAAGATC AGGGGGATCG GGCTTCACTC 
GATGCTGCCG ACAGTGGCCG TGGGAGCTGG ACGTCGTGCT CAAGCGGTTC ACATGATAAC 
ATACAGACCA TCCAGCACCA GAGAAGCTGG GAAACACTTC CATTTGGGCA TACTCACTTT 
GACTATTCAG GGGATCCTGC AGGTTTATGG GCCTCAAGCA GCCATATGGA CCAAATAATG 
TTTTCCGATC ACAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG TCTTGAACAA 
GCTCAGTCCC GGGCAAGCTG GGCATCTTCC ACAGGGTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATCAAGCG AAGGGGTGGA AAAGATGTTT CCATTGAAGC TGAAAGCAGT 
AGCGTTACCT CTGTGACCAC AGAAGAAACC AAACCTGTTC CCATGCCTGC CCACGTGGCT 
GTGACATCGA GCACTGCAAA GGGACTTATT GTGCGGAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCAGGCTA CATTGGAATC CCCATCACTG ACTTTCCCGA AGGACATTCC 
CATCCAGCCA GGAAGCCTCC AGACTACAAT GTGGCACTTC AGAGATCTCG GATGGTCGCC 
CGACCCACTG ACACAGCCGC GCCTTCGCCC ATACAGCAGC CGCACGGGCA TCCGGCGAGC 
GGAAGGCCGG TGAACAAACC ACAGTGGCAT AAACCCAATG AGTGTGACCC GCGCCTCGCC 
CCCTATCAGT CTCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT ATCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_003639497.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003639545.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X12, mRNA.

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CloneID	OCe17768
Clone ID Related Accession (Same CDS sequence)	XM_003639497.3 , XM_003639497.4
Accession Version	XM_003639497.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 2 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003639497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003639545.1
CDS	349..4845
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_003639497.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003639497.3

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_003639497.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003639545.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

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CloneID	OCe17768
Clone ID Related Accession (Same CDS sequence)	XM_003639497.3 , XM_003639497.4
Accession Version	XM_003639497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003639497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003639545.1
CDS	1..4497
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQAQPPPPQPQPQHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHEERRQRHSVSIVETNLGVGRMERRTMMEPDQYSLGSYAPMAESRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSVTSVTTEETKPVPMPAHVAVTSSTAKGLIVRKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARPTDTAAPSPIQQPHGHPASGRPVNKPQWHKPNECDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_003639497.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003639497.4

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACATTCA 
CTTCCTGCAG ATTTCACAAA ACTACACCTT ACTGATAGTC TCCACCCACA GGTGACGCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGAAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGTGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCTGAGGA CGATGATGAT GAAGAAGACA TTGAGAGGGC ATCAGATCCT 
CTGATGAGTA GAGATATTGT GAGAGACTGT CTAGAGAAGG ATCCAATTGA CAGGACAGAT 
GATGACATTG AACAACTCTT AGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GACGAGAACT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GATTCCTGGT CAGTGATTCT GAATGGTTCT 
GTGGAAGTAA CTTATCCAGA TGGAAAAGCA GAAATATTAT GTATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTAA TGAGAACAAA AGTGGATGAC 
TGCCAGTTTG TCTGTATAGC CCAGCAAGAT TACTGCCGTA TTCTCAACCA AGTAGAAAAG 
AACATGCAAA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAAGAACA CCGAGAACTT 
GATAGAACTG GAACAAGAAA GGGACATATT GTCATCAAGG GCACCTCAGA AAGATTAACA 
ATGCATTTGG TGGAAGAGCA TTCGGTGGTG GATCCAACAT TCATAGAGGA CTTCCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT GAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTC TAGAAGAATT TGAAAATAAC 
CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTAA ATATTGCATG TGCAGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCTCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGTG GCTCAGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGAGGG GACCAGATAT TAGAAGTAAA TGGTCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTA GGAATAACAC ACACTTATCA 
ATCACTGTGA AAACCAACTT ATTCGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCTCA CCTCCCTAAA ATTGGAGACA TCAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTAAATAAA 
AAAAGTAAAG CCAATACTGT TGGAGGAAGG AACAAGCTAA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAT AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGTATAG TGGGATTAAG ACAGACGAAG CACATCCCAA CTGCACTGCC CGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATTTATTA CAGTCACATC ATCGCATTCT GGACTTCAGT 
ACTACTCCTG ACTTGCCAGA TCAAGTGTTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGA 
TACATCATGA TCAGTAAGGA CACCACAGCG AAGGAAGTAG TCATTCAGGC CATCAGGGAG 
TTTGCTGTGA CTGCCACCCC GGATCAATAT TCGCTGTGCG AGGTCTCGGT CACACCTGAG 
GGTGTCATCA AACAAAGACG GCTTCCAGAT CAGCTTTCCA AACTTGCTGA TAGAATACAA 
TTGAGTGGAA GGTACTACTT GAAAAACAAC ATGGAGACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGCTGCTGAG GGAGAGTCAA ATCTCCCTGT TGCAGCTCAG TACTGTTGAA 
GTTGCCACAC AGCTCTCTAT GCGAAACTTT GAACTCTTCC GTAACATTGA ACCCACCGAA 
TATATAGATG ATTTATTTAA ACTCAAATCT AAAACCAGCT GTGCCAACCT GAAGAAATTT 
GAAGAAGTAA TTAACCAAGA AACATTTTGG GTAGCATCTG AAATTCTGAG AGAGACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCACTGCA CTGCAGGGAA 
TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACTTGGC CCCAGTGGCA 
AGACTACGGA CCACCTGGGA AAAACTTCCC AATAAATATG AAAAGCTGTT TCAAGATCTC 
CAGGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GCAATGTCCT CAATAGTCAA 
AACCTGCAGC CTCCCATAAT CCCCCTATTC CCAGTTATCA AGAAGGATCT CACATTCCTT 
CATGAAGGAA ATGACTCAAA AGTAGATGGG TTAGTCAATT TTGAGAAGCT AAGGATGATC 
GCAAAAGAAA TTCGTCATGT TGGCCGAATG GCTTCAGTTA ACATGGACCC TGCCCTCATG 
TTCAGAACTC GGAAGAAGAA GTGGAGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGACGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTGTCCAATT TGGAGCTAGA AATGGATGAG GAGAGTCTTC AGACATTATC ACTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAGTCTGAG 
ACGTCCCCAG TGGCTCCAAG GGCAGGGTCA CAACAGAAAG CACAAGCCCA GCCGCCGCCC 
CCGCAGCCAC AGCCCCAACA TAAGATCAAC CAAGGCCTTC AGGTTCCTGC TGTATCCCTT 
TATCCTTCAC GAAAGAAAGT ACCTGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AGAAAATTCT TTCTTTGTCT GAAGAAGGAA GTCTGGAGCG TCATAAAAAA 
CAAGCAGAAG ATACTATATC AAATGCATCC TCACAGCTTT CTTCTCCTCC CACTTCTCCA 
CAAAGTTCTC CAAGGAAAGG CTACACCTTG GCTCCCAGTG GTACTGTGGA TAATTTTTCA 
GATTCTGGTC ACAGTGAAAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCCTTTGAC 
TCCGTGCCAG TCTCGCTGCA TGAGGAGAGG CGGCAGAGGC ATTCCGTCAG CATCGTGGAA 
ACAAACCTCG GCGTGGGCAG GATGGAAAGG CGGACTATGA TGGAACCAGA TCAGTACAGC 
TTAGGGTCCT ATGCACCAAT GGCTGAGAGT CGAGGCTTAT ATGCTACAGC TACGGTAATT 
TCTTCTCCAA GCACAGAGGA ACTTTCCCAA GATCAGGGGG ATCGGGCTTC ACTCGATGCT 
GCCGACAGTG GCCGTGGGAG CTGGACGTCG TGCTCAAGCG GTTCACATGA TAACATACAG 
ACCATCCAGC ACCAGAGAAG CTGGGAAACA CTTCCATTTG GGCATACTCA CTTTGACTAT 
TCAGGGGATC CTGCAGGTTT ATGGGCCTCA AGCAGCCATA TGGACCAAAT AATGTTTTCC 
GATCACAGCA CAAAGTATAA CAGGCAAAAT CAAAGTAGAG AGAGTCTTGA ACAAGCTCAG 
TCCCGGGCAA GCTGGGCATC TTCCACAGGG TACTGGGGAG AAGACTCAGA AGGTGACACA 
GGCACAATCA AGCGAAGGGG TGGAAAAGAT GTTTCCATTG AAGCTGAAAG CAGTAGCGTT 
ACCTCTGTGA CCACAGAAGA AACCAAACCT GTTCCCATGC CTGCCCACGT GGCTGTGACA 
TCGAGCACTG CAAAGGGACT TATTGTGCGG AAGGAGGGCA GGTATCGAGA GCCCCCGCCC 
ACCCCTCCAG GCTACATTGG AATCCCCATC ACTGACTTTC CCGAAGGACA TTCCCATCCA 
GCCAGGAAGC CTCCAGACTA CAATGTGGCA CTTCAGAGAT CTCGGATGGT CGCCCGACCC 
ACTGACACAG CCGCGCCTTC GCCCATACAG CAGCCGCACG GGCATCCGGC GAGCGGAAGG 
CCGGTGAACA AACCACAGTG GCATAAACCC AATGAGTGTG ACCCGCGCCT CGCCCCCTAT 
CAGTCTCAAG GGTTTTCCAC CGAGGAGGAT GAAGATGAAC AAGTATCTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF2
Entrez Gene ID	100856359
Full Name	Rap guanine nucleotide exchange factor 2
Synonyms	CNrasGEF,NRAPGEP,PDZ-GEF1,PDZGEF1,RA-GEF-1
General protein information	Preferred Names Rap guanine nucleotide exchange factor 2 Names rap guanine nucleotide exchange factor 2 Cyclic nucleotide ras GEF Neural RAP guanine nucleotide exchange protein PDZ domain-containing guanine nucleotide exchange factor 1 Rap guanine nucleotide exchange factor (GEF) 2 Ras/Rap1-associating GEF-1 nRap GEP
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	RAPGEF2	NP_055062.1
	RAPGEF2	XP_001093090.2
Mus musculus (house mouse)	Rapgef2	NP_001093094.2
Rattus norvegicus (Norway rat)	Rapgef2	NP_001101154.1
Gallus gallus (chicken)	RAPGEF2	XP_004940966.1
Xenopus tropicalis (tropical clawed frog)	rapgef2	NP_001120522.1
Pan troglodytes (chimpanzee)	RAPGEF2	XP_001147382.2
Canis lupus familiaris (dog)	RAPGEF2	XP_005629442.1
Danio rerio (zebrafish)	rapgef2	XP_005173396.1
Bos taurus (cattle)	RAPGEF2	NP_001178255.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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