col1a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa59790 XM_012964923.3
Latest version!
Xenopus tropicalis collagen type I alpha 2 (col1a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa22159 NM_001079250.1
Latest version!
Xenopus tropicalis collagen type I alpha 2 (col1a2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa29554 XM_012964923.2
Latest version!
Xenopus tropicalis collagen, type 1, alpha 2 (col1a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa59790
    Clone ID Related Accession (Same CDS sequence) XM_012964923.3
    Accession Version XM_012964923.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-2(I) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAATGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTC CTCCTGGAGC TTCTGGTCCT
    TCTGGTGTCC TTGGTGCTCT TGGTATCTCT GGTCTTCCAG GAGCAAGAGG TGAACGTGGT
    ACCCCTGGTG GCCCTGGAGC TAATGGTGAG TCAGGTCCTG CTGGCCCTGC AGGTTCTGCT
    GGTTCCCGTG GCCCCTCTGG CCCCGTGGGT TCTCCCGGTC TGACTGGTGT TCATGGTGAA
    GCTGGTCGTG ATGGCAATCC TGGTAATGAT GGTCCTCCCG GTCGTGATGG TCTTCCTGGA
    GTTAAGGGAG AACGTGGCTA TCCTGGAGGC ACTGGCCCTG CTGGATCTCT TGGTGCTGTT
    GGAGCTCCAG GAGCTGTTGG TCCTTCTGGA AAATCTGGAA ATCGTGGTGA ACCTGGTCCC
    GTTGGTCATG CTGGAGCTGT TGGCCCTGCT GGTCCCAGAG GCCCATCTGG ACAACAAGGT
    ACTCGTGGTG ACAAGGGTGA AGCTGGTGAG AGGGGAGCTA GAGGTTTGGA TGGACGCAAA
    GGACATAATG GTTTACAAGG TCTCCCTGGC CCTGCTGGTT CTCCAGGAGA AACAGGTCCT
    GCTGGTTCCA ACGGTCCATC TGGCCCACGG GGTCCAGCTG GCCCATCCGG TCCTCCAGGC
    AAAGAAGGAC GTTCCGGACA TAATGGTCCA ATTGGTCCAG TCGGTCTTCG TGGTCCAGCT
    GGTCACCAAG GTCCTGCTGG TCCTCCTGGA CCACCTGGTC TTCCTGGTCC TGCTGGCGCA
    TCTGGTGGTG GATATGATGG TGGCTTTGGT GGTGAATTCT TCAGAGCTGA CCAGCCTGCT
    CGTAGACCAA AGGATTATGA AGTTGATGCC ACTCTGAAAT CTTTGAACAA CCAAATTGAG
    ACAATCCTGA CCCCAGAAGG CTCAAAGAAG AACCCTGCCC GCACTTGCCG TGACTTAAGA
    CTCAGCCACC CAGATTGGAG CAGTGGATTC TACTGGATCG ATCCTAACCA AGGCTGCACA
    TCTGATTCCA TCAGAGTTTT CTGTGATTTC TCCACTGGTG AGACCTGCAT CCATGCCAAC
    CCAGACAGCA TTCCACAGAA GAACTGGTAC ACTAGCAAGA CCAAACAAAG GAAGCATGTA
    TGGTTCGGTG AAACCATCAA TGGTGGCACA CAGATTGAAT ACAACGAAGA AGGAGTCACA
    TCCAAGGACA TGGCCACACA GTTTGCTTTC ATGCGTTTAC TGGCTAATCA CGCATCTCAG
    AATATTACCT ACCACTGCAA GAACAGCATT GCCTATATGG ATGGACAGAC TGGAAACCTG
    AAGAAGGCCA TTTTGCTACA AGGATCCAAT GACGTTGAGC TACGCGCAGA GGGCAACAGC
    AGATTCACTT TCAGTGTCCT AGAAGATGGT TGCACGCAAC ATACGGGTGA ATGGGGAAGA
    ACAGTCATAG AATACAGAAC TAACAAACCA TCTCGCTTAC CCATCCTGGA CATTGCACCT
    TTGGACATTG GTGGTGATGA CCAAGAGTTT GGTCTGGACA TTGGCCCAGT CTGTTTCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820377.1
    CDS143..4045
    Translation

    Target ORF information:

    RefSeq Version XM_012964923.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type I alpha 2 (col1a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAATGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTC CTCCTGGAGC TTCTGGTCCT
    TCTGGTGTCC TTGGTGCTCT TGGTATCTCT GGTCTTCCAG GAGCAAGAGG TGAACGTGGT
    ACCCCTGGTG GCCCTGGAGC TAATGGTGAG TCAGGTCCTG CTGGCCCTGC AGGTTCTGCT
    GGTTCCCGTG GCCCCTCTGG CCCCGTGGGT TCTCCCGGTC TGACTGGTGT TCATGGTGAA
    GCTGGTCGTG ATGGCAATCC TGGTAATGAT GGTCCTCCCG GTCGTGATGG TCTTCCTGGA
    GTTAAGGGAG AACGTGGCTA TCCTGGAGGC ACTGGCCCTG CTGGATCTCT TGGTGCTGTT
    GGAGCTCCAG GAGCTGTTGG TCCTTCTGGA AAATCTGGAA ATCGTGGTGA ACCTGGTCCC
    GTTGGTCATG CTGGAGCTGT TGGCCCTGCT GGTCCCAGAG GCCCATCTGG ACAACAAGGT
    ACTCGTGGTG ACAAGGGTGA AGCTGGTGAG AGGGGAGCTA GAGGTTTGGA TGGACGCAAA
    GGACATAATG GTTTACAAGG TCTCCCTGGC CCTGCTGGTT CTCCAGGAGA AACAGGTCCT
    GCTGGTTCCA ACGGTCCATC TGGCCCACGG GGTCCAGCTG GCCCATCCGG TCCTCCAGGC
    AAAGAAGGAC GTTCCGGACA TAATGGTCCA ATTGGTCCAG TCGGTCTTCG TGGTCCAGCT
    GGTCACCAAG GTCCTGCTGG TCCTCCTGGA CCACCTGGTC TTCCTGGTCC TGCTGGCGCA
    TCTGGTGGTG GATATGATGG TGGCTTTGGT GGTGAATTCT TCAGAGCTGA CCAGCCTGCT
    CGTAGACCAA AGGATTATGA AGTTGATGCC ACTCTGAAAT CTTTGAACAA CCAAATTGAG
    ACAATCCTGA CCCCAGAAGG CTCAAAGAAG AACCCTGCCC GCACTTGCCG TGACTTAAGA
    CTCAGCCACC CAGATTGGAG CAGTGGATTC TACTGGATCG ATCCTAACCA AGGCTGCACA
    TCTGATTCCA TCAGAGTTTT CTGTGATTTC TCCACTGGTG AGACCTGCAT CCATGCCAAC
    CCAGACAGCA TTCCACAGAA GAACTGGTAC ACTAGCAAGA CCAAACAAAG GAAGCATGTA
    TGGTTCGGTG AAACCATCAA TGGTGGCACA CAGATTGAAT ACAACGAAGA AGGAGTCACA
    TCCAAGGACA TGGCCACACA GTTTGCTTTC ATGCGTTTAC TGGCTAATCA CGCATCTCAG
    AATATTACCT ACCACTGCAA GAACAGCATT GCCTATATGG ATGGACAGAC TGGAAACCTG
    AAGAAGGCCA TTTTGCTACA AGGATCCAAT GACGTTGAGC TACGCGCAGA GGGCAACAGC
    AGATTCACTT TCAGTGTCCT AGAAGATGGT TGCACGCAAC ATACGGGTGA ATGGGGAAGA
    ACAGTCATAG AATACAGAAC TAACAAACCA TCTCGCTTAC CCATCCTGGA CATTGCACCT
    TTGGACATTG GTGGTGATGA CCAAGAGTTT GGTCTGGACA TTGGCCCAGT CTGTTTCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22159
    Clone ID Related Accession (Same CDS sequence) NM_001079250.1
    Accession Version NM_001079250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-2(I) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125681.1. ##Evidence-Data-START## Transcript exon combination :: BC125681.1, BC168447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028293, SAMD00028294 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAATGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTA TTGTTGGTCC TTCCGGTGCT
    ACAGGCCATC CTGGTAAAGA TGGTCCAAGA GGTCCTCGTG GTGACAGTGG ACCAGTTGGC
    CGTCCTGGAG AACAAGGTAT CGGTGGTCCA CAAGGTTTGA TTGGAGAGAA GGGTTCATCT
    GGTGAATCTG GTCCTGCTGG TCCTCCTGGA GCTTCTGGTC CTTCTGGTGT CCTTGGTGCT
    CTTGGTATCT CTGGTCTTCC AGGAGCAAGA GGTGAACGTG GTACCCCTGG TGGCCCTGGA
    GCTAATGGTG AGTCAGGTCC TGCTGGCCCT GCAGGTTCTG CTGGTTCCCG TGGCCCCTCT
    GGCCCCGTGG GTTCTCCCGG TCTGACTGGT GTTCATGGTG AAGCTGGTCG TGATGGCAAT
    CCTGGTAATG ATGGTCCTCC CGGTCGTGAT GGTCTTCCTG GAGTTAAGGG AGAACGTGGC
    TATCCTGGAG GCACTGGCCC TGCTGGATCT CTTGGTGCTG TTGGAGCTCC AGGAGCTGTT
    GGTCCTTCTG GAAAATCTGG AAATCGTGGT GAACCTGGTC CCGTTGGTCA TGCTGGAGCT
    GTTGGCCCTG CTGGTCCCAG AGGCCCATCT GGACAACAAG GTACTCGTGG TGACAAGGGT
    GAAGCTGGTG AGAGGGGAGC TAGAGGTTTG GATGGACGCA AAGGACATAA TGGTTTACAA
    GGTCTCCCTG GCCCTGCTGG TTCTCCAGGA GAAACAGGTC CTGCTGGTTC CAACGGTCCA
    TCTGGCCCAC GGGGTCCAGC TGGCCCATCC GGTCCTCCAG GCAAAGAAGG ACGTTCCGGA
    CATAATGGTC CAATTGGTCC AGTCGGTCTT CGTGGTCCAG CTGGTCACCA AGGTCCTGCT
    GGTCCTCCTG GACCACCTGG TCTTCCTGGT CCTGCTGGCG CATCTGGTGG TGGATATGAT
    GGTGGCTTTG GTGGTGAATT CTTCAGAGCT GACCAGCCTG CTCGTAGACC AAAGGATTAT
    GAAGTTGATG CCACTCTGAA ATCTTTGAAC AACCAAATTG AGACAATCCT GACCCCAGAA
    GGCTCAAAGA AGAACCCTGC CCGCACTTGC CGTGACTTAA GACTCAGCCA CCCAGATTGG
    AGCAGTGGAT TCTACTGGAT CGATCCTAAC CAAGGCTGCA CATCTGATTC CATCAGAGTT
    TTCTGTGATT TCTCCACTGG TGAGACCTGC ATCCATGCCA ACCCAGACAG CATTCCACAG
    AAGAACTGGT ACACTAGCAA GACCAAACAA AGGAAGCATG TATGGTTCGG TGAAACCATC
    AATGGTGGCA CACAGATTGA ATACAACGAA GAAGGAGTCA CATCCAAGGA CATGGCCACA
    CAGTTTGCTT TCATGCGTTT ACTGGCTAAT CACGCATCTC AGAATATTAC CTACCACTGC
    AAGAACAGCA TTGCCTATAT GGATGGACAG ACTGGAAACC TGAAGAAGGC CATTTTGCTA
    CAAGGATCCA ATGACGTTGA GCTACGCGCA GAGGGCAACA GCAGATTCAC TTTCAGTGTC
    CTAGAAGATG GTTGCACGCA ACATACGGGT GAATGGGGAA GAACAGTCAT AGAATACAGA
    ACTAACAAAC CATCTCGCTT ACCCATCCTG GACATTGCAC CTTTGGACAT TGGTGGTGAT
    GACCAAGAGT TTGGTCTGGA CATTGGCCCA GTCTGTTTCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072718.1
    CDS117..4181
    Translation

    Target ORF information:

    RefSeq Version NM_001079250.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type I alpha 2 (col1a2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAATGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTA TTGTTGGTCC TTCCGGTGCT
    ACAGGCCATC CTGGTAAAGA TGGTCCAAGA GGTCCTCGTG GTGACAGTGG ACCAGTTGGC
    CGTCCTGGAG AACAAGGTAT CGGTGGTCCA CAAGGTTTGA TTGGAGAGAA GGGTTCATCT
    GGTGAATCTG GTCCTGCTGG TCCTCCTGGA GCTTCTGGTC CTTCTGGTGT CCTTGGTGCT
    CTTGGTATCT CTGGTCTTCC AGGAGCAAGA GGTGAACGTG GTACCCCTGG TGGCCCTGGA
    GCTAATGGTG AGTCAGGTCC TGCTGGCCCT GCAGGTTCTG CTGGTTCCCG TGGCCCCTCT
    GGCCCCGTGG GTTCTCCCGG TCTGACTGGT GTTCATGGTG AAGCTGGTCG TGATGGCAAT
    CCTGGTAATG ATGGTCCTCC CGGTCGTGAT GGTCTTCCTG GAGTTAAGGG AGAACGTGGC
    TATCCTGGAG GCACTGGCCC TGCTGGATCT CTTGGTGCTG TTGGAGCTCC AGGAGCTGTT
    GGTCCTTCTG GAAAATCTGG AAATCGTGGT GAACCTGGTC CCGTTGGTCA TGCTGGAGCT
    GTTGGCCCTG CTGGTCCCAG AGGCCCATCT GGACAACAAG GTACTCGTGG TGACAAGGGT
    GAAGCTGGTG AGAGGGGAGC TAGAGGTTTG GATGGACGCA AAGGACATAA TGGTTTACAA
    GGTCTCCCTG GCCCTGCTGG TTCTCCAGGA GAAACAGGTC CTGCTGGTTC CAACGGTCCA
    TCTGGCCCAC GGGGTCCAGC TGGCCCATCC GGTCCTCCAG GCAAAGAAGG ACGTTCCGGA
    CATAATGGTC CAATTGGTCC AGTCGGTCTT CGTGGTCCAG CTGGTCACCA AGGTCCTGCT
    GGTCCTCCTG GACCACCTGG TCTTCCTGGT CCTGCTGGCG CATCTGGTGG TGGATATGAT
    GGTGGCTTTG GTGGTGAATT CTTCAGAGCT GACCAGCCTG CTCGTAGACC AAAGGATTAT
    GAAGTTGATG CCACTCTGAA ATCTTTGAAC AACCAAATTG AGACAATCCT GACCCCAGAA
    GGCTCAAAGA AGAACCCTGC CCGCACTTGC CGTGACTTAA GACTCAGCCA CCCAGATTGG
    AGCAGTGGAT TCTACTGGAT CGATCCTAAC CAAGGCTGCA CATCTGATTC CATCAGAGTT
    TTCTGTGATT TCTCCACTGG TGAGACCTGC ATCCATGCCA ACCCAGACAG CATTCCACAG
    AAGAACTGGT ACACTAGCAA GACCAAACAA AGGAAGCATG TATGGTTCGG TGAAACCATC
    AATGGTGGCA CACAGATTGA ATACAACGAA GAAGGAGTCA CATCCAAGGA CATGGCCACA
    CAGTTTGCTT TCATGCGTTT ACTGGCTAAT CACGCATCTC AGAATATTAC CTACCACTGC
    AAGAACAGCA TTGCCTATAT GGATGGACAG ACTGGAAACC TGAAGAAGGC CATTTTGCTA
    CAAGGATCCA ATGACGTTGA GCTACGCGCA GAGGGCAACA GCAGATTCAC TTTCAGTGTC
    CTAGAAGATG GTTGCACGCA ACATACGGGT GAATGGGGAA GAACAGTCAT AGAATACAGA
    ACTAACAAAC CATCTCGCTT ACCCATCCTG GACATTGCAC CTTTGGACAT TGGTGGTGAT
    GACCAAGAGT TTGGTCTGGA CATTGGCCCA GTCTGTTTCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29554
    Clone ID Related Accession (Same CDS sequence) XM_012964923.2
    Accession Version XM_012964923.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-2(I) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683410.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAACGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTC CTCCTGGAGC TTCTGGTCCT
    TCTGGTGTCC TTGGTGCTCT TGGTATCTCT GGTCTTCCAG GAGCAAGAGG TGAACGTGGT
    ACCCCTGGTG GCCCTGGAGC TAATGGTGAG TCAGGTCCTG CTGGCCCTGC AGGTTCTGCT
    GGTTCCCGTG GCCCCTCTGG CCCCGTGGGT TCTCCCGGTC TGACTGGTGT TCATGGTGAA
    GCTGGTCGTG ATGGCAATCC TGGTAATGAT GGTCCTCCCG GTCGTGATGG TCTTCCTGGA
    GTTAAGGGAG AACGTGGCTA TCCTGGAGGC ACTGGCCCTG CTGGATCTCT TGGTGCTGTT
    GGAGCTCCAG GAGCTGTTGG TCCTTCTGGA AAATCTGGAA ATCGTGGTGA ACCTGGTCCC
    GTTGGTCATG CTGGAGCTGT TGGCCCTGCT GGTCCCAGAG GCCCATCTGG ACAACAAGGT
    ACTCGTGGTG ACAAGGGTGA AGCTGGTGAG AGGGGAGCTA GAGGTTTGGA TGGACGCAAA
    GGACATAATG GTTTACAAGG TCTCCCTGGC CCTGCTGGTT CTCCAGGAGA AACAGGTCCT
    GCTGGTTCCA ACGGTCCATC TGGCCCACGG GGTCCAGCTG GCCCATCCGG TCCTCCAGGC
    AAAGAAGGAC GTTCCGGACA TAATGGTCCA ATTGGTCCAG TCGGTCTTCG TGGTCCAGCT
    GGTCACCAAG GTCCTGCTGG TCCTCCTGGA CCACCTGGTC TTCCTGGTCC TGCTGGCGCA
    TCTGGTGGTG GATATGATGG TGGCTTTGGT GGTGAATTCT TCAGAGCTGA CCAGCCTGCT
    CGTAGACCAA AGGATTATGA AGTTGATGCC ACTCTGAAAT CTTTGAACAA CCAAATTGAG
    ACAATCCTGA CCCCAGAAGG CTCAAAGAAG AACCCTGCCC GCACTTGCCG TGACTTAAGA
    CTCAGCCACC CAGATTGGAG CAGTGGATTC TACTGGATCG ATCCTAACCA AGGCTGCACA
    TCTGATTCCA TCAGAGTTTT CTGTGATTTC TCCACTGGTG AGACCTGCAT CCATGCCAAC
    CCAGACAGCA TTCCACAGAA GAACTGGTAC ACTAGCAAGA CCAAACAAAG GAAGCATGTA
    TGGTTCGGTG AAACCATCAA TGGTGGCACA CAGATTGAAT ACAACGAAGA AGGAGTCACA
    TCCAAGGACA TGGCCACACA GTTTGCTTTC ATGCGTTTAC TGGCTAATCA CGCATCTCAG
    AATATTACCT ACCACTGCAA GAACAGCATT GCCTATATGG ATGGACAGAC TGGAAACCTG
    AAGAAGGCCA TTTTGCTACA AGGATCCAAT GACGTTGAGC TACGCGCAGA GGGCAACAGC
    AGATTCACTT TCAGTGTCCT AGAAGATGGT TGCACGCAAC ATACGGGTGA ATGGGGAAGA
    ACAGTCATAG AATACAGAAC TAACAAACCA TCTCGCTTAC CCATCCTGGA CATTGCACCT
    TTGGACATTG GTGGTGATGA CCAAGAGTTT GGTCTGGACA TTGGCCCAGT CTGTTTCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820377.1
    CDS147..4049
    Translation

    Target ORF information:

    RefSeq Version XM_012964923.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type 1, alpha 2 (col1a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964923.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAT GCGGACTGTG CTGCTGCTGG CAGTCACACT ATACCTAGCA 
    ACATGCCAAT CTGAGGGGCC CAGAGGAGAC AAAGGCCCAC AGGGAGAGAG GGGCCCACCA
    GGTGCTCCAG GTAGAGATGG TGTTGATGGT GAACCAGGAC TTCCCGGGCC ACCCGGCCCA
    CCCGGACTTG GCGGAAACTT TGCTGCTCAA TATGACCCAT CTAAATCTGG CGGCGGAGAA
    CCTGGCCCTG CGGGTTTAAT GGGACCAAGA GGACCCCCTG GACCACCTGG ATCCCCTGGT
    GCTCAAGGTT TCCAAGGACA TGCTGGTGAA CCTGGCGAAC CTGGCCAACC TGGTCCTTTA
    GGATCTACTG GTCCCTCTGG TCCACCTGGA AAACCCGGTG AAGATGGTCA CTCTGGCAAA
    TCTGGAAGAC CTGGTGAGCG AGGCACAGTT GGATCTCAGG GTGCACGTGG TTTCCCCGGA
    ACTCCTGGTC TCCCTGGATT TAAAGGAATT AGGGGTCACA ACGGTCTGGA TGGTCAGAAG
    GGAGGCCCAG GTGTTGCAGG TGTCAAGGGA GAAACTGGTG CTAATGGTGA AAACGGATCC
    CCTGGTCAGT CTGGTGCACG TGGTCTCCCA GGTGAAAGAG GGCGCGGTGG ACCTCCTGGC
    CCAGCTGGTT CTCGTGGAAG TGATGGCAGT GCAGGCCCCA CCGGCCCAGC TGGTCCTATT
    GGCTCTGCTG GTCCTCCTGG TCTTCCCGGT GCTCCTGGTG CTAAGGGTGA AATAGGATCT
    GCAGGAAGCA CTGGTTCTGC TGGTGCTGCT GGCCCTAGAG GCGAACCTGG TCCACCTGGT
    GCTCTTGGGC CTGTTGGACC TGCTGGAAAT CCCGGTGCTA ACGGAGTAAA TGGAGCTAAA
    GGTGCAGCTG GTCTCCCTGG TGTTGGTGGT GCTCCTGGTT TGCCTGGCCC ACGCGGAATC
    CCAGGTCCCC CAGGACCTTC TGGCCCAGCT GGAGCTAGAG GACTTTCTGG TGATCCTGGT
    GTTGCTGGCG GAAAAGGAGA CACTGGTGCT AAGGGTGAAC CTGGTTCTGC TGGTCCCTCT
    GGTCCTGCTG GTCCATCTGG AGAGGAAGGC AGGAGAGGTC CCAATGGAGA AGCTGGATCA
    TCTGGGCCTG CTGGCAATGC TGGAATTAGA GGTGTTCCCG GAACTCGTGG TCTGCCTGGC
    CCTGATGGTA GAGCTGGTGG AATGGGTGCT CCTGGTAGCC GTGGTGCTTC TGGTCCTGCA
    GGTGCAAGAG GTCCAAATGG TGATGCTGGA CGCCCCGGAG AGCCTGGTCT TCTTGGTGCA
    AGAGGGCTTC CTGGTTTCTC TGGCAGCACT GGACCTGCTG GCAAAGAAGG CCCTGCTGGT
    CCTCAAGGTA TTGAGGGTCG TTCTGGTCCT GCTGGTGCAT CTGGTCCAAG AGGTGAACCT
    GGTAGCATTG GATTCCCTGG TCCCAAGGGT CCAGCTGGTG AGCCTGGAAA GAATGGAGAT
    AAGGGAAGCC AAGGTCCTTC TGGAAACAGG GGAGCCCCGG GACCCGATGG TAACAATGGA
    GCTCAAGGAC CCCCAGGACT TGCTGGTAAT ACAGGAGACA AAGGTGAACA AGGTCCTGCT
    GGTGCCCCAG GCTTCCAGGG TCTTCCAGGC CCCGGTGGCG CTGCTGGAGA ACTTGGCAAA
    CATGGAGAAA GAGGTGCTCC TGGTGACTTT GGCCCTGCTG GCCCAGCTGG TCCAAGAGGT
    GAACGCGGTG CTCCTGGAGA GAGTGGTGCT GCAGGTCCAC TAGGAGCTCT TGGACCCCGA
    GGCCCAACTG GTGCACCTGG ATCTGATGGA GCCAAGGGAG AACCTGGTGC TGCTGGATTA
    AATGGTGCTC TTGGACCATC TGGCCCTGGT GGAATCCCAG GAGAGAGAGG TGCAGCTGGT
    GTTCCTGGAC CAAAGGGAGA AAAGGGTGAC GCTGGCCATT CTGGAGAATA TGGTAACCCA
    GGAAGAGATG GTGCCCGTGG TCCTGCTGGT GCTTCTGGCG CTCCCGGTCC TGCTGGTGCT
    GCTGGTGATA GGGGTGAATC TGGCCCTGCT GGTCCTTCTG GTCTTGCTGG TCCTCGTGGA
    GCTCCTGGTG AACGCGGTGA AGCTGGTCCT GCTGGTTCTA CTGGTTTCGC TGGTCCTCCT
    GGTGCAGCTG GTCACACTGG TGTCAAGGGT GAAAGAGGAC CAAAAGGACC AAAGGGTGAA
    TCTGGTTCTC CTGGTGCTCT CGGTGCAGTT GGTTCCCATG GACCCGCAGG TCCCAATGGC
    CCAGCTGGTA CTACAGGTCC CCGAGGTGAT GGTGGTGCTC CTGGTGCAAA TGGTTTCCCT
    GGTCCTGCTG GAAGAACTGG TGCTCCTGGT CCTGCTGGTC CTCCTGGAGC TTCTGGTCCT
    TCTGGTGTCC TTGGTGCTCT TGGTATCTCT GGTCTTCCAG GAGCAAGAGG TGAACGTGGT
    ACCCCTGGTG GCCCTGGAGC TAATGGTGAG TCAGGTCCTG CTGGCCCTGC AGGTTCTGCT
    GGTTCCCGTG GCCCCTCTGG CCCCGTGGGT TCTCCCGGTC TGACTGGTGT TCATGGTGAA
    GCTGGTCGTG ATGGCAATCC TGGTAATGAT GGTCCTCCCG GTCGTGATGG TCTTCCTGGA
    GTTAAGGGAG AACGTGGCTA TCCTGGAGGC ACTGGCCCTG CTGGATCTCT TGGTGCTGTT
    GGAGCTCCAG GAGCTGTTGG TCCTTCTGGA AAATCTGGAA ATCGTGGTGA ACCTGGTCCC
    GTTGGTCATG CTGGAGCTGT TGGCCCTGCT GGTCCCAGAG GCCCATCTGG ACAACAAGGT
    ACTCGTGGTG ACAAGGGTGA AGCTGGTGAG AGGGGAGCTA GAGGTTTGGA TGGACGCAAA
    GGACATAATG GTTTACAAGG TCTCCCTGGC CCTGCTGGTT CTCCAGGAGA AACAGGTCCT
    GCTGGTTCCA ACGGTCCATC TGGCCCACGG GGTCCAGCTG GCCCATCCGG TCCTCCAGGC
    AAAGAAGGAC GTTCCGGACA TAATGGTCCA ATTGGTCCAG TCGGTCTTCG TGGTCCAGCT
    GGTCACCAAG GTCCTGCTGG TCCTCCTGGA CCACCTGGTC TTCCTGGTCC TGCTGGCGCA
    TCTGGTGGTG GATATGATGG TGGCTTTGGT GGTGAATTCT TCAGAGCTGA CCAGCCTGCT
    CGTAGACCAA AGGATTATGA AGTTGATGCC ACTCTGAAAT CTTTGAACAA CCAAATTGAG
    ACAATCCTGA CCCCAGAAGG CTCAAAGAAG AACCCTGCCC GCACTTGCCG TGACTTAAGA
    CTCAGCCACC CAGATTGGAG CAGTGGATTC TACTGGATCG ATCCTAACCA AGGCTGCACA
    TCTGATTCCA TCAGAGTTTT CTGTGATTTC TCCACTGGTG AGACCTGCAT CCATGCCAAC
    CCAGACAGCA TTCCACAGAA GAACTGGTAC ACTAGCAAGA CCAAACAAAG GAAGCATGTA
    TGGTTCGGTG AAACCATCAA TGGTGGCACA CAGATTGAAT ACAACGAAGA AGGAGTCACA
    TCCAAGGACA TGGCCACACA GTTTGCTTTC ATGCGTTTAC TGGCTAATCA CGCATCTCAG
    AATATTACCT ACCACTGCAA GAACAGCATT GCCTATATGG ATGGACAGAC TGGAAACCTG
    AAGAAGGCCA TTTTGCTACA AGGATCCAAT GACGTTGAGC TACGCGCAGA GGGCAACAGC
    AGATTCACTT TCAGTGTCCT AGAAGATGGT TGCACGCAAC ATACGGGTGA ATGGGGAAGA
    ACAGTCATAG AATACAGAAC TAACAAACCA TCTCGCTTAC CCATCCTGGA CATTGCACCT
    TTGGACATTG GTGGTGATGA CCAAGAGTTT GGTCTGGACA TTGGCCCAGT CTGTTTCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.