MAP3K1 cDNA ORF clone, Gallus gallus(chicken)

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa47869 XM_015277576.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47870 XM_015277577.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47870 XM_015277578.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47870 XM_015277579.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47868 XM_015277575.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OGa47868 XM_015277575.1 Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa47869
    Clone ID Related Accession (Same CDS sequence) XM_015277576.1
    Accession Version XM_015277576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGTGGG TTTTTAGATA CAGCCTAGAT GCAAACAAGG AGGTGAAAGT TTACAGCAGA 
    TCAGAGATCA ACTGGGGCAA ACTCATGCAG AGGCATCCTC CTCTGATTCC CCCCTGGAGC
    AACCAATTGT TGGTAAAAGA GAGGAAGAAT TGTCGTGACA TGGAGAATAA AGAAACCCTC
    AGGGGGTTGC AGAAGATGGA TGACCGCCCA GAAGAGCGCA TGATCAGGGA GAAACTCAAG
    GCAACGTGTA TGCCAGCTTG GAAGCATGAA TGGCTGGAAA GAAGAAGCAG GAGAGGCCCT
    GTGGTGGTGA AACCTATCCC TGTGAAGGGA GATGGATCTG AAATGAACAA GCTATCTCTA
    GAACCTCAAT CTGAAGGACA AAGCTCTGCT TCTTCACCTA CGCAGAAAGG AAGGCGTAGT
    CCTTCTCCTA GCAGTTCTTC TTCATCATCC TCTCGGACTG TTAAATCTGA GTCACCAGGT
    GTTAGAAGAA AAAGGGTATC TCCAGTACCT TTTCAGAGTG GACGAATAAC ACCACCTCGA
    AGAGCCCCAT CTCCAGATGG TTTCTCTCCA TACAGCCCTG AGGAAACAAG TCGTCGTGTC
    AACAAAGTTA TGCGAGCCAG GCTGTACCTG CTGCAGCAGA TAGGACCCAA CTCTTTCTTA
    ATTGGAGGAG ATAGCCCTGA TAATAAATAC AGAGTGTTTA TTGGACCTCA GACATGTAGC
    TGTGGCCGTG GAACATTTTG TATTCATCTG CTCTTTGTTA TGCTGCGAGT GTTCCAGCTT
    GAGCCTTCAG ACCCAATGCT GTGGAGAAAG ACACTGAAAA ACTTTGAGGT TGAGAGTTTG
    TTCCAGAAAT ATCACAGTAG GCGTAGCTCG AGGATCAAAG CTCCATCTCG TAACACCATC
    CAGAAGTTTG TCTCACGCAT GTCAAATTCT CATACATTAT CATCTAGTAC TTCTACATCT
    AGTTCAGAAA ACAGTATGAA AGATGAAGAG GAGCAGATGT GCCCCATTTG TTTGCTGGGC
    ATGCTGGATG AAGAGAGCCT GACTGTGTGT GAGGATGGCT GCAGGAACAA ATTACACCAC
    CACTGCATGT CAATATGGGC AGAAGAGTGT AGAAGAAACA GAGAGCCACT TATATGTCCT
    CTGTGTAGAT CTAAATGGAG ATCTCATGAT TTCTACAGTC ATGAATTGCC AAGCCCTGTG
    GATTCTTCTG TTCTCCATGT GGTTCAACAA CAAACTCAAC AGCAATCAAC AATTGGATCA
    CAGAGGAGAA CCCAGGATAG CAATTTCAAC CTTACTCATT ATGGGGTCCA GCAGATTCCT
    TCTGCCTATA AAGATTTAGC TGAGCCATGG ATTCAGGTCT TTGGAATGGA GTTAGTTGGC
    TGCTTGTTTT CTCGAAACTG GAATATACGA GAGATGGCAC TTAGACGTCT TTCCCATGAT
    GTTAGTGGTG CTTTATTACT GGCTAATGGT GAAAGCACTG GAAGTTCTGG AAATAGCAAT
    GGAAACAACA CGAGTGCTGG AGCTCTGGGA ATAGCGAGTG GGTCATCTCA GGCCACCGTC
    TCAGGAGATG TTGTGGTGGA ATCTTGCTGC AGTGTGCTAT CCATGGTCTG TGCTGACCCT
    GTCTACAAAG TGTATGTTGC TGCTTTAAAA ACCTTAAGAG CCATGCTGGT CTATACTCCT
    TGTCATAGTT TGGCAGAAAG GACTAAACTA CAGCGACTTC TGAAGCCAGT TGTAGAGACA
    ATATTAGTTA AATGTGCAGA TGCCAACAGT CGAACAAGTC AACTTTCAGT CTCAACGCTA
    TTGGAGATGT GTAAAGGCCA AGCAGGAGAG TTGGCAGTTG GGAGAGAAAT ACTTAAATCT
    GGGTCCATTG GTATTGGTGG TGTTGATTAT GTCTTAAATT GTATTCTTGC AACTGAAATT
    GAATCAAGCA ACTGGCAAGC GCTACTGGGG AGACTTTGTC TTATAGACAG ACTTCTGTTG
    GAATTTCCTG GTGAATTTTA TCCTCACATT GTCTGTGGAG ATGTTTTACA GGCTGATGCC
    ATAGTAGGCA GATATAAGAA ACTTCTCTCC CTTCTAAATT TCGCCTTGCT GTCAATTGAC
    AATTCCCACT CAATGGTTGG TAAACTATCA CGGAGAGTAT TTTTGAGTTC AGCAAGAATG
    GTTGCAAGAG TTCCTCACGT GTTTGTAAAG CTGTTAGAAA TGTTGAGTGT GACAAGCTCA
    ACTCATTACA CAAGGATGCG TCGACGTCTG ATGGCAATAG CAGATGAAAT GGAAGTTGCA
    GAAGCCATCC AGCTTGGCAT GGAAGAAATA CAGTCTGGGG AATGTCGACA TGAAGACTTT
    CTGCAGCTAC CCGTACCAGA TAATTCTCCA GAAGCTACAG AAAATAATAC TCCTAACAAT
    ACTGTCCAGT TATCAGGGAA AAATGGGAAA GGCTTAAGTG ACAAAAAACT GAGTGCTGGT
    CCAGAGGACA TTTCTGAGAC ACTGACTAGC CTAGTTGTGG GACATCCTGT TTCATCACTA
    ACCACTGAGC AACCAAAGCC AGCTGTTCAA ACAAAAGGAA AACCCTACAG CCAGTGTTTG
    AACTCTTCTC CCTCATCCAG TCATTCCCAG TCACTATTCC CAACACTTCT CTCTCCTTCA
    AACCCATCTG TATCAGCTGG CACTGTAACA GATGTCTCTA AACTCCGACC TCAGGGATTC
    ATTCCCTGCA AAATCCCTTC TCCTTCTCCT CAAACACAAC GGAAGCTTTC CCTCCAGTTT
    CAGAGAAGCT GCTCTGAAAA TAAAGAACCA GATAAGCTTT CTCCAGTTTT TACCCAAGCC
    AGGCCATTGC CATCTAGTCA CATACACAGG CCAAAGCCAT CACGACCCAC TCCAAATGAT
    ATGAACAAGC AGGGAGAAAC TTCAAAGAAC AGCATGACAC TTGACCTGAA TGATACTTCA
    CATTGTGATG GCAATGGTAG TAACAGTAGT GCAGTTATAC CAAGTGAAGA GACAGTGTTC
    ACACCAGTAG ATGAAAAATG TCGGCTGGAT GTTAATGCAG AGCTCAACTC CAGTATTGAG
    GACCTCCTTG AGGCCTCCAT GCCAACAAGT GATGGCACAG TTACTTTCAA GTCCGAAGTT
    GCAGTTCTTT CTCCCGAGAG GGCTGAAAAT GATGATACTT ACAAAGATGA TGTAAATCAT
    AATCAAAAAT GCAAGGAAAA GATGGAAGCT GAAGAAGAGG AAGCTTTAGC TATTGCTATG
    GCAATGTCTG CATCTCAAGA TGCCCTGCCA ATAATTCCCC AACTACAGGT CGAAAATGGT
    GAAGATATCA TAATTATTCA GCAAGATACA CCAGAAACTC TGCCTGGACA TACCAAAGCA
    AAGCATCATT ACAGGGAAGA TGCAGAATGG CTTAAAGGTC AGCAAATTGG TCTTGGAGCT
    TTCTCTTCCT GTTACCAAGC TCAAGATGTA GGAACAGGGA CATTAATGGC TGTAAAACAG
    GTGACATATG TCAGGAACAC ATCATCTGAG CAAGAAGAGG TAGTGGAAGC ACTGAGGGAG
    GAAATACGGA TGATGAGTCA TCTAAACCAT CCTAATATTA TTCGCATGTT GGGTGCTACA
    TGTGAGAAGA GCAACTACAA CCTCTTCATT GAATGGATGG CAGGGGGATC AGTTGCTCAT
    TTGTTGAGTA AATACGGAGC CTTCAAAGAA TCAGTTATTA TTAACTACAC AGAGCAACTG
    TTACGTGGCC TTTCTTACCT CCATGAGAAT CAGATAATTC ATAGAGATGT CAAAGGTGCC
    AATTTGCTAA TTGACAGCAC AGGTCATAGA TTAAGAATTG CTGATTTTGG AGCTGCAGCC
    AGGTTGGCAT CAAAAGGAAC TGGTGCTGGG GAGTTTCAGG GACAGTTGTT GGGAACTATT
    GCATTTATGG CCCCGGAGGT TCTGAGAGGT CAGCAGTATG GTAGGAGCTG TGATGTGTGG
    AGCGTTGGCT GTGTTGTCAT AGAAATGGCT TGTGCTAAAC CTCCCTGGAA TGCAGAGAAA
    CACTCCAATC ATCTTGCTCT GATCTTTAAG ATTGCTAGTG CAACTACTGC TCCATCAATC
    CCTTCACATC TGTCTCCTGG CTTGAGGGAT GTGACTCTTC GGTGTTTAGA ACTTCAACCT
    CAGGACAGAC CCCCATCGAG GGAGCTGCTG AAACACCCAG TCTTCCGTAC TACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133062.1
    CDS16..4215
    Translation

    Target ORF information:

    RefSeq Version XM_015277576.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGTGGG TTTTTAGATA CAGCCTAGAT GCAAACAAGG AGGTGAAAGT TTACAGCAGA 
    TCAGAGATCA ACTGGGGCAA ACTCATGCAG AGGCATCCTC CTCTGATTCC CCCCTGGAGC
    AACCAATTGT TGGTAAAAGA GAGGAAGAAT TGTCGTGACA TGGAGAATAA AGAAACCCTC
    AGGGGGTTGC AGAAGATGGA TGACCGCCCA GAAGAGCGCA TGATCAGGGA GAAACTCAAG
    GCAACGTGTA TGCCAGCTTG GAAGCATGAA TGGCTGGAAA GAAGAAGCAG GAGAGGCCCT
    GTGGTGGTGA AACCTATCCC TGTGAAGGGA GATGGATCTG AAATGAACAA GCTATCTCTA
    GAACCTCAAT CTGAAGGACA AAGCTCTGCT TCTTCACCTA CGCAGAAAGG AAGGCGTAGT
    CCTTCTCCTA GCAGTTCTTC TTCATCATCC TCTCGGACTG TTAAATCTGA GTCACCAGGT
    GTTAGAAGAA AAAGGGTATC TCCAGTACCT TTTCAGAGTG GACGAATAAC ACCACCTCGA
    AGAGCCCCAT CTCCAGATGG TTTCTCTCCA TACAGCCCTG AGGAAACAAG TCGTCGTGTC
    AACAAAGTTA TGCGAGCCAG GCTGTACCTG CTGCAGCAGA TAGGACCCAA CTCTTTCTTA
    ATTGGAGGAG ATAGCCCTGA TAATAAATAC AGAGTGTTTA TTGGACCTCA GACATGTAGC
    TGTGGCCGTG GAACATTTTG TATTCATCTG CTCTTTGTTA TGCTGCGAGT GTTCCAGCTT
    GAGCCTTCAG ACCCAATGCT GTGGAGAAAG ACACTGAAAA ACTTTGAGGT TGAGAGTTTG
    TTCCAGAAAT ATCACAGTAG GCGTAGCTCG AGGATCAAAG CTCCATCTCG TAACACCATC
    CAGAAGTTTG TCTCACGCAT GTCAAATTCT CATACATTAT CATCTAGTAC TTCTACATCT
    AGTTCAGAAA ACAGTATGAA AGATGAAGAG GAGCAGATGT GCCCCATTTG TTTGCTGGGC
    ATGCTGGATG AAGAGAGCCT GACTGTGTGT GAGGATGGCT GCAGGAACAA ATTACACCAC
    CACTGCATGT CAATATGGGC AGAAGAGTGT AGAAGAAACA GAGAGCCACT TATATGTCCT
    CTGTGTAGAT CTAAATGGAG ATCTCATGAT TTCTACAGTC ATGAATTGCC AAGCCCTGTG
    GATTCTTCTG TTCTCCATGT GGTTCAACAA CAAACTCAAC AGCAATCAAC AATTGGATCA
    CAGAGGAGAA CCCAGGATAG CAATTTCAAC CTTACTCATT ATGGGGTCCA GCAGATTCCT
    TCTGCCTATA AAGATTTAGC TGAGCCATGG ATTCAGGTCT TTGGAATGGA GTTAGTTGGC
    TGCTTGTTTT CTCGAAACTG GAATATACGA GAGATGGCAC TTAGACGTCT TTCCCATGAT
    GTTAGTGGTG CTTTATTACT GGCTAATGGT GAAAGCACTG GAAGTTCTGG AAATAGCAAT
    GGAAACAACA CGAGTGCTGG AGCTCTGGGA ATAGCGAGTG GGTCATCTCA GGCCACCGTC
    TCAGGAGATG TTGTGGTGGA ATCTTGCTGC AGTGTGCTAT CCATGGTCTG TGCTGACCCT
    GTCTACAAAG TGTATGTTGC TGCTTTAAAA ACCTTAAGAG CCATGCTGGT CTATACTCCT
    TGTCATAGTT TGGCAGAAAG GACTAAACTA CAGCGACTTC TGAAGCCAGT TGTAGAGACA
    ATATTAGTTA AATGTGCAGA TGCCAACAGT CGAACAAGTC AACTTTCAGT CTCAACGCTA
    TTGGAGATGT GTAAAGGCCA AGCAGGAGAG TTGGCAGTTG GGAGAGAAAT ACTTAAATCT
    GGGTCCATTG GTATTGGTGG TGTTGATTAT GTCTTAAATT GTATTCTTGC AACTGAAATT
    GAATCAAGCA ACTGGCAAGC GCTACTGGGG AGACTTTGTC TTATAGACAG ACTTCTGTTG
    GAATTTCCTG GTGAATTTTA TCCTCACATT GTCTGTGGAG ATGTTTTACA GGCTGATGCC
    ATAGTAGGCA GATATAAGAA ACTTCTCTCC CTTCTAAATT TCGCCTTGCT GTCAATTGAC
    AATTCCCACT CAATGGTTGG TAAACTATCA CGGAGAGTAT TTTTGAGTTC AGCAAGAATG
    GTTGCAAGAG TTCCTCACGT GTTTGTAAAG CTGTTAGAAA TGTTGAGTGT GACAAGCTCA
    ACTCATTACA CAAGGATGCG TCGACGTCTG ATGGCAATAG CAGATGAAAT GGAAGTTGCA
    GAAGCCATCC AGCTTGGCAT GGAAGAAATA CAGTCTGGGG AATGTCGACA TGAAGACTTT
    CTGCAGCTAC CCGTACCAGA TAATTCTCCA GAAGCTACAG AAAATAATAC TCCTAACAAT
    ACTGTCCAGT TATCAGGGAA AAATGGGAAA GGCTTAAGTG ACAAAAAACT GAGTGCTGGT
    CCAGAGGACA TTTCTGAGAC ACTGACTAGC CTAGTTGTGG GACATCCTGT TTCATCACTA
    ACCACTGAGC AACCAAAGCC AGCTGTTCAA ACAAAAGGAA AACCCTACAG CCAGTGTTTG
    AACTCTTCTC CCTCATCCAG TCATTCCCAG TCACTATTCC CAACACTTCT CTCTCCTTCA
    AACCCATCTG TATCAGCTGG CACTGTAACA GATGTCTCTA AACTCCGACC TCAGGGATTC
    ATTCCCTGCA AAATCCCTTC TCCTTCTCCT CAAACACAAC GGAAGCTTTC CCTCCAGTTT
    CAGAGAAGCT GCTCTGAAAA TAAAGAACCA GATAAGCTTT CTCCAGTTTT TACCCAAGCC
    AGGCCATTGC CATCTAGTCA CATACACAGG CCAAAGCCAT CACGACCCAC TCCAAATGAT
    ATGAACAAGC AGGGAGAAAC TTCAAAGAAC AGCATGACAC TTGACCTGAA TGATACTTCA
    CATTGTGATG GCAATGGTAG TAACAGTAGT GCAGTTATAC CAAGTGAAGA GACAGTGTTC
    ACACCAGTAG ATGAAAAATG TCGGCTGGAT GTTAATGCAG AGCTCAACTC CAGTATTGAG
    GACCTCCTTG AGGCCTCCAT GCCAACAAGT GATGGCACAG TTACTTTCAA GTCCGAAGTT
    GCAGTTCTTT CTCCCGAGAG GGCTGAAAAT GATGATACTT ACAAAGATGA TGTAAATCAT
    AATCAAAAAT GCAAGGAAAA GATGGAAGCT GAAGAAGAGG AAGCTTTAGC TATTGCTATG
    GCAATGTCTG CATCTCAAGA TGCCCTGCCA ATAATTCCCC AACTACAGGT CGAAAATGGT
    GAAGATATCA TAATTATTCA GCAAGATACA CCAGAAACTC TGCCTGGACA TACCAAAGCA
    AAGCATCATT ACAGGGAAGA TGCAGAATGG CTTAAAGGTC AGCAAATTGG TCTTGGAGCT
    TTCTCTTCCT GTTACCAAGC TCAAGATGTA GGAACAGGGA CATTAATGGC TGTAAAACAG
    GTGACATATG TCAGGAACAC ATCATCTGAG CAAGAAGAGG TAGTGGAAGC ACTGAGGGAG
    GAAATACGGA TGATGAGTCA TCTAAACCAT CCTAATATTA TTCGCATGTT GGGTGCTACA
    TGTGAGAAGA GCAACTACAA CCTCTTCATT GAATGGATGG CAGGGGGATC AGTTGCTCAT
    TTGTTGAGTA AATACGGAGC CTTCAAAGAA TCAGTTATTA TTAACTACAC AGAGCAACTG
    TTACGTGGCC TTTCTTACCT CCATGAGAAT CAGATAATTC ATAGAGATGT CAAAGGTGCC
    AATTTGCTAA TTGACAGCAC AGGTCATAGA TTAAGAATTG CTGATTTTGG AGCTGCAGCC
    AGGTTGGCAT CAAAAGGAAC TGGTGCTGGG GAGTTTCAGG GACAGTTGTT GGGAACTATT
    GCATTTATGG CCCCGGAGGT TCTGAGAGGT CAGCAGTATG GTAGGAGCTG TGATGTGTGG
    AGCGTTGGCT GTGTTGTCAT AGAAATGGCT TGTGCTAAAC CTCCCTGGAA TGCAGAGAAA
    CACTCCAATC ATCTTGCTCT GATCTTTAAG ATTGCTAGTG CAACTACTGC TCCATCAATC
    CCTTCACATC TGTCTCCTGG CTTGAGGGAT GTGACTCTTC GGTGTTTAGA ACTTCAACCT
    CAGGACAGAC CCCCATCGAG GGAGCTGCTG AAACACCCAG TCTTCCGTAC TACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47870
    Clone ID Related Accession (Same CDS sequence) XM_015277577.1 , XM_015277578.1 , XM_015277579.1
    Accession Version XM_015277577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133063.1
    CDS198..4238
    Translation

    Target ORF information:

    RefSeq Version XM_015277577.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47870
    Clone ID Related Accession (Same CDS sequence) XM_015277577.1 , XM_015277578.1 , XM_015277579.1
    Accession Version XM_015277578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133064.1
    CDS440..4480
    Translation

    Target ORF information:

    RefSeq Version XM_015277578.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47870
    Clone ID Related Accession (Same CDS sequence) XM_015277577.1 , XM_015277578.1 , XM_015277579.1
    Accession Version XM_015277579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133065.1
    CDS182..4222
    Translation

    Target ORF information:

    RefSeq Version XM_015277579.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACCCT CAGGGGGTTG CAGAAGATGG ATGACCGCCC AGAAGAGCGC 
    ATGATCAGGG AGAAACTCAA GGCAACGTGT ATGCCAGCTT GGAAGCATGA ATGGCTGGAA
    AGAAGAAGCA GGAGAGGCCC TGTGGTGGTG AAACCTATCC CTGTGAAGGG AGATGGATCT
    GAAATGAACA AGCTATCTCT AGAACCTCAA TCTGAAGGAC AAAGCTCTGC TTCTTCACCT
    ACGCAGAAAG GAAGGCGTAG TCCTTCTCCT AGCAGTTCTT CTTCATCATC CTCTCGGACT
    GTTAAATCTG AGTCACCAGG TGTTAGAAGA AAAAGGGTAT CTCCAGTACC TTTTCAGAGT
    GGACGAATAA CACCACCTCG AAGAGCCCCA TCTCCAGATG GTTTCTCTCC ATACAGCCCT
    GAGGAAACAA GTCGTCGTGT CAACAAAGTT ATGCGAGCCA GGCTGTACCT GCTGCAGCAG
    ATAGGACCCA ACTCTTTCTT AATTGGAGGA GATAGCCCTG ATAATAAATA CAGAGTGTTT
    ATTGGACCTC AGACATGTAG CTGTGGCCGT GGAACATTTT GTATTCATCT GCTCTTTGTT
    ATGCTGCGAG TGTTCCAGCT TGAGCCTTCA GACCCAATGC TGTGGAGAAA GACACTGAAA
    AACTTTGAGG TTGAGAGTTT GTTCCAGAAA TATCACAGTA GGCGTAGCTC GAGGATCAAA
    GCTCCATCTC GTAACACCAT CCAGAAGTTT GTCTCACGCA TGTCAAATTC TCATACATTA
    TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AAGATGAAGA GGAGCAGATG
    TGCCCCATTT GTTTGCTGGG CATGCTGGAT GAAGAGAGCC TGACTGTGTG TGAGGATGGC
    TGCAGGAACA AATTACACCA CCACTGCATG TCAATATGGG CAGAAGAGTG TAGAAGAAAC
    AGAGAGCCAC TTATATGTCC TCTGTGTAGA TCTAAATGGA GATCTCATGA TTTCTACAGT
    CATGAATTGC CAAGCCCTGT GGATTCTTCT GTTCTCCATG TGGTTCAACA ACAAACTCAA
    CAGCAATCAA CAATTGGATC ACAGAGGAGA ACCCAGGATA GCAATTTCAA CCTTACTCAT
    TATGGGGTCC AGCAGATTCC TTCTGCCTAT AAAGATTTAG CTGAGCCATG GATTCAGGTC
    TTTGGAATGG AGTTAGTTGG CTGCTTGTTT TCTCGAAACT GGAATATACG AGAGATGGCA
    CTTAGACGTC TTTCCCATGA TGTTAGTGGT GCTTTATTAC TGGCTAATGG TGAAAGCACT
    GGAAGTTCTG GAAATAGCAA TGGAAACAAC ACGAGTGCTG GAGCTCTGGG AATAGCGAGT
    GGGTCATCTC AGGCCACCGT CTCAGGAGAT GTTGTGGTGG AATCTTGCTG CAGTGTGCTA
    TCCATGGTCT GTGCTGACCC TGTCTACAAA GTGTATGTTG CTGCTTTAAA AACCTTAAGA
    GCCATGCTGG TCTATACTCC TTGTCATAGT TTGGCAGAAA GGACTAAACT ACAGCGACTT
    CTGAAGCCAG TTGTAGAGAC AATATTAGTT AAATGTGCAG ATGCCAACAG TCGAACAAGT
    CAACTTTCAG TCTCAACGCT ATTGGAGATG TGTAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGGAGAGAAA TACTTAAATC TGGGTCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT
    TGTATTCTTG CAACTGAAAT TGAATCAAGC AACTGGCAAG CGCTACTGGG GAGACTTTGT
    CTTATAGACA GACTTCTGTT GGAATTTCCT GGTGAATTTT ATCCTCACAT TGTCTGTGGA
    GATGTTTTAC AGGCTGATGC CATAGTAGGC AGATATAAGA AACTTCTCTC CCTTCTAAAT
    TTCGCCTTGC TGTCAATTGA CAATTCCCAC TCAATGGTTG GTAAACTATC ACGGAGAGTA
    TTTTTGAGTT CAGCAAGAAT GGTTGCAAGA GTTCCTCACG TGTTTGTAAA GCTGTTAGAA
    ATGTTGAGTG TGACAAGCTC AACTCATTAC ACAAGGATGC GTCGACGTCT GATGGCAATA
    GCAGATGAAA TGGAAGTTGC AGAAGCCATC CAGCTTGGCA TGGAAGAAAT ACAGTCTGGG
    GAATGTCGAC ATGAAGACTT TCTGCAGCTA CCCGTACCAG ATAATTCTCC AGAAGCTACA
    GAAAATAATA CTCCTAACAA TACTGTCCAG TTATCAGGGA AAAATGGGAA AGGCTTAAGT
    GACAAAAAAC TGAGTGCTGG TCCAGAGGAC ATTTCTGAGA CACTGACTAG CCTAGTTGTG
    GGACATCCTG TTTCATCACT AACCACTGAG CAACCAAAGC CAGCTGTTCA AACAAAAGGA
    AAACCCTACA GCCAGTGTTT GAACTCTTCT CCCTCATCCA GTCATTCCCA GTCACTATTC
    CCAACACTTC TCTCTCCTTC AAACCCATCT GTATCAGCTG GCACTGTAAC AGATGTCTCT
    AAACTCCGAC CTCAGGGATT CATTCCCTGC AAAATCCCTT CTCCTTCTCC TCAAACACAA
    CGGAAGCTTT CCCTCCAGTT TCAGAGAAGC TGCTCTGAAA ATAAAGAACC AGATAAGCTT
    TCTCCAGTTT TTACCCAAGC CAGGCCATTG CCATCTAGTC ACATACACAG GCCAAAGCCA
    TCACGACCCA CTCCAAATGA TATGAACAAG CAGGGAGAAA CTTCAAAGAA CAGCATGACA
    CTTGACCTGA ATGATACTTC ACATTGTGAT GGCAATGGTA GTAACAGTAG TGCAGTTATA
    CCAAGTGAAG AGACAGTGTT CACACCAGTA GATGAAAAAT GTCGGCTGGA TGTTAATGCA
    GAGCTCAACT CCAGTATTGA GGACCTCCTT GAGGCCTCCA TGCCAACAAG TGATGGCACA
    GTTACTTTCA AGTCCGAAGT TGCAGTTCTT TCTCCCGAGA GGGCTGAAAA TGATGATACT
    TACAAAGATG ATGTAAATCA TAATCAAAAA TGCAAGGAAA AGATGGAAGC TGAAGAAGAG
    GAAGCTTTAG CTATTGCTAT GGCAATGTCT GCATCTCAAG ATGCCCTGCC AATAATTCCC
    CAACTACAGG TCGAAAATGG TGAAGATATC ATAATTATTC AGCAAGATAC ACCAGAAACT
    CTGCCTGGAC ATACCAAAGC AAAGCATCAT TACAGGGAAG ATGCAGAATG GCTTAAAGGT
    CAGCAAATTG GTCTTGGAGC TTTCTCTTCC TGTTACCAAG CTCAAGATGT AGGAACAGGG
    ACATTAATGG CTGTAAAACA GGTGACATAT GTCAGGAACA CATCATCTGA GCAAGAAGAG
    GTAGTGGAAG CACTGAGGGA GGAAATACGG ATGATGAGTC ATCTAAACCA TCCTAATATT
    ATTCGCATGT TGGGTGCTAC ATGTGAGAAG AGCAACTACA ACCTCTTCAT TGAATGGATG
    GCAGGGGGAT CAGTTGCTCA TTTGTTGAGT AAATACGGAG CCTTCAAAGA ATCAGTTATT
    ATTAACTACA CAGAGCAACT GTTACGTGGC CTTTCTTACC TCCATGAGAA TCAGATAATT
    CATAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCATAG ATTAAGAATT
    GCTGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCTGG GGAGTTTCAG
    GGACAGTTGT TGGGAACTAT TGCATTTATG GCCCCGGAGG TTCTGAGAGG TCAGCAGTAT
    GGTAGGAGCT GTGATGTGTG GAGCGTTGGC TGTGTTGTCA TAGAAATGGC TTGTGCTAAA
    CCTCCCTGGA ATGCAGAGAA ACACTCCAAT CATCTTGCTC TGATCTTTAA GATTGCTAGT
    GCAACTACTG CTCCATCAAT CCCTTCACAT CTGTCTCCTG GCTTGAGGGA TGTGACTCTT
    CGGTGTTTAG AACTTCAACC TCAGGACAGA CCCCCATCGA GGGAGCTGCT GAAACACCCA
    GTCTTCCGTA CTACATGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47868
    Clone ID Related Accession (Same CDS sequence) XM_015277575.1 , XM_015277575.2
    Accession Version XM_015277575.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
    AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC
    CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG
    TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC
    CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG
    GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG
    CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC
    GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG
    CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC
    GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG
    AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG
    TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG
    TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG
    GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT
    CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT
    CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA
    AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC
    CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA
    GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA
    GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC
    CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT
    TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG
    AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG
    TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA
    GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG
    GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC
    ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT
    AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT
    TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG
    AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC
    TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG
    TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT
    GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC
    AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA
    GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC
    AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT
    AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA
    GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG
    ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC
    ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA
    AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT
    CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA
    GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC
    CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA
    AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT
    TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC
    ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG
    CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC
    CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG
    GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT
    GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT
    TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA
    TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC
    TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA
    AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA
    TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC
    AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT
    GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA
    GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC
    CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT
    CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA
    AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG
    TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT
    ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT
    CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT
    TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA
    TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA
    CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG
    AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG
    AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT
    GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG
    CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG
    GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT
    ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT
    GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC
    AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA
    CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC
    AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133061.1
    CDS32..4705
    Translation

    Target ORF information:

    RefSeq Version XM_015277575.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
    AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC
    CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG
    TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC
    CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG
    GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG
    CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC
    GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG
    CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC
    GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG
    AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG
    TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG
    TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG
    GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT
    CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT
    CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA
    AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC
    CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA
    GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA
    GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC
    CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT
    TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG
    AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG
    TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA
    GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG
    GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC
    ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT
    AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT
    TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG
    AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC
    TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG
    TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT
    GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC
    AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA
    GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC
    AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT
    AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA
    GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG
    ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC
    ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA
    AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT
    CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA
    GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC
    CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA
    AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT
    TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC
    ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG
    CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC
    CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG
    GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT
    GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT
    TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA
    TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC
    TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA
    AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA
    TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC
    AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT
    GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA
    GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC
    CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT
    CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA
    AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG
    TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT
    ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT
    CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT
    TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA
    TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA
    CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG
    AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG
    AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT
    GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG
    CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG
    GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT
    ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT
    GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC
    AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA
    CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC
    AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47868
    Clone ID Related Accession (Same CDS sequence) XM_015277575.1 , XM_015277575.2
    Accession Version XM_015277575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
    AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC
    CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG
    TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC
    CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG
    GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG
    CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC
    GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG
    CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC
    GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG
    AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG
    TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG
    TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG
    GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT
    CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT
    CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA
    AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC
    CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA
    GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA
    GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC
    CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT
    TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG
    AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG
    TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA
    GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG
    GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC
    ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT
    AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT
    TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG
    AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC
    TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG
    TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT
    GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC
    AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA
    GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC
    AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT
    AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA
    GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG
    ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC
    ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA
    AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT
    CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA
    GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC
    CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA
    AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT
    TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC
    ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG
    CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC
    CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG
    GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT
    GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT
    TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA
    TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC
    TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA
    AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA
    TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC
    AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT
    GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA
    GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC
    CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT
    CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA
    AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG
    TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT
    ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT
    CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT
    TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA
    TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA
    CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG
    AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG
    AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT
    GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG
    CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG
    GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT
    ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT
    GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC
    AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA
    CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC
    AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133061.1
    CDS394..5067
    Translation

    Target ORF information:

    RefSeq Version XM_015277575.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCGCTC GCCGCCCGAC CCCCTCCCTA CGCCGTTTGC GCAGGCAGCG CCGTTTCCGC 
    AGCAGGCGGG AGCGCCGGCT CCCTCCTCCT TCTCTCCCTC CCTCCGCCCC CGCCCGAGTC
    CCGGGAGCCC GATGTGACCC GCCAGAGAAA ATGGCGGCGG CGGCGGGGAA TCGCACCTCG
    TCGTCGGGAT TATCCTCGGG CGGCGGCGCG GAAGGGGCCG CCGCCGCCGC ATGCGGGAGC
    CCGAAGGGCG GCGCGGGCGA GGGCAGCGGT GCGGGGAGCG GCGGGCTGCT GCGGGAGGCG
    GGCGGCGGCG GCGGCCGGGA GCAGCGCTCC GACTGGCGGC GGAAGCAGCT GCGCAAAGTG
    CGGAGCGTGG AGCTGGACCG GCTGCCCGAG GCGCCCCTCT TACTCGCAGC CGCCCCTGCC
    GCCGCCTCCT CCTCGTCGTC CTCCTCCCCT TCGCCGGAGC CCCCTGAGGC GCTGCTGTTG
    CACTACCTGC CCGGGCCGCG CTCCGGCCCC GCCAGCCCCG CCGTGTCCCC CGGCCGCGGC
    GCCGAGCTCC TGGAGCTGCC GGCGGGCAGC CCCGCGGCCG CCGACCCCGC CGCCGAGAGG
    AGGATGGAGC CGTCCCCCGC CGCGAGTCGT GACATGGAGA ATAAAGAAAC CCTCAGGGGG
    TTGCAGAAGA TGGATGACCG CCCAGAAGAG CGCATGATCA GGGAGAAACT CAAGGCAACG
    TGTATGCCAG CTTGGAAGCA TGAATGGCTG GAAAGAAGAA GCAGGAGAGG CCCTGTGGTG
    GTGAAACCTA TCCCTGTGAA GGGAGATGGA TCTGAAATGA ACAAGCTATC TCTAGAACCT
    CAATCTGAAG GACAAAGCTC TGCTTCTTCA CCTACGCAGA AAGGAAGGCG TAGTCCTTCT
    CCTAGCAGTT CTTCTTCATC ATCCTCTCGG ACTGTTAAAT CTGAGTCACC AGGTGTTAGA
    AGAAAAAGGG TATCTCCAGT ACCTTTTCAG AGTGGACGAA TAACACCACC TCGAAGAGCC
    CCATCTCCAG ATGGTTTCTC TCCATACAGC CCTGAGGAAA CAAGTCGTCG TGTCAACAAA
    GTTATGCGAG CCAGGCTGTA CCTGCTGCAG CAGATAGGAC CCAACTCTTT CTTAATTGGA
    GGAGATAGCC CTGATAATAA ATACAGAGTG TTTATTGGAC CTCAGACATG TAGCTGTGGC
    CGTGGAACAT TTTGTATTCA TCTGCTCTTT GTTATGCTGC GAGTGTTCCA GCTTGAGCCT
    TCAGACCCAA TGCTGTGGAG AAAGACACTG AAAAACTTTG AGGTTGAGAG TTTGTTCCAG
    AAATATCACA GTAGGCGTAG CTCGAGGATC AAAGCTCCAT CTCGTAACAC CATCCAGAAG
    TTTGTCTCAC GCATGTCAAA TTCTCATACA TTATCATCTA GTACTTCTAC ATCTAGTTCA
    GAAAACAGTA TGAAAGATGA AGAGGAGCAG ATGTGCCCCA TTTGTTTGCT GGGCATGCTG
    GATGAAGAGA GCCTGACTGT GTGTGAGGAT GGCTGCAGGA ACAAATTACA CCACCACTGC
    ATGTCAATAT GGGCAGAAGA GTGTAGAAGA AACAGAGAGC CACTTATATG TCCTCTGTGT
    AGATCTAAAT GGAGATCTCA TGATTTCTAC AGTCATGAAT TGCCAAGCCC TGTGGATTCT
    TCTGTTCTCC ATGTGGTTCA ACAACAAACT CAACAGCAAT CAACAATTGG ATCACAGAGG
    AGAACCCAGG ATAGCAATTT CAACCTTACT CATTATGGGG TCCAGCAGAT TCCTTCTGCC
    TATAAAGATT TAGCTGAGCC ATGGATTCAG GTCTTTGGAA TGGAGTTAGT TGGCTGCTTG
    TTTTCTCGAA ACTGGAATAT ACGAGAGATG GCACTTAGAC GTCTTTCCCA TGATGTTAGT
    GGTGCTTTAT TACTGGCTAA TGGTGAAAGC ACTGGAAGTT CTGGAAATAG CAATGGAAAC
    AACACGAGTG CTGGAGCTCT GGGAATAGCG AGTGGGTCAT CTCAGGCCAC CGTCTCAGGA
    GATGTTGTGG TGGAATCTTG CTGCAGTGTG CTATCCATGG TCTGTGCTGA CCCTGTCTAC
    AAAGTGTATG TTGCTGCTTT AAAAACCTTA AGAGCCATGC TGGTCTATAC TCCTTGTCAT
    AGTTTGGCAG AAAGGACTAA ACTACAGCGA CTTCTGAAGC CAGTTGTAGA GACAATATTA
    GTTAAATGTG CAGATGCCAA CAGTCGAACA AGTCAACTTT CAGTCTCAAC GCTATTGGAG
    ATGTGTAAAG GCCAAGCAGG AGAGTTGGCA GTTGGGAGAG AAATACTTAA ATCTGGGTCC
    ATTGGTATTG GTGGTGTTGA TTATGTCTTA AATTGTATTC TTGCAACTGA AATTGAATCA
    AGCAACTGGC AAGCGCTACT GGGGAGACTT TGTCTTATAG ACAGACTTCT GTTGGAATTT
    CCTGGTGAAT TTTATCCTCA CATTGTCTGT GGAGATGTTT TACAGGCTGA TGCCATAGTA
    GGCAGATATA AGAAACTTCT CTCCCTTCTA AATTTCGCCT TGCTGTCAAT TGACAATTCC
    CACTCAATGG TTGGTAAACT ATCACGGAGA GTATTTTTGA GTTCAGCAAG AATGGTTGCA
    AGAGTTCCTC ACGTGTTTGT AAAGCTGTTA GAAATGTTGA GTGTGACAAG CTCAACTCAT
    TACACAAGGA TGCGTCGACG TCTGATGGCA ATAGCAGATG AAATGGAAGT TGCAGAAGCC
    ATCCAGCTTG GCATGGAAGA AATACAGTCT GGGGAATGTC GACATGAAGA CTTTCTGCAG
    CTACCCGTAC CAGATAATTC TCCAGAAGCT ACAGAAAATA ATACTCCTAA CAATACTGTC
    CAGTTATCAG GGAAAAATGG GAAAGGCTTA AGTGACAAAA AACTGAGTGC TGGTCCAGAG
    GACATTTCTG AGACACTGAC TAGCCTAGTT GTGGGACATC CTGTTTCATC ACTAACCACT
    GAGCAACCAA AGCCAGCTGT TCAAACAAAA GGAAAACCCT ACAGCCAGTG TTTGAACTCT
    TCTCCCTCAT CCAGTCATTC CCAGTCACTA TTCCCAACAC TTCTCTCTCC TTCAAACCCA
    TCTGTATCAG CTGGCACTGT AACAGATGTC TCTAAACTCC GACCTCAGGG ATTCATTCCC
    TGCAAAATCC CTTCTCCTTC TCCTCAAACA CAACGGAAGC TTTCCCTCCA GTTTCAGAGA
    AGCTGCTCTG AAAATAAAGA ACCAGATAAG CTTTCTCCAG TTTTTACCCA AGCCAGGCCA
    TTGCCATCTA GTCACATACA CAGGCCAAAG CCATCACGAC CCACTCCAAA TGATATGAAC
    AAGCAGGGAG AAACTTCAAA GAACAGCATG ACACTTGACC TGAATGATAC TTCACATTGT
    GATGGCAATG GTAGTAACAG TAGTGCAGTT ATACCAAGTG AAGAGACAGT GTTCACACCA
    GTAGATGAAA AATGTCGGCT GGATGTTAAT GCAGAGCTCA ACTCCAGTAT TGAGGACCTC
    CTTGAGGCCT CCATGCCAAC AAGTGATGGC ACAGTTACTT TCAAGTCCGA AGTTGCAGTT
    CTTTCTCCCG AGAGGGCTGA AAATGATGAT ACTTACAAAG ATGATGTAAA TCATAATCAA
    AAATGCAAGG AAAAGATGGA AGCTGAAGAA GAGGAAGCTT TAGCTATTGC TATGGCAATG
    TCTGCATCTC AAGATGCCCT GCCAATAATT CCCCAACTAC AGGTCGAAAA TGGTGAAGAT
    ATCATAATTA TTCAGCAAGA TACACCAGAA ACTCTGCCTG GACATACCAA AGCAAAGCAT
    CATTACAGGG AAGATGCAGA ATGGCTTAAA GGTCAGCAAA TTGGTCTTGG AGCTTTCTCT
    TCCTGTTACC AAGCTCAAGA TGTAGGAACA GGGACATTAA TGGCTGTAAA ACAGGTGACA
    TATGTCAGGA ACACATCATC TGAGCAAGAA GAGGTAGTGG AAGCACTGAG GGAGGAAATA
    CGGATGATGA GTCATCTAAA CCATCCTAAT ATTATTCGCA TGTTGGGTGC TACATGTGAG
    AAGAGCAACT ACAACCTCTT CATTGAATGG ATGGCAGGGG GATCAGTTGC TCATTTGTTG
    AGTAAATACG GAGCCTTCAA AGAATCAGTT ATTATTAACT ACACAGAGCA ACTGTTACGT
    GGCCTTTCTT ACCTCCATGA GAATCAGATA ATTCATAGAG ATGTCAAAGG TGCCAATTTG
    CTAATTGACA GCACAGGTCA TAGATTAAGA ATTGCTGATT TTGGAGCTGC AGCCAGGTTG
    GCATCAAAAG GAACTGGTGC TGGGGAGTTT CAGGGACAGT TGTTGGGAAC TATTGCATTT
    ATGGCCCCGG AGGTTCTGAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGCGTT
    GGCTGTGTTG TCATAGAAAT GGCTTGTGCT AAACCTCCCT GGAATGCAGA GAAACACTCC
    AATCATCTTG CTCTGATCTT TAAGATTGCT AGTGCAACTA CTGCTCCATC AATCCCTTCA
    CATCTGTCTC CTGGCTTGAG GGATGTGACT CTTCGGTGTT TAGAACTTCA ACCTCAGGAC
    AGACCCCCAT CGAGGGAGCT GCTGAAACAC CCAGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.