HECW1 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009452966.2	XP_009451241.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_016957315.2	XP_016812804.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_016957317.2	XP_016812806.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_009452961.2	XP_009451236.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_009452965.2	XP_009451240.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_009452959.3	XP_009451234.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_003318384.4	XP_003318432.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_016957318.2	XP_016812807.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_009452959.2	XP_009451234.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_009452961.3	XP_009451236.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_009452966.3	XP_009451241.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_016957315.1	XP_016812804.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_009452960.2	XP_009451235.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_016957316.1	XP_016812805.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_016957317.1	XP_016812806.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_016957318.1	XP_016812807.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_024357800.1	XP_024213568.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_003318384.3	XP_003318432.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_009452964.2	XP_009451239.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_009452960.3	XP_009451235.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_009452965.3	XP_009451240.1	E3 ubiquitin-protein ligase HECW1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc107914	XM_016957315.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc107915	XM_016957317.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc107916	XM_016957318.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc26511	XM_003318384.4 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40505	XM_009452959.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40506	XM_009452961.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40506	XM_009452960.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40509	XM_024357800.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40510	XM_009452965.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc40511	XM_009452966.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc57238	XM_009452959.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57239	XM_016957315.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57240	XM_009452961.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57240	XM_009452960.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57241	XM_003318384.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57242	XM_009452964.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57242	XM_016957316.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57243	XM_016957317.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57244	XM_016957318.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57245	XM_009452965.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc57246	XM_009452966.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

HECW1 ( XM_016957315.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812804.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc107914
Clone ID Related Accession (Same CDS sequence)	XM_016957315.2
Accession Version	XM_016957315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016957315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812804.1
CDS	359..5182
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957315.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957315.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957317.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812806.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPc107915
Clone ID Related Accession (Same CDS sequence)	XM_016957317.2
Accession Version	XM_016957317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016957317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCGACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCCGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAG TTCTTCACTG TGCTGCACGC CAATTATAGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACTCACCGAC AGCACCTCCA GAGGCTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCCCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC TACCGAAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCGGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AGGTGATGGC CTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTACGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGTTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC TTAGACCTCA CTTTCACTGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACA 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG 
GTACAGCAGA CCGAGGCGCT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CTTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812806.1
CDS	360..5084
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957317.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957317.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCGACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCCGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAG TTCTTCACTG TGCTGCACGC CAATTATAGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACTCACCGAC AGCACCTCCA GAGGCTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCCCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC TACCGAAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCGGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AGGTGATGGC CTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTACGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGTTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC TTAGACCTCA CTTTCACTGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACA 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG 
GTACAGCAGA CCGAGGCGCT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CTTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957318.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812807.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPc107916
Clone ID Related Accession (Same CDS sequence)	XM_016957318.2
Accession Version	XM_016957318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016957318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TGCACGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACAGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812807.1
CDS	360..5081
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957318.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957318.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TGCACGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACAGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_003318384.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003318432.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OPc26511
Clone ID Related Accession (Same CDS sequence)	XM_003318384.4
Accession Version	XM_003318384.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003318384.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003318432.1
CDS	655..5475
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003318384.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003318384.4

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452959.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451234.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OPc40505
Clone ID Related Accession (Same CDS sequence)	XM_009452959.3
Accession Version	XM_009452959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTGCAC GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451234.1
CDS	360..5186
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452959.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452959.3

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTGCAC GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452961.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451236.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OPc40506
Clone ID Related Accession (Same CDS sequence)	XM_009452961.3 , XM_009452960.3
Accession Version	XM_009452961.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451236.1
CDS	679..5502
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452961.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452961.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452960.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451235.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OPc40506
Clone ID Related Accession (Same CDS sequence)	XM_009452961.3 , XM_009452960.3
Accession Version	XM_009452960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451235.1
CDS	421..5244
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452960.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452960.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_024357800.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024213568.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc40509
Clone ID Related Accession (Same CDS sequence)	XM_024357800.1
Accession Version	XM_024357800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024213568.1
CDS	106..4866
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_024357800.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024357800.1

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452965.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451240.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

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CloneID	OPc40510
Clone ID Related Accession (Same CDS sequence)	XM_009452965.3
Accession Version	XM_009452965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTGC ACGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACAGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451240.1
CDS	420..5138
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452965.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452965.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTGC ACGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACAGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452966.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451241.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

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CloneID	OPc40511
Clone ID Related Accession (Same CDS sequence)	XM_009452966.3
Accession Version	XM_009452966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA TGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACAA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA ACAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGACCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAC GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACAG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451241.1
CDS	113..4426
Translation	MAALAEHKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452966.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452966.3

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA TGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACAA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA ACAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGACCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAC GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACAG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452959.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451234.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OPc57238
Clone ID Related Accession (Same CDS sequence)	XM_009452959.2
Accession Version	XM_009452959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCGGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTGCAC GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAATTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451234.1
CDS	360..5186
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452959.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452959.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCGGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTGCAC GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAATTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957315.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812804.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

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CloneID	OPc57239
Clone ID Related Accession (Same CDS sequence)	XM_016957315.1
Accession Version	XM_016957315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812804.1
CDS	360..5183
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957315.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957315.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452961.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451236.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc57240
Clone ID Related Accession (Same CDS sequence)	XM_009452961.2 , XM_009452960.2
Accession Version	XM_009452961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451236.1
CDS	679..5502
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452961.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452961.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452960.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451235.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OPc57240
Clone ID Related Accession (Same CDS sequence)	XM_009452961.2 , XM_009452960.2
Accession Version	XM_009452960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451235.1
CDS	421..5244
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452960.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452960.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTGCACGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_003318384.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003318432.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OPc57241
Clone ID Related Accession (Same CDS sequence)	XM_003318384.3
Accession Version	XM_003318384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003318384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003318432.1
CDS	656..5476
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003318384.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003318384.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957316.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812805.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

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CloneID	OPc57242
Clone ID Related Accession (Same CDS sequence)	XM_016957316.1 , XM_009452964.2
Accession Version	XM_016957316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812805.1
CDS	808..5568
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957316.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957316.1

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452964.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451239.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc57242
Clone ID Related Accession (Same CDS sequence)	XM_016957316.1 , XM_009452964.2
Accession Version	XM_009452964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451239.1
CDS	811..5571
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452964.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452964.2

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGTCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCATACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCG GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT GCACGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACAGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957317.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812806.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPc57243
Clone ID Related Accession (Same CDS sequence)	XM_016957317.1
Accession Version	XM_016957317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCGACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCCGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCGGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAG TTCTTCACTG TGCTGCACGC CAATTATAGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ATTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACTCACCGAC AGCACCTCCA GAGGCTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCCCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC TACCGAAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCGGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AGGTGATGGC CTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTACGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGTTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC TTAGACCTCA CTTTCACTGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACA 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG 
GTACAGCAGA CCGAGGCGCT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CTTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812806.1
CDS	361..5085
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957317.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957317.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCATGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CAACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAACAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGACC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GACGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCGACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCCGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCGGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAG TTCTTCACTG TGCTGCACGC CAATTATAGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ATTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACTCACCGAC AGCACCTCCA GAGGCTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCCCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC TACCGAAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCGGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AGGTGATGGC CTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTACGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGTTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC TTAGACCTCA CTTTCACTGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACA 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG 
GTACAGCAGA CCGAGGCGCT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CTTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_016957318.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812807.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

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CloneID	OPc57244
Clone ID Related Accession (Same CDS sequence)	XM_016957318.1
Accession Version	XM_016957318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC GGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TGCACGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAATT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACAGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812807.1
CDS	360..5081
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_016957318.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016957318.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGTCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA TGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACAA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA ACAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGACCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAC 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC GGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TGCACGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAATT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACAGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452965.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451240.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

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CloneID	OPc57245
Clone ID Related Accession (Same CDS sequence)	XM_009452965.2
Accession Version	XM_009452965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCGGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTGC ACGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAATTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACAGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451240.1
CDS	421..5139
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452965.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452965.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG TCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCATGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACAACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAACA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GACCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGACGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCGGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTGC ACGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAATTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACAGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_009452966.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451241.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

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CloneID	OPc57246
Clone ID Related Accession (Same CDS sequence)	XM_009452966.2
Accession Version	XM_009452966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA TGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACAA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA ACAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGACCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAC GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA TTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACAG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451241.1
CDS	114..4427
Translation	MAALAEHKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSMELSRPAEEAAVIMEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPDHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009452966.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452966.2

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA TGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACAA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA ACAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGACCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAC GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCGGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTGCACGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA TTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACAG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HECW1
Entrez Gene ID	463367
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECW1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HECW1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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