MAP3K1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	427144
Full Name	mitogen-activated protein kinase kinase kinase 1
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 1 Names mitogen-activated protein kinase kinase kinase 1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015277577.1	XP_015133063.1	mitogen-activated protein kinase kinase kinase 1 isoform X3
XM_015277578.1	XP_015133064.1	mitogen-activated protein kinase kinase kinase 1 isoform X3
XM_015277579.1	XP_015133065.1	mitogen-activated protein kinase kinase kinase 1 isoform X3
XM_015277576.1	XP_015133062.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
XM_015277575.1	XP_015133061.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_015277575.2	XP_015133061.1	mitogen-activated protein kinase kinase kinase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K1	NP_005912.1
Rattus norvegicus (Norway rat)	LOC100912399	XP_006231988.1
	MAP3K1	XP_002804414.1
Canis lupus familiaris (dog)	MAP3K1	XP_535240.4
Xenopus tropicalis (tropical clawed frog)	map3k1	XP_002940279.2
Rattus norvegicus (Norway rat)	Map3k1	NP_446339.1
Pan troglodytes (chimpanzee)	MAP3K1	XP_003310777.1
Bos taurus (cattle)	MAP3K1	NP_001192835.1
Mus musculus (house mouse)	Map3k1	NP_036075.2
Gallus gallus (chicken)	MAP3K1	XP_424734.3
Danio rerio (zebrafish)	map3k1	XP_002663531.2

Gene Ontology

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa47869	XM_015277576.1 Latest version!	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47870	XM_015277577.1 Latest version!	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47870	XM_015277578.1 Latest version!	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47870	XM_015277579.1 Latest version!	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47868	XM_015277575.2 Latest version!	Gallus gallus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OGa47868	XM_015277575.1	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_015277576.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133062.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa47869
Clone ID Related Accession (Same CDS sequence)	XM_015277576.1
Accession Version	XM_015277576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGG TTTTTAGATA CAGCCTAGAT GCAAACAAGG AGGTGAAAGT TTACAGCAGA 
TCAGAGATCA ACTGGGGCAA ACTCATGCAG AGGCATCCTC CTCTGATTCC CCCCTGGAGC 
AACCAATTGT TGGTAAAAGA GAGGAAGAAT TGTCGTGACA TGGAGAATAA AGAAACCCTC 
AGGGGGTTGC AGAAGATGGA TGACCGCCCA GAAGAGCGCA TGATCAGGGA GAAACTCAAG 
GCAACGTGTA TGCCAGCTTG GAAGCATGAA TGGCTGGAAA GAAGAAGCAG GAGAGGCCCT 
GTGGTGGTGA AACCTATCCC TGTGAAGGGA GATGGATCTG AAATGAACAA GCTATCTCTA 
GAACCTCAAT CTGAAGGACA AAGCTCTGCT TCTTCACCTA CGCAGAAAGG AAGGCGTAGT 
CCTTCTCCTA GCAGTTCTTC TTCATCATCC TCTCGGACTG TTAAATCTGA GTCACCAGGT 
GTTAGAAGAA AAAGGGTATC TCCAGTACCT TTTCAGAGTG GACGAATAAC ACCACCTCGA 
AGAGCCCCAT CTCCAGATGG TTTCTCTCCA TACAGCCCTG AGGAAACAAG TCGTCGTGTC 
AACAAAGTTA TGCGAGCCAG GCTGTACCTG CTGCAGCAGA TAGGACCCAA CTCTTTCTTA 
ATTGGAGGAG ATAGCCCTGA TAATAAATAC AGAGTGTTTA TTGGACCTCA GACATGTAGC 
TGTGGCCGTG GAACATTTTG TATTCATCTG CTCTTTGTTA TGCTGCGAGT GTTCCAGCTT 
GAGCCTTCAG ACCCAATGCT GTGGAGAAAG ACACTGAAAA ACTTTGAGGT TGAGAGTTTG 
TTCCAGAAAT ATCACAGTAG GCGTAGCTCG AGGATCAAAG CTCCATCTCG TAACACCATC 
CAGAAGTTTG TCTCACGCAT GTCAAATTCT CATACATTAT CATCTAGTAC TTCTACATCT 
AGTTCAGAAA ACAGTATGAA AGATGAAGAG GAGCAGATGT GCCCCATTTG TTTGCTGGGC 
ATGCTGGATG AAGAGAGCCT GACTGTGTGT GAGGATGGCT GCAGGAACAA ATTACACCAC 
CACTGCATGT CAATATGGGC AGAAGAGTGT AGAAGAAACA GAGAGCCACT TATATGTCCT 
CTGTGTAGAT CTAAATGGAG ATCTCATGAT TTCTACAGTC ATGAATTGCC AAGCCCTGTG 
GATTCTTCTG TTCTCCATGT GGTTCAACAA CAAACTCAAC AGCAATCAAC AATTGGATCA 
CAGAGGAGAA CCCAGGATAG CAATTTCAAC CTTACTCATT ATGGGGTCCA GCAGATTCCT 
TCTGCCTATA AAGATTTAGC TGAGCCATGG ATTCAGGTCT TTGGAATGGA GTTAGTTGGC 
TGCTTGTTTT CTCGAAACTG GAATATACGA GAGATGGCAC TTAGACGTCT TTCCCATGAT 
GTTAGTGGTG CTTTATTACT GGCTAATGGT GAAAGCACTG GAAGTTCTGG AAATAGCAAT 
GGAAACAACA CGAGTGCTGG AGCTCTGGGA ATAGCGAGTG GGTCATCTCA GGCCACCGTC 
TCAGGAGATG TTGTGGTGGA ATCTTGCTGC AGTGTGCTAT CCATGGTCTG TGCTGACCCT 
GTCTACAAAG TGTATGTTGC TGCTTTAAAA ACCTTAAGAG CCATGCTGGT CTATACTCCT 
TGTCATAGTT TGGCAGAAAG GACTAAACTA CAGCGACTTC TGAAGCCAGT TGTAGAGACA 
ATATTAGTTA AATGTGCAGA TGCCAACAGT CGAACAAGTC AACTTTCAGT CTCAACGCTA 
TTGGAGATGT GTAAAGGCCA AGCAGGAGAG TTGGCAGTTG GGAGAGAAAT ACTTAAATCT 
GGGTCCATTG GTATTGGTGG TGTTGATTAT GTCTTAAATT GTATTCTTGC AACTGAAATT 
GAATCAAGCA ACTGGCAAGC GCTACTGGGG AGACTTTGTC TTATAGACAG ACTTCTGTTG 
GAATTTCCTG GTGAATTTTA TCCTCACATT GTCTGTGGAG ATGTTTTACA GGCTGATGCC 
ATAGTAGGCA GATATAAGAA ACTTCTCTCC CTTCTAAATT TCGCCTTGCT GTCAATTGAC 
AATTCCCACT CAATGGTTGG TAAACTATCA CGGAGAGTAT TTTTGAGTTC AGCAAGAATG 
GTTGCAAGAG TTCCTCACGT GTTTGTAAAG CTGTTAGAAA TGTTGAGTGT GACAAGCTCA 
ACTCATTACA CAAGGATGCG TCGACGTCTG ATGGCAATAG CAGATGAAAT GGAAGTTGCA 
GAAGCCATCC AGCTTGGCAT GGAAGAAATA CAGTCTGGGG AATGTCGACA TGAAGACTTT 
CTGCAGCTAC CCGTACCAGA TAATTCTCCA GAAGCTACAG AAAATAATAC TCCTAACAAT 
ACTGTCCAGT TATCAGGGAA AAATGGGAAA GGCTTAAGTG ACAAAAAACT GAGTGCTGGT 
CCAGAGGACA TTTCTGAGAC ACTGACTAGC CTAGTTGTGG GACATCCTGT TTCATCACTA 
ACCACTGAGC AACCAAAGCC AGCTGTTCAA ACAAAAGGAA AACCCTACAG CCAGTGTTTG 
AACTCTTCTC CCTCATCCAG TCATTCCCAG TCACTATTCC CAACACTTCT CTCTCCTTCA 
AACCCATCTG TATCAGCTGG CACTGTAACA GATGTCTCTA AACTCCGACC TCAGGGATTC 
ATTCCCTGCA AAATCCCTTC TCCTTCTCCT CAAACACAAC GGAAGCTTTC CCTCCAGTTT 
CAGAGAAGCT GCTCTGAAAA TAAAGAACCA GATAAGCTTT CTCCAGTTTT TACCCAAGCC 
AGGCCATTGC CATCTAGTCA CATACACAGG CCAAAGCCAT CACGACCCAC TCCAAATGAT 
ATGAACAAGC AGGGAGAAAC TTCAAAGAAC AGCATGACAC TTGACCTGAA TGATACTTCA 
CATTGTGATG GCAATGGTAG TAACAGTAGT GCAGTTATAC CAAGTGAAGA GACAGTGTTC 
ACACCAGTAG ATGAAAAATG TCGGCTGGAT GTTAATGCAG AGCTCAACTC CAGTATTGAG 
GACCTCCTTG AGGCCTCCAT GCCAACAAGT GATGGCACAG TTACTTTCAA GTCCGAAGTT 
GCAGTTCTTT CTCCCGAGAG GGCTGAAAAT GATGATACTT ACAAAGATGA TGTAAATCAT 
AATCAAAAAT GCAAGGAAAA GATGGAAGCT GAAGAAGAGG AAGCTTTAGC TATTGCTATG 
GCAATGTCTG CATCTCAAGA TGCCCTGCCA ATAATTCCCC AACTACAGGT CGAAAATGGT 
GAAGATATCA TAATTATTCA GCAAGATACA CCAGAAACTC TGCCTGGACA TACCAAAGCA 
AAGCATCATT ACAGGGAAGA TGCAGAATGG CTTAAAGGTC AGCAAATTGG TCTTGGAGCT 
TTCTCTTCCT GTTACCAAGC TCAAGATGTA GGAACAGGGA CATTAATGGC TGTAAAACAG 
GTGACATATG TCAGGAACAC ATCATCTGAG CAAGAAGAGG TAGTGGAAGC ACTGAGGGAG 
GAAATACGGA TGATGAGTCA TCTAAACCAT CCTAATATTA TTCGCATGTT GGGTGCTACA 
TGTGAGAAGA GCAACTACAA CCTCTTCATT GAATGGATGG CAGGGGGATC AGTTGCTCAT 
TTGTTGAGTA AATACGGAGC CTTCAAAGAA TCAGTTATTA TTAACTACAC AGAGCAACTG 
TTACGTGGCC TTTCTTACCT CCATGAGAAT CAGATAATTC ATAGAGATGT CAAAGGTGCC 
AATTTGCTAA TTGACAGCAC AGGTCATAGA TTAAGAATTG CTGATTTTGG AGCTGCAGCC 
AGGTTGGCAT CAAAAGGAAC TGGTGCTGGG GAGTTTCAGG GACAGTTGTT GGGAACTATT 
GCATTTATGG CCCCGGAGGT TCTGAGAGGT CAGCAGTATG GTAGGAGCTG TGATGTGTGG 
AGCGTTGGCT GTGTTGTCAT AGAAATGGCT TGTGCTAAAC CTCCCTGGAA TGCAGAGAAA 
CACTCCAATC ATCTTGCTCT GATCTTTAAG ATTGCTAGTG CAACTACTGC TCCATCAATC 
CCTTCACATC TGTCTCCTGG CTTGAGGGAT GTGACTCTTC GGTGTTTAGA ACTTCAACCT 
CAGGACAGAC CCCCATCGAG GGAGCTGCTG AAACACCCAG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133062.1
CDS	16..4215
Translation	MLWVFRYSLDANKEVKVYSRSEINWGKLMQRHPPLIPPWSNQLLVKERKNCRDMENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277576.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277576.1

ORF Insert Sequence:

ATGCTGTGGG TTTTTAGATA CAGCCTAGAT GCAAACAAGG AGGTGAAAGT TTACAGCAGA 
TCAGAGATCA ACTGGGGCAA ACTCATGCAG AGGCATCCTC CTCTGATTCC CCCCTGGAGC 
AACCAATTGT TGGTAAAAGA GAGGAAGAAT TGTCGTGACA TGGAGAATAA AGAAACCCTC 
AGGGGGTTGC AGAAGATGGA TGACCGCCCA GAAGAGCGCA TGATCAGGGA GAAACTCAAG 
GCAACGTGTA TGCCAGCTTG GAAGCATGAA TGGCTGGAAA GAAGAAGCAG GAGAGGCCCT 
GTGGTGGTGA AACCTATCCC TGTGAAGGGA GATGGATCTG AAATGAACAA GCTATCTCTA 
GAACCTCAAT CTGAAGGACA AAGCTCTGCT TCTTCACCTA CGCAGAAAGG AAGGCGTAGT 
CCTTCTCCTA GCAGTTCTTC TTCATCATCC TCTCGGACTG TTAAATCTGA GTCACCAGGT 
GTTAGAAGAA AAAGGGTATC TCCAGTACCT TTTCAGAGTG GACGAATAAC ACCACCTCGA 
AGAGCCCCAT CTCCAGATGG TTTCTCTCCA TACAGCCCTG AGGAAACAAG TCGTCGTGTC 
AACAAAGTTA TGCGAGCCAG GCTGTACCTG CTGCAGCAGA TAGGACCCAA CTCTTTCTTA 
ATTGGAGGAG ATAGCCCTGA TAATAAATAC AGAGTGTTTA TTGGACCTCA GACATGTAGC 
TGTGGCCGTG GAACATTTTG TATTCATCTG CTCTTTGTTA TGCTGCGAGT GTTCCAGCTT 
GAGCCTTCAG ACCCAATGCT GTGGAGAAAG ACACTGAAAA ACTTTGAGGT TGAGAGTTTG 
TTCCAGAAAT ATCACAGTAG GCGTAGCTCG AGGATCAAAG CTCCATCTCG TAACACCATC 
CAGAAGTTTG TCTCACGCAT GTCAAATTCT CATACATTAT CATCTAGTAC TTCTACATCT 
AGTTCAGAAA ACAGTATGAA AGATGAAGAG GAGCAGATGT GCCCCATTTG TTTGCTGGGC 
ATGCTGGATG AAGAGAGCCT GACTGTGTGT GAGGATGGCT GCAGGAACAA ATTACACCAC 
CACTGCATGT CAATATGGGC AGAAGAGTGT AGAAGAAACA GAGAGCCACT TATATGTCCT 
CTGTGTAGAT CTAAATGGAG ATCTCATGAT TTCTACAGTC ATGAATTGCC AAGCCCTGTG 
GATTCTTCTG TTCTCCATGT GGTTCAACAA CAAACTCAAC AGCAATCAAC AATTGGATCA 
CAGAGGAGAA CCCAGGATAG CAATTTCAAC CTTACTCATT ATGGGGTCCA GCAGATTCCT 
TCTGCCTATA AAGATTTAGC TGAGCCATGG ATTCAGGTCT TTGGAATGGA GTTAGTTGGC 
TGCTTGTTTT CTCGAAACTG GAATATACGA GAGATGGCAC TTAGACGTCT TTCCCATGAT 
GTTAGTGGTG CTTTATTACT GGCTAATGGT GAAAGCACTG GAAGTTCTGG AAATAGCAAT 
GGAAACAACA CGAGTGCTGG AGCTCTGGGA ATAGCGAGTG GGTCATCTCA GGCCACCGTC 
TCAGGAGATG TTGTGGTGGA ATCTTGCTGC AGTGTGCTAT CCATGGTCTG TGCTGACCCT 
GTCTACAAAG TGTATGTTGC TGCTTTAAAA ACCTTAAGAG CCATGCTGGT CTATACTCCT 
TGTCATAGTT TGGCAGAAAG GACTAAACTA CAGCGACTTC TGAAGCCAGT TGTAGAGACA 
ATATTAGTTA AATGTGCAGA TGCCAACAGT CGAACAAGTC AACTTTCAGT CTCAACGCTA 
TTGGAGATGT GTAAAGGCCA AGCAGGAGAG TTGGCAGTTG GGAGAGAAAT ACTTAAATCT 
GGGTCCATTG GTATTGGTGG TGTTGATTAT GTCTTAAATT GTATTCTTGC AACTGAAATT 
GAATCAAGCA ACTGGCAAGC GCTACTGGGG AGACTTTGTC TTATAGACAG ACTTCTGTTG 
GAATTTCCTG GTGAATTTTA TCCTCACATT GTCTGTGGAG ATGTTTTACA GGCTGATGCC 
ATAGTAGGCA GATATAAGAA ACTTCTCTCC CTTCTAAATT TCGCCTTGCT GTCAATTGAC 
AATTCCCACT CAATGGTTGG TAAACTATCA CGGAGAGTAT TTTTGAGTTC AGCAAGAATG 
GTTGCAAGAG TTCCTCACGT GTTTGTAAAG CTGTTAGAAA TGTTGAGTGT GACAAGCTCA 
ACTCATTACA CAAGGATGCG TCGACGTCTG ATGGCAATAG CAGATGAAAT GGAAGTTGCA 
GAAGCCATCC AGCTTGGCAT GGAAGAAATA CAGTCTGGGG AATGTCGACA TGAAGACTTT 
CTGCAGCTAC CCGTACCAGA TAATTCTCCA GAAGCTACAG AAAATAATAC TCCTAACAAT 
ACTGTCCAGT TATCAGGGAA AAATGGGAAA GGCTTAAGTG ACAAAAAACT GAGTGCTGGT 
CCAGAGGACA TTTCTGAGAC ACTGACTAGC CTAGTTGTGG GACATCCTGT TTCATCACTA 
ACCACTGAGC AACCAAAGCC AGCTGTTCAA ACAAAAGGAA AACCCTACAG CCAGTGTTTG 
AACTCTTCTC CCTCATCCAG TCATTCCCAG TCACTATTCC CAACACTTCT CTCTCCTTCA 
AACCCATCTG TATCAGCTGG CACTGTAACA GATGTCTCTA AACTCCGACC TCAGGGATTC 
ATTCCCTGCA AAATCCCTTC TCCTTCTCCT CAAACACAAC GGAAGCTTTC CCTCCAGTTT 
CAGAGAAGCT GCTCTGAAAA TAAAGAACCA GATAAGCTTT CTCCAGTTTT TACCCAAGCC 
AGGCCATTGC CATCTAGTCA CATACACAGG CCAAAGCCAT CACGACCCAC TCCAAATGAT 
ATGAACAAGC AGGGAGAAAC TTCAAAGAAC AGCATGACAC TTGACCTGAA TGATACTTCA 
CATTGTGATG GCAATGGTAG TAACAGTAGT GCAGTTATAC CAAGTGAAGA GACAGTGTTC 
ACACCAGTAG ATGAAAAATG TCGGCTGGAT GTTAATGCAG AGCTCAACTC CAGTATTGAG 
GACCTCCTTG AGGCCTCCAT GCCAACAAGT GATGGCACAG TTACTTTCAA GTCCGAAGTT 
GCAGTTCTTT CTCCCGAGAG GGCTGAAAAT GATGATACTT ACAAAGATGA TGTAAATCAT 
AATCAAAAAT GCAAGGAAAA GATGGAAGCT GAAGAAGAGG AAGCTTTAGC TATTGCTATG 
GCAATGTCTG CATCTCAAGA TGCCCTGCCA ATAATTCCCC AACTACAGGT CGAAAATGGT 
GAAGATATCA TAATTATTCA GCAAGATACA CCAGAAACTC TGCCTGGACA TACCAAAGCA 
AAGCATCATT ACAGGGAAGA TGCAGAATGG CTTAAAGGTC AGCAAATTGG TCTTGGAGCT 
TTCTCTTCCT GTTACCAAGC TCAAGATGTA GGAACAGGGA CATTAATGGC TGTAAAACAG 
GTGACATATG TCAGGAACAC ATCATCTGAG CAAGAAGAGG TAGTGGAAGC ACTGAGGGAG 
GAAATACGGA TGATGAGTCA TCTAAACCAT CCTAATATTA TTCGCATGTT GGGTGCTACA 
TGTGAGAAGA GCAACTACAA CCTCTTCATT GAATGGATGG CAGGGGGATC AGTTGCTCAT 
TTGTTGAGTA AATACGGAGC CTTCAAAGAA TCAGTTATTA TTAACTACAC AGAGCAACTG 
TTACGTGGCC TTTCTTACCT CCATGAGAAT CAGATAATTC ATAGAGATGT CAAAGGTGCC 
AATTTGCTAA TTGACAGCAC AGGTCATAGA TTAAGAATTG CTGATTTTGG AGCTGCAGCC 
AGGTTGGCAT CAAAAGGAAC TGGTGCTGGG GAGTTTCAGG GACAGTTGTT GGGAACTATT 
GCATTTATGG CCCCGGAGGT TCTGAGAGGT CAGCAGTATG GTAGGAGCTG TGATGTGTGG 
AGCGTTGGCT GTGTTGTCAT AGAAATGGCT TGTGCTAAAC CTCCCTGGAA TGCAGAGAAA 
CACTCCAATC ATCTTGCTCT GATCTTTAAG ATTGCTAGTG CAACTACTGC TCCATCAATC 
CCTTCACATC TGTCTCCTGG CTTGAGGGAT GTGACTCTTC GGTGTTTAGA ACTTCAACCT 
CAGGACAGAC CCCCATCGAG GGAGCTGCTG AAACACCCAG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_015277577.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133063.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa47870
Clone ID Related Accession (Same CDS sequence)	XM_015277577.1 , XM_015277578.1 , XM_015277579.1
Accession Version	XM_015277577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133063.1
CDS	198..4238
Translation	MENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277577.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277577.1

ORF Insert Sequence:

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_015277578.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133064.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

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CloneID	OGa47870
Clone ID Related Accession (Same CDS sequence)	XM_015277577.1 , XM_015277578.1 , XM_015277579.1
Accession Version	XM_015277578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133064.1
CDS	440..4480
Translation	MENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277578.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277578.1

ORF Insert Sequence:

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_015277579.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133065.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X5, mRNA.

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CloneID	OGa47870
Clone ID Related Accession (Same CDS sequence)	XM_015277577.1 , XM_015277578.1 , XM_015277579.1
Accession Version	XM_015277579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133065.1
CDS	182..4222
Translation	MENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277579.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277579.1

ORF Insert Sequence:

ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA 
AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT 
GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT 
ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT 
GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT 
GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT 
GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG 
ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT 
ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT 
ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA 
AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA 
GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG 
TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC 
TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC 
AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT 
CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA 
CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT 
TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC 
TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA 
CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT 
GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT 
GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA 
TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA 
GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT 
CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT 
CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT 
CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA 
GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT 
TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA 
TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA 
ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA 
GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG 
GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA 
GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT 
GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG 
GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA 
AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC 
CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT 
AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA 
CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT 
TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA 
TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA 
CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA 
CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA 
GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA 
GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT 
TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG 
GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC 
CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT 
CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT 
CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG 
ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG 
GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT 
ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG 
GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT 
ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT 
CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT 
GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG 
GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA 
CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT 
GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT 
CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_015277575.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133061.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa47868
Clone ID Related Accession (Same CDS sequence)	XM_015277575.1 , XM_015277575.2
Accession Version	XM_015277575.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC 
CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG 
TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC 
CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG 
GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG 
CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC 
GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG 
CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC 
GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG 
AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG 
TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG 
TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG 
GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT 
CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT 
CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA 
AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC 
CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA 
GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA 
GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC 
CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT 
TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG 
AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG 
TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG 
GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC 
ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT 
AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT 
TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG 
AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC 
TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG 
TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT 
GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC 
AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA 
GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC 
AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT 
AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA 
GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG 
ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC 
ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA 
AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT 
CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA 
GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC 
CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA 
AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT 
TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC 
ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG 
CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC 
CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG 
GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT 
GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT 
TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA 
TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC 
TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA 
AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA 
TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC 
AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT 
GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA 
GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC 
CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT 
CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA 
AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG 
TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT 
ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT 
CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT 
TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA 
TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA 
CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG 
AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG 
AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT 
GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT 
ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT 
GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC 
AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA 
CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC 
AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133061.1
CDS	32..4705
Translation	MGARRPTPSLRRLRRQRRFRSRRERRLPPPSLPPSAPARVPGARCDPPEKMAAAAGNRTSSSGLSSGGGAEGAAAAACGSPKGGAGEGSGAGSGGLLREAGGGGGREQRSDWRRKQLRKVRSVELDRLPEAPLLLAAAPAAASSSSSSSPSPEPPEALLLHYLPGPRSGPASPAVSPGRGAELLELPAGSPAAADPAAERRMEPSPAASRDMENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277575.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277575.1

ORF Insert Sequence:

ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC 
CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG 
TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC 
CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG 
GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG 
CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC 
GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG 
CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC 
GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG 
AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG 
TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG 
TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG 
GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT 
CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT 
CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA 
AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC 
CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA 
GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA 
GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC 
CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT 
TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG 
AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG 
TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG 
GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC 
ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT 
AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT 
TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG 
AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC 
TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG 
TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT 
GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC 
AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA 
GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC 
AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT 
AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA 
GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG 
ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC 
ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA 
AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT 
CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA 
GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC 
CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA 
AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT 
TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC 
ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG 
CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC 
CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG 
GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT 
GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT 
TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA 
TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC 
TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA 
AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA 
TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC 
AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT 
GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA 
GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC 
CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT 
CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA 
AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG 
TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT 
ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT 
CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT 
TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA 
TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA 
CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG 
AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG 
AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT 
GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT 
ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT 
GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC 
AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA 
CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC 
AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_015277575.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133061.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

Price & Availability↑

CloneID	OGa47868
Clone ID Related Accession (Same CDS sequence)	XM_015277575.1 , XM_015277575.2
Accession Version	XM_015277575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC 
CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG 
TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC 
CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG 
GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG 
CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC 
GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG 
CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC 
GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG 
AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG 
TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG 
TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG 
GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT 
CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT 
CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA 
AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC 
CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA 
GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA 
GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC 
CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT 
TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG 
AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG 
TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG 
GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC 
ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT 
AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT 
TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG 
AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC 
TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG 
TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT 
GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC 
AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA 
GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC 
AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT 
AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA 
GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG 
ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC 
ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA 
AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT 
CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA 
GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC 
CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA 
AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT 
TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC 
ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG 
CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC 
CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG 
GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT 
GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT 
TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA 
TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC 
TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA 
AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA 
TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC 
AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT 
GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA 
GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC 
CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT 
CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA 
AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG 
TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT 
ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT 
CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT 
TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA 
TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA 
CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG 
AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG 
AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT 
GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT 
ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT 
GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC 
AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA 
CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC 
AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133061.1
CDS	394..5067
Translation	MGARRPTPSLRRLRRQRRFRSRRERRLPPPSLPPSAPARVPGARCDPPEKMAAAAGNRTSSSGLSSGGGAEGAAAAACGSPKGGAGEGSGAGSGGLLREAGGGGGREQRSDWRRKQLRKVRSVELDRLPEAPLLLAAAPAAASSSSSSSPSPEPPEALLLHYLPGPRSGPASPAVSPGRGAELLELPAGSPAAADPAAERRMEPSPAASRDMENKETLRGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEMNKLSLEPQSEGQSSASSPTQKGRRSPSPSSSSSSSSRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSSVLHVVQQQTQQQSTIGSQRRTQDSNFNLTHYGVQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGSSGNSNGNNTSAGALGIASGSSQATVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERTKLQRLLKPVVETILVKCADANSRTSQLSVSTLLEMCKGQAGELAVGREILKSGSIGIGGVDYVLNCILATEIESSNWQALLGRLCLIDRLLLEFPGEFYPHIVCGDVLQADAIVGRYKKLLSLLNFALLSIDNSHSMVGKLSRRVFLSSARMVARVPHVFVKLLEMLSVTSSTHYTRMRRRLMAIADEMEVAEAIQLGMEEIQSGECRHEDFLQLPVPDNSPEATENNTPNNTVQLSGKNGKGLSDKKLSAGPEDISETLTSLVVGHPVSSLTTEQPKPAVQTKGKPYSQCLNSSPSSSHSQSLFPTLLSPSNPSVSAGTVTDVSKLRPQGFIPCKIPSPSPQTQRKLSLQFQRSCSENKEPDKLSPVFTQARPLPSSHIHRPKPSRPTPNDMNKQGETSKNSMTLDLNDTSHCDGNGSNSSAVIPSEETVFTPVDEKCRLDVNAELNSSIEDLLEASMPTSDGTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIIPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVIINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGHRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCVVIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_015277575.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277575.2

ORF Insert Sequence:

ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC 
CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG 
TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC 
CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG 
GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG 
CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC 
GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG 
CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC 
GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG 
AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG 
TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG 
TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG 
GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT 
CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT 
CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA 
AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC 
CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA 
GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA 
GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC 
CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT 
TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG 
AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG 
TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG 
GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC 
ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT 
AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT 
TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG 
AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC 
TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG 
TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT 
GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC 
AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA 
GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC 
AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT 
AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA 
GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG 
ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC 
ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA 
AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT 
CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA 
GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC 
CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA 
AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT 
TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC 
ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG 
CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC 
CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG 
GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT 
GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT 
TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA 
TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC 
TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA 
AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA 
TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC 
AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT 
GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA 
GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC 
CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT 
CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA 
AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG 
TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT 
ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT 
CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT 
TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA 
TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA 
CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG 
AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG 
AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT 
GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT 
ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT 
GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC 
AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA 
CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC 
AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAP3K1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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