rapgef2 cDNA ORF clone, Danio rerio(zebrafish)

The following rapgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa55039 XM_017359182.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa55030 XM_017359172.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55031 XM_017359173.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55032 XM_017359174.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55033 XM_017359176.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55034 XM_017359177.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55035 XM_017359178.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55036 XM_017359179.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55037 XM_017359180.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55038 XM_017359181.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa42644 XM_009291280.3
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa42652 XM_009291288.3
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa42652 XM_009291288.2 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa55039 XM_017359182.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa55033 XM_017359176.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55030 XM_017359172.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55031 XM_017359173.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55034 XM_017359177.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55036 XM_017359179.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55032 XM_017359174.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55035 XM_017359178.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa42644 XM_009291280.2 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa55037 XM_017359180.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55038 XM_017359181.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa55039
    Clone ID Related Accession (Same CDS sequence) XM_017359182.2 , XM_017359182.1
    Accession Version XM_017359182.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGCCAT TAGTCAGTGG TGGCGGCCAT GGAGTCTCCA GCCAGGCTGA TAAAAGCCCC 
    CTGCCGGCAG ACTTCAGCAG GTTACACCTG GCAGACGGGA TTCACCCTCA GGTGACACAC
    GTGTCCTCCA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGCAG CAGCCTGTCG
    GACATTTACC AGGCCACCGA GAGTGAGCCG GGCGACATGG ACCTCAGCGG TCTGCCTGAG
    ACGGCCGTTG ACTCCGAGGA GGACGATGAT GAGGAAGACA TCGAGCGTGC CTCAGATCCC
    CTCATGAGCC GAGACATCGT CCGAGACTGC CTGGAGAAAG ACCCCATGGA CCGAACAGAT
    GATGACATAG AGCAACTGTT GGAGTTCATG CACCAGCTAC CAGCCTTTGC CAACATGACC
    ATGTCTGTCA GGAGGGAGCT GTGTGCTGTC ATGGTGTTCG CTGTGGTGGA ACGGGCCGGC
    ACCATCGTCC TTAATGATGG CGAAGAGCTG GACTCGTGGT CTGTGATTCT GAATGGATCG
    GTGGAAGTGA CGCATCCAGA CAGCCGCAAT GAAATCCTCT GCATGGGCAA CAGCTTCGGC
    GTTTCTCCCA CCATGGAGAA AGAGTACATG AAGGGGGTGA TGAAGACCAA GGTTGATGAC
    TGCCAGTTTG TGTGCATCGC TCAGCAGGAT TACTGCTGCA TCCTCAACCA GGTGGAGAAG
    AACATGCAGA AGGTGGAGGA GGAAGGAGAG ATCGTGATGG TGAAGGAGCA CAGAGAGCTG
    GACCGCACAG GCACCAGGAA AGGGCACATC GTCATCAAGG GCACATCAGA GCGTCTCACC
    ATGCACCTTG TAGAAGAGCA CTCAGTGGTC GACCCGACTT ACATCGAGGA CTTCCTGCTC
    ACCTACCGCA CCTTCCTGCC GAGCCCCATG GTGGTGGGCA AGAGACTGTT GGAGTGGTTT
    CATGACCCCA GCCTCAGGGA CAAGGTTACA CGGGTAGTCT TGCTGTGGGT GAACAATCAC
    TTTAATGACT TTGAGGGCGA CCCTGCAATG ACACATTTTC TGGAGGAGTT CGAGTACAAT
    CTGGAGAGAG AGAAAATGTG TGGCCACCTA AGACTGTTAA ACATAGCATG TGCTGCCAAA
    GCTAAGCTGC GTGTGGTGAC CCTGACCAAA CCCTCGAGGG AAGCCCCGCT GGCCTTCACC
    CTGCTGGGCG GCTCCGAGAA GGGCTTTCGC ATCTTCATCG ACAGCGTGGA ACCCGGCAGT
    AAAGCTGCAG AGGCCGGCCT TAAAAGAGGA GATCAGATAC TGGAGGTAAA TGGGCAAAAC
    TTTGAAAATG TGCCGCTATC CAAAGCCAAC GAGATTCTCA AAAACAACAC GCACCTGTCC
    ATTACTGTGA AAACCAATCT CTTGGTGTTT AAGGAGCTGC TGGCCAGGCC TGACCAGGAT
    CAGGAGCAAG AACAAGAAGA GGAGCCCGAC CGGAAAAACG GGGCTCCGCA CATCCCTAAA
    ATCGGGGACA TTAAGAAAGC CAGCCGCTAC TCCATCCCTG ACCTGGCTGT GGACGTGGAG
    CAGGTCATGG ATCTGGAGAA AGCCAGTAAG AAAGCCAAGG CTAACAGCGC AGGCGGAAGA
    AACAAGCTGA AGAAGATCTT CGACAAGACA CTGACCAGTA TTCTTCCACC CAAACCTTAC
    AATGACGTTG GGGTGGGACA GTCCCAGGAT GACAGTATTG TGGGCCTGAA GCAGTCAAAG
    CAGATTCCTC CTGCTCTCCC TGTCAGTGGA AACCTTTCAT CAAGCAACCC TGACCTGCTG
    CAGTCACACC ATCGCATACT CGACTTCAAC AACCAGCCTG AAGCGGTCCC TATAAACATG
    TCAGACCAGG TATTGCGAGT GTTCAAGGCG GACCAGCAAA GTCGGTACAT CATGATTGGG
    AAGGACACCA CGGCAAAAGA AGTGGTGGCT CAGGCCATCC GAGAGTTCGC CCTCACCGCT
    GCTGCCGAAG CCTATTCGTT GTGTGAGGTG TCAGTCACTC CCGAAGGAGT CATCAAGCAA
    AGGAGACTCC CTGAACAACT CTCCAAACTG GCTGACAGGA TCCAGCTGAG TGGAAGGTAC
    TACCTGAAAA GCAACATGGA GACAGAGACA CTGTGCTCGG ATGAAGATGC GCAGGAGTTG
    CTACGAGAGA GCCATATCAG CCTGCTGCAG CTCAGCACGG TGGAAGTGGC AACCCAACTC
    TCCATGCGCG CCTTTGAGCT GTTCTGTGCC ATCGAGCCCA CCGAGTACAT CGATGACCTG
    TTTAAGCTGA AAACTCGCCT CACCGGACCC CCCAGCCTCA AGCTTTTTGA GGAGGCCATC
    AATCGGGAGA CTTTCTGGGT GGCCACAGAG GTGGTGCGAG AGGCCAACCA GCTCAAACGC
    ATGAAGATTG TCAAGCACTT CATTAAGATT GCACTGCACT GCCGCGAATG CAAGAACTTC
    AACTCCATGT TTGCCATCAT CAGTGGTCTG AACTTGGCAC CAGTGTCCAG GCTGAGAGGC
    ACATGGGAGA AGCTGCCCAG CAAATACGAG AAGCTGTTTA GCGATCTTCA AGACCTCTTC
    GATCCCTCCA GGAATATGGC CAAGTATCGC AACCTTCTAA ACAGCCAGAA CCTGCAGCCC
    CCTATCATCC CACTCTTCCC CGTAATCAAG AAGGATCTCA CTTTCCTGCA TGAAGGGAAT
    GACTCCAAAG TGGACGGTTT GGTGAACTTT GAGAAGCTGC GGATGATCGC TAAAGAGATC
    CGACACGTGG GCAGAATGGC AGCCGTAAAC ATGGACCCTG CACTGATGTT CAGAACCAGG
    AAGAAGAAAT GGAGGAGTTT AGGGTCACTC AGCCAGGGCA GTGCCAACGC GGCTGTCCTG
    GATGTGACCC AGACGGGTGG GCACAAGAAG CGGGTCAGAA GGAGTTCCTT CCTTAATGCC
    AAGAAGCTGT ACGAGGACGC ACAGATGGCA CGCAAAGTCA AACAATACCT GTCCAACCTC
    ACCCTGGAGA CCAACGAGGA GAGCCTGCAA ACGCTCTCAA TGCAGTGTGA GCCCTCAATC
    AACACCTTGC CGAAGAGTTC TGGGGACAGA AAGAGACCCG ACACATCACC CGTAGTCTCA
    CGGGCTGCGA CCCACCAGCG CCAGCAGCTT CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT
    GCCGTAGCCC TCTACCCATC CCGCAAAAAA GTGCCAGTCA AAGACTTACC GCCATTTGGC
    ACAAGCTCAC CACAGTCTCT GAAGAAGATC CTGTCCCTTT CAGAGGAGGC AGGAGACAGA
    CACCGAAAGC AGGCAGAGGA CAGTGTGTCC AACAACTCCT CACAGCTCTC GTCTCCTCCA
    ACATCTCCTC ACAGCTCACC CAAAAAAGCC ATCGCATCAA TAAGAAGTCA TGGTATGAAG
    GGCTTTTCCT CCTTGTGTAG TAAATCACCA ACAGGTTATG GACGAGCTGG TGATTACCTG
    TCCGACTCTG GCCACAGTGA GATCTCCTCC CGCTCCAGTC TGGTGAGCAC TTCTTCTCTG
    GACATGGGGC AGGACGAGCG CCGGCAGCGT TACGGAGGAA CGGCCGGAGA AGGACACGGC
    AGTGGCCACC GGCTCGAGCG CAGAGCTACT GCTGACCCTG ACCAGTACAG CTTGGGGTCC
    TACTCCTCCA TGCAGGACTG TCGTGGTCTG TACGCAGGCG CCACCGTCCT GTCCTCACCC
    AGCTCAGAGG AGCTCACGCA GGACCAGGGT GACCGGGTGT CCCTGGACGC TGCGGATTCC
    GGTCGTGGCA GCTGGACTTC GTGCTCCAGC GGCTCTCATG ATAACATCCA GACCATGCAG
    CAGGGCCGCA GCTGGGAGGC GCTGGCAGAG GGCGCAGGCC TGTGGGGCAG CAGAGGGAGC
    TGGGCATCCG CATGCTCCTC TTCTTCTTCG GTGGCATGTT GGGGCGAGGA CTCTGAAGGC
    GACACTGGCA CCATCAAACG GAGAGGAGGG AGGGATGTGA CCACAGATCC AGAGACCAGC
    AGCATCACCT CCACCGGGTC GGAAGAGTCC AGACAGCACA GCCGACGGCC CTCACCCATC
    ACCGCAGGAA ACACCAAGGC TTCCATCACA CGGAAGGACG GCCGCTATCG GGACCCTCCA
    CCAACGCCGC CGGGTTACAC CGCGCTGACC ATCACAGATG TGACCGAGGG AACGCCACAT
    TCGGGCAGGA AACCACCAGA CTACACCACC GCCCTGCAGC GCTCGCGCCT CGTCACACAC
    TCCCTCGACT CCCAGCAGCC CCCAGCCGCA GGGAAACCGG ACCCCCGGGG CCGAGAGGAG
    GACGAGGCAG TGGAGGTGGA GGAGGAGGGT GAGTGCTTGT CTCCCAAACT CAGAGCTCAG
    AGGAGGAGAG GTCCACACAC AGCAGTTCCC CCCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214671.1
    CDS736..5157
    Translation

    Target ORF information:

    RefSeq Version XM_017359182.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359182.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGCCAT TAGTCAGTGG TGGCGGCCAT GGAGTCTCCA GCCAGGCTGA TAAAAGCCCC 
    CTGCCGGCAG ACTTCAGCAG GTTACACCTG GCAGACGGGA TTCACCCTCA GGTGACACAC
    GTGTCCTCCA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGCAG CAGCCTGTCG
    GACATTTACC AGGCCACCGA GAGTGAGCCG GGCGACATGG ACCTCAGCGG TCTGCCTGAG
    ACGGCCGTTG ACTCCGAGGA GGACGATGAT GAGGAAGACA TCGAGCGTGC CTCAGATCCC
    CTCATGAGCC GAGACATCGT CCGAGACTGC CTGGAGAAAG ACCCCATGGA CCGAACAGAT
    GATGACATAG AGCAACTGTT GGAGTTCATG CACCAGCTAC CAGCCTTTGC CAACATGACC
    ATGTCTGTCA GGAGGGAGCT GTGTGCTGTC ATGGTGTTCG CTGTGGTGGA ACGGGCCGGC
    ACCATCGTCC TTAATGATGG CGAAGAGCTG GACTCGTGGT CTGTGATTCT GAATGGATCG
    GTGGAAGTGA CGCATCCAGA CAGCCGCAAT GAAATCCTCT GCATGGGCAA CAGCTTCGGC
    GTTTCTCCCA CCATGGAGAA AGAGTACATG AAGGGGGTGA TGAAGACCAA GGTTGATGAC
    TGCCAGTTTG TGTGCATCGC TCAGCAGGAT TACTGCTGCA TCCTCAACCA GGTGGAGAAG
    AACATGCAGA AGGTGGAGGA GGAAGGAGAG ATCGTGATGG TGAAGGAGCA CAGAGAGCTG
    GACCGCACAG GCACCAGGAA AGGGCACATC GTCATCAAGG GCACATCAGA GCGTCTCACC
    ATGCACCTTG TAGAAGAGCA CTCAGTGGTC GACCCGACTT ACATCGAGGA CTTCCTGCTC
    ACCTACCGCA CCTTCCTGCC GAGCCCCATG GTGGTGGGCA AGAGACTGTT GGAGTGGTTT
    CATGACCCCA GCCTCAGGGA CAAGGTTACA CGGGTAGTCT TGCTGTGGGT GAACAATCAC
    TTTAATGACT TTGAGGGCGA CCCTGCAATG ACACATTTTC TGGAGGAGTT CGAGTACAAT
    CTGGAGAGAG AGAAAATGTG TGGCCACCTA AGACTGTTAA ACATAGCATG TGCTGCCAAA
    GCTAAGCTGC GTGTGGTGAC CCTGACCAAA CCCTCGAGGG AAGCCCCGCT GGCCTTCACC
    CTGCTGGGCG GCTCCGAGAA GGGCTTTCGC ATCTTCATCG ACAGCGTGGA ACCCGGCAGT
    AAAGCTGCAG AGGCCGGCCT TAAAAGAGGA GATCAGATAC TGGAGGTAAA TGGGCAAAAC
    TTTGAAAATG TGCCGCTATC CAAAGCCAAC GAGATTCTCA AAAACAACAC GCACCTGTCC
    ATTACTGTGA AAACCAATCT CTTGGTGTTT AAGGAGCTGC TGGCCAGGCC TGACCAGGAT
    CAGGAGCAAG AACAAGAAGA GGAGCCCGAC CGGAAAAACG GGGCTCCGCA CATCCCTAAA
    ATCGGGGACA TTAAGAAAGC CAGCCGCTAC TCCATCCCTG ACCTGGCTGT GGACGTGGAG
    CAGGTCATGG ATCTGGAGAA AGCCAGTAAG AAAGCCAAGG CTAACAGCGC AGGCGGAAGA
    AACAAGCTGA AGAAGATCTT CGACAAGACA CTGACCAGTA TTCTTCCACC CAAACCTTAC
    AATGACGTTG GGGTGGGACA GTCCCAGGAT GACAGTATTG TGGGCCTGAA GCAGTCAAAG
    CAGATTCCTC CTGCTCTCCC TGTCAGTGGA AACCTTTCAT CAAGCAACCC TGACCTGCTG
    CAGTCACACC ATCGCATACT CGACTTCAAC AACCAGCCTG AAGCGGTCCC TATAAACATG
    TCAGACCAGG TATTGCGAGT GTTCAAGGCG GACCAGCAAA GTCGGTACAT CATGATTGGG
    AAGGACACCA CGGCAAAAGA AGTGGTGGCT CAGGCCATCC GAGAGTTCGC CCTCACCGCT
    GCTGCCGAAG CCTATTCGTT GTGTGAGGTG TCAGTCACTC CCGAAGGAGT CATCAAGCAA
    AGGAGACTCC CTGAACAACT CTCCAAACTG GCTGACAGGA TCCAGCTGAG TGGAAGGTAC
    TACCTGAAAA GCAACATGGA GACAGAGACA CTGTGCTCGG ATGAAGATGC GCAGGAGTTG
    CTACGAGAGA GCCATATCAG CCTGCTGCAG CTCAGCACGG TGGAAGTGGC AACCCAACTC
    TCCATGCGCG CCTTTGAGCT GTTCTGTGCC ATCGAGCCCA CCGAGTACAT CGATGACCTG
    TTTAAGCTGA AAACTCGCCT CACCGGACCC CCCAGCCTCA AGCTTTTTGA GGAGGCCATC
    AATCGGGAGA CTTTCTGGGT GGCCACAGAG GTGGTGCGAG AGGCCAACCA GCTCAAACGC
    ATGAAGATTG TCAAGCACTT CATTAAGATT GCACTGCACT GCCGCGAATG CAAGAACTTC
    AACTCCATGT TTGCCATCAT CAGTGGTCTG AACTTGGCAC CAGTGTCCAG GCTGAGAGGC
    ACATGGGAGA AGCTGCCCAG CAAATACGAG AAGCTGTTTA GCGATCTTCA AGACCTCTTC
    GATCCCTCCA GGAATATGGC CAAGTATCGC AACCTTCTAA ACAGCCAGAA CCTGCAGCCC
    CCTATCATCC CACTCTTCCC CGTAATCAAG AAGGATCTCA CTTTCCTGCA TGAAGGGAAT
    GACTCCAAAG TGGACGGTTT GGTGAACTTT GAGAAGCTGC GGATGATCGC TAAAGAGATC
    CGACACGTGG GCAGAATGGC AGCCGTAAAC ATGGACCCTG CACTGATGTT CAGAACCAGG
    AAGAAGAAAT GGAGGAGTTT AGGGTCACTC AGCCAGGGCA GTGCCAACGC GGCTGTCCTG
    GATGTGACCC AGACGGGTGG GCACAAGAAG CGGGTCAGAA GGAGTTCCTT CCTTAATGCC
    AAGAAGCTGT ACGAGGACGC ACAGATGGCA CGCAAAGTCA AACAATACCT GTCCAACCTC
    ACCCTGGAGA CCAACGAGGA GAGCCTGCAA ACGCTCTCAA TGCAGTGTGA GCCCTCAATC
    AACACCTTGC CGAAGAGTTC TGGGGACAGA AAGAGACCCG ACACATCACC CGTAGTCTCA
    CGGGCTGCGA CCCACCAGCG CCAGCAGCTT CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT
    GCCGTAGCCC TCTACCCATC CCGCAAAAAA GTGCCAGTCA AAGACTTACC GCCATTTGGC
    ACAAGCTCAC CACAGTCTCT GAAGAAGATC CTGTCCCTTT CAGAGGAGGC AGGAGACAGA
    CACCGAAAGC AGGCAGAGGA CAGTGTGTCC AACAACTCCT CACAGCTCTC GTCTCCTCCA
    ACATCTCCTC ACAGCTCACC CAAAAAAGCC ATCGCATCAA TAAGAAGTCA TGGTATGAAG
    GGCTTTTCCT CCTTGTGTAG TAAATCACCA ACAGGTTATG GACGAGCTGG TGATTACCTG
    TCCGACTCTG GCCACAGTGA GATCTCCTCC CGCTCCAGTC TGGTGAGCAC TTCTTCTCTG
    GACATGGGGC AGGACGAGCG CCGGCAGCGT TACGGAGGAA CGGCCGGAGA AGGACACGGC
    AGTGGCCACC GGCTCGAGCG CAGAGCTACT GCTGACCCTG ACCAGTACAG CTTGGGGTCC
    TACTCCTCCA TGCAGGACTG TCGTGGTCTG TACGCAGGCG CCACCGTCCT GTCCTCACCC
    AGCTCAGAGG AGCTCACGCA GGACCAGGGT GACCGGGTGT CCCTGGACGC TGCGGATTCC
    GGTCGTGGCA GCTGGACTTC GTGCTCCAGC GGCTCTCATG ATAACATCCA GACCATGCAG
    CAGGGCCGCA GCTGGGAGGC GCTGGCAGAG GGCGCAGGCC TGTGGGGCAG CAGAGGGAGC
    TGGGCATCCG CATGCTCCTC TTCTTCTTCG GTGGCATGTT GGGGCGAGGA CTCTGAAGGC
    GACACTGGCA CCATCAAACG GAGAGGAGGG AGGGATGTGA CCACAGATCC AGAGACCAGC
    AGCATCACCT CCACCGGGTC GGAAGAGTCC AGACAGCACA GCCGACGGCC CTCACCCATC
    ACCGCAGGAA ACACCAAGGC TTCCATCACA CGGAAGGACG GCCGCTATCG GGACCCTCCA
    CCAACGCCGC CGGGTTACAC CGCGCTGACC ATCACAGATG TGACCGAGGG AACGCCACAT
    TCGGGCAGGA AACCACCAGA CTACACCACC GCCCTGCAGC GCTCGCGCCT CGTCACACAC
    TCCCTCGACT CCCAGCAGCC CCCAGCCGCA GGGAAACCGG ACCCCCGGGG CCGAGAGGAG
    GACGAGGCAG TGGAGGTGGA GGAGGAGGGT GAGTGCTTGT CTCCCAAACT CAGAGCTCAG
    AGGAGGAGAG GTCCACACAC AGCAGTTCCC CCCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55039
    Clone ID Related Accession (Same CDS sequence) XM_017359182.2 , XM_017359182.1
    Accession Version XM_017359182.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGCCAT TAGTCAGTGG TGGCGGCCAT GGAGTCTCCA GCCAGGCTGA TAAAAGCCCC 
    CTGCCGGCAG ACTTCAGCAG GTTACACCTG GCAGACGGGA TTCACCCTCA GGTGACACAC
    GTGTCCTCCA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGCAG CAGCCTGTCG
    GACATTTACC AGGCCACCGA GAGTGAGCCG GGCGACATGG ACCTCAGCGG TCTGCCTGAG
    ACGGCCGTTG ACTCCGAGGA GGACGATGAT GAGGAAGACA TCGAGCGTGC CTCAGATCCC
    CTCATGAGCC GAGACATCGT CCGAGACTGC CTGGAGAAAG ACCCCATGGA CCGAACAGAT
    GATGACATAG AGCAACTGTT GGAGTTCATG CACCAGCTAC CAGCCTTTGC CAACATGACC
    ATGTCTGTCA GGAGGGAGCT GTGTGCTGTC ATGGTGTTCG CTGTGGTGGA ACGGGCCGGC
    ACCATCGTCC TTAATGATGG CGAAGAGCTG GACTCGTGGT CTGTGATTCT GAATGGATCG
    GTGGAAGTGA CGCATCCAGA CAGCCGCAAT GAAATCCTCT GCATGGGCAA CAGCTTCGGC
    GTTTCTCCCA CCATGGAGAA AGAGTACATG AAGGGGGTGA TGAAGACCAA GGTTGATGAC
    TGCCAGTTTG TGTGCATCGC TCAGCAGGAT TACTGCTGCA TCCTCAACCA GGTGGAGAAG
    AACATGCAGA AGGTGGAGGA GGAAGGAGAG ATCGTGATGG TGAAGGAGCA CAGAGAGCTG
    GACCGCACAG GCACCAGGAA AGGGCACATC GTCATCAAGG GCACATCAGA GCGTCTCACC
    ATGCACCTTG TAGAAGAGCA CTCAGTGGTC GACCCGACTT ACATCGAGGA CTTCCTGCTC
    ACCTACCGCA CCTTCCTGCC GAGCCCCATG GTGGTGGGCA AGAGACTGTT GGAGTGGTTT
    CATGACCCCA GCCTCAGGGA CAAGGTTACA CGGGTAGTCT TGCTGTGGGT GAACAATCAC
    TTTAATGACT TTGAGGGCGA CCCTGCAATG ACACATTTTC TGGAGGAGTT CGAGTACAAT
    CTGGAGAGAG AGAAAATGTG TGGCCACCTA AGACTGTTAA ACATAGCATG TGCTGCCAAA
    GCTAAGCTGC GTGTGGTGAC CCTGACCAAA CCCTCGAGGG AAGCCCCGCT GGCCTTCACC
    CTGCTGGGCG GCTCCGAGAA GGGCTTTCGC ATCTTCATCG ACAGCGTGGA ACCCGGCAGT
    AAAGCTGCAG AGGCCGGCCT TAAAAGAGGA GATCAGATAC TGGAGGTAAA TGGGCAAAAC
    TTTGAAAATG TGCCGCTATC CAAAGCCAAC GAGATTCTCA AAAACAACAC GCACCTGTCC
    ATTACTGTGA AAACCAATCT CTTGGTGTTT AAGGAGCTGC TGGCCAGGCC TGACCAGGAT
    CAGGAGCAAG AACAAGAAGA GGAGCCCGAC CGGAAAAACG GGGCTCCGCA CATCCCTAAA
    ATCGGGGACA TTAAGAAAGC CAGCCGCTAC TCCATCCCTG ACCTGGCTGT GGACGTGGAG
    CAGGTCATGG ATCTGGAGAA AGCCAGTAAG AAAGCCAAGG CTAACAGCGC AGGCGGAAGA
    AACAAGCTGA AGAAGATCTT CGACAAGACA CTGACCAGTA TTCTTCCACC CAAACCTTAC
    AATGACGTTG GGGTGGGACA GTCCCAGGAT GACAGTATTG TGGGCCTGAA GCAGTCAAAG
    CAGATTCCTC CTGCTCTCCC TGTCAGTGGA AACCTTTCAT CAAGCAACCC TGACCTGCTG
    CAGTCACACC ATCGCATACT CGACTTCAAC AACCAGCCTG AAGCGGTCCC TATAAACATG
    TCAGACCAGG TATTGCGAGT GTTCAAGGCG GACCAGCAAA GTCGGTACAT CATGATTGGG
    AAGGACACCA CGGCAAAAGA AGTGGTGGCT CAGGCCATCC GAGAGTTCGC CCTCACCGCT
    GCTGCCGAAG CCTATTCGTT GTGTGAGGTG TCAGTCACTC CCGAAGGAGT CATCAAGCAA
    AGGAGACTCC CTGAACAACT CTCCAAACTG GCTGACAGGA TCCAGCTGAG TGGAAGGTAC
    TACCTGAAAA GCAACATGGA GACAGAGACA CTGTGCTCGG ATGAAGATGC GCAGGAGTTG
    CTACGAGAGA GCCATATCAG CCTGCTGCAG CTCAGCACGG TGGAAGTGGC AACCCAACTC
    TCCATGCGCG CCTTTGAGCT GTTCTGTGCC ATCGAGCCCA CCGAGTACAT CGATGACCTG
    TTTAAGCTGA AAACTCGCCT CACCGGACCC CCCAGCCTCA AGCTTTTTGA GGAGGCCATC
    AATCGGGAGA CTTTCTGGGT GGCCACAGAG GTGGTGCGAG AGGCCAACCA GCTCAAACGC
    ATGAAGATTG TCAAGCACTT CATTAAGATT GCACTGCACT GCCGCGAATG CAAGAACTTC
    AACTCCATGT TTGCCATCAT CAGTGGTCTG AACTTGGCAC CAGTGTCCAG GCTGAGAGGC
    ACATGGGAGA AGCTGCCCAG CAAATACGAG AAGCTGTTTA GCGATCTTCA AGACCTCTTC
    GATCCCTCCA GGAATATGGC CAAGTATCGC AACCTTCTAA ACAGCCAGAA CCTGCAGCCC
    CCTATCATCC CACTCTTCCC CGTAATCAAG AAGGATCTCA CTTTCCTGCA TGAAGGGAAT
    GACTCCAAAG TGGACGGTTT GGTGAACTTT GAGAAGCTGC GGATGATCGC TAAAGAGATC
    CGACACGTGG GCAGAATGGC AGCCGTAAAC ATGGACCCTG CACTGATGTT CAGAACCAGG
    AAGAAGAAAT GGAGGAGTTT AGGGTCACTC AGCCAGGGCA GTGCCAACGC GGCTGTCCTG
    GATGTGACCC AGACGGGTGG GCACAAGAAG CGGGTCAGAA GGAGTTCCTT CCTTAATGCC
    AAGAAGCTGT ACGAGGACGC ACAGATGGCA CGCAAAGTCA AACAATACCT GTCCAACCTC
    ACCCTGGAGA CCAACGAGGA GAGCCTGCAA ACGCTCTCAA TGCAGTGTGA GCCCTCAATC
    AACACCTTGC CGAAGAGTTC TGGGGACAGA AAGAGACCCG ACACATCACC CGTAGTCTCA
    CGGGCTGCGA CCCACCAGCG CCAGCAGCTT CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT
    GCCGTAGCCC TCTACCCATC CCGCAAAAAA GTGCCAGTCA AAGACTTACC GCCATTTGGC
    ACAAGCTCAC CACAGTCTCT GAAGAAGATC CTGTCCCTTT CAGAGGAGGC AGGAGACAGA
    CACCGAAAGC AGGCAGAGGA CAGTGTGTCC AACAACTCCT CACAGCTCTC GTCTCCTCCA
    ACATCTCCTC ACAGCTCACC CAAAAAAGCC ATCGCATCAA TAAGAAGTCA TGGTATGAAG
    GGCTTTTCCT CCTTGTGTAG TAAATCACCA ACAGGTTATG GACGAGCTGG TGATTACCTG
    TCCGACTCTG GCCACAGTGA GATCTCCTCC CGCTCCAGTC TGGTGAGCAC TTCTTCTCTG
    GACATGGGGC AGGACGAGCG CCGGCAGCGT TACGGAGGAA CGGCCGGAGA AGGACACGGC
    AGTGGCCACC GGCTCGAGCG CAGAGCTACT GCTGACCCTG ACCAGTACAG CTTGGGGTCC
    TACTCCTCCA TGCAGGACTG TCGTGGTCTG TACGCAGGCG CCACCGTCCT GTCCTCACCC
    AGCTCAGAGG AGCTCACGCA GGACCAGGGT GACCGGGTGT CCCTGGACGC TGCGGATTCC
    GGTCGTGGCA GCTGGACTTC GTGCTCCAGC GGCTCTCATG ATAACATCCA GACCATGCAG
    CAGGGCCGCA GCTGGGAGGC GCTGGCAGAG GGCGCAGGCC TGTGGGGCAG CAGAGGGAGC
    TGGGCATCCG CATGCTCCTC TTCTTCTTCG GTGGCATGTT GGGGCGAGGA CTCTGAAGGC
    GACACTGGCA CCATCAAACG GAGAGGAGGG AGGGATGTGA CCACAGATCC AGAGACCAGC
    AGCATCACCT CCACCGGGTC GGAAGAGTCC AGACAGCACA GCCGACGGCC CTCACCCATC
    ACCGCAGGAA ACACCAAGGC TTCCATCACA CGGAAGGACG GCCGCTATCG GGACCCTCCA
    CCAACGCCGC CGGGTTACAC CGCGCTGACC ATCACAGATG TGACCGAGGG AACGCCACAT
    TCGGGCAGGA AACCACCAGA CTACACCACC GCCCTGCAGC GCTCGCGCCT CGTCACACAC
    TCCCTCGACT CCCAGCAGCC CCCAGCCGCA GGGAAACCGG ACCCCCGGGG CCGAGAGGAG
    GACGAGGCAG TGGAGGTGGA GGAGGAGGGT GAGTGCTTGT CTCCCAAACT CAGAGCTCAG
    AGGAGGAGAG GTCCACACAC AGCAGTTCCC CCCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214671.1
    CDS754..5175
    Translation

    Target ORF information:

    RefSeq Version XM_017359182.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGCCAT TAGTCAGTGG TGGCGGCCAT GGAGTCTCCA GCCAGGCTGA TAAAAGCCCC 
    CTGCCGGCAG ACTTCAGCAG GTTACACCTG GCAGACGGGA TTCACCCTCA GGTGACACAC
    GTGTCCTCCA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGCAG CAGCCTGTCG
    GACATTTACC AGGCCACCGA GAGTGAGCCG GGCGACATGG ACCTCAGCGG TCTGCCTGAG
    ACGGCCGTTG ACTCCGAGGA GGACGATGAT GAGGAAGACA TCGAGCGTGC CTCAGATCCC
    CTCATGAGCC GAGACATCGT CCGAGACTGC CTGGAGAAAG ACCCCATGGA CCGAACAGAT
    GATGACATAG AGCAACTGTT GGAGTTCATG CACCAGCTAC CAGCCTTTGC CAACATGACC
    ATGTCTGTCA GGAGGGAGCT GTGTGCTGTC ATGGTGTTCG CTGTGGTGGA ACGGGCCGGC
    ACCATCGTCC TTAATGATGG CGAAGAGCTG GACTCGTGGT CTGTGATTCT GAATGGATCG
    GTGGAAGTGA CGCATCCAGA CAGCCGCAAT GAAATCCTCT GCATGGGCAA CAGCTTCGGC
    GTTTCTCCCA CCATGGAGAA AGAGTACATG AAGGGGGTGA TGAAGACCAA GGTTGATGAC
    TGCCAGTTTG TGTGCATCGC TCAGCAGGAT TACTGCTGCA TCCTCAACCA GGTGGAGAAG
    AACATGCAGA AGGTGGAGGA GGAAGGAGAG ATCGTGATGG TGAAGGAGCA CAGAGAGCTG
    GACCGCACAG GCACCAGGAA AGGGCACATC GTCATCAAGG GCACATCAGA GCGTCTCACC
    ATGCACCTTG TAGAAGAGCA CTCAGTGGTC GACCCGACTT ACATCGAGGA CTTCCTGCTC
    ACCTACCGCA CCTTCCTGCC GAGCCCCATG GTGGTGGGCA AGAGACTGTT GGAGTGGTTT
    CATGACCCCA GCCTCAGGGA CAAGGTTACA CGGGTAGTCT TGCTGTGGGT GAACAATCAC
    TTTAATGACT TTGAGGGCGA CCCTGCAATG ACACATTTTC TGGAGGAGTT CGAGTACAAT
    CTGGAGAGAG AGAAAATGTG TGGCCACCTA AGACTGTTAA ACATAGCATG TGCTGCCAAA
    GCTAAGCTGC GTGTGGTGAC CCTGACCAAA CCCTCGAGGG AAGCCCCGCT GGCCTTCACC
    CTGCTGGGCG GCTCCGAGAA GGGCTTTCGC ATCTTCATCG ACAGCGTGGA ACCCGGCAGT
    AAAGCTGCAG AGGCCGGCCT TAAAAGAGGA GATCAGATAC TGGAGGTAAA TGGGCAAAAC
    TTTGAAAATG TGCCGCTATC CAAAGCCAAC GAGATTCTCA AAAACAACAC GCACCTGTCC
    ATTACTGTGA AAACCAATCT CTTGGTGTTT AAGGAGCTGC TGGCCAGGCC TGACCAGGAT
    CAGGAGCAAG AACAAGAAGA GGAGCCCGAC CGGAAAAACG GGGCTCCGCA CATCCCTAAA
    ATCGGGGACA TTAAGAAAGC CAGCCGCTAC TCCATCCCTG ACCTGGCTGT GGACGTGGAG
    CAGGTCATGG ATCTGGAGAA AGCCAGTAAG AAAGCCAAGG CTAACAGCGC AGGCGGAAGA
    AACAAGCTGA AGAAGATCTT CGACAAGACA CTGACCAGTA TTCTTCCACC CAAACCTTAC
    AATGACGTTG GGGTGGGACA GTCCCAGGAT GACAGTATTG TGGGCCTGAA GCAGTCAAAG
    CAGATTCCTC CTGCTCTCCC TGTCAGTGGA AACCTTTCAT CAAGCAACCC TGACCTGCTG
    CAGTCACACC ATCGCATACT CGACTTCAAC AACCAGCCTG AAGCGGTCCC TATAAACATG
    TCAGACCAGG TATTGCGAGT GTTCAAGGCG GACCAGCAAA GTCGGTACAT CATGATTGGG
    AAGGACACCA CGGCAAAAGA AGTGGTGGCT CAGGCCATCC GAGAGTTCGC CCTCACCGCT
    GCTGCCGAAG CCTATTCGTT GTGTGAGGTG TCAGTCACTC CCGAAGGAGT CATCAAGCAA
    AGGAGACTCC CTGAACAACT CTCCAAACTG GCTGACAGGA TCCAGCTGAG TGGAAGGTAC
    TACCTGAAAA GCAACATGGA GACAGAGACA CTGTGCTCGG ATGAAGATGC GCAGGAGTTG
    CTACGAGAGA GCCATATCAG CCTGCTGCAG CTCAGCACGG TGGAAGTGGC AACCCAACTC
    TCCATGCGCG CCTTTGAGCT GTTCTGTGCC ATCGAGCCCA CCGAGTACAT CGATGACCTG
    TTTAAGCTGA AAACTCGCCT CACCGGACCC CCCAGCCTCA AGCTTTTTGA GGAGGCCATC
    AATCGGGAGA CTTTCTGGGT GGCCACAGAG GTGGTGCGAG AGGCCAACCA GCTCAAACGC
    ATGAAGATTG TCAAGCACTT CATTAAGATT GCACTGCACT GCCGCGAATG CAAGAACTTC
    AACTCCATGT TTGCCATCAT CAGTGGTCTG AACTTGGCAC CAGTGTCCAG GCTGAGAGGC
    ACATGGGAGA AGCTGCCCAG CAAATACGAG AAGCTGTTTA GCGATCTTCA AGACCTCTTC
    GATCCCTCCA GGAATATGGC CAAGTATCGC AACCTTCTAA ACAGCCAGAA CCTGCAGCCC
    CCTATCATCC CACTCTTCCC CGTAATCAAG AAGGATCTCA CTTTCCTGCA TGAAGGGAAT
    GACTCCAAAG TGGACGGTTT GGTGAACTTT GAGAAGCTGC GGATGATCGC TAAAGAGATC
    CGACACGTGG GCAGAATGGC AGCCGTAAAC ATGGACCCTG CACTGATGTT CAGAACCAGG
    AAGAAGAAAT GGAGGAGTTT AGGGTCACTC AGCCAGGGCA GTGCCAACGC GGCTGTCCTG
    GATGTGACCC AGACGGGTGG GCACAAGAAG CGGGTCAGAA GGAGTTCCTT CCTTAATGCC
    AAGAAGCTGT ACGAGGACGC ACAGATGGCA CGCAAAGTCA AACAATACCT GTCCAACCTC
    ACCCTGGAGA CCAACGAGGA GAGCCTGCAA ACGCTCTCAA TGCAGTGTGA GCCCTCAATC
    AACACCTTGC CGAAGAGTTC TGGGGACAGA AAGAGACCCG ACACATCACC CGTAGTCTCA
    CGGGCTGCGA CCCACCAGCG CCAGCAGCTT CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT
    GCCGTAGCCC TCTACCCATC CCGCAAAAAA GTGCCAGTCA AAGACTTACC GCCATTTGGC
    ACAAGCTCAC CACAGTCTCT GAAGAAGATC CTGTCCCTTT CAGAGGAGGC AGGAGACAGA
    CACCGAAAGC AGGCAGAGGA CAGTGTGTCC AACAACTCCT CACAGCTCTC GTCTCCTCCA
    ACATCTCCTC ACAGCTCACC CAAAAAAGCC ATCGCATCAA TAAGAAGTCA TGGTATGAAG
    GGCTTTTCCT CCTTGTGTAG TAAATCACCA ACAGGTTATG GACGAGCTGG TGATTACCTG
    TCCGACTCTG GCCACAGTGA GATCTCCTCC CGCTCCAGTC TGGTGAGCAC TTCTTCTCTG
    GACATGGGGC AGGACGAGCG CCGGCAGCGT TACGGAGGAA CGGCCGGAGA AGGACACGGC
    AGTGGCCACC GGCTCGAGCG CAGAGCTACT GCTGACCCTG ACCAGTACAG CTTGGGGTCC
    TACTCCTCCA TGCAGGACTG TCGTGGTCTG TACGCAGGCG CCACCGTCCT GTCCTCACCC
    AGCTCAGAGG AGCTCACGCA GGACCAGGGT GACCGGGTGT CCCTGGACGC TGCGGATTCC
    GGTCGTGGCA GCTGGACTTC GTGCTCCAGC GGCTCTCATG ATAACATCCA GACCATGCAG
    CAGGGCCGCA GCTGGGAGGC GCTGGCAGAG GGCGCAGGCC TGTGGGGCAG CAGAGGGAGC
    TGGGCATCCG CATGCTCCTC TTCTTCTTCG GTGGCATGTT GGGGCGAGGA CTCTGAAGGC
    GACACTGGCA CCATCAAACG GAGAGGAGGG AGGGATGTGA CCACAGATCC AGAGACCAGC
    AGCATCACCT CCACCGGGTC GGAAGAGTCC AGACAGCACA GCCGACGGCC CTCACCCATC
    ACCGCAGGAA ACACCAAGGC TTCCATCACA CGGAAGGACG GCCGCTATCG GGACCCTCCA
    CCAACGCCGC CGGGTTACAC CGCGCTGACC ATCACAGATG TGACCGAGGG AACGCCACAT
    TCGGGCAGGA AACCACCAGA CTACACCACC GCCCTGCAGC GCTCGCGCCT CGTCACACAC
    TCCCTCGACT CCCAGCAGCC CCCAGCCGCA GGGAAACCGG ACCCCCGGGG CCGAGAGGAG
    GACGAGGCAG TGGAGGTGGA GGAGGAGGGT GAGTGCTTGT CTCCCAAACT CAGAGCTCAG
    AGGAGGAGAG GTCCACACAC AGCAGTTCCC CCCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55030
    Clone ID Related Accession (Same CDS sequence) XM_017359172.1 , XM_017359172.2
    Accession Version XM_017359172.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT
    CAGAGGAGGA GAGGTCCACA CACAGCAGTT CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214661.1
    CDS607..5511
    Translation

    Target ORF information:

    RefSeq Version XM_017359172.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT
    CAGAGGAGGA GAGGTCCACA CACAGCAGTT CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55030
    Clone ID Related Accession (Same CDS sequence) XM_017359172.1 , XM_017359172.2
    Accession Version XM_017359172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT
    CAGAGGAGGA GAGGTCCACA CACAGCAGTT CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214661.1
    CDS543..5447
    Translation

    Target ORF information:

    RefSeq Version XM_017359172.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT
    CAGAGGAGGA GAGGTCCACA CACAGCAGTT CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55031
    Clone ID Related Accession (Same CDS sequence) XM_017359173.1 , XM_017359173.2
    Accession Version XM_017359173.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGCCAT CGCATCAATA AGAAGTCATG GTATGAAGGG CTTTTCCTCC
    TTGTGTAGTA AATCACCAAC AGGTTATGGA CGAGCTGGTG ATTACCTGTC CGACTCTGGC
    CACAGTGAGA TCTCCTCCCG CTCCAGTCTG GTGAGCACTT CTTCTCTGGA CATGGGGCAG
    GACGAGCGCC GGCAGCGTTA CGGAGGAACG GCCGGAGAAG GACACGGCAG TGGCCACCGG
    CTCGAGCGCA GAGCTACTGC TGACCCTGAC CAGTACAGCT TGGGGTCCTA CTCCTCCATG
    CAGGACTGTC GTGGTCTGTA CGCAGGCGCC ACCGTCCTGT CCTCACCCAG CTCAGAGGAG
    CTCACGCAGG ACCAGGGTGA CCGGGTGTCC CTGGACGCTG CGGATTCCGG TCGTGGCAGC
    TGGACTTCGT GCTCCAGCGG CTCTCATGAT AACATCCAGA CCATGCAGCA GGGCCGCAGC
    TGGGAGGCGC TGGCAGAGGG CGCAGGCCTG TGGGGCAGCA GAGGGAGCTG GGCATCCGCA
    TGCTCCTCTT CTTCTTCGGT GGCATGTTGG GGCGAGGACT CTGAAGGCGA CACTGGCACC
    ATCAAACGGA GAGGAGGGAG GGATGTGACC ACAGATCCAG AGACCAGCAG CATCACCTCC
    ACCGGGTCGG AAGAGTCCAG ACAGCACAGC CGACGGCCCT CACCCATCAC CGCAGGAAAC
    ACCAAGGCTT CCATCACACG GAAGGACGGC CGCTATCGGG ACCCTCCACC AACGCCGCCG
    GGTTACACCG CGCTGACCAT CACAGATGTG ACCGAGGGAA CGCCACATTC GGGCAGGAAA
    CCACCAGACT ACACCACCGC CCTGCAGCGC TCGCGCCTCG TCACACACTC CCTCGACTCC
    CAGCAGCCCC CAGCCGCAGG GAAACCGGAC CCCCGGGGCC GAGAGGAGGA CGAGGCAGTG
    GAGGTGGAGG AGGAGGGTGA GTGCTTGTCT CCCAAACTCA GAGCTCAGAG GAGGAGAGGT
    CCACACACAG CAGTTCCCCC CAGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214662.1
    CDS606..5495
    Translation

    Target ORF information:

    RefSeq Version XM_017359173.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGCCAT CGCATCAATA AGAAGTCATG GTATGAAGGG CTTTTCCTCC
    TTGTGTAGTA AATCACCAAC AGGTTATGGA CGAGCTGGTG ATTACCTGTC CGACTCTGGC
    CACAGTGAGA TCTCCTCCCG CTCCAGTCTG GTGAGCACTT CTTCTCTGGA CATGGGGCAG
    GACGAGCGCC GGCAGCGTTA CGGAGGAACG GCCGGAGAAG GACACGGCAG TGGCCACCGG
    CTCGAGCGCA GAGCTACTGC TGACCCTGAC CAGTACAGCT TGGGGTCCTA CTCCTCCATG
    CAGGACTGTC GTGGTCTGTA CGCAGGCGCC ACCGTCCTGT CCTCACCCAG CTCAGAGGAG
    CTCACGCAGG ACCAGGGTGA CCGGGTGTCC CTGGACGCTG CGGATTCCGG TCGTGGCAGC
    TGGACTTCGT GCTCCAGCGG CTCTCATGAT AACATCCAGA CCATGCAGCA GGGCCGCAGC
    TGGGAGGCGC TGGCAGAGGG CGCAGGCCTG TGGGGCAGCA GAGGGAGCTG GGCATCCGCA
    TGCTCCTCTT CTTCTTCGGT GGCATGTTGG GGCGAGGACT CTGAAGGCGA CACTGGCACC
    ATCAAACGGA GAGGAGGGAG GGATGTGACC ACAGATCCAG AGACCAGCAG CATCACCTCC
    ACCGGGTCGG AAGAGTCCAG ACAGCACAGC CGACGGCCCT CACCCATCAC CGCAGGAAAC
    ACCAAGGCTT CCATCACACG GAAGGACGGC CGCTATCGGG ACCCTCCACC AACGCCGCCG
    GGTTACACCG CGCTGACCAT CACAGATGTG ACCGAGGGAA CGCCACATTC GGGCAGGAAA
    CCACCAGACT ACACCACCGC CCTGCAGCGC TCGCGCCTCG TCACACACTC CCTCGACTCC
    CAGCAGCCCC CAGCCGCAGG GAAACCGGAC CCCCGGGGCC GAGAGGAGGA CGAGGCAGTG
    GAGGTGGAGG AGGAGGGTGA GTGCTTGTCT CCCAAACTCA GAGCTCAGAG GAGGAGAGGT
    CCACACACAG CAGTTCCCCC CAGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55031
    Clone ID Related Accession (Same CDS sequence) XM_017359173.1 , XM_017359173.2
    Accession Version XM_017359173.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGCCAT CGCATCAATA AGAAGTCATG GTATGAAGGG CTTTTCCTCC
    TTGTGTAGTA AATCACCAAC AGGTTATGGA CGAGCTGGTG ATTACCTGTC CGACTCTGGC
    CACAGTGAGA TCTCCTCCCG CTCCAGTCTG GTGAGCACTT CTTCTCTGGA CATGGGGCAG
    GACGAGCGCC GGCAGCGTTA CGGAGGAACG GCCGGAGAAG GACACGGCAG TGGCCACCGG
    CTCGAGCGCA GAGCTACTGC TGACCCTGAC CAGTACAGCT TGGGGTCCTA CTCCTCCATG
    CAGGACTGTC GTGGTCTGTA CGCAGGCGCC ACCGTCCTGT CCTCACCCAG CTCAGAGGAG
    CTCACGCAGG ACCAGGGTGA CCGGGTGTCC CTGGACGCTG CGGATTCCGG TCGTGGCAGC
    TGGACTTCGT GCTCCAGCGG CTCTCATGAT AACATCCAGA CCATGCAGCA GGGCCGCAGC
    TGGGAGGCGC TGGCAGAGGG CGCAGGCCTG TGGGGCAGCA GAGGGAGCTG GGCATCCGCA
    TGCTCCTCTT CTTCTTCGGT GGCATGTTGG GGCGAGGACT CTGAAGGCGA CACTGGCACC
    ATCAAACGGA GAGGAGGGAG GGATGTGACC ACAGATCCAG AGACCAGCAG CATCACCTCC
    ACCGGGTCGG AAGAGTCCAG ACAGCACAGC CGACGGCCCT CACCCATCAC CGCAGGAAAC
    ACCAAGGCTT CCATCACACG GAAGGACGGC CGCTATCGGG ACCCTCCACC AACGCCGCCG
    GGTTACACCG CGCTGACCAT CACAGATGTG ACCGAGGGAA CGCCACATTC GGGCAGGAAA
    CCACCAGACT ACACCACCGC CCTGCAGCGC TCGCGCCTCG TCACACACTC CCTCGACTCC
    CAGCAGCCCC CAGCCGCAGG GAAACCGGAC CCCCGGGGCC GAGAGGAGGA CGAGGCAGTG
    GAGGTGGAGG AGGAGGGTGA GTGCTTGTCT CCCAAACTCA GAGCTCAGAG GAGGAGAGGT
    CCACACACAG CAGTTCCCCC CAGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214662.1
    CDS542..5431
    Translation

    Target ORF information:

    RefSeq Version XM_017359173.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGCCAT CGCATCAATA AGAAGTCATG GTATGAAGGG CTTTTCCTCC
    TTGTGTAGTA AATCACCAAC AGGTTATGGA CGAGCTGGTG ATTACCTGTC CGACTCTGGC
    CACAGTGAGA TCTCCTCCCG CTCCAGTCTG GTGAGCACTT CTTCTCTGGA CATGGGGCAG
    GACGAGCGCC GGCAGCGTTA CGGAGGAACG GCCGGAGAAG GACACGGCAG TGGCCACCGG
    CTCGAGCGCA GAGCTACTGC TGACCCTGAC CAGTACAGCT TGGGGTCCTA CTCCTCCATG
    CAGGACTGTC GTGGTCTGTA CGCAGGCGCC ACCGTCCTGT CCTCACCCAG CTCAGAGGAG
    CTCACGCAGG ACCAGGGTGA CCGGGTGTCC CTGGACGCTG CGGATTCCGG TCGTGGCAGC
    TGGACTTCGT GCTCCAGCGG CTCTCATGAT AACATCCAGA CCATGCAGCA GGGCCGCAGC
    TGGGAGGCGC TGGCAGAGGG CGCAGGCCTG TGGGGCAGCA GAGGGAGCTG GGCATCCGCA
    TGCTCCTCTT CTTCTTCGGT GGCATGTTGG GGCGAGGACT CTGAAGGCGA CACTGGCACC
    ATCAAACGGA GAGGAGGGAG GGATGTGACC ACAGATCCAG AGACCAGCAG CATCACCTCC
    ACCGGGTCGG AAGAGTCCAG ACAGCACAGC CGACGGCCCT CACCCATCAC CGCAGGAAAC
    ACCAAGGCTT CCATCACACG GAAGGACGGC CGCTATCGGG ACCCTCCACC AACGCCGCCG
    GGTTACACCG CGCTGACCAT CACAGATGTG ACCGAGGGAA CGCCACATTC GGGCAGGAAA
    CCACCAGACT ACACCACCGC CCTGCAGCGC TCGCGCCTCG TCACACACTC CCTCGACTCC
    CAGCAGCCCC CAGCCGCAGG GAAACCGGAC CCCCGGGGCC GAGAGGAGGA CGAGGCAGTG
    GAGGTGGAGG AGGAGGGTGA GTGCTTGTCT CCCAAACTCA GAGCTCAGAG GAGGAGAGGT
    CCACACACAG CAGTTCCCCC CAGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55032
    Clone ID Related Accession (Same CDS sequence) XM_017359174.2 , XM_017359174.1
    Accession Version XM_017359174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGAAGCG GTCCCTATAA ACATGTCAGA CCAGGTATTG
    CGAGTGTTCA AGGCGGACCA GCAAAGTCGG TACATCATGA TTGGGAAGGA CACCACGGCA
    AAAGAAGTGG TGGCTCAGGC CATCCGAGAG TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT
    TCGTTGTGTG AGGTGTCAGT CACTCCCGAA GGAGTCATCA AGCAAAGGAG ACTCCCTGAA
    CAACTCTCCA AACTGGCTGA CAGGATCCAG CTGAGTGGAA GGTACTACCT GAAAAGCAAC
    ATGGAGACAG AGACACTGTG CTCGGATGAA GATGCGCAGG AGTTGCTACG AGAGAGCCAT
    ATCAGCCTGC TGCAGCTCAG CACGGTGGAA GTGGCAACCC AACTCTCCAT GCGCGCCTTT
    GAGCTGTTCT GTGCCATCGA GCCCACCGAG TACATCGATG ACCTGTTTAA GCTGAAAACT
    CGCCTCACCG GACCCCCCAG CCTCAAGCTT TTTGAGGAGG CCATCAATCG GGAGACTTTC
    TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC AACCAGCTCA AACGCATGAA GATTGTCAAG
    CACTTCATTA AGATTGCACT GCACTGCCGC GAATGCAAGA ACTTCAACTC CATGTTTGCC
    ATCATCAGTG GTCTGAACTT GGCACCAGTG TCCAGGCTGA GAGGCACATG GGAGAAGCTG
    CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT CTTCAAGACC TCTTCGATCC CTCCAGGAAT
    ATGGCCAAGT ATCGCAACCT TCTAAACAGC CAGAACCTGC AGCCCCCTAT CATCCCACTC
    TTCCCCGTAA TCAAGAAGGA TCTCACTTTC CTGCATGAAG GGAATGACTC CAAAGTGGAC
    GGTTTGGTGA ACTTTGAGAA GCTGCGGATG ATCGCTAAAG AGATCCGACA CGTGGGCAGA
    ATGGCAGCCG TAAACATGGA CCCTGCACTG ATGTTCAGAA CCAGGAAGAA GAAATGGAGG
    AGTTTAGGGT CACTCAGCCA GGGCAGTGCC AACGCGGCTG TCCTGGATGT GACCCAGACG
    GGTGGGCACA AGAAGCGGGT CAGAAGGAGT TCCTTCCTTA ATGCCAAGAA GCTGTACGAG
    GACGCACAGA TGGCACGCAA AGTCAAACAA TACCTGTCCA ACCTCACCCT GGAGACCAAC
    GAGGAGAGCC TGCAAACGCT CTCAATGCAG TGTGAGCCCT CAATCAACAC CTTGCCGAAG
    AGTTCTGGGG ACAGAAAGAG ACCCGACACA TCACCCGTAG TCTCACGGGC TGCGACCCAC
    CAGCGCCAGC AGCTTCAAAA AGCCAACCAG GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC
    CCATCCCGCA AAAAAGTGCC AGTCAAAGAC TTACCGCCAT TTGGCACAAG CTCACCACAG
    TCTCTGAAGA AGATCCTGTC CCTTTCAGAG GAGGCAGGAG ACAGACACCG AAAGCAGGCA
    GAGGACAGTG TGTCCAACAA CTCCTCACAG CTCTCGTCTC CTCCAACATC TCCTCACAGC
    TCACCCAAAA AAGCCATCGC ATCAATAAGA AGTCATGGTA TGAAGGGCTT TTCCTCCTTG
    TGTAGTAAAT CACCAACAGG TTATGGACGA GCTGGTGATT ACCTGTCCGA CTCTGGCCAC
    AGTGAGATCT CCTCCCGCTC CAGTCTGGTG AGCACTTCTT CTCTGGACAT GGGGCAGGAC
    GAGCGCCGGC AGCGTTACGG AGGAACGGCC GGAGAAGGAC ACGGCAGTGG CCACCGGCTC
    GAGCGCAGAG CTACTGCTGA CCCTGACCAG TACAGCTTGG GGTCCTACTC CTCCATGCAG
    GACTGTCGTG GTCTGTACGC AGGCGCCACC GTCCTGTCCT CACCCAGCTC AGAGGAGCTC
    ACGCAGGACC AGGGTGACCG GGTGTCCCTG GACGCTGCGG ATTCCGGTCG TGGCAGCTGG
    ACTTCGTGCT CCAGCGGCTC TCATGATAAC ATCCAGACCA TGCAGCAGGG CCGCAGCTGG
    GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG GGCAGCAGAG GGAGCTGGGC ATCCGCATGC
    TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC GAGGACTCTG AAGGCGACAC TGGCACCATC
    AAACGGAGAG GAGGGAGGGA TGTGACCACA GATCCAGAGA CCAGCAGCAT CACCTCCACC
    GGGTCGGAAG AGTCCAGACA GCACAGCCGA CGGCCCTCAC CCATCACCGC AGGAAACACC
    AAGGCTTCCA TCACACGGAA GGACGGCCGC TATCGGGACC CTCCACCAAC GCCGCCGGGT
    TACACCGCGC TGACCATCAC AGATGTGACC GAGGGAACGC CACATTCGGG CAGGAAACCA
    CCAGACTACA CCACCGCCCT GCAGCGCTCG CGCCTCGTCA CACACTCCCT CGACTCCCAG
    CAGCCCCCAG CCGCAGGGAA ACCGGACCCC CGGGGCCGAG AGGAGGACGA GGCAGTGGAG
    GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC AAACTCAGAG CTCAGAGGAG GAGAGGTCCA
    CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214663.1
    CDS543..5429
    Translation

    Target ORF information:

    RefSeq Version XM_017359174.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGAAGCG GTCCCTATAA ACATGTCAGA CCAGGTATTG
    CGAGTGTTCA AGGCGGACCA GCAAAGTCGG TACATCATGA TTGGGAAGGA CACCACGGCA
    AAAGAAGTGG TGGCTCAGGC CATCCGAGAG TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT
    TCGTTGTGTG AGGTGTCAGT CACTCCCGAA GGAGTCATCA AGCAAAGGAG ACTCCCTGAA
    CAACTCTCCA AACTGGCTGA CAGGATCCAG CTGAGTGGAA GGTACTACCT GAAAAGCAAC
    ATGGAGACAG AGACACTGTG CTCGGATGAA GATGCGCAGG AGTTGCTACG AGAGAGCCAT
    ATCAGCCTGC TGCAGCTCAG CACGGTGGAA GTGGCAACCC AACTCTCCAT GCGCGCCTTT
    GAGCTGTTCT GTGCCATCGA GCCCACCGAG TACATCGATG ACCTGTTTAA GCTGAAAACT
    CGCCTCACCG GACCCCCCAG CCTCAAGCTT TTTGAGGAGG CCATCAATCG GGAGACTTTC
    TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC AACCAGCTCA AACGCATGAA GATTGTCAAG
    CACTTCATTA AGATTGCACT GCACTGCCGC GAATGCAAGA ACTTCAACTC CATGTTTGCC
    ATCATCAGTG GTCTGAACTT GGCACCAGTG TCCAGGCTGA GAGGCACATG GGAGAAGCTG
    CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT CTTCAAGACC TCTTCGATCC CTCCAGGAAT
    ATGGCCAAGT ATCGCAACCT TCTAAACAGC CAGAACCTGC AGCCCCCTAT CATCCCACTC
    TTCCCCGTAA TCAAGAAGGA TCTCACTTTC CTGCATGAAG GGAATGACTC CAAAGTGGAC
    GGTTTGGTGA ACTTTGAGAA GCTGCGGATG ATCGCTAAAG AGATCCGACA CGTGGGCAGA
    ATGGCAGCCG TAAACATGGA CCCTGCACTG ATGTTCAGAA CCAGGAAGAA GAAATGGAGG
    AGTTTAGGGT CACTCAGCCA GGGCAGTGCC AACGCGGCTG TCCTGGATGT GACCCAGACG
    GGTGGGCACA AGAAGCGGGT CAGAAGGAGT TCCTTCCTTA ATGCCAAGAA GCTGTACGAG
    GACGCACAGA TGGCACGCAA AGTCAAACAA TACCTGTCCA ACCTCACCCT GGAGACCAAC
    GAGGAGAGCC TGCAAACGCT CTCAATGCAG TGTGAGCCCT CAATCAACAC CTTGCCGAAG
    AGTTCTGGGG ACAGAAAGAG ACCCGACACA TCACCCGTAG TCTCACGGGC TGCGACCCAC
    CAGCGCCAGC AGCTTCAAAA AGCCAACCAG GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC
    CCATCCCGCA AAAAAGTGCC AGTCAAAGAC TTACCGCCAT TTGGCACAAG CTCACCACAG
    TCTCTGAAGA AGATCCTGTC CCTTTCAGAG GAGGCAGGAG ACAGACACCG AAAGCAGGCA
    GAGGACAGTG TGTCCAACAA CTCCTCACAG CTCTCGTCTC CTCCAACATC TCCTCACAGC
    TCACCCAAAA AAGCCATCGC ATCAATAAGA AGTCATGGTA TGAAGGGCTT TTCCTCCTTG
    TGTAGTAAAT CACCAACAGG TTATGGACGA GCTGGTGATT ACCTGTCCGA CTCTGGCCAC
    AGTGAGATCT CCTCCCGCTC CAGTCTGGTG AGCACTTCTT CTCTGGACAT GGGGCAGGAC
    GAGCGCCGGC AGCGTTACGG AGGAACGGCC GGAGAAGGAC ACGGCAGTGG CCACCGGCTC
    GAGCGCAGAG CTACTGCTGA CCCTGACCAG TACAGCTTGG GGTCCTACTC CTCCATGCAG
    GACTGTCGTG GTCTGTACGC AGGCGCCACC GTCCTGTCCT CACCCAGCTC AGAGGAGCTC
    ACGCAGGACC AGGGTGACCG GGTGTCCCTG GACGCTGCGG ATTCCGGTCG TGGCAGCTGG
    ACTTCGTGCT CCAGCGGCTC TCATGATAAC ATCCAGACCA TGCAGCAGGG CCGCAGCTGG
    GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG GGCAGCAGAG GGAGCTGGGC ATCCGCATGC
    TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC GAGGACTCTG AAGGCGACAC TGGCACCATC
    AAACGGAGAG GAGGGAGGGA TGTGACCACA GATCCAGAGA CCAGCAGCAT CACCTCCACC
    GGGTCGGAAG AGTCCAGACA GCACAGCCGA CGGCCCTCAC CCATCACCGC AGGAAACACC
    AAGGCTTCCA TCACACGGAA GGACGGCCGC TATCGGGACC CTCCACCAAC GCCGCCGGGT
    TACACCGCGC TGACCATCAC AGATGTGACC GAGGGAACGC CACATTCGGG CAGGAAACCA
    CCAGACTACA CCACCGCCCT GCAGCGCTCG CGCCTCGTCA CACACTCCCT CGACTCCCAG
    CAGCCCCCAG CCGCAGGGAA ACCGGACCCC CGGGGCCGAG AGGAGGACGA GGCAGTGGAG
    GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC AAACTCAGAG CTCAGAGGAG GAGAGGTCCA
    CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55032
    Clone ID Related Accession (Same CDS sequence) XM_017359174.2 , XM_017359174.1
    Accession Version XM_017359174.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGAAGCG GTCCCTATAA ACATGTCAGA CCAGGTATTG
    CGAGTGTTCA AGGCGGACCA GCAAAGTCGG TACATCATGA TTGGGAAGGA CACCACGGCA
    AAAGAAGTGG TGGCTCAGGC CATCCGAGAG TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT
    TCGTTGTGTG AGGTGTCAGT CACTCCCGAA GGAGTCATCA AGCAAAGGAG ACTCCCTGAA
    CAACTCTCCA AACTGGCTGA CAGGATCCAG CTGAGTGGAA GGTACTACCT GAAAAGCAAC
    ATGGAGACAG AGACACTGTG CTCGGATGAA GATGCGCAGG AGTTGCTACG AGAGAGCCAT
    ATCAGCCTGC TGCAGCTCAG CACGGTGGAA GTGGCAACCC AACTCTCCAT GCGCGCCTTT
    GAGCTGTTCT GTGCCATCGA GCCCACCGAG TACATCGATG ACCTGTTTAA GCTGAAAACT
    CGCCTCACCG GACCCCCCAG CCTCAAGCTT TTTGAGGAGG CCATCAATCG GGAGACTTTC
    TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC AACCAGCTCA AACGCATGAA GATTGTCAAG
    CACTTCATTA AGATTGCACT GCACTGCCGC GAATGCAAGA ACTTCAACTC CATGTTTGCC
    ATCATCAGTG GTCTGAACTT GGCACCAGTG TCCAGGCTGA GAGGCACATG GGAGAAGCTG
    CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT CTTCAAGACC TCTTCGATCC CTCCAGGAAT
    ATGGCCAAGT ATCGCAACCT TCTAAACAGC CAGAACCTGC AGCCCCCTAT CATCCCACTC
    TTCCCCGTAA TCAAGAAGGA TCTCACTTTC CTGCATGAAG GGAATGACTC CAAAGTGGAC
    GGTTTGGTGA ACTTTGAGAA GCTGCGGATG ATCGCTAAAG AGATCCGACA CGTGGGCAGA
    ATGGCAGCCG TAAACATGGA CCCTGCACTG ATGTTCAGAA CCAGGAAGAA GAAATGGAGG
    AGTTTAGGGT CACTCAGCCA GGGCAGTGCC AACGCGGCTG TCCTGGATGT GACCCAGACG
    GGTGGGCACA AGAAGCGGGT CAGAAGGAGT TCCTTCCTTA ATGCCAAGAA GCTGTACGAG
    GACGCACAGA TGGCACGCAA AGTCAAACAA TACCTGTCCA ACCTCACCCT GGAGACCAAC
    GAGGAGAGCC TGCAAACGCT CTCAATGCAG TGTGAGCCCT CAATCAACAC CTTGCCGAAG
    AGTTCTGGGG ACAGAAAGAG ACCCGACACA TCACCCGTAG TCTCACGGGC TGCGACCCAC
    CAGCGCCAGC AGCTTCAAAA AGCCAACCAG GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC
    CCATCCCGCA AAAAAGTGCC AGTCAAAGAC TTACCGCCAT TTGGCACAAG CTCACCACAG
    TCTCTGAAGA AGATCCTGTC CCTTTCAGAG GAGGCAGGAG ACAGACACCG AAAGCAGGCA
    GAGGACAGTG TGTCCAACAA CTCCTCACAG CTCTCGTCTC CTCCAACATC TCCTCACAGC
    TCACCCAAAA AAGCCATCGC ATCAATAAGA AGTCATGGTA TGAAGGGCTT TTCCTCCTTG
    TGTAGTAAAT CACCAACAGG TTATGGACGA GCTGGTGATT ACCTGTCCGA CTCTGGCCAC
    AGTGAGATCT CCTCCCGCTC CAGTCTGGTG AGCACTTCTT CTCTGGACAT GGGGCAGGAC
    GAGCGCCGGC AGCGTTACGG AGGAACGGCC GGAGAAGGAC ACGGCAGTGG CCACCGGCTC
    GAGCGCAGAG CTACTGCTGA CCCTGACCAG TACAGCTTGG GGTCCTACTC CTCCATGCAG
    GACTGTCGTG GTCTGTACGC AGGCGCCACC GTCCTGTCCT CACCCAGCTC AGAGGAGCTC
    ACGCAGGACC AGGGTGACCG GGTGTCCCTG GACGCTGCGG ATTCCGGTCG TGGCAGCTGG
    ACTTCGTGCT CCAGCGGCTC TCATGATAAC ATCCAGACCA TGCAGCAGGG CCGCAGCTGG
    GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG GGCAGCAGAG GGAGCTGGGC ATCCGCATGC
    TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC GAGGACTCTG AAGGCGACAC TGGCACCATC
    AAACGGAGAG GAGGGAGGGA TGTGACCACA GATCCAGAGA CCAGCAGCAT CACCTCCACC
    GGGTCGGAAG AGTCCAGACA GCACAGCCGA CGGCCCTCAC CCATCACCGC AGGAAACACC
    AAGGCTTCCA TCACACGGAA GGACGGCCGC TATCGGGACC CTCCACCAAC GCCGCCGGGT
    TACACCGCGC TGACCATCAC AGATGTGACC GAGGGAACGC CACATTCGGG CAGGAAACCA
    CCAGACTACA CCACCGCCCT GCAGCGCTCG CGCCTCGTCA CACACTCCCT CGACTCCCAG
    CAGCCCCCAG CCGCAGGGAA ACCGGACCCC CGGGGCCGAG AGGAGGACGA GGCAGTGGAG
    GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC AAACTCAGAG CTCAGAGGAG GAGAGGTCCA
    CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214663.1
    CDS607..5493
    Translation

    Target ORF information:

    RefSeq Version XM_017359174.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGAAGCG GTCCCTATAA ACATGTCAGA CCAGGTATTG
    CGAGTGTTCA AGGCGGACCA GCAAAGTCGG TACATCATGA TTGGGAAGGA CACCACGGCA
    AAAGAAGTGG TGGCTCAGGC CATCCGAGAG TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT
    TCGTTGTGTG AGGTGTCAGT CACTCCCGAA GGAGTCATCA AGCAAAGGAG ACTCCCTGAA
    CAACTCTCCA AACTGGCTGA CAGGATCCAG CTGAGTGGAA GGTACTACCT GAAAAGCAAC
    ATGGAGACAG AGACACTGTG CTCGGATGAA GATGCGCAGG AGTTGCTACG AGAGAGCCAT
    ATCAGCCTGC TGCAGCTCAG CACGGTGGAA GTGGCAACCC AACTCTCCAT GCGCGCCTTT
    GAGCTGTTCT GTGCCATCGA GCCCACCGAG TACATCGATG ACCTGTTTAA GCTGAAAACT
    CGCCTCACCG GACCCCCCAG CCTCAAGCTT TTTGAGGAGG CCATCAATCG GGAGACTTTC
    TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC AACCAGCTCA AACGCATGAA GATTGTCAAG
    CACTTCATTA AGATTGCACT GCACTGCCGC GAATGCAAGA ACTTCAACTC CATGTTTGCC
    ATCATCAGTG GTCTGAACTT GGCACCAGTG TCCAGGCTGA GAGGCACATG GGAGAAGCTG
    CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT CTTCAAGACC TCTTCGATCC CTCCAGGAAT
    ATGGCCAAGT ATCGCAACCT TCTAAACAGC CAGAACCTGC AGCCCCCTAT CATCCCACTC
    TTCCCCGTAA TCAAGAAGGA TCTCACTTTC CTGCATGAAG GGAATGACTC CAAAGTGGAC
    GGTTTGGTGA ACTTTGAGAA GCTGCGGATG ATCGCTAAAG AGATCCGACA CGTGGGCAGA
    ATGGCAGCCG TAAACATGGA CCCTGCACTG ATGTTCAGAA CCAGGAAGAA GAAATGGAGG
    AGTTTAGGGT CACTCAGCCA GGGCAGTGCC AACGCGGCTG TCCTGGATGT GACCCAGACG
    GGTGGGCACA AGAAGCGGGT CAGAAGGAGT TCCTTCCTTA ATGCCAAGAA GCTGTACGAG
    GACGCACAGA TGGCACGCAA AGTCAAACAA TACCTGTCCA ACCTCACCCT GGAGACCAAC
    GAGGAGAGCC TGCAAACGCT CTCAATGCAG TGTGAGCCCT CAATCAACAC CTTGCCGAAG
    AGTTCTGGGG ACAGAAAGAG ACCCGACACA TCACCCGTAG TCTCACGGGC TGCGACCCAC
    CAGCGCCAGC AGCTTCAAAA AGCCAACCAG GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC
    CCATCCCGCA AAAAAGTGCC AGTCAAAGAC TTACCGCCAT TTGGCACAAG CTCACCACAG
    TCTCTGAAGA AGATCCTGTC CCTTTCAGAG GAGGCAGGAG ACAGACACCG AAAGCAGGCA
    GAGGACAGTG TGTCCAACAA CTCCTCACAG CTCTCGTCTC CTCCAACATC TCCTCACAGC
    TCACCCAAAA AAGCCATCGC ATCAATAAGA AGTCATGGTA TGAAGGGCTT TTCCTCCTTG
    TGTAGTAAAT CACCAACAGG TTATGGACGA GCTGGTGATT ACCTGTCCGA CTCTGGCCAC
    AGTGAGATCT CCTCCCGCTC CAGTCTGGTG AGCACTTCTT CTCTGGACAT GGGGCAGGAC
    GAGCGCCGGC AGCGTTACGG AGGAACGGCC GGAGAAGGAC ACGGCAGTGG CCACCGGCTC
    GAGCGCAGAG CTACTGCTGA CCCTGACCAG TACAGCTTGG GGTCCTACTC CTCCATGCAG
    GACTGTCGTG GTCTGTACGC AGGCGCCACC GTCCTGTCCT CACCCAGCTC AGAGGAGCTC
    ACGCAGGACC AGGGTGACCG GGTGTCCCTG GACGCTGCGG ATTCCGGTCG TGGCAGCTGG
    ACTTCGTGCT CCAGCGGCTC TCATGATAAC ATCCAGACCA TGCAGCAGGG CCGCAGCTGG
    GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG GGCAGCAGAG GGAGCTGGGC ATCCGCATGC
    TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC GAGGACTCTG AAGGCGACAC TGGCACCATC
    AAACGGAGAG GAGGGAGGGA TGTGACCACA GATCCAGAGA CCAGCAGCAT CACCTCCACC
    GGGTCGGAAG AGTCCAGACA GCACAGCCGA CGGCCCTCAC CCATCACCGC AGGAAACACC
    AAGGCTTCCA TCACACGGAA GGACGGCCGC TATCGGGACC CTCCACCAAC GCCGCCGGGT
    TACACCGCGC TGACCATCAC AGATGTGACC GAGGGAACGC CACATTCGGG CAGGAAACCA
    CCAGACTACA CCACCGCCCT GCAGCGCTCG CGCCTCGTCA CACACTCCCT CGACTCCCAG
    CAGCCCCCAG CCGCAGGGAA ACCGGACCCC CGGGGCCGAG AGGAGGACGA GGCAGTGGAG
    GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC AAACTCAGAG CTCAGAGGAG GAGAGGTCCA
    CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55033
    Clone ID Related Accession (Same CDS sequence) XM_017359176.2 , XM_017359176.1
    Accession Version XM_017359176.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGCCA TCGCATCAAT AAGAAGTCAT GGTATGAAGG GCTTTTCCTC CTTGTGTAGT
    AAATCACCAA CAGGTTATGG ACGAGCTGGT GATTACCTGT CCGACTCTGG CCACAGTGAG
    ATCTCCTCCC GCTCCAGTCT GGTGAGCACT TCTTCTCTGG ACATGGGGCA GGACGAGCGC
    CGGCAGCGTT ACGGAGGAAC GGCCGGAGAA GGACACGGCA GTGGCCACCG GCTCGAGCGC
    AGAGCTACTG CTGACCCTGA CCAGTACAGC TTGGGGTCCT ACTCCTCCAT GCAGGACTGT
    CGTGGTCTGT ACGCAGGCGC CACCGTCCTG TCCTCACCCA GCTCAGAGGA GCTCACGCAG
    GACCAGGGTG ACCGGGTGTC CCTGGACGCT GCGGATTCCG GTCGTGGCAG CTGGACTTCG
    TGCTCCAGCG GCTCTCATGA TAACATCCAG ACCATGCAGC AGGGCCGCAG CTGGGAGGCG
    CTGGCAGAGG GCGCAGGCCT GTGGGGCAGC AGAGGGAGCT GGGCATCCGC ATGCTCCTCT
    TCTTCTTCGG TGGCATGTTG GGGCGAGGAC TCTGAAGGCG ACACTGGCAC CATCAAACGG
    AGAGGAGGGA GGGATGTGAC CACAGATCCA GAGACCAGCA GCATCACCTC CACCGGGTCG
    GAAGAGTCCA GACAGCACAG CCGACGGCCC TCACCCATCA CCGCAGGAAA CACCAAGGCT
    TCCATCACAC GGAAGGACGG CCGCTATCGG GACCCTCCAC CAACGCCGCC GGGTTACACC
    GCGCTGACCA TCACAGATGT GACCGAGGGA ACGCCACATT CGGGCAGGAA ACCACCAGAC
    TACACCACCG CCCTGCAGCG CTCGCGCCTC GTCACACACT CCCTCGACTC CCAGCAGCCC
    CCAGCCGCAG GGAAACCGGA CCCCCGGGGC CGAGAGGAGG ACGAGGCAGT GGAGGTGGAG
    GAGGAGGGTG AGTGCTTGTC TCCCAAACTC AGAGCTCAGA GGAGGAGAGG TCCACACACA
    GCAGTTCCCC CCAGGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214665.1
    CDS543..5423
    Translation

    Target ORF information:

    RefSeq Version XM_017359176.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359176.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGCCA TCGCATCAAT AAGAAGTCAT GGTATGAAGG GCTTTTCCTC CTTGTGTAGT
    AAATCACCAA CAGGTTATGG ACGAGCTGGT GATTACCTGT CCGACTCTGG CCACAGTGAG
    ATCTCCTCCC GCTCCAGTCT GGTGAGCACT TCTTCTCTGG ACATGGGGCA GGACGAGCGC
    CGGCAGCGTT ACGGAGGAAC GGCCGGAGAA GGACACGGCA GTGGCCACCG GCTCGAGCGC
    AGAGCTACTG CTGACCCTGA CCAGTACAGC TTGGGGTCCT ACTCCTCCAT GCAGGACTGT
    CGTGGTCTGT ACGCAGGCGC CACCGTCCTG TCCTCACCCA GCTCAGAGGA GCTCACGCAG
    GACCAGGGTG ACCGGGTGTC CCTGGACGCT GCGGATTCCG GTCGTGGCAG CTGGACTTCG
    TGCTCCAGCG GCTCTCATGA TAACATCCAG ACCATGCAGC AGGGCCGCAG CTGGGAGGCG
    CTGGCAGAGG GCGCAGGCCT GTGGGGCAGC AGAGGGAGCT GGGCATCCGC ATGCTCCTCT
    TCTTCTTCGG TGGCATGTTG GGGCGAGGAC TCTGAAGGCG ACACTGGCAC CATCAAACGG
    AGAGGAGGGA GGGATGTGAC CACAGATCCA GAGACCAGCA GCATCACCTC CACCGGGTCG
    GAAGAGTCCA GACAGCACAG CCGACGGCCC TCACCCATCA CCGCAGGAAA CACCAAGGCT
    TCCATCACAC GGAAGGACGG CCGCTATCGG GACCCTCCAC CAACGCCGCC GGGTTACACC
    GCGCTGACCA TCACAGATGT GACCGAGGGA ACGCCACATT CGGGCAGGAA ACCACCAGAC
    TACACCACCG CCCTGCAGCG CTCGCGCCTC GTCACACACT CCCTCGACTC CCAGCAGCCC
    CCAGCCGCAG GGAAACCGGA CCCCCGGGGC CGAGAGGAGG ACGAGGCAGT GGAGGTGGAG
    GAGGAGGGTG AGTGCTTGTC TCCCAAACTC AGAGCTCAGA GGAGGAGAGG TCCACACACA
    GCAGTTCCCC CCAGGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55033
    Clone ID Related Accession (Same CDS sequence) XM_017359176.2 , XM_017359176.1
    Accession Version XM_017359176.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGCCA TCGCATCAAT AAGAAGTCAT GGTATGAAGG GCTTTTCCTC CTTGTGTAGT
    AAATCACCAA CAGGTTATGG ACGAGCTGGT GATTACCTGT CCGACTCTGG CCACAGTGAG
    ATCTCCTCCC GCTCCAGTCT GGTGAGCACT TCTTCTCTGG ACATGGGGCA GGACGAGCGC
    CGGCAGCGTT ACGGAGGAAC GGCCGGAGAA GGACACGGCA GTGGCCACCG GCTCGAGCGC
    AGAGCTACTG CTGACCCTGA CCAGTACAGC TTGGGGTCCT ACTCCTCCAT GCAGGACTGT
    CGTGGTCTGT ACGCAGGCGC CACCGTCCTG TCCTCACCCA GCTCAGAGGA GCTCACGCAG
    GACCAGGGTG ACCGGGTGTC CCTGGACGCT GCGGATTCCG GTCGTGGCAG CTGGACTTCG
    TGCTCCAGCG GCTCTCATGA TAACATCCAG ACCATGCAGC AGGGCCGCAG CTGGGAGGCG
    CTGGCAGAGG GCGCAGGCCT GTGGGGCAGC AGAGGGAGCT GGGCATCCGC ATGCTCCTCT
    TCTTCTTCGG TGGCATGTTG GGGCGAGGAC TCTGAAGGCG ACACTGGCAC CATCAAACGG
    AGAGGAGGGA GGGATGTGAC CACAGATCCA GAGACCAGCA GCATCACCTC CACCGGGTCG
    GAAGAGTCCA GACAGCACAG CCGACGGCCC TCACCCATCA CCGCAGGAAA CACCAAGGCT
    TCCATCACAC GGAAGGACGG CCGCTATCGG GACCCTCCAC CAACGCCGCC GGGTTACACC
    GCGCTGACCA TCACAGATGT GACCGAGGGA ACGCCACATT CGGGCAGGAA ACCACCAGAC
    TACACCACCG CCCTGCAGCG CTCGCGCCTC GTCACACACT CCCTCGACTC CCAGCAGCCC
    CCAGCCGCAG GGAAACCGGA CCCCCGGGGC CGAGAGGAGG ACGAGGCAGT GGAGGTGGAG
    GAGGAGGGTG AGTGCTTGTC TCCCAAACTC AGAGCTCAGA GGAGGAGAGG TCCACACACA
    GCAGTTCCCC CCAGGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214665.1
    CDS607..5487
    Translation

    Target ORF information:

    RefSeq Version XM_017359176.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGCCA TCGCATCAAT AAGAAGTCAT GGTATGAAGG GCTTTTCCTC CTTGTGTAGT
    AAATCACCAA CAGGTTATGG ACGAGCTGGT GATTACCTGT CCGACTCTGG CCACAGTGAG
    ATCTCCTCCC GCTCCAGTCT GGTGAGCACT TCTTCTCTGG ACATGGGGCA GGACGAGCGC
    CGGCAGCGTT ACGGAGGAAC GGCCGGAGAA GGACACGGCA GTGGCCACCG GCTCGAGCGC
    AGAGCTACTG CTGACCCTGA CCAGTACAGC TTGGGGTCCT ACTCCTCCAT GCAGGACTGT
    CGTGGTCTGT ACGCAGGCGC CACCGTCCTG TCCTCACCCA GCTCAGAGGA GCTCACGCAG
    GACCAGGGTG ACCGGGTGTC CCTGGACGCT GCGGATTCCG GTCGTGGCAG CTGGACTTCG
    TGCTCCAGCG GCTCTCATGA TAACATCCAG ACCATGCAGC AGGGCCGCAG CTGGGAGGCG
    CTGGCAGAGG GCGCAGGCCT GTGGGGCAGC AGAGGGAGCT GGGCATCCGC ATGCTCCTCT
    TCTTCTTCGG TGGCATGTTG GGGCGAGGAC TCTGAAGGCG ACACTGGCAC CATCAAACGG
    AGAGGAGGGA GGGATGTGAC CACAGATCCA GAGACCAGCA GCATCACCTC CACCGGGTCG
    GAAGAGTCCA GACAGCACAG CCGACGGCCC TCACCCATCA CCGCAGGAAA CACCAAGGCT
    TCCATCACAC GGAAGGACGG CCGCTATCGG GACCCTCCAC CAACGCCGCC GGGTTACACC
    GCGCTGACCA TCACAGATGT GACCGAGGGA ACGCCACATT CGGGCAGGAA ACCACCAGAC
    TACACCACCG CCCTGCAGCG CTCGCGCCTC GTCACACACT CCCTCGACTC CCAGCAGCCC
    CCAGCCGCAG GGAAACCGGA CCCCCGGGGC CGAGAGGAGG ACGAGGCAGT GGAGGTGGAG
    GAGGAGGGTG AGTGCTTGTC TCCCAAACTC AGAGCTCAGA GGAGGAGAGG TCCACACACA
    GCAGTTCCCC CCAGGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55034
    Clone ID Related Accession (Same CDS sequence) XM_017359177.1 , XM_017359177.2
    Accession Version XM_017359177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GGTTATGGAC GAGCTGGTGA TTACCTGTCC
    GACTCTGGCC ACAGTGAGAT CTCCTCCCGC TCCAGTCTGG TGAGCACTTC TTCTCTGGAC
    ATGGGGCAGG ACGAGCGCCG GCAGCGTTAC GGAGGAACGG CCGGAGAAGG ACACGGCAGT
    GGCCACCGGC TCGAGCGCAG AGCTACTGCT GACCCTGACC AGTACAGCTT GGGGTCCTAC
    TCCTCCATGC AGGACTGTCG TGGTCTGTAC GCAGGCGCCA CCGTCCTGTC CTCACCCAGC
    TCAGAGGAGC TCACGCAGGA CCAGGGTGAC CGGGTGTCCC TGGACGCTGC GGATTCCGGT
    CGTGGCAGCT GGACTTCGTG CTCCAGCGGC TCTCATGATA ACATCCAGAC CATGCAGCAG
    GGCCGCAGCT GGGAGGCGCT GGCAGAGGGC GCAGGCCTGT GGGGCAGCAG AGGGAGCTGG
    GCATCCGCAT GCTCCTCTTC TTCTTCGGTG GCATGTTGGG GCGAGGACTC TGAAGGCGAC
    ACTGGCACCA TCAAACGGAG AGGAGGGAGG GATGTGACCA CAGATCCAGA GACCAGCAGC
    ATCACCTCCA CCGGGTCGGA AGAGTCCAGA CAGCACAGCC GACGGCCCTC ACCCATCACC
    GCAGGAAACA CCAAGGCTTC CATCACACGG AAGGACGGCC GCTATCGGGA CCCTCCACCA
    ACGCCGCCGG GTTACACCGC GCTGACCATC ACAGATGTGA CCGAGGGAAC GCCACATTCG
    GGCAGGAAAC CACCAGACTA CACCACCGCC CTGCAGCGCT CGCGCCTCGT CACACACTCC
    CTCGACTCCC AGCAGCCCCC AGCCGCAGGG AAACCGGACC CCCGGGGCCG AGAGGAGGAC
    GAGGCAGTGG AGGTGGAGGA GGAGGGTGAG TGCTTGTCTC CCAAACTCAG AGCTCAGAGG
    AGGAGAGGTC CACACACAGC AGTTCCCCCC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214666.1
    CDS606..5444
    Translation

    Target ORF information:

    RefSeq Version XM_017359177.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GGTTATGGAC GAGCTGGTGA TTACCTGTCC
    GACTCTGGCC ACAGTGAGAT CTCCTCCCGC TCCAGTCTGG TGAGCACTTC TTCTCTGGAC
    ATGGGGCAGG ACGAGCGCCG GCAGCGTTAC GGAGGAACGG CCGGAGAAGG ACACGGCAGT
    GGCCACCGGC TCGAGCGCAG AGCTACTGCT GACCCTGACC AGTACAGCTT GGGGTCCTAC
    TCCTCCATGC AGGACTGTCG TGGTCTGTAC GCAGGCGCCA CCGTCCTGTC CTCACCCAGC
    TCAGAGGAGC TCACGCAGGA CCAGGGTGAC CGGGTGTCCC TGGACGCTGC GGATTCCGGT
    CGTGGCAGCT GGACTTCGTG CTCCAGCGGC TCTCATGATA ACATCCAGAC CATGCAGCAG
    GGCCGCAGCT GGGAGGCGCT GGCAGAGGGC GCAGGCCTGT GGGGCAGCAG AGGGAGCTGG
    GCATCCGCAT GCTCCTCTTC TTCTTCGGTG GCATGTTGGG GCGAGGACTC TGAAGGCGAC
    ACTGGCACCA TCAAACGGAG AGGAGGGAGG GATGTGACCA CAGATCCAGA GACCAGCAGC
    ATCACCTCCA CCGGGTCGGA AGAGTCCAGA CAGCACAGCC GACGGCCCTC ACCCATCACC
    GCAGGAAACA CCAAGGCTTC CATCACACGG AAGGACGGCC GCTATCGGGA CCCTCCACCA
    ACGCCGCCGG GTTACACCGC GCTGACCATC ACAGATGTGA CCGAGGGAAC GCCACATTCG
    GGCAGGAAAC CACCAGACTA CACCACCGCC CTGCAGCGCT CGCGCCTCGT CACACACTCC
    CTCGACTCCC AGCAGCCCCC AGCCGCAGGG AAACCGGACC CCCGGGGCCG AGAGGAGGAC
    GAGGCAGTGG AGGTGGAGGA GGAGGGTGAG TGCTTGTCTC CCAAACTCAG AGCTCAGAGG
    AGGAGAGGTC CACACACAGC AGTTCCCCCC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55034
    Clone ID Related Accession (Same CDS sequence) XM_017359177.1 , XM_017359177.2
    Accession Version XM_017359177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GGTTATGGAC GAGCTGGTGA TTACCTGTCC
    GACTCTGGCC ACAGTGAGAT CTCCTCCCGC TCCAGTCTGG TGAGCACTTC TTCTCTGGAC
    ATGGGGCAGG ACGAGCGCCG GCAGCGTTAC GGAGGAACGG CCGGAGAAGG ACACGGCAGT
    GGCCACCGGC TCGAGCGCAG AGCTACTGCT GACCCTGACC AGTACAGCTT GGGGTCCTAC
    TCCTCCATGC AGGACTGTCG TGGTCTGTAC GCAGGCGCCA CCGTCCTGTC CTCACCCAGC
    TCAGAGGAGC TCACGCAGGA CCAGGGTGAC CGGGTGTCCC TGGACGCTGC GGATTCCGGT
    CGTGGCAGCT GGACTTCGTG CTCCAGCGGC TCTCATGATA ACATCCAGAC CATGCAGCAG
    GGCCGCAGCT GGGAGGCGCT GGCAGAGGGC GCAGGCCTGT GGGGCAGCAG AGGGAGCTGG
    GCATCCGCAT GCTCCTCTTC TTCTTCGGTG GCATGTTGGG GCGAGGACTC TGAAGGCGAC
    ACTGGCACCA TCAAACGGAG AGGAGGGAGG GATGTGACCA CAGATCCAGA GACCAGCAGC
    ATCACCTCCA CCGGGTCGGA AGAGTCCAGA CAGCACAGCC GACGGCCCTC ACCCATCACC
    GCAGGAAACA CCAAGGCTTC CATCACACGG AAGGACGGCC GCTATCGGGA CCCTCCACCA
    ACGCCGCCGG GTTACACCGC GCTGACCATC ACAGATGTGA CCGAGGGAAC GCCACATTCG
    GGCAGGAAAC CACCAGACTA CACCACCGCC CTGCAGCGCT CGCGCCTCGT CACACACTCC
    CTCGACTCCC AGCAGCCCCC AGCCGCAGGG AAACCGGACC CCCGGGGCCG AGAGGAGGAC
    GAGGCAGTGG AGGTGGAGGA GGAGGGTGAG TGCTTGTCTC CCAAACTCAG AGCTCAGAGG
    AGGAGAGGTC CACACACAGC AGTTCCCCCC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214666.1
    CDS542..5380
    Translation

    Target ORF information:

    RefSeq Version XM_017359177.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GGTTATGGAC GAGCTGGTGA TTACCTGTCC
    GACTCTGGCC ACAGTGAGAT CTCCTCCCGC TCCAGTCTGG TGAGCACTTC TTCTCTGGAC
    ATGGGGCAGG ACGAGCGCCG GCAGCGTTAC GGAGGAACGG CCGGAGAAGG ACACGGCAGT
    GGCCACCGGC TCGAGCGCAG AGCTACTGCT GACCCTGACC AGTACAGCTT GGGGTCCTAC
    TCCTCCATGC AGGACTGTCG TGGTCTGTAC GCAGGCGCCA CCGTCCTGTC CTCACCCAGC
    TCAGAGGAGC TCACGCAGGA CCAGGGTGAC CGGGTGTCCC TGGACGCTGC GGATTCCGGT
    CGTGGCAGCT GGACTTCGTG CTCCAGCGGC TCTCATGATA ACATCCAGAC CATGCAGCAG
    GGCCGCAGCT GGGAGGCGCT GGCAGAGGGC GCAGGCCTGT GGGGCAGCAG AGGGAGCTGG
    GCATCCGCAT GCTCCTCTTC TTCTTCGGTG GCATGTTGGG GCGAGGACTC TGAAGGCGAC
    ACTGGCACCA TCAAACGGAG AGGAGGGAGG GATGTGACCA CAGATCCAGA GACCAGCAGC
    ATCACCTCCA CCGGGTCGGA AGAGTCCAGA CAGCACAGCC GACGGCCCTC ACCCATCACC
    GCAGGAAACA CCAAGGCTTC CATCACACGG AAGGACGGCC GCTATCGGGA CCCTCCACCA
    ACGCCGCCGG GTTACACCGC GCTGACCATC ACAGATGTGA CCGAGGGAAC GCCACATTCG
    GGCAGGAAAC CACCAGACTA CACCACCGCC CTGCAGCGCT CGCGCCTCGT CACACACTCC
    CTCGACTCCC AGCAGCCCCC AGCCGCAGGG AAACCGGACC CCCGGGGCCG AGAGGAGGAC
    GAGGCAGTGG AGGTGGAGGA GGAGGGTGAG TGCTTGTCTC CCAAACTCAG AGCTCAGAGG
    AGGAGAGGTC CACACACAGC AGTTCCCCCC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55035
    Clone ID Related Accession (Same CDS sequence) XM_017359178.2 , XM_017359178.1
    Accession Version XM_017359178.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGGTTA TGGACGAGCT GGTGATTACC TGTCCGACTC TGGCCACAGT
    GAGATCTCCT CCCGCTCCAG TCTGGTGAGC ACTTCTTCTC TGGACATGGG GCAGGACGAG
    CGCCGGCAGC GTTACGGAGG AACGGCCGGA GAAGGACACG GCAGTGGCCA CCGGCTCGAG
    CGCAGAGCTA CTGCTGACCC TGACCAGTAC AGCTTGGGGT CCTACTCCTC CATGCAGGAC
    TGTCGTGGTC TGTACGCAGG CGCCACCGTC CTGTCCTCAC CCAGCTCAGA GGAGCTCACG
    CAGGACCAGG GTGACCGGGT GTCCCTGGAC GCTGCGGATT CCGGTCGTGG CAGCTGGACT
    TCGTGCTCCA GCGGCTCTCA TGATAACATC CAGACCATGC AGCAGGGCCG CAGCTGGGAG
    GCGCTGGCAG AGGGCGCAGG CCTGTGGGGC AGCAGAGGGA GCTGGGCATC CGCATGCTCC
    TCTTCTTCTT CGGTGGCATG TTGGGGCGAG GACTCTGAAG GCGACACTGG CACCATCAAA
    CGGAGAGGAG GGAGGGATGT GACCACAGAT CCAGAGACCA GCAGCATCAC CTCCACCGGG
    TCGGAAGAGT CCAGACAGCA CAGCCGACGG CCCTCACCCA TCACCGCAGG AAACACCAAG
    GCTTCCATCA CACGGAAGGA CGGCCGCTAT CGGGACCCTC CACCAACGCC GCCGGGTTAC
    ACCGCGCTGA CCATCACAGA TGTGACCGAG GGAACGCCAC ATTCGGGCAG GAAACCACCA
    GACTACACCA CCGCCCTGCA GCGCTCGCGC CTCGTCACAC ACTCCCTCGA CTCCCAGCAG
    CCCCCAGCCG CAGGGAAACC GGACCCCCGG GGCCGAGAGG AGGACGAGGC AGTGGAGGTG
    GAGGAGGAGG GTGAGTGCTT GTCTCCCAAA CTCAGAGCTC AGAGGAGGAG AGGTCCACAC
    ACAGCAGTTC CCCCCAGGCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214667.1
    CDS541..5364
    Translation

    Target ORF information:

    RefSeq Version XM_017359178.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359178.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGGTTA TGGACGAGCT GGTGATTACC TGTCCGACTC TGGCCACAGT
    GAGATCTCCT CCCGCTCCAG TCTGGTGAGC ACTTCTTCTC TGGACATGGG GCAGGACGAG
    CGCCGGCAGC GTTACGGAGG AACGGCCGGA GAAGGACACG GCAGTGGCCA CCGGCTCGAG
    CGCAGAGCTA CTGCTGACCC TGACCAGTAC AGCTTGGGGT CCTACTCCTC CATGCAGGAC
    TGTCGTGGTC TGTACGCAGG CGCCACCGTC CTGTCCTCAC CCAGCTCAGA GGAGCTCACG
    CAGGACCAGG GTGACCGGGT GTCCCTGGAC GCTGCGGATT CCGGTCGTGG CAGCTGGACT
    TCGTGCTCCA GCGGCTCTCA TGATAACATC CAGACCATGC AGCAGGGCCG CAGCTGGGAG
    GCGCTGGCAG AGGGCGCAGG CCTGTGGGGC AGCAGAGGGA GCTGGGCATC CGCATGCTCC
    TCTTCTTCTT CGGTGGCATG TTGGGGCGAG GACTCTGAAG GCGACACTGG CACCATCAAA
    CGGAGAGGAG GGAGGGATGT GACCACAGAT CCAGAGACCA GCAGCATCAC CTCCACCGGG
    TCGGAAGAGT CCAGACAGCA CAGCCGACGG CCCTCACCCA TCACCGCAGG AAACACCAAG
    GCTTCCATCA CACGGAAGGA CGGCCGCTAT CGGGACCCTC CACCAACGCC GCCGGGTTAC
    ACCGCGCTGA CCATCACAGA TGTGACCGAG GGAACGCCAC ATTCGGGCAG GAAACCACCA
    GACTACACCA CCGCCCTGCA GCGCTCGCGC CTCGTCACAC ACTCCCTCGA CTCCCAGCAG
    CCCCCAGCCG CAGGGAAACC GGACCCCCGG GGCCGAGAGG AGGACGAGGC AGTGGAGGTG
    GAGGAGGAGG GTGAGTGCTT GTCTCCCAAA CTCAGAGCTC AGAGGAGGAG AGGTCCACAC
    ACAGCAGTTC CCCCCAGGCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55035
    Clone ID Related Accession (Same CDS sequence) XM_017359178.2 , XM_017359178.1
    Accession Version XM_017359178.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGGTTA TGGACGAGCT GGTGATTACC TGTCCGACTC TGGCCACAGT
    GAGATCTCCT CCCGCTCCAG TCTGGTGAGC ACTTCTTCTC TGGACATGGG GCAGGACGAG
    CGCCGGCAGC GTTACGGAGG AACGGCCGGA GAAGGACACG GCAGTGGCCA CCGGCTCGAG
    CGCAGAGCTA CTGCTGACCC TGACCAGTAC AGCTTGGGGT CCTACTCCTC CATGCAGGAC
    TGTCGTGGTC TGTACGCAGG CGCCACCGTC CTGTCCTCAC CCAGCTCAGA GGAGCTCACG
    CAGGACCAGG GTGACCGGGT GTCCCTGGAC GCTGCGGATT CCGGTCGTGG CAGCTGGACT
    TCGTGCTCCA GCGGCTCTCA TGATAACATC CAGACCATGC AGCAGGGCCG CAGCTGGGAG
    GCGCTGGCAG AGGGCGCAGG CCTGTGGGGC AGCAGAGGGA GCTGGGCATC CGCATGCTCC
    TCTTCTTCTT CGGTGGCATG TTGGGGCGAG GACTCTGAAG GCGACACTGG CACCATCAAA
    CGGAGAGGAG GGAGGGATGT GACCACAGAT CCAGAGACCA GCAGCATCAC CTCCACCGGG
    TCGGAAGAGT CCAGACAGCA CAGCCGACGG CCCTCACCCA TCACCGCAGG AAACACCAAG
    GCTTCCATCA CACGGAAGGA CGGCCGCTAT CGGGACCCTC CACCAACGCC GCCGGGTTAC
    ACCGCGCTGA CCATCACAGA TGTGACCGAG GGAACGCCAC ATTCGGGCAG GAAACCACCA
    GACTACACCA CCGCCCTGCA GCGCTCGCGC CTCGTCACAC ACTCCCTCGA CTCCCAGCAG
    CCCCCAGCCG CAGGGAAACC GGACCCCCGG GGCCGAGAGG AGGACGAGGC AGTGGAGGTG
    GAGGAGGAGG GTGAGTGCTT GTCTCCCAAA CTCAGAGCTC AGAGGAGGAG AGGTCCACAC
    ACAGCAGTTC CCCCCAGGCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214667.1
    CDS605..5428
    Translation

    Target ORF information:

    RefSeq Version XM_017359178.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGACATGTC AGACCAGGTA
    TTGCGAGTGT TCAAGGCGGA CCAGCAAAGT CGGTACATCA TGATTGGGAA GGACACCACG
    GCAAAAGAAG TGGTGGCTCA GGCCATCCGA GAGTTCGCCC TCACCGCTGC TGCCGAAGCC
    TATTCGTTGT GTGAGGTGTC AGTCACTCCC GAAGGAGTCA TCAAGCAAAG GAGACTCCCT
    GAACAACTCT CCAAACTGGC TGACAGGATC CAGCTGAGTG GAAGGTACTA CCTGAAAAGC
    AACATGGAGA CAGAGACACT GTGCTCGGAT GAAGATGCGC AGGAGTTGCT ACGAGAGAGC
    CATATCAGCC TGCTGCAGCT CAGCACGGTG GAAGTGGCAA CCCAACTCTC CATGCGCGCC
    TTTGAGCTGT TCTGTGCCAT CGAGCCCACC GAGTACATCG ATGACCTGTT TAAGCTGAAA
    ACTCGCCTCA CCGGACCCCC CAGCCTCAAG CTTTTTGAGG AGGCCATCAA TCGGGAGACT
    TTCTGGGTGG CCACAGAGGT GGTGCGAGAG GCCAACCAGC TCAAACGCAT GAAGATTGTC
    AAGCACTTCA TTAAGATTGC ACTGCACTGC CGCGAATGCA AGAACTTCAA CTCCATGTTT
    GCCATCATCA GTGGTCTGAA CTTGGCACCA GTGTCCAGGC TGAGAGGCAC ATGGGAGAAG
    CTGCCCAGCA AATACGAGAA GCTGTTTAGC GATCTTCAAG ACCTCTTCGA TCCCTCCAGG
    AATATGGCCA AGTATCGCAA CCTTCTAAAC AGCCAGAACC TGCAGCCCCC TATCATCCCA
    CTCTTCCCCG TAATCAAGAA GGATCTCACT TTCCTGCATG AAGGGAATGA CTCCAAAGTG
    GACGGTTTGG TGAACTTTGA GAAGCTGCGG ATGATCGCTA AAGAGATCCG ACACGTGGGC
    AGAATGGCAG CCGTAAACAT GGACCCTGCA CTGATGTTCA GAACCAGGAA GAAGAAATGG
    AGGAGTTTAG GGTCACTCAG CCAGGGCAGT GCCAACGCGG CTGTCCTGGA TGTGACCCAG
    ACGGGTGGGC ACAAGAAGCG GGTCAGAAGG AGTTCCTTCC TTAATGCCAA GAAGCTGTAC
    GAGGACGCAC AGATGGCACG CAAAGTCAAA CAATACCTGT CCAACCTCAC CCTGGAGACC
    AACGAGGAGA GCCTGCAAAC GCTCTCAATG CAGTGTGAGC CCTCAATCAA CACCTTGCCG
    AAGAGTTCTG GGGACAGAAA GAGACCCGAC ACATCACCCG TAGTCTCACG GGCTGCGACC
    CACCAGCGCC AGCAGCTTCA AAAAGCCAAC CAGGCGCTTC AAGTGCCTGC CGTAGCCCTC
    TACCCATCCC GCAAAAAAGT GCCAGTCAAA GACTTACCGC CATTTGGCAC AAGCTCACCA
    CAGTCTCTGA AGAAGATCCT GTCCCTTTCA GAGGAGGCAG GAGACAGACA CCGAAAGCAG
    GCAGAGGACA GTGTGTCCAA CAACTCCTCA CAGCTCTCGT CTCCTCCAAC ATCTCCTCAC
    AGCTCACCCA AAAAAGGTTA TGGACGAGCT GGTGATTACC TGTCCGACTC TGGCCACAGT
    GAGATCTCCT CCCGCTCCAG TCTGGTGAGC ACTTCTTCTC TGGACATGGG GCAGGACGAG
    CGCCGGCAGC GTTACGGAGG AACGGCCGGA GAAGGACACG GCAGTGGCCA CCGGCTCGAG
    CGCAGAGCTA CTGCTGACCC TGACCAGTAC AGCTTGGGGT CCTACTCCTC CATGCAGGAC
    TGTCGTGGTC TGTACGCAGG CGCCACCGTC CTGTCCTCAC CCAGCTCAGA GGAGCTCACG
    CAGGACCAGG GTGACCGGGT GTCCCTGGAC GCTGCGGATT CCGGTCGTGG CAGCTGGACT
    TCGTGCTCCA GCGGCTCTCA TGATAACATC CAGACCATGC AGCAGGGCCG CAGCTGGGAG
    GCGCTGGCAG AGGGCGCAGG CCTGTGGGGC AGCAGAGGGA GCTGGGCATC CGCATGCTCC
    TCTTCTTCTT CGGTGGCATG TTGGGGCGAG GACTCTGAAG GCGACACTGG CACCATCAAA
    CGGAGAGGAG GGAGGGATGT GACCACAGAT CCAGAGACCA GCAGCATCAC CTCCACCGGG
    TCGGAAGAGT CCAGACAGCA CAGCCGACGG CCCTCACCCA TCACCGCAGG AAACACCAAG
    GCTTCCATCA CACGGAAGGA CGGCCGCTAT CGGGACCCTC CACCAACGCC GCCGGGTTAC
    ACCGCGCTGA CCATCACAGA TGTGACCGAG GGAACGCCAC ATTCGGGCAG GAAACCACCA
    GACTACACCA CCGCCCTGCA GCGCTCGCGC CTCGTCACAC ACTCCCTCGA CTCCCAGCAG
    CCCCCAGCCG CAGGGAAACC GGACCCCCGG GGCCGAGAGG AGGACGAGGC AGTGGAGGTG
    GAGGAGGAGG GTGAGTGCTT GTCTCCCAAA CTCAGAGCTC AGAGGAGGAG AGGTCCACAC
    ACAGCAGTTC CCCCCAGGCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55036
    Clone ID Related Accession (Same CDS sequence) XM_017359179.1 , XM_017359179.2
    Accession Version XM_017359179.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGGTT ATGGACGAGC TGGTGATTAC CTGTCCGACT CTGGCCACAG TGAGATCTCC
    TCCCGCTCCA GTCTGGTGAG CACTTCTTCT CTGGACATGG GGCAGGACGA GCGCCGGCAG
    CGTTACGGAG GAACGGCCGG AGAAGGACAC GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT
    ACTGCTGACC CTGACCAGTA CAGCTTGGGG TCCTACTCCT CCATGCAGGA CTGTCGTGGT
    CTGTACGCAG GCGCCACCGT CCTGTCCTCA CCCAGCTCAG AGGAGCTCAC GCAGGACCAG
    GGTGACCGGG TGTCCCTGGA CGCTGCGGAT TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC
    AGCGGCTCTC ATGATAACAT CCAGACCATG CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA
    GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG AGCTGGGCAT CCGCATGCTC CTCTTCTTCT
    TCGGTGGCAT GTTGGGGCGA GGACTCTGAA GGCGACACTG GCACCATCAA ACGGAGAGGA
    GGGAGGGATG TGACCACAGA TCCAGAGACC AGCAGCATCA CCTCCACCGG GTCGGAAGAG
    TCCAGACAGC ACAGCCGACG GCCCTCACCC ATCACCGCAG GAAACACCAA GGCTTCCATC
    ACACGGAAGG ACGGCCGCTA TCGGGACCCT CCACCAACGC CGCCGGGTTA CACCGCGCTG
    ACCATCACAG ATGTGACCGA GGGAACGCCA CATTCGGGCA GGAAACCACC AGACTACACC
    ACCGCCCTGC AGCGCTCGCG CCTCGTCACA CACTCCCTCG ACTCCCAGCA GCCCCCAGCC
    GCAGGGAAAC CGGACCCCCG GGGCCGAGAG GAGGACGAGG CAGTGGAGGT GGAGGAGGAG
    GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT CAGAGGAGGA GAGGTCCACA CACAGCAGTT
    CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214668.1
    CDS605..5419
    Translation

    Target ORF information:

    RefSeq Version XM_017359179.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGGTT ATGGACGAGC TGGTGATTAC CTGTCCGACT CTGGCCACAG TGAGATCTCC
    TCCCGCTCCA GTCTGGTGAG CACTTCTTCT CTGGACATGG GGCAGGACGA GCGCCGGCAG
    CGTTACGGAG GAACGGCCGG AGAAGGACAC GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT
    ACTGCTGACC CTGACCAGTA CAGCTTGGGG TCCTACTCCT CCATGCAGGA CTGTCGTGGT
    CTGTACGCAG GCGCCACCGT CCTGTCCTCA CCCAGCTCAG AGGAGCTCAC GCAGGACCAG
    GGTGACCGGG TGTCCCTGGA CGCTGCGGAT TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC
    AGCGGCTCTC ATGATAACAT CCAGACCATG CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA
    GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG AGCTGGGCAT CCGCATGCTC CTCTTCTTCT
    TCGGTGGCAT GTTGGGGCGA GGACTCTGAA GGCGACACTG GCACCATCAA ACGGAGAGGA
    GGGAGGGATG TGACCACAGA TCCAGAGACC AGCAGCATCA CCTCCACCGG GTCGGAAGAG
    TCCAGACAGC ACAGCCGACG GCCCTCACCC ATCACCGCAG GAAACACCAA GGCTTCCATC
    ACACGGAAGG ACGGCCGCTA TCGGGACCCT CCACCAACGC CGCCGGGTTA CACCGCGCTG
    ACCATCACAG ATGTGACCGA GGGAACGCCA CATTCGGGCA GGAAACCACC AGACTACACC
    ACCGCCCTGC AGCGCTCGCG CCTCGTCACA CACTCCCTCG ACTCCCAGCA GCCCCCAGCC
    GCAGGGAAAC CGGACCCCCG GGGCCGAGAG GAGGACGAGG CAGTGGAGGT GGAGGAGGAG
    GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT CAGAGGAGGA GAGGTCCACA CACAGCAGTT
    CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55036
    Clone ID Related Accession (Same CDS sequence) XM_017359179.1 , XM_017359179.2
    Accession Version XM_017359179.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGGTT ATGGACGAGC TGGTGATTAC CTGTCCGACT CTGGCCACAG TGAGATCTCC
    TCCCGCTCCA GTCTGGTGAG CACTTCTTCT CTGGACATGG GGCAGGACGA GCGCCGGCAG
    CGTTACGGAG GAACGGCCGG AGAAGGACAC GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT
    ACTGCTGACC CTGACCAGTA CAGCTTGGGG TCCTACTCCT CCATGCAGGA CTGTCGTGGT
    CTGTACGCAG GCGCCACCGT CCTGTCCTCA CCCAGCTCAG AGGAGCTCAC GCAGGACCAG
    GGTGACCGGG TGTCCCTGGA CGCTGCGGAT TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC
    AGCGGCTCTC ATGATAACAT CCAGACCATG CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA
    GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG AGCTGGGCAT CCGCATGCTC CTCTTCTTCT
    TCGGTGGCAT GTTGGGGCGA GGACTCTGAA GGCGACACTG GCACCATCAA ACGGAGAGGA
    GGGAGGGATG TGACCACAGA TCCAGAGACC AGCAGCATCA CCTCCACCGG GTCGGAAGAG
    TCCAGACAGC ACAGCCGACG GCCCTCACCC ATCACCGCAG GAAACACCAA GGCTTCCATC
    ACACGGAAGG ACGGCCGCTA TCGGGACCCT CCACCAACGC CGCCGGGTTA CACCGCGCTG
    ACCATCACAG ATGTGACCGA GGGAACGCCA CATTCGGGCA GGAAACCACC AGACTACACC
    ACCGCCCTGC AGCGCTCGCG CCTCGTCACA CACTCCCTCG ACTCCCAGCA GCCCCCAGCC
    GCAGGGAAAC CGGACCCCCG GGGCCGAGAG GAGGACGAGG CAGTGGAGGT GGAGGAGGAG
    GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT CAGAGGAGGA GAGGTCCACA CACAGCAGTT
    CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214668.1
    CDS542..5356
    Translation

    Target ORF information:

    RefSeq Version XM_017359179.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359179.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGTCACTCA GCCAGGGCAG TGCCAACGCG GCTGTCCTGG ATGTGACCCA GACGGGTGGG
    CACAAGAAGC GGGTCAGAAG GAGTTCCTTC CTTAATGCCA AGAAGCTGTA CGAGGACGCA
    CAGATGGCAC GCAAAGTCAA ACAATACCTG TCCAACCTCA CCCTGGAGAC CAACGAGGAG
    AGCCTGCAAA CGCTCTCAAT GCAGTGTGAG CCCTCAATCA ACACCTTGCC GAAGAGTTCT
    GGGGACAGAA AGAGACCCGA CACATCACCC GTAGTCTCAC GGGCTGCGAC CCACCAGCGC
    CAGCAGCTTC AAAAAGCCAA CCAGGCGCTT CAAGTGCCTG CCGTAGCCCT CTACCCATCC
    CGCAAAAAAG TGCCAGTCAA AGACTTACCG CCATTTGGCA CAAGCTCACC ACAGTCTCTG
    AAGAAGATCC TGTCCCTTTC AGAGGAGGCA GGAGACAGAC ACCGAAAGCA GGCAGAGGAC
    AGTGTGTCCA ACAACTCCTC ACAGCTCTCG TCTCCTCCAA CATCTCCTCA CAGCTCACCC
    AAAAAAGGTT ATGGACGAGC TGGTGATTAC CTGTCCGACT CTGGCCACAG TGAGATCTCC
    TCCCGCTCCA GTCTGGTGAG CACTTCTTCT CTGGACATGG GGCAGGACGA GCGCCGGCAG
    CGTTACGGAG GAACGGCCGG AGAAGGACAC GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT
    ACTGCTGACC CTGACCAGTA CAGCTTGGGG TCCTACTCCT CCATGCAGGA CTGTCGTGGT
    CTGTACGCAG GCGCCACCGT CCTGTCCTCA CCCAGCTCAG AGGAGCTCAC GCAGGACCAG
    GGTGACCGGG TGTCCCTGGA CGCTGCGGAT TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC
    AGCGGCTCTC ATGATAACAT CCAGACCATG CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA
    GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG AGCTGGGCAT CCGCATGCTC CTCTTCTTCT
    TCGGTGGCAT GTTGGGGCGA GGACTCTGAA GGCGACACTG GCACCATCAA ACGGAGAGGA
    GGGAGGGATG TGACCACAGA TCCAGAGACC AGCAGCATCA CCTCCACCGG GTCGGAAGAG
    TCCAGACAGC ACAGCCGACG GCCCTCACCC ATCACCGCAG GAAACACCAA GGCTTCCATC
    ACACGGAAGG ACGGCCGCTA TCGGGACCCT CCACCAACGC CGCCGGGTTA CACCGCGCTG
    ACCATCACAG ATGTGACCGA GGGAACGCCA CATTCGGGCA GGAAACCACC AGACTACACC
    ACCGCCCTGC AGCGCTCGCG CCTCGTCACA CACTCCCTCG ACTCCCAGCA GCCCCCAGCC
    GCAGGGAAAC CGGACCCCCG GGGCCGAGAG GAGGACGAGG CAGTGGAGGT GGAGGAGGAG
    GGTGAGTGCT TGTCTCCCAA ACTCAGAGCT CAGAGGAGGA GAGGTCCACA CACAGCAGTT
    CCCCCCAGGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55037
    Clone ID Related Accession (Same CDS sequence) XM_017359180.1 , XM_017359180.2
    Accession Version XM_017359180.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGACATG TCAGACCAGG TATTGCGAGT GTTCAAGGCG
    GACCAGCAAA GTCGGTACAT CATGATTGGG AAGGACACCA CGGCAAAAGA AGTGGTGGCT
    CAGGCCATCC GAGAGTTCGC CCTCACCGCT GCTGCCGAAG CCTATTCGTT GTGTGAGGTG
    TCAGTCACTC CCGAAGGAGT CATCAAGCAA AGGAGACTCC CTGAACAACT CTCCAAACTG
    GCTGACAGGA TCCAGCTGAG TGGAAGGTAC TACCTGAAAA GCAACATGGA GACAGAGACA
    CTGTGCTCGG ATGAAGATGC GCAGGAGTTG CTACGAGAGA GCCATATCAG CCTGCTGCAG
    CTCAGCACGG TGGAAGTGGC AACCCAACTC TCCATGCGCG CCTTTGAGCT GTTCTGTGCC
    ATCGAGCCCA CCGAGTACAT CGATGACCTG TTTAAGCTGA AAACTCGCCT CACCGGACCC
    CCCAGCCTCA AGCTTTTTGA GGAGGCCATC AATCGGGAGA CTTTCTGGGT GGCCACAGAG
    GTGGTGCGAG AGGCCAACCA GCTCAAACGC ATGAAGATTG TCAAGCACTT CATTAAGATT
    GCACTGCACT GCCGCGAATG CAAGAACTTC AACTCCATGT TTGCCATCAT CAGTGGTCTG
    AACTTGGCAC CAGTGTCCAG GCTGAGAGGC ACATGGGAGA AGCTGCCCAG CAAATACGAG
    AAGCTGTTTA GCGATCTTCA AGACCTCTTC GATCCCTCCA GGAATATGGC CAAGTATCGC
    AACCTTCTAA ACAGCCAGAA CCTGCAGCCC CCTATCATCC CACTCTTCCC CGTAATCAAG
    AAGGATCTCA CTTTCCTGCA TGAAGGGAAT GACTCCAAAG TGGACGGTTT GGTGAACTTT
    GAGAAGCTGC GGATGATCGC TAAAGAGATC CGACACGTGG GCAGAATGGC AGCCGTAAAC
    ATGGACCCTG CACTGATGTT CAGAACCAGG AAGAAGAAAT GGAGGAGTTT AGGGTCACTC
    AGCCAGGGCA GTGCCAACGC GGCTGTCCTG GATGTGACCC AGACGGGTGG GCACAAGAAG
    CGGGTCAGAA GGAGTTCCTT CCTTAATGCC AAGAAGCTGT ACGAGGACGC ACAGATGGCA
    CGCAAAGTCA AACAATACCT GTCCAACCTC ACCCTGGAGA CCAACGAGGA GAGCCTGCAA
    ACGCTCTCAA TGCAGTGTGA GCCCTCAATC AACACCTTGC CGAAGAGTTC TGGGGACAGA
    AAGAGACCCG ACACATCACC CGTAGTCTCA CGGGCTGCGA CCCACCAGCG CCAGCAGCTT
    CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT GCCGTAGCCC TCTACCCATC CCGCAAAAAA
    GTGCCAGTCA AAGACTTACC GCCATTTGGC ACAAGCTCAC CACAGTCTCT GAAGAAGATC
    CTGTCCCTTT CAGAGGAGGC AGGAGACAGA CACCGAAAGC AGGCAGAGGA CAGTGTGTCC
    AACAACTCCT CACAGCTCTC GTCTCCTCCA ACATCTCCTC ACAGCTCACC CAAAAAAGGT
    TATGGACGAG CTGGTGATTA CCTGTCCGAC TCTGGCCACA GTGAGATCTC CTCCCGCTCC
    AGTCTGGTGA GCACTTCTTC TCTGGACATG GGGCAGGACG AGCGCCGGCA GCGTTACGGA
    GGAACGGCCG GAGAAGGACA CGGCAGTGGC CACCGGCTCG AGCGCAGAGC TACTGCTGAC
    CCTGACCAGT ACAGCTTGGG GTCCTACTCC TCCATGCAGG ACTGTCGTGG TCTGTACGCA
    GGCGCCACCG TCCTGTCCTC ACCCAGCTCA GAGGAGCTCA CGCAGGACCA GGGTGACCGG
    GTGTCCCTGG ACGCTGCGGA TTCCGGTCGT GGCAGCTGGA CTTCGTGCTC CAGCGGCTCT
    CATGATAACA TCCAGACCAT GCAGCAGGGC CGCAGCTGGG AGGCGCTGGC AGAGGGCGCA
    GGCCTGTGGG GCAGCAGAGG GAGCTGGGCA TCCGCATGCT CCTCTTCTTC TTCGGTGGCA
    TGTTGGGGCG AGGACTCTGA AGGCGACACT GGCACCATCA AACGGAGAGG AGGGAGGGAT
    GTGACCACAG ATCCAGAGAC CAGCAGCATC ACCTCCACCG GGTCGGAAGA GTCCAGACAG
    CACAGCCGAC GGCCCTCACC CATCACCGCA GGAAACACCA AGGCTTCCAT CACACGGAAG
    GACGGCCGCT ATCGGGACCC TCCACCAACG CCGCCGGGTT ACACCGCGCT GACCATCACA
    GATGTGACCG AGGGAACGCC ACATTCGGGC AGGAAACCAC CAGACTACAC CACCGCCCTG
    CAGCGCTCGC GCCTCGTCAC ACACTCCCTC GACTCCCAGC AGCCCCCAGC CGCAGGGAAA
    CCGGACCCCC GGGGCCGAGA GGAGGACGAG GCAGTGGAGG TGGAGGAGGA GGGTGAGTGC
    TTGTCTCCCA AACTCAGAGC TCAGAGGAGG AGAGGTCCAC ACACAGCAGT TCCCCCCAGG
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214669.1
    CDS605..5410
    Translation

    Target ORF information:

    RefSeq Version XM_017359180.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGACATG TCAGACCAGG TATTGCGAGT GTTCAAGGCG
    GACCAGCAAA GTCGGTACAT CATGATTGGG AAGGACACCA CGGCAAAAGA AGTGGTGGCT
    CAGGCCATCC GAGAGTTCGC CCTCACCGCT GCTGCCGAAG CCTATTCGTT GTGTGAGGTG
    TCAGTCACTC CCGAAGGAGT CATCAAGCAA AGGAGACTCC CTGAACAACT CTCCAAACTG
    GCTGACAGGA TCCAGCTGAG TGGAAGGTAC TACCTGAAAA GCAACATGGA GACAGAGACA
    CTGTGCTCGG ATGAAGATGC GCAGGAGTTG CTACGAGAGA GCCATATCAG CCTGCTGCAG
    CTCAGCACGG TGGAAGTGGC AACCCAACTC TCCATGCGCG CCTTTGAGCT GTTCTGTGCC
    ATCGAGCCCA CCGAGTACAT CGATGACCTG TTTAAGCTGA AAACTCGCCT CACCGGACCC
    CCCAGCCTCA AGCTTTTTGA GGAGGCCATC AATCGGGAGA CTTTCTGGGT GGCCACAGAG
    GTGGTGCGAG AGGCCAACCA GCTCAAACGC ATGAAGATTG TCAAGCACTT CATTAAGATT
    GCACTGCACT GCCGCGAATG CAAGAACTTC AACTCCATGT TTGCCATCAT CAGTGGTCTG
    AACTTGGCAC CAGTGTCCAG GCTGAGAGGC ACATGGGAGA AGCTGCCCAG CAAATACGAG
    AAGCTGTTTA GCGATCTTCA AGACCTCTTC GATCCCTCCA GGAATATGGC CAAGTATCGC
    AACCTTCTAA ACAGCCAGAA CCTGCAGCCC CCTATCATCC CACTCTTCCC CGTAATCAAG
    AAGGATCTCA CTTTCCTGCA TGAAGGGAAT GACTCCAAAG TGGACGGTTT GGTGAACTTT
    GAGAAGCTGC GGATGATCGC TAAAGAGATC CGACACGTGG GCAGAATGGC AGCCGTAAAC
    ATGGACCCTG CACTGATGTT CAGAACCAGG AAGAAGAAAT GGAGGAGTTT AGGGTCACTC
    AGCCAGGGCA GTGCCAACGC GGCTGTCCTG GATGTGACCC AGACGGGTGG GCACAAGAAG
    CGGGTCAGAA GGAGTTCCTT CCTTAATGCC AAGAAGCTGT ACGAGGACGC ACAGATGGCA
    CGCAAAGTCA AACAATACCT GTCCAACCTC ACCCTGGAGA CCAACGAGGA GAGCCTGCAA
    ACGCTCTCAA TGCAGTGTGA GCCCTCAATC AACACCTTGC CGAAGAGTTC TGGGGACAGA
    AAGAGACCCG ACACATCACC CGTAGTCTCA CGGGCTGCGA CCCACCAGCG CCAGCAGCTT
    CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT GCCGTAGCCC TCTACCCATC CCGCAAAAAA
    GTGCCAGTCA AAGACTTACC GCCATTTGGC ACAAGCTCAC CACAGTCTCT GAAGAAGATC
    CTGTCCCTTT CAGAGGAGGC AGGAGACAGA CACCGAAAGC AGGCAGAGGA CAGTGTGTCC
    AACAACTCCT CACAGCTCTC GTCTCCTCCA ACATCTCCTC ACAGCTCACC CAAAAAAGGT
    TATGGACGAG CTGGTGATTA CCTGTCCGAC TCTGGCCACA GTGAGATCTC CTCCCGCTCC
    AGTCTGGTGA GCACTTCTTC TCTGGACATG GGGCAGGACG AGCGCCGGCA GCGTTACGGA
    GGAACGGCCG GAGAAGGACA CGGCAGTGGC CACCGGCTCG AGCGCAGAGC TACTGCTGAC
    CCTGACCAGT ACAGCTTGGG GTCCTACTCC TCCATGCAGG ACTGTCGTGG TCTGTACGCA
    GGCGCCACCG TCCTGTCCTC ACCCAGCTCA GAGGAGCTCA CGCAGGACCA GGGTGACCGG
    GTGTCCCTGG ACGCTGCGGA TTCCGGTCGT GGCAGCTGGA CTTCGTGCTC CAGCGGCTCT
    CATGATAACA TCCAGACCAT GCAGCAGGGC CGCAGCTGGG AGGCGCTGGC AGAGGGCGCA
    GGCCTGTGGG GCAGCAGAGG GAGCTGGGCA TCCGCATGCT CCTCTTCTTC TTCGGTGGCA
    TGTTGGGGCG AGGACTCTGA AGGCGACACT GGCACCATCA AACGGAGAGG AGGGAGGGAT
    GTGACCACAG ATCCAGAGAC CAGCAGCATC ACCTCCACCG GGTCGGAAGA GTCCAGACAG
    CACAGCCGAC GGCCCTCACC CATCACCGCA GGAAACACCA AGGCTTCCAT CACACGGAAG
    GACGGCCGCT ATCGGGACCC TCCACCAACG CCGCCGGGTT ACACCGCGCT GACCATCACA
    GATGTGACCG AGGGAACGCC ACATTCGGGC AGGAAACCAC CAGACTACAC CACCGCCCTG
    CAGCGCTCGC GCCTCGTCAC ACACTCCCTC GACTCCCAGC AGCCCCCAGC CGCAGGGAAA
    CCGGACCCCC GGGGCCGAGA GGAGGACGAG GCAGTGGAGG TGGAGGAGGA GGGTGAGTGC
    TTGTCTCCCA AACTCAGAGC TCAGAGGAGG AGAGGTCCAC ACACAGCAGT TCCCCCCAGG
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55037
    Clone ID Related Accession (Same CDS sequence) XM_017359180.1 , XM_017359180.2
    Accession Version XM_017359180.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGACATG TCAGACCAGG TATTGCGAGT GTTCAAGGCG
    GACCAGCAAA GTCGGTACAT CATGATTGGG AAGGACACCA CGGCAAAAGA AGTGGTGGCT
    CAGGCCATCC GAGAGTTCGC CCTCACCGCT GCTGCCGAAG CCTATTCGTT GTGTGAGGTG
    TCAGTCACTC CCGAAGGAGT CATCAAGCAA AGGAGACTCC CTGAACAACT CTCCAAACTG
    GCTGACAGGA TCCAGCTGAG TGGAAGGTAC TACCTGAAAA GCAACATGGA GACAGAGACA
    CTGTGCTCGG ATGAAGATGC GCAGGAGTTG CTACGAGAGA GCCATATCAG CCTGCTGCAG
    CTCAGCACGG TGGAAGTGGC AACCCAACTC TCCATGCGCG CCTTTGAGCT GTTCTGTGCC
    ATCGAGCCCA CCGAGTACAT CGATGACCTG TTTAAGCTGA AAACTCGCCT CACCGGACCC
    CCCAGCCTCA AGCTTTTTGA GGAGGCCATC AATCGGGAGA CTTTCTGGGT GGCCACAGAG
    GTGGTGCGAG AGGCCAACCA GCTCAAACGC ATGAAGATTG TCAAGCACTT CATTAAGATT
    GCACTGCACT GCCGCGAATG CAAGAACTTC AACTCCATGT TTGCCATCAT CAGTGGTCTG
    AACTTGGCAC CAGTGTCCAG GCTGAGAGGC ACATGGGAGA AGCTGCCCAG CAAATACGAG
    AAGCTGTTTA GCGATCTTCA AGACCTCTTC GATCCCTCCA GGAATATGGC CAAGTATCGC
    AACCTTCTAA ACAGCCAGAA CCTGCAGCCC CCTATCATCC CACTCTTCCC CGTAATCAAG
    AAGGATCTCA CTTTCCTGCA TGAAGGGAAT GACTCCAAAG TGGACGGTTT GGTGAACTTT
    GAGAAGCTGC GGATGATCGC TAAAGAGATC CGACACGTGG GCAGAATGGC AGCCGTAAAC
    ATGGACCCTG CACTGATGTT CAGAACCAGG AAGAAGAAAT GGAGGAGTTT AGGGTCACTC
    AGCCAGGGCA GTGCCAACGC GGCTGTCCTG GATGTGACCC AGACGGGTGG GCACAAGAAG
    CGGGTCAGAA GGAGTTCCTT CCTTAATGCC AAGAAGCTGT ACGAGGACGC ACAGATGGCA
    CGCAAAGTCA AACAATACCT GTCCAACCTC ACCCTGGAGA CCAACGAGGA GAGCCTGCAA
    ACGCTCTCAA TGCAGTGTGA GCCCTCAATC AACACCTTGC CGAAGAGTTC TGGGGACAGA
    AAGAGACCCG ACACATCACC CGTAGTCTCA CGGGCTGCGA CCCACCAGCG CCAGCAGCTT
    CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT GCCGTAGCCC TCTACCCATC CCGCAAAAAA
    GTGCCAGTCA AAGACTTACC GCCATTTGGC ACAAGCTCAC CACAGTCTCT GAAGAAGATC
    CTGTCCCTTT CAGAGGAGGC AGGAGACAGA CACCGAAAGC AGGCAGAGGA CAGTGTGTCC
    AACAACTCCT CACAGCTCTC GTCTCCTCCA ACATCTCCTC ACAGCTCACC CAAAAAAGGT
    TATGGACGAG CTGGTGATTA CCTGTCCGAC TCTGGCCACA GTGAGATCTC CTCCCGCTCC
    AGTCTGGTGA GCACTTCTTC TCTGGACATG GGGCAGGACG AGCGCCGGCA GCGTTACGGA
    GGAACGGCCG GAGAAGGACA CGGCAGTGGC CACCGGCTCG AGCGCAGAGC TACTGCTGAC
    CCTGACCAGT ACAGCTTGGG GTCCTACTCC TCCATGCAGG ACTGTCGTGG TCTGTACGCA
    GGCGCCACCG TCCTGTCCTC ACCCAGCTCA GAGGAGCTCA CGCAGGACCA GGGTGACCGG
    GTGTCCCTGG ACGCTGCGGA TTCCGGTCGT GGCAGCTGGA CTTCGTGCTC CAGCGGCTCT
    CATGATAACA TCCAGACCAT GCAGCAGGGC CGCAGCTGGG AGGCGCTGGC AGAGGGCGCA
    GGCCTGTGGG GCAGCAGAGG GAGCTGGGCA TCCGCATGCT CCTCTTCTTC TTCGGTGGCA
    TGTTGGGGCG AGGACTCTGA AGGCGACACT GGCACCATCA AACGGAGAGG AGGGAGGGAT
    GTGACCACAG ATCCAGAGAC CAGCAGCATC ACCTCCACCG GGTCGGAAGA GTCCAGACAG
    CACAGCCGAC GGCCCTCACC CATCACCGCA GGAAACACCA AGGCTTCCAT CACACGGAAG
    GACGGCCGCT ATCGGGACCC TCCACCAACG CCGCCGGGTT ACACCGCGCT GACCATCACA
    GATGTGACCG AGGGAACGCC ACATTCGGGC AGGAAACCAC CAGACTACAC CACCGCCCTG
    CAGCGCTCGC GCCTCGTCAC ACACTCCCTC GACTCCCAGC AGCCCCCAGC CGCAGGGAAA
    CCGGACCCCC GGGGCCGAGA GGAGGACGAG GCAGTGGAGG TGGAGGAGGA GGGTGAGTGC
    TTGTCTCCCA AACTCAGAGC TCAGAGGAGG AGAGGTCCAC ACACAGCAGT TCCCCCCAGG
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214669.1
    CDS541..5346
    Translation

    Target ORF information:

    RefSeq Version XM_017359180.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359180.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGCTGCC GGCAGACTTC
    AGCAGGTTAC ACCTGGCAGA CGGGATTCAC CCTCAGGTGA CACACGTGTC CTCCAGTCAT
    TCAGGATGTA GCATCACCAG TGACTCTGGA AGCAGCAGCC TGTCGGACAT TTACCAGGCC
    ACCGAGAGTG AGCCGGGCGA CATGGACCTC AGCGGTCTGC CTGAGACGGC CGTTGACTCC
    GAGGAGGACG ATGATGAGGA AGACATCGAG CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC
    ATCGTCCGAG ACTGCCTGGA GAAAGACCCC ATGGACCGAA CAGATGATGA CATAGAGCAA
    CTGTTGGAGT TCATGCACCA GCTACCAGCC TTTGCCAACA TGACCATGTC TGTCAGGAGG
    GAGCTGTGTG CTGTCATGGT GTTCGCTGTG GTGGAACGGG CCGGCACCAT CGTCCTTAAT
    GATGGCGAAG AGCTGGACTC GTGGTCTGTG ATTCTGAATG GATCGGTGGA AGTGACGCAT
    CCAGACAGCC GCAATGAAAT CCTCTGCATG GGCAACAGCT TCGGCGTTTC TCCCACCATG
    GAGAAAGAGT ACATGAAGGG GGTGATGAAG ACCAAGGTTG ATGACTGCCA GTTTGTGTGC
    ATCGCTCAGC AGGATTACTG CTGCATCCTC AACCAGGTGG AGAAGAACAT GCAGAAGGTG
    GAGGAGGAAG GAGAGATCGT GATGGTGAAG GAGCACAGAG AGCTGGACCG CACAGGCACC
    AGGAAAGGGC ACATCGTCAT CAAGGGCACA TCAGAGCGTC TCACCATGCA CCTTGTAGAA
    GAGCACTCAG TGGTCGACCC GACTTACATC GAGGACTTCC TGCTCACCTA CCGCACCTTC
    CTGCCGAGCC CCATGGTGGT GGGCAAGAGA CTGTTGGAGT GGTTTCATGA CCCCAGCCTC
    AGGGACAAGG TTACACGGGT AGTCTTGCTG TGGGTGAACA ATCACTTTAA TGACTTTGAG
    GGCGACCCTG CAATGACACA TTTTCTGGAG GAGTTCGAGT ACAATCTGGA GAGAGAGAAA
    ATGTGTGGCC ACCTAAGACT GTTAAACATA GCATGTGCTG CCAAAGCTAA GCTGCGTGTG
    GTGACCCTGA CCAAACCCTC GAGGGAAGCC CCGCTGGCCT TCACCCTGCT GGGCGGCTCC
    GAGAAGGGCT TTCGCATCTT CATCGACAGC GTGGAACCCG GCAGTAAAGC TGCAGAGGCC
    GGCCTTAAAA GAGGAGATCA GATACTGGAG GTAAATGGGC AAAACTTTGA AAATGTGCCG
    CTATCCAAAG CCAACGAGAT TCTCAAAAAC AACACGCACC TGTCCATTAC TGTGAAAACC
    AATCTCTTGG TGTTTAAGGA GCTGCTGGCC AGGCCTGACC AGGATCAGGA GCAAGAACAA
    GAAGAGGAGC CCGACCGGAA AAACGGGGCT CCGCACATCC CTAAAATCGG GGACATTAAG
    AAAGCCAGCC GCTACTCCAT CCCTGACCTG GCTGTGGACG TGGAGCAGGT CATGGATCTG
    GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC AGCGCAGGCG GAAGAAACAA GCTGAAGAAG
    ATCTTCGACA AGACACTGAC CAGTATTCTT CCACCCAAAC CTTACAATGA CGTTGGGGTG
    GGACAGTCCC AGGATGACAG TATTGTGGGC CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT
    CTCCCTGTCA GTGGAAACCT TTCATCAAGC AACCCTGACC TGCTGCAGTC ACACCATCGC
    ATACTCGACT TCAACAACCA GCCTGACATG TCAGACCAGG TATTGCGAGT GTTCAAGGCG
    GACCAGCAAA GTCGGTACAT CATGATTGGG AAGGACACCA CGGCAAAAGA AGTGGTGGCT
    CAGGCCATCC GAGAGTTCGC CCTCACCGCT GCTGCCGAAG CCTATTCGTT GTGTGAGGTG
    TCAGTCACTC CCGAAGGAGT CATCAAGCAA AGGAGACTCC CTGAACAACT CTCCAAACTG
    GCTGACAGGA TCCAGCTGAG TGGAAGGTAC TACCTGAAAA GCAACATGGA GACAGAGACA
    CTGTGCTCGG ATGAAGATGC GCAGGAGTTG CTACGAGAGA GCCATATCAG CCTGCTGCAG
    CTCAGCACGG TGGAAGTGGC AACCCAACTC TCCATGCGCG CCTTTGAGCT GTTCTGTGCC
    ATCGAGCCCA CCGAGTACAT CGATGACCTG TTTAAGCTGA AAACTCGCCT CACCGGACCC
    CCCAGCCTCA AGCTTTTTGA GGAGGCCATC AATCGGGAGA CTTTCTGGGT GGCCACAGAG
    GTGGTGCGAG AGGCCAACCA GCTCAAACGC ATGAAGATTG TCAAGCACTT CATTAAGATT
    GCACTGCACT GCCGCGAATG CAAGAACTTC AACTCCATGT TTGCCATCAT CAGTGGTCTG
    AACTTGGCAC CAGTGTCCAG GCTGAGAGGC ACATGGGAGA AGCTGCCCAG CAAATACGAG
    AAGCTGTTTA GCGATCTTCA AGACCTCTTC GATCCCTCCA GGAATATGGC CAAGTATCGC
    AACCTTCTAA ACAGCCAGAA CCTGCAGCCC CCTATCATCC CACTCTTCCC CGTAATCAAG
    AAGGATCTCA CTTTCCTGCA TGAAGGGAAT GACTCCAAAG TGGACGGTTT GGTGAACTTT
    GAGAAGCTGC GGATGATCGC TAAAGAGATC CGACACGTGG GCAGAATGGC AGCCGTAAAC
    ATGGACCCTG CACTGATGTT CAGAACCAGG AAGAAGAAAT GGAGGAGTTT AGGGTCACTC
    AGCCAGGGCA GTGCCAACGC GGCTGTCCTG GATGTGACCC AGACGGGTGG GCACAAGAAG
    CGGGTCAGAA GGAGTTCCTT CCTTAATGCC AAGAAGCTGT ACGAGGACGC ACAGATGGCA
    CGCAAAGTCA AACAATACCT GTCCAACCTC ACCCTGGAGA CCAACGAGGA GAGCCTGCAA
    ACGCTCTCAA TGCAGTGTGA GCCCTCAATC AACACCTTGC CGAAGAGTTC TGGGGACAGA
    AAGAGACCCG ACACATCACC CGTAGTCTCA CGGGCTGCGA CCCACCAGCG CCAGCAGCTT
    CAAAAAGCCA ACCAGGCGCT TCAAGTGCCT GCCGTAGCCC TCTACCCATC CCGCAAAAAA
    GTGCCAGTCA AAGACTTACC GCCATTTGGC ACAAGCTCAC CACAGTCTCT GAAGAAGATC
    CTGTCCCTTT CAGAGGAGGC AGGAGACAGA CACCGAAAGC AGGCAGAGGA CAGTGTGTCC
    AACAACTCCT CACAGCTCTC GTCTCCTCCA ACATCTCCTC ACAGCTCACC CAAAAAAGGT
    TATGGACGAG CTGGTGATTA CCTGTCCGAC TCTGGCCACA GTGAGATCTC CTCCCGCTCC
    AGTCTGGTGA GCACTTCTTC TCTGGACATG GGGCAGGACG AGCGCCGGCA GCGTTACGGA
    GGAACGGCCG GAGAAGGACA CGGCAGTGGC CACCGGCTCG AGCGCAGAGC TACTGCTGAC
    CCTGACCAGT ACAGCTTGGG GTCCTACTCC TCCATGCAGG ACTGTCGTGG TCTGTACGCA
    GGCGCCACCG TCCTGTCCTC ACCCAGCTCA GAGGAGCTCA CGCAGGACCA GGGTGACCGG
    GTGTCCCTGG ACGCTGCGGA TTCCGGTCGT GGCAGCTGGA CTTCGTGCTC CAGCGGCTCT
    CATGATAACA TCCAGACCAT GCAGCAGGGC CGCAGCTGGG AGGCGCTGGC AGAGGGCGCA
    GGCCTGTGGG GCAGCAGAGG GAGCTGGGCA TCCGCATGCT CCTCTTCTTC TTCGGTGGCA
    TGTTGGGGCG AGGACTCTGA AGGCGACACT GGCACCATCA AACGGAGAGG AGGGAGGGAT
    GTGACCACAG ATCCAGAGAC CAGCAGCATC ACCTCCACCG GGTCGGAAGA GTCCAGACAG
    CACAGCCGAC GGCCCTCACC CATCACCGCA GGAAACACCA AGGCTTCCAT CACACGGAAG
    GACGGCCGCT ATCGGGACCC TCCACCAACG CCGCCGGGTT ACACCGCGCT GACCATCACA
    GATGTGACCG AGGGAACGCC ACATTCGGGC AGGAAACCAC CAGACTACAC CACCGCCCTG
    CAGCGCTCGC GCCTCGTCAC ACACTCCCTC GACTCCCAGC AGCCCCCAGC CGCAGGGAAA
    CCGGACCCCC GGGGCCGAGA GGAGGACGAG GCAGTGGAGG TGGAGGAGGA GGGTGAGTGC
    TTGTCTCCCA AACTCAGAGC TCAGAGGAGG AGAGGTCCAC ACACAGCAGT TCCCCCCAGG
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55038
    Clone ID Related Accession (Same CDS sequence) XM_017359181.1 , XM_017359181.2
    Accession Version XM_017359181.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGTGG TGGACTATAT GGATGAGAAT GAGGAGTATT TCCAGCGGCA GGCATCGCAC 
    CGACAGTCAC GCCGACGCTT TCGCAAGATC AACCAGAAGG GCGAGCGGCA GACCATCATC
    GACACCGTAG ACCCCTATCC TGCTGGCAAG CCCCCCGTGG CGCGGGGATA CCATACGGAA
    TGTGTTAAAG CTCAGCTGCC GGCAGACTTC AGCAGGTTAC ACCTGGCAGA CGGGATTCAC
    CCTCAGGTGA CACACGTGTC CTCCAGTCAT TCAGGATGTA GCATCACCAG TGACTCTGGA
    AGCAGCAGCC TGTCGGACAT TTACCAGGCC ACCGAGAGTG AGCCGGGCGA CATGGACCTC
    AGCGGTCTGC CTGAGACGGC CGTTGACTCC GAGGAGGACG ATGATGAGGA AGACATCGAG
    CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC ATCGTCCGAG ACTGCCTGGA GAAAGACCCC
    ATGGACCGAA CAGATGATGA CATAGAGCAA CTGTTGGAGT TCATGCACCA GCTACCAGCC
    TTTGCCAACA TGACCATGTC TGTCAGGAGG GAGCTGTGTG CTGTCATGGT GTTCGCTGTG
    GTGGAACGGG CCGGCACCAT CGTCCTTAAT GATGGCGAAG AGCTGGACTC GTGGTCTGTG
    ATTCTGAATG GATCGGTGGA AGTGACGCAT CCAGACAGCC GCAATGAAAT CCTCTGCATG
    GGCAACAGCT TCGGCGTTTC TCCCACCATG GAGAAAGAGT ACATGAAGGG GGTGATGAAG
    ACCAAGGTTG ATGACTGCCA GTTTGTGTGC ATCGCTCAGC AGGATTACTG CTGCATCCTC
    AACCAGGTGG AGAAGAACAT GCAGAAGGTG GAGGAGGAAG GAGAGATCGT GATGGTGAAG
    GAGCACAGAG AGCTGGACCG CACAGGCACC AGGAAAGGGC ACATCGTCAT CAAGGGCACA
    TCAGAGCGTC TCACCATGCA CCTTGTAGAA GAGCACTCAG TGGTCGACCC GACTTACATC
    GAGGACTTCC TGCTCACCTA CCGCACCTTC CTGCCGAGCC CCATGGTGGT GGGCAAGAGA
    CTGTTGGAGT GGTTTCATGA CCCCAGCCTC AGGGACAAGG TTACACGGGT AGTCTTGCTG
    TGGGTGAACA ATCACTTTAA TGACTTTGAG GGCGACCCTG CAATGACACA TTTTCTGGAG
    GAGTTCGAGT ACAATCTGGA GAGAGAGAAA ATGTGTGGCC ACCTAAGACT GTTAAACATA
    GCATGTGCTG CCAAAGCTAA GCTGCGTGTG GTGACCCTGA CCAAACCCTC GAGGGAAGCC
    CCGCTGGCCT TCACCCTGCT GGGCGGCTCC GAGAAGGGCT TTCGCATCTT CATCGACAGC
    GTGGAACCCG GCAGTAAAGC TGCAGAGGCC GGCCTTAAAA GAGGAGATCA GATACTGGAG
    GTAAATGGGC AAAACTTTGA AAATGTGCCG CTATCCAAAG CCAACGAGAT TCTCAAAAAC
    AACACGCACC TGTCCATTAC TGTGAAAACC AATCTCTTGG TGTTTAAGGA GCTGCTGGCC
    AGGCCTGACC AGGATCAGGA GCAAGAACAA GAAGAGGAGC CCGACCGGAA AAACGGGGCT
    CCGCACATCC CTAAAATCGG GGACATTAAG AAAGCCAGCC GCTACTCCAT CCCTGACCTG
    GCTGTGGACG TGGAGCAGGT CATGGATCTG GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC
    AGCGCAGGCG GAAGAAACAA GCTGAAGAAG ATCTTCGACA AGACACTGAC CAGTATTCTT
    CCACCCAAAC CTTACAATGA CGTTGGGGTG GGACAGTCCC AGGATGACAG TATTGTGGGC
    CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT CTCCCTGTCA GTGGAAACCT TTCATCAAGC
    AACCCTGACC TGCTGCAGTC ACACCATCGC ATACTCGACT TCAACAACCA GCCTGAAGCG
    GTCCCTATAA ACATGTCAGA CCAGGTATTG CGAGTGTTCA AGGCGGACCA GCAAAGTCGG
    TACATCATGA TTGGGAAGGA CACCACGGCA AAAGAAGTGG TGGCTCAGGC CATCCGAGAG
    TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT TCGTTGTGTG AGGTGTCAGT CACTCCCGAA
    GGAGTCATCA AGCAAAGGAG ACTCCCTGAA CAACTCTCCA AACTGGCTGA CAGGATCCAG
    CTGAGTGGAA GGTACTACCT GAAAAGCAAC ATGGAGACAG AGACACTGTG CTCGGATGAA
    GATGCGCAGG AGTTGCTACG AGAGAGCCAT ATCAGCCTGC TGCAGCTCAG CACGGTGGAA
    GTGGCAACCC AACTCTCCAT GCGCGCCTTT GAGCTGTTCT GTGCCATCGA GCCCACCGAG
    TACATCGATG ACCTGTTTAA GCTGAAAACT CGCCTCACCG GACCCCCCAG CCTCAAGCTT
    TTTGAGGAGG CCATCAATCG GGAGACTTTC TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC
    AACCAGCTCA AACGCATGAA GATTGTCAAG CACTTCATTA AGATTGCACT GCACTGCCGC
    GAATGCAAGA ACTTCAACTC CATGTTTGCC ATCATCAGTG GTCTGAACTT GGCACCAGTG
    TCCAGGCTGA GAGGCACATG GGAGAAGCTG CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT
    CTTCAAGACC TCTTCGATCC CTCCAGGAAT ATGGCCAAGT ATCGCAACCT TCTAAACAGC
    CAGAACCTGC AGCCCCCTAT CATCCCACTC TTCCCCGTAA TCAAGAAGGA TCTCACTTTC
    CTGCATGAAG GGAATGACTC CAAAGTGGAC GGTTTGGTGA ACTTTGAGAA GCTGCGGATG
    ATCGCTAAAG AGATCCGACA CGTGGGCAGA ATGGCAGCCG TAAACATGGA CCCTGCACTG
    ATGTTCAGAA CCAGGAAGAA GAAATGGAGG AGTTTAGGGT CACTCAGCCA GGGCAGTGCC
    AACGCGGCTG TCCTGGATGT GACCCAGACG GGTGGGCACA AGAAGCGGGT CAGAAGGAGT
    TCCTTCCTTA ATGCCAAGAA GCTGTACGAG GACGCACAGA TGGCACGCAA AGTCAAACAA
    TACCTGTCCA ACCTCACCCT GGAGACCAAC GAGGAGAGCC TGCAAACGCT CTCAATGCAG
    TGTGAGCCCT CAATCAACAC CTTGCCGAAG AGTTCTGGGG ACAGAAAGAG ACCCGACACA
    TCACCCGTAG TCTCACGGGC TGCGACCCAC CAGCGCCAGC AGCTTCAAAA AGCCAACCAG
    GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC CCATCCCGCA AAAAAGTGCC AGTCAAAGAC
    TTACCGCCAT TTGGCACAAG CTCACCACAG TCTCTGAAGA AGATCCTGTC CCTTTCAGAG
    GAGGCAGGAG ACAGACACCG AAAGCAGGCA GAGGACAGTG TGTCCAACAA CTCCTCACAG
    CTCTCGTCTC CTCCAACATC TCCTCACAGC TCACCCAAAA AAGCCATCGC ATCAATAAGA
    AGTCATGGTA TGAAGGGCTT TTCCTCCTTG TGTAGTAAAT CACCAACAGG TTATGGACGA
    GCTGGTGATT ACCTGTCCGA CTCTGGCCAC AGTGAGATCT CCTCCCGCTC CAGTCTGGTG
    AGCACTTCTT CTCTGGACAT GGGGCAGGAC GAGCGCCGGC AGCGTTACGG AGGAACGGCC
    GGAGAAGGAC ACGGCAGTGG CCACCGGCTC GAGCGCAGAG CTACTGCTGA CCCTGACCAG
    TACAGCTTGG GGTCCTACTC CTCCATGCAG GACTGTCGTG GTCTGTACGC AGGCGCCACC
    GTCCTGTCCT CACCCAGCTC AGAGGAGCTC ACGCAGGACC AGGGTGACCG GGTGTCCCTG
    GACGCTGCGG ATTCCGGTCG TGGCAGCTGG ACTTCGTGCT CCAGCGGCTC TCATGATAAC
    ATCCAGACCA TGCAGCAGGG CCGCAGCTGG GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG
    GGCAGCAGAG GGAGCTGGGC ATCCGCATGC TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC
    GAGGACTCTG AAGGCGACAC TGGCACCATC AAACGGAGAG GAGGGAGGGA TGTGACCACA
    GATCCAGAGA CCAGCAGCAT CACCTCCACC GGGTCGGAAG AGTCCAGACA GCACAGCCGA
    CGGCCCTCAC CCATCACCGC AGGAAACACC AAGGCTTCCA TCACACGGAA GGACGGCCGC
    TATCGGGACC CTCCACCAAC GCCGCCGGGT TACACCGCGC TGACCATCAC AGATGTGACC
    GAGGGAACGC CACATTCGGG CAGGAAACCA CCAGACTACA CCACCGCCCT GCAGCGCTCG
    CGCCTCGTCA CACACTCCCT CGACTCCCAG CAGCCCCCAG CCGCAGGGAA ACCGGACCCC
    CGGGGCCGAG AGGAGGACGA GGCAGTGGAG GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC
    AAACTCAGAG CTCAGAGGAG GAGAGGTCCA CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214670.1
    CDS301..4857
    Translation

    Target ORF information:

    RefSeq Version XM_017359181.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGTGG TGGACTATAT GGATGAGAAT GAGGAGTATT TCCAGCGGCA GGCATCGCAC 
    CGACAGTCAC GCCGACGCTT TCGCAAGATC AACCAGAAGG GCGAGCGGCA GACCATCATC
    GACACCGTAG ACCCCTATCC TGCTGGCAAG CCCCCCGTGG CGCGGGGATA CCATACGGAA
    TGTGTTAAAG CTCAGCTGCC GGCAGACTTC AGCAGGTTAC ACCTGGCAGA CGGGATTCAC
    CCTCAGGTGA CACACGTGTC CTCCAGTCAT TCAGGATGTA GCATCACCAG TGACTCTGGA
    AGCAGCAGCC TGTCGGACAT TTACCAGGCC ACCGAGAGTG AGCCGGGCGA CATGGACCTC
    AGCGGTCTGC CTGAGACGGC CGTTGACTCC GAGGAGGACG ATGATGAGGA AGACATCGAG
    CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC ATCGTCCGAG ACTGCCTGGA GAAAGACCCC
    ATGGACCGAA CAGATGATGA CATAGAGCAA CTGTTGGAGT TCATGCACCA GCTACCAGCC
    TTTGCCAACA TGACCATGTC TGTCAGGAGG GAGCTGTGTG CTGTCATGGT GTTCGCTGTG
    GTGGAACGGG CCGGCACCAT CGTCCTTAAT GATGGCGAAG AGCTGGACTC GTGGTCTGTG
    ATTCTGAATG GATCGGTGGA AGTGACGCAT CCAGACAGCC GCAATGAAAT CCTCTGCATG
    GGCAACAGCT TCGGCGTTTC TCCCACCATG GAGAAAGAGT ACATGAAGGG GGTGATGAAG
    ACCAAGGTTG ATGACTGCCA GTTTGTGTGC ATCGCTCAGC AGGATTACTG CTGCATCCTC
    AACCAGGTGG AGAAGAACAT GCAGAAGGTG GAGGAGGAAG GAGAGATCGT GATGGTGAAG
    GAGCACAGAG AGCTGGACCG CACAGGCACC AGGAAAGGGC ACATCGTCAT CAAGGGCACA
    TCAGAGCGTC TCACCATGCA CCTTGTAGAA GAGCACTCAG TGGTCGACCC GACTTACATC
    GAGGACTTCC TGCTCACCTA CCGCACCTTC CTGCCGAGCC CCATGGTGGT GGGCAAGAGA
    CTGTTGGAGT GGTTTCATGA CCCCAGCCTC AGGGACAAGG TTACACGGGT AGTCTTGCTG
    TGGGTGAACA ATCACTTTAA TGACTTTGAG GGCGACCCTG CAATGACACA TTTTCTGGAG
    GAGTTCGAGT ACAATCTGGA GAGAGAGAAA ATGTGTGGCC ACCTAAGACT GTTAAACATA
    GCATGTGCTG CCAAAGCTAA GCTGCGTGTG GTGACCCTGA CCAAACCCTC GAGGGAAGCC
    CCGCTGGCCT TCACCCTGCT GGGCGGCTCC GAGAAGGGCT TTCGCATCTT CATCGACAGC
    GTGGAACCCG GCAGTAAAGC TGCAGAGGCC GGCCTTAAAA GAGGAGATCA GATACTGGAG
    GTAAATGGGC AAAACTTTGA AAATGTGCCG CTATCCAAAG CCAACGAGAT TCTCAAAAAC
    AACACGCACC TGTCCATTAC TGTGAAAACC AATCTCTTGG TGTTTAAGGA GCTGCTGGCC
    AGGCCTGACC AGGATCAGGA GCAAGAACAA GAAGAGGAGC CCGACCGGAA AAACGGGGCT
    CCGCACATCC CTAAAATCGG GGACATTAAG AAAGCCAGCC GCTACTCCAT CCCTGACCTG
    GCTGTGGACG TGGAGCAGGT CATGGATCTG GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC
    AGCGCAGGCG GAAGAAACAA GCTGAAGAAG ATCTTCGACA AGACACTGAC CAGTATTCTT
    CCACCCAAAC CTTACAATGA CGTTGGGGTG GGACAGTCCC AGGATGACAG TATTGTGGGC
    CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT CTCCCTGTCA GTGGAAACCT TTCATCAAGC
    AACCCTGACC TGCTGCAGTC ACACCATCGC ATACTCGACT TCAACAACCA GCCTGAAGCG
    GTCCCTATAA ACATGTCAGA CCAGGTATTG CGAGTGTTCA AGGCGGACCA GCAAAGTCGG
    TACATCATGA TTGGGAAGGA CACCACGGCA AAAGAAGTGG TGGCTCAGGC CATCCGAGAG
    TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT TCGTTGTGTG AGGTGTCAGT CACTCCCGAA
    GGAGTCATCA AGCAAAGGAG ACTCCCTGAA CAACTCTCCA AACTGGCTGA CAGGATCCAG
    CTGAGTGGAA GGTACTACCT GAAAAGCAAC ATGGAGACAG AGACACTGTG CTCGGATGAA
    GATGCGCAGG AGTTGCTACG AGAGAGCCAT ATCAGCCTGC TGCAGCTCAG CACGGTGGAA
    GTGGCAACCC AACTCTCCAT GCGCGCCTTT GAGCTGTTCT GTGCCATCGA GCCCACCGAG
    TACATCGATG ACCTGTTTAA GCTGAAAACT CGCCTCACCG GACCCCCCAG CCTCAAGCTT
    TTTGAGGAGG CCATCAATCG GGAGACTTTC TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC
    AACCAGCTCA AACGCATGAA GATTGTCAAG CACTTCATTA AGATTGCACT GCACTGCCGC
    GAATGCAAGA ACTTCAACTC CATGTTTGCC ATCATCAGTG GTCTGAACTT GGCACCAGTG
    TCCAGGCTGA GAGGCACATG GGAGAAGCTG CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT
    CTTCAAGACC TCTTCGATCC CTCCAGGAAT ATGGCCAAGT ATCGCAACCT TCTAAACAGC
    CAGAACCTGC AGCCCCCTAT CATCCCACTC TTCCCCGTAA TCAAGAAGGA TCTCACTTTC
    CTGCATGAAG GGAATGACTC CAAAGTGGAC GGTTTGGTGA ACTTTGAGAA GCTGCGGATG
    ATCGCTAAAG AGATCCGACA CGTGGGCAGA ATGGCAGCCG TAAACATGGA CCCTGCACTG
    ATGTTCAGAA CCAGGAAGAA GAAATGGAGG AGTTTAGGGT CACTCAGCCA GGGCAGTGCC
    AACGCGGCTG TCCTGGATGT GACCCAGACG GGTGGGCACA AGAAGCGGGT CAGAAGGAGT
    TCCTTCCTTA ATGCCAAGAA GCTGTACGAG GACGCACAGA TGGCACGCAA AGTCAAACAA
    TACCTGTCCA ACCTCACCCT GGAGACCAAC GAGGAGAGCC TGCAAACGCT CTCAATGCAG
    TGTGAGCCCT CAATCAACAC CTTGCCGAAG AGTTCTGGGG ACAGAAAGAG ACCCGACACA
    TCACCCGTAG TCTCACGGGC TGCGACCCAC CAGCGCCAGC AGCTTCAAAA AGCCAACCAG
    GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC CCATCCCGCA AAAAAGTGCC AGTCAAAGAC
    TTACCGCCAT TTGGCACAAG CTCACCACAG TCTCTGAAGA AGATCCTGTC CCTTTCAGAG
    GAGGCAGGAG ACAGACACCG AAAGCAGGCA GAGGACAGTG TGTCCAACAA CTCCTCACAG
    CTCTCGTCTC CTCCAACATC TCCTCACAGC TCACCCAAAA AAGCCATCGC ATCAATAAGA
    AGTCATGGTA TGAAGGGCTT TTCCTCCTTG TGTAGTAAAT CACCAACAGG TTATGGACGA
    GCTGGTGATT ACCTGTCCGA CTCTGGCCAC AGTGAGATCT CCTCCCGCTC CAGTCTGGTG
    AGCACTTCTT CTCTGGACAT GGGGCAGGAC GAGCGCCGGC AGCGTTACGG AGGAACGGCC
    GGAGAAGGAC ACGGCAGTGG CCACCGGCTC GAGCGCAGAG CTACTGCTGA CCCTGACCAG
    TACAGCTTGG GGTCCTACTC CTCCATGCAG GACTGTCGTG GTCTGTACGC AGGCGCCACC
    GTCCTGTCCT CACCCAGCTC AGAGGAGCTC ACGCAGGACC AGGGTGACCG GGTGTCCCTG
    GACGCTGCGG ATTCCGGTCG TGGCAGCTGG ACTTCGTGCT CCAGCGGCTC TCATGATAAC
    ATCCAGACCA TGCAGCAGGG CCGCAGCTGG GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG
    GGCAGCAGAG GGAGCTGGGC ATCCGCATGC TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC
    GAGGACTCTG AAGGCGACAC TGGCACCATC AAACGGAGAG GAGGGAGGGA TGTGACCACA
    GATCCAGAGA CCAGCAGCAT CACCTCCACC GGGTCGGAAG AGTCCAGACA GCACAGCCGA
    CGGCCCTCAC CCATCACCGC AGGAAACACC AAGGCTTCCA TCACACGGAA GGACGGCCGC
    TATCGGGACC CTCCACCAAC GCCGCCGGGT TACACCGCGC TGACCATCAC AGATGTGACC
    GAGGGAACGC CACATTCGGG CAGGAAACCA CCAGACTACA CCACCGCCCT GCAGCGCTCG
    CGCCTCGTCA CACACTCCCT CGACTCCCAG CAGCCCCCAG CCGCAGGGAA ACCGGACCCC
    CGGGGCCGAG AGGAGGACGA GGCAGTGGAG GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC
    AAACTCAGAG CTCAGAGGAG GAGAGGTCCA CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55038
    Clone ID Related Accession (Same CDS sequence) XM_017359181.1 , XM_017359181.2
    Accession Version XM_017359181.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGTGG TGGACTATAT GGATGAGAAT GAGGAGTATT TCCAGCGGCA GGCATCGCAC 
    CGACAGTCAC GCCGACGCTT TCGCAAGATC AACCAGAAGG GCGAGCGGCA GACCATCATC
    GACACCGTAG ACCCCTATCC TGCTGGCAAG CCCCCCGTGG CGCGGGGATA CCATACGGAA
    TGTGTTAAAG CTCAGCTGCC GGCAGACTTC AGCAGGTTAC ACCTGGCAGA CGGGATTCAC
    CCTCAGGTGA CACACGTGTC CTCCAGTCAT TCAGGATGTA GCATCACCAG TGACTCTGGA
    AGCAGCAGCC TGTCGGACAT TTACCAGGCC ACCGAGAGTG AGCCGGGCGA CATGGACCTC
    AGCGGTCTGC CTGAGACGGC CGTTGACTCC GAGGAGGACG ATGATGAGGA AGACATCGAG
    CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC ATCGTCCGAG ACTGCCTGGA GAAAGACCCC
    ATGGACCGAA CAGATGATGA CATAGAGCAA CTGTTGGAGT TCATGCACCA GCTACCAGCC
    TTTGCCAACA TGACCATGTC TGTCAGGAGG GAGCTGTGTG CTGTCATGGT GTTCGCTGTG
    GTGGAACGGG CCGGCACCAT CGTCCTTAAT GATGGCGAAG AGCTGGACTC GTGGTCTGTG
    ATTCTGAATG GATCGGTGGA AGTGACGCAT CCAGACAGCC GCAATGAAAT CCTCTGCATG
    GGCAACAGCT TCGGCGTTTC TCCCACCATG GAGAAAGAGT ACATGAAGGG GGTGATGAAG
    ACCAAGGTTG ATGACTGCCA GTTTGTGTGC ATCGCTCAGC AGGATTACTG CTGCATCCTC
    AACCAGGTGG AGAAGAACAT GCAGAAGGTG GAGGAGGAAG GAGAGATCGT GATGGTGAAG
    GAGCACAGAG AGCTGGACCG CACAGGCACC AGGAAAGGGC ACATCGTCAT CAAGGGCACA
    TCAGAGCGTC TCACCATGCA CCTTGTAGAA GAGCACTCAG TGGTCGACCC GACTTACATC
    GAGGACTTCC TGCTCACCTA CCGCACCTTC CTGCCGAGCC CCATGGTGGT GGGCAAGAGA
    CTGTTGGAGT GGTTTCATGA CCCCAGCCTC AGGGACAAGG TTACACGGGT AGTCTTGCTG
    TGGGTGAACA ATCACTTTAA TGACTTTGAG GGCGACCCTG CAATGACACA TTTTCTGGAG
    GAGTTCGAGT ACAATCTGGA GAGAGAGAAA ATGTGTGGCC ACCTAAGACT GTTAAACATA
    GCATGTGCTG CCAAAGCTAA GCTGCGTGTG GTGACCCTGA CCAAACCCTC GAGGGAAGCC
    CCGCTGGCCT TCACCCTGCT GGGCGGCTCC GAGAAGGGCT TTCGCATCTT CATCGACAGC
    GTGGAACCCG GCAGTAAAGC TGCAGAGGCC GGCCTTAAAA GAGGAGATCA GATACTGGAG
    GTAAATGGGC AAAACTTTGA AAATGTGCCG CTATCCAAAG CCAACGAGAT TCTCAAAAAC
    AACACGCACC TGTCCATTAC TGTGAAAACC AATCTCTTGG TGTTTAAGGA GCTGCTGGCC
    AGGCCTGACC AGGATCAGGA GCAAGAACAA GAAGAGGAGC CCGACCGGAA AAACGGGGCT
    CCGCACATCC CTAAAATCGG GGACATTAAG AAAGCCAGCC GCTACTCCAT CCCTGACCTG
    GCTGTGGACG TGGAGCAGGT CATGGATCTG GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC
    AGCGCAGGCG GAAGAAACAA GCTGAAGAAG ATCTTCGACA AGACACTGAC CAGTATTCTT
    CCACCCAAAC CTTACAATGA CGTTGGGGTG GGACAGTCCC AGGATGACAG TATTGTGGGC
    CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT CTCCCTGTCA GTGGAAACCT TTCATCAAGC
    AACCCTGACC TGCTGCAGTC ACACCATCGC ATACTCGACT TCAACAACCA GCCTGAAGCG
    GTCCCTATAA ACATGTCAGA CCAGGTATTG CGAGTGTTCA AGGCGGACCA GCAAAGTCGG
    TACATCATGA TTGGGAAGGA CACCACGGCA AAAGAAGTGG TGGCTCAGGC CATCCGAGAG
    TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT TCGTTGTGTG AGGTGTCAGT CACTCCCGAA
    GGAGTCATCA AGCAAAGGAG ACTCCCTGAA CAACTCTCCA AACTGGCTGA CAGGATCCAG
    CTGAGTGGAA GGTACTACCT GAAAAGCAAC ATGGAGACAG AGACACTGTG CTCGGATGAA
    GATGCGCAGG AGTTGCTACG AGAGAGCCAT ATCAGCCTGC TGCAGCTCAG CACGGTGGAA
    GTGGCAACCC AACTCTCCAT GCGCGCCTTT GAGCTGTTCT GTGCCATCGA GCCCACCGAG
    TACATCGATG ACCTGTTTAA GCTGAAAACT CGCCTCACCG GACCCCCCAG CCTCAAGCTT
    TTTGAGGAGG CCATCAATCG GGAGACTTTC TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC
    AACCAGCTCA AACGCATGAA GATTGTCAAG CACTTCATTA AGATTGCACT GCACTGCCGC
    GAATGCAAGA ACTTCAACTC CATGTTTGCC ATCATCAGTG GTCTGAACTT GGCACCAGTG
    TCCAGGCTGA GAGGCACATG GGAGAAGCTG CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT
    CTTCAAGACC TCTTCGATCC CTCCAGGAAT ATGGCCAAGT ATCGCAACCT TCTAAACAGC
    CAGAACCTGC AGCCCCCTAT CATCCCACTC TTCCCCGTAA TCAAGAAGGA TCTCACTTTC
    CTGCATGAAG GGAATGACTC CAAAGTGGAC GGTTTGGTGA ACTTTGAGAA GCTGCGGATG
    ATCGCTAAAG AGATCCGACA CGTGGGCAGA ATGGCAGCCG TAAACATGGA CCCTGCACTG
    ATGTTCAGAA CCAGGAAGAA GAAATGGAGG AGTTTAGGGT CACTCAGCCA GGGCAGTGCC
    AACGCGGCTG TCCTGGATGT GACCCAGACG GGTGGGCACA AGAAGCGGGT CAGAAGGAGT
    TCCTTCCTTA ATGCCAAGAA GCTGTACGAG GACGCACAGA TGGCACGCAA AGTCAAACAA
    TACCTGTCCA ACCTCACCCT GGAGACCAAC GAGGAGAGCC TGCAAACGCT CTCAATGCAG
    TGTGAGCCCT CAATCAACAC CTTGCCGAAG AGTTCTGGGG ACAGAAAGAG ACCCGACACA
    TCACCCGTAG TCTCACGGGC TGCGACCCAC CAGCGCCAGC AGCTTCAAAA AGCCAACCAG
    GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC CCATCCCGCA AAAAAGTGCC AGTCAAAGAC
    TTACCGCCAT TTGGCACAAG CTCACCACAG TCTCTGAAGA AGATCCTGTC CCTTTCAGAG
    GAGGCAGGAG ACAGACACCG AAAGCAGGCA GAGGACAGTG TGTCCAACAA CTCCTCACAG
    CTCTCGTCTC CTCCAACATC TCCTCACAGC TCACCCAAAA AAGCCATCGC ATCAATAAGA
    AGTCATGGTA TGAAGGGCTT TTCCTCCTTG TGTAGTAAAT CACCAACAGG TTATGGACGA
    GCTGGTGATT ACCTGTCCGA CTCTGGCCAC AGTGAGATCT CCTCCCGCTC CAGTCTGGTG
    AGCACTTCTT CTCTGGACAT GGGGCAGGAC GAGCGCCGGC AGCGTTACGG AGGAACGGCC
    GGAGAAGGAC ACGGCAGTGG CCACCGGCTC GAGCGCAGAG CTACTGCTGA CCCTGACCAG
    TACAGCTTGG GGTCCTACTC CTCCATGCAG GACTGTCGTG GTCTGTACGC AGGCGCCACC
    GTCCTGTCCT CACCCAGCTC AGAGGAGCTC ACGCAGGACC AGGGTGACCG GGTGTCCCTG
    GACGCTGCGG ATTCCGGTCG TGGCAGCTGG ACTTCGTGCT CCAGCGGCTC TCATGATAAC
    ATCCAGACCA TGCAGCAGGG CCGCAGCTGG GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG
    GGCAGCAGAG GGAGCTGGGC ATCCGCATGC TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC
    GAGGACTCTG AAGGCGACAC TGGCACCATC AAACGGAGAG GAGGGAGGGA TGTGACCACA
    GATCCAGAGA CCAGCAGCAT CACCTCCACC GGGTCGGAAG AGTCCAGACA GCACAGCCGA
    CGGCCCTCAC CCATCACCGC AGGAAACACC AAGGCTTCCA TCACACGGAA GGACGGCCGC
    TATCGGGACC CTCCACCAAC GCCGCCGGGT TACACCGCGC TGACCATCAC AGATGTGACC
    GAGGGAACGC CACATTCGGG CAGGAAACCA CCAGACTACA CCACCGCCCT GCAGCGCTCG
    CGCCTCGTCA CACACTCCCT CGACTCCCAG CAGCCCCCAG CCGCAGGGAA ACCGGACCCC
    CGGGGCCGAG AGGAGGACGA GGCAGTGGAG GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC
    AAACTCAGAG CTCAGAGGAG GAGAGGTCCA CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214670.1
    CDS300..4856
    Translation

    Target ORF information:

    RefSeq Version XM_017359181.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGTGG TGGACTATAT GGATGAGAAT GAGGAGTATT TCCAGCGGCA GGCATCGCAC 
    CGACAGTCAC GCCGACGCTT TCGCAAGATC AACCAGAAGG GCGAGCGGCA GACCATCATC
    GACACCGTAG ACCCCTATCC TGCTGGCAAG CCCCCCGTGG CGCGGGGATA CCATACGGAA
    TGTGTTAAAG CTCAGCTGCC GGCAGACTTC AGCAGGTTAC ACCTGGCAGA CGGGATTCAC
    CCTCAGGTGA CACACGTGTC CTCCAGTCAT TCAGGATGTA GCATCACCAG TGACTCTGGA
    AGCAGCAGCC TGTCGGACAT TTACCAGGCC ACCGAGAGTG AGCCGGGCGA CATGGACCTC
    AGCGGTCTGC CTGAGACGGC CGTTGACTCC GAGGAGGACG ATGATGAGGA AGACATCGAG
    CGTGCCTCAG ATCCCCTCAT GAGCCGAGAC ATCGTCCGAG ACTGCCTGGA GAAAGACCCC
    ATGGACCGAA CAGATGATGA CATAGAGCAA CTGTTGGAGT TCATGCACCA GCTACCAGCC
    TTTGCCAACA TGACCATGTC TGTCAGGAGG GAGCTGTGTG CTGTCATGGT GTTCGCTGTG
    GTGGAACGGG CCGGCACCAT CGTCCTTAAT GATGGCGAAG AGCTGGACTC GTGGTCTGTG
    ATTCTGAATG GATCGGTGGA AGTGACGCAT CCAGACAGCC GCAATGAAAT CCTCTGCATG
    GGCAACAGCT TCGGCGTTTC TCCCACCATG GAGAAAGAGT ACATGAAGGG GGTGATGAAG
    ACCAAGGTTG ATGACTGCCA GTTTGTGTGC ATCGCTCAGC AGGATTACTG CTGCATCCTC
    AACCAGGTGG AGAAGAACAT GCAGAAGGTG GAGGAGGAAG GAGAGATCGT GATGGTGAAG
    GAGCACAGAG AGCTGGACCG CACAGGCACC AGGAAAGGGC ACATCGTCAT CAAGGGCACA
    TCAGAGCGTC TCACCATGCA CCTTGTAGAA GAGCACTCAG TGGTCGACCC GACTTACATC
    GAGGACTTCC TGCTCACCTA CCGCACCTTC CTGCCGAGCC CCATGGTGGT GGGCAAGAGA
    CTGTTGGAGT GGTTTCATGA CCCCAGCCTC AGGGACAAGG TTACACGGGT AGTCTTGCTG
    TGGGTGAACA ATCACTTTAA TGACTTTGAG GGCGACCCTG CAATGACACA TTTTCTGGAG
    GAGTTCGAGT ACAATCTGGA GAGAGAGAAA ATGTGTGGCC ACCTAAGACT GTTAAACATA
    GCATGTGCTG CCAAAGCTAA GCTGCGTGTG GTGACCCTGA CCAAACCCTC GAGGGAAGCC
    CCGCTGGCCT TCACCCTGCT GGGCGGCTCC GAGAAGGGCT TTCGCATCTT CATCGACAGC
    GTGGAACCCG GCAGTAAAGC TGCAGAGGCC GGCCTTAAAA GAGGAGATCA GATACTGGAG
    GTAAATGGGC AAAACTTTGA AAATGTGCCG CTATCCAAAG CCAACGAGAT TCTCAAAAAC
    AACACGCACC TGTCCATTAC TGTGAAAACC AATCTCTTGG TGTTTAAGGA GCTGCTGGCC
    AGGCCTGACC AGGATCAGGA GCAAGAACAA GAAGAGGAGC CCGACCGGAA AAACGGGGCT
    CCGCACATCC CTAAAATCGG GGACATTAAG AAAGCCAGCC GCTACTCCAT CCCTGACCTG
    GCTGTGGACG TGGAGCAGGT CATGGATCTG GAGAAAGCCA GTAAGAAAGC CAAGGCTAAC
    AGCGCAGGCG GAAGAAACAA GCTGAAGAAG ATCTTCGACA AGACACTGAC CAGTATTCTT
    CCACCCAAAC CTTACAATGA CGTTGGGGTG GGACAGTCCC AGGATGACAG TATTGTGGGC
    CTGAAGCAGT CAAAGCAGAT TCCTCCTGCT CTCCCTGTCA GTGGAAACCT TTCATCAAGC
    AACCCTGACC TGCTGCAGTC ACACCATCGC ATACTCGACT TCAACAACCA GCCTGAAGCG
    GTCCCTATAA ACATGTCAGA CCAGGTATTG CGAGTGTTCA AGGCGGACCA GCAAAGTCGG
    TACATCATGA TTGGGAAGGA CACCACGGCA AAAGAAGTGG TGGCTCAGGC CATCCGAGAG
    TTCGCCCTCA CCGCTGCTGC CGAAGCCTAT TCGTTGTGTG AGGTGTCAGT CACTCCCGAA
    GGAGTCATCA AGCAAAGGAG ACTCCCTGAA CAACTCTCCA AACTGGCTGA CAGGATCCAG
    CTGAGTGGAA GGTACTACCT GAAAAGCAAC ATGGAGACAG AGACACTGTG CTCGGATGAA
    GATGCGCAGG AGTTGCTACG AGAGAGCCAT ATCAGCCTGC TGCAGCTCAG CACGGTGGAA
    GTGGCAACCC AACTCTCCAT GCGCGCCTTT GAGCTGTTCT GTGCCATCGA GCCCACCGAG
    TACATCGATG ACCTGTTTAA GCTGAAAACT CGCCTCACCG GACCCCCCAG CCTCAAGCTT
    TTTGAGGAGG CCATCAATCG GGAGACTTTC TGGGTGGCCA CAGAGGTGGT GCGAGAGGCC
    AACCAGCTCA AACGCATGAA GATTGTCAAG CACTTCATTA AGATTGCACT GCACTGCCGC
    GAATGCAAGA ACTTCAACTC CATGTTTGCC ATCATCAGTG GTCTGAACTT GGCACCAGTG
    TCCAGGCTGA GAGGCACATG GGAGAAGCTG CCCAGCAAAT ACGAGAAGCT GTTTAGCGAT
    CTTCAAGACC TCTTCGATCC CTCCAGGAAT ATGGCCAAGT ATCGCAACCT TCTAAACAGC
    CAGAACCTGC AGCCCCCTAT CATCCCACTC TTCCCCGTAA TCAAGAAGGA TCTCACTTTC
    CTGCATGAAG GGAATGACTC CAAAGTGGAC GGTTTGGTGA ACTTTGAGAA GCTGCGGATG
    ATCGCTAAAG AGATCCGACA CGTGGGCAGA ATGGCAGCCG TAAACATGGA CCCTGCACTG
    ATGTTCAGAA CCAGGAAGAA GAAATGGAGG AGTTTAGGGT CACTCAGCCA GGGCAGTGCC
    AACGCGGCTG TCCTGGATGT GACCCAGACG GGTGGGCACA AGAAGCGGGT CAGAAGGAGT
    TCCTTCCTTA ATGCCAAGAA GCTGTACGAG GACGCACAGA TGGCACGCAA AGTCAAACAA
    TACCTGTCCA ACCTCACCCT GGAGACCAAC GAGGAGAGCC TGCAAACGCT CTCAATGCAG
    TGTGAGCCCT CAATCAACAC CTTGCCGAAG AGTTCTGGGG ACAGAAAGAG ACCCGACACA
    TCACCCGTAG TCTCACGGGC TGCGACCCAC CAGCGCCAGC AGCTTCAAAA AGCCAACCAG
    GCGCTTCAAG TGCCTGCCGT AGCCCTCTAC CCATCCCGCA AAAAAGTGCC AGTCAAAGAC
    TTACCGCCAT TTGGCACAAG CTCACCACAG TCTCTGAAGA AGATCCTGTC CCTTTCAGAG
    GAGGCAGGAG ACAGACACCG AAAGCAGGCA GAGGACAGTG TGTCCAACAA CTCCTCACAG
    CTCTCGTCTC CTCCAACATC TCCTCACAGC TCACCCAAAA AAGCCATCGC ATCAATAAGA
    AGTCATGGTA TGAAGGGCTT TTCCTCCTTG TGTAGTAAAT CACCAACAGG TTATGGACGA
    GCTGGTGATT ACCTGTCCGA CTCTGGCCAC AGTGAGATCT CCTCCCGCTC CAGTCTGGTG
    AGCACTTCTT CTCTGGACAT GGGGCAGGAC GAGCGCCGGC AGCGTTACGG AGGAACGGCC
    GGAGAAGGAC ACGGCAGTGG CCACCGGCTC GAGCGCAGAG CTACTGCTGA CCCTGACCAG
    TACAGCTTGG GGTCCTACTC CTCCATGCAG GACTGTCGTG GTCTGTACGC AGGCGCCACC
    GTCCTGTCCT CACCCAGCTC AGAGGAGCTC ACGCAGGACC AGGGTGACCG GGTGTCCCTG
    GACGCTGCGG ATTCCGGTCG TGGCAGCTGG ACTTCGTGCT CCAGCGGCTC TCATGATAAC
    ATCCAGACCA TGCAGCAGGG CCGCAGCTGG GAGGCGCTGG CAGAGGGCGC AGGCCTGTGG
    GGCAGCAGAG GGAGCTGGGC ATCCGCATGC TCCTCTTCTT CTTCGGTGGC ATGTTGGGGC
    GAGGACTCTG AAGGCGACAC TGGCACCATC AAACGGAGAG GAGGGAGGGA TGTGACCACA
    GATCCAGAGA CCAGCAGCAT CACCTCCACC GGGTCGGAAG AGTCCAGACA GCACAGCCGA
    CGGCCCTCAC CCATCACCGC AGGAAACACC AAGGCTTCCA TCACACGGAA GGACGGCCGC
    TATCGGGACC CTCCACCAAC GCCGCCGGGT TACACCGCGC TGACCATCAC AGATGTGACC
    GAGGGAACGC CACATTCGGG CAGGAAACCA CCAGACTACA CCACCGCCCT GCAGCGCTCG
    CGCCTCGTCA CACACTCCCT CGACTCCCAG CAGCCCCCAG CCGCAGGGAA ACCGGACCCC
    CGGGGCCGAG AGGAGGACGA GGCAGTGGAG GTGGAGGAGG AGGGTGAGTG CTTGTCTCCC
    AAACTCAGAG CTCAGAGGAG GAGAGGTCCA CACACAGCAG TTCCCCCCAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42644
    Clone ID Related Accession (Same CDS sequence) XM_009291280.2 , XM_009291280.3
    Accession Version XM_009291280.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289555.1
    CDS606..5462
    Translation

    Target ORF information:

    RefSeq Version XM_009291280.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291280.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42644
    Clone ID Related Accession (Same CDS sequence) XM_009291280.2 , XM_009291280.3
    Accession Version XM_009291280.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289555.1
    CDS542..5398
    Translation

    Target ORF information:

    RefSeq Version XM_009291280.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291280.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC ACACGGAAGG ACGGCCGCTA TCGGGACCCT
    CCACCAACGC CGCCGGGTTA CACCGCGCTG ACCATCACAG ATGTGACCGA GGGAACGCCA
    CATTCGGGCA GGAAACCACC AGACTACACC ACCGCCCTGC AGCGCTCGCG CCTCGTCACA
    CACTCCCTCG ACTCCCAGCA GCCCCCAGCC GCAGGGAAAC CGGACCCCCG GGGCCGAGAG
    GAGGACGAGG CAGTGGAGGT GGAGGAGGAG GAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42652
    Clone ID Related Accession (Same CDS sequence) XM_009291288.2 , XM_009291288.3
    Accession Version XM_009291288.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC AAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289563.1
    CDS608..5224
    Translation

    Target ORF information:

    RefSeq Version XM_009291288.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC AAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42652
    Clone ID Related Accession (Same CDS sequence) XM_009291288.2 , XM_009291288.3
    Accession Version XM_009291288.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC AAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289563.1
    CDS543..5159
    Translation

    Target ORF information:

    RefSeq Version XM_009291288.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 2 (rapgef2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291288.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTCCT ATGTGGATAA CAGTTTTCGG CAGGCGGTGA TGGTGAATCC GTCAGACAGG 
    ACGCAACAGG ACCTGGAGAT CGTTTATTCA TACCTGCATG GGATGGAGGC TCTGTCCAAC
    CTCCGCGAAC ACCAGCTGAG GATAATGTGT GAAACGGTCC GCTACGAAAG ACACGAAGCC
    AACGAAGTGC TGTATTATCC AGATGACATT GGCACATGCT GGTACATTCT ACTTTCCGGA
    TCGGTTTTCA TCAAAGAGTC CATGTTCTTG CCACGCTCCA GTTTCGGGAA GAGGTCTGCC
    GGGAGTTTGC GTCGTGGATG CGAGTGCATC GTTCTGGAGC CGTCCGAGAT GATTGTGGTG
    GACTATATGG ATGAGAATGA GGAGTATTTC CAGCGGCAGG CATCGCACCG ACAGTCACGC
    CGACGCTTTC GCAAGATCAA CCAGAAGGGC GAGCGGCAGA CCATCATCGA CACCGTAGAC
    CCCTATCCTG CTGGCAAGCC CCCCGTGGCG CGGGGATACC ATACGGAATG TGTTAAAGCT
    CAGCTGCCGG CAGACTTCAG CAGGTTACAC CTGGCAGACG GGATTCACCC TCAGGTGACA
    CACGTGTCCT CCAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG CAGCAGCCTG
    TCGGACATTT ACCAGGCCAC CGAGAGTGAG CCGGGCGACA TGGACCTCAG CGGTCTGCCT
    GAGACGGCCG TTGACTCCGA GGAGGACGAT GATGAGGAAG ACATCGAGCG TGCCTCAGAT
    CCCCTCATGA GCCGAGACAT CGTCCGAGAC TGCCTGGAGA AAGACCCCAT GGACCGAACA
    GATGATGACA TAGAGCAACT GTTGGAGTTC ATGCACCAGC TACCAGCCTT TGCCAACATG
    ACCATGTCTG TCAGGAGGGA GCTGTGTGCT GTCATGGTGT TCGCTGTGGT GGAACGGGCC
    GGCACCATCG TCCTTAATGA TGGCGAAGAG CTGGACTCGT GGTCTGTGAT TCTGAATGGA
    TCGGTGGAAG TGACGCATCC AGACAGCCGC AATGAAATCC TCTGCATGGG CAACAGCTTC
    GGCGTTTCTC CCACCATGGA GAAAGAGTAC ATGAAGGGGG TGATGAAGAC CAAGGTTGAT
    GACTGCCAGT TTGTGTGCAT CGCTCAGCAG GATTACTGCT GCATCCTCAA CCAGGTGGAG
    AAGAACATGC AGAAGGTGGA GGAGGAAGGA GAGATCGTGA TGGTGAAGGA GCACAGAGAG
    CTGGACCGCA CAGGCACCAG GAAAGGGCAC ATCGTCATCA AGGGCACATC AGAGCGTCTC
    ACCATGCACC TTGTAGAAGA GCACTCAGTG GTCGACCCGA CTTACATCGA GGACTTCCTG
    CTCACCTACC GCACCTTCCT GCCGAGCCCC ATGGTGGTGG GCAAGAGACT GTTGGAGTGG
    TTTCATGACC CCAGCCTCAG GGACAAGGTT ACACGGGTAG TCTTGCTGTG GGTGAACAAT
    CACTTTAATG ACTTTGAGGG CGACCCTGCA ATGACACATT TTCTGGAGGA GTTCGAGTAC
    AATCTGGAGA GAGAGAAAAT GTGTGGCCAC CTAAGACTGT TAAACATAGC ATGTGCTGCC
    AAAGCTAAGC TGCGTGTGGT GACCCTGACC AAACCCTCGA GGGAAGCCCC GCTGGCCTTC
    ACCCTGCTGG GCGGCTCCGA GAAGGGCTTT CGCATCTTCA TCGACAGCGT GGAACCCGGC
    AGTAAAGCTG CAGAGGCCGG CCTTAAAAGA GGAGATCAGA TACTGGAGGT AAATGGGCAA
    AACTTTGAAA ATGTGCCGCT ATCCAAAGCC AACGAGATTC TCAAAAACAA CACGCACCTG
    TCCATTACTG TGAAAACCAA TCTCTTGGTG TTTAAGGAGC TGCTGGCCAG GCCTGACCAG
    GATCAGGAGC AAGAACAAGA AGAGGAGCCC GACCGGAAAA ACGGGGCTCC GCACATCCCT
    AAAATCGGGG ACATTAAGAA AGCCAGCCGC TACTCCATCC CTGACCTGGC TGTGGACGTG
    GAGCAGGTCA TGGATCTGGA GAAAGCCAGT AAGAAAGCCA AGGCTAACAG CGCAGGCGGA
    AGAAACAAGC TGAAGAAGAT CTTCGACAAG ACACTGACCA GTATTCTTCC ACCCAAACCT
    TACAATGACG TTGGGGTGGG ACAGTCCCAG GATGACAGTA TTGTGGGCCT GAAGCAGTCA
    AAGCAGATTC CTCCTGCTCT CCCTGTCAGT GGAAACCTTT CATCAAGCAA CCCTGACCTG
    CTGCAGTCAC ACCATCGCAT ACTCGACTTC AACAACCAGC CTGAAGCGGT CCCTATAAAC
    ATGTCAGACC AGGTATTGCG AGTGTTCAAG GCGGACCAGC AAAGTCGGTA CATCATGATT
    GGGAAGGACA CCACGGCAAA AGAAGTGGTG GCTCAGGCCA TCCGAGAGTT CGCCCTCACC
    GCTGCTGCCG AAGCCTATTC GTTGTGTGAG GTGTCAGTCA CTCCCGAAGG AGTCATCAAG
    CAAAGGAGAC TCCCTGAACA ACTCTCCAAA CTGGCTGACA GGATCCAGCT GAGTGGAAGG
    TACTACCTGA AAAGCAACAT GGAGACAGAG ACACTGTGCT CGGATGAAGA TGCGCAGGAG
    TTGCTACGAG AGAGCCATAT CAGCCTGCTG CAGCTCAGCA CGGTGGAAGT GGCAACCCAA
    CTCTCCATGC GCGCCTTTGA GCTGTTCTGT GCCATCGAGC CCACCGAGTA CATCGATGAC
    CTGTTTAAGC TGAAAACTCG CCTCACCGGA CCCCCCAGCC TCAAGCTTTT TGAGGAGGCC
    ATCAATCGGG AGACTTTCTG GGTGGCCACA GAGGTGGTGC GAGAGGCCAA CCAGCTCAAA
    CGCATGAAGA TTGTCAAGCA CTTCATTAAG ATTGCACTGC ACTGCCGCGA ATGCAAGAAC
    TTCAACTCCA TGTTTGCCAT CATCAGTGGT CTGAACTTGG CACCAGTGTC CAGGCTGAGA
    GGCACATGGG AGAAGCTGCC CAGCAAATAC GAGAAGCTGT TTAGCGATCT TCAAGACCTC
    TTCGATCCCT CCAGGAATAT GGCCAAGTAT CGCAACCTTC TAAACAGCCA GAACCTGCAG
    CCCCCTATCA TCCCACTCTT CCCCGTAATC AAGAAGGATC TCACTTTCCT GCATGAAGGG
    AATGACTCCA AAGTGGACGG TTTGGTGAAC TTTGAGAAGC TGCGGATGAT CGCTAAAGAG
    ATCCGACACG TGGGCAGAAT GGCAGCCGTA AACATGGACC CTGCACTGAT GTTCAGAACC
    AGGAAGAAGA AATGGAGGAG TTTAGGGTCA CTCAGCCAGG GCAGTGCCAA CGCGGCTGTC
    CTGGATGTGA CCCAGACGGG TGGGCACAAG AAGCGGGTCA GAAGGAGTTC CTTCCTTAAT
    GCCAAGAAGC TGTACGAGGA CGCACAGATG GCACGCAAAG TCAAACAATA CCTGTCCAAC
    CTCACCCTGG AGACCAACGA GGAGAGCCTG CAAACGCTCT CAATGCAGTG TGAGCCCTCA
    ATCAACACCT TGCCGAAGAG TTCTGGGGAC AGAAAGAGAC CCGACACATC ACCCGTAGTC
    TCACGGGCTG CGACCCACCA GCGCCAGCAG CTTCAAAAAG CCAACCAGGC GCTTCAAGTG
    CCTGCCGTAG CCCTCTACCC ATCCCGCAAA AAAGTGCCAG TCAAAGACTT ACCGCCATTT
    GGCACAAGCT CACCACAGTC TCTGAAGAAG ATCCTGTCCC TTTCAGAGGA GGCAGGAGAC
    AGACACCGAA AGCAGGCAGA GGACAGTGTG TCCAACAACT CCTCACAGCT CTCGTCTCCT
    CCAACATCTC CTCACAGCTC ACCCAAAAAA GCCATCGCAT CAATAAGAAG TCATGGTATG
    AAGGGCTTTT CCTCCTTGTG TAGTAAATCA CCAACAGGTT ATGGACGAGC TGGTGATTAC
    CTGTCCGACT CTGGCCACAG TGAGATCTCC TCCCGCTCCA GTCTGGTGAG CACTTCTTCT
    CTGGACATGG GGCAGGACGA GCGCCGGCAG CGTTACGGAG GAACGGCCGG AGAAGGACAC
    GGCAGTGGCC ACCGGCTCGA GCGCAGAGCT ACTGCTGACC CTGACCAGTA CAGCTTGGGG
    TCCTACTCCT CCATGCAGGA CTGTCGTGGT CTGTACGCAG GCGCCACCGT CCTGTCCTCA
    CCCAGCTCAG AGGAGCTCAC GCAGGACCAG GGTGACCGGG TGTCCCTGGA CGCTGCGGAT
    TCCGGTCGTG GCAGCTGGAC TTCGTGCTCC AGCGGCTCTC ATGATAACAT CCAGACCATG
    CAGCAGGGCC GCAGCTGGGA GGCGCTGGCA GAGGGCGCAG GCCTGTGGGG CAGCAGAGGG
    AGCTGGGCAT CCGCATGCTC CTCTTCTTCT TCGGTGGCAT GTTGGGGCGA GGACTCTGAA
    GGCGACACTG GCACCATCAA ACGGAGAGGA GGGAGGGATG TGACCACAGA TCCAGAGACC
    AGCAGCATCA CCTCCACCGG GTCGGAAGAG TCCAGACAGC ACAGCCGACG GCCCTCACCC
    ATCACCGCAG GAAACACCAA GGCTTCCATC AAGGATGAAC AAGTGTCTGC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.