COL1A2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL1A2
Entrez Gene ID	396243
Full Name	collagen type I alpha 2 chain
Synonyms	COLA
General protein information	Preferred Names collagen type I alpha 2 chain Names collagen alpha-2(I) chain alpha 2(I) collagen alpha-2 type I collagen alpha-2(1) collagen collagen a2 collagen alpha-2 collagen, type I, alpha 2 precollagen alpha-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079714.2	NP_001073182.2	collagen alpha-2(I) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL1A2	XP_001168894.1
Xenopus tropicalis (tropical clawed frog)	col1a2	NP_001072718.1
Rattus norvegicus (Norway rat)	Col1a2	NP_445808.1
Homo sapiens (human)	COL1A2	NP_000080.2
Mus musculus (house mouse)	Col1a2	NP_031769.2
Gallus gallus (chicken)	COL1A2	NP_001073182.2
	COL1A2	XP_001097831.1
Canis lupus familiaris (dog)	COL1A2	NP_001003187.1
Bos taurus (cattle)	COL1A2	NP_776945.1
Danio rerio (zebrafish)	col1a2	NP_892013.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Bi?mont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guig? R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Qu?tier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H
Nature431(7011)946-57(2004 Oct)

The chick alpha2(I) collagen gene contains two functional promoters, and its expression in chondrocytes is regulated at both transcriptional and post-transcriptional levels.
Pallante KM, Niu Z, Zhao Y, Cohen AJ, Nah HD, Adams SL
The Journal of biological chemistry271(41)25233-9(1996 Oct)

Type V collagen and Bowman's membrane. Quantitation of mRNA in corneal epithelium and stroma.
Gordon MK, Foley JW, Birk DE, Fitch JM, Linsenmayer TF
The Journal of biological chemistry269(40)24959-66(1994 Oct)

Structure of the promoter for chicken alpha 2 type I collagen gene.
Vogeli G, Ohkubo H, Sobel ME, Yamada Y, Pastan I, de Crombrugghe B
Proceedings of the National Academy of Sciences of the United States of America78(9)5334-8(1981 Sep)

The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp.
Yamada Y, Avvedimento VE, Mudryj M, Ohkubo H, Vogeli G, Irani M, Pastan I, de Crombrugghe B
Cell22(3)887-92(1980 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL1A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL1A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00911	NM_001079714.2 Latest version!	Gallus gallus collagen type I alpha 2 chain (COL1A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL1A2 ( NM_001079714.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001073182.2 Gallus gallus collagen type I alpha 2 chain (COL1A2), mRNA.

Price & Availability↑

CloneID	OGa00911
Clone ID Related Accession (Same CDS sequence)	NM_001079714.2
Accession Version	NM_001079714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(I) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ452612.1, DR425078.1, CO504713.1 and AADN05000033.1. On Sep 25, 2008 this sequence version replaced NM_001079714.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

ATGCTCAGCT TTGTGGATAC GCGGATTTTG TTGCTGCTCG CAGTAACTTC ATACCTAGCA 
ACAAGCCAAC ATGTGAGTGA GGCATCTGCA GGGCGGAAGG GCCCTAGAGG AGACAAAGGG 
CCACAGGGAG AAAGGGGTCC ACCAGGTCCA CCAGGCAGAG ATGGTGAAGA CGGTCCACCA 
GGTCCTCCAG GCCCCCCTGG TCCTCCAGGT CTTGGCGGAA ATTTTGCTGC TCAGTATGAT 
CCATCTAAAG CGGCTGACTT TGGCCCCGGA CCTATGGGTT TAATGGGACC TAGAGGCCCA 
CCTGGAGCAT CTGGACCTCC TGGCCCTCCT GGGTTTCAAG GTGTTCCTGG TGAGCCTGGT 
GAACCTGGTC AAACAGGTCC CCAAGGTCCT CGTGGTCCCC CTGGTCCTCC AGGAAAGGCT 
GGTGAAGATG GTCACCCTGG CAAACCTGGA AGACCTGGTG AGAGGGGTGT TGCTGGTCCT 
CAAGGTGCTC GTGGTTTCCC TGGAACTCCT GGTCTGCCTG GCTTTAAGGG AATTAGGGGA 
CACAATGGTC TGGATGGTCA GAAGGGACAA CCTGGTACTC CTGGCACCAA GGGAGAACCT 
GGTGCCCCTG GTGAAAATGG TACCCCAGGA CAACCTGGTG CTCGCGGTCT CCCTGGTGAG 
AGAGGAAGAA TAGGTGCCCC TGGACCAGCT GGTGCTCGTG GGAGTGATGG CAGCGCTGGC 
CCCACTGGAC CTGCAGGCCC CATTGGTGCT GCTGGTCCTC CAGGCTTCCC AGGTGCTCCT 
GGTGCTAAGG GTGAAATCGG ACCTGCTGGT AATGTTGGCC CTACTGGTCC TGCTGGTCCA 
AGAGGAGAGA TTGGACTTCC TGGTTCCAGT GGTCCTGTTG GCCCTCCCGG CAACCCTGGT 
GCTAATGGTC TTCCTGGGGC TAAAGGTGCA GCTGGTCTTC CTGGTGTTGC TGGTGCTCCT 
GGTCTGCCTG GGCCCCGTGG TATTCCTGGT CCTCCTGGCC CTGCTGGTCC AAGTGGTGCT 
AGGGGACTTG TTGGTGAACC AGGCCCTGCT GGTGCCAAGG GAGAAAGTGG TAACAAGGGT 
GAGCCTGGTG CTGCTGGCCC CCCTGGCCCT CCTGGTCCAA GTGGTGAGGA AGGCAAGAGA 
GGCAGCAATG GTGAACCTGG CTCTGCTGGT CCCCCTGGCC CTGCTGGTCT AAGAGGCGTG 
CCTGGATCTC GTGGTCTCCC TGGAGCTGAT GGCAGAGCTG GTGTCATGGG ACCTGCTGGT 
AACCGTGGTG CTAGTGGTCC TGTTGGTGCT AAGGGTCCTA ATGGTGATGC TGGCCGTCCT 
GGTGAGCCTG GTCTTATGGG TCCAAGAGGT CTCCCAGGTC AACCTGGAAG CCCAGGTCCT 
GCTGGCAAAG AAGGTCCTGT TGGTTTCCCT GGAGCAGATG GTAGGGTTGG GCCAATCGGT 
CCAGCTGGTA ATAGAGGTGA ACCTGGCAAC ATTGGATTCC CTGGACCAAA AGGTCCCACT 
GGTGAGCCTG GCAAACCTGG TGAAAAAGGC AATGTTGGTC TTGCTGGCCC ACGGGGTGCT 
CCTGGTCCTG AGGGAAACAA CGGTGCTCAA GGTCCTCCTG GTGTCACTGG CAACCAAGGT 
GCAAAGGGTG AAACAGGTCC TGCTGGCCCT CCTGGCTTCC AGGGTCTGCC AGGTCCCTCC 
GGTCCAGCTG GTGAAGCTGG CAAACCAGGC GAAAGGGGTC TCCATGGTGA ATTTGGTGTC 
CCTGGTCCTG CTGGCCCAAG GGGCGAGCGT GGTCTTCCTG GTGAAAGCGG TGCTGTTGGT 
CCTGCTGGTC CTATTGGAAG CCGTGGTCCA TCTGGCCCAC CAGGCCCTGA TGGTAACAAG 
GGTGAGCCTG GTAATGTCGG TCCTGCTGGT GCTCCTGGTC CCGCTGGGCC TGGTGGAATC 
CCAGGAGAGA GAGGTGTTGC AGGTGTTCCA GGAGGCAAGG GTGAGAAGGG TGCCCCAGGT 
CTGAGAGGTG ACACTGGTGC AACAGGAAGA GATGGTGCCC GTGGTCTTCC TGGTGCTATT 
GGTGCTCCTG GCCCTGCTGG TGGTGCTGGT GATCGGGGTG AAGGAGGTCC TGCTGGTCCC 
GCTGGTCCTG CTGGTGCCCG TGGTATTCCT GGTGAACGTG GTGAGCCTGG TCCTGTAGGT 
CCTAGTGGAT TTGCTGGACC TCCTGGTGCA GCTGGTCAGC CTGGTGCTAA AGGCGAGCGT 
GGCCCTAAGG GACCAAAGGG TGAAACAGGA CCAACAGGTG CTATTGGGCC AATTGGTGCT 
TCTGGACCAC CAGGTCCCGT TGGTGCTGCT GGTCCTGCTG GCCCTCGTGG TGATGCTGGT 
CCTCCTGGCA TGACTGGTTT CCCTGGTGCT GCTGGAAGAG TCGGTCCTCC AGGCCCTGCT 
GGCATCACTG GTCCCCCTGG TCCTCCTGGC CCAGCTGGCA AAGATGGTCC TAGAGGTCTG 
CGTGGTGATG TTGGTCCAGT CGGCCGTACT GGTGAACAGG GTATTGCTGG CCCACCTGGT 
TTTGCTGGTG AGAAAGGTCC ATCTGGAGAG GCTGGTGCTG CTGGTCCTCC CGGTACCCCA 
GGTCCTCAGG GTATTCTTGG TGCTCCTGGT ATCCTTGGTC TGCCTGGCTC TCGGGGAGAA 
CGTGGTCTTC CAGGCATTGC TGGAGCAACA GGTGAACCTG GTCCTCTGGG TGTTTCTGGT 
CCTCCTGGTG CTCGTGGTCC CTCTGGTCCT GTGGGTTCTC CTGGTCCTAA TGGTGCTCCT 
GGTGAAGCTG GTCGTGATGG CAATCCTGGA AATGATGGTC CTCCAGGCCG TGATGGTGCT 
CCTGGCTTCA AGGGTGAGCG TGGTGCTCCT GGTAACCCAG GTCCCAGTGG TGCTTTGGGT 
GCTCCTGGTC CTCATGGCCA AGTTGGTCCT TCTGGAAAGC CTGGAAACCG TGGTGATCCT 
GGTCCTGTTG GTCCTGTTGG TCCTGCTGGT GCTTTTGGCC CAAGAGGTCT CGCTGGCCCA 
CAAGGTCCAC GTGGTGAGAA AGGTGAACCT GGTGATAAGG GACATAGAGG TCTGCCTGGC 
CTGAAGGGAC ACAATGGATT GCAGGGTCTT CCTGGTCTTG CTGGCCAACA TGGTGATCAA 
GGTCCTCCTG GTAACAACGG TCCTGCTGGC CCAAGGGGTC CTCCTGGTCC TTCTGGTCCT 
CCTGGTAAGG ATGGTCGCAA TGGTCTCCCT GGACCCATTG GCCCTGCTGG TGTACGTGGA 
TCTCATGGTA GCCAAGGCCC TGCTGGCCCT CCTGGCCCTC CTGGTCCCCC TGGCCCCCCT 
GGTCCCAATG GTGGCGGATA TGAAGTTGGC TTTGATGCAG AATACTACCG GGCTGATCAG 
CCTTCTCTCA GACCCAAGGA TTATGAAGTT GATGCCACTC TGAAAACATT GAACAACCAA 
ATTGAGACCC TGCTGACCCC AGAAGGCTCC AAAAAGAACC CGGCTCGCAC CTGCCGTGAC 
CTCAGACTTA GCCACCCAGA ATGGAGCAGC GGTTTCTACT GGATTGATCC CAACCAAGGC 
TGCACTGCAG ATGCCATTAG AGCCTACTGT GACTTTGCTA CTGGTGAGAC TTGCATCCAT 
GCTAGCCTTG AAGATATTCC CACTAAGACC TGGTATGTCA GCAAGAACCC CAAGGACAAA 
AAGCACATAT GGTTCGGTGA AACTATCAAT GGTGGTACTC AGTTTGAATA CAATGGTGAA 
GGTGTGACCA CAAAGGACAT GGCCACCCAA CTTGCTTTCA TGCGTCTGCT GGCCAACCAT 
GCCTCTCAGA ACATCACCTA CCACTGCAAG AACAGCATTG CCTACATGGA TGAGGAGACT 
GGAAACCTTA AAAAGGCTGT TATACTCCAG GGATCCAATG ATGTTGAACT ACGAGCTGAA 
GGCAACAGCA GATTCACTTT CAGTGTTCTT GTGGATGGCT GCTCTAAAAA GAACAACAAA 
TGGGGCAAAA CGATCATTGA GTACAGAACA AATAAGCCGT CTCGCTTGCC CATCCTTGAC 
ATTGCACCTT TGGACATTGG TGGCGCTGAC CAAGAATTCG GTTTGCACAT TGGCCCAGTC 
TGTTTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073182.2
CDS	180..4271
Translation	MLSFVDTRILLLLAVTSYLATSQHVSEASAGRKGPRGDKGPQGERGPPGPPGRDGEDGPPGPPGPPGPPGLGGNFAAQYDPSKAADFGPGPMGLMGPRGPPGASGPPGPPGFQGVPGEPGEPGQTGPQGPRGPPGPPGKAGEDGHPGKPGRPGERGVAGPQGARGFPGTPGLPGFKGIRGHNGLDGQKGQPGTPGTKGEPGAPGENGTPGQPGARGLPGERGRIGAPGPAGARGSDGSAGPTGPAGPIGAAGPPGFPGAPGAKGEIGPAGNVGPTGPAGPRGEIGLPGSSGPVGPPGNPGANGLPGAKGAAGLPGVAGAPGLPGPRGIPGPPGPAGPSGARGLVGEPGPAGAKGESGNKGEPGAAGPPGPPGPSGEEGKRGSNGEPGSAGPPGPAGLRGVPGSRGLPGADGRAGVMGPAGNRGASGPVGAKGPNGDAGRPGEPGLMGPRGLPGQPGSPGPAGKEGPVGFPGADGRVGPIGPAGNRGEPGNIGFPGPKGPTGEPGKPGEKGNVGLAGPRGAPGPEGNNGAQGPPGVTGNQGAKGETGPAGPPGFQGLPGPSGPAGEAGKPGERGLHGEFGVPGPAGPRGERGLPGESGAVGPAGPIGSRGPSGPPGPDGNKGEPGNVGPAGAPGPAGPGGIPGERGVAGVPGGKGEKGAPGLRGDTGATGRDGARGLPGAIGAPGPAGGAGDRGEGGPAGPAGPAGARGIPGERGEPGPVGPSGFAGPPGAAGQPGAKGERGPKGPKGETGPTGAIGPIGASGPPGPVGAAGPAGPRGDAGPPGMTGFPGAAGRVGPPGPAGITGPPGPPGPAGKDGPRGLRGDVGPVGRTGEQGIAGPPGFAGEKGPSGEAGAAGPPGTPGPQGILGAPGILGLPGSRGERGLPGIAGATGEPGPLGVSGPPGARGPSGPVGSPGPNGAPGEAGRDGNPGNDGPPGRDGAPGFKGERGAPGNPGPSGALGAPGPHGQVGPSGKPGNRGDPGPVGPVGPAGAFGPRGLAGPQGPRGEKGEPGDKGHRGLPGLKGHNGLQGLPGLAGQHGDQGPPGNNGPAGPRGPPGPSGPPGKDGRNGLPGPIGPAGVRGSHGSQGPAGPPGPPGPPGPPGPNGGGYEVGFDAEYYRADQPSLRPKDYEVDATLKTLNNQIETLLTPEGSKKNPARTCRDLRLSHPEWSSGFYWIDPNQGCTADAIRAYCDFATGETCIHASLEDIPTKTWYVSKNPKDKKHIWFGETINGGTQFEYNGEGVTTKDMATQLAFMRLLANHASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELRAEGNSRFTFSVLVDGCSKKNNKWGKTIIEYRTNKPSRLPILDIAPLDIGGADQEFGLHIGPVCFK

Target ORF information:

RefSeq Version	NM_001079714.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type I alpha 2 chain (COL1A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079714.2

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC GCGGATTTTG TTGCTGCTCG CAGTAACTTC ATACCTAGCA 
ACAAGCCAAC ATGTGAGTGA GGCATCTGCA GGGCGGAAGG GCCCTAGAGG AGACAAAGGG 
CCACAGGGAG AAAGGGGTCC ACCAGGTCCA CCAGGCAGAG ATGGTGAAGA CGGTCCACCA 
GGTCCTCCAG GCCCCCCTGG TCCTCCAGGT CTTGGCGGAA ATTTTGCTGC TCAGTATGAT 
CCATCTAAAG CGGCTGACTT TGGCCCCGGA CCTATGGGTT TAATGGGACC TAGAGGCCCA 
CCTGGAGCAT CTGGACCTCC TGGCCCTCCT GGGTTTCAAG GTGTTCCTGG TGAGCCTGGT 
GAACCTGGTC AAACAGGTCC CCAAGGTCCT CGTGGTCCCC CTGGTCCTCC AGGAAAGGCT 
GGTGAAGATG GTCACCCTGG CAAACCTGGA AGACCTGGTG AGAGGGGTGT TGCTGGTCCT 
CAAGGTGCTC GTGGTTTCCC TGGAACTCCT GGTCTGCCTG GCTTTAAGGG AATTAGGGGA 
CACAATGGTC TGGATGGTCA GAAGGGACAA CCTGGTACTC CTGGCACCAA GGGAGAACCT 
GGTGCCCCTG GTGAAAATGG TACCCCAGGA CAACCTGGTG CTCGCGGTCT CCCTGGTGAG 
AGAGGAAGAA TAGGTGCCCC TGGACCAGCT GGTGCTCGTG GGAGTGATGG CAGCGCTGGC 
CCCACTGGAC CTGCAGGCCC CATTGGTGCT GCTGGTCCTC CAGGCTTCCC AGGTGCTCCT 
GGTGCTAAGG GTGAAATCGG ACCTGCTGGT AATGTTGGCC CTACTGGTCC TGCTGGTCCA 
AGAGGAGAGA TTGGACTTCC TGGTTCCAGT GGTCCTGTTG GCCCTCCCGG CAACCCTGGT 
GCTAATGGTC TTCCTGGGGC TAAAGGTGCA GCTGGTCTTC CTGGTGTTGC TGGTGCTCCT 
GGTCTGCCTG GGCCCCGTGG TATTCCTGGT CCTCCTGGCC CTGCTGGTCC AAGTGGTGCT 
AGGGGACTTG TTGGTGAACC AGGCCCTGCT GGTGCCAAGG GAGAAAGTGG TAACAAGGGT 
GAGCCTGGTG CTGCTGGCCC CCCTGGCCCT CCTGGTCCAA GTGGTGAGGA AGGCAAGAGA 
GGCAGCAATG GTGAACCTGG CTCTGCTGGT CCCCCTGGCC CTGCTGGTCT AAGAGGCGTG 
CCTGGATCTC GTGGTCTCCC TGGAGCTGAT GGCAGAGCTG GTGTCATGGG ACCTGCTGGT 
AACCGTGGTG CTAGTGGTCC TGTTGGTGCT AAGGGTCCTA ATGGTGATGC TGGCCGTCCT 
GGTGAGCCTG GTCTTATGGG TCCAAGAGGT CTCCCAGGTC AACCTGGAAG CCCAGGTCCT 
GCTGGCAAAG AAGGTCCTGT TGGTTTCCCT GGAGCAGATG GTAGGGTTGG GCCAATCGGT 
CCAGCTGGTA ATAGAGGTGA ACCTGGCAAC ATTGGATTCC CTGGACCAAA AGGTCCCACT 
GGTGAGCCTG GCAAACCTGG TGAAAAAGGC AATGTTGGTC TTGCTGGCCC ACGGGGTGCT 
CCTGGTCCTG AGGGAAACAA CGGTGCTCAA GGTCCTCCTG GTGTCACTGG CAACCAAGGT 
GCAAAGGGTG AAACAGGTCC TGCTGGCCCT CCTGGCTTCC AGGGTCTGCC AGGTCCCTCC 
GGTCCAGCTG GTGAAGCTGG CAAACCAGGC GAAAGGGGTC TCCATGGTGA ATTTGGTGTC 
CCTGGTCCTG CTGGCCCAAG GGGCGAGCGT GGTCTTCCTG GTGAAAGCGG TGCTGTTGGT 
CCTGCTGGTC CTATTGGAAG CCGTGGTCCA TCTGGCCCAC CAGGCCCTGA TGGTAACAAG 
GGTGAGCCTG GTAATGTCGG TCCTGCTGGT GCTCCTGGTC CCGCTGGGCC TGGTGGAATC 
CCAGGAGAGA GAGGTGTTGC AGGTGTTCCA GGAGGCAAGG GTGAGAAGGG TGCCCCAGGT 
CTGAGAGGTG ACACTGGTGC AACAGGAAGA GATGGTGCCC GTGGTCTTCC TGGTGCTATT 
GGTGCTCCTG GCCCTGCTGG TGGTGCTGGT GATCGGGGTG AAGGAGGTCC TGCTGGTCCC 
GCTGGTCCTG CTGGTGCCCG TGGTATTCCT GGTGAACGTG GTGAGCCTGG TCCTGTAGGT 
CCTAGTGGAT TTGCTGGACC TCCTGGTGCA GCTGGTCAGC CTGGTGCTAA AGGCGAGCGT 
GGCCCTAAGG GACCAAAGGG TGAAACAGGA CCAACAGGTG CTATTGGGCC AATTGGTGCT 
TCTGGACCAC CAGGTCCCGT TGGTGCTGCT GGTCCTGCTG GCCCTCGTGG TGATGCTGGT 
CCTCCTGGCA TGACTGGTTT CCCTGGTGCT GCTGGAAGAG TCGGTCCTCC AGGCCCTGCT 
GGCATCACTG GTCCCCCTGG TCCTCCTGGC CCAGCTGGCA AAGATGGTCC TAGAGGTCTG 
CGTGGTGATG TTGGTCCAGT CGGCCGTACT GGTGAACAGG GTATTGCTGG CCCACCTGGT 
TTTGCTGGTG AGAAAGGTCC ATCTGGAGAG GCTGGTGCTG CTGGTCCTCC CGGTACCCCA 
GGTCCTCAGG GTATTCTTGG TGCTCCTGGT ATCCTTGGTC TGCCTGGCTC TCGGGGAGAA 
CGTGGTCTTC CAGGCATTGC TGGAGCAACA GGTGAACCTG GTCCTCTGGG TGTTTCTGGT 
CCTCCTGGTG CTCGTGGTCC CTCTGGTCCT GTGGGTTCTC CTGGTCCTAA TGGTGCTCCT 
GGTGAAGCTG GTCGTGATGG CAATCCTGGA AATGATGGTC CTCCAGGCCG TGATGGTGCT 
CCTGGCTTCA AGGGTGAGCG TGGTGCTCCT GGTAACCCAG GTCCCAGTGG TGCTTTGGGT 
GCTCCTGGTC CTCATGGCCA AGTTGGTCCT TCTGGAAAGC CTGGAAACCG TGGTGATCCT 
GGTCCTGTTG GTCCTGTTGG TCCTGCTGGT GCTTTTGGCC CAAGAGGTCT CGCTGGCCCA 
CAAGGTCCAC GTGGTGAGAA AGGTGAACCT GGTGATAAGG GACATAGAGG TCTGCCTGGC 
CTGAAGGGAC ACAATGGATT GCAGGGTCTT CCTGGTCTTG CTGGCCAACA TGGTGATCAA 
GGTCCTCCTG GTAACAACGG TCCTGCTGGC CCAAGGGGTC CTCCTGGTCC TTCTGGTCCT 
CCTGGTAAGG ATGGTCGCAA TGGTCTCCCT GGACCCATTG GCCCTGCTGG TGTACGTGGA 
TCTCATGGTA GCCAAGGCCC TGCTGGCCCT CCTGGCCCTC CTGGTCCCCC TGGCCCCCCT 
GGTCCCAATG GTGGCGGATA TGAAGTTGGC TTTGATGCAG AATACTACCG GGCTGATCAG 
CCTTCTCTCA GACCCAAGGA TTATGAAGTT GATGCCACTC TGAAAACATT GAACAACCAA 
ATTGAGACCC TGCTGACCCC AGAAGGCTCC AAAAAGAACC CGGCTCGCAC CTGCCGTGAC 
CTCAGACTTA GCCACCCAGA ATGGAGCAGC GGTTTCTACT GGATTGATCC CAACCAAGGC 
TGCACTGCAG ATGCCATTAG AGCCTACTGT GACTTTGCTA CTGGTGAGAC TTGCATCCAT 
GCTAGCCTTG AAGATATTCC CACTAAGACC TGGTATGTCA GCAAGAACCC CAAGGACAAA 
AAGCACATAT GGTTCGGTGA AACTATCAAT GGTGGTACTC AGTTTGAATA CAATGGTGAA 
GGTGTGACCA CAAAGGACAT GGCCACCCAA CTTGCTTTCA TGCGTCTGCT GGCCAACCAT 
GCCTCTCAGA ACATCACCTA CCACTGCAAG AACAGCATTG CCTACATGGA TGAGGAGACT 
GGAAACCTTA AAAAGGCTGT TATACTCCAG GGATCCAATG ATGTTGAACT ACGAGCTGAA 
GGCAACAGCA GATTCACTTT CAGTGTTCTT GTGGATGGCT GCTCTAAAAA GAACAACAAA 
TGGGGCAAAA CGATCATTGA GTACAGAACA AATAAGCCGT CTCGCTTGCC CATCCTTGAC 
ATTGCACCTT TGGACATTGG TGGCGCTGAC CAAGAATTCG GTTTGCACAT TGGCCCAGTC 
TGTTTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Nature431(7011)946-57(2004 Oct)
Jaillon O,Aury JM,Brunet F,Petit JL,Stange-Thomann N,Mauceli E,Bouneau L,Fischer C,Ozouf-Costaz C,Bernot A,Nicaud S,Jaffe D,Fisher S,Lutfalla G,Dossat C,Segurens B,Dasilva C,Salanoubat M,Levy M,Boudet N,Castellano S,Anthouard V,Jubin C,Castelli V,Katinka M,Vacherie B,Bi?mont C,Skalli Z,Cattolico L,Poulain J,De Berardinis V,Cruaud C,Duprat S,Brottier P,Coutanceau JP,Gouzy J,Parra G,Lardier G,Chapple C,McKernan KJ,McEwan P,Bosak S,Kellis M,Volff JN,Guig? R,Zody MC,Mesirov J,Lindblad-Toh K,Birren B,Nusbaum C,Kahn D,Robinson-Rechavi M,Laudet V,Schachter V,Qu?tier F,Saurin W,Scarpelli C,Wincker P,Lander ES,Weissenbach J,Roest Crollius H

The chick alpha2(I) collagen gene contains two functional promoters, and its expression in chondrocytes is regulated at both transcriptional and post-transcriptional levels.
The Journal of biological chemistry271(41)25233-9(1996 Oct)
Pallante KM,Niu Z,Zhao Y,Cohen AJ,Nah HD,Adams SL

Type V collagen and Bowman's membrane. Quantitation of mRNA in corneal epithelium and stroma.
The Journal of biological chemistry269(40)24959-66(1994 Oct)
Gordon MK,Foley JW,Birk DE,Fitch JM,Linsenmayer TF

Structure of the promoter for chicken alpha 2 type I collagen gene.
Proceedings of the National Academy of Sciences of the United States of America78(9)5334-8(1981 Sep)
Vogeli G,Ohkubo H,Sobel ME,Yamada Y,Pastan I,de Crombrugghe B

The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp.
Cell22(3)887-92(1980 Dec)
Yamada Y,Avvedimento VE,Mudryj M,Ohkubo H,Vogeli G,i M,Pastan I,de Crombrugghe B

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COL1A2 cDNA ORF clone, Gallus gallus(chicken)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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