Atp6v1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v1a
Entrez Gene ID	685232
Full Name	ATPase H+ transporting V1 subunit A
Synonyms	Atp6v1a1
General protein information	Preferred Names ATPase H+ transporting V1 subunit A Names V-type proton ATPase catalytic subunit A ATPase, H+ transporting, lysosomal V1 subunit A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006248348.3	XP_006248410.1	V-type proton ATPase catalytic subunit A isoform X1
XM_006248349.3	XP_006248411.1	V-type proton ATPase catalytic subunit A isoform X1
NM_001108318.2	NP_001101788.1	V-type proton ATPase catalytic subunit A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1A	XP_545103.1
Bos taurus (cattle)	ATP6V1A	NP_776929.1
Mus musculus (house mouse)	Atp6v1a	NP_031534.2
Kluyveromyces lactis	KLLA0F05401g	XP_455325.1
Arabidopsis thaliana (thale cress)	VHA-A	NP_178011.1
Oryza sativa (rice)	Os02g0175400	NP_001046054.1
Homo sapiens (human)	ATP6V1A	NP_001681.2
Danio rerio (zebrafish)	atp6v1ab	XP_002666686.1
Danio rerio (zebrafish)	atp6v1aa	NP_957429.1
Schizosaccharomyces pombe (fission yeast)	vma1	NP_593425.1
Neurospora crassa	NCU01207	XP_961573.1
	ATP6V1A	XP_001106889.1
Gallus gallus (chicken)	ATP6V1A	NP_990305.1
Magnaporthe oryzae (rice blast fungus)	MGG_08087	XP_003715042.1
Pan troglodytes (chimpanzee)	ATP6V1A	XP_003309977.1
	LOC696878	XP_001091043.1
Rattus norvegicus (Norway rat)	Atp6v1a	NP_001101788.1
Caenorhabditis elegans (roundworm)	vha-13	NP_506559.1
Drosophila melanogaster (fruit fly)	Vha68-1	NP_523560.2
Saccharomyces cerevisiae (baker's yeast)	VMA1	NP_010096.1
Oryza sativa (rice)	Os06g0662000	NP_001058280.1
Xenopus tropicalis (tropical clawed frog)	atp6v1a	NP_001007512.1

Gene Ontology
Bibliography

Related articles in PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife6(2017 03)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.
Chintagari NR, Mishra A, Su L, Wang Y, Ayalew S, Hartson SD, Liu L
PloS one5(2)e9228(2010 Feb)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that silencing of V-ATPase a1 and B2 subunits decreased stimulated surfactant secretion.
Title: Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.

The following Atp6v1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07129	NM_001108318.2 Latest version!	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa07129	XM_006248349.3 Latest version!	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa07129	XM_006248348.3 Latest version!	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v1a ( NM_001108318.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101788.1 Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), mRNA.

Price & Availability↑

CloneID	ORa07129
Clone ID Related Accession (Same CDS sequence)	XM_006248348.3 , XM_006248349.3 , NM_001108318.2
Accession Version	NM_001108318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase catalytic subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034274.1. On Sep 10, 2008 this sequence version replaced NM_001108318.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FQ211993.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101788.1
CDS	106..1959
Translation	MDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWEFIPSKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRSRGSVTYIAPPGNYDASDVVLELEFEGVKEKLSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMISFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	NM_001108318.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108318.2

ORF Insert Sequence:

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp6v1a ( XM_006248348.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248410.1 Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07129
Clone ID Related Accession (Same CDS sequence)	XM_006248348.3 , XM_006248349.3 , NM_001108318.2
Accession Version	XM_006248348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248348.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248410.1
CDS	139..1992
Translation	MDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWEFIPSKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRSRGSVTYIAPPGNYDASDVVLELEFEGVKEKLSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMISFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	XM_006248348.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248348.3

ORF Insert Sequence:

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp6v1a ( XM_006248349.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248411.1 Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07129
Clone ID Related Accession (Same CDS sequence)	XM_006248348.3 , XM_006248349.3 , NM_001108318.2
Accession Version	XM_006248349.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248349.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248411.1
CDS	303..2156
Translation	MDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWEFIPSKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRSRGSVTYIAPPGNYDASDVVLELEFEGVKEKLSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMISFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	XM_006248349.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+ transporting V1 subunit A (Atp6v1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248349.3

ORF Insert Sequence:

ATGGATTTCT CCAAGCTACC CAAAATCCGT GATGAAGACA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGAG TCTCAGGGCC TGTGGTCACC GCCTGTGACA TGGCGGGCGC TGCCATGTAC 
GAGCTGGTGA GAGTGGGGCA CAGCGAGCTG GTCGGAGAGA TTATCCGACT GGAAGGTGAC 
ATGGCCACCA TTCAGGTGTA CGAGGAAACT TCTGGTGTCT CTGTTGGAGA CCCTGTACTC 
CGCACTGGTA AACCTCTCTC GGTAGAACTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAGA GACCTCTATC GGATATCAGC AGTCAGACCC AAAGTATCTA CATCCCCAGA 
GGAGTAAATG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG AGTTTATACC CAGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGTGGA GATATTTATG GGATTGTCAA TGAGAACTCG 
CTCATCAAAC ACAAAATCAT GCTACCCCCA CGCAGCCGAG GAAGTGTGAC TTACATCGCA 
CCGCCTGGGA ACTACGATGC CTCTGATGTC GTCCTGGAGC TTGAATTTGA AGGTGTGAAG 
GAGAAGTTGA GCATGGTCCA AGTGTGGCCT GTGCGTCAGG TGCGGCCTGT CACTGAAAAG 
CTGCCAGCCA ACCACCCCTT GCTTACTGGC CAGAGAGTGC TCGATGCCCT TTTCCCGTGT 
GTTCAGGGAG GAACTACTGC TATTCCAGGT GCTTTTGGCT GTGGGAAGAC TGTGATTTCC 
CAGTCTCTAT CCAAGTACTC CAACAGCGAT GTGATTATCT ATGTCGGCTG CGGTGAAAGA 
GGGAATGAGA TGTCAGAAGT TCTCCGGGAC TTCCCTGAGC TCACTATGGA AGTTGATGGT 
AAAGTAGAGT CCATCATGAA GAGGACAGCG CTGGTAGCCA ATACCTCCAA TATGCCTGTG 
GCTGCAAGAG AGGCCTCCAT TTACACTGGA ATTACACTAT CAGAATACTT CCGTGACATG 
GGGTACCACG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTCAGAGAA 
ATCTCTGGTC GCTTAGCTGA AATGCCTGCA GATAGTGGAT ACCCTGCATA CCTTGGTGCC 
CGACTGGCTT CTTTCTATGA GCGAGCAGGC AGAGTGAAAT GTCTTGGAAA CCCTGAGAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTCACATCTG CTACTCTGGG TATTGTTCAG GTGTTCTGGG GCTTGGATAA GAAGCTAGCT 
CAGCGCAAGC ACTTCCCGTC CGTCAACTGG CTCATTAGCT ACAGCAAGTA CATGCGCGCC 
CTGGACGAGT ACTATGACAA ACACTTCACA GAGTTCGTGC CTCTGAGGAC CAAAGCTAAG 
GAGATTCTGC AGGAAGAGGA GGATCTGGCG GAAATCGTGC AGCTCGTGGG AAAGGCGTCT 
TTAGCAGAGA CAGATAAAAT CACCCTGGAG GTAGCAAAAC TTATCAAAGA TGACTTCCTA 
CAACAAAATG GGTACACTCC TTATGACAGG TTCTGTCCAT TCTATAAGAC GGTGGGGATG 
CTGTCCAACA TGATTTCATT CTATGATATG GCCCGCCGGG CTGTGGAGAC CACCGCCCAG 
AGTGACAATA AGATCACATG GTCCATTATC CGTGAGCACA TGGGGGAGAT TCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCAGTG AAGGATGGCG AGGCAAAGAT CAAGGCCGAC 
TACGCACAGC TTCTTGAAGA TATGCAGAAC GCATTCCGTA GCCTGGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
eLife6(2017 03)
Miles AL,Burr SP,Grice GL,Nathan JA

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.
PloS one5(2)e9228(2010 Feb)
Chintagari NR,Mishra A,Su L,Wang Y,Ayalew S,Hartson SD,Liu L

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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Atp6v1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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