Vha68-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha68-1
Entrez Gene ID	45668
Full Name	Vacuolar H[+] ATPase 68kD subunit 1
Synonyms	A subunit,ATP6V1A,CG12403,Dmel\CG12403,Vha,Vha-68-1,Vha68,VhaA,vha67-1,vha68-1
General protein information	Preferred Names Vacuolar H[+] ATPase 68kD subunit 1 Names CG12403 gene product from transcript CG12403-RA CG12403-PA CG12403-PB Vha68-1-PA Vha68-1-PB vacuolar ATPase subunit A vacuolar H[+] ATPase 68kD subunit 1 vacuolar H[+] ATPase subunit 68-1 vacuolar H[+]-ATPase 68 vacuolar H[+]-ATPase 68kD A subunit vacuolar H[+]-ATPase A subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34B-34A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_078836.3	NP_523560.2	vacuolar H[+] ATPase 68kD subunit 1, isoform A
NM_001273497.1	NP_001260426.1	vacuolar H[+] ATPase 68kD subunit 1, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1A	XP_545103.1
Bos taurus (cattle)	ATP6V1A	NP_776929.1
Mus musculus (house mouse)	Atp6v1a	NP_031534.2
Kluyveromyces lactis	KLLA0F05401g	XP_455325.1
Arabidopsis thaliana (thale cress)	VHA-A	NP_178011.1
Oryza sativa (rice)	Os02g0175400	NP_001046054.1
Homo sapiens (human)	ATP6V1A	NP_001681.2
Danio rerio (zebrafish)	atp6v1ab	XP_002666686.1
Danio rerio (zebrafish)	atp6v1aa	NP_957429.1
Schizosaccharomyces pombe (fission yeast)	vma1	NP_593425.1
Neurospora crassa	NCU01207	XP_961573.1
	ATP6V1A	XP_001106889.1
Gallus gallus (chicken)	ATP6V1A	NP_990305.1
Magnaporthe oryzae (rice blast fungus)	MGG_08087	XP_003715042.1
Pan troglodytes (chimpanzee)	ATP6V1A	XP_003309977.1
	LOC696878	XP_001091043.1
Rattus norvegicus (Norway rat)	Atp6v1a	NP_001101788.1
Caenorhabditis elegans (roundworm)	vha-13	NP_506559.1
Drosophila melanogaster (fruit fly)	Vha68-1	NP_523560.2
Saccharomyces cerevisiae (baker's yeast)	VMA1	NP_010096.1
Oryza sativa (rice)	Os06g0662000	NP_001058280.1
Xenopus tropicalis (tropical clawed frog)	atp6v1a	NP_001007512.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha68-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha68-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15222	NM_001273497.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant B (Vha68-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15222	NM_078836.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant A (Vha68-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha68-1 ( NM_078836.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523560.2 Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant A (Vha68-1), mRNA.

Price & Availability↑

CloneID	OFa15222
Clone ID Related Accession (Same CDS sequence)	NM_078836.3 , NM_001273497.1
Accession Version	NM_078836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68kD subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078836.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACT TGAGGAAATT CAAAGACGAG GAGCGCGAGT CGGAATATGG CCGTGTCTAC 
GCGGTATCCG GACCAGTGGT CACCGCTGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG CGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTGACTGTCG GCGATCCGGT GCTGCGTACC 
GGCAAACCTC TTTCCGTGGA ACTTGGACCC GGCATTATGG GCAGCATCTT CGACGGCATC 
CAACGTCCTT TGCGGGACAT TGGTGTCATG ACCAACTCCA TCTATATACC CAAAGGTGTC 
AACACAACTG CTTTGTCGCG CTCGGAGATG TGGGAATTTA ATCCGCTGAA TGTGCGGGTG 
GGATCCCACA TCACCGGAGG AGATCTGTAT GGAGTGGTAC ACGAGAACAC GCTGGTGAAG 
CAGCGCATGA TTGTGGCACC CAGGGCTAAG GGAACCGTTC GATACATTGC CCCCGCGGGC 
AACTACAACC TGGAGGACAT TGTCCTGGAG ACGGAGTTCG ACGGCGAGAT AACCAAGCAC 
ACCATGTTGC AGGTCTGGCC AGTGCGGCAG CCACGTCCCG TCACAGAGAA GCTGCCAGCC 
AACCATCCGC TCTTCACGGG CCAACGCGTC CTTGACTCGC TCTTCCCCTG CGTACAGGGC 
GGCACCACTG CCATCCCCGG TGCCTTTGGC TGCGGCAAGA CCGTCATTTC GCAGGCCCTG 
TCCAAGTACT CCAACTCTGA TGTGATCATC TACGTCGGTT GCGGCGAGCG CGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTTCCCGAA CTGACCTGCG AGATAGATGG CGTCACCGAG 
TCCATTATGA AGCGAACTGC TCTGGTGGCC AACACCTCCA ACATGCCGGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG AATCACTCTG TCTGAATACT TCCGTGATAT GGGCTACAAC 
GTAGCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CACTTCGTGA GATTTCGGGT 
CGTTTGGCTG AGATGCCTGC CGATTCTGGC TACCCGGCTT ATCTAGGAGC TCGTCTGGCC 
ACATTCTACG AGCGTGCTGG GCGCGTCAAG TGCTTGGGTA ACCCGGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTTTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAATTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAA 
TACTATGACA AGAACTACCC CGAGTTCGTG CCACTACGCA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTT CAGCTGGTGG GCAAAGCATC ACTGGCCGAG 
ACCGACAAGG TGACCCTGGA AGTGGCAAAG CTGCTGAAGG ACGACTTTCT GCAACAGAAC 
TCCTACTCAC CATACGATCG CGTTTGTCCC TTCTACAAGA CCGTGGGCAT GCTGAGAAAC 
ATCATGGCCT TCTATGAGAC CGCCCGTCAT GCCGTTGAGT CCACAGCCCA GTCGGACAAC 
AAGATCACAT GGAACACCAT CAGGGAATCG ATGGGCGGAA TTATGTACCA GCTGTCGTCA 
ATGAAGTTCA AGGACCCGGT GAAAGATGGC GAGCAAAAGA TCAAGGCGGA CTACGACCAG 
CTGTACGAGG ATCTGCAGCA GGCCTTCCGA AATCTAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523560.2
CDS	124..1968
Translation	MSNLRKFKDEERESEYGRVYAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLRDIGVMTNSIYIPKGVNTTALSRSEMWEFNPLNVRVGSHITGGDLYGVVHENTLVKQRMIVAPRAKGTVRYIAPAGNYNLEDIVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLFTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTCEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVAMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLATFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDEYYDKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKVTLEVAKLLKDDFLQQNSYSPYDRVCPFYKTVGMLRNIMAFYETARHAVESTAQSDNKITWNTIRESMGGIMYQLSSMKFKDPVKDGEQKIKADYDQLYEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_078836.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant A (Vha68-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078836.3

ORF Insert Sequence:

ATGTCCAACT TGAGGAAATT CAAAGACGAG GAGCGCGAGT CGGAATATGG CCGTGTCTAC 
GCGGTATCCG GACCAGTGGT CACCGCTGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG CGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTGACTGTCG GCGATCCGGT GCTGCGTACC 
GGCAAACCTC TTTCCGTGGA ACTTGGACCC GGCATTATGG GCAGCATCTT CGACGGCATC 
CAACGTCCTT TGCGGGACAT TGGTGTCATG ACCAACTCCA TCTATATACC CAAAGGTGTC 
AACACAACTG CTTTGTCGCG CTCGGAGATG TGGGAATTTA ATCCGCTGAA TGTGCGGGTG 
GGATCCCACA TCACCGGAGG AGATCTGTAT GGAGTGGTAC ACGAGAACAC GCTGGTGAAG 
CAGCGCATGA TTGTGGCACC CAGGGCTAAG GGAACCGTTC GATACATTGC CCCCGCGGGC 
AACTACAACC TGGAGGACAT TGTCCTGGAG ACGGAGTTCG ACGGCGAGAT AACCAAGCAC 
ACCATGTTGC AGGTCTGGCC AGTGCGGCAG CCACGTCCCG TCACAGAGAA GCTGCCAGCC 
AACCATCCGC TCTTCACGGG CCAACGCGTC CTTGACTCGC TCTTCCCCTG CGTACAGGGC 
GGCACCACTG CCATCCCCGG TGCCTTTGGC TGCGGCAAGA CCGTCATTTC GCAGGCCCTG 
TCCAAGTACT CCAACTCTGA TGTGATCATC TACGTCGGTT GCGGCGAGCG CGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTTCCCGAA CTGACCTGCG AGATAGATGG CGTCACCGAG 
TCCATTATGA AGCGAACTGC TCTGGTGGCC AACACCTCCA ACATGCCGGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG AATCACTCTG TCTGAATACT TCCGTGATAT GGGCTACAAC 
GTAGCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CACTTCGTGA GATTTCGGGT 
CGTTTGGCTG AGATGCCTGC CGATTCTGGC TACCCGGCTT ATCTAGGAGC TCGTCTGGCC 
ACATTCTACG AGCGTGCTGG GCGCGTCAAG TGCTTGGGTA ACCCGGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTTTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAATTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAA 
TACTATGACA AGAACTACCC CGAGTTCGTG CCACTACGCA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTT CAGCTGGTGG GCAAAGCATC ACTGGCCGAG 
ACCGACAAGG TGACCCTGGA AGTGGCAAAG CTGCTGAAGG ACGACTTTCT GCAACAGAAC 
TCCTACTCAC CATACGATCG CGTTTGTCCC TTCTACAAGA CCGTGGGCAT GCTGAGAAAC 
ATCATGGCCT TCTATGAGAC CGCCCGTCAT GCCGTTGAGT CCACAGCCCA GTCGGACAAC 
AAGATCACAT GGAACACCAT CAGGGAATCG ATGGGCGGAA TTATGTACCA GCTGTCGTCA 
ATGAAGTTCA AGGACCCGGT GAAAGATGGC GAGCAAAAGA TCAAGGCGGA CTACGACCAG 
CTGTACGAGG ATCTGCAGCA GGCCTTCCGA AATCTAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-1 ( NM_001273497.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260426.1 Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant B (Vha68-1), mRNA.

Price & Availability↑

CloneID	OFa15222
Clone ID Related Accession (Same CDS sequence)	NM_078836.3 , NM_001273497.1
Accession Version	NM_001273497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68kD subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACT TGAGGAAATT CAAAGACGAG GAGCGCGAGT CGGAATATGG CCGTGTCTAC 
GCGGTATCCG GACCAGTGGT CACCGCTGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG CGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTGACTGTCG GCGATCCGGT GCTGCGTACC 
GGCAAACCTC TTTCCGTGGA ACTTGGACCC GGCATTATGG GCAGCATCTT CGACGGCATC 
CAACGTCCTT TGCGGGACAT TGGTGTCATG ACCAACTCCA TCTATATACC CAAAGGTGTC 
AACACAACTG CTTTGTCGCG CTCGGAGATG TGGGAATTTA ATCCGCTGAA TGTGCGGGTG 
GGATCCCACA TCACCGGAGG AGATCTGTAT GGAGTGGTAC ACGAGAACAC GCTGGTGAAG 
CAGCGCATGA TTGTGGCACC CAGGGCTAAG GGAACCGTTC GATACATTGC CCCCGCGGGC 
AACTACAACC TGGAGGACAT TGTCCTGGAG ACGGAGTTCG ACGGCGAGAT AACCAAGCAC 
ACCATGTTGC AGGTCTGGCC AGTGCGGCAG CCACGTCCCG TCACAGAGAA GCTGCCAGCC 
AACCATCCGC TCTTCACGGG CCAACGCGTC CTTGACTCGC TCTTCCCCTG CGTACAGGGC 
GGCACCACTG CCATCCCCGG TGCCTTTGGC TGCGGCAAGA CCGTCATTTC GCAGGCCCTG 
TCCAAGTACT CCAACTCTGA TGTGATCATC TACGTCGGTT GCGGCGAGCG CGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTTCCCGAA CTGACCTGCG AGATAGATGG CGTCACCGAG 
TCCATTATGA AGCGAACTGC TCTGGTGGCC AACACCTCCA ACATGCCGGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG AATCACTCTG TCTGAATACT TCCGTGATAT GGGCTACAAC 
GTAGCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CACTTCGTGA GATTTCGGGT 
CGTTTGGCTG AGATGCCTGC CGATTCTGGC TACCCGGCTT ATCTAGGAGC TCGTCTGGCC 
ACATTCTACG AGCGTGCTGG GCGCGTCAAG TGCTTGGGTA ACCCGGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTTTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAATTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAA 
TACTATGACA AGAACTACCC CGAGTTCGTG CCACTACGCA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTT CAGCTGGTGG GCAAAGCATC ACTGGCCGAG 
ACCGACAAGG TGACCCTGGA AGTGGCAAAG CTGCTGAAGG ACGACTTTCT GCAACAGAAC 
TCCTACTCAC CATACGATCG CGTTTGTCCC TTCTACAAGA CCGTGGGCAT GCTGAGAAAC 
ATCATGGCCT TCTATGAGAC CGCCCGTCAT GCCGTTGAGT CCACAGCCCA GTCGGACAAC 
AAGATCACAT GGAACACCAT CAGGGAATCG ATGGGCGGAA TTATGTACCA GCTGTCGTCA 
ATGAAGTTCA AGGACCCGGT GAAAGATGGC GAGCAAAAGA TCAAGGCGGA CTACGACCAG 
CTGTACGAGG ATCTGCAGCA GGCCTTCCGA AATCTAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260426.1
CDS	191..2035
Translation	MSNLRKFKDEERESEYGRVYAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLRDIGVMTNSIYIPKGVNTTALSRSEMWEFNPLNVRVGSHITGGDLYGVVHENTLVKQRMIVAPRAKGTVRYIAPAGNYNLEDIVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLFTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTCEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVAMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLATFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDEYYDKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKVTLEVAKLLKDDFLQQNSYSPYDRVCPFYKTVGMLRNIMAFYETARHAVESTAQSDNKITWNTIRESMGGIMYQLSSMKFKDPVKDGEQKIKADYDQLYEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_001273497.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 1, transcript variant B (Vha68-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273497.1

ORF Insert Sequence:

ATGTCCAACT TGAGGAAATT CAAAGACGAG GAGCGCGAGT CGGAATATGG CCGTGTCTAC 
GCGGTATCCG GACCAGTGGT CACCGCTGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG CGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTGACTGTCG GCGATCCGGT GCTGCGTACC 
GGCAAACCTC TTTCCGTGGA ACTTGGACCC GGCATTATGG GCAGCATCTT CGACGGCATC 
CAACGTCCTT TGCGGGACAT TGGTGTCATG ACCAACTCCA TCTATATACC CAAAGGTGTC 
AACACAACTG CTTTGTCGCG CTCGGAGATG TGGGAATTTA ATCCGCTGAA TGTGCGGGTG 
GGATCCCACA TCACCGGAGG AGATCTGTAT GGAGTGGTAC ACGAGAACAC GCTGGTGAAG 
CAGCGCATGA TTGTGGCACC CAGGGCTAAG GGAACCGTTC GATACATTGC CCCCGCGGGC 
AACTACAACC TGGAGGACAT TGTCCTGGAG ACGGAGTTCG ACGGCGAGAT AACCAAGCAC 
ACCATGTTGC AGGTCTGGCC AGTGCGGCAG CCACGTCCCG TCACAGAGAA GCTGCCAGCC 
AACCATCCGC TCTTCACGGG CCAACGCGTC CTTGACTCGC TCTTCCCCTG CGTACAGGGC 
GGCACCACTG CCATCCCCGG TGCCTTTGGC TGCGGCAAGA CCGTCATTTC GCAGGCCCTG 
TCCAAGTACT CCAACTCTGA TGTGATCATC TACGTCGGTT GCGGCGAGCG CGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTTCCCGAA CTGACCTGCG AGATAGATGG CGTCACCGAG 
TCCATTATGA AGCGAACTGC TCTGGTGGCC AACACCTCCA ACATGCCGGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG AATCACTCTG TCTGAATACT TCCGTGATAT GGGCTACAAC 
GTAGCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CACTTCGTGA GATTTCGGGT 
CGTTTGGCTG AGATGCCTGC CGATTCTGGC TACCCGGCTT ATCTAGGAGC TCGTCTGGCC 
ACATTCTACG AGCGTGCTGG GCGCGTCAAG TGCTTGGGTA ACCCGGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTTTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAATTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAA 
TACTATGACA AGAACTACCC CGAGTTCGTG CCACTACGCA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTT CAGCTGGTGG GCAAAGCATC ACTGGCCGAG 
ACCGACAAGG TGACCCTGGA AGTGGCAAAG CTGCTGAAGG ACGACTTTCT GCAACAGAAC 
TCCTACTCAC CATACGATCG CGTTTGTCCC TTCTACAAGA CCGTGGGCAT GCTGAGAAAC 
ATCATGGCCT TCTATGAGAC CGCCCGTCAT GCCGTTGAGT CCACAGCCCA GTCGGACAAC 
AAGATCACAT GGAACACCAT CAGGGAATCG ATGGGCGGAA TTATGTACCA GCTGTCGTCA 
ATGAAGTTCA AGGACCCGGT GAAAGATGGC GAGCAAAAGA TCAAGGCGGA CTACGACCAG 
CTGTACGAGG ATCTGCAGCA GGCCTTCCGA AATCTAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha68-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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