ATP6V1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V1A
Entrez Gene ID	523
Full Name	ATPase H+ transporting V1 subunit A
Synonyms	ARCL2D,ATP6A1,ATP6V1A1,HO68,VA68,VPP2,Vma1
General protein information	Preferred Names ATPase H+ transporting V1 subunit A Names V-type proton ATPase catalytic subunit A ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A ATPase, H+ transporting, lysosomal, subunit A1 H(+)-transporting two-sector ATPase, subunit A H+-transporting ATPase chain A, vacuolar (VA68 type) V-ATPase 69 kDa subunit 1 V-ATPase A subunit 1 V-ATPase subunit A vacuolar ATP synthase catalytic subunit A, ubiquitous isoform vacuolar ATPase isoform VA68 vacuolar proton pump alpha subunit 1 vacuolar proton pump subunit alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.31
Summary	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008].
Disorder MIM:	607027
Disorder Html:	Cutis laxa, autosomal recessive, type IID, 617403 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001690.3	NP_001681.2	V-type proton ATPase catalytic subunit A
NM_001690.4	NP_001681.2	V-type proton ATPase catalytic subunit A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1A	XP_545103.1
Bos taurus (cattle)	ATP6V1A	NP_776929.1
Mus musculus (house mouse)	Atp6v1a	NP_031534.2
Kluyveromyces lactis	KLLA0F05401g	XP_455325.1
Arabidopsis thaliana (thale cress)	VHA-A	NP_178011.1
Oryza sativa (rice)	Os02g0175400	NP_001046054.1
Homo sapiens (human)	ATP6V1A	NP_001681.2
Danio rerio (zebrafish)	atp6v1ab	XP_002666686.1
Danio rerio (zebrafish)	atp6v1aa	NP_957429.1
Schizosaccharomyces pombe (fission yeast)	vma1	NP_593425.1
Neurospora crassa	NCU01207	XP_961573.1
	ATP6V1A	XP_001106889.1
Gallus gallus (chicken)	ATP6V1A	NP_990305.1
Magnaporthe oryzae (rice blast fungus)	MGG_08087	XP_003715042.1
Pan troglodytes (chimpanzee)	ATP6V1A	XP_003309977.1
	LOC696878	XP_001091043.1
Rattus norvegicus (Norway rat)	Atp6v1a	NP_001101788.1
Caenorhabditis elegans (roundworm)	vha-13	NP_506559.1
Drosophila melanogaster (fruit fly)	Vha68-1	NP_523560.2
Saccharomyces cerevisiae (baker's yeast)	VMA1	NP_010096.1
Oryza sativa (rice)	Os06g0662000	NP_001058280.1
Xenopus tropicalis (tropical clawed frog)	atp6v1a	NP_001007512.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and Transcriptional Regulation of Human ATP6V1A Gene in Gastric Cancers.
Wang P, Wang L, Sha J, Lou G, Lu N, Hang B, Mao JH, Zou X
Scientific reports7(1)3015(2017 Jun)

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife6(2017 03)

Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.
Van Damme T, Gardeitchik T, Mohamed M, Guerrero-Castillo S, Freisinger P, Guillemyn B, Kariminejad A, Dalloyaux D, van Kraaij S, Lefeber DJ, Syx D, Steyaert W, De Rycke R, Hoischen A, Kamsteeg EJ, Wong SY, van Scherpenzeel M, Jamali P, Brandt U, Nijtmans L, Korenke GC, Chung BHY, Mak CCY, Hausser I, Kornak U, Fischer-Zirnsak B, Strom TM, Meitinger T, Alanay Y, Utine GE, Leung PKC, Ghaderi-Sohi S, Coucke P, Symoens S, De Paepe A, Thiel C, Haack TB, Malfait F, Morava E, Callewaert B, Wevers RA
American journal of human genetics100(2)216-227(2017 Feb)

Expression and role of V1A subunit of V-ATPases in gastric cancer cells.
Liu P, Chen H, Han L, Zou X, Shen W
International journal of clinical oncology20(4)725-35(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report biallelic mutations in ATP6V1E1 and ATP6V1A, respectively encoding the E1 and A subunits of the V1 domain of V-ATPase, as a cause of distinct metabolic and multisystemic cutis laxa entities.
Title: Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.

V1A subunit of V-ATPase has a role in the progression and prognosis of gastric cancer.
Title: Expression and role of V1A subunit of V-ATPases in gastric cancer cells.

Data show that the cAMP/PKA/CREB signaling pathway initiates acidosis-induced V-ATPase trafficking in salivary ducts via regulation of Rab11b expression.
Title: Acidosis-induced V-ATPase trafficking in salivary ducts is initiated by cAMP/PKA/CREB pathway via regulation of Rab11b expression.

These data introduce Rab11b as a crucial regulator and Rip11 as mediator of acidosis-induced V-ATPase traffic in duct cells of submandibular gland.
Title: Rab11b and its effector Rip11 regulate the acidosis-induced traffic of V-ATPase in salivary ducts.

This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia.
Title: Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation.

The following ATP6V1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55807	NM_001690.4 Latest version!	Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu55807	NM_001690.3	Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V1A ( NM_001690.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001681.2 Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Price & Availability↑

CloneID	OHu55807
Clone ID Related Accession (Same CDS sequence)	NM_001690.3 , NM_001690.4
Accession Version	NM_001690.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	V-type proton ATPase catalytic subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC350361.1, AK314779.1, AF339827.1, BC012169.1 and CA435314.1. This sequence is a reference standard in the RefSeqGene project. On Nov 13, 2010 this sequence version replaced NM_001690.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.46685.1, SRR1660803.97715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTTT CCAAGCTACC CAAAATACTC GATGAAGATA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGGG TCTCAGGACC TGTGGTTACA GCCTGTGACA TGGCGGGTGC AGCCATGTAT 
GAGCTGGTGA GAGTGGGCCA CAGCGAATTG GTTGGAGAGA TTATTCGATT GGAGGGTGAC 
ATGGCTACTA TTCAGGTGTA TGAAGAAACT TCTGGTGTGT CTGTTGGAGA TCCTGTACTT 
CGCACTGGTA AACCCCTCTC TGTAGAGCTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAAA GACCTTTGTC GGATATCAGC AGTCAGACCC AAAGCATCTA CATCCCCAGA 
GGAGTAAACG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG ACTTTACACC TTGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGCGGA GACATTTATG GAATTGTCAG TGAGAACTCG 
CTTATCAAAC ACAAAATCAT GTTACCCCCA CGAAACAGAG GAACTGTAAC TTACATTGCT 
CCACCTGGGA ATTATGATAC CTCTGATGTT GTCTTGGAGC TTGAATTTGA AGGTGTAAAG 
GAGAAGTTCA CCATGGTGCA AGTATGGCCT GTACGTCAAG TTCGACCTGT CACTGAGAAG 
CTGCCAGCCA ATCATCCTCT GTTGACTGGC CAGAGAGTCC TTGATGCCCT TTTTCCGTGT 
GTCCAGGGAG GAACTACTGC TATCCCTGGA GCCTTTGGCT GTGGAAAGAC AGTGATATCA 
CAGTCTCTAT CCAAGTATTC TAACAGTGAT GTAATCATCT ATGTAGGATG TGGTGAAAGA 
GGAAATGAGA TGTCTGAAGT CCTCCGGGAC TTCCCAGAGC TCACAATGGA GGTTGATGGT 
AAGGTAGAGT CAATTATGAA GAGGACAGCT TTGGTAGCCA ATACCTCCAA TATGCCTGTT 
GCTGCTAGAG AAGCCTCTAT TTATACTGGA ATCACACTGT CAGAGTACTT CCGTGACATG 
GGCTATCATG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTTAGAGAA 
ATCTCTGGTC GTTTAGCTGA AATGCCTGCA GATAGTGGAT ATCCAGCCTA TCTTGGTGCC 
CGTCTGGCCT CGTTTTATGA ACGAGCAGGC AGGGTGAAAT GTCTTGGAAA TCCTGAAAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTTACATCTG CCACTCTTGG TATCGTTCAG GTGTTCTGGG GCTTAGATAA GAAACTAGCT 
CAACGTAAGC ATTTCCCCTC TGTCAATTGG CTCATCAGCT ACAGCAAGTA TATGCGTGCC 
TTGGATGAAT ACTATGACAA ACACTTCACA GAGTTCGTTC CTCTGAGGAC GAAAGCTAAG 
GAAATTCTGC AGGAAGAAGA AGACCTGGCA GAAATTGTAC AGCTTGTGGG AAAGGCTTCT 
TTGGCAGAAA CAGATAAAAT CACTCTGGAG GTAGCAAAAC TTATCAAAGA TGATTTCCTA 
CAACAAAATG GATATACTCC TTATGACAGG TTCTGCCCAT TCTACAAGAC AGTAGGGATG 
CTGTCCAACA TGATTGCATT TTATGATATG GCTCGTAGAG CTGTTGAAAC CACTGCCCAG 
AGTGACAATA AAATCACATG GTCCATTATT CGTGAGCACA TGGGAGACAT CCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCACTG AAAGATGGTG AGGCAAAGAT CAAAAGCGAC 
TATGCACAAC TTCTTGAAGA CATGCAGAAT GCATTCCGTA GCCTTGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001681.2
CDS	109..1962
Translation	MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	NM_001690.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001690.3

ORF Insert Sequence:

ATGGATTTTT CCAAGCTACC CAAAATACTC GATGAAGATA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGGG TCTCAGGACC TGTGGTTACA GCCTGTGACA TGGCGGGTGC AGCCATGTAT 
GAGCTGGTGA GAGTGGGCCA CAGCGAATTG GTTGGAGAGA TTATTCGATT GGAGGGTGAC 
ATGGCTACTA TTCAGGTGTA TGAAGAAACT TCTGGTGTGT CTGTTGGAGA TCCTGTACTT 
CGCACTGGTA AACCCCTCTC TGTAGAGCTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAAA GACCTTTGTC GGATATCAGC AGTCAGACCC AAAGCATCTA CATCCCCAGA 
GGAGTAAACG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG ACTTTACACC TTGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGCGGA GACATTTATG GAATTGTCAG TGAGAACTCG 
CTTATCAAAC ACAAAATCAT GTTACCCCCA CGAAACAGAG GAACTGTAAC TTACATTGCT 
CCACCTGGGA ATTATGATAC CTCTGATGTT GTCTTGGAGC TTGAATTTGA AGGTGTAAAG 
GAGAAGTTCA CCATGGTGCA AGTATGGCCT GTACGTCAAG TTCGACCTGT CACTGAGAAG 
CTGCCAGCCA ATCATCCTCT GTTGACTGGC CAGAGAGTCC TTGATGCCCT TTTTCCGTGT 
GTCCAGGGAG GAACTACTGC TATCCCTGGA GCCTTTGGCT GTGGAAAGAC AGTGATATCA 
CAGTCTCTAT CCAAGTATTC TAACAGTGAT GTAATCATCT ATGTAGGATG TGGTGAAAGA 
GGAAATGAGA TGTCTGAAGT CCTCCGGGAC TTCCCAGAGC TCACAATGGA GGTTGATGGT 
AAGGTAGAGT CAATTATGAA GAGGACAGCT TTGGTAGCCA ATACCTCCAA TATGCCTGTT 
GCTGCTAGAG AAGCCTCTAT TTATACTGGA ATCACACTGT CAGAGTACTT CCGTGACATG 
GGCTATCATG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTTAGAGAA 
ATCTCTGGTC GTTTAGCTGA AATGCCTGCA GATAGTGGAT ATCCAGCCTA TCTTGGTGCC 
CGTCTGGCCT CGTTTTATGA ACGAGCAGGC AGGGTGAAAT GTCTTGGAAA TCCTGAAAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTTACATCTG CCACTCTTGG TATCGTTCAG GTGTTCTGGG GCTTAGATAA GAAACTAGCT 
CAACGTAAGC ATTTCCCCTC TGTCAATTGG CTCATCAGCT ACAGCAAGTA TATGCGTGCC 
TTGGATGAAT ACTATGACAA ACACTTCACA GAGTTCGTTC CTCTGAGGAC GAAAGCTAAG 
GAAATTCTGC AGGAAGAAGA AGACCTGGCA GAAATTGTAC AGCTTGTGGG AAAGGCTTCT 
TTGGCAGAAA CAGATAAAAT CACTCTGGAG GTAGCAAAAC TTATCAAAGA TGATTTCCTA 
CAACAAAATG GATATACTCC TTATGACAGG TTCTGCCCAT TCTACAAGAC AGTAGGGATG 
CTGTCCAACA TGATTGCATT TTATGATATG GCTCGTAGAG CTGTTGAAAC CACTGCCCAG 
AGTGACAATA AAATCACATG GTCCATTATT CGTGAGCACA TGGGAGACAT CCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCACTG AAAGATGGTG AGGCAAAGAT CAAAAGCGAC 
TATGCACAAC TTCTTGAAGA CATGCAGAAT GCATTCCGTA GCCTTGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1A ( NM_001690.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001681.2 Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Price & Availability↑

CloneID	OHu55807
Clone ID Related Accession (Same CDS sequence)	NM_001690.3 , NM_001690.4
Accession Version	NM_001690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	V-type proton ATPase catalytic subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC350361.1, AK314779.1, AF339827.1 and BC012169.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001690.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.257939.1, SRR1660809.46685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000273398.8/ ENSP00000273398.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTTTT CCAAGCTACC CAAAATACTC GATGAAGATA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGGG TCTCAGGACC TGTGGTTACA GCCTGTGACA TGGCGGGTGC AGCCATGTAT 
GAGCTGGTGA GAGTGGGCCA CAGCGAATTG GTTGGAGAGA TTATTCGATT GGAGGGTGAC 
ATGGCTACTA TTCAGGTGTA TGAAGAAACT TCTGGTGTGT CTGTTGGAGA TCCTGTACTT 
CGCACTGGTA AACCCCTCTC TGTAGAGCTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAAA GACCTTTGTC GGATATCAGC AGTCAGACCC AAAGCATCTA CATCCCCAGA 
GGAGTAAACG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG ACTTTACACC TTGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGCGGA GACATTTATG GAATTGTCAG TGAGAACTCG 
CTTATCAAAC ACAAAATCAT GTTACCCCCA CGAAACAGAG GAACTGTAAC TTACATTGCT 
CCACCTGGGA ATTATGATAC CTCTGATGTT GTCTTGGAGC TTGAATTTGA AGGTGTAAAG 
GAGAAGTTCA CCATGGTGCA AGTATGGCCT GTACGTCAAG TTCGACCTGT CACTGAGAAG 
CTGCCAGCCA ATCATCCTCT GTTGACTGGC CAGAGAGTCC TTGATGCCCT TTTTCCGTGT 
GTCCAGGGAG GAACTACTGC TATCCCTGGA GCCTTTGGCT GTGGAAAGAC AGTGATATCA 
CAGTCTCTAT CCAAGTATTC TAACAGTGAT GTAATCATCT ATGTAGGATG TGGTGAAAGA 
GGAAATGAGA TGTCTGAAGT CCTCCGGGAC TTCCCAGAGC TCACAATGGA GGTTGATGGT 
AAGGTAGAGT CAATTATGAA GAGGACAGCT TTGGTAGCCA ATACCTCCAA TATGCCTGTT 
GCTGCTAGAG AAGCCTCTAT TTATACTGGA ATCACACTGT CAGAGTACTT CCGTGACATG 
GGCTATCATG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTTAGAGAA 
ATCTCTGGTC GTTTAGCTGA AATGCCTGCA GATAGTGGAT ATCCAGCCTA TCTTGGTGCC 
CGTCTGGCCT CGTTTTATGA ACGAGCAGGC AGGGTGAAAT GTCTTGGAAA TCCTGAAAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTTACATCTG CCACTCTTGG TATCGTTCAG GTGTTCTGGG GCTTAGATAA GAAACTAGCT 
CAACGTAAGC ATTTCCCCTC TGTCAATTGG CTCATCAGCT ACAGCAAGTA TATGCGTGCC 
TTGGATGAAT ACTATGACAA ACACTTCACA GAGTTCGTTC CTCTGAGGAC GAAAGCTAAG 
GAAATTCTGC AGGAAGAAGA AGACCTGGCA GAAATTGTAC AGCTTGTGGG AAAGGCTTCT 
TTGGCAGAAA CAGATAAAAT CACTCTGGAG GTAGCAAAAC TTATCAAAGA TGATTTCCTA 
CAACAAAATG GATATACTCC TTATGACAGG TTCTGCCCAT TCTACAAGAC AGTAGGGATG 
CTGTCCAACA TGATTGCATT TTATGATATG GCTCGTAGAG CTGTTGAAAC CACTGCCCAG 
AGTGACAATA AAATCACATG GTCCATTATT CGTGAGCACA TGGGAGACAT CCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCACTG AAAGATGGTG AGGCAAAGAT CAAAAGCGAC 
TATGCACAAC TTCTTGAAGA CATGCAGAAT GCATTCCGTA GCCTTGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001681.2
CDS	93..1946
Translation	MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	NM_001690.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001690.4

ORF Insert Sequence:

ATGGATTTTT CCAAGCTACC CAAAATACTC GATGAAGATA AAGAAAGCAC ATTTGGTTAT 
GTGCATGGGG TCTCAGGACC TGTGGTTACA GCCTGTGACA TGGCGGGTGC AGCCATGTAT 
GAGCTGGTGA GAGTGGGCCA CAGCGAATTG GTTGGAGAGA TTATTCGATT GGAGGGTGAC 
ATGGCTACTA TTCAGGTGTA TGAAGAAACT TCTGGTGTGT CTGTTGGAGA TCCTGTACTT 
CGCACTGGTA AACCCCTCTC TGTAGAGCTT GGTCCTGGCA TTATGGGAGC CATTTTTGAT 
GGTATTCAAA GACCTTTGTC GGATATCAGC AGTCAGACCC AAAGCATCTA CATCCCCAGA 
GGAGTAAACG TGTCTGCTCT TAGCAGAGAT ATCAAATGGG ACTTTACACC TTGCAAAAAC 
CTACGGGTTG GTAGTCATAT CACTGGCGGA GACATTTATG GAATTGTCAG TGAGAACTCG 
CTTATCAAAC ACAAAATCAT GTTACCCCCA CGAAACAGAG GAACTGTAAC TTACATTGCT 
CCACCTGGGA ATTATGATAC CTCTGATGTT GTCTTGGAGC TTGAATTTGA AGGTGTAAAG 
GAGAAGTTCA CCATGGTGCA AGTATGGCCT GTACGTCAAG TTCGACCTGT CACTGAGAAG 
CTGCCAGCCA ATCATCCTCT GTTGACTGGC CAGAGAGTCC TTGATGCCCT TTTTCCGTGT 
GTCCAGGGAG GAACTACTGC TATCCCTGGA GCCTTTGGCT GTGGAAAGAC AGTGATATCA 
CAGTCTCTAT CCAAGTATTC TAACAGTGAT GTAATCATCT ATGTAGGATG TGGTGAAAGA 
GGAAATGAGA TGTCTGAAGT CCTCCGGGAC TTCCCAGAGC TCACAATGGA GGTTGATGGT 
AAGGTAGAGT CAATTATGAA GAGGACAGCT TTGGTAGCCA ATACCTCCAA TATGCCTGTT 
GCTGCTAGAG AAGCCTCTAT TTATACTGGA ATCACACTGT CAGAGTACTT CCGTGACATG 
GGCTATCATG TCAGTATGAT GGCTGACTCT ACCTCTAGAT GGGCTGAGGC CCTTAGAGAA 
ATCTCTGGTC GTTTAGCTGA AATGCCTGCA GATAGTGGAT ATCCAGCCTA TCTTGGTGCC 
CGTCTGGCCT CGTTTTATGA ACGAGCAGGC AGGGTGAAAT GTCTTGGAAA TCCTGAAAGA 
GAAGGGAGTG TCAGCATTGT AGGAGCAGTT TCTCCACCTG GTGGTGATTT TTCTGATCCA 
GTTACATCTG CCACTCTTGG TATCGTTCAG GTGTTCTGGG GCTTAGATAA GAAACTAGCT 
CAACGTAAGC ATTTCCCCTC TGTCAATTGG CTCATCAGCT ACAGCAAGTA TATGCGTGCC 
TTGGATGAAT ACTATGACAA ACACTTCACA GAGTTCGTTC CTCTGAGGAC GAAAGCTAAG 
GAAATTCTGC AGGAAGAAGA AGACCTGGCA GAAATTGTAC AGCTTGTGGG AAAGGCTTCT 
TTGGCAGAAA CAGATAAAAT CACTCTGGAG GTAGCAAAAC TTATCAAAGA TGATTTCCTA 
CAACAAAATG GATATACTCC TTATGACAGG TTCTGCCCAT TCTACAAGAC AGTAGGGATG 
CTGTCCAACA TGATTGCATT TTATGATATG GCTCGTAGAG CTGTTGAAAC CACTGCCCAG 
AGTGACAATA AAATCACATG GTCCATTATT CGTGAGCACA TGGGAGACAT CCTCTATAAA 
CTTTCCTCCA TGAAATTCAA GGATCCACTG AAAGATGGTG AGGCAAAGAT CAAAAGCGAC 
TATGCACAAC TTCTTGAAGA CATGCAGAAT GCATTCCGTA GCCTTGAAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and Transcriptional Regulation of Human ATP6V1A Gene in Gastric Cancers.
Scientific reports7(1)3015(2017 Jun)
Wang P,Wang L,Sha J,Lou G,Lu N,Hang B,Mao JH,Zou X

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
eLife6(2017 03)
Miles AL,Burr SP,Grice GL,Nathan JA

Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.
American journal of human genetics100(2)216-227(2017 Feb)
Van Damme T,Gardeitchik T,Mohamed M,Guerrero-Castillo S,Freisinger P,Guillemyn B,Kariminejad A,Dalloyaux D,van Kraaij S,Lefeber DJ,Syx D,Steyaert W,De Rycke R,Hoischen A,Kamsteeg EJ,Wong SY,van Scherpenzeel M,Jamali P,Brandt U,Nijtmans L,Korenke GC,Chung BHY,Mak CCY,Hausser I,Kornak U,Fischer-Zirnsak B,Strom TM,Meitinger T,Alanay Y,Utine GE,Leung PKC,Ghaderi-Sohi S,Coucke P,Symoens S,De Paepe A,Thiel C,Haack TB,Malfait F,Morava E,Callewaert B,Wevers RA

Expression and role of V1A subunit of V-ATPases in gastric cancer cells.
International journal of clinical oncology20(4)725-35(2015 Aug)
Liu P,Chen H,Han L,Zou X,Shen W

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ATP6V1A cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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