Map3k1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k1
Entrez Gene ID	26401
Full Name	mitogen-activated protein kinase kinase kinase 1
Synonyms	MAPKKK1,MEKK1,Mekk
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 1 Names mitogen-activated protein kinase kinase kinase 1 MAPK/ERK kinase kinase 1 MEK kinase 1 MEKK 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 D2.2\|13 63.36 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315517.2	XP_017171006.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
NM_011945.2	NP_036075.2	mitogen-activated protein kinase kinase kinase 1
XM_017315517.1	XP_017171006.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
NM_011945.3	NP_036075.2	mitogen-activated protein kinase kinase kinase 1
XM_017315516.1	XP_017171005.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_017315516.2	XP_017171005.1	mitogen-activated protein kinase kinase kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K1	NP_005912.1
Rattus norvegicus (Norway rat)	LOC100912399	XP_006231988.1
	MAP3K1	XP_002804414.1
Canis lupus familiaris (dog)	MAP3K1	XP_535240.4
Xenopus tropicalis (tropical clawed frog)	map3k1	XP_002940279.2
Rattus norvegicus (Norway rat)	Map3k1	NP_446339.1
Pan troglodytes (chimpanzee)	MAP3K1	XP_003310777.1
Bos taurus (cattle)	MAP3K1	NP_001192835.1
Mus musculus (house mouse)	Map3k1	NP_036075.2
Gallus gallus (chicken)	MAP3K1	XP_424734.3
Danio rerio (zebrafish)	map3k1	XP_002663531.2

Gene Ontology
Bibliography

Related articles in PubMed

MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility.
Hirata H, Ku WC, Yip AK, Ursekar CP, Kawauchi K, Roy A, Guo AK, Vedula SR, Harada I, Chiam KH, Ishihama Y, Lim CT, Sawada Y, Sokabe M
Journal of cell science129(19)3574-3582(2016 10)

T-Cell-Specific Deletion of Map3k1 Reveals the Critical Role for Mekk1 and Jnks in Cdkn1b-Dependent Proliferative Expansion.
Suddason T, Anwar S, Charlaftis N, Gallagher E
Cell reports14(3)449-457(2016 Jan)

MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells.
Yousaf R, Meng Q, Hufnagel RB, Xia Y, Puligilla C, Ahmed ZM, Riazuddin S
Disease models & mechanisms8(12)1543-53(2015 Dec)

The goya mouse mutant reveals distinct newly identified roles for MAP3K1 in the development and survival of cochlear sensory hair cells.
Parker A, Cross SH, Jackson IJ, Hardisty-Hughes R, Morse S, Nicholson G, Coghill E, Bowl MR, Brown SD
Disease models & mechanisms8(12)1555-68(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal the importance of the MEKK1-calponin-3 signaling pathway to cell contractility.
Title: MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility.

Map3k1 regulates iNKT cell proliferative expansion in response to glycolipid antigen
Title: T-Cell-Specific Deletion of Map3k1 Reveals the Critical Role for Mekk1 and Jnks in Cdkn1b-Dependent Proliferative Expansion.

These data highlight the crucial role of MAP3K1 in the development and function of the mouse inner ear and hearing.
Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells.

goya and kinase-deficient Map3k1 homozygotes initially develop supernumerary cochlear outer hair cells (OHCs) that subsequently degenerate.
Title: The goya mouse mutant reveals distinct newly identified roles for MAP3K1 in the development and survival of cochlear sensory hair cells.

Dioxin exposure markedly inhibited c-Jun phosphorylation in Map3k1-deficient embryonic eyelid epithelium, suggesting that dioxin-induced AHR pathways can synergize with gene mutations to inhibit MAP3K1 signaling.
Title: Gene-Environment Interactions Target Mitogen-activated Protein 3 Kinase 1 (MAP3K1) Signaling in Eyelid Morphogenesis.

The following Map3k1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu49052	XM_017315516.2 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69202	XM_017315517.2 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu14906	NM_011945.3 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu69202	XM_017315517.1	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu14906	NM_011945.2	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu49052	XM_017315516.1	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k1 ( XM_017315517.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171006.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69202
Clone ID Related Accession (Same CDS sequence)	XM_017315517.2 , XM_017315517.1
Accession Version	XM_017315517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG 
AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT 
GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA 
GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG 
CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG 
ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC 
CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC 
GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA 
TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG 
TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT 
CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT 
GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC 
CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT 
CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG 
GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG 
GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC 
ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC 
TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC 
TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG 
GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA 
GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC 
ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA 
ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT 
GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC 
AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA 
CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG 
CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC 
TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT 
GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG 
GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC 
AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC 
CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC 
ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA 
CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT 
TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT 
GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA 
TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA 
GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA 
CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA 
AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC 
GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG 
GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA 
GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT 
CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC 
AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG 
TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC 
ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC 
AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT 
TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC 
AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG 
ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC 
AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA 
TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT 
TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT 
GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA 
AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG 
CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC 
GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT 
CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG 
TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT 
CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171006.1
CDS	3400..7407
Translation	MENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017315517.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315517.1

ORF Insert Sequence:

ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG 
AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT 
GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA 
GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG 
CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG 
ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC 
CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC 
GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA 
TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG 
TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT 
CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT 
GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC 
CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT 
CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG 
GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG 
GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC 
ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC 
TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC 
TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG 
GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA 
GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC 
ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA 
ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT 
GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC 
AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA 
CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG 
CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC 
TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT 
GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG 
GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC 
AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC 
CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC 
ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA 
CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT 
TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT 
GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA 
TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA 
GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA 
CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA 
AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC 
GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG 
GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA 
GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT 
CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC 
AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG 
TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC 
ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC 
AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT 
TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC 
AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG 
ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC 
AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA 
TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT 
TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT 
GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA 
AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG 
CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC 
GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT 
CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG 
TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT 
CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k1 ( XM_017315516.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171005.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu49052
Clone ID Related Accession (Same CDS sequence)	XM_017315516.1 , XM_017315516.2
Accession Version	XM_017315516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT 
ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG 
AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA 
GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT 
GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA 
GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG 
GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA 
GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT 
CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA 
TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC 
ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC 
AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA 
CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC 
AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG 
GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC 
ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT 
AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC 
TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA 
AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT 
TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA 
TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT 
GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC 
AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG 
GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT 
GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA 
AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA 
GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC 
CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT 
GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA 
GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC 
TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG 
AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT 
GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT 
GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG 
CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT 
GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT 
CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT 
GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA 
GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA 
CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA 
CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG 
GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA 
CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT 
CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG 
GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT 
GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC 
AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG 
CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC 
ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC 
AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG 
GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG 
CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT 
CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG 
CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT 
TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG 
GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC 
CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG 
GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT 
AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC 
AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA 
GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA 
CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT 
AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA 
CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA 
ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC 
TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC 
TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171005.1
CDS	16..4053
Translation	MLLPPEDSREMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017315516.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315516.2

ORF Insert Sequence:

ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT 
ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG 
AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA 
GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT 
GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA 
GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG 
GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA 
GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT 
CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA 
TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC 
ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC 
AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA 
CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC 
AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG 
GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC 
ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT 
AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC 
TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA 
AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT 
TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA 
TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT 
GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC 
AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG 
GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT 
GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA 
AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA 
GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC 
CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT 
GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA 
GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC 
TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG 
AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT 
GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT 
GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG 
CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT 
GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT 
CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT 
GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA 
GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA 
CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA 
CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG 
GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA 
CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT 
CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG 
GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT 
GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC 
AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG 
CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC 
ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC 
AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG 
GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG 
CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT 
CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG 
CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT 
TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG 
GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC 
CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG 
GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT 
AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC 
AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA 
GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA 
CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT 
AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA 
CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA 
ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC 
TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC 
TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k1 ( XM_017315517.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171006.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

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CloneID	OMu69202
Clone ID Related Accession (Same CDS sequence)	XM_017315517.2 , XM_017315517.1
Accession Version	XM_017315517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG 
AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT 
GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA 
GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG 
CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG 
ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC 
CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC 
GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA 
TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG 
TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT 
CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT 
GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC 
CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT 
CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG 
GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG 
GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC 
ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC 
TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC 
TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG 
GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA 
GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC 
ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA 
ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT 
GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC 
AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA 
CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG 
CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC 
TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT 
GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG 
GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC 
AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC 
CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC 
ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA 
CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT 
TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT 
GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA 
TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA 
GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA 
CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA 
AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC 
GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG 
GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA 
GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT 
CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC 
AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG 
TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC 
ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC 
AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT 
TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC 
AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG 
ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC 
AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA 
TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT 
TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT 
GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA 
AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG 
CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC 
GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT 
CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG 
TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT 
CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171006.1
CDS	11916..15923
Translation	MENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017315517.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315517.2

ORF Insert Sequence:

ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG 
AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT 
GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA 
GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG 
CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG 
ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC 
CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC 
GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA 
TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG 
TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT 
CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT 
GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC 
CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT 
CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG 
GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG 
GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC 
ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC 
TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC 
TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG 
GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA 
GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC 
ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA 
ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT 
GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC 
AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA 
CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG 
CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC 
TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT 
GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG 
GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC 
AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC 
CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC 
ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA 
CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT 
TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT 
GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA 
TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA 
GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA 
CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA 
AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC 
GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG 
GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA 
GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT 
CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC 
AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG 
TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC 
ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC 
AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT 
TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC 
AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG 
ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC 
AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA 
TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT 
TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT 
GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA 
AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG 
CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC 
GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT 
CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG 
TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT 
CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k1 ( XM_017315516.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171005.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

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CloneID	OMu49052
Clone ID Related Accession (Same CDS sequence)	XM_017315516.1 , XM_017315516.2
Accession Version	XM_017315516.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT 
ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG 
AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA 
GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT 
GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA 
GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG 
GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA 
GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT 
CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA 
TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC 
ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC 
AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA 
CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC 
AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG 
GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC 
ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT 
AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC 
TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA 
AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT 
TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA 
TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT 
GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC 
AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG 
GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT 
GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA 
AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA 
GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC 
CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT 
GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA 
GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC 
TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG 
AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT 
GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT 
GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG 
CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT 
GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT 
CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT 
GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA 
GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA 
CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA 
CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG 
GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA 
CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT 
CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG 
GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT 
GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC 
AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG 
CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC 
ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC 
AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG 
GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG 
CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT 
CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG 
CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT 
TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG 
GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC 
CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG 
GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT 
AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC 
AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA 
GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA 
CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT 
AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA 
CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA 
ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC 
TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC 
TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171005.1
CDS	16..4053
Translation	MLLPPEDSREMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017315516.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315516.1

ORF Insert Sequence:

ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT 
ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG 
AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA 
GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT 
GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA 
GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG 
GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA 
GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT 
CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA 
TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC 
ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC 
AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA 
CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC 
AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG 
GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC 
ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT 
AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC 
TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA 
AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT 
TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA 
TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT 
GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC 
AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG 
GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT 
GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA 
AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA 
GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC 
CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT 
GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA 
GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC 
TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG 
AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT 
GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT 
GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG 
CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT 
GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT 
CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT 
GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA 
GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA 
CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA 
CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG 
GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA 
CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT 
CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG 
GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT 
GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC 
AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG 
CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC 
ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC 
AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG 
GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG 
CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT 
CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG 
CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT 
TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG 
GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC 
CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG 
GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT 
AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC 
AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA 
GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA 
CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT 
AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA 
CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA 
ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC 
TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC 
TTCCGTACCA CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k1 ( NM_011945.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036075.2 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

Price & Availability↑

CloneID	OMu14906
Clone ID Related Accession (Same CDS sequence)	NM_011945.2 , NM_011945.3
Accession Version	NM_011945.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC118704.17. On May 25, 2006 this sequence version replaced NM_011945.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC 
GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC 
TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG 
CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC 
GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA 
GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC 
CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG 
AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC 
CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG 
AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG 
AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC 
AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA 
TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA 
CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC 
CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC 
TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG 
AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG 
TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT 
GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG 
AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC 
ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC 
TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC 
AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT 
TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA 
TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG 
CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT 
GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT 
GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT 
AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA 
AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG 
CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT 
GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC 
ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA 
GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT 
ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT 
AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC 
CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG 
CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT 
GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC 
ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC 
AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT 
GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA 
ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA 
CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA 
GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT 
GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG 
CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT 
CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA 
GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT 
CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA 
GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG 
CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT 
AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC 
AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG 
TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC 
ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA 
AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA 
AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG 
GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT 
CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA 
GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA 
GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC 
ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT 
CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC 
AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA 
GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT 
CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC 
ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA 
ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG 
GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT 
ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC 
TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG 
GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC 
AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036075.2
CDS	15..4496
Translation	MAAAAGDRASSSGFPGAAAASPEAGGGGGGGGALQGSGAPAAGAAGLLREPGSAGRERADWRRRQLRKVRSVELDQLPEQPLFLAAASPPCPSTSPSPEPADAAAGASRFQPAAGPPPPGAASRCGSHSAELAAARDSGARSPAGAEPPSAAAPSGREMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	NM_011945.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011945.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC 
GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC 
TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG 
CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC 
GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA 
GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC 
CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG 
AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC 
CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG 
AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG 
AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC 
AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA 
TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA 
CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC 
CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC 
TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG 
AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG 
TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT 
GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG 
AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC 
ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC 
TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC 
AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT 
TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA 
TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG 
CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT 
GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT 
GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT 
AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA 
AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG 
CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT 
GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC 
ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA 
GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT 
ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT 
AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC 
CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG 
CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT 
GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC 
ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC 
AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT 
GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA 
ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA 
CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA 
GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT 
GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG 
CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT 
CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA 
GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT 
CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA 
GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG 
CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT 
AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC 
AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG 
TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC 
ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA 
AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA 
AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG 
GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT 
CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA 
GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA 
GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC 
ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT 
CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC 
AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA 
GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT 
CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC 
ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA 
ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG 
GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT 
ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC 
TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG 
GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC 
AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k1 ( NM_011945.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036075.2 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

Price & Availability↑

CloneID	OMu14906
Clone ID Related Accession (Same CDS sequence)	NM_011945.2 , NM_011945.3
Accession Version	NM_011945.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118704.17. On Apr 13, 2018 this sequence version replaced NM_011945.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC 
GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC 
TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG 
CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC 
GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA 
GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC 
CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG 
AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC 
CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG 
AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG 
AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC 
AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA 
TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA 
CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC 
CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC 
TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG 
AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG 
TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT 
GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG 
AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC 
ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC 
TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC 
AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT 
TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA 
TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG 
CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT 
GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT 
GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT 
AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA 
AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG 
CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT 
GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC 
ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA 
GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT 
ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT 
AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC 
CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG 
CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT 
GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC 
ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC 
AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT 
GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA 
ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA 
CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA 
GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT 
GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG 
CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT 
CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA 
GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT 
CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA 
GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG 
CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT 
AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC 
AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG 
TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC 
ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA 
AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA 
AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG 
GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT 
CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA 
GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA 
GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC 
ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT 
CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC 
AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA 
GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT 
CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC 
ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA 
ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG 
GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT 
ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC 
TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG 
GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC 
AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036075.2
CDS	25..4506
Translation	MAAAAGDRASSSGFPGAAAASPEAGGGGGGGGALQGSGAPAAGAAGLLREPGSAGRERADWRRRQLRKVRSVELDQLPEQPLFLAAASPPCPSTSPSPEPADAAAGASRFQPAAGPPPPGAASRCGSHSAELAAARDSGARSPAGAEPPSAAAPSGREMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEMNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPTSLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQVLAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSEETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	NM_011945.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011945.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC 
GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC 
TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG 
CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC 
GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA 
GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC 
CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG 
AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC 
CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG 
AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG 
AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC 
AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA 
TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA 
CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC 
CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC 
TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG 
AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG 
TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT 
GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG 
AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC 
ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC 
TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC 
AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT 
TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA 
TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG 
CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT 
GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT 
GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT 
AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA 
AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG 
CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT 
GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC 
ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA 
GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT 
ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT 
AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC 
CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG 
CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT 
GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC 
ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC 
AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT 
GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA 
ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA 
CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA 
GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT 
GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG 
CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT 
CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA 
GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT 
CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA 
GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG 
CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT 
AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC 
AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG 
TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC 
ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA 
AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA 
AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG 
GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT 
CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA 
GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA 
GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC 
ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT 
CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC 
AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA 
GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT 
CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC 
ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA 
ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG 
GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT 
ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC 
TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG 
GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC 
AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility.
Journal of cell science129(19)3574-3582(2016 10)
Hirata H,Ku WC,Yip AK,Ursekar CP,Kawauchi K,Roy A,Guo AK,Vedula SR,Harada I,Chiam KH,Ishihama Y,Lim CT,Sawada Y,Sokabe M

T-Cell-Specific Deletion of Map3k1 Reveals the Critical Role for Mekk1 and Jnks in Cdkn1b-Dependent Proliferative Expansion.
Cell reports14(3)449-457(2016 Jan)
Suddason T,Anwar S,Charlaftis N,Gallagher E

MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells.
Disease models & mechanisms8(12)1543-53(2015 Dec)
Yousaf R,Meng Q,Hufnagel RB,Xia Y,Puligilla C,Ahmed ZM,Riazuddin S

The goya mouse mutant reveals distinct newly identified roles for MAP3K1 in the development and survival of cochlear sensory hair cells.
Disease models & mechanisms8(12)1555-68(2015 Dec)
Parker A,Cross SH,Jackson IJ,Hardisty-Hughes R,Morse S,Nicholson G,Coghill E,Bowl MR,Brown SD

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Map3k1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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