MED12 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12
Entrez Gene ID	422210
Full Name	mediator complex subunit 12
General protein information	Preferred Names mediator complex subunit 12 Names mediator of RNA polymerase II transcription subunit 12 mediator of RNA polymerase II transcription, subunit 12 homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015278410.1	XP_015133896.1	mediator of RNA polymerase II transcription subunit 12 isoform X2
XM_015278411.1	XP_015133897.1	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_015278409.2	XP_015133895.2	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_015278411.2	XP_015133897.2	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_025150336.1	XP_025006104.1	mediator of RNA polymerase II transcription subunit 12 isoform X4
XM_015278409.1	XP_015133895.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_015278410.2	XP_015133896.2	mediator of RNA polymerase II transcription subunit 12 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12	NP_005111.2
	MED12	XP_001088424.2
Rattus norvegicus (Norway rat)	Med12	NP_001180221.1
Danio rerio (zebrafish)	med12	NP_001034550.1
Pan troglodytes (chimpanzee)	MED12	NP_001009019.1
Bos taurus (cattle)	MED12	NP_001192807.1
Xenopus tropicalis (tropical clawed frog)	med12	XP_002934949.2
Gallus gallus (chicken)	MED12	XP_004940691.1
Mus musculus (house mouse)	Med12	NP_067496.2
Canis lupus familiaris (dog)	MED12	XP_003640267.1

Gene Ontology

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa150593	XM_015278409.2 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa150594	XM_015278410.2 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa150595	XM_015278411.2 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa150596	XM_025150336.1 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa34699	XM_015278409.1 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa34700	XM_015278410.1 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa34701	XM_015278411.1 Latest version!	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12 ( XM_015278409.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133895.2 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

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CloneID	OGa150593
Clone ID Related Accession (Same CDS sequence)	XM_015278409.2
Accession Version	XM_015278409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAGC AACAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCAGCC ACCACCACCG CAGCCACAGC CCCAGCAGCA GCCCCAGGTC 
TCCACAGTGC CTCAGCCACA GGCGCAGGGC CAGCCCCCAG GGCTGGGGAT GCAGGCTCTT 
CCCCCCCAGC AGCCCATTTT CCAGCGCCAG GGCCTTCAGC AGACGCAGCA GCAGCAGCAA 
ACAGCTGCTC TGGTTCGACA GCTTCAACAG CAGCTTTCCA ATACGCAACC ACAGCAGAAC 
AACAACCCCT TTGGGCGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133895.2
CDS	64..6567
Translation	MAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278409.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278409.2

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAGC AACAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCAGCC ACCACCACCG CAGCCACAGC CCCAGCAGCA GCCCCAGGTC 
TCCACAGTGC CTCAGCCACA GGCGCAGGGC CAGCCCCCAG GGCTGGGGAT GCAGGCTCTT 
CCCCCCCAGC AGCCCATTTT CCAGCGCCAG GGCCTTCAGC AGACGCAGCA GCAGCAGCAA 
ACAGCTGCTC TGGTTCGACA GCTTCAACAG CAGCTTTCCA ATACGCAACC ACAGCAGAAC 
AACAACCCCT TTGGGCGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_015278410.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133896.2 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

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CloneID	OGa150594
Clone ID Related Accession (Same CDS sequence)	XM_015278410.2
Accession Version	XM_015278410.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133896.2
CDS	64..6564
Translation	MAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278410.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278410.2

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_015278411.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133897.2 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

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CloneID	OGa150595
Clone ID Related Accession (Same CDS sequence)	XM_015278411.2
Accession Version	XM_015278411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGGGCCAA GGCATAATGG GACAGCAGAC AGTACGCCAG 
ATGGCTCCAA CCCCATCCTA TGGGGCACTA CAGCCCTCCC AGGGTTACAC ACCTTATGTC 
TCCCACATAG GGCTTCAGCA ACACCCTTCC CAGTCAGGCA CGATGGTGCC TCCTACATAT 
TCTGGCCAGC CCTATCAGAA TTCCCACCCC AGCTCTAATC CTGCCCTGGT GGATCCCGTC 
AGACAGATGC AGCAGAGACC AAGTGGCTAT GTGCACCAGC AGGCCCCAGG CTATGGGCAC 
ACCTTGGGCA ACACACAGAG ATTTCCTCAC CAGTCAATAC AGCAGGCTCC CATGATGACT 
GGGATGAACC ATTTGGGTCC CCAAGGAGTC CCCTCAGGAA TTCGACCCAG CCAGATACTG 
CCTGACCAGC AGCAGCAGCA GTACCTGCGG CAGCAGCAGC AACAGCAGCA GCAGATGCTG 
AGGCAGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133897.2
CDS	64..6564
Translation	MAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278411.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278411.2

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGGGCCAA GGCATAATGG GACAGCAGAC AGTACGCCAG 
ATGGCTCCAA CCCCATCCTA TGGGGCACTA CAGCCCTCCC AGGGTTACAC ACCTTATGTC 
TCCCACATAG GGCTTCAGCA ACACCCTTCC CAGTCAGGCA CGATGGTGCC TCCTACATAT 
TCTGGCCAGC CCTATCAGAA TTCCCACCCC AGCTCTAATC CTGCCCTGGT GGATCCCGTC 
AGACAGATGC AGCAGAGACC AAGTGGCTAT GTGCACCAGC AGGCCCCAGG CTATGGGCAC 
ACCTTGGGCA ACACACAGAG ATTTCCTCAC CAGTCAATAC AGCAGGCTCC CATGATGACT 
GGGATGAACC ATTTGGGTCC CCAAGGAGTC CCCTCAGGAA TTCGACCCAG CCAGATACTG 
CCTGACCAGC AGCAGCAGCA GTACCTGCGG CAGCAGCAGC AACAGCAGCA GCAGATGCTG 
AGGCAGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_025150336.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006104.1 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

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CloneID	OGa150596
Clone ID Related Accession (Same CDS sequence)	XM_025150336.1
Accession Version	XM_025150336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGGGAGCAT GTTTATTCAG CTTCCAGTCC TGAGAAGAAA 
CAGAGCCAGG ATGAAATTTG GAGATGCTTG CTGCCAGCAG GAGACGATGT TACTAGCCAG 
AGAAAGATGA ATTTGGGAAG TATGAAACAA ATGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006104.1
CDS	64..6222
Translation	MAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQREHVYSASSPEKKQSQDEIWRCLLPAGDDVTSQRKMNLGSMKQMT

Target ORF information:

RefSeq Version	XM_025150336.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150336.1

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTACGAG CACCGCCCGC TCAAGCGCCC GCGCCTCGGC 
CCGCCCGACG TGTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGGGAGCAT GTTTATTCAG CTTCCAGTCC TGAGAAGAAA 
CAGAGCCAGG ATGAAATTTG GAGATGCTTG CTGCCAGCAG GAGACGATGT TACTAGCCAG 
AGAAAGATGA ATTTGGGAAG TATGAAACAA ATGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_015278409.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133895.1 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

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CloneID	OGa34699
Clone ID Related Accession (Same CDS sequence)	XM_015278409.1
Accession Version	XM_015278409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAGC AACAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCAGCC ACCACCACCG CAGCCACAGC CCCAGCAGCA GCCCCAGGTC 
TCCACAGTGC CTCAGCCACA GGCGCAGGGC CAGCCCCCAG GGCTGGGGAT GCAGGCTCTT 
CCCCCCCAGC AGCCCATTTT CCAGCGCCAG GGCCTTCAGC AGACGCAGCA GCAGCAGCAA 
ACAGCTGCTC TGGTTCGACA GCTTCAACAG CAGCTTTCCA ATACGCAACC ACAGCAGAAC 
AACAACCCCT TTGGGCGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133895.1
CDS	52..6555
Translation	MAAFGVLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278409.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278409.1

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAGC AACAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCAGCC ACCACCACCG CAGCCACAGC CCCAGCAGCA GCCCCAGGTC 
TCCACAGTGC CTCAGCCACA GGCGCAGGGC CAGCCCCCAG GGCTGGGGAT GCAGGCTCTT 
CCCCCCCAGC AGCCCATTTT CCAGCGCCAG GGCCTTCAGC AGACGCAGCA GCAGCAGCAA 
ACAGCTGCTC TGGTTCGACA GCTTCAACAG CAGCTTTCCA ATACGCAACC ACAGCAGAAC 
AACAACCCCT TTGGGCGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_015278410.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133896.1 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

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CloneID	OGa34700
Clone ID Related Accession (Same CDS sequence)	XM_015278410.1
Accession Version	XM_015278410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133896.1
CDS	52..6552
Translation	MAAFGVLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278410.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278410.1

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGCAGGGC CAAGGCATAA TGGGACAGCA GACAGTACGC 
CAGATGGCTC CAACCCCATC CTATGGGGCA CTACAGCCCT CCCAGGGTTA CACACCTTAT 
GTCTCCCACA TAGGGCTTCA GCAACACCCT TCCCAGTCAG GCACGATGGT GCCTCCTACA 
TATTCTGGCC AGCCCTATCA GAATTCCCAC CCCAGCTCTA ATCCTGCCCT GGTGGATCCC 
GTCAGACAGA TGCAGCAGAG ACCAAGTGGC TATGTGCACC AGCAGGCCCC AGGCTATGGG 
CACACCTTGG GCAACACACA GAGATTTCCT CACCAGTCAA TACAGCAGGC TCCCATGATG 
ACTGGGATGA ACCATTTGGG TCCCCAAGGA GTCCCCTCAG GAATTCGACC CAGCCAGATA 
CTGCCTGACC AGCAGCAGCA GCAGTACCTG CGGCAGCAGC AGCAACAGCA GCAGCAGATG 
CTGAGGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_015278411.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133897.1 Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

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CloneID	OGa34701
Clone ID Related Accession (Same CDS sequence)	XM_015278411.1
Accession Version	XM_015278411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGGGCCAA GGCATAATGG GACAGCAGAC AGTACGCCAG 
ATGGCTCCAA CCCCATCCTA TGGGGCACTA CAGCCCTCCC AGGGTTACAC ACCTTATGTC 
TCCCACATAG GGCTTCAGCA ACACCCTTCC CAGTCAGGCA CGATGGTGCC TCCTACATAT 
TCTGGCCAGC CCTATCAGAA TTCCCACCCC AGCTCTAATC CTGCCCTGGT GGATCCCGTC 
AGACAGATGC AGCAGAGACC AAGTGGCTAT GTGCACCAGC AGGCCCCAGG CTATGGGCAC 
ACCTTGGGCA ACACACAGAG ATTTCCTCAC CAGTCAATAC AGCAGGCTCC CATGATGACT 
GGGATGAACC ATTTGGGTCC CCAAGGAGTC CCCTCAGGAA TTCGACCCAG CCAGATACTG 
CCTGACCAGC AGCAGCAGCA GTACCTGCGG CAGCAGCAGC AACAGCAGCA GCAGATGCTG 
AGGCAGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133897.1
CDS	52..6552
Translation	MAAFGVLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGQSCGSPAGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSTQTGNALPSTPTPQPATGNPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGNSGIKLEDTGLSEPMDIDHNSSTIFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSAEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTANNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASATPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRRVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAVEPSKADKTSSNPATSTEERKKKQSKTKKRNQSASKSEDFVLGPSRGVSYGVGMPTDLLHHQAGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPSGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLGQGIMGQQTVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQAPMMTGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQPPPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_015278411.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278411.1

ORF Insert Sequence:

ATGGCTGCCT TCGGCGTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ATGAGCTGAC GGCCCTGAAC 
GTGAAGCAGG GCTTCAACAA CCAGCCCGCC GTCTCCGGGG ACGAGCACGG CAGCGCCAAG 
AACGTCAATT TCAATCCGGC CAAGATCAGT TCAAATTTCA GCAGTATTAT TGCAGAAAAG 
CTGCGGTGCA ATACGCTGCC AGACACAGGA AGAAGGAAAC CCCAGGTGAA TCAGAAGGAC 
AACTTCTGGC TGGTGACAGC ACGTTCACAG AGTGCCATAA ACAACTGGTT CACGGATCTG 
GCTGGAACAA AGCCCCTCAC TCACCTCGCC AAGAAGGTAC CCATCTTTAG CAAGAAGGAA 
GAGGTCTTTG GCTATTTGGC AAAATACACT GTTCCAGTAA TGAGAGCAGC CTGGCTCATC 
AAAATGACTT GTGCCTATTA TGCTGCCATC ACAGAAACCA AGGTGAAAAA GCGGCACGTC 
ATCGATCCCT TCATCGAATG GACACAGATC ATCACCAAGT ACCTGTCGGA ACAGCTGCAG 
AAGATCGCAG AGTTCTACAG ACAGCTCCCA GGGCAAAGCT GTGGGTCACC GGCCGGGCCA 
ATGCCCCAGG AGGTGGAACA AGCTTTGAAG CAGTGGGACT ACAACGAGAA ACTAGCCATG 
TTCATGTTCC AGGATGGCAT GCTGGACCGG CATGAATTCC TGACGTGGGT GCTTGAGTGC 
TTTGAGAAGA TACGATCAGG AGAGGATGAG TTTCTGAAAA TGCTCCTACC CCTCCTGCTG 
CGGTACTCTG GAGAGTTTGT GCAGTCTGCC TACCTGTCCA GACGTCTGGC CTACTTCTGC 
ACCCGCAGGC TTGCTATGCA GCTGGATGGT GCTGGTGGGC ACCCGCCCCA CATCCTGTCT 
ACGCAGACAG GGAATGCTCT TCCTTCAACT CCCACCCCTC AGCCTGCTAC AGGGAATCCT 
CCTCCCAGCC CTTTTAGCGA CTTACTGCTG TGCCCCCAGC ACCGACCAGT GGTGTATGGG 
CTCAGCTGCA TCCTCCAGAG TATAATTTTG TGTTGCCCAA GTGCCCTTGT GTGGCATTAC 
TCACTGACTG ACAGCAGGAT AAAGACTGGC TCTCCATTGG ACCACCTGCC TATAGCCCCA 
TCCAACTTGC CCATGCCAGG AGGGAATTCA GCCTTTACAC AGCAGGTTCG TGCAAAGCTC 
CGTGAAATTG AGCAGCAGAT AAAAGAACGT GGCCAAGCTG TGGAGGTTCG TTGGTCCTTT 
GACAAGTGCC AGGAAACCAC AGCAGGTTTC ACCATTGGCC GTGTCTTGCA CACTTTAGAA 
GTTCTGGACA GTCACAGCTT TGAAAGATCT GACTTCAGCA ACTCTTTGGA TTCCTTATAT 
AACAGAATAT TTGGGCTGGG TCCAACCAAG GACAGTCATG AGATCTCCCC AGATGACGAT 
GCAGTGGTGG CCTTGCTGTG TGAGTGGGCT GTCAGCTATA AACGCTCTGG GCGCCACAGG 
GCTATGGTTG TGGCCAAACT CCTGGAGAAG CGTCAAGCAG AGATAGAGGC TGAGAGATGT 
GGGGACTCCG AAGTTGTGGA TGAGAAAGGC TCCATCTCGT CAGGCTCTCT CTCAGCAGCC 
AGTGCTCCTG TCTTTCAGGA TGTTCTTATG CAGTTCCTTG ACACTCAAGC TCCTATGCTA 
ACTGATCCTG GGAAGGAAAA TGAGAAGGTG GAATTTTTTA ACCTGGTGCT GCTATTCTGT 
GAGCTAATCC GACATGATGT CTTCTCTCAC AACATCTACA TGTGCACACT CATCTCCCGG 
GGTGATCTCG CCATGGATTC TCATGGACCT CGTCCTCCCT CACCCTTTGA TGACCCTGCT 
GAGGAGCACG ACAGGAAGGA GACAGAAGGA AACAGTGGGA TCAAGCTAGA GGACACAGGT 
CTTTCTGAGC CCATGGACAT TGACCACAAT TCCAGCACAA TCTTTGATGA CATGGAGAAG 
ACAGATTTTT CGATGTTTTC TCCTCCAATG CATTGTGAAT CTAAAGCCAG CCCTTCGCCT 
GAGAAACCAG ATCCTGAAAA GGAAGCAAAG CCTCTACTGA AGGACAAGTC TGCAGAAGGA 
ATGCTAGCAT CTCTGTATGA CCAGCCTCGG CACATCCAGT ATGCAACACA CTTTCCTATT 
CCTCAGGAGG AATCGTGCAG TCACGAATGT AACCAACGGT TGGTAGTTCT CTTTGGGGTT 
GGAAAACAAC GGGATGATGC TCGGCATACA ATCAAGAAAA TAACCAAAGA CATCCTAAAA 
GTTTTAAACA GAAAAAGTAC TGCAGAGACA GGTGGAGAGG AAGGCCAGAA GAGGAAAAAG 
AGCAAGCCAG AAGCATTCCC AACAGCTGAA GATATCTTTG CAAAGTTCCA GCACCTTTCT 
CACTTTGATC AGCACCAAGT CACATCTCAG GTATCCCGCA ATGTCTTGGA ACAGATCACC 
AGCTTTGCCT TGGGCATGTC CTACCACCTG CCTCTGGTAC AGCATGTGCA GTTCATCTTT 
GACTTGATGG AGTATTCACT CAATATCAGT GGTCTTATCG ACTTTGCCAT CCAGTTGCTG 
AATGAACTGA GCGTGGTGGA GGCAGAACTG CTGTTGAAAT CCTCCGACCT TGTTGGCAGC 
TACACCACCA GCTTGTGCCT GTGCATCGTG GCTGTGCTGC GGCACTACCA TTCCTGCCTT 
ATACTGAACC AGGACCAGAT GGCTCAGGTC TTTGAAGGTC TGTGTGGGGT GGTGAAACAT 
GGCATGAACC GCTCGGATGG CTCCTCGGCA GAGCGCTGTA TCCTGGCCTA TCTCTATGAC 
CTGTATACCT CCTGCAGTCA CCTCAAAAGT AAATTTGGGG AGCTTTTTAG TGACTTCTGC 
TCCAAGGTGA AGAACACAAT CTACTGCAAC GTTGAGCCAT CTGATTCCAA CATGCTATGG 
GAACCAGAGT TCATGATTGA CACTATTGAA AATCCATCTG CACATAACTT CACATACACC 
AACCTGGGCA AGAGCCTAAA TGAAAACCCA GCAAACCGCT ACAGTTTTGT CTGTAATGCA 
CTTATGCATG TGTGTGTGGG GCACCATGAT CCAGACAGGG TGAATGACAT CGCCATCCTG 
TGTGCTGAAC TGACTGGTTA CTGCAAGTCT CTAAGCGCCG AGTGGCTGGG TGTGCTTAAA 
GCTTTGTGCT GTTCCTCCAA TAATGGGACC TGTGGTTTCA ATGATCTCCT CTGCAATGTT 
GATGTCAGTG ACTTATCTTT CCATGACTCC TTGGCCACCT TTGTTGCCAT TCTCATTGCC 
CGGCAGTGCT TGCTGCTGGA GGACCTCATT CGCTGTGCTG CTATCCCTTC ACTCCTCAAT 
GCTGCCTGCA GTGAACAGGA CTCTGAACCA GGAGCACGTT TGACCTGCCG GATTTTACTC 
CATCTGTTTA AGACTCCACA GCTGAACCCG TGCCAGCAAG ATGGCAACAA ACCCACGGTG 
GGAATCCGTT CTTCTTGTGA CCGTCACTTA CTGGCAGCTT CCCAGAATCG TATTGTGGAC 
GGAGCAGTCT TTGCAGTTCT GAAGGCTGTC TTTGTTCTGG GAGATGCAGA ACTGAAAGGC 
TCTGGCTTTT CCCACCCTGG AGGTGTCGAT GATCTGATGG ATGATGAGCT GGGTACCAGG 
AAGGCTGGTG GTCGGGTAGT AACTGTGGAA ACAGCTAGCC TGGATATTTA TGCCAAGTAT 
GTACTGAGGA GCATCTGCCA ACAGGAGTGG GTAGGTGAGC GATGTCTGAA GTCTCTCTGT 
GAAGACAGTA ATGACTTGCA GGATCCTGTC TTGAGCAGCA CACAGGCCCA GAGGCTGATG 
CAGCTCATCT GTTATCCACA CCGACTGCTG GACAATGAGG AAGGAGAAAA TCCTCAGCGG 
CAGAGGATTA AGCGCATCCT ACAGAATCTG GATCAGTGGA CCATGAGGCA GTCCTCGCTG 
GAGCTGCAGC TCATGATTAA GCAGACTGCA AATAATGAGA TGAACTCCTT GTTAGAAAAT 
ATAGCCAAGG CCACTATTGA GGTATTCCAG CAGTCAGCAG AGACCAGCTC TGCTAGTTCT 
GCTGGCAATG GAGTCAACAA TATCAGTAGC TCAGCGAGTG CTACCCCAGC CAGCAACAAA 
TCCAAACCCA TCCTCAGTTC TCTGGAGAGA TCAGGAGTGT GGCTGGTGGC CCCTCTGATT 
GCCAAGCTCC CAACATCAGT GCAGGGCCAC GTGCTGAAAG CTGCTGGAGA AGAACTGGAG 
AAAGGACAAC ATTTGGGGTC ATCATCCCGC AAAGAGCGGG ACCGCCAGAA GCAGAAGAGC 
ATGTCCCTGC TGAGCCAGCA GCCATTTCTC TCGCTGGTGC TGACTTGCTT GAAGGGGCAG 
GATGAGCAGC GGGAGGGTCT CCTCACCTCT CTGTACAGTC AGGTCCAGCA GATTGTCACG 
AATTGGCGAG AAGATCAATA CCAAGATGAC TGCAAAGCCA AGCAGCTGAT GCATGAGGCC 
CTGAAGTTAC GACTGAATTT GGTGGGAGGG ATGTTTGACA CAGTTCAACG CAGTACACAG 
CAGACAACTG AATGGGCTGT GCTTCTCCTG GACATCATCA GCAGTGGCAC TGTGGACATG 
CAGTCAAACA ATGAGCTCTT CACCACTGTG TTGGACATGC TGAGTGTTCT CATCAACGGC 
ACCCTGGCTG CTGATATGTC CAGCATTTCT CAGGGCAGCA TGGAGGAGAA CAAGCGGGCA 
TACATGAATC TTGTCAAGAA ACTCAGGAAG GAGCTGGGGG ACCGGCAGTC TGACAGCCTG 
GAGAAGGTGC GACAGCTGCT GCCACTGCCC AAGCAGACCC GGGATGTCAT CACCTGCGAA 
CCTCAGGGAT CCCTCATTGA CACCAAAGGC AATAAAATAG CTGGTTTTGA CTCCATCTTC 
AAGAAGGAGG GTTTGCAAGT CTCTACGAAG CAGAAGATTT CCCCTTGGGA TCTGTTTGAG 
GGCTTAAAGC ACTCAGCCCC CCTCTCCTGG GGATGGTTTG GGACAGTCCG GGTGGACCGC 
AGGGTGTCTA GATTTGAGGA GCAGCAGAGG CTTCTTCTGT ACCACACACA CTTAAAACCC 
AAGCCTCGCA GTTACTACCT GGAGCCATTG CCTCTGCCCC CCGAAGAGGA GGAGCCTCCT 
ACACCTGTGG CTTTAGAGCC AGAGAAGAAG GCTGTGGAAC CAAGCAAGGC TGATAAAACA 
AGCTCCAATC CTGCCACTTC TACTGAGGAA CGCAAGAAGA AACAGAGCAA AACCAAGAAA 
CGCAACCAGT CTGCCAGCAA AAGTGAGGAT TTTGTGTTGG GTCCCAGCCG AGGGGTTTCA 
TATGGAGTTG GCATGCCTAC GGATCTCTTG CATCACCAAG CAGGAAGCAC TATGTCTAGG 
CTGGCATATG GACAGTCACC AGTGGGTCTC TATGCCCAGA ATCAACCTCT TCCAGCAGGT 
GGCCCTCGCC TGGATACATC CTACAGACCC GTGCGCATGC CGTTGGGAAA ACTTGTTCAG 
AGTCGTCCTC CCTACAGTGG GGTTCTGCCT TCAGGGATGG GGAGCATGAT GGGCATTGAC 
CCCTCCTACA AGCCAGCAGT GTACAGACAG CAGCCTCCGG TGTCCCAGGG ACAGATATTG 
AGGCAGCAAC TTCAAGCAAA GTTGGGCCAA GGCATAATGG GACAGCAGAC AGTACGCCAG 
ATGGCTCCAA CCCCATCCTA TGGGGCACTA CAGCCCTCCC AGGGTTACAC ACCTTATGTC 
TCCCACATAG GGCTTCAGCA ACACCCTTCC CAGTCAGGCA CGATGGTGCC TCCTACATAT 
TCTGGCCAGC CCTATCAGAA TTCCCACCCC AGCTCTAATC CTGCCCTGGT GGATCCCGTC 
AGACAGATGC AGCAGAGACC AAGTGGCTAT GTGCACCAGC AGGCCCCAGG CTATGGGCAC 
ACCTTGGGCA ACACACAGAG ATTTCCTCAC CAGTCAATAC AGCAGGCTCC CATGATGACT 
GGGATGAACC ATTTGGGTCC CCAAGGAGTC CCCTCAGGAA TTCGACCCAG CCAGATACTG 
CCTGACCAGC AGCAGCAGCA GTACCTGCGG CAGCAGCAGC AACAGCAGCA GCAGATGCTG 
AGGCAGCAAC AGCAGCAGCA GCAACAACAG CAGCAGCAGC AACAGCAACA ACAGCAACAG 
CAGCAGCAGC AGCAGCCACC ACCACCGCAG CCACAGCCCC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCAGGGC TGGGGATGCA GGCTCTTCCC 
CCCCAGCAGC CCATTTTCCA GCGCCAGGGC CTTCAGCAGA CGCAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CGCAACCACA GCAGAACAAC 
AACCCCTTTG GGCGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MED12 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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