MED12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12
Entrez Gene ID	9968
Full Name	mediator complex subunit 12
Synonyms	ARC240,CAGH45,FGS1,HOPA,MED12S,OHDOX,OKS,OPA1,TNRC11,TRAP230
General protein information	Preferred Names mediator complex subunit 12 Names mediator of RNA polymerase II transcription subunit 12 CAG repeat protein 45 OPA-containing protein activator-recruited cofactor 240 kDa component human opposite paired mediator of RNA polymerase II transcription, subunit 12 homolog putative mediator subunit 12 thyroid hormone receptor-associated protein complex 230 kDa component thyroid hormone receptor-associated protein, 230 kDa subunit trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit) trinucleotide repeat-containing gene 11 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq13.1
Summary	The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009].
Disorder MIM:	300188
Disorder Html:	Opitz-Kaveggia syndrome, 305450 (3); Lujan-Fryns syndrome, 309520 (3); Ohdo syndrome, X-linked, 300895 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005120.3	NP_005111.2	mediator of RNA polymerase II transcription subunit 12
NM_005120.2	NP_005111.2	mediator of RNA polymerase II transcription subunit 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12	NP_005111.2
	MED12	XP_001088424.2
Rattus norvegicus (Norway rat)	Med12	NP_001180221.1
Danio rerio (zebrafish)	med12	NP_001034550.1
Pan troglodytes (chimpanzee)	MED12	NP_001009019.1
Bos taurus (cattle)	MED12	NP_001192807.1
Xenopus tropicalis (tropical clawed frog)	med12	XP_002934949.2
Gallus gallus (chicken)	MED12	XP_004940691.1
Mus musculus (house mouse)	Med12	NP_067496.2
Canis lupus familiaris (dog)	MED12	XP_003640267.1

Gene Ontology

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19668	NM_005120.3 Latest version!	Homo sapiens mediator complex subunit 12 (MED12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu19668	NM_005120.2	Homo sapiens mediator complex subunit 12 (MED12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12 ( NM_005120.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005111.2 Homo sapiens mediator complex subunit 12 (MED12), mRNA.

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CloneID	OHu19668
Clone ID Related Accession (Same CDS sequence)	NM_005120.3 , NM_005120.2
Accession Version	NM_005120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB069668.1, AF117755.1, AF071309.1 and BM662470.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005120.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374080.8/ ENSP00000363193.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005111.2
CDS	160..6693
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	NM_005120.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005120.3

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( NM_005120.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005111.2 Homo sapiens mediator complex subunit 12 (MED12), mRNA.

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CloneID	OHu19668
Clone ID Related Accession (Same CDS sequence)	NM_005120.3 , NM_005120.2
Accession Version	NM_005120.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB069668.1, AF117755.1, AF071309.1 and BM662470.1. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2006 this sequence version replaced NM_005120.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005111.2
CDS	200..6733
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	NM_005120.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005120.2

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG 
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA 
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT 
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG 
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT 
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG 
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG 
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT 
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC 
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA 
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC 
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC 
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC 
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT 
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT 
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT 
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA 
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG 
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG 
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT 
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC 
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG 
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT 
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT 
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC 
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG 
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC 
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG 
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT 
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG 
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT 
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC 
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT 
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC 
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT 
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC 
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG 
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA 
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC 
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC 
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG 
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG 
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG 
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG 
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC 
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC 
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT 
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA 
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG 
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC 
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT 
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA 
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC 
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC 
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA 
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA 
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG 
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG 
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG 
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA 
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MED12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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