VHA-A cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	VHA-A
Entrez Gene ID	844228
Full Name	vacuolar ATP synthase subunit A
Synonyms	vacuolar ATP synthase subunit A
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_106539.6	NP_178011.1	vacuolar ATP synthase subunit A
NM_001036222.3	NP_001031299.1	vacuolar ATP synthase subunit A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1A	XP_545103.1
Bos taurus (cattle)	ATP6V1A	NP_776929.1
Mus musculus (house mouse)	Atp6v1a	NP_031534.2
Kluyveromyces lactis	KLLA0F05401g	XP_455325.1
Arabidopsis thaliana (thale cress)	VHA-A	NP_178011.1
Oryza sativa (rice)	Os02g0175400	NP_001046054.1
Homo sapiens (human)	ATP6V1A	NP_001681.2
Danio rerio (zebrafish)	atp6v1ab	XP_002666686.1
Danio rerio (zebrafish)	atp6v1aa	NP_957429.1
Schizosaccharomyces pombe (fission yeast)	vma1	NP_593425.1
Neurospora crassa	NCU01207	XP_961573.1
	ATP6V1A	XP_001106889.1
Gallus gallus (chicken)	ATP6V1A	NP_990305.1
Magnaporthe oryzae (rice blast fungus)	MGG_08087	XP_003715042.1
Pan troglodytes (chimpanzee)	ATP6V1A	XP_003309977.1
	LOC696878	XP_001091043.1
Rattus norvegicus (Norway rat)	Atp6v1a	NP_001101788.1
Caenorhabditis elegans (roundworm)	vha-13	NP_506559.1
Drosophila melanogaster (fruit fly)	Vha68-1	NP_523560.2
Saccharomyces cerevisiae (baker's yeast)	VMA1	NP_010096.1
Oryza sativa (rice)	Os06g0662000	NP_001058280.1
Xenopus tropicalis (tropical clawed frog)	atp6v1a	NP_001007512.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Total VHA-A transcript and protein, together with levels of the truncated variant, were induced by copper. The absence of a genomic sequence representing the truncated variant suggests a RNA editing event causing the production of the truncated VHA-A.
Title: Intracellular localization and induction of a dynamic RNA-editing event of macro-algal V-ATPase subunit A (VHA-A) in response to copper.

oxidative inhibition was unaffected in plants expressing VHA-A C279S and VHA-A C535S, indicating that disulfide bridges involving these cysteine residues are not essential for oxidative inhibition
Title: Regulation of the V-type ATPase by redox modulation.

The following VHA-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VHA-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb31273	NM_001036222.3 Latest version!	Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb31273	NM_106539.6 Latest version!	Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VHA-A ( NM_106539.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_178011.1 Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

Price & Availability↑

CloneID	OAb31273
Clone ID Related Accession (Same CDS sequence)	NM_106539.6 , NM_001036222.3
Accession Version	NM_106539.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	vacuolar ATP synthase subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106539.5.

ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT 
GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT 
GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT 
CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT 
ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC 
ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC 
AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG 
AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC 
ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG 
ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA 
TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC 
CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT 
ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA 
GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG 
CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC 
ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT 
AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA 
TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT 
CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA 
CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT 
TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA 
AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT 
TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA 
AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA 
AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA 
GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC 
GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA 
GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT 
CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG 
GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT 
GAAACTCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_178011.1
CDS	225..2096
Translation	MPAFYGGKLTTFEDDEKESEYGYVRKVSGPVVVADGMAGAAMYELVRVGHDNLIGEIIRLEGDSATIQVYEETAGLTVNDPVLRTHKPLSVELGPGILGNIFDGIQRPLKTIARISGDVYIPRGVSVPALDKDCLWEFQPNKFVEGDTITGGDLYATVFENTLMNHLVALPPDAMGKITYIAPAGQYSLKDTVIELEFQGIKKSYTMLQSWPVRTPRPVASKLAADTPLLTGQRVLDALFPSVLGGTCAIPGAFGCGKTVISQALSKYSNSDAVVYVGCGERGNEMAEVLMDFPQLTMTLPDGREESVMKRTTLVANTSNMPVAAREASIYTGITIAEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLAARLASFYERAGKVKCLGGPERNGSVTIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPSVNWLISYSKYSTALESFYEKFDPDFINIRTKAREVLQREDDLNEIVQLVGKDALAEGDKITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLANQAVERAAGMDGQKITYTLIKHRLGDLFYRLVSQKFEDPAEGEDTLVEKFKKLYDDLNAGFRALEDETR

Target ORF information:

RefSeq Version	NM_106539.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_106539.6

ORF Insert Sequence:

ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT 
GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT 
GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT 
CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT 
ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC 
ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC 
AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG 
AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC 
ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG 
ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA 
TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC 
CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT 
ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA 
GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG 
CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC 
ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT 
AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA 
TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT 
CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA 
CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT 
TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA 
AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT 
TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA 
AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA 
AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA 
GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC 
GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA 
GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT 
CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG 
GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT 
GAAACTCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VHA-A ( NM_001036222.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031299.1 Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

Price & Availability↑

CloneID	OAb31273
Clone ID Related Accession (Same CDS sequence)	NM_106539.6 , NM_001036222.3
Accession Version	NM_001036222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	vacuolar ATP synthase subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001036222.2.

ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT 
GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT 
GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT 
CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT 
ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC 
ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC 
AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG 
AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC 
ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG 
ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA 
TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC 
CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT 
ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA 
GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG 
CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC 
ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT 
AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA 
TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT 
CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA 
CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT 
TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA 
AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT 
TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA 
AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA 
AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA 
GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC 
GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA 
GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT 
CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG 
GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT 
GAAACTCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031299.1
CDS	266..2137
Translation	MPAFYGGKLTTFEDDEKESEYGYVRKVSGPVVVADGMAGAAMYELVRVGHDNLIGEIIRLEGDSATIQVYEETAGLTVNDPVLRTHKPLSVELGPGILGNIFDGIQRPLKTIARISGDVYIPRGVSVPALDKDCLWEFQPNKFVEGDTITGGDLYATVFENTLMNHLVALPPDAMGKITYIAPAGQYSLKDTVIELEFQGIKKSYTMLQSWPVRTPRPVASKLAADTPLLTGQRVLDALFPSVLGGTCAIPGAFGCGKTVISQALSKYSNSDAVVYVGCGERGNEMAEVLMDFPQLTMTLPDGREESVMKRTTLVANTSNMPVAAREASIYTGITIAEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLAARLASFYERAGKVKCLGGPERNGSVTIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPSVNWLISYSKYSTALESFYEKFDPDFINIRTKAREVLQREDDLNEIVQLVGKDALAEGDKITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLANQAVERAAGMDGQKITYTLIKHRLGDLFYRLVSQKFEDPAEGEDTLVEKFKKLYDDLNAGFRALEDETR

Target ORF information:

RefSeq Version	NM_001036222.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036222.3

ORF Insert Sequence:

ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT 
GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT 
GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT 
CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT 
ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC 
ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC 
AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG 
AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC 
ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG 
ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA 
TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC 
CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT 
ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA 
GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG 
CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC 
ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT 
AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA 
TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT 
CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA 
CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT 
TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA 
AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT 
TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA 
AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA 
AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA 
GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC 
GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA 
GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT 
CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG 
GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT 
GAAACTCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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VHA-A cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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