MED12 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12
Entrez Gene ID	449626
Full Name	mediator complex subunit 12
Synonyms	TNRC11
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016942621.1	XP_016798110.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_016942622.1	XP_016798111.1	mediator of RNA polymerase II transcription subunit 12 isoform X2
XM_016942622.2	XP_016798111.1	mediator of RNA polymerase II transcription subunit 12 isoform X2
XM_016942623.1	XP_016798112.1	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_016942621.2	XP_016798110.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
NM_001009019.1	NP_001009019.1	mediator of RNA polymerase II transcription subunit 12
XM_016942623.2	XP_016798112.1	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_024352956.1	XP_024208724.1	mediator of RNA polymerase II transcription subunit 12 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12	NP_005111.2
	MED12	XP_001088424.2
Rattus norvegicus (Norway rat)	Med12	NP_001180221.1
Danio rerio (zebrafish)	med12	NP_001034550.1
Pan troglodytes (chimpanzee)	MED12	NP_001009019.1
Bos taurus (cattle)	MED12	NP_001192807.1
Xenopus tropicalis (tropical clawed frog)	med12	XP_002934949.2
Gallus gallus (chicken)	MED12	XP_004940691.1
Mus musculus (house mouse)	Med12	NP_067496.2
Canis lupus familiaris (dog)	MED12	XP_003640267.1

Gene Ontology
Bibliography

Related articles in PubMed

Major chimpanzee-specific structural changes in sperm development-associated genes.
Kim RN, Kim DW, Choi SH, Chae SH, Nam SH, Kim DW, Kim A, Kang A, Park KH, Lee YS, Hirai M, Suzuki Y, Sugano S, Hashimoto K, Kim DS, Park HS
Functional & integrative genomics11(3)507-17(2011 Sep)

Gene diversity patterns at 10 X-chromosomal loci in humans and chimpanzees.
Kitano T, Schwarz C, Nickel B, P??bo S
Molecular biology and evolution20(8)1281-9(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc110102	XM_024352956.1 Latest version!	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc00112	NM_001009019.1 Latest version!	Pan troglodytes mediator complex subunit 12 (MED12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPc90093	XM_016942621.2 Latest version!	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPc90094	XM_016942622.2 Latest version!	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPc90095	XM_016942623.2 Latest version!	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OPc90094	XM_016942622.1	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPc90093	XM_016942621.1	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPc90095	XM_016942623.1	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12 ( XM_024352956.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024208724.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc110102
Clone ID Related Accession (Same CDS sequence)	XM_024352956.1
Accession Version	XM_024352956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GATCCTACCC GCCACCTGCA ACAGCGGCCC AGTGGCTATG TGCACCAGCA GGCCCCCACC 
TATGGACATG GACTGACCTC CACTCAAAGG TTTTCACACC AGACACTGCA GCAGACACCC 
ATGATAAGTA CCATGACTCC AATGAGTGCC CAGGGCGTCC AGGCAGGCGT CCGCTCAACA 
GCCATCCTAC CTGAGCAGCA GCAGCAGCAG CAACAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAG CAGTACCACA TCCGGCAGCA GCAGCAGCAG 
CAGATCCTGC GGCAGCAGCA GCAACAGCAA CAGCAGCAGC AGCAGCAGCA GCAACAGCAA 
CAGCAGCAGC AGCAACAGCA ACAACAGCAA CACCAGCAGC AACAGCAGCA ACAGGCGGCT 
CCTCCCCAAC CCCAGCCCCA GTCCCAGCCC CAGTTCCAGC GCCAGGGGCT TCAGCAGACC 
CAGCAGCAGC AACAGACAGC AGCTTTGGTC CGGCAACTTC AACAACAGCT CTCTAATACC 
CAGCCACAGC CCAGTACCAA CATATTTGGA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024208724.1
CDS	115..6573
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_024352956.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024352956.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GATCCTACCC GCCACCTGCA ACAGCGGCCC AGTGGCTATG TGCACCAGCA GGCCCCCACC 
TATGGACATG GACTGACCTC CACTCAAAGG TTTTCACACC AGACACTGCA GCAGACACCC 
ATGATAAGTA CCATGACTCC AATGAGTGCC CAGGGCGTCC AGGCAGGCGT CCGCTCAACA 
GCCATCCTAC CTGAGCAGCA GCAGCAGCAG CAACAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAG CAGTACCACA TCCGGCAGCA GCAGCAGCAG 
CAGATCCTGC GGCAGCAGCA GCAACAGCAA CAGCAGCAGC AGCAGCAGCA GCAACAGCAA 
CAGCAGCAGC AGCAACAGCA ACAACAGCAA CACCAGCAGC AACAGCAGCA ACAGGCGGCT 
CCTCCCCAAC CCCAGCCCCA GTCCCAGCCC CAGTTCCAGC GCCAGGGGCT TCAGCAGACC 
CAGCAGCAGC AACAGACAGC AGCTTTGGTC CGGCAACTTC AACAACAGCT CTCTAATACC 
CAGCCACAGC CCAGTACCAA CATATTTGGA CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( NM_001009019.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> NP_001009019.1 Pan troglodytes mediator complex subunit 12 (MED12), mRNA.

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CloneID	OPc00112
Clone ID Related Accession (Same CDS sequence)	NM_001009019.1
Accession Version	NM_001009019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB102669.1. On Jan 6, 2005 this sequence version replaced XM_521116.1. ##Evidence-Data-START## Transcript exon combination :: AB102669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03018695 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGCTG CCTGGCTCAT TAAGATGACC TGTGCCTACT ATGCAGCAAT CTCTGAGACC 
AAGGTTAAGA AGAGACATGT TGACCCTTTC ATGGAATGGA CTCAGATCAT CACCAAGTAC 
TTATGGGAGC AGTTACAGAA GATGGCTGAA TACTACCGGC CAGGGCCTGC AGGAAGTGGG 
GGCTGTGGTT CCACGATAGG GCCCTTGCCC CATGATGTAG AGGTGGCAAT CCGGCAGTGG 
GATTACACCG AGAAGCTGGC CATGTTCATG TTTCAGGATG GAATGCTGGA CAGACATGAG 
TTCCTGACCT GGGTGCTTGA GTGTTTTGAG AAGATCCGCC CTGGAGAGGA TGAATTGCTT 
AAACTGCTGC TGCCTCTGCT TCTCCGATAC TCTGGGGAAT TTGTTCAGTC TGCATACCTG 
TCCCGCCGGC TTGCCTACTT CTGTACACGG AGACTGGCCC TGCAGCTGGA TGGTGTGAGC 
AGTCACTCAT CTCATGTTAT ATCTGCTCAG TCAACAAGCA CGCTACCCAC CACCCCTGCT 
CCTCAGCCCC CAACTAGCAG CACACCCTCG ACTCCCTTTA GTGACCTGCT TATGTGCCCT 
CAGCACCGGC CCCTGGTTTT TGGCCTCAGC TGTATCCTAC AGACCATCCT CCTGTGCTGT 
CCTAGTGCCT TGGTTTGGCA CTACTCACTG ACTGATAGCA GAATTAAGAC CGGCTCACCA 
CTTGACCACT TGCCTATTGC CCCGTCCAAC CTGCCCATGC CAGAGGGTAA CAGTGCCTTC 
ACTCAGCAGG TCCGTGCAAA GTTGCGGGAG ATCGAGCAGC AGATCAAGGA GCGGGGACAG 
GCAGTTGAAG TTCGCTGGTC TTTCGATAAA TGCCAGGAAG CTACTGCAGG CTTCACCATT 
GGACGGGTAC TTCATACTTT GGAAGTGCTG GACAGCCATA GTTTTGAGCG CTCTGACTTC 
AGCAACTCTC TTGACTCCCT TTGTAACCGA ATCTTTGGAT TGGGGCCTAG CAAGGATGGG 
CATGAGATCT CCTCAGATGA TGATGCTGTG GTGTCATTGC TATGTGAATG GGCTGTCAGC 
TGCAAGCGTT CTGGTCGGCA TCGTGCTATG GTGGTAGCCA AGCTCCTGGA GAAGAGACAG 
GCGGAGATTG AGGCTGAGCG TTGTGGAGAA TCAGAAGCCG CAGATGAGAA GGGTTCCATC 
GCCTCTGGCT CCCTTTCTGC TCCCAGTGCT CCCATTTTCC AGGATGTCCT CCTGCAGTTT 
CTGGATACAC AGGCTCCCAT GCTGACGGAC CCTCGAAGTG AGAATGAGCG GGTGGAATTC 
TTTAACTTAG TACTGCTGTT CTGTGAACTG ATTCGACATG ATGTTTTCTC CCACAACATG 
TATACTTGCA CTCTCATCTC CCGAGGGGAC CTTGCCTTTG GAGCCCCTGG TCCCCGGCCT 
CCCTCTCCCT TTGATGATCC TGCCGATGAC GCAGAGCACA AGGAGGCTGA AGGCAGCAGC 
AGCAGCAAGC TGGAAGATCC AGGGCTCTCA GAATCTATGG ACATTGACCC TAGTTCCAGT 
GTGCTCTTTG AGGACATGGA GAAGCCTGAT TTCTCATTGT TCTCCCCTAC TATGCCCTGT 
GAGGGGAAGG GCAGTCCATC CCCTGAGAAG CCAGATGTCG AGAAGGAGGT GAAGCCCCCA 
CCCAAGGAGA AGATTGAAGG GACCCTTGGG ATTCTTTATG ACCAGCCACG ACACGTGCAG 
TACGCCACCC ATTTTCCCAT CCCCCAGGAG GAGTCATGCA GCCATGAGTG CAACCAGCGG 
TTGGTCGTGC TGTTTGGGGT GGGAAAGCAG CGAGATGATG CCCGCCATGC CATCAAGAAA 
ATCACCAAGG ATATCTTGAA GGTTCTGAAC CGCAAAGGGA CAGCAGAAAC TGACCAGCTT 
GCTCCTATTG TGCCTCTGAA TCCTGGAGAC CTGACATTCT TAGGTGGGGA GGATGGGCAG 
AAGCGGCGAC GCAACCGGCC TGAAGCCTTC CCCACTGCTG AAGATATCTT TGCTAAGTTC 
CAGCACCTTT CACATTATGA CCAACACCAG GTCACGGCTC AGGTCTCCCG GAATGTTCTG 
GAGCAGATCA CGAGCTTTGC CCTTGGCATG TCATACCACT TGCCTCTGGT GCAGCATGTG 
CAGTTCATCT TCGACCTCAT GGAATATTCA CTCAGCATCA GTGGCCTCAT CGACTTTGCC 
ATTCAGCTGC TGAATGAACT GAGTGTAGTT GAGGCTGAGC TGCTTCTCAA ATCCTCGGAT 
CTGGTGGGCA GCTACACTAC TAGCCTGTGC CTGTGCATCG TGGCTGTCCT GCGGCACTAT 
CATGCCTGCC TCATCCTCAA CCAGGACCAG ATGGCACAGG TCTTTGAGGG GCTGTGTGGC 
GTCGTGAAGC ATGGGATGAA CCGGTCCGAT GGCTCCTCTG CAGAGCGCTG TATCCTTGCT 
TATCTCTATG ATCTGTACAC CTCTTGTAGC CATTTAAAGA ACAAATTTGG GGAGCTCTTC 
AGCGACTTTT GCTCAAAGGT GAAGAACACC ATCTACTGCA ACGTGGAGCC ATCGGAATCA 
AATATGCGCT GGGCACCTGA GTTCATGATC GACACTCTAG AGAACCCTGC AGCTCACACC 
TTCACCTACA CGGGGCTAGG CAAGAGTCTT AGTGAGAACC CTGCTAACCG CTACAGCTTT 
GTCTGCAATG CCCTTATGCA CGTCTGTGTG GGGCACCATG ATCCCGATAG GGTGAATGAC 
ATCGCAATCC TGTGTGCAGA GCTGACCGGC TATTGCAAGT CACTGAGTGC AGAATGGCTA 
GGAGTGCTTA AGGCCTTGTG CTGCTCCTCT AACAATGGCA CTTGTGGTTT CAACGATCTC 
CTCTGCAATG TTGATGTCAG TGACCTGTCT TTTCATGACT CGCTGGCTAC TTTTGTTGCC 
ATCCTCATCG CTCGGCAGTG TTTGCTCCTG GAAGATCTGA TTCGCTGTGC TGCCATCCCT 
TCACTCCTTA ATGCTGCTTG TAGTGAACAG GACTCTGAGC CAGGGGCCCG GCTTACCTGC 
CGCATCCTCC TTCACCTTTT CAAGACACCG CAGCTCAATC CTTGCCAGTC TGATGGAAAC 
AAGCCTACAG TAGGAATCCG CTCCTCCTGC GACCGCCACC TGCTGGCTGC CTCCCAGAAC 
CGCATCGTGG ATGGAGCCGT GTTTGCTGTT CTGAAGGCTG TGTTTGTACT TGGGGATGCG 
GAACTGAAAG GTTCAGGCTT CACTGTGACA GGAGGAACAG AAGAACTTCC AGAGGAGGAG 
GGAGGAGGTG GCAGTGGTGG TCGGAGGCAG GGTGGCCGCA ACATCTCTGT GGAGACAGCC 
AGTCTGGACG TCTATGCCAA GTACGTGCTG CGCAGCATCT GCCAACAGGA ATGGGTAGGA 
GAACGTTGCC TTAAGTCTCT GTGTGAGGAC AGCAATGACC TGCAAGACCC AGTGTTGAGT 
AGTGCCCAGG CGCAGCGCCT CATGCAGCTC ATTTGCTATC CACATCGACT GCTGGACAAT 
GAGGATGGGG AAAACCCCCA GCGGCAGCGC ATAAAGCGCA TTCTCCAGAA CTTGGACCAG 
TGGACCATGC GCCAGTCTTC CTTGGAGCTG CAGCTCATGA TCAAGCAGAC CCCTAACAAT 
GAGATGAACT CCCTCTTGGA GAACATCGCC AAGGCCACAA TCGAGGTTTT CCAACAGTCA 
GCAGAGACAG GGTCATCTTC TGGAAGTACT GCAAGCAACA TGCCCAGCAG CAGCAAGACC 
AAGCCTGTGC TCAGCTCTCT AGAGCGCTCT GGTGTATGGC TGGTGGCCCC CCTTATTGCT 
AAACTGCCCA CCTCAGTCCA GGGACATGTG TTAAAGGCTG CTGGGGAAGA ATTGGAGAAG 
GGTCAGCACC TGGGTTCCTC TTCACGCAAA GAACGTGATC GACAAAAGCA GAAGAGCATG 
TCCCTATTGA GCCAGCAGCC CTTCTTATCG CTGGTGCTAA CATGTCTGAA AGGGCAGGAT 
GAACAACGCG AGGGACTCCT TACCTCCCTC TACAGCCAGG TGCACCAGAT TGTGAATAAT 
TGGCGAGATG ACCAGTACTT AGATGATTGC AAACCAAAGC AGCTTATGCA TGAGGCACTC 
AAACTGCGGC TCAACCTGGT GGGGGGCATG TTTGACACGG TGCAGCGCAG CACCCAGCAG 
ACCACGGAGT GGGCCATGCT CCTCCTGGAG ATCATCATCA GCGGCACTGT CGACATGCAG 
TCCAACAATG AGCTCTTCAC TACTGTGTTG GACATGCTGA GCGTGCTCAT CAATGGGACA 
TTGGCTGCAG ACATGTCTAG CATCTCACAA GGTAGCATGG AGGAAAACAA GCGTGCATAC 
ATGAACCTGG CGAAGAAGTT GCAGAAGGAG TTGGGGGAGC GCCAGTCGGA CAGTCTGGAA 
AAGGTTCGCC AGCTGCTGCC ACTGCCCAAG CAGACCCGAG ATGTCATCAC GTGTGAGCCA 
CAGGGCTCCC TTATCGATAC CAAGGGCAAC AAGATTGCTG GCTTCGATTC CATCTTCAAG 
AAGGAGGGTC TACAGGTTTC CACCAAACAG AAGATCTCGC CCTGGGATCT TTTCGAGGGG 
TTGAAGCCGT CAGCACCACT CTCTTGGGGC TGGTTTGGAA CAGTCCGAGT GGACCGGCGA 
GTGGCTCGAG GAGAGGAGCA GCAGCGGTTG CTGCTCTACC ACACACACCT GAGGCCCCGG 
CCCCGCGCCT ATTACCTGGA GCCACTGCCA CTGCCCCCAG AAGATGAGGA GCCGCCTGCT 
CCTACCCTGC TAGAGCCTGA GAAAAAGGCT CCAGAGCCCC CCAAAACTGA CAAACCGGGG 
GCTGCTCCAC CCAGTACTGA GGAACGCAAG AAGAAGTCCA CCAAGGGCAA GAAACGCAGC 
CAGCCAGCTA CCAAGACAGA GGACTATGGA ATGGGCCCGG GTCGGAGCGG CCCTTATGGT 
GTGACAGTGC CTCCGGACCT CCTGCACCAC CCAAACCCTG GTTCTATAAC ACACCTTAAC 
TATAGGCAAG GCTCCATAGG CCTGTACACC CAGAACCAGC CACTACCTGC AGGTGGCCCT 
CGTGTGGACC CATACCGCCC TGTGCGCTTA CCAATGCAGA AGCTGCCCAC CCGACCGACT 
TACCCTGGAG TGCTGCCCAC AACCATGACT GGCGTCATGG GTTTAGAACC CTCCTCTTAT 
AAGACCTCTG TGTACCGGCA GCAGCAACCT GCGGTGCCCC AAGGACAGCG CCTTCGCCAA 
CAGCTCCAGG CAAAGATACA GAGTCAGGGC ATGTTGGGAC AGTCATCTGT CCATCAGATG 
ACTCCCAGCT CTTCCTACGG TTTGCAGACT TCCCAGGGCT ATACTCCTTA TGTTTCTCAT 
GTGGGATTGC AGCAACACAC AGGCCCTGCA GGTACCATGG TGCCCCCCAG CTACTCCAGC 
CAGCCTTACC AGAGCACCCA CCCTTCTACC AATCCTACTC TTGTAGATCC TACCCGCCAC 
CTGCAACAGC GGCCCAGTGG CTATGTGCAC CAGCAGGCCC CCACCTATGG ACATGGACTG 
ACCTCCACTC AAAGGTTTTC ACACCAGACA CTGCAGCAGA CACCCATGAT AAGTACCATG 
ACTCCAATGA GTGCCCAGGG CGTCCAGGCA GGCGTCCGCT CAACAGCCAT CCTACCTGAG 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAACAGCAGC AGCAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAGCAGTA CCACATCCGG CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAA 
CAGCAACAAC AGCAACACCA GCAGCAACAG CAGCAACAGG CGGCTCCTCC CCAACCCCAG 
CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG GGGCTTCAGC AGACCCAGCA GCAGCAACAG 
ACAGCAGCTT TGGTCCGGCA ACTTCAACAA CAGCTCTCTA ATACCCAGCC ACAGCCCAGT 
ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009019.1
CDS	1..6084
Translation	MRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	NM_001009019.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009019.1

ORF Insert Sequence:

ATGCGGGCTG CCTGGCTCAT TAAGATGACC TGTGCCTACT ATGCAGCAAT CTCTGAGACC 
AAGGTTAAGA AGAGACATGT TGACCCTTTC ATGGAATGGA CTCAGATCAT CACCAAGTAC 
TTATGGGAGC AGTTACAGAA GATGGCTGAA TACTACCGGC CAGGGCCTGC AGGAAGTGGG 
GGCTGTGGTT CCACGATAGG GCCCTTGCCC CATGATGTAG AGGTGGCAAT CCGGCAGTGG 
GATTACACCG AGAAGCTGGC CATGTTCATG TTTCAGGATG GAATGCTGGA CAGACATGAG 
TTCCTGACCT GGGTGCTTGA GTGTTTTGAG AAGATCCGCC CTGGAGAGGA TGAATTGCTT 
AAACTGCTGC TGCCTCTGCT TCTCCGATAC TCTGGGGAAT TTGTTCAGTC TGCATACCTG 
TCCCGCCGGC TTGCCTACTT CTGTACACGG AGACTGGCCC TGCAGCTGGA TGGTGTGAGC 
AGTCACTCAT CTCATGTTAT ATCTGCTCAG TCAACAAGCA CGCTACCCAC CACCCCTGCT 
CCTCAGCCCC CAACTAGCAG CACACCCTCG ACTCCCTTTA GTGACCTGCT TATGTGCCCT 
CAGCACCGGC CCCTGGTTTT TGGCCTCAGC TGTATCCTAC AGACCATCCT CCTGTGCTGT 
CCTAGTGCCT TGGTTTGGCA CTACTCACTG ACTGATAGCA GAATTAAGAC CGGCTCACCA 
CTTGACCACT TGCCTATTGC CCCGTCCAAC CTGCCCATGC CAGAGGGTAA CAGTGCCTTC 
ACTCAGCAGG TCCGTGCAAA GTTGCGGGAG ATCGAGCAGC AGATCAAGGA GCGGGGACAG 
GCAGTTGAAG TTCGCTGGTC TTTCGATAAA TGCCAGGAAG CTACTGCAGG CTTCACCATT 
GGACGGGTAC TTCATACTTT GGAAGTGCTG GACAGCCATA GTTTTGAGCG CTCTGACTTC 
AGCAACTCTC TTGACTCCCT TTGTAACCGA ATCTTTGGAT TGGGGCCTAG CAAGGATGGG 
CATGAGATCT CCTCAGATGA TGATGCTGTG GTGTCATTGC TATGTGAATG GGCTGTCAGC 
TGCAAGCGTT CTGGTCGGCA TCGTGCTATG GTGGTAGCCA AGCTCCTGGA GAAGAGACAG 
GCGGAGATTG AGGCTGAGCG TTGTGGAGAA TCAGAAGCCG CAGATGAGAA GGGTTCCATC 
GCCTCTGGCT CCCTTTCTGC TCCCAGTGCT CCCATTTTCC AGGATGTCCT CCTGCAGTTT 
CTGGATACAC AGGCTCCCAT GCTGACGGAC CCTCGAAGTG AGAATGAGCG GGTGGAATTC 
TTTAACTTAG TACTGCTGTT CTGTGAACTG ATTCGACATG ATGTTTTCTC CCACAACATG 
TATACTTGCA CTCTCATCTC CCGAGGGGAC CTTGCCTTTG GAGCCCCTGG TCCCCGGCCT 
CCCTCTCCCT TTGATGATCC TGCCGATGAC GCAGAGCACA AGGAGGCTGA AGGCAGCAGC 
AGCAGCAAGC TGGAAGATCC AGGGCTCTCA GAATCTATGG ACATTGACCC TAGTTCCAGT 
GTGCTCTTTG AGGACATGGA GAAGCCTGAT TTCTCATTGT TCTCCCCTAC TATGCCCTGT 
GAGGGGAAGG GCAGTCCATC CCCTGAGAAG CCAGATGTCG AGAAGGAGGT GAAGCCCCCA 
CCCAAGGAGA AGATTGAAGG GACCCTTGGG ATTCTTTATG ACCAGCCACG ACACGTGCAG 
TACGCCACCC ATTTTCCCAT CCCCCAGGAG GAGTCATGCA GCCATGAGTG CAACCAGCGG 
TTGGTCGTGC TGTTTGGGGT GGGAAAGCAG CGAGATGATG CCCGCCATGC CATCAAGAAA 
ATCACCAAGG ATATCTTGAA GGTTCTGAAC CGCAAAGGGA CAGCAGAAAC TGACCAGCTT 
GCTCCTATTG TGCCTCTGAA TCCTGGAGAC CTGACATTCT TAGGTGGGGA GGATGGGCAG 
AAGCGGCGAC GCAACCGGCC TGAAGCCTTC CCCACTGCTG AAGATATCTT TGCTAAGTTC 
CAGCACCTTT CACATTATGA CCAACACCAG GTCACGGCTC AGGTCTCCCG GAATGTTCTG 
GAGCAGATCA CGAGCTTTGC CCTTGGCATG TCATACCACT TGCCTCTGGT GCAGCATGTG 
CAGTTCATCT TCGACCTCAT GGAATATTCA CTCAGCATCA GTGGCCTCAT CGACTTTGCC 
ATTCAGCTGC TGAATGAACT GAGTGTAGTT GAGGCTGAGC TGCTTCTCAA ATCCTCGGAT 
CTGGTGGGCA GCTACACTAC TAGCCTGTGC CTGTGCATCG TGGCTGTCCT GCGGCACTAT 
CATGCCTGCC TCATCCTCAA CCAGGACCAG ATGGCACAGG TCTTTGAGGG GCTGTGTGGC 
GTCGTGAAGC ATGGGATGAA CCGGTCCGAT GGCTCCTCTG CAGAGCGCTG TATCCTTGCT 
TATCTCTATG ATCTGTACAC CTCTTGTAGC CATTTAAAGA ACAAATTTGG GGAGCTCTTC 
AGCGACTTTT GCTCAAAGGT GAAGAACACC ATCTACTGCA ACGTGGAGCC ATCGGAATCA 
AATATGCGCT GGGCACCTGA GTTCATGATC GACACTCTAG AGAACCCTGC AGCTCACACC 
TTCACCTACA CGGGGCTAGG CAAGAGTCTT AGTGAGAACC CTGCTAACCG CTACAGCTTT 
GTCTGCAATG CCCTTATGCA CGTCTGTGTG GGGCACCATG ATCCCGATAG GGTGAATGAC 
ATCGCAATCC TGTGTGCAGA GCTGACCGGC TATTGCAAGT CACTGAGTGC AGAATGGCTA 
GGAGTGCTTA AGGCCTTGTG CTGCTCCTCT AACAATGGCA CTTGTGGTTT CAACGATCTC 
CTCTGCAATG TTGATGTCAG TGACCTGTCT TTTCATGACT CGCTGGCTAC TTTTGTTGCC 
ATCCTCATCG CTCGGCAGTG TTTGCTCCTG GAAGATCTGA TTCGCTGTGC TGCCATCCCT 
TCACTCCTTA ATGCTGCTTG TAGTGAACAG GACTCTGAGC CAGGGGCCCG GCTTACCTGC 
CGCATCCTCC TTCACCTTTT CAAGACACCG CAGCTCAATC CTTGCCAGTC TGATGGAAAC 
AAGCCTACAG TAGGAATCCG CTCCTCCTGC GACCGCCACC TGCTGGCTGC CTCCCAGAAC 
CGCATCGTGG ATGGAGCCGT GTTTGCTGTT CTGAAGGCTG TGTTTGTACT TGGGGATGCG 
GAACTGAAAG GTTCAGGCTT CACTGTGACA GGAGGAACAG AAGAACTTCC AGAGGAGGAG 
GGAGGAGGTG GCAGTGGTGG TCGGAGGCAG GGTGGCCGCA ACATCTCTGT GGAGACAGCC 
AGTCTGGACG TCTATGCCAA GTACGTGCTG CGCAGCATCT GCCAACAGGA ATGGGTAGGA 
GAACGTTGCC TTAAGTCTCT GTGTGAGGAC AGCAATGACC TGCAAGACCC AGTGTTGAGT 
AGTGCCCAGG CGCAGCGCCT CATGCAGCTC ATTTGCTATC CACATCGACT GCTGGACAAT 
GAGGATGGGG AAAACCCCCA GCGGCAGCGC ATAAAGCGCA TTCTCCAGAA CTTGGACCAG 
TGGACCATGC GCCAGTCTTC CTTGGAGCTG CAGCTCATGA TCAAGCAGAC CCCTAACAAT 
GAGATGAACT CCCTCTTGGA GAACATCGCC AAGGCCACAA TCGAGGTTTT CCAACAGTCA 
GCAGAGACAG GGTCATCTTC TGGAAGTACT GCAAGCAACA TGCCCAGCAG CAGCAAGACC 
AAGCCTGTGC TCAGCTCTCT AGAGCGCTCT GGTGTATGGC TGGTGGCCCC CCTTATTGCT 
AAACTGCCCA CCTCAGTCCA GGGACATGTG TTAAAGGCTG CTGGGGAAGA ATTGGAGAAG 
GGTCAGCACC TGGGTTCCTC TTCACGCAAA GAACGTGATC GACAAAAGCA GAAGAGCATG 
TCCCTATTGA GCCAGCAGCC CTTCTTATCG CTGGTGCTAA CATGTCTGAA AGGGCAGGAT 
GAACAACGCG AGGGACTCCT TACCTCCCTC TACAGCCAGG TGCACCAGAT TGTGAATAAT 
TGGCGAGATG ACCAGTACTT AGATGATTGC AAACCAAAGC AGCTTATGCA TGAGGCACTC 
AAACTGCGGC TCAACCTGGT GGGGGGCATG TTTGACACGG TGCAGCGCAG CACCCAGCAG 
ACCACGGAGT GGGCCATGCT CCTCCTGGAG ATCATCATCA GCGGCACTGT CGACATGCAG 
TCCAACAATG AGCTCTTCAC TACTGTGTTG GACATGCTGA GCGTGCTCAT CAATGGGACA 
TTGGCTGCAG ACATGTCTAG CATCTCACAA GGTAGCATGG AGGAAAACAA GCGTGCATAC 
ATGAACCTGG CGAAGAAGTT GCAGAAGGAG TTGGGGGAGC GCCAGTCGGA CAGTCTGGAA 
AAGGTTCGCC AGCTGCTGCC ACTGCCCAAG CAGACCCGAG ATGTCATCAC GTGTGAGCCA 
CAGGGCTCCC TTATCGATAC CAAGGGCAAC AAGATTGCTG GCTTCGATTC CATCTTCAAG 
AAGGAGGGTC TACAGGTTTC CACCAAACAG AAGATCTCGC CCTGGGATCT TTTCGAGGGG 
TTGAAGCCGT CAGCACCACT CTCTTGGGGC TGGTTTGGAA CAGTCCGAGT GGACCGGCGA 
GTGGCTCGAG GAGAGGAGCA GCAGCGGTTG CTGCTCTACC ACACACACCT GAGGCCCCGG 
CCCCGCGCCT ATTACCTGGA GCCACTGCCA CTGCCCCCAG AAGATGAGGA GCCGCCTGCT 
CCTACCCTGC TAGAGCCTGA GAAAAAGGCT CCAGAGCCCC CCAAAACTGA CAAACCGGGG 
GCTGCTCCAC CCAGTACTGA GGAACGCAAG AAGAAGTCCA CCAAGGGCAA GAAACGCAGC 
CAGCCAGCTA CCAAGACAGA GGACTATGGA ATGGGCCCGG GTCGGAGCGG CCCTTATGGT 
GTGACAGTGC CTCCGGACCT CCTGCACCAC CCAAACCCTG GTTCTATAAC ACACCTTAAC 
TATAGGCAAG GCTCCATAGG CCTGTACACC CAGAACCAGC CACTACCTGC AGGTGGCCCT 
CGTGTGGACC CATACCGCCC TGTGCGCTTA CCAATGCAGA AGCTGCCCAC CCGACCGACT 
TACCCTGGAG TGCTGCCCAC AACCATGACT GGCGTCATGG GTTTAGAACC CTCCTCTTAT 
AAGACCTCTG TGTACCGGCA GCAGCAACCT GCGGTGCCCC AAGGACAGCG CCTTCGCCAA 
CAGCTCCAGG CAAAGATACA GAGTCAGGGC ATGTTGGGAC AGTCATCTGT CCATCAGATG 
ACTCCCAGCT CTTCCTACGG TTTGCAGACT TCCCAGGGCT ATACTCCTTA TGTTTCTCAT 
GTGGGATTGC AGCAACACAC AGGCCCTGCA GGTACCATGG TGCCCCCCAG CTACTCCAGC 
CAGCCTTACC AGAGCACCCA CCCTTCTACC AATCCTACTC TTGTAGATCC TACCCGCCAC 
CTGCAACAGC GGCCCAGTGG CTATGTGCAC CAGCAGGCCC CCACCTATGG ACATGGACTG 
ACCTCCACTC AAAGGTTTTC ACACCAGACA CTGCAGCAGA CACCCATGAT AAGTACCATG 
ACTCCAATGA GTGCCCAGGG CGTCCAGGCA GGCGTCCGCT CAACAGCCAT CCTACCTGAG 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAACAGCAGC AGCAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAGCAGTA CCACATCCGG CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAA 
CAGCAACAAC AGCAACACCA GCAGCAACAG CAGCAACAGG CGGCTCCTCC CCAACCCCAG 
CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG GGGCTTCAGC AGACCCAGCA GCAGCAACAG 
ACAGCAGCTT TGGTCCGGCA ACTTCAACAA CAGCTCTCTA ATACCCAGCC ACAGCCCAGT 
ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942621.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798110.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

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CloneID	OPc90093
Clone ID Related Accession (Same CDS sequence)	XM_016942621.1 , XM_016942621.2
Accession Version	XM_016942621.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC 
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC 
GTCCAGGCAG GCGTCCGCTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG 
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC 
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA 
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798110.1
CDS	601..7143
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942621.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942621.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC 
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC 
GTCCAGGCAG GCGTCCGCTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG 
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC 
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA 
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942621.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798110.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

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CloneID	OPc90093
Clone ID Related Accession (Same CDS sequence)	XM_016942621.1 , XM_016942621.2
Accession Version	XM_016942621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC 
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC 
GTCCAGGCAG GCGTCCGCTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG 
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC 
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA 
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798110.1
CDS	429..6971
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942621.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942621.2

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC 
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC 
GTCCAGGCAG GCGTCCGCTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG 
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC 
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA 
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942622.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798111.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

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CloneID	OPc90094
Clone ID Related Accession (Same CDS sequence)	XM_016942622.1 , XM_016942622.2
Accession Version	XM_016942622.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGCT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798111.1
CDS	601..7134
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942622.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942622.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGCT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942622.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798111.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

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CloneID	OPc90094
Clone ID Related Accession (Same CDS sequence)	XM_016942622.1 , XM_016942622.2
Accession Version	XM_016942622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGCT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798111.1
CDS	429..6962
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942622.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942622.2

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGCT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942623.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798112.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

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CloneID	OPc90095
Clone ID Related Accession (Same CDS sequence)	XM_016942623.1 , XM_016942623.2
Accession Version	XM_016942623.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG 
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG 
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC 
CGCTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG 
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA 
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT 
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC 
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798112.1
CDS	601..7068
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942623.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942623.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG 
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG 
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC 
CGCTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG 
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA 
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT 
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC 
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_016942623.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798112.1 Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

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CloneID	OPc90095
Clone ID Related Accession (Same CDS sequence)	XM_016942623.1 , XM_016942623.2
Accession Version	XM_016942623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG 
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG 
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC 
CGCTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG 
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA 
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT 
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC 
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798112.1
CDS	64..6531
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSENERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDAEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_016942623.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942623.2

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAATGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACG CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGA TTCTTTATGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTGCT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCTTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTGTCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TGAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGACGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTTATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCGGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTCGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ATAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGCCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCGACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG 
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG 
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC 
CGCTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG 
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA 
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT 
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC 
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Major chimpanzee-specific structural changes in sperm development-associated genes.
Functional & integrative genomics11(3)507-17(2011 Sep)
Kim RN,Kim DW,Choi SH,Chae SH,Nam SH,Kim DW,Kim A,Kang A,Park KH,Lee YS,Hirai M,Suzuki Y,Sugano S,Hashimoto K,Kim DS,Park HS

Gene diversity patterns at 10 X-chromosomal loci in humans and chimpanzees.
Molecular biology and evolution20(8)1281-9(2003 Aug)
Kitano T,Schwarz C,Nickel B,P??bo S

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MED12 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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