MAP3K1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	4214
Full Name	mitogen-activated protein kinase kinase kinase 1
Synonyms	MAPKKK1,MEKK,MEKK 1,MEKK1,SRXY6
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 1 Names mitogen-activated protein kinase kinase kinase 1 MAP/ERK kinase kinase 1 MAPK/ERK kinase kinase 1 MEK kinase 1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012].
Disorder MIM:	600982
Disorder Html:	46XY sex reversal 6, 613762 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005921.1	NP_005912.1	mitogen-activated protein kinase kinase kinase 1
XM_017009484.1	XP_016864973.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
NM_005921.2	NP_005912.1	mitogen-activated protein kinase kinase kinase 1
XM_017009485.1	XP_016864974.1	mitogen-activated protein kinase kinase kinase 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K1	NP_005912.1
Rattus norvegicus (Norway rat)	LOC100912399	XP_006231988.1
	MAP3K1	XP_002804414.1
Canis lupus familiaris (dog)	MAP3K1	XP_535240.4
Xenopus tropicalis (tropical clawed frog)	map3k1	XP_002940279.2
Rattus norvegicus (Norway rat)	Map3k1	NP_446339.1
Pan troglodytes (chimpanzee)	MAP3K1	XP_003310777.1
Bos taurus (cattle)	MAP3K1	NP_001192835.1
Mus musculus (house mouse)	Map3k1	NP_036075.2
Gallus gallus (chicken)	MAP3K1	XP_424734.3
Danio rerio (zebrafish)	map3k1	XP_002663531.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Variation in MAP3K1 Associates with Ventilator-Free Days in Acute Respiratory Distress Syndrome.
Morrell ED, O'Mahony DS, Glavan BJ, Harju-Baker S, Nguyen C, Gunderson S, Abrahamson A, Radella F 2nd, Rona G, Black RA, Wurfel MM
American journal of respiratory cell and molecular biology58(1)117-125(2018 Jan)

MAP3K1-related gonadal dysgenesis: Six new cases and review of the literature.
Granados A, Alaniz VI, Mohnach L, Barseghyan H, Vilain E, Ostrer H, Quint EH, Chen M, Keegan CE
American journal of medical genetics. Part C, Seminars in medical genetics175(2)253-259(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification of a MAP3K1 variant should prompt an evaluation for disorders of sex development in female siblings of the proband
Title: MAP3K1-related gonadal dysgenesis: Six new cases and review of the literature.

Polymorphism of MAP3K1 is associated with breast cancer.
Title: Polymorphisms of ESR1, UGT1A1, HCN1, MAP3K1 and CYP2B6 are associated with the prognosis of hormone receptor-positive early breast cancer.

SNP variants at the MAP3K1/SETD9 gene boundary associate with somatic PIK3CA variants in breast cancers.
Title: SNP variants at the MAP3K1/SETD9 locus 5q11.2 associate with somatic PIK3CA variants in breast cancers.

BAALC conferred chemoresistance in acute myeloid leukemia cells by upregulating ATP-binding cassette proteins in an ERK-dependent manner, which can be therapeutically targeted by MEK inhibitor
Title: BAALC potentiates oncogenic ERK pathway through interactions with MEKK1 and KLF4.

CSN6 positively regulates c-Jun in a MEKK1-dependent manner
Title: CSN6 positively regulates c-Jun in a MEKK1-dependent manner.

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu95890	XM_017009485.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu95889	XM_017009484.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu24943	NM_005921.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
View Old Accession Versions >>
OHu24943	NM_005921.1	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_017009485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864974.1 Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu95890
Clone ID Related Accession (Same CDS sequence)	XM_017009485.1
Accession Version	XM_017009485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACTCT CAAAGGGTTG CACAAGATGG ATGATCGTCC AGAGGAACGA 
ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGTTGGAA 
AGGAGAAATA GGCGAGGGCC TGTGGTGGTA AAACCAATCC CAGTTAAAGG AGATGGATCT 
GAAATGAATC ACTTAGCAGC TGAGTCTCCA GGAGAGGTCC AGGCAAGTGC GGCTTCACCA 
GCTTCCAAAG GCCGACGCAG TCCTTCTCCT GGCAACTCCC CATCAGGTCG CACAGTGAAA 
TCAGAATCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCAG TGCCTTTTCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCTGAGGAA 
ACAAACCGCC GTGTTAACAA AGTGATGCGG GCCAGACTGT ACTTACTGCA GCAGATAGGG 
CCTAACTCTT TCCTGATTGG AGGAGACAGC CCAGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGC ACGTGGAACA TTCTGTATTC ATCTGCTATT TGTGATGCTC 
CGGGTGTTTC AACTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACGTCTAG TTCAGAAAAC AGCATAAAGG ATGAAGAGGA ACAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC 
AGGAACAAGC TGCACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGTAG AAGAAATAGA 
GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CTCATGATTT CTACAGCCAC 
GAGTTGTCAA GTCCTGTGGA TTCCCCTTCT TCCCTCAGAG CTGCACAGCA GCAAACCGTA 
CAGCAGCAGC CTTTGGCTGG ATCACGAAGG AATCAAGAGA GCAATTTTAA CCTTACTCAT 
TATGGAACTC AGCAAATCCC TCCTGCTTAC AAAGATTTAG CTGAGCCATG GATTCAGGTG 
TTTGGAATGG AACTCGTTGG CTGCTTATTT TCTAGAAACT GGAATGTGAG AGAGATGGCC 
CTCAGGCGTC TTTCCCATGA TGTCAGTGGG GCCCTGCTGT TGGCAAATGG GGAGAGCACT 
GGAAATTCTG GGGGCAGCAG TGGAAGCAGC CCGAGTGGGG GAGCCACCAG TGGGTCTTCC 
CAGACCAGTA TCTCAGGAGA TGTGGTGGAG GCATGCTGCA GCGTTCTGTC AATGGTCTGT 
GCTGACCCTG TCTACAAAGT GTACGTTGCT GCTTTAAAAA CATTGAGAGC CATGCTGGTA 
TATACTCCTT GCCACAGTTT AGCGGAAAGA ATCAAACTTC AGAGACTTCT CCAGCCAGTT 
GTAGACACCA TCCTAGTCAA ATGTGCAGAT GCCAATAGCC GCACAAGTCA GCTGTCCATA 
TCAACACTGT TGGAACTGTG CAAAGGCCAA GCAGGAGAGT TGGCAGTTGG CAGAGAAATA 
CTAAAAGCTG GATCCATTGG TATTGGTGGT GTTGATTATG TCTTAAATTG TATTCTTGGA 
AACCAAACTG AATCAAACAA TTGGCAAGAA CTTCTTGGCC GCCTTTGTCT TATAGATAGA 
CTGTTGTTGG AATTTCCTGC TGAATTTTAT CCTCATATTG TCAGTACTGA TGTTTCACAA 
GCTGAGCCTG TTGAAATCAG GTATAAGAAG CTGCTGTCCC TCTTAACCTT TGCTTTGCAG 
TCCATTGATA ATTCCCACTC AATGGTTGGC AAACTTTCCA GAAGGATCTA CTTGAGTTCT 
GCAAGAATGG TTACTACAGT ACCCCATGTG TTTTCAAAAC TGTTAGAAAT GCTGAGTGTT 
TCCAGTTCCA CTCACTTCAC CAGGATGCGT CGCCGTTTGA TGGCTATTGC AGATGAGGTG 
GAAATTGCCG AAGCCATCCA GTTGGGCGTA GAAGACACTT TGGATGGTCA ACAGGACAGC 
TTCTTGCAGG CATCTGTTCC CAACAACTAT CTGGAAACCA CAGAGAACAG TTCCCCTGAG 
TGCACAGTCC ATTTAGAGAA AACTGGAAAA GGATTATGTG CTACAAAATT GAGTGCCAGT 
TCAGAGGACA TTTCTGAGAG ACTGGCCAGC ATTTCAGTAG GACCTTCTAG TTCAACAACA 
ACAACAACAA CAACAACAGA GCAACCAAAG CCAATGGTTC AAACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCCTC TCCTTTATCT CATCATTCCC AATTAATGTT TCCAGCCTTG 
TCAACCCCTT CTTCTTCTAC CCCATCTGTA CCAGCTGGCA CTGCAACAGA TGTCTCTAAG 
CATAGACTTC AGGGATTCAT TCCCTGCAGA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAATTCCA CAGAAACTGT CCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCTTGCCC TCCAGTAACA TACACAGGCC AAAGCCATCT 
AGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATCCCT CAAAAAATAG CATGACACTT 
GATCTGAACA GTAGTTCCAA ATGTGATGAC AGCTTTGGCT GTAGCAGCAA TAGTAGTAAT 
GCTGTTATAC CCAGTGACGA GACAGTGTTC ACCCCAGTAG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AGCTCAACTC CAGTATTGAG GACCTTCTTG AAGCATCTAT GCCTTCAAGT 
GATACAACAG TAACTTTTAA GTCAGAAGTT GCTGTCCTGT CTCCTGAAAA GGCTGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAGAA GATGGAAGCT 
GAAGAAGAAG AAGCTTTAGC AATTGCCATG GCAATGTCAG CGTCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AGCTGCAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TACCAGGACA TACCAAAGCA AAACAACCGT ATAGAGAAGA CACTGAATGG 
CTGAAAGGTC AACAGATAGG CCTTGGAGCA TTTTCTTCTT GTTATCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACTTATG TCAGAAACAC ATCTTCTGAG 
CAAGAAGAAG TAGTAGAAGC ACTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAACATCA TTAGGATGTT GGGAGCCACG TGTGAGAAGA GCAATTACAA TCTCTTCATT 
GAATGGATGG CAGGGGGATC GGTGGCTCAT TTGCTGAGTA AATATGGAGC CTTCAAAGAA 
TCAGTAGTTA TTAACTACAC TGAACAGTTA CTCCGTGGCC TTTCGTATCT CCATGAAAAC 
CAAATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC TGGTCAGAGA 
CTAAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAATTACT GGGGACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT 
CAACAGTATG GAAGGAGCTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCAAAAC CACCATGGAA TGCAGAAAAA CACTCCAATC ATCTTGCTTT GATATTTAAG 
ATTGCTAGTG CAACTACTGC TCCATCGATC CCTTCACATT TGTCTCCTGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCATCAAG AGAGCTACTG 
AAGCATCCAG TCTTTCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864974.1
CDS	430..4479
Translation	MENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPVKGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQTSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017009485.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009485.1

ORF Insert Sequence:

ATGGAGAATA AAGAAACTCT CAAAGGGTTG CACAAGATGG ATGATCGTCC AGAGGAACGA 
ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGTTGGAA 
AGGAGAAATA GGCGAGGGCC TGTGGTGGTA AAACCAATCC CAGTTAAAGG AGATGGATCT 
GAAATGAATC ACTTAGCAGC TGAGTCTCCA GGAGAGGTCC AGGCAAGTGC GGCTTCACCA 
GCTTCCAAAG GCCGACGCAG TCCTTCTCCT GGCAACTCCC CATCAGGTCG CACAGTGAAA 
TCAGAATCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCAG TGCCTTTTCA GAGTGGCAGA 
ATCACACCAC CCCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCTGAGGAA 
ACAAACCGCC GTGTTAACAA AGTGATGCGG GCCAGACTGT ACTTACTGCA GCAGATAGGG 
CCTAACTCTT TCCTGATTGG AGGAGACAGC CCAGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGC ACGTGGAACA TTCTGTATTC ATCTGCTATT TGTGATGCTC 
CGGGTGTTTC AACTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACGTCTAG TTCAGAAAAC AGCATAAAGG ATGAAGAGGA ACAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC 
AGGAACAAGC TGCACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGTAG AAGAAATAGA 
GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CTCATGATTT CTACAGCCAC 
GAGTTGTCAA GTCCTGTGGA TTCCCCTTCT TCCCTCAGAG CTGCACAGCA GCAAACCGTA 
CAGCAGCAGC CTTTGGCTGG ATCACGAAGG AATCAAGAGA GCAATTTTAA CCTTACTCAT 
TATGGAACTC AGCAAATCCC TCCTGCTTAC AAAGATTTAG CTGAGCCATG GATTCAGGTG 
TTTGGAATGG AACTCGTTGG CTGCTTATTT TCTAGAAACT GGAATGTGAG AGAGATGGCC 
CTCAGGCGTC TTTCCCATGA TGTCAGTGGG GCCCTGCTGT TGGCAAATGG GGAGAGCACT 
GGAAATTCTG GGGGCAGCAG TGGAAGCAGC CCGAGTGGGG GAGCCACCAG TGGGTCTTCC 
CAGACCAGTA TCTCAGGAGA TGTGGTGGAG GCATGCTGCA GCGTTCTGTC AATGGTCTGT 
GCTGACCCTG TCTACAAAGT GTACGTTGCT GCTTTAAAAA CATTGAGAGC CATGCTGGTA 
TATACTCCTT GCCACAGTTT AGCGGAAAGA ATCAAACTTC AGAGACTTCT CCAGCCAGTT 
GTAGACACCA TCCTAGTCAA ATGTGCAGAT GCCAATAGCC GCACAAGTCA GCTGTCCATA 
TCAACACTGT TGGAACTGTG CAAAGGCCAA GCAGGAGAGT TGGCAGTTGG CAGAGAAATA 
CTAAAAGCTG GATCCATTGG TATTGGTGGT GTTGATTATG TCTTAAATTG TATTCTTGGA 
AACCAAACTG AATCAAACAA TTGGCAAGAA CTTCTTGGCC GCCTTTGTCT TATAGATAGA 
CTGTTGTTGG AATTTCCTGC TGAATTTTAT CCTCATATTG TCAGTACTGA TGTTTCACAA 
GCTGAGCCTG TTGAAATCAG GTATAAGAAG CTGCTGTCCC TCTTAACCTT TGCTTTGCAG 
TCCATTGATA ATTCCCACTC AATGGTTGGC AAACTTTCCA GAAGGATCTA CTTGAGTTCT 
GCAAGAATGG TTACTACAGT ACCCCATGTG TTTTCAAAAC TGTTAGAAAT GCTGAGTGTT 
TCCAGTTCCA CTCACTTCAC CAGGATGCGT CGCCGTTTGA TGGCTATTGC AGATGAGGTG 
GAAATTGCCG AAGCCATCCA GTTGGGCGTA GAAGACACTT TGGATGGTCA ACAGGACAGC 
TTCTTGCAGG CATCTGTTCC CAACAACTAT CTGGAAACCA CAGAGAACAG TTCCCCTGAG 
TGCACAGTCC ATTTAGAGAA AACTGGAAAA GGATTATGTG CTACAAAATT GAGTGCCAGT 
TCAGAGGACA TTTCTGAGAG ACTGGCCAGC ATTTCAGTAG GACCTTCTAG TTCAACAACA 
ACAACAACAA CAACAACAGA GCAACCAAAG CCAATGGTTC AAACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCCTC TCCTTTATCT CATCATTCCC AATTAATGTT TCCAGCCTTG 
TCAACCCCTT CTTCTTCTAC CCCATCTGTA CCAGCTGGCA CTGCAACAGA TGTCTCTAAG 
CATAGACTTC AGGGATTCAT TCCCTGCAGA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAATTCCA CAGAAACTGT CCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCTTGCCC TCCAGTAACA TACACAGGCC AAAGCCATCT 
AGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATCCCT CAAAAAATAG CATGACACTT 
GATCTGAACA GTAGTTCCAA ATGTGATGAC AGCTTTGGCT GTAGCAGCAA TAGTAGTAAT 
GCTGTTATAC CCAGTGACGA GACAGTGTTC ACCCCAGTAG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AGCTCAACTC CAGTATTGAG GACCTTCTTG AAGCATCTAT GCCTTCAAGT 
GATACAACAG TAACTTTTAA GTCAGAAGTT GCTGTCCTGT CTCCTGAAAA GGCTGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAGAA GATGGAAGCT 
GAAGAAGAAG AAGCTTTAGC AATTGCCATG GCAATGTCAG CGTCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AGCTGCAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TACCAGGACA TACCAAAGCA AAACAACCGT ATAGAGAAGA CACTGAATGG 
CTGAAAGGTC AACAGATAGG CCTTGGAGCA TTTTCTTCTT GTTATCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACTTATG TCAGAAACAC ATCTTCTGAG 
CAAGAAGAAG TAGTAGAAGC ACTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAACATCA TTAGGATGTT GGGAGCCACG TGTGAGAAGA GCAATTACAA TCTCTTCATT 
GAATGGATGG CAGGGGGATC GGTGGCTCAT TTGCTGAGTA AATATGGAGC CTTCAAAGAA 
TCAGTAGTTA TTAACTACAC TGAACAGTTA CTCCGTGGCC TTTCGTATCT CCATGAAAAC 
CAAATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC TGGTCAGAGA 
CTAAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAATTACT GGGGACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT 
CAACAGTATG GAAGGAGCTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCAAAAC CACCATGGAA TGCAGAAAAA CACTCCAATC ATCTTGCTTT GATATTTAAG 
ATTGCTAGTG CAACTACTGC TCCATCGATC CCTTCACATT TGTCTCCTGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCATCAAG AGAGCTACTG 
AAGCATCCAG TCTTTCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_017009484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864973.1 Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu95889
Clone ID Related Accession (Same CDS sequence)	XM_017009484.1
Accession Version	XM_017009484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTCT GCAAGGTGGT GGTCTTGAGT GGTGGAGAAA AAGATCATGA GTGCACAAGC 
TGTTTCTGTC GTCGTGAGAT GGAGAATAAA GAAACTCTCA AAGGGTTGCA CAAGATGGAT 
GATCGTCCAG AGGAACGAAT GATCAGGGAG AAACTGAAGG CAACCTGTAT GCCAGCCTGG 
AAGCACGAAT GGTTGGAAAG GAGAAATAGG CGAGGGCCTG TGGTGGTAAA ACCAATCCCA 
GTTAAAGGAG ATGGATCTGA AATGAATCAC TTAGCAGCTG AGTCTCCAGG AGAGGTCCAG 
GCAAGTGCGG CTTCACCAGC TTCCAAAGGC CGACGCAGTC CTTCTCCTGG CAACTCCCCA 
TCAGGTCGCA CAGTGAAATC AGAATCTCCA GGAGTAAGGA GAAAAAGAGT TTCCCCAGTG 
CCTTTTCAGA GTGGCAGAAT CACACCACCC CGAAGAGCCC CTTCACCAGA TGGCTTCTCA 
CCATATAGCC CTGAGGAAAC AAACCGCCGT GTTAACAAAG TGATGCGGGC CAGACTGTAC 
TTACTGCAGC AGATAGGGCC TAACTCTTTC CTGATTGGAG GAGACAGCCC AGACAATAAA 
TACCGGGTGT TTATTGGGCC TCAGAACTGC AGCTGTGCAC GTGGAACATT CTGTATTCAT 
CTGCTATTTG TGATGCTCCG GGTGTTTCAA CTAGAACCTT CAGACCCAAT GTTATGGAGA 
AAAACTTTAA AGAATTTTGA GGTTGAGAGT TTGTTCCAGA AATATCACAG TAGGCGTAGC 
TCAAGGATCA AAGCTCCATC TCGTAACACC ATCCAGAAGT TTGTTTCACG CATGTCAAAT 
TCTCATACAT TGTCATCATC TAGTACTTCT ACGTCTAGTT CAGAAAACAG CATAAAGGAT 
GAAGAGGAAC AGATGTGTCC TATTTGCTTG TTGGGCATGC TTGATGAAGA AAGTCTTACA 
GTGTGTGAAG ACGGCTGCAG GAACAAGCTG CACCACCACT GCATGTCAAT TTGGGCAGAA 
GAGTGTAGAA GAAATAGAGA ACCTTTAATA TGTCCCCTTT GTAGATCTAA GTGGAGATCT 
CATGATTTCT ACAGCCACGA GTTGTCAAGT CCTGTGGATT CCCCTTCTTC CCTCAGAGCT 
GCACAGCAGC AAACCGTACA GCAGCAGCCT TTGGCTGGAT CACGAAGGAA TCAAGAGAGC 
AATTTTAACC TTACTCATTA TGGAACTCAG CAAATCCCTC CTGCTTACAA AGATTTAGCT 
GAGCCATGGA TTCAGGTGTT TGGAATGGAA CTCGTTGGCT GCTTATTTTC TAGAAACTGG 
AATGTGAGAG AGATGGCCCT CAGGCGTCTT TCCCATGATG TCAGTGGGGC CCTGCTGTTG 
GCAAATGGGG AGAGCACTGG AAATTCTGGG GGCAGCAGTG GAAGCAGCCC GAGTGGGGGA 
GCCACCAGTG GGTCTTCCCA GACCAGTATC TCAGGAGATG TGGTGGAGGC ATGCTGCAGC 
GTTCTGTCAA TGGTCTGTGC TGACCCTGTC TACAAAGTGT ACGTTGCTGC TTTAAAAACA 
TTGAGAGCCA TGCTGGTATA TACTCCTTGC CACAGTTTAG CGGAAAGAAT CAAACTTCAG 
AGACTTCTCC AGCCAGTTGT AGACACCATC CTAGTCAAAT GTGCAGATGC CAATAGCCGC 
ACAAGTCAGC TGTCCATATC AACACTGTTG GAACTGTGCA AAGGCCAAGC AGGAGAGTTG 
GCAGTTGGCA GAGAAATACT AAAAGCTGGA TCCATTGGTA TTGGTGGTGT TGATTATGTC 
TTAAATTGTA TTCTTGGAAA CCAAACTGAA TCAAACAATT GGCAAGAACT TCTTGGCCGC 
CTTTGTCTTA TAGATAGACT GTTGTTGGAA TTTCCTGCTG AATTTTATCC TCATATTGTC 
AGTACTGATG TTTCACAAGC TGAGCCTGTT GAAATCAGGT ATAAGAAGCT GCTGTCCCTC 
TTAACCTTTG CTTTGCAGTC CATTGATAAT TCCCACTCAA TGGTTGGCAA ACTTTCCAGA 
AGGATCTACT TGAGTTCTGC AAGAATGGTT ACTACAGTAC CCCATGTGTT TTCAAAACTG 
TTAGAAATGC TGAGTGTTTC CAGTTCCACT CACTTCACCA GGATGCGTCG CCGTTTGATG 
GCTATTGCAG ATGAGGTGGA AATTGCCGAA GCCATCCAGT TGGGCGTAGA AGACACTTTG 
GATGGTCAAC AGGACAGCTT CTTGCAGGCA TCTGTTCCCA ACAACTATCT GGAAACCACA 
GAGAACAGTT CCCCTGAGTG CACAGTCCAT TTAGAGAAAA CTGGAAAAGG ATTATGTGCT 
ACAAAATTGA GTGCCAGTTC AGAGGACATT TCTGAGAGAC TGGCCAGCAT TTCAGTAGGA 
CCTTCTAGTT CAACAACAAC AACAACAACA ACAACAGAGC AACCAAAGCC AATGGTTCAA 
ACAAAAGGCA GACCCCACAG TCAGTGTTTG AACTCCTCTC CTTTATCTCA TCATTCCCAA 
TTAATGTTTC CAGCCTTGTC AACCCCTTCT TCTTCTACCC CATCTGTACC AGCTGGCACT 
GCAACAGATG TCTCTAAGCA TAGACTTCAG GGATTCATTC CCTGCAGAAT ACCTTCTGCA 
TCTCCTCAAA CACAGCGCAA GTTTTCTCTA CAATTCCACA GAAACTGTCC TGAAAACAAA 
GACTCAGATA AACTTTCCCC AGTCTTTACT CAGTCAAGAC CCTTGCCCTC CAGTAACATA 
CACAGGCCAA AGCCATCTAG ACCTACCCCA GGTAATACAA GTAAACAGGG AGATCCCTCA 
AAAAATAGCA TGACACTTGA TCTGAACAGT AGTTCCAAAT GTGATGACAG CTTTGGCTGT 
AGCAGCAATA GTAGTAATGC TGTTATACCC AGTGACGAGA CAGTGTTCAC CCCAGTAGAG 
GAGAAATGCA GATTAGATGT CAATACAGAG CTCAACTCCA GTATTGAGGA CCTTCTTGAA 
GCATCTATGC CTTCAAGTGA TACAACAGTA ACTTTTAAGT CAGAAGTTGC TGTCCTGTCT 
CCTGAAAAGG CTGAAAATGA TGATACCTAC AAAGATGATG TGAATCATAA TCAAAAGTGC 
AAAGAGAAGA TGGAAGCTGA AGAAGAAGAA GCTTTAGCAA TTGCCATGGC AATGTCAGCG 
TCTCAGGATG CCCTCCCCAT AGTTCCTCAG CTGCAGGTTG AAAATGGAGA AGATATCATC 
ATTATTCAAC AGGATACACC AGAGACTCTA CCAGGACATA CCAAAGCAAA ACAACCGTAT 
AGAGAAGACA CTGAATGGCT GAAAGGTCAA CAGATAGGCC TTGGAGCATT TTCTTCTTGT 
TATCAGGCTC AAGATGTGGG AACTGGAACT TTAATGGCTG TTAAACAGGT GACTTATGTC 
AGAAACACAT CTTCTGAGCA AGAAGAAGTA GTAGAAGCAC TAAGAGAAGA GATAAGAATG 
ATGAGCCATC TGAATCATCC AAACATCATT AGGATGTTGG GAGCCACGTG TGAGAAGAGC 
AATTACAATC TCTTCATTGA ATGGATGGCA GGGGGATCGG TGGCTCATTT GCTGAGTAAA 
TATGGAGCCT TCAAAGAATC AGTAGTTATT AACTACACTG AACAGTTACT CCGTGGCCTT 
TCGTATCTCC ATGAAAACCA AATCATTCAC AGAGATGTCA AAGGTGCCAA TTTGCTAATT 
GACAGCACTG GTCAGAGACT AAGAATTGCA GATTTTGGAG CTGCAGCCAG GTTGGCATCA 
AAAGGAACTG GTGCAGGAGA GTTTCAGGGA CAATTACTGG GGACAATTGC ATTTATGGCA 
CCTGAGGTAC TAAGAGGTCA ACAGTATGGA AGGAGCTGTG ATGTATGGAG TGTTGGCTGT 
GCTATTATAG AAATGGCTTG TGCAAAACCA CCATGGAATG CAGAAAAACA CTCCAATCAT 
CTTGCTTTGA TATTTAAGAT TGCTAGTGCA ACTACTGCTC CATCGATCCC TTCACATTTG 
TCTCCTGGTT TACGAGATGT GGCTCTTCGT TGTTTAGAAC TTCAACCTCA GGACAGACCT 
CCATCAAGAG AGCTACTGAA GCATCCAGTC TTTCGTACTA CATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864973.1
CDS	3624..7751
Translation	MNFCKVVVLSGGEKDHECTSCFCRREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPVKGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQTSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_017009484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009484.1

ORF Insert Sequence:

ATGAACTTCT GCAAGGTGGT GGTCTTGAGT GGTGGAGAAA AAGATCATGA GTGCACAAGC 
TGTTTCTGTC GTCGTGAGAT GGAGAATAAA GAAACTCTCA AAGGGTTGCA CAAGATGGAT 
GATCGTCCAG AGGAACGAAT GATCAGGGAG AAACTGAAGG CAACCTGTAT GCCAGCCTGG 
AAGCACGAAT GGTTGGAAAG GAGAAATAGG CGAGGGCCTG TGGTGGTAAA ACCAATCCCA 
GTTAAAGGAG ATGGATCTGA AATGAATCAC TTAGCAGCTG AGTCTCCAGG AGAGGTCCAG 
GCAAGTGCGG CTTCACCAGC TTCCAAAGGC CGACGCAGTC CTTCTCCTGG CAACTCCCCA 
TCAGGTCGCA CAGTGAAATC AGAATCTCCA GGAGTAAGGA GAAAAAGAGT TTCCCCAGTG 
CCTTTTCAGA GTGGCAGAAT CACACCACCC CGAAGAGCCC CTTCACCAGA TGGCTTCTCA 
CCATATAGCC CTGAGGAAAC AAACCGCCGT GTTAACAAAG TGATGCGGGC CAGACTGTAC 
TTACTGCAGC AGATAGGGCC TAACTCTTTC CTGATTGGAG GAGACAGCCC AGACAATAAA 
TACCGGGTGT TTATTGGGCC TCAGAACTGC AGCTGTGCAC GTGGAACATT CTGTATTCAT 
CTGCTATTTG TGATGCTCCG GGTGTTTCAA CTAGAACCTT CAGACCCAAT GTTATGGAGA 
AAAACTTTAA AGAATTTTGA GGTTGAGAGT TTGTTCCAGA AATATCACAG TAGGCGTAGC 
TCAAGGATCA AAGCTCCATC TCGTAACACC ATCCAGAAGT TTGTTTCACG CATGTCAAAT 
TCTCATACAT TGTCATCATC TAGTACTTCT ACGTCTAGTT CAGAAAACAG CATAAAGGAT 
GAAGAGGAAC AGATGTGTCC TATTTGCTTG TTGGGCATGC TTGATGAAGA AAGTCTTACA 
GTGTGTGAAG ACGGCTGCAG GAACAAGCTG CACCACCACT GCATGTCAAT TTGGGCAGAA 
GAGTGTAGAA GAAATAGAGA ACCTTTAATA TGTCCCCTTT GTAGATCTAA GTGGAGATCT 
CATGATTTCT ACAGCCACGA GTTGTCAAGT CCTGTGGATT CCCCTTCTTC CCTCAGAGCT 
GCACAGCAGC AAACCGTACA GCAGCAGCCT TTGGCTGGAT CACGAAGGAA TCAAGAGAGC 
AATTTTAACC TTACTCATTA TGGAACTCAG CAAATCCCTC CTGCTTACAA AGATTTAGCT 
GAGCCATGGA TTCAGGTGTT TGGAATGGAA CTCGTTGGCT GCTTATTTTC TAGAAACTGG 
AATGTGAGAG AGATGGCCCT CAGGCGTCTT TCCCATGATG TCAGTGGGGC CCTGCTGTTG 
GCAAATGGGG AGAGCACTGG AAATTCTGGG GGCAGCAGTG GAAGCAGCCC GAGTGGGGGA 
GCCACCAGTG GGTCTTCCCA GACCAGTATC TCAGGAGATG TGGTGGAGGC ATGCTGCAGC 
GTTCTGTCAA TGGTCTGTGC TGACCCTGTC TACAAAGTGT ACGTTGCTGC TTTAAAAACA 
TTGAGAGCCA TGCTGGTATA TACTCCTTGC CACAGTTTAG CGGAAAGAAT CAAACTTCAG 
AGACTTCTCC AGCCAGTTGT AGACACCATC CTAGTCAAAT GTGCAGATGC CAATAGCCGC 
ACAAGTCAGC TGTCCATATC AACACTGTTG GAACTGTGCA AAGGCCAAGC AGGAGAGTTG 
GCAGTTGGCA GAGAAATACT AAAAGCTGGA TCCATTGGTA TTGGTGGTGT TGATTATGTC 
TTAAATTGTA TTCTTGGAAA CCAAACTGAA TCAAACAATT GGCAAGAACT TCTTGGCCGC 
CTTTGTCTTA TAGATAGACT GTTGTTGGAA TTTCCTGCTG AATTTTATCC TCATATTGTC 
AGTACTGATG TTTCACAAGC TGAGCCTGTT GAAATCAGGT ATAAGAAGCT GCTGTCCCTC 
TTAACCTTTG CTTTGCAGTC CATTGATAAT TCCCACTCAA TGGTTGGCAA ACTTTCCAGA 
AGGATCTACT TGAGTTCTGC AAGAATGGTT ACTACAGTAC CCCATGTGTT TTCAAAACTG 
TTAGAAATGC TGAGTGTTTC CAGTTCCACT CACTTCACCA GGATGCGTCG CCGTTTGATG 
GCTATTGCAG ATGAGGTGGA AATTGCCGAA GCCATCCAGT TGGGCGTAGA AGACACTTTG 
GATGGTCAAC AGGACAGCTT CTTGCAGGCA TCTGTTCCCA ACAACTATCT GGAAACCACA 
GAGAACAGTT CCCCTGAGTG CACAGTCCAT TTAGAGAAAA CTGGAAAAGG ATTATGTGCT 
ACAAAATTGA GTGCCAGTTC AGAGGACATT TCTGAGAGAC TGGCCAGCAT TTCAGTAGGA 
CCTTCTAGTT CAACAACAAC AACAACAACA ACAACAGAGC AACCAAAGCC AATGGTTCAA 
ACAAAAGGCA GACCCCACAG TCAGTGTTTG AACTCCTCTC CTTTATCTCA TCATTCCCAA 
TTAATGTTTC CAGCCTTGTC AACCCCTTCT TCTTCTACCC CATCTGTACC AGCTGGCACT 
GCAACAGATG TCTCTAAGCA TAGACTTCAG GGATTCATTC CCTGCAGAAT ACCTTCTGCA 
TCTCCTCAAA CACAGCGCAA GTTTTCTCTA CAATTCCACA GAAACTGTCC TGAAAACAAA 
GACTCAGATA AACTTTCCCC AGTCTTTACT CAGTCAAGAC CCTTGCCCTC CAGTAACATA 
CACAGGCCAA AGCCATCTAG ACCTACCCCA GGTAATACAA GTAAACAGGG AGATCCCTCA 
AAAAATAGCA TGACACTTGA TCTGAACAGT AGTTCCAAAT GTGATGACAG CTTTGGCTGT 
AGCAGCAATA GTAGTAATGC TGTTATACCC AGTGACGAGA CAGTGTTCAC CCCAGTAGAG 
GAGAAATGCA GATTAGATGT CAATACAGAG CTCAACTCCA GTATTGAGGA CCTTCTTGAA 
GCATCTATGC CTTCAAGTGA TACAACAGTA ACTTTTAAGT CAGAAGTTGC TGTCCTGTCT 
CCTGAAAAGG CTGAAAATGA TGATACCTAC AAAGATGATG TGAATCATAA TCAAAAGTGC 
AAAGAGAAGA TGGAAGCTGA AGAAGAAGAA GCTTTAGCAA TTGCCATGGC AATGTCAGCG 
TCTCAGGATG CCCTCCCCAT AGTTCCTCAG CTGCAGGTTG AAAATGGAGA AGATATCATC 
ATTATTCAAC AGGATACACC AGAGACTCTA CCAGGACATA CCAAAGCAAA ACAACCGTAT 
AGAGAAGACA CTGAATGGCT GAAAGGTCAA CAGATAGGCC TTGGAGCATT TTCTTCTTGT 
TATCAGGCTC AAGATGTGGG AACTGGAACT TTAATGGCTG TTAAACAGGT GACTTATGTC 
AGAAACACAT CTTCTGAGCA AGAAGAAGTA GTAGAAGCAC TAAGAGAAGA GATAAGAATG 
ATGAGCCATC TGAATCATCC AAACATCATT AGGATGTTGG GAGCCACGTG TGAGAAGAGC 
AATTACAATC TCTTCATTGA ATGGATGGCA GGGGGATCGG TGGCTCATTT GCTGAGTAAA 
TATGGAGCCT TCAAAGAATC AGTAGTTATT AACTACACTG AACAGTTACT CCGTGGCCTT 
TCGTATCTCC ATGAAAACCA AATCATTCAC AGAGATGTCA AAGGTGCCAA TTTGCTAATT 
GACAGCACTG GTCAGAGACT AAGAATTGCA GATTTTGGAG CTGCAGCCAG GTTGGCATCA 
AAAGGAACTG GTGCAGGAGA GTTTCAGGGA CAATTACTGG GGACAATTGC ATTTATGGCA 
CCTGAGGTAC TAAGAGGTCA ACAGTATGGA AGGAGCTGTG ATGTATGGAG TGTTGGCTGT 
GCTATTATAG AAATGGCTTG TGCAAAACCA CCATGGAATG CAGAAAAACA CTCCAATCAT 
CTTGCTTTGA TATTTAAGAT TGCTAGTGCA ACTACTGCTC CATCGATCCC TTCACATTTG 
TCTCCTGGTT TACGAGATGT GGCTCTTCGT TGTTTAGAAC TTCAACCTCA GGACAGACCT 
CCATCAAGAG AGCTACTGAA GCATCCAGTC TTTCGTACTA CATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( NM_005921.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_005912.1 Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

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CloneID	OHu24943
Clone ID Related Accession (Same CDS sequence)	NM_005921.1 , NM_005921.2
Accession Version	NM_005921.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008937.7, AF042838.1, BU194120.1, DA889202.1 and AA602425.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 27, 2007 this sequence version replaced XM_042066.11, XM_001128827.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC 
GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT 
GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG 
AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC 
GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG 
GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA 
GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA 
GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG 
GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC 
ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG 
AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG 
CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT 
GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA 
AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC 
TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG 
CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC 
CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG 
ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA 
GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG 
GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT 
GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA 
ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC 
ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC 
CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC 
AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT 
ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT 
ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT 
GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT 
GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC 
TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG 
CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA 
GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA 
CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT 
TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG 
AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT 
TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC 
AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT 
CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT 
GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA 
ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT 
AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA 
AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC 
ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT 
AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC 
AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG 
CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG 
GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG 
ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT 
GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA 
CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC 
ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT 
CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA 
TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT 
CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT 
CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC 
TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC 
CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT 
GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT 
GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA 
CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA 
GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG 
ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT 
TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA 
GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005912.1
CDS	502..5040
Translation	MAAAAGNRASSSGFPGARATSPEAGGGGGALKASSAPAAAAGLLREAGSGGRERADWRRRQLRKVRSVELDQLPEQPLFLAASPPASSTSPSPEPADAAGSGTGFQPVAVPPPHGAASRGGAHLTESVAAPDSGASSPAAAEPGEKRAPAAEPSPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPVKGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQTSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	NM_005921.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005921.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC 
GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT 
GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG 
AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC 
GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG 
GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA 
GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA 
GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG 
GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC 
ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG 
AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG 
CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT 
GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA 
AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC 
TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG 
CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC 
CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG 
ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA 
GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG 
GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT 
GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA 
ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC 
ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC 
CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC 
AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT 
ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT 
ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT 
GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT 
GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC 
TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG 
CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA 
GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA 
CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT 
TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG 
AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT 
TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC 
AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT 
CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT 
GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA 
ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT 
AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA 
AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC 
ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT 
AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC 
AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG 
CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG 
GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG 
ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT 
GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA 
CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC 
ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT 
CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA 
TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT 
CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT 
CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC 
TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC 
CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT 
GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT 
GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA 
CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA 
GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG 
ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT 
TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA 
GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( NM_005921.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005912.1 Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

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CloneID	OHu24943
Clone ID Related Accession (Same CDS sequence)	NM_005921.1 , NM_005921.2
Accession Version	NM_005921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008937.7, AF042838.1, BU194120.1, DA889202.1 and AA602425.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005921.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399503.4/ ENSP00000382423.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC 
GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT 
GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG 
AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC 
GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG 
GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA 
GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA 
GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG 
GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC 
ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG 
AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG 
CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT 
GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA 
AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC 
TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG 
CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC 
CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG 
ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA 
GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG 
GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT 
GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA 
ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC 
ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC 
CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC 
AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT 
ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT 
ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT 
GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT 
GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC 
TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG 
CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA 
GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA 
CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT 
TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG 
AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT 
TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC 
AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT 
CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT 
GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA 
ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT 
AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA 
AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC 
ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT 
AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC 
AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG 
CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG 
GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG 
ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT 
GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA 
CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC 
ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT 
CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA 
TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT 
CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT 
CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC 
TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC 
CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT 
GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT 
GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA 
CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA 
GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG 
ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT 
TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA 
GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005912.1
CDS	26..4564
Translation	MAAAAGNRASSSGFPGARATSPEAGGGGGALKASSAPAAAAGLLREAGSGGRERADWRRRQLRKVRSVELDQLPEQPLFLAASPPASSTSPSPEPADAAGSGTGFQPVAVPPPHGAASRGGAHLTESVAAPDSGASSPAAAEPGEKRAPAAEPSPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPVKGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQTSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	NM_005921.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005921.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC 
GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT 
GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG 
AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC 
GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG 
GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA 
GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA 
GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG 
GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC 
ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG 
AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG 
CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT 
GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA 
AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC 
TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG 
CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC 
CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG 
ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA 
GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG 
GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT 
GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA 
ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC 
ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC 
CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC 
AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT 
ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT 
ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT 
GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT 
GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC 
TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG 
CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA 
GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA 
CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT 
TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG 
AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT 
TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC 
AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT 
CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT 
GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA 
ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT 
AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA 
AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC 
ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT 
AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC 
AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG 
CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG 
GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG 
ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT 
GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA 
CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC 
ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT 
CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA 
TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT 
CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT 
CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC 
TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC 
CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT 
GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT 
GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA 
CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA 
GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG 
ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT 
TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA 
GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Variation in MAP3K1 Associates with Ventilator-Free Days in Acute Respiratory Distress Syndrome.
American journal of respiratory cell and molecular biology58(1)117-125(2018 Jan)
Morrell ED,O'Mahony DS,Glavan BJ,Harju-Baker S,Nguyen C,Gunderson S,Abrahamson A,Radella F 2nd,Rona G,Black RA,Wurfel MM

MAP3K1-related gonadal dysgenesis: Six new cases and review of the literature.
American journal of medical genetics. Part C, Seminars in medical genetics175(2)253-259(2017 Jun)
Granados A,Alaniz VI,Mohnach L,Barseghyan H,Vilain E,Ostrer H,Quint EH,Chen M,Keegan CE

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MAP3K1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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