TSC2 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc70003 XM_016928620.1
Latest version!
Pan troglodytes tuberous sclerosis 2 (TSC2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc99265 XM_024350033.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99266 XM_024350034.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99267 XM_024350035.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99268 XM_024350036.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99269 XM_024350037.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99270 XM_024350038.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99271 XM_024350039.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99272 XM_024350040.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc99273 XM_024350041.1
Latest version!
Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc70003
    Clone ID Related Accession (Same CDS sequence) XM_016928620.1
    Accession Version XM_016928620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product tuberin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006483.4) and transcript sequence (GABC01019420.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 376 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCTAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016784109.1
    CDS118..5472
    Translation

    Target ORF information:

    RefSeq Version XM_016928620.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tuberous sclerosis 2 (TSC2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016928620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCTAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99265
    Clone ID Related Accession (Same CDS sequence) XM_024350033.1
    Accession Version XM_024350033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG
    GAGGAGGGAA TTCCGGGCGA GGTTCCTGTG CCGGTGGCGC CCCCAGGGTT GGAGGACGTC
    GAGGCAGCGC TAGGCATGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAGTCTCC
    AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG
    CGAGCCGTCT CCTCAGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG
    CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTTGGGGAC
    CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA
    GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG
    CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT
    TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGATGCCTTA
    AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCGG GCATCAACCC CAGTTTCGTG
    TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG
    CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC
    GACACCCACA AGATCGCCGT CCTCTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC
    ATCCTGTCCA ACGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG
    CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT
    GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC
    ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC
    CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCTG GTGAGGACTT CAAGCTCGGC
    ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT
    ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTACCC CTCCAAGTGG
    ATTGCCCGGC TCCGCCACAT CAAGAGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC
    TCCAACCCCA GCCTGCCTCT GGTGCACCCT CCGTCCCATA CCAAAGCCCC TGCACAGACT
    CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG
    GAGGACTTCA CCGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205801.1
    CDS194..5617
    Translation

    Target ORF information:

    RefSeq Version XM_024350033.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG
    GAGGAGGGAA TTCCGGGCGA GGTTCCTGTG CCGGTGGCGC CCCCAGGGTT GGAGGACGTC
    GAGGCAGCGC TAGGCATGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAGTCTCC
    AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG
    CGAGCCGTCT CCTCAGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG
    CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTTGGGGAC
    CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA
    GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG
    CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT
    TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGATGCCTTA
    AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCGG GCATCAACCC CAGTTTCGTG
    TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG
    CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC
    GACACCCACA AGATCGCCGT CCTCTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC
    ATCCTGTCCA ACGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG
    CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT
    GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC
    ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC
    CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCTG GTGAGGACTT CAAGCTCGGC
    ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT
    ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTACCC CTCCAAGTGG
    ATTGCCCGGC TCCGCCACAT CAAGAGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC
    TCCAACCCCA GCCTGCCTCT GGTGCACCCT CCGTCCCATA CCAAAGCCCC TGCACAGACT
    CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG
    GAGGACTTCA CCGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99266
    Clone ID Related Accession (Same CDS sequence) XM_024350034.1
    Accession Version XM_024350034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG
    TCTGCAGATG AGAACTCGGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG
    CTGACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTG
    CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA
    GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGTGTGCAT
    GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGCGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC
    CTGGACGTGC CGGCTTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA
    CCAGCCGTGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTATGCA GGAGAAGACG
    AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG
    CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG
    GACATCAACA ACATGCCCCT GCAGGAGCTG TCCAACGCCC TCATGGCGGC TGAGCGCTTC
    AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC
    AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCCGCCGT GGTCATGGAG
    GAGGGAATTC CGGGCGAGGT TCCTGTGCCG GTGGCGCCCC CAGGGTTGGA GGACGTCGAG
    GCAGCGCTAG GCATGGACAG GCGCACAGAC GCCTACAGCA GGTCGTCCTC AGTCTCCAGC
    CAGGAGGAGA AGTCGCTCCA CGCGGAGGAG CTGGTTGGCA GGGGCATCCC CATCGAGCGA
    GCCGTCTCCT CAGAGGGTGG CCGGCCCTCT GTGGACCTCT CCTTCCAGCC CTCGCAGCCC
    CTGAGCAAGT CCAGCTCCTC TCCCGAGCTG CAGACTCTGC AGGACATCCT TGGGGACCCT
    GGGGACAAGG CCGACGTGGG CCGGCTGAGC CCTGAGGTTA AGGCCCGGTC ACAGTCAGGG
    ACCCTGGACG GGGAAAGTGC TGCCTGGTCG GCCTCGGGCG AAGACAGTCG GGGCCAGCCC
    GAGGGTCCCT TGCCTTCCAG CTCCCCCCGC TCGCCCAGTG GCCTCCGGCC CCGAGGTTAC
    ACCATCTCCG ACTCGGCCCC ATCACGCAGG GGCAAGAGAG TAGAGAGGGA TGCCTTAAAG
    AGCAGAGCCA CAGCCTCCAA TGCAGAGAAA GTGCCGGGCA TCAACCCCAG TTTCGTGTTC
    CTGCAGCTCT ACCATTCCCC CTTCTTTGGC GACGAGTCAA ACAAGCCAAT CCTGCTGCCC
    AATGAGTCAC AGTCCTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC ATCATACGAC
    ACCCACAAGA TCGCCGTCCT CTATGTTGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC
    CTGTCCAACG AGCATGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGCCT GGGCCGGCTC
    ATCGAGCTGA AGGACTGCCA GCCGGACAAG GTGTACCTGG GAGGCCTGGA CGTGTGTGGT
    GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCCGT CTTCCACATC
    GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GCGACAAGAA GCGCCACCTG
    GGCAACGACT TTGTGTCCAT TGTCTACAAT GACTCTGGTG AGGACTTCAA GCTCGGCACC
    ATCAAGGGCC AGTTCAACTT TGTCCACGTG ATCGTCACCC CGCTGGACTA CGAGTGCAAC
    CTGGTGTCCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAG
    ATCGTGTCTG ACCGCAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCAAATATG
    GCCTCACAGG TGCATCATAG CCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATT
    GCCCGGCTCC GCCACATCAA GAGGCTCCGC CAGCGGATCT GCGAGGAAGC CGCCTACTCC
    AACCCCAGCC TGCCTCTGGT GCACCCTCCG TCCCATACCA AAGCCCCTGC ACAGACTCCA
    GCCGAGCCCA CACCTGGCTA TGAGGTGGGC CAGCGGAAGC GCCTCATCTC CTCGGTGGAG
    GACTTCACCG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205802.1
    CDS137..5557
    Translation

    Target ORF information:

    RefSeq Version XM_024350034.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG
    TCTGCAGATG AGAACTCGGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG
    CTGACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTG
    CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA
    GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGTGTGCAT
    GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGCGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC
    CTGGACGTGC CGGCTTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA
    CCAGCCGTGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTATGCA GGAGAAGACG
    AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG
    CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG
    GACATCAACA ACATGCCCCT GCAGGAGCTG TCCAACGCCC TCATGGCGGC TGAGCGCTTC
    AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC
    AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCCGCCGT GGTCATGGAG
    GAGGGAATTC CGGGCGAGGT TCCTGTGCCG GTGGCGCCCC CAGGGTTGGA GGACGTCGAG
    GCAGCGCTAG GCATGGACAG GCGCACAGAC GCCTACAGCA GGTCGTCCTC AGTCTCCAGC
    CAGGAGGAGA AGTCGCTCCA CGCGGAGGAG CTGGTTGGCA GGGGCATCCC CATCGAGCGA
    GCCGTCTCCT CAGAGGGTGG CCGGCCCTCT GTGGACCTCT CCTTCCAGCC CTCGCAGCCC
    CTGAGCAAGT CCAGCTCCTC TCCCGAGCTG CAGACTCTGC AGGACATCCT TGGGGACCCT
    GGGGACAAGG CCGACGTGGG CCGGCTGAGC CCTGAGGTTA AGGCCCGGTC ACAGTCAGGG
    ACCCTGGACG GGGAAAGTGC TGCCTGGTCG GCCTCGGGCG AAGACAGTCG GGGCCAGCCC
    GAGGGTCCCT TGCCTTCCAG CTCCCCCCGC TCGCCCAGTG GCCTCCGGCC CCGAGGTTAC
    ACCATCTCCG ACTCGGCCCC ATCACGCAGG GGCAAGAGAG TAGAGAGGGA TGCCTTAAAG
    AGCAGAGCCA CAGCCTCCAA TGCAGAGAAA GTGCCGGGCA TCAACCCCAG TTTCGTGTTC
    CTGCAGCTCT ACCATTCCCC CTTCTTTGGC GACGAGTCAA ACAAGCCAAT CCTGCTGCCC
    AATGAGTCAC AGTCCTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC ATCATACGAC
    ACCCACAAGA TCGCCGTCCT CTATGTTGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC
    CTGTCCAACG AGCATGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGCCT GGGCCGGCTC
    ATCGAGCTGA AGGACTGCCA GCCGGACAAG GTGTACCTGG GAGGCCTGGA CGTGTGTGGT
    GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCCGT CTTCCACATC
    GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GCGACAAGAA GCGCCACCTG
    GGCAACGACT TTGTGTCCAT TGTCTACAAT GACTCTGGTG AGGACTTCAA GCTCGGCACC
    ATCAAGGGCC AGTTCAACTT TGTCCACGTG ATCGTCACCC CGCTGGACTA CGAGTGCAAC
    CTGGTGTCCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAG
    ATCGTGTCTG ACCGCAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCAAATATG
    GCCTCACAGG TGCATCATAG CCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATT
    GCCCGGCTCC GCCACATCAA GAGGCTCCGC CAGCGGATCT GCGAGGAAGC CGCCTACTCC
    AACCCCAGCC TGCCTCTGGT GCACCCTCCG TCCCATACCA AAGCCCCTGC ACAGACTCCA
    GCCGAGCCCA CACCTGGCTA TGAGGTGGGC CAGCGGAAGC GCCTCATCTC CTCGGTGGAG
    GACTTCACCG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99267
    Clone ID Related Accession (Same CDS sequence) XM_024350035.1
    Accession Version XM_024350035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205803.1
    CDS194..5548
    Translation

    Target ORF information:

    RefSeq Version XM_024350035.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99268
    Clone ID Related Accession (Same CDS sequence) XM_024350036.1
    Accession Version XM_024350036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG
    GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC
    GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG
    GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT
    CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TGGACGAGTA CATCGCAAGG
    ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC
    TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG
    CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG
    AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC
    CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCTGTGTTT
    TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA
    TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG
    ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG
    TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC
    CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG
    AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG
    AGGCCTGAGT CCTCCCTTCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC
    AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC
    CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG
    CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC
    GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG
    GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC
    TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG
    GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC
    CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC
    TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC
    CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC
    GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG
    AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC
    GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG
    AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG
    CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC
    TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC
    TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT
    AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC
    CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC
    ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC GGCCACAGCC
    AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC
    AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC
    CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC
    ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTCGTCCCTT TCATCACTAA GGGCCTGCGG
    TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT
    ACTAGTCTCA ACGAGAGACC CAAGAGTCTG AGGATAGCCA GACCCCCCAA ACAAGGCTTG
    AATAACTCTC CACCCGTGAA AGAATTCAAG GAGAGCTCTG CAGCCGAGGC CTTCCGGTGC
    CGCAGCATCA GTGTGTCTGA ACATGTGGTC CGCAGCAGGA TACAGACGTC CCTCACCAGT
    GCCAGCTTGG GGTCTGCAGA TGAGAACTCG GTGGCCCAGG CTGACGATAG CCTGAAAAAC
    CTCCACCTGG AGCTGACGGA AACCTGTCTG GACATGATGG CTCGATACGT CTTCTCCAAC
    TTCACGGCTG TGCCGAAGAG GTCTCCTGTG GGCGAGTTCC TCCTAGCGGG TGGCAGGACC
    AAAACCTGGC TGGTTGGGAA CAAGCTTGTC ACTGTGACGA CAAGCGTGGG AACCGGGACC
    CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC
    CCCGGTGTGC ATGTGAGACA GACCAAGGAG GCGCCGGCCA AGCTGGAGTC CCAGGCTGGG
    CAGCAGGTGT CCCGTGGGGC CCGGGACCGC GTCCGTTCCA TGTCGGGGGG CCATGGTCTT
    CGAGTTGGCG CCCTGGACGT GCCGGCTTCC CAGTTCCTGG GCAGTGCCAC TTCTCCAGGA
    CCACGGACTG CACCAGCCGT GAAACCTGAG AAGGCCTCAG CTGGCACCCG GGTTCCTATG
    CAGGAGAAGA CGAACCTGGC GGCCTATGTG CCCCTGCTGA CCCAGGGCTG GGCGGAGATC
    CTGGTCCGGA GGCCCACAGG GAACACCAGC TGGCTGATGA GCCTGGAGAA CCCGCTCAGC
    CCTTTCTCCT CGGACATCAA CAACATGCCC CTGCAGGAGC TGTCCAACGC CCTCATGGCG
    GCTGAGCGCT TCAAGGAGCA CCGGGACACA GCCCTGTACA AGTCACTGTC GGTGCCGGCA
    GCCAGCACGG CCAAACCCCC TCCTCTGCCT CGCTCCAACA CAGTGGCCTC TTTCTCCTCC
    CTGTACCAGT CCAGCTGCCA AGGACAGCTG CACAGGAGCG TTTCCTGGGC AGACTCCGCC
    GTGGTCATGG AGGAGGGAAT TCCGGGCGAG GTTCCTGTGC CGGTGGCGCC CCCAGGGTTG
    GAGGACGTCG AGGCAGCGCT AGGCATGGAC AGGCGCACAG ACGCCTACAG CAGGTCGTCC
    TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC CACGCGGAGG AGCTGGTTGG CAGGGGCATC
    CCCATCGAGC GAGCCGTCTC CTCAGAGGGT GGCCGGCCCT CTGTGGACCT CTCCTTCCAG
    CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC TCTCCCGAGC TGCAGACTCT GCAGGACATC
    CTTGGGGACC CTGGGGACAA GGCCGACGTG GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG
    TCACAGTCAG GGACCCTGGA CGGGGAAAGT GCTGCCTGGT CGGCCTCGGG CGAAGACAGT
    CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG
    CCCCGAGGTT ACACCATCTC CGACTCGGCC CCATCACGCA GGGGCAAGAG AGTAGAGAGG
    GATGCCTTAA AGAGCAGAGC CACAGCCTCC AATGCAGAGA AAGTGCCGGG CATCAACCCC
    AGTTTCGTGT TCCTGCAGCT CTACCATTCC CCCTTCTTTG GCGACGAGTC AAACAAGCCA
    ATCCTGCTGC CCAATGAGTC ACAGTCCTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC
    CCATCATACG ACACCCACAA GATCGCCGTC CTCTATGTTG GAGAAGGCCA GAGCAACAGC
    GAGCTCGCCA TCCTGTCCAA CGAGCATGGC TCCTACAGGT ACACGGAGTT CCTGACGGGC
    CTGGGCCGGC TCATCGAGCT GAAGGACTGC CAGCCGGACA AGGTGTACCT GGGAGGCCTG
    GACGTGTGTG GTGAGGACGG CCAGTTCACC TACTGCTGGC ACGATGACAT CATGCAAGCC
    GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGCGACAAG
    AAGCGCCACC TGGGCAACGA CTTTGTGTCC ATTGTCTACA ATGACTCTGG TGAGGACTTC
    AAGCTCGGCA CCATCAAGGG CCAGTTCAAC TTTGTCCACG TGATCGTCAC CCCGCTGGAC
    TACGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG
    CACGCAAATA TGGCCTCACA GGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTACCCC
    TCCAAGTGGA TTGCCCGGCT CCGCCACATC AAGAGGCTCC GCCAGCGGAT CTGCGAGGAA
    GCCGCCTACT CCAACCCCAG CCTGCCTCTG GTGCACCCTC CGTCCCATAC CAAAGCCCCT
    GCACAGACTC CAGCCGAGCC CACACCTGGC TATGAGGTGG GCCAGCGGAA GCGCCTCATC
    TCCTCGGTGG AGGACTTCAC CGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205804.1
    CDS137..5449
    Translation

    Target ORF information:

    RefSeq Version XM_024350036.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG
    GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC
    GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG
    GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT
    CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TGGACGAGTA CATCGCAAGG
    ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC
    TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG
    CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG
    AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC
    CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCTGTGTTT
    TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA
    TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG
    ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG
    TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC
    CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG
    AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG
    AGGCCTGAGT CCTCCCTTCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC
    AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC
    CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG
    CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC
    GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG
    GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC
    TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG
    GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC
    CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC
    TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC
    CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC
    GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG
    AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC
    GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG
    AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG
    CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC
    TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC
    TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT
    AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC
    CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC
    ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC GGCCACAGCC
    AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC
    AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC
    CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC
    ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTCGTCCCTT TCATCACTAA GGGCCTGCGG
    TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT
    ACTAGTCTCA ACGAGAGACC CAAGAGTCTG AGGATAGCCA GACCCCCCAA ACAAGGCTTG
    AATAACTCTC CACCCGTGAA AGAATTCAAG GAGAGCTCTG CAGCCGAGGC CTTCCGGTGC
    CGCAGCATCA GTGTGTCTGA ACATGTGGTC CGCAGCAGGA TACAGACGTC CCTCACCAGT
    GCCAGCTTGG GGTCTGCAGA TGAGAACTCG GTGGCCCAGG CTGACGATAG CCTGAAAAAC
    CTCCACCTGG AGCTGACGGA AACCTGTCTG GACATGATGG CTCGATACGT CTTCTCCAAC
    TTCACGGCTG TGCCGAAGAG GTCTCCTGTG GGCGAGTTCC TCCTAGCGGG TGGCAGGACC
    AAAACCTGGC TGGTTGGGAA CAAGCTTGTC ACTGTGACGA CAAGCGTGGG AACCGGGACC
    CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC
    CCCGGTGTGC ATGTGAGACA GACCAAGGAG GCGCCGGCCA AGCTGGAGTC CCAGGCTGGG
    CAGCAGGTGT CCCGTGGGGC CCGGGACCGC GTCCGTTCCA TGTCGGGGGG CCATGGTCTT
    CGAGTTGGCG CCCTGGACGT GCCGGCTTCC CAGTTCCTGG GCAGTGCCAC TTCTCCAGGA
    CCACGGACTG CACCAGCCGT GAAACCTGAG AAGGCCTCAG CTGGCACCCG GGTTCCTATG
    CAGGAGAAGA CGAACCTGGC GGCCTATGTG CCCCTGCTGA CCCAGGGCTG GGCGGAGATC
    CTGGTCCGGA GGCCCACAGG GAACACCAGC TGGCTGATGA GCCTGGAGAA CCCGCTCAGC
    CCTTTCTCCT CGGACATCAA CAACATGCCC CTGCAGGAGC TGTCCAACGC CCTCATGGCG
    GCTGAGCGCT TCAAGGAGCA CCGGGACACA GCCCTGTACA AGTCACTGTC GGTGCCGGCA
    GCCAGCACGG CCAAACCCCC TCCTCTGCCT CGCTCCAACA CAGTGGCCTC TTTCTCCTCC
    CTGTACCAGT CCAGCTGCCA AGGACAGCTG CACAGGAGCG TTTCCTGGGC AGACTCCGCC
    GTGGTCATGG AGGAGGGAAT TCCGGGCGAG GTTCCTGTGC CGGTGGCGCC CCCAGGGTTG
    GAGGACGTCG AGGCAGCGCT AGGCATGGAC AGGCGCACAG ACGCCTACAG CAGGTCGTCC
    TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC CACGCGGAGG AGCTGGTTGG CAGGGGCATC
    CCCATCGAGC GAGCCGTCTC CTCAGAGGGT GGCCGGCCCT CTGTGGACCT CTCCTTCCAG
    CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC TCTCCCGAGC TGCAGACTCT GCAGGACATC
    CTTGGGGACC CTGGGGACAA GGCCGACGTG GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG
    TCACAGTCAG GGACCCTGGA CGGGGAAAGT GCTGCCTGGT CGGCCTCGGG CGAAGACAGT
    CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG
    CCCCGAGGTT ACACCATCTC CGACTCGGCC CCATCACGCA GGGGCAAGAG AGTAGAGAGG
    GATGCCTTAA AGAGCAGAGC CACAGCCTCC AATGCAGAGA AAGTGCCGGG CATCAACCCC
    AGTTTCGTGT TCCTGCAGCT CTACCATTCC CCCTTCTTTG GCGACGAGTC AAACAAGCCA
    ATCCTGCTGC CCAATGAGTC ACAGTCCTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC
    CCATCATACG ACACCCACAA GATCGCCGTC CTCTATGTTG GAGAAGGCCA GAGCAACAGC
    GAGCTCGCCA TCCTGTCCAA CGAGCATGGC TCCTACAGGT ACACGGAGTT CCTGACGGGC
    CTGGGCCGGC TCATCGAGCT GAAGGACTGC CAGCCGGACA AGGTGTACCT GGGAGGCCTG
    GACGTGTGTG GTGAGGACGG CCAGTTCACC TACTGCTGGC ACGATGACAT CATGCAAGCC
    GTCTTCCACA TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGCGACAAG
    AAGCGCCACC TGGGCAACGA CTTTGTGTCC ATTGTCTACA ATGACTCTGG TGAGGACTTC
    AAGCTCGGCA CCATCAAGGG CCAGTTCAAC TTTGTCCACG TGATCGTCAC CCCGCTGGAC
    TACGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG
    CACGCAAATA TGGCCTCACA GGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTACCCC
    TCCAAGTGGA TTGCCCGGCT CCGCCACATC AAGAGGCTCC GCCAGCGGAT CTGCGAGGAA
    GCCGCCTACT CCAACCCCAG CCTGCCTCTG GTGCACCCTC CGTCCCATAC CAAAGCCCCT
    GCACAGACTC CAGCCGAGCC CACACCTGGC TATGAGGTGG GCCAGCGGAA GCGCCTCATC
    TCCTCGGTGG AGGACTTCAC CGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99269
    Clone ID Related Accession (Same CDS sequence) XM_024350037.1
    Accession Version XM_024350037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA
    GATGAGAACT CGGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTGACG
    GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTGCCGAAG
    AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG
    GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGTGT GCATGTGAGA
    CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GCGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC
    GTGCCGGCTT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC
    GTGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTA TGCAGGAGAA GACGAACCTG
    GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC
    AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG
    CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC
    CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC
    CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205805.1
    CDS137..5431
    Translation

    Target ORF information:

    RefSeq Version XM_024350037.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA
    GATGAGAACT CGGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTGACG
    GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTGCCGAAG
    AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG
    GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGTGT GCATGTGAGA
    CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GCGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC
    GTGCCGGCTT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC
    GTGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTA TGCAGGAGAA GACGAACCTG
    GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC
    AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG
    CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC
    CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC
    CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99270
    Clone ID Related Accession (Same CDS sequence) XM_024350038.1
    Accession Version XM_024350038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT
    GAGAACTCGG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTGACGGAA
    ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT GCCGAAGAGG
    TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC
    TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGTGTGCA TGTGAGACAG
    ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGCG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG
    CCGGCTTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGTG
    AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTATGC AGGAGAAGAC GAACCTGGCG
    GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC
    AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC
    CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT
    CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA
    GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAATT
    CCGGGCGAGG TTCCTGTGCC GGTGGCGCCC CCAGGGTTGG AGGACGTCGA GGCAGCGCTA
    GGCATGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG
    AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGCCGTCTCC
    TCAGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG
    TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TTGGGGACCC TGGGGACAAG
    GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC
    GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC
    TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC
    GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ATGCCTTAAA GAGCAGAGCC
    ACAGCCTCCA ATGCAGAGAA AGTGCCGGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC
    TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA
    CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG
    ATCGCCGTCC TCTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAC
    GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG
    AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC
    CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG
    ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC
    TTTGTGTCCA TTGTCTACAA TGACTCTGGT GAGGACTTCA AGCTCGGCAC CATCAAGGGC
    CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC
    CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT
    GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG
    GTGCATCATA GCCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT TGCCCGGCTC
    CGCCACATCA AGAGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC
    CTGCCTCTGG TGCACCCTCC GTCCCATACC AAAGCCCCTG CACAGACTCC AGCCGAGCCC
    ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC
    GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205806.1
    CDS137..5428
    Translation

    Target ORF information:

    RefSeq Version XM_024350038.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT
    GAGAACTCGG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTGACGGAA
    ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT GCCGAAGAGG
    TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC
    TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGTGTGCA TGTGAGACAG
    ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGCG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG
    CCGGCTTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGTG
    AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTATGC AGGAGAAGAC GAACCTGGCG
    GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC
    AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC
    CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT
    CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA
    GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAATT
    CCGGGCGAGG TTCCTGTGCC GGTGGCGCCC CCAGGGTTGG AGGACGTCGA GGCAGCGCTA
    GGCATGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG
    AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGCCGTCTCC
    TCAGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG
    TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TTGGGGACCC TGGGGACAAG
    GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC
    GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC
    TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC
    GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ATGCCTTAAA GAGCAGAGCC
    ACAGCCTCCA ATGCAGAGAA AGTGCCGGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC
    TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA
    CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG
    ATCGCCGTCC TCTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAC
    GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG
    AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC
    CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG
    ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC
    TTTGTGTCCA TTGTCTACAA TGACTCTGGT GAGGACTTCA AGCTCGGCAC CATCAAGGGC
    CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC
    CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT
    GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG
    GTGCATCATA GCCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT TGCCCGGCTC
    CGCCACATCA AGAGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC
    CTGCCTCTGG TGCACCCTCC GTCCCATACC AAAGCCCCTG CACAGACTCC AGCCGAGCCC
    ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC
    GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99271
    Clone ID Related Accession (Same CDS sequence) XM_024350039.1
    Accession Version XM_024350039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA
    GATGAGAACT CGGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTGACG
    GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTGCCGAAG
    AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG
    GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGTGT GCATGTGAGA
    CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GCGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC
    GTGCCGGCTT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC
    GTGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTA TGCAGGAGAA GACGAACCTG
    GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC
    AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG
    CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC
    CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AATTCCGGGC
    GAGGTTCCTG TGCCGGTGGC GCCCCCAGGG TTGGAGGACG TCGAGGCAGC GCTAGGCATG
    GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG
    CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGCCGT CTCCTCAGAG
    GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC
    TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTTGGGG ACCCTGGGGA CAAGGCCGAC
    GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA
    AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT
    TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG
    GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGATGCCT TAAAGAGCAG AGCCACAGCC
    TCCAATGCAG AGAAAGTGCC GGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT
    TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC
    TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC
    GTCCTCTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT
    GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC
    TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC
    ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC
    ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG
    TCCATTGTCT ACAATGACTC TGGTGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC
    AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG
    TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC
    AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT
    CATAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC
    ATCAAGAGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT
    CTGGTGCACC CTCCGTCCCA TACCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT
    GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205807.1
    CDS137..5362
    Translation

    Target ORF information:

    RefSeq Version XM_024350039.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA
    GATGAGAACT CGGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTGACG
    GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTGCCGAAG
    AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG
    GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGTGT GCATGTGAGA
    CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GCGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC
    GTGCCGGCTT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC
    GTGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTA TGCAGGAGAA GACGAACCTG
    GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC
    AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG
    CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC
    CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AATTCCGGGC
    GAGGTTCCTG TGCCGGTGGC GCCCCCAGGG TTGGAGGACG TCGAGGCAGC GCTAGGCATG
    GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG
    CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGCCGT CTCCTCAGAG
    GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC
    TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTTGGGG ACCCTGGGGA CAAGGCCGAC
    GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA
    AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT
    TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG
    GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGATGCCT TAAAGAGCAG AGCCACAGCC
    TCCAATGCAG AGAAAGTGCC GGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT
    TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC
    TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC
    GTCCTCTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT
    GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC
    TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC
    ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC
    ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG
    TCCATTGTCT ACAATGACTC TGGTGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC
    AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG
    TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC
    AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT
    CATAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC
    ATCAAGAGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT
    CTGGTGCACC CTCCGTCCCA TACCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT
    GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99272
    Clone ID Related Accession (Same CDS sequence) XM_024350040.1
    Accession Version XM_024350040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG
    GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC
    GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG
    GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT
    CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TGGACGAGTA CATCGCAAGG
    ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC
    TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG
    CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG
    AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC
    CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCTGTGTTT
    TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA
    TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG
    ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG
    TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC
    CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG
    AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG
    AGGCCTGAGT CCTCCCTTCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC
    AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC
    CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG
    CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC
    GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG
    GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC
    TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG
    GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC
    CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC
    TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC
    CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC
    GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG
    AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC
    GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG
    AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG
    CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC
    TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC
    TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT
    AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC
    CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC
    ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC GGCCACAGCC
    AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC
    AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC
    CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC
    ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTCGTCCCTT TCATCACTAA GGGCCTGCGG
    TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT
    ACTAGTCTCA ACGAGAGACC CAAGAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205808.1
    CDS194..5308
    Translation

    Target ORF information:

    RefSeq Version XM_024350040.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG
    GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC
    GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG
    GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT
    CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TGGACGAGTA CATCGCAAGG
    ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC
    TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG
    CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG
    AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC
    CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCTGTGTTT
    TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA
    TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG
    ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG
    TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC
    CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG
    AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG
    AGGCCTGAGT CCTCCCTTCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC
    AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC
    CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG
    CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC
    GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG
    GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC
    TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG
    GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC
    CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC
    TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC
    CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC
    GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG
    AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC
    GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG
    AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG
    CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC
    TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC
    TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT
    AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC
    CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC
    ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC GGCCACAGCC
    AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC
    AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC
    CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC
    ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTCGTCCCTT TCATCACTAA GGGCCTGCGG
    TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT
    ACTAGTCTCA ACGAGAGACC CAAGAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG
    GGGTCTGCAG ATGAGAACTC GGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG
    GAGCTGACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT
    GTGCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA
    CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGTGTG
    CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG CGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC
    GCCCTGGACG TGCCGGCTTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT
    GCACCAGCCG TGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTAT GCAGGAGAAG
    ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG
    AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC
    TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG
    GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA
    ATTCCGGGCG AGGTTCCTGT GCCGGTGGCG CCCCCAGGGT TGGAGGACGT CGAGGCAGCG
    CTAGGCATGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG
    GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGCCGTC
    TCCTCAGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC
    AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTTGGGGA CCCTGGGGAC
    AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG
    GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT
    CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC
    TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGATGCCTT AAAGAGCAGA
    GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG
    CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG
    TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC
    AAGATCGCCG TCCTCTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC
    AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG
    CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC
    GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC
    CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC
    GACTTTGTGT CCATTGTCTA CAATGACTCT GGTGAGGACT TCAAGCTCGG CACCATCAAG
    GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA
    CAGGTGCATC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG
    CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC
    AGCCTGCCTC TGGTGCACCC TCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG
    CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC
    ACCGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99273
    Clone ID Related Accession (Same CDS sequence) XM_024350041.1
    Accession Version XM_024350041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product tuberin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036895.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT
    GAGAACTCGG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTGACGGAA
    ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT GCCGAAGAGG
    TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC
    TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGTGTGCA TGTGAGACAG
    ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGCG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG
    CCGGCTTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGTG
    AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTATGC AGGAGAAGAC GAACCTGGCG
    GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC
    AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC
    CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT
    CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAT TCCGGGCGAG
    GTTCCTGTGC CGGTGGCGCC CCCAGGGTTG GAGGACGTCG AGGCAGCGCT AGGCATGGAC
    AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC
    CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGCCGTCTC CTCAGAGGGT
    GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC
    TCTCCCGAGC TGCAGACTCT GCAGGACATC CTTGGGGACC CTGGGGACAA GGCCGACGTG
    GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT
    GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC
    AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC
    CCATCACGCA GGGGCAAGAG AGTAGAGAGG GATGCCTTAA AGAGCAGAGC CACAGCCTCC
    AATGCAGAGA AAGTGCCGGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC
    CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT
    GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC
    CTCTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA CGAGCATGGC
    TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC
    CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC
    TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC
    AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC
    ATTGTCTACA ATGACTCTGG TGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC
    TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC
    AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC
    CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCATCAT
    AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC
    AAGAGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG
    GTGCACCCTC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC
    TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024205809.1
    CDS195..5417
    Translation

    Target ORF information:

    RefSeq Version XM_024350041.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024350041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
    CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC
    ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG
    ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA
    CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG
    GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA
    GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG
    GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG
    GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG
    GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTGGACGAGT ACATCGCAAG GATGGTTCAG
    ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC
    GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG
    CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG
    GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCTGTGTT TTTTGTGGGC
    ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG
    CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG
    TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT
    CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC
    AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG
    TCCTCCCTTC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC
    TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA
    GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC
    CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG
    AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA
    AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC
    AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG
    CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC
    AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC
    GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG
    CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG
    TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA
    GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG
    CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG
    GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT
    GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CGGCCACAGC CAGCATGGCC
    GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT
    GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG
    TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC
    CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC
    CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC
    AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT
    GAGAACTCGG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTGACGGAA
    ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT GCCGAAGAGG
    TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC
    TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGTGTGCA TGTGAGACAG
    ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGCG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG
    CCGGCTTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGTG
    AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTATGC AGGAGAAGAC GAACCTGGCG
    GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC
    AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC
    CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT
    CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAT TCCGGGCGAG
    GTTCCTGTGC CGGTGGCGCC CCCAGGGTTG GAGGACGTCG AGGCAGCGCT AGGCATGGAC
    AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC
    CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGCCGTCTC CTCAGAGGGT
    GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC
    TCTCCCGAGC TGCAGACTCT GCAGGACATC CTTGGGGACC CTGGGGACAA GGCCGACGTG
    GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT
    GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC
    AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC
    CCATCACGCA GGGGCAAGAG AGTAGAGAGG GATGCCTTAA AGAGCAGAGC CACAGCCTCC
    AATGCAGAGA AAGTGCCGGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC
    CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT
    GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC
    CTCTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA CGAGCATGGC
    TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC
    CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC
    TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC
    AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC
    ATTGTCTACA ATGACTCTGG TGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC
    TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC
    AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC
    CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCATCAT
    AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC
    AAGAGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG
    GTGCACCCTC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC
    TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.