MED12 cDNA ORF clone, Bos taurus(cattle)

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238390 XM_024988024.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa238391 XM_024988025.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa238392 XM_024988026.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa238393 XM_024988027.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa238394 XM_024988028.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa238395 XM_024988029.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa36933 NM_001205878.1
Latest version!
Bos taurus mediator complex subunit 12 (MED12), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa35484 XM_005228019.4
Latest version!
Bos taurus mediator complex subunit 12 (MED12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OBa35484 XM_005228019.3 Bos taurus mediator complex subunit 12 (MED12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238390
    Clone ID Related Accession (Same CDS sequence) XM_024988024.1
    Accession Version XM_024988024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGGCAAA GATAAGTCAA GGGATGTTGG GACAGTCATC TGTCCATCAG
    ATGACTCCCA GTTCTTCGTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT
    CATGTGGGAT TGCAGCAACA CACAGGCCCT GCAGGTACCA TGGTGCCCCC CAGCTACTCC
    AGCCAGCCTT ACCAGAGCAC CCACCCTTCT ACCAATCCTA CTCTTGTAGA TCCTACTCGC
    CACCTGCAGC AGCGGCCCAG TGGCTATGTG CACCAGCAGG CCCCAACCTA CGGACATGGG
    CTGACCTCCA CTCAAAGGTT TTCCCACCAG ACACTGCAGC AAACACCCAT GATAGGCACC
    ATGACCCCAC TGGGCCCCCA GGGTGTCCAG GCTGGCATCC GGTCGGCTTC CATCCTGCCT
    GAACAGCAGC AGCAGCAGCA GCAACAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA ACAGTACCAT ATCCGGCAGC AGCAGCAGCA GATCCTGCGG
    CAGCAGCAAC AGCAGCAGCA GCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAACAGCAG
    CAGCAACAAG CACACCAGCA GCAGCAGCAG CAGGCGGCTC CTCCTCAGCC CCAGCCCCAG
    TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT CAGCAGACAC AGCAACAACA ACAGACAGCA
    GCTTTGGTCC GGCAGCTCCA ACAACAGCTC TCTAACACCC AGCCACAGCC CAGTACCAAC
    ATATTTGGAC GCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843792.1
    CDS102..6719
    Translation

    Target ORF information:

    RefSeq Version XM_024988024.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGGCAAA GATAAGTCAA GGGATGTTGG GACAGTCATC TGTCCATCAG
    ATGACTCCCA GTTCTTCGTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT
    CATGTGGGAT TGCAGCAACA CACAGGCCCT GCAGGTACCA TGGTGCCCCC CAGCTACTCC
    AGCCAGCCTT ACCAGAGCAC CCACCCTTCT ACCAATCCTA CTCTTGTAGA TCCTACTCGC
    CACCTGCAGC AGCGGCCCAG TGGCTATGTG CACCAGCAGG CCCCAACCTA CGGACATGGG
    CTGACCTCCA CTCAAAGGTT TTCCCACCAG ACACTGCAGC AAACACCCAT GATAGGCACC
    ATGACCCCAC TGGGCCCCCA GGGTGTCCAG GCTGGCATCC GGTCGGCTTC CATCCTGCCT
    GAACAGCAGC AGCAGCAGCA GCAACAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA ACAGTACCAT ATCCGGCAGC AGCAGCAGCA GATCCTGCGG
    CAGCAGCAAC AGCAGCAGCA GCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAACAGCAG
    CAGCAACAAG CACACCAGCA GCAGCAGCAG CAGGCGGCTC CTCCTCAGCC CCAGCCCCAG
    TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT CAGCAGACAC AGCAACAACA ACAGACAGCA
    GCTTTGGTCC GGCAGCTCCA ACAACAGCTC TCTAACACCC AGCCACAGCC CAGTACCAAC
    ATATTTGGAC GCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238391
    Clone ID Related Accession (Same CDS sequence) XM_024988025.1
    Accession Version XM_024988025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGAGTCA AGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC
    AGTTCTTCGT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA
    TTGCAGCAAC ACACAGGCCC TGCAGGTACC ATGGTGCCCC CCAGCTACTC CAGCCAGCCT
    TACCAGAGCA CCCACCCTTC TACCAATCCT ACTCTTGTAG ATCCTACTCG CCACCTGCAG
    CAGCGGCCCA GTGGCTATGT GCACCAGCAG GCCCCAACCT ACGGACATGG GCTGACCTCC
    ACTCAAAGGT TTTCCCACCA GACACTGCAG CAAACACCCA TGATAGGCAC CATGACCCCA
    CTGGGCCCCC AGGGTGTCCA GGCTGGCATC CGGTCGGCTT CCATCCTGCC TGAACAGCAG
    CAGCAGCAGC AGCAACAGCA GCAACAGCAA CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AACAGTACCA TATCCGGCAG CAGCAGCAGC AGATCCTGCG GCAGCAGCAA
    CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA
    GCACACCAGC AGCAGCAGCA GCAGGCGGCT CCTCCTCAGC CCCAGCCCCA GTCCCAGCCC
    CAGTTCCAGC GCCAGGGGCT TCAGCAGACA CAGCAACAAC AACAGACAGC AGCTTTGGTC
    CGGCAGCTCC AACAACAGCT CTCTAACACC CAGCCACAGC CCAGTACCAA CATATTTGGA
    CGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843793.1
    CDS102..6710
    Translation

    Target ORF information:

    RefSeq Version XM_024988025.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGAGTCA AGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC
    AGTTCTTCGT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA
    TTGCAGCAAC ACACAGGCCC TGCAGGTACC ATGGTGCCCC CCAGCTACTC CAGCCAGCCT
    TACCAGAGCA CCCACCCTTC TACCAATCCT ACTCTTGTAG ATCCTACTCG CCACCTGCAG
    CAGCGGCCCA GTGGCTATGT GCACCAGCAG GCCCCAACCT ACGGACATGG GCTGACCTCC
    ACTCAAAGGT TTTCCCACCA GACACTGCAG CAAACACCCA TGATAGGCAC CATGACCCCA
    CTGGGCCCCC AGGGTGTCCA GGCTGGCATC CGGTCGGCTT CCATCCTGCC TGAACAGCAG
    CAGCAGCAGC AGCAACAGCA GCAACAGCAA CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AACAGTACCA TATCCGGCAG CAGCAGCAGC AGATCCTGCG GCAGCAGCAA
    CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA
    GCACACCAGC AGCAGCAGCA GCAGGCGGCT CCTCCTCAGC CCCAGCCCCA GTCCCAGCCC
    CAGTTCCAGC GCCAGGGGCT TCAGCAGACA CAGCAACAAC AACAGACAGC AGCTTTGGTC
    CGGCAGCTCC AACAACAGCT CTCTAACACC CAGCCACAGC CCAGTACCAA CATATTTGGA
    CGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238392
    Clone ID Related Accession (Same CDS sequence) XM_024988026.1
    Accession Version XM_024988026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGGCAAA GATAAGTCAA GGGATGTTGG GACAGTCATC TGTCCATCAG
    ATGACTCCCA GTTCTTCGTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT
    CATGTGGGAT TGCAGCAACA CACAGGCCCT GCAGATCCTA CTCGCCACCT GCAGCAGCGG
    CCCAGTGGCT ATGTGCACCA GCAGGCCCCA ACCTACGGAC ATGGGCTGAC CTCCACTCAA
    AGGTTTTCCC ACCAGACACT GCAGCAAACA CCCATGATAG GCACCATGAC CCCACTGGGC
    CCCCAGGGTG TCCAGGCTGG CATCCGGTCG GCTTCCATCC TGCCTGAACA GCAGCAGCAG
    CAGCAGCAAC AGCAGCAACA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAACAGT ACCATATCCG GCAGCAGCAG CAGCAGATCC TGCGGCAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAAGCACAC
    CAGCAGCAGC AGCAGCAGGC GGCTCCTCCT CAGCCCCAGC CCCAGTCCCA GCCCCAGTTC
    CAGCGCCAGG GGCTTCAGCA GACACAGCAA CAACAACAGA CAGCAGCTTT GGTCCGGCAG
    CTCCAACAAC AGCTCTCTAA CACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843794.1
    CDS102..6644
    Translation

    Target ORF information:

    RefSeq Version XM_024988026.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGATACT TTTCCCTCTC
    CTGCAAGCCT TCAAGGTCTG TGTTGTATTT TCCAAGTTTC AGCAGCTCAC TATTTCTCAT
    TTTCTGGAGC AGGGTCTTCA GGTTTCCACC AAACAAAAGA TCTCTCCCTG GGATCTTTTT
    GAGGGCTTGA AGCCATCAGC GCCACTGTCT TGGGGCTGGT TTGGAACAGT CCGGGTGGAC
    CGGCGCGTGG CCCGTGGAGA GGAGCAGCAG CGGCTGCTGC TGTACCACAC ACACCTGAGG
    CCCCGGCCCC GCGCCTACTA CCTGGAGCCA CTGCCACTGC CTCCAGAAGA CGAGGAACCC
    CCTGCCCCCA CCCTGCTAGA GCCTGAAAAA AAGGCTCCAG AGCCCCCCAA AACTGACAAA
    CCTGGAGCTG CTCCACCCAG CACTGAGGAA CGCAAGAAGA AGTCCACCAA GGGCAAGAAG
    CGCAGCCAGC CTGCCGCCAA GACGGAAGAC TATGGAATGG GCCCAGGCCG AAGTGGCCCC
    TACGGAGTGA CAGTGCCTCC AGACCTCCTG CACCATGCCA ACCCTAGCTC CATCTCCCAC
    CTTAGCTACA GGCAGAGCTC CATAGGCCTC TACACCCAGA ACCAGCCACT GCCAGCAGGT
    GGCCCCCGTG TGGATCCATA CCGCCCTGTG CGGTTACCGA TGCAGAAGCT GCCTACCCGC
    CCACCTTACC CTGGAGTGCT GCCCACGACC ATGACTGGTG TCATGGGACT GGAACCTGCC
    TCCTACAAGA CATCTGTGTA CCGACAGCAG CAGCCTGCAG TGCCCCAAGG ACAGCGCCTT
    CGCCAACAGC TCCAGGCAAA GATAAGTCAA GGGATGTTGG GACAGTCATC TGTCCATCAG
    ATGACTCCCA GTTCTTCGTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT
    CATGTGGGAT TGCAGCAACA CACAGGCCCT GCAGATCCTA CTCGCCACCT GCAGCAGCGG
    CCCAGTGGCT ATGTGCACCA GCAGGCCCCA ACCTACGGAC ATGGGCTGAC CTCCACTCAA
    AGGTTTTCCC ACCAGACACT GCAGCAAACA CCCATGATAG GCACCATGAC CCCACTGGGC
    CCCCAGGGTG TCCAGGCTGG CATCCGGTCG GCTTCCATCC TGCCTGAACA GCAGCAGCAG
    CAGCAGCAAC AGCAGCAACA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAACAGT ACCATATCCG GCAGCAGCAG CAGCAGATCC TGCGGCAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAAGCACAC
    CAGCAGCAGC AGCAGCAGGC GGCTCCTCCT CAGCCCCAGC CCCAGTCCCA GCCCCAGTTC
    CAGCGCCAGG GGCTTCAGCA GACACAGCAA CAACAACAGA CAGCAGCTTT GGTCCGGCAG
    CTCCAACAAC AGCTCTCTAA CACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238393
    Clone ID Related Accession (Same CDS sequence) XM_024988027.1
    Accession Version XM_024988027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGAG TCAAGGGATG
    TTGGGACAGT CATCTGTCCA TCAGATGACT CCCAGTTCTT CGTACGGTTT GCAGACCTCC
    CAGGGCTATA CTCCTTATGT TTCTCATGTG GGATTGCAGC AACACACAGG CCCTGCAGGT
    ACCATGGTGC CCCCCAGCTA CTCCAGCCAG CCTTACCAGA GCACCCACCC TTCTACCAAT
    CCTACTCTTG TAGATCCTAC TCGCCACCTG CAGCAGCGGC CCAGTGGCTA TGTGCACCAG
    CAGGCCCCAA CCTACGGACA TGGGCTGACC TCCACTCAAA GGTTTTCCCA CCAGACACTG
    CAGCAAACAC CCATGATAGG CACCATGACC CCACTGGGCC CCCAGGGTGT CCAGGCTGGC
    ATCCGGTCGG CTTCCATCCT GCCTGAACAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACAGTA CCATATCCGG
    CAGCAGCAGC AGCAGATCCT GCGGCAGCAG CAACAGCAGC AGCAGCAGCA ACAGCAGCAA
    CAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAAGCACACC AGCAGCAGCA GCAGCAGGCG
    GCTCCTCCTC AGCCCCAGCC CCAGTCCCAG CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG
    ACACAGCAAC AACAACAGAC AGCAGCTTTG GTCCGGCAGC TCCAACAACA GCTCTCTAAC
    ACCCAGCCAC AGCCCAGTAC CAACATATTT GGACGCTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843795.1
    CDS214..6735
    Translation

    Target ORF information:

    RefSeq Version XM_024988027.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGAG TCAAGGGATG
    TTGGGACAGT CATCTGTCCA TCAGATGACT CCCAGTTCTT CGTACGGTTT GCAGACCTCC
    CAGGGCTATA CTCCTTATGT TTCTCATGTG GGATTGCAGC AACACACAGG CCCTGCAGGT
    ACCATGGTGC CCCCCAGCTA CTCCAGCCAG CCTTACCAGA GCACCCACCC TTCTACCAAT
    CCTACTCTTG TAGATCCTAC TCGCCACCTG CAGCAGCGGC CCAGTGGCTA TGTGCACCAG
    CAGGCCCCAA CCTACGGACA TGGGCTGACC TCCACTCAAA GGTTTTCCCA CCAGACACTG
    CAGCAAACAC CCATGATAGG CACCATGACC CCACTGGGCC CCCAGGGTGT CCAGGCTGGC
    ATCCGGTCGG CTTCCATCCT GCCTGAACAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACAGTA CCATATCCGG
    CAGCAGCAGC AGCAGATCCT GCGGCAGCAG CAACAGCAGC AGCAGCAGCA ACAGCAGCAA
    CAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAAGCACACC AGCAGCAGCA GCAGCAGGCG
    GCTCCTCCTC AGCCCCAGCC CCAGTCCCAG CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG
    ACACAGCAAC AACAACAGAC AGCAGCTTTG GTCCGGCAGC TCCAACAACA GCTCTCTAAC
    ACCCAGCCAC AGCCCAGTAC CAACATATTT GGACGCTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238394
    Clone ID Related Accession (Same CDS sequence) XM_024988028.1
    Accession Version XM_024988028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGAG TCAAGGGATG
    TTGGGACAGT CATCTGTCCA TCAGATGACT CCCAGTTCTT CGTACGGTTT GCAGACCTCC
    CAGGGCTATA CTCCTTATGT TTCTCATGTG GGATTGCAGC AACACACAGG CCCTGCAGAT
    CCTACTCGCC ACCTGCAGCA GCGGCCCAGT GGCTATGTGC ACCAGCAGGC CCCAACCTAC
    GGACATGGGC TGACCTCCAC TCAAAGGTTT TCCCACCAGA CACTGCAGCA AACACCCATG
    ATAGGCACCA TGACCCCACT GGGCCCCCAG GGTGTCCAGG CTGGCATCCG GTCGGCTTCC
    ATCCTGCCTG AACAGCAGCA GCAGCAGCAG CAACAGCAGC AACAGCAACA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGTACCATA TCCGGCAGCA GCAGCAGCAG
    ATCCTGCGGC AGCAGCAACA GCAGCAGCAG CAGCAACAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAACAAGC ACACCAGCAG CAGCAGCAGC AGGCGGCTCC TCCTCAGCCC
    CAGCCCCAGT CCCAGCCCCA GTTCCAGCGC CAGGGGCTTC AGCAGACACA GCAACAACAA
    CAGACAGCAG CTTTGGTCCG GCAGCTCCAA CAACAGCTCT CTAACACCCA GCCACAGCCC
    AGTACCAACA TATTTGGACG CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843796.1
    CDS203..6649
    Translation

    Target ORF information:

    RefSeq Version XM_024988028.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGAG TCAAGGGATG
    TTGGGACAGT CATCTGTCCA TCAGATGACT CCCAGTTCTT CGTACGGTTT GCAGACCTCC
    CAGGGCTATA CTCCTTATGT TTCTCATGTG GGATTGCAGC AACACACAGG CCCTGCAGAT
    CCTACTCGCC ACCTGCAGCA GCGGCCCAGT GGCTATGTGC ACCAGCAGGC CCCAACCTAC
    GGACATGGGC TGACCTCCAC TCAAAGGTTT TCCCACCAGA CACTGCAGCA AACACCCATG
    ATAGGCACCA TGACCCCACT GGGCCCCCAG GGTGTCCAGG CTGGCATCCG GTCGGCTTCC
    ATCCTGCCTG AACAGCAGCA GCAGCAGCAG CAACAGCAGC AACAGCAACA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGTACCATA TCCGGCAGCA GCAGCAGCAG
    ATCCTGCGGC AGCAGCAACA GCAGCAGCAG CAGCAACAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAACAAGC ACACCAGCAG CAGCAGCAGC AGGCGGCTCC TCCTCAGCCC
    CAGCCCCAGT CCCAGCCCCA GTTCCAGCGC CAGGGGCTTC AGCAGACACA GCAACAACAA
    CAGACAGCAG CTTTGGTCCG GCAGCTCCAA CAACAGCTCT CTAACACCCA GCCACAGCCC
    AGTACCAACA TATTTGGACG CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238395
    Clone ID Related Accession (Same CDS sequence) XM_024988029.1
    Accession Version XM_024988029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGGCCG CCTGGCTCAT TAAGATGACC TGTGCCTACT ATGCAGCAAT CTCAGAGACC 
    AAGGTTAAGA AGAGACATAT TGACCCCTTC ACAGAATGGA CTCAGATCAT CACCAAGTGC
    TTATGGGAGC AGCTTCAAAA GATGGCTGAA TACTACCGGC CAGGGCCTGC TGGAAGTGGG
    GGCTGTGGCT CCACTATAGG CCCCTTGCCC CATGATGTTG AGATGGCAAT CCGGCAGTGG
    GACTACAACG AGAAGCTGGC CATGTTCATG TTTCAGGACG GAATGCTGGA CAGACATGAG
    TTTCTGACCT GGGTACTTGA GTGTTTTGAG AAAATCCGCC CTGGAGAGGA TGAATTGCTT
    AAACTGCTGC TGCCCCTGCT GCTTCGATAC TCTGGAGAGT TTGTTCAGTC TGCGTACCTC
    TCCCGCCGCC TCGCCTACTT CTGTACACGG AGACTGGCCC TGCAGCTGGA TGGTGTGAGC
    AGTCACTCAT CTCATGTGAT GTCTGCTCAG TCGACAAGCA CACTGCCCAC CACCCCTGCT
    CCTCAGCCCC CAAGTAGCAG CACACCCTCT ACACCCTTTA GTGACCTGCT TATGTGCCCT
    CAGCACCGGC CCCTAGTTTT TGGCCTCAGC TGTATCCTTC AGACCATCCT GCTGTGTTGT
    CCTAGTGCCC TGGTTTGGCA CTATTCACTG ACTGATAGTC GAATCAAGAC TGGCTCACCA
    CTTGACCACC TGCCTATTGC CCCCTCCAAC CTGCCCATGC CAGAGGGCAA CAGTGCCTTC
    ACTCAGCAGG TCCGTGCAAA GTTGCGGGAG ATCGAGCAAC AGATCAAGGA GCGAGGACAG
    GCCGTTGAGG TTCGCTGGTC TTTTGATAAG TGCCAGGAAG CTACTGCAGG CTTCACCATT
    GGACGAGTAC TCCATACTTT GGAGGTGTTG GACAGCCATA GTTTTGAACG CTCTGACTTC
    AGCAACTCTC TTGATTCCCT CTGTAATCGA ATCTTTGGAT TGGGGCCTAG CAAGGATGGG
    CACGAGATCT CCTCAGATGA TGATGCAGTG GTATCGTTAC TGTGTGAATG GGCTGTCAGC
    TGCAAGCGTT CTGGTCGGCA TCGTGCGATG GTGGTAGCCA AGCTGCTGGA GAAGAGACAG
    GCAGAGATTG AGGCTGAGCG TTGTGGAGAA TCGGAAGCTG CAGATGAGAA GGGTTCCATT
    GCCTCTGGCT CCCTTTCTGC TCCCAGTGCT CCCATTTTCC AAGATGTCCT CTTACAGTTT
    CTGGATACAC AGGCTCCCAT GCTGACGGAC CCCCGAAGTG AGAGTGAGCG AGTGGAATTC
    TTTAACTTGG TACTGCTGTT CTGTGAACTG ATTCGACATG ATGTTTTCTC CCACAACATG
    TACACTTGCA CCCTCATCTC CCGAGGGGAC CTTGCCTTCG GAGCCCCTGG TCCCCGGCCT
    CCCTCTCCCT TTGATGACCC AGCTGATGAC CCAGAGCGCA AGGAGGCTGA GGGCAGCAGC
    AGCAGCAAGC TGGAGGATCC AGGGCTCTCA GAGTCTATGG ACATTGACCC TAGCTCCAGT
    GTGCTCTTTG AGGACATGGA AAAGCCTGAT TTCTCATTGT TCTCCCCTAC TATGCCCTGT
    GAGGGGAAGG GCAGTCCATC CCCTGAGAAA CCAGATGTTG AGAAGGAGGT GAAGCCCCCA
    CCCAAGGAGA AGCTAGAAGG AACCCTTGGG GTTCTTTATG ACCAGCCACG GCATGTGCAG
    TATGCCACAC ACTTTCCCAT CCCCCAGGAG GAGTCATGCA GCCATGAGTG CAACCAGCGG
    TTGGTCGTAC TGTTTGGGGT GGGAAAGCAG CGAGATGATG CCCGCCATGC CATCAAGAAA
    ATTACCAAGG ATATCCTGAA GGTTCTGAAC CGCAAGGGGA CAGCAGAAAC TGACCAGCTT
    GCTCCTATTG TGCCTCTGAA TCCTGGAGAC CTGACATTCT TAGGTGGGGA GGATGGACAG
    AAGCGGCGAC GAAACCGACC TGAAGCTTTT CCCACTGCCG AGGATATCTT TGCTAAGTTC
    CAGCACCTTT CACATTATGA CCAACATCAG GTCACGGCTC AGGTCTCCCG GAATGTTCTG
    GAGCAGATCA CGAGCTTTGC CCTTGGCATG TCATACCACT TGCCTCTGGT GCAGCATGTG
    CAGTTTATCT TCGACCTCAT GGAATATTCA CTCAGCATCA GTGGCCTCAT CGACTTTGCC
    ATTCAGCTGC TGAATGAACT GAGTGTAGTC GAGGCCGAAC TGCTTCTCAA ATCCTCAGAT
    CTGGTGGGCA GCTACACCAC CAGCCTGTGC CTGTGCATCG TGGCTGTCCT GCGCCACTAT
    CATGCCTGCC TCATCCTCAA CCAGGACCAG ATGGCACAGG TCTTTGAGGG GCTGTGTGGC
    GTAGTTAAGC ATGGGATGAA CCGATCTGAT GGTTCCTCTG CAGAACGCTG TATCCTTGCA
    TATCTCTATG ATCTGTACAC CTCCTGTAGC CATTTAAAGA GCAAATTTGG GGAACTCTTC
    AGTGACTTCT GCTCCAAGGT GAAGAACACC ATCTACTGCA ACGTGGAGCC GTCAGAGTCC
    AACATGCGCT GGGCACCCGA GTTCATGATC GACACTTTAG AGAACCCCGC CGCTCACACC
    TTCACCTACA CGGGGCTAGG CAAGAGTCTT AGTGAGAACC CTGCTAACCG CTACAGCTTT
    GTCTGCAATG CCCTTATGCA CGTCTGTGTG GGGCACCATG ATTCGGATAG GGTGAATGAC
    ATCGCCATCC TGTGTGCAGA GCTGACCGGC TATTGCAAGT CACTGAGTGC AGAGTGGCTG
    GGAGTGCTTA AGGCCTTGTG CTGCTCCTCT AACAATGGCA CTTGTGGTTT CAACGACCTC
    CTCTGCAATG TAGATGTCAG TGACCTGTCT TTTCACGACT CCCTGGCCAC TTTTGTTGCC
    ATCCTCATCG CTCGGCAGTG TTTGCTCCTG GAGGATCTGA TTCGCTGTGC GGCCATCCCT
    TCACTCCTTA ATGCTGCTTG CAGTGAACAG GACTCTGAGC CAGGGGCCCG GCTTACCTGC
    CGCATCCTCC TCCACCTTTT CAAGACACCT CAACTCAATC CTTGCCAATC GGATGGAAAC
    AAGCCTACTG TAGGAATCCG CTCCTCCTGT GACCGCCACC TGCTGGCTGC CTCCCAGAAC
    CGCATCGTGG ATGGAGCTGT GTTTGCTGTT CTCAAGGCTG TGTTTGTACT TGGGGATGCG
    GAACTGAAGG GTTCGGGCTT CACTGTGACA GGAGGAACAG AAGAGCTTCC AGAGGAGGAA
    GGAGGAGGTG GCAGTGGCAG TCGGAGGCAG GGTGGCCGCA ACATCTCTGT GGAGACAGCC
    AGTCTGGATG TCTATGCCAA GTACGTGCTA CGCAGCATCT GCCAGCAGGA ATGGGTAGGA
    GAACGTTGCC TTAAATCGCT GTGTGAGGAC AGCAATGACC TGCAAGACCC AGTGTTGAGC
    AGTGCCCAGG CCCAGCGCCT CATGCAGCTT ATCTGCTACC CACATCGGCT GCTGGACAAC
    GAGGATGGGG AAAACCCACA GCGGCAACGC ATTAAGCGTA TTCTCCAGAA CTTGGACCAG
    TGGACCATGC GCCAGTCTTC ATTGGAACTG CAGCTCATGA TCAAGCAGAC CCCTAACAAT
    GAGATGAACT CCCTCTTAGA GAACATCGCC AAGGCCACAA TCGAGGTTTT CCAACAGTCT
    GCAGAGACAG GGTCATCTTC TGGAAATGCT GCAAGCAACA TGCCCAGCAG CAGCAAGACC
    AAGCCTGTGC TCAGCTCTCT AGAACGCTCG GGTGTATGGC TAGTGGCTCC TCTCATTGCC
    AAACTGCCCA CCTCAGTCCA GGGGCATGTG TTAAAGGCTG CTGGGGAAGA ATTGGAGAAC
    GGCCAGCACC TGGACTCCTC TTCCCGCAAA GAACGTGATC GACAAAAGCA AAAGAGCATG
    TCCCTGTTGA GCCAGCAGCC CTTCTTATCC CTGGTGCTGA CATGTCTGAA GGGGCAGGAT
    GAGCAGCGTG AGGGACTCCT TACCTCGCTC TACAGCCAGG TCCACCAGAT TGTGACTAAT
    TGGAGAGATG ACCAGTATTT AGACGATTGC AAACCAAAGC AGCTAATGCA TGAGGCACTC
    AAACTGCGGC TCAACCTGGT GGGGGGCATG TTTGACACAG TGCAGCGCAG CACCCAGCAG
    ACCACGGAGT GGGCTGTGCT CCTCCTGGAG ATCATCATCA GCGGCACTGT CGACATGCAG
    TCCAACAACG AGCTCTTCAC CACTGTCCTG GACATGCTAA GCGTGCTAAT CAATGGGACC
    CTAGCTGCAG ACATGTCCAG CATCTCCCAG GGCAGCATGG AGGAAAACAA ACGTGCCTAC
    ATGAACCTGG TGAAGAAGCT GCGGAAAGAG TTGGCGGAAC GCCAGTCGGA TAGTCTGGAG
    AAAGTTTACC AGCTGCTGCC ACTGCCCAAG CCGACTCGAG ATGTGATCAC GTGTGAGCCG
    CAGGGCTCAC TTATTGACAC CAAGGGCAAC AAGATTGCTG GCTTCGACTC CATCTTCAAG
    AAGGAGGGTC TTCAGGTTTC CACCAAACAA AAGATCTCTC CCTGGGATCT TTTTGAGGGC
    TTGAAGCCAT CAGCGCCACT GTCTTGGGGC TGGTTTGGAA CAGTCCGGGT GGACCGGCGC
    GTGGCCCGTG GAGAGGAGCA GCAGCGGCTG CTGCTGTACC ACACACACCT GAGGCCCCGG
    CCCCGCGCCT ACTACCTGGA GCCACTGCCA CTGCCTCCAG AAGACGAGGA ACCCCCTGCC
    CCCACCCTGC TAGAGCCTGA AAAAAAGGCT CCAGAGCCCC CCAAAACTGA CAAACCTGGA
    GCTGCTCCAC CCAGCACTGA GGAACGCAAG AAGAAGTCCA CCAAGGGCAA GAAGCGCAGC
    CAGCCTGCCG CCAAGACGGA AGACTATGGA ATGGGCCCAG GCCGAAGTGG CCCCTACGGA
    GTGACAGTGC CTCCAGACCT CCTGCACCAT GCCAACCCTA GCTCCATCTC CCACCTTAGC
    TACAGGCAGA GCTCCATAGG CCTCTACACC CAGAACCAGC CACTGCCAGC AGGTGGCCCC
    CGTGTGGATC CATACCGCCC TGTGCGGTTA CCGATGCAGA AGCTGCCTAC CCGCCCACCT
    TACCCTGGAG TGCTGCCCAC GACCATGACT GGTGTCATGG GACTGGAACC TGCCTCCTAC
    AAGACATCTG TGTACCGACA GCAGCAGCCT GCAGTGCCCC AAGGACAGCG CCTTCGCCAA
    CAGCTCCAGG CAAAGATAAG TCAAGGGATG TTGGGACAGT CATCTGTCCA TCAGATGACT
    CCCAGTTCTT CGTACGGTTT GCAGACCTCC CAGGGCTATA CTCCTTATGT TTCTCATGTG
    GGATTGCAGC AACACACAGG CCCTGCAGGT ACCATGGTGC CCCCCAGCTA CTCCAGCCAG
    CCTTACCAGA GCACCCACCC TTCTACCAAT CCTACTCTTG TAGATCCTAC TCGCCACCTG
    CAGCAGCGGC CCAGTGGCTA TGTGCACCAG CAGGCCCCAA CCTACGGACA TGGGCTGACC
    TCCACTCAAA GGTTTTCCCA CCAGACACTG CAGCAAACAC CCATGATAGG CACCATGACC
    CCACTGGGCC CCCAGGGTGT CCAGGCTGGC ATCCGGTCGG CTTCCATCCT GCCTGAACAG
    CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAACAGTA CCATATCCGG CAGCAGCAGC AGCAGATCCT GCGGCAGCAG
    CAACAGCAGC AGCAGCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAACA GCAGCAGCAA
    CAAGCACACC AGCAGCAGCA GCAGCAGGCG GCTCCTCCTC AGCCCCAGCC CCAGTCCCAG
    CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG ACACAGCAAC AACAACAGAC AGCAGCTTTG
    GTCCGGCAGC TCCAACAACA GCTCTCTAAC ACCCAGCCAC AGCCCAGTAC CAACATATTT
    GGACGCTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843797.1
    CDS576..6647
    Translation

    Target ORF information:

    RefSeq Version XM_024988029.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGGCCG CCTGGCTCAT TAAGATGACC TGTGCCTACT ATGCAGCAAT CTCAGAGACC 
    AAGGTTAAGA AGAGACATAT TGACCCCTTC ACAGAATGGA CTCAGATCAT CACCAAGTGC
    TTATGGGAGC AGCTTCAAAA GATGGCTGAA TACTACCGGC CAGGGCCTGC TGGAAGTGGG
    GGCTGTGGCT CCACTATAGG CCCCTTGCCC CATGATGTTG AGATGGCAAT CCGGCAGTGG
    GACTACAACG AGAAGCTGGC CATGTTCATG TTTCAGGACG GAATGCTGGA CAGACATGAG
    TTTCTGACCT GGGTACTTGA GTGTTTTGAG AAAATCCGCC CTGGAGAGGA TGAATTGCTT
    AAACTGCTGC TGCCCCTGCT GCTTCGATAC TCTGGAGAGT TTGTTCAGTC TGCGTACCTC
    TCCCGCCGCC TCGCCTACTT CTGTACACGG AGACTGGCCC TGCAGCTGGA TGGTGTGAGC
    AGTCACTCAT CTCATGTGAT GTCTGCTCAG TCGACAAGCA CACTGCCCAC CACCCCTGCT
    CCTCAGCCCC CAAGTAGCAG CACACCCTCT ACACCCTTTA GTGACCTGCT TATGTGCCCT
    CAGCACCGGC CCCTAGTTTT TGGCCTCAGC TGTATCCTTC AGACCATCCT GCTGTGTTGT
    CCTAGTGCCC TGGTTTGGCA CTATTCACTG ACTGATAGTC GAATCAAGAC TGGCTCACCA
    CTTGACCACC TGCCTATTGC CCCCTCCAAC CTGCCCATGC CAGAGGGCAA CAGTGCCTTC
    ACTCAGCAGG TCCGTGCAAA GTTGCGGGAG ATCGAGCAAC AGATCAAGGA GCGAGGACAG
    GCCGTTGAGG TTCGCTGGTC TTTTGATAAG TGCCAGGAAG CTACTGCAGG CTTCACCATT
    GGACGAGTAC TCCATACTTT GGAGGTGTTG GACAGCCATA GTTTTGAACG CTCTGACTTC
    AGCAACTCTC TTGATTCCCT CTGTAATCGA ATCTTTGGAT TGGGGCCTAG CAAGGATGGG
    CACGAGATCT CCTCAGATGA TGATGCAGTG GTATCGTTAC TGTGTGAATG GGCTGTCAGC
    TGCAAGCGTT CTGGTCGGCA TCGTGCGATG GTGGTAGCCA AGCTGCTGGA GAAGAGACAG
    GCAGAGATTG AGGCTGAGCG TTGTGGAGAA TCGGAAGCTG CAGATGAGAA GGGTTCCATT
    GCCTCTGGCT CCCTTTCTGC TCCCAGTGCT CCCATTTTCC AAGATGTCCT CTTACAGTTT
    CTGGATACAC AGGCTCCCAT GCTGACGGAC CCCCGAAGTG AGAGTGAGCG AGTGGAATTC
    TTTAACTTGG TACTGCTGTT CTGTGAACTG ATTCGACATG ATGTTTTCTC CCACAACATG
    TACACTTGCA CCCTCATCTC CCGAGGGGAC CTTGCCTTCG GAGCCCCTGG TCCCCGGCCT
    CCCTCTCCCT TTGATGACCC AGCTGATGAC CCAGAGCGCA AGGAGGCTGA GGGCAGCAGC
    AGCAGCAAGC TGGAGGATCC AGGGCTCTCA GAGTCTATGG ACATTGACCC TAGCTCCAGT
    GTGCTCTTTG AGGACATGGA AAAGCCTGAT TTCTCATTGT TCTCCCCTAC TATGCCCTGT
    GAGGGGAAGG GCAGTCCATC CCCTGAGAAA CCAGATGTTG AGAAGGAGGT GAAGCCCCCA
    CCCAAGGAGA AGCTAGAAGG AACCCTTGGG GTTCTTTATG ACCAGCCACG GCATGTGCAG
    TATGCCACAC ACTTTCCCAT CCCCCAGGAG GAGTCATGCA GCCATGAGTG CAACCAGCGG
    TTGGTCGTAC TGTTTGGGGT GGGAAAGCAG CGAGATGATG CCCGCCATGC CATCAAGAAA
    ATTACCAAGG ATATCCTGAA GGTTCTGAAC CGCAAGGGGA CAGCAGAAAC TGACCAGCTT
    GCTCCTATTG TGCCTCTGAA TCCTGGAGAC CTGACATTCT TAGGTGGGGA GGATGGACAG
    AAGCGGCGAC GAAACCGACC TGAAGCTTTT CCCACTGCCG AGGATATCTT TGCTAAGTTC
    CAGCACCTTT CACATTATGA CCAACATCAG GTCACGGCTC AGGTCTCCCG GAATGTTCTG
    GAGCAGATCA CGAGCTTTGC CCTTGGCATG TCATACCACT TGCCTCTGGT GCAGCATGTG
    CAGTTTATCT TCGACCTCAT GGAATATTCA CTCAGCATCA GTGGCCTCAT CGACTTTGCC
    ATTCAGCTGC TGAATGAACT GAGTGTAGTC GAGGCCGAAC TGCTTCTCAA ATCCTCAGAT
    CTGGTGGGCA GCTACACCAC CAGCCTGTGC CTGTGCATCG TGGCTGTCCT GCGCCACTAT
    CATGCCTGCC TCATCCTCAA CCAGGACCAG ATGGCACAGG TCTTTGAGGG GCTGTGTGGC
    GTAGTTAAGC ATGGGATGAA CCGATCTGAT GGTTCCTCTG CAGAACGCTG TATCCTTGCA
    TATCTCTATG ATCTGTACAC CTCCTGTAGC CATTTAAAGA GCAAATTTGG GGAACTCTTC
    AGTGACTTCT GCTCCAAGGT GAAGAACACC ATCTACTGCA ACGTGGAGCC GTCAGAGTCC
    AACATGCGCT GGGCACCCGA GTTCATGATC GACACTTTAG AGAACCCCGC CGCTCACACC
    TTCACCTACA CGGGGCTAGG CAAGAGTCTT AGTGAGAACC CTGCTAACCG CTACAGCTTT
    GTCTGCAATG CCCTTATGCA CGTCTGTGTG GGGCACCATG ATTCGGATAG GGTGAATGAC
    ATCGCCATCC TGTGTGCAGA GCTGACCGGC TATTGCAAGT CACTGAGTGC AGAGTGGCTG
    GGAGTGCTTA AGGCCTTGTG CTGCTCCTCT AACAATGGCA CTTGTGGTTT CAACGACCTC
    CTCTGCAATG TAGATGTCAG TGACCTGTCT TTTCACGACT CCCTGGCCAC TTTTGTTGCC
    ATCCTCATCG CTCGGCAGTG TTTGCTCCTG GAGGATCTGA TTCGCTGTGC GGCCATCCCT
    TCACTCCTTA ATGCTGCTTG CAGTGAACAG GACTCTGAGC CAGGGGCCCG GCTTACCTGC
    CGCATCCTCC TCCACCTTTT CAAGACACCT CAACTCAATC CTTGCCAATC GGATGGAAAC
    AAGCCTACTG TAGGAATCCG CTCCTCCTGT GACCGCCACC TGCTGGCTGC CTCCCAGAAC
    CGCATCGTGG ATGGAGCTGT GTTTGCTGTT CTCAAGGCTG TGTTTGTACT TGGGGATGCG
    GAACTGAAGG GTTCGGGCTT CACTGTGACA GGAGGAACAG AAGAGCTTCC AGAGGAGGAA
    GGAGGAGGTG GCAGTGGCAG TCGGAGGCAG GGTGGCCGCA ACATCTCTGT GGAGACAGCC
    AGTCTGGATG TCTATGCCAA GTACGTGCTA CGCAGCATCT GCCAGCAGGA ATGGGTAGGA
    GAACGTTGCC TTAAATCGCT GTGTGAGGAC AGCAATGACC TGCAAGACCC AGTGTTGAGC
    AGTGCCCAGG CCCAGCGCCT CATGCAGCTT ATCTGCTACC CACATCGGCT GCTGGACAAC
    GAGGATGGGG AAAACCCACA GCGGCAACGC ATTAAGCGTA TTCTCCAGAA CTTGGACCAG
    TGGACCATGC GCCAGTCTTC ATTGGAACTG CAGCTCATGA TCAAGCAGAC CCCTAACAAT
    GAGATGAACT CCCTCTTAGA GAACATCGCC AAGGCCACAA TCGAGGTTTT CCAACAGTCT
    GCAGAGACAG GGTCATCTTC TGGAAATGCT GCAAGCAACA TGCCCAGCAG CAGCAAGACC
    AAGCCTGTGC TCAGCTCTCT AGAACGCTCG GGTGTATGGC TAGTGGCTCC TCTCATTGCC
    AAACTGCCCA CCTCAGTCCA GGGGCATGTG TTAAAGGCTG CTGGGGAAGA ATTGGAGAAC
    GGCCAGCACC TGGACTCCTC TTCCCGCAAA GAACGTGATC GACAAAAGCA AAAGAGCATG
    TCCCTGTTGA GCCAGCAGCC CTTCTTATCC CTGGTGCTGA CATGTCTGAA GGGGCAGGAT
    GAGCAGCGTG AGGGACTCCT TACCTCGCTC TACAGCCAGG TCCACCAGAT TGTGACTAAT
    TGGAGAGATG ACCAGTATTT AGACGATTGC AAACCAAAGC AGCTAATGCA TGAGGCACTC
    AAACTGCGGC TCAACCTGGT GGGGGGCATG TTTGACACAG TGCAGCGCAG CACCCAGCAG
    ACCACGGAGT GGGCTGTGCT CCTCCTGGAG ATCATCATCA GCGGCACTGT CGACATGCAG
    TCCAACAACG AGCTCTTCAC CACTGTCCTG GACATGCTAA GCGTGCTAAT CAATGGGACC
    CTAGCTGCAG ACATGTCCAG CATCTCCCAG GGCAGCATGG AGGAAAACAA ACGTGCCTAC
    ATGAACCTGG TGAAGAAGCT GCGGAAAGAG TTGGCGGAAC GCCAGTCGGA TAGTCTGGAG
    AAAGTTTACC AGCTGCTGCC ACTGCCCAAG CCGACTCGAG ATGTGATCAC GTGTGAGCCG
    CAGGGCTCAC TTATTGACAC CAAGGGCAAC AAGATTGCTG GCTTCGACTC CATCTTCAAG
    AAGGAGGGTC TTCAGGTTTC CACCAAACAA AAGATCTCTC CCTGGGATCT TTTTGAGGGC
    TTGAAGCCAT CAGCGCCACT GTCTTGGGGC TGGTTTGGAA CAGTCCGGGT GGACCGGCGC
    GTGGCCCGTG GAGAGGAGCA GCAGCGGCTG CTGCTGTACC ACACACACCT GAGGCCCCGG
    CCCCGCGCCT ACTACCTGGA GCCACTGCCA CTGCCTCCAG AAGACGAGGA ACCCCCTGCC
    CCCACCCTGC TAGAGCCTGA AAAAAAGGCT CCAGAGCCCC CCAAAACTGA CAAACCTGGA
    GCTGCTCCAC CCAGCACTGA GGAACGCAAG AAGAAGTCCA CCAAGGGCAA GAAGCGCAGC
    CAGCCTGCCG CCAAGACGGA AGACTATGGA ATGGGCCCAG GCCGAAGTGG CCCCTACGGA
    GTGACAGTGC CTCCAGACCT CCTGCACCAT GCCAACCCTA GCTCCATCTC CCACCTTAGC
    TACAGGCAGA GCTCCATAGG CCTCTACACC CAGAACCAGC CACTGCCAGC AGGTGGCCCC
    CGTGTGGATC CATACCGCCC TGTGCGGTTA CCGATGCAGA AGCTGCCTAC CCGCCCACCT
    TACCCTGGAG TGCTGCCCAC GACCATGACT GGTGTCATGG GACTGGAACC TGCCTCCTAC
    AAGACATCTG TGTACCGACA GCAGCAGCCT GCAGTGCCCC AAGGACAGCG CCTTCGCCAA
    CAGCTCCAGG CAAAGATAAG TCAAGGGATG TTGGGACAGT CATCTGTCCA TCAGATGACT
    CCCAGTTCTT CGTACGGTTT GCAGACCTCC CAGGGCTATA CTCCTTATGT TTCTCATGTG
    GGATTGCAGC AACACACAGG CCCTGCAGGT ACCATGGTGC CCCCCAGCTA CTCCAGCCAG
    CCTTACCAGA GCACCCACCC TTCTACCAAT CCTACTCTTG TAGATCCTAC TCGCCACCTG
    CAGCAGCGGC CCAGTGGCTA TGTGCACCAG CAGGCCCCAA CCTACGGACA TGGGCTGACC
    TCCACTCAAA GGTTTTCCCA CCAGACACTG CAGCAAACAC CCATGATAGG CACCATGACC
    CCACTGGGCC CCCAGGGTGT CCAGGCTGGC ATCCGGTCGG CTTCCATCCT GCCTGAACAG
    CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAACAGTA CCATATCCGG CAGCAGCAGC AGCAGATCCT GCGGCAGCAG
    CAACAGCAGC AGCAGCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAACA GCAGCAGCAA
    CAAGCACACC AGCAGCAGCA GCAGCAGGCG GCTCCTCCTC AGCCCCAGCC CCAGTCCCAG
    CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG ACACAGCAAC AACAACAGAC AGCAGCTTTG
    GTCCGGCAGC TCCAACAACA GCTCTCTAAC ACCCAGCCAC AGCCCAGTAC CAACATATTT
    GGACGCTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36933
    Clone ID Related Accession (Same CDS sequence) NM_001205878.1
    Accession Version NM_001205878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000030.1. On Apr 20, 2011 this sequence version replaced XM_599229.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.46141.1, SRR6764281.8306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGGTA CCATGGTGCC CCCCAGCTAC TCCAGCCAGC CTTACCAGAG CACCCACCCT
    TCTACCAATC CTACTCTTGT AGATCCTACT CGCCACCTGC AGCAGCGGCC CAGTGGCTAT
    GTGCACCAGC AGGCCCCAAC CTACGGACAT GGGCTGACCT CCACTCAAAG GTTTTCCCAC
    CAGACACTGC AGCAAACACC CATGATAGGC ACCATGACCC CACTGGGCCC CCAGGGTGTC
    CAGGCTGGCA TCCGGTCGGC TTCCATCCTG CCTGAACAGC AGCAGCAGCA GCAGCAACAG
    CAGCAACAGC AACAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGTAC
    CATATCCGGC AGCAGCAGCA GCAGATCCTG CGGCAGCAGC AACAGCAGCA GCAGCAGCAA
    CAGCAGCAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AAGCACACCA GCAGCAGCAG
    CAGCAGGCGG CTCCTCCTCA GCCCCAGCCC CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG
    CTTCAGCAGA CACAGCAACA ACAACAGACA GCAGCTTTGG TCCGGCAGCT CCAACAACAG
    CTCTCTAACA CCCAGCCACA GCCCAGTACC AACATATTTG GACGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192807.1
    CDS1..6531
    Translation

    Target ORF information:

    RefSeq Version NM_001205878.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGGTA CCATGGTGCC CCCCAGCTAC TCCAGCCAGC CTTACCAGAG CACCCACCCT
    TCTACCAATC CTACTCTTGT AGATCCTACT CGCCACCTGC AGCAGCGGCC CAGTGGCTAT
    GTGCACCAGC AGGCCCCAAC CTACGGACAT GGGCTGACCT CCACTCAAAG GTTTTCCCAC
    CAGACACTGC AGCAAACACC CATGATAGGC ACCATGACCC CACTGGGCCC CCAGGGTGTC
    CAGGCTGGCA TCCGGTCGGC TTCCATCCTG CCTGAACAGC AGCAGCAGCA GCAGCAACAG
    CAGCAACAGC AACAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGTAC
    CATATCCGGC AGCAGCAGCA GCAGATCCTG CGGCAGCAGC AACAGCAGCA GCAGCAGCAA
    CAGCAGCAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AAGCACACCA GCAGCAGCAG
    CAGCAGGCGG CTCCTCCTCA GCCCCAGCCC CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG
    CTTCAGCAGA CACAGCAACA ACAACAGACA GCAGCTTTGG TCCGGCAGCT CCAACAACAG
    CTCTCTAACA CCCAGCCACA GCCCAGTACC AACATATTTG GACGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35484
    Clone ID Related Accession (Same CDS sequence) XM_005228019.4 , XM_005228019.3
    Accession Version XM_005228019.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGATC CTACTCGCCA CCTGCAGCAG CGGCCCAGTG GCTATGTGCA CCAGCAGGCC
    CCAACCTACG GACATGGGCT GACCTCCACT CAAAGGTTTT CCCACCAGAC ACTGCAGCAA
    ACACCCATGA TAGGCACCAT GACCCCACTG GGCCCCCAGG GTGTCCAGGC TGGCATCCGG
    TCGGCTTCCA TCCTGCCTGA ACAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGTACCATAT CCGGCAGCAG
    CAGCAGCAGA TCCTGCGGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA GCAACAAGCA CACCAGCAGC AGCAGCAGCA GGCGGCTCCT
    CCTCAGCCCC AGCCCCAGTC CCAGCCCCAG TTCCAGCGCC AGGGGCTTCA GCAGACACAG
    CAACAACAAC AGACAGCAGC TTTGGTCCGG CAGCTCCAAC AACAGCTCTC TAACACCCAG
    CCACAGCCCA GTACCAACAT ATTTGGACGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228076.1
    CDS38..6493
    Translation

    Target ORF information:

    RefSeq Version XM_005228019.4
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228019.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGATC CTACTCGCCA CCTGCAGCAG CGGCCCAGTG GCTATGTGCA CCAGCAGGCC
    CCAACCTACG GACATGGGCT GACCTCCACT CAAAGGTTTT CCCACCAGAC ACTGCAGCAA
    ACACCCATGA TAGGCACCAT GACCCCACTG GGCCCCCAGG GTGTCCAGGC TGGCATCCGG
    TCGGCTTCCA TCCTGCCTGA ACAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGTACCATAT CCGGCAGCAG
    CAGCAGCAGA TCCTGCGGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA GCAACAAGCA CACCAGCAGC AGCAGCAGCA GGCGGCTCCT
    CCTCAGCCCC AGCCCCAGTC CCAGCCCCAG TTCCAGCGCC AGGGGCTTCA GCAGACACAG
    CAACAACAAC AGACAGCAGC TTTGGTCCGG CAGCTCCAAC AACAGCTCTC TAACACCCAG
    CCACAGCCCA GTACCAACAT ATTTGGACGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35484
    Clone ID Related Accession (Same CDS sequence) XM_005228019.4 , XM_005228019.3
    Accession Version XM_005228019.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGATC CTACTCGCCA CCTGCAGCAG CGGCCCAGTG GCTATGTGCA CCAGCAGGCC
    CCAACCTACG GACATGGGCT GACCTCCACT CAAAGGTTTT CCCACCAGAC ACTGCAGCAA
    ACACCCATGA TAGGCACCAT GACCCCACTG GGCCCCCAGG GTGTCCAGGC TGGCATCCGG
    TCGGCTTCCA TCCTGCCTGA ACAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGTACCATAT CCGGCAGCAG
    CAGCAGCAGA TCCTGCGGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA GCAACAAGCA CACCAGCAGC AGCAGCAGCA GGCGGCTCCT
    CCTCAGCCCC AGCCCCAGTC CCAGCCCCAG TTCCAGCGCC AGGGGCTTCA GCAGACACAG
    CAACAACAAC AGACAGCAGC TTTGGTCCGG CAGCTCCAAC AACAGCTCTC TAACACCCAG
    CCACAGCCCA GTACCAACAT ATTTGGACGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228076.1
    CDS256..6711
    Translation

    Target ORF information:

    RefSeq Version XM_005228019.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228019.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
    CCTCCTGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAATTAAC GGCCTTGAAT
    GTAAAACAAG GTTTCAATAA CCAGCCCGCT GTCTCTGGGG ATGAACATGG CACTGCCAAG
    AATGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA ACAGCATCAT TACAGAGAAG
    TTACGTTGTA ACACCCTCCC TGACATTGGG CGAAGGAAGC CCCAAGTGAA CCAGAAGGAC
    AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT TACTGATCTG
    GCTGGTACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
    GAAGTGTTTG GGTATTTAGC CAAATACACA GTGCCTGTGA TGCGGGCCGC CTGGCTCATT
    AAGATGACCT GTGCCTACTA TGCAGCAATC TCAGAGACCA AGGTTAAGAA GAGACATATT
    GACCCCTTCA CAGAATGGAC TCAGATCATC ACCAAGTGCT TATGGGAGCA GCTTCAAAAG
    ATGGCTGAAT ACTACCGGCC AGGGCCTGCT GGAAGTGGGG GCTGTGGCTC CACTATAGGC
    CCCTTGCCCC ATGATGTTGA GATGGCAATC CGGCAGTGGG ACTACAACGA GAAGCTGGCC
    ATGTTCATGT TTCAGGACGG AATGCTGGAC AGACATGAGT TTCTGACCTG GGTACTTGAG
    TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCCCTGCTG
    CTTCGATACT CTGGAGAGTT TGTTCAGTCT GCGTACCTCT CCCGCCGCCT CGCCTACTTC
    TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTGATG
    TCTGCTCAGT CGACAAGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC AAGTAGCAGC
    ACACCCTCTA CACCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT
    GGCCTCAGCT GTATCCTTCA GACCATCCTG CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC
    TATTCACTGA CTGATAGTCG AATCAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC
    CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
    TTGCGGGAGA TCGAGCAACA GATCAAGGAG CGAGGACAGG CCGTTGAGGT TCGCTGGTCT
    TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGAGTACT CCATACTTTG
    GAGGTGTTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGATTCCCTC
    TGTAATCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC CTCAGATGAT
    GATGCAGTGG TATCGTTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
    CGTGCGATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT
    TGTGGAGAAT CGGAAGCTGC AGATGAGAAG GGTTCCATTG CCTCTGGCTC CCTTTCTGCT
    CCCAGTGCTC CCATTTTCCA AGATGTCCTC TTACAGTTTC TGGATACACA GGCTCCCATG
    CTGACGGACC CCCGAAGTGA GAGTGAGCGA GTGGAATTCT TTAACTTGGT ACTGCTGTTC
    TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ACACTTGCAC CCTCATCTCC
    CGAGGGGACC TTGCCTTCGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCA
    GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GCAGCAAGCT GGAGGATCCA
    GGGCTCTCAG AGTCTATGGA CATTGACCCT AGCTCCAGTG TGCTCTTTGA GGACATGGAA
    AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
    CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GCTAGAAGGA
    ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC
    CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
    GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TTACCAAGGA TATCCTGAAG
    GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
    CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGACAGA AGCGGCGACG AAACCGACCT
    GAAGCTTTTC CCACTGCCGA GGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
    CAACATCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
    CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTTATCTT CGACCTCATG
    GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
    AGTGTAGTCG AGGCCGAACT GCTTCTCAAA TCCTCAGATC TGGTGGGCAG CTACACCACC
    AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGCCACTATC ATGCCTGCCT CATCCTCAAC
    CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTTAAGCA TGGGATGAAC
    CGATCTGATG GTTCCTCTGC AGAACGCTGT ATCCTTGCAT ATCTCTATGA TCTGTACACC
    TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAACTCTTCA GTGACTTCTG CTCCAAGGTG
    AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAGTCCA ACATGCGCTG GGCACCCGAG
    TTCATGATCG ACACTTTAGA GAACCCCGCC GCTCACACCT TCACCTACAC GGGGCTAGGC
    AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
    GTCTGTGTGG GGCACCATGA TTCGGATAGG GTGAATGACA TCGCCATCCT GTGTGCAGAG
    CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAGTGGCTGG GAGTGCTTAA GGCCTTGTGC
    TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT
    GACCTGTCTT TTCACGACTC CCTGGCCACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
    TTGCTCCTGG AGGATCTGAT TCGCTGTGCG GCCATCCCTT CACTCCTTAA TGCTGCTTGC
    AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT CCACCTTTTC
    AAGACACCTC AACTCAATCC TTGCCAATCG GATGGAAACA AGCCTACTGT AGGAATCCGC
    TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG
    TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCGGGCTTC
    ACTGTGACAG GAGGAACAGA AGAGCTTCCA GAGGAGGAAG GAGGAGGTGG CAGTGGCAGT
    CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
    TACGTGCTAC GCAGCATCTG CCAGCAGGAA TGGGTAGGAG AACGTTGCCT TAAATCGCTG
    TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGCA GTGCCCAGGC CCAGCGCCTC
    ATGCAGCTTA TCTGCTACCC ACATCGGCTG CTGGACAACG AGGATGGGGA AAACCCACAG
    CGGCAACGCA TTAAGCGTAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCA
    TTGGAACTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTAGAG
    AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCTG CAGAGACAGG GTCATCTTCT
    GGAAATGCTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
    GAACGCTCGG GTGTATGGCT AGTGGCTCCT CTCATTGCCA AACTGCCCAC CTCAGTCCAG
    GGGCATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAACG GCCAGCACCT GGACTCCTCT
    TCCCGCAAAG AACGTGATCG ACAAAAGCAA AAGAGCATGT CCCTGTTGAG CCAGCAGCCC
    TTCTTATCCC TGGTGCTGAC ATGTCTGAAG GGGCAGGATG AGCAGCGTGA GGGACTCCTT
    ACCTCGCTCT ACAGCCAGGT CCACCAGATT GTGACTAATT GGAGAGATGA CCAGTATTTA
    GACGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
    GGGGGCATGT TTGACACAGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCTGTGCTC
    CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAACGA GCTCTTCACC
    ACTGTCCTGG ACATGCTAAG CGTGCTAATC AATGGGACCC TAGCTGCAGA CATGTCCAGC
    ATCTCCCAGG GCAGCATGGA GGAAAACAAA CGTGCCTACA TGAACCTGGT GAAGAAGCTG
    CGGAAAGAGT TGGCGGAACG CCAGTCGGAT AGTCTGGAGA AAGTTTACCA GCTGCTGCCA
    CTGCCCAAGC CGACTCGAGA TGTGATCACG TGTGAGCCGC AGGGCTCACT TATTGACACC
    AAGGGCAACA AGATTGCTGG CTTCGACTCC ATCTTCAAGA AGGAGGGTCT TCAGGTTTCC
    ACCAAACAAA AGATCTCTCC CTGGGATCTT TTTGAGGGCT TGAAGCCATC AGCGCCACTG
    TCTTGGGGCT GGTTTGGAAC AGTCCGGGTG GACCGGCGCG TGGCCCGTGG AGAGGAGCAG
    CAGCGGCTGC TGCTGTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA CTACCTGGAG
    CCACTGCCAC TGCCTCCAGA AGACGAGGAA CCCCCTGCCC CCACCCTGCT AGAGCCTGAA
    AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCTGGAG CTGCTCCACC CAGCACTGAG
    GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAGCGCAGCC AGCCTGCCGC CAAGACGGAA
    GACTATGGAA TGGGCCCAGG CCGAAGTGGC CCCTACGGAG TGACAGTGCC TCCAGACCTC
    CTGCACCATG CCAACCCTAG CTCCATCTCC CACCTTAGCT ACAGGCAGAG CTCCATAGGC
    CTCTACACCC AGAACCAGCC ACTGCCAGCA GGTGGCCCCC GTGTGGATCC ATACCGCCCT
    GTGCGGTTAC CGATGCAGAA GCTGCCTACC CGCCCACCTT ACCCTGGAGT GCTGCCCACG
    ACCATGACTG GTGTCATGGG ACTGGAACCT GCCTCCTACA AGACATCTGT GTACCGACAG
    CAGCAGCCTG CAGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATAAGT
    CAAGGGATGT TGGGACAGTC ATCTGTCCAT CAGATGACTC CCAGTTCTTC GTACGGTTTG
    CAGACCTCCC AGGGCTATAC TCCTTATGTT TCTCATGTGG GATTGCAGCA ACACACAGGC
    CCTGCAGATC CTACTCGCCA CCTGCAGCAG CGGCCCAGTG GCTATGTGCA CCAGCAGGCC
    CCAACCTACG GACATGGGCT GACCTCCACT CAAAGGTTTT CCCACCAGAC ACTGCAGCAA
    ACACCCATGA TAGGCACCAT GACCCCACTG GGCCCCCAGG GTGTCCAGGC TGGCATCCGG
    TCGGCTTCCA TCCTGCCTGA ACAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGTACCATAT CCGGCAGCAG
    CAGCAGCAGA TCCTGCGGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAGCAGCAGC AACAGCAGCA GCAACAAGCA CACCAGCAGC AGCAGCAGCA GGCGGCTCCT
    CCTCAGCCCC AGCCCCAGTC CCAGCCCCAG TTCCAGCGCC AGGGGCTTCA GCAGACACAG
    CAACAACAAC AGACAGCAGC TTTGGTCCGG CAGCTCCAAC AACAGCTCTC TAACACCCAG
    CCACAGCCCA GTACCAACAT ATTTGGACGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.