col1a2 cDNA ORF clone, Danio rerio(zebrafish)

The following col1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00632 NM_182968.2
Latest version!
Danio rerio collagen, type I, alpha 2 (col1a2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa24381 XM_005159713.4
Latest version!
Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
ODa24381 XM_005159713.3 Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa00632
    Clone ID Related Accession (Same CDS sequence) NM_182968.2
    Accession Version NM_182968.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-2(I) chain
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071278.1. On Jun 16, 2004 this sequence version replaced NM_182968.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071278.1, AJ318213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGTGCC
    GCTGGTCAAG ATGGTCGCAC CGGACCCCCT GGCCCAACTG GACCCAGAGG CCAGCCTGGT
    AACATTGGAT TCCCTGGTCC CAAAGGACCT TCTGGCGAGG CTGGCAAGCC TGGTGAGAAG
    GGACCTGTTG GCCCTACTGG ACTGAGAGGC AGCCCTGGTC CTGATGGTAA CAATGGTCCT
    GCTGGTCCTG TTGGACTTGC TGGTGCCCCT GGTGAGAAAG GAGAGCAGGG ACCCTCTGGT
    GCTCCTGGTT TCCAGGGTCT TCCTGGCCCC GCTGGACCTG TTGGTGAGGC TGGCAAGCCT
    GGTGATAGAG GTATCCCTGG AGATCAAGGT GTATCAGGAC CTGCTGGTGT GAAGGGAGAG
    CGTGGTAACC CTGGACCTGC TGGTGCTGCT GGCGCTCAGG GACCCATTGG TGCTCGTGGA
    CCTTCTGGTA CTCCTGGCCC TGATGGAAAC AAGGGAGAGC CTGGTGCAGT TGGACCTGCT
    GGTGCCCCTG GACCACAGGG AGCTGCTGGT ATGCCTGGTG AGCGTGGTGC TGCTGGCACC
    CCTGGAGCTA AGGGAGAGAA GGGTGAGGCT GGATACAGAG GACTGGAGGG CAATGCTGGA
    AAAGATGGTG CCCGTGGTGC TCCTGGACCC AGTGGACCCC CTGGACCTGC TGGTGCTAAT
    GGTGACAAGG GTGAGACTGG CTCCTTTGGT CCTCCTGGAC CTGCTGGTCC TCGTGGTGCC
    CCTGGTGAGC GTGGTGAGTC TGGCCCTGCT GGACCTTCCG GTTTTGCTGG ACCCCCTGGT
    GCTGATGGCC AGACTGGACC AAGAGGTGAG AAAGGACCTG CTGGTGGAAA GGGTGACGCT
    GGACCCGCTG GCCCTGCCGG TCCCGCTGGA AATACTGGAC CCCTTGGTCC TTCTGGTCCT
    GTTGGCCCAC CTGGTGCCCG TGGTGACAGT GGTCCCACTG GTCTGACTGG CTTCCCTGGT
    GCTCCTGGTA GAGTTGGACC TCCTGGTCCT GCTGGTATTG TTGGACCTGC TGGTCTCACT
    GGTCCCGCTG GAAAGGATGG CCCACGTGGT CCTCGTGGTG ATGTTGGCCC TGCTGGTCCC
    CCAGGAGAGA ATGGAATGAT TGGACCTCTT GGTCTGGCTG GTGAGAAGGG ACCCCCTGGA
    GAGGCTGGTG CACCTGGAGC ACCTGGACCT GCTGGGCCTC AGGGTCAGCT GGGATCACAG
    GGATTCAATG GTCTTCCTGG CTCCAGAGGT GACCGTGGTC TTCCTGGTAT CCCCGGATCA
    GTTGGTGAAC CTGGAAGAGT TGGCCCTGCT GGTGCCCCTG GTGCCCGTGG ACCCGGTGGC
    AACATTGGCA TGCCTGGTAT GACTGGTCCT CAGGGTGAGG CTGGACGTGA GGGAAGCCCT
    GGCAATGATG GACCTCCTGG CCGTCCTGGT GCTGCTGGAA TTAAGGGTGA CCGTGGTGAG
    CCTGGTTCCC CTGGAACTGC TGGACCTGTT GGTGCTCCTG GACCCAATGG ACCATCAGGC
    GCTGTTGGCA GACCTGGAAA CCGTGGTGAA TCTGGTCCTT CTGGTCCTAC TGGCGCTGTT
    GGACCTGCTG GAGCAAGAGG TGCACCTGGC CCTGCTGGAC CTCGTGGTGA GAAGGGTGTT
    GCTGGAGAAA AAGGAGACAG GGGTATGAAG GGACTTCGTG GACATCCTGG TCTGCAGGGA
    ATGCCCGGAC CTAACGGTCC TTCTGGTGAC AGTGGGCCTG CTGGCATTGC TGGACCTTCC
    GGTCCAAGAG GCCCAGCTGG TCCCAATGGC CCCGCTGGTA AAGATGGTTC AAATGGCATG
    CCTGGTGCAA TTGGACCCCC AGGACACCGT GGTCCCGCTG GACATGTTGG ACCTGCTGGT
    CCTCCTGGAT CTCCTGGTCT TCCCGGACCC CCTGGCCCAT CTGGAGGTGG ATATGACACA
    TCTGGAGGCT ATGATGAGTA CAGAGCTGAC CAGGCCTCTC TTAGAGCCAA GGATTACGAG
    GTGGATGCCA CCATCAAGTC CTTGAACACT CAGATTGAGA ATCTGCTTTC CCCTGAGGGC
    TCAAAGAAGA ACCCCGCTCG TACTTGCCGT GACATCAGGC TCAGCCACCC AGAATGGAGC
    AGCGGTTTCT ACTGGATCGA CCCCAACCAG GGCTGCACAA TGGATGCCAT CAAGGCCTTC
    TGCGACTTCT CTACTGGCCA GACCTGCATC CACCCCCACC CAGAGAGCAT CCCACGCAAG
    AACTGGTACA GAAGCTCCCA GGAGAAGAAA CACACCTGGT TTGGCGAGAC TATCAACAGT
    GGTACTGAGT TTGCTTACAA TGACGAGACC CTGAGCCCAC AATCCATGGC TACTCAGCTG
    GCCTTCATGC GTCTACTGGC CAACCAGGCA GTCCAGAACA TCACCTACCA CTGCAAGAAC
    AGCATCGCCT ACATGGATGC TGAGAATGGA AACCTGAAGA AGGCTGTGTT GCTGCAGGGT
    TCCAATGATG TTGAGCTCAG GGCGGAGGGC AACAGCCGCT TCACTTTCAG CGTCCTAGAG
    GATGGCTGCA GTAGACACAC TGGCCAGTGG AGCAAGACAG TCATTGAATA CAGAACAAAT
    AAACCATCTC GCCTTCCCAT CCTCGACATT GCACCTTTGG ACATTGGTGG CGCAGATCAA
    GAGTTTGGTT TGGACATTGG CCCAGTCTGT TTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_892013.2
    CDS179..4237
    Translation

    Target ORF information:

    RefSeq Version NM_182968.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type I, alpha 2 (col1a2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182968.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGTGCC
    GCTGGTCAAG ATGGTCGCAC CGGACCCCCT GGCCCAACTG GACCCAGAGG CCAGCCTGGT
    AACATTGGAT TCCCTGGTCC CAAAGGACCT TCTGGCGAGG CTGGCAAGCC TGGTGAGAAG
    GGACCTGTTG GCCCTACTGG ACTGAGAGGC AGCCCTGGTC CTGATGGTAA CAATGGTCCT
    GCTGGTCCTG TTGGACTTGC TGGTGCCCCT GGTGAGAAAG GAGAGCAGGG ACCCTCTGGT
    GCTCCTGGTT TCCAGGGTCT TCCTGGCCCC GCTGGACCTG TTGGTGAGGC TGGCAAGCCT
    GGTGATAGAG GTATCCCTGG AGATCAAGGT GTATCAGGAC CTGCTGGTGT GAAGGGAGAG
    CGTGGTAACC CTGGACCTGC TGGTGCTGCT GGCGCTCAGG GACCCATTGG TGCTCGTGGA
    CCTTCTGGTA CTCCTGGCCC TGATGGAAAC AAGGGAGAGC CTGGTGCAGT TGGACCTGCT
    GGTGCCCCTG GACCACAGGG AGCTGCTGGT ATGCCTGGTG AGCGTGGTGC TGCTGGCACC
    CCTGGAGCTA AGGGAGAGAA GGGTGAGGCT GGATACAGAG GACTGGAGGG CAATGCTGGA
    AAAGATGGTG CCCGTGGTGC TCCTGGACCC AGTGGACCCC CTGGACCTGC TGGTGCTAAT
    GGTGACAAGG GTGAGACTGG CTCCTTTGGT CCTCCTGGAC CTGCTGGTCC TCGTGGTGCC
    CCTGGTGAGC GTGGTGAGTC TGGCCCTGCT GGACCTTCCG GTTTTGCTGG ACCCCCTGGT
    GCTGATGGCC AGACTGGACC AAGAGGTGAG AAAGGACCTG CTGGTGGAAA GGGTGACGCT
    GGACCCGCTG GCCCTGCCGG TCCCGCTGGA AATACTGGAC CCCTTGGTCC TTCTGGTCCT
    GTTGGCCCAC CTGGTGCCCG TGGTGACAGT GGTCCCACTG GTCTGACTGG CTTCCCTGGT
    GCTCCTGGTA GAGTTGGACC TCCTGGTCCT GCTGGTATTG TTGGACCTGC TGGTCTCACT
    GGTCCCGCTG GAAAGGATGG CCCACGTGGT CCTCGTGGTG ATGTTGGCCC TGCTGGTCCC
    CCAGGAGAGA ATGGAATGAT TGGACCTCTT GGTCTGGCTG GTGAGAAGGG ACCCCCTGGA
    GAGGCTGGTG CACCTGGAGC ACCTGGACCT GCTGGGCCTC AGGGTCAGCT GGGATCACAG
    GGATTCAATG GTCTTCCTGG CTCCAGAGGT GACCGTGGTC TTCCTGGTAT CCCCGGATCA
    GTTGGTGAAC CTGGAAGAGT TGGCCCTGCT GGTGCCCCTG GTGCCCGTGG ACCCGGTGGC
    AACATTGGCA TGCCTGGTAT GACTGGTCCT CAGGGTGAGG CTGGACGTGA GGGAAGCCCT
    GGCAATGATG GACCTCCTGG CCGTCCTGGT GCTGCTGGAA TTAAGGGTGA CCGTGGTGAG
    CCTGGTTCCC CTGGAACTGC TGGACCTGTT GGTGCTCCTG GACCCAATGG ACCATCAGGC
    GCTGTTGGCA GACCTGGAAA CCGTGGTGAA TCTGGTCCTT CTGGTCCTAC TGGCGCTGTT
    GGACCTGCTG GAGCAAGAGG TGCACCTGGC CCTGCTGGAC CTCGTGGTGA GAAGGGTGTT
    GCTGGAGAAA AAGGAGACAG GGGTATGAAG GGACTTCGTG GACATCCTGG TCTGCAGGGA
    ATGCCCGGAC CTAACGGTCC TTCTGGTGAC AGTGGGCCTG CTGGCATTGC TGGACCTTCC
    GGTCCAAGAG GCCCAGCTGG TCCCAATGGC CCCGCTGGTA AAGATGGTTC AAATGGCATG
    CCTGGTGCAA TTGGACCCCC AGGACACCGT GGTCCCGCTG GACATGTTGG ACCTGCTGGT
    CCTCCTGGAT CTCCTGGTCT TCCCGGACCC CCTGGCCCAT CTGGAGGTGG ATATGACACA
    TCTGGAGGCT ATGATGAGTA CAGAGCTGAC CAGGCCTCTC TTAGAGCCAA GGATTACGAG
    GTGGATGCCA CCATCAAGTC CTTGAACACT CAGATTGAGA ATCTGCTTTC CCCTGAGGGC
    TCAAAGAAGA ACCCCGCTCG TACTTGCCGT GACATCAGGC TCAGCCACCC AGAATGGAGC
    AGCGGTTTCT ACTGGATCGA CCCCAACCAG GGCTGCACAA TGGATGCCAT CAAGGCCTTC
    TGCGACTTCT CTACTGGCCA GACCTGCATC CACCCCCACC CAGAGAGCAT CCCACGCAAG
    AACTGGTACA GAAGCTCCCA GGAGAAGAAA CACACCTGGT TTGGCGAGAC TATCAACAGT
    GGTACTGAGT TTGCTTACAA TGACGAGACC CTGAGCCCAC AATCCATGGC TACTCAGCTG
    GCCTTCATGC GTCTACTGGC CAACCAGGCA GTCCAGAACA TCACCTACCA CTGCAAGAAC
    AGCATCGCCT ACATGGATGC TGAGAATGGA AACCTGAAGA AGGCTGTGTT GCTGCAGGGT
    TCCAATGATG TTGAGCTCAG GGCGGAGGGC AACAGCCGCT TCACTTTCAG CGTCCTAGAG
    GATGGCTGCA GTAGACACAC TGGCCAGTGG AGCAAGACAG TCATTGAATA CAGAACAAAT
    AAACCATCTC GCCTTCCCAT CCTCGACATT GCACCTTTGG ACATTGGTGG CGCAGATCAA
    GAGTTTGGTT TGGACATTGG CCCAGTCTGT TTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24381
    Clone ID Related Accession (Same CDS sequence) XM_005159713.3 , XM_005159713.4
    Accession Version XM_005159713.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-2(I) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG
    GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT
    CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA
    GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT
    GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA
    CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG
    GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG
    CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT
    GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA
    GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC
    CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA
    CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC
    GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT
    GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA
    CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT
    GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT
    GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT
    GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT
    GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT
    CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT
    CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT
    GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG
    GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA
    ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT
    GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT
    TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA
    CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT
    GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT
    GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT
    AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT
    GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA
    TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT
    CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG
    AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG
    CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA
    ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC
    CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG
    TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA
    CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC
    ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG
    AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC
    TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA
    GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG
    GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159770.1
    CDS188..4147
    Translation

    Target ORF information:

    RefSeq Version XM_005159713.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG
    GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT
    CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA
    GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT
    GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA
    CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG
    GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG
    CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT
    GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA
    GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC
    CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA
    CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC
    GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT
    GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA
    CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT
    GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT
    GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT
    GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT
    GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT
    CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT
    CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT
    GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG
    GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA
    ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT
    GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT
    TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA
    CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT
    GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT
    GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT
    AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT
    GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA
    TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT
    CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG
    AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG
    CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA
    ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC
    CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG
    TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA
    CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC
    ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG
    AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC
    TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA
    GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG
    GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa24381
    Clone ID Related Accession (Same CDS sequence) XM_005159713.3 , XM_005159713.4
    Accession Version XM_005159713.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-2(I) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG
    GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT
    CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA
    GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT
    GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA
    CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG
    GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG
    CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT
    GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA
    GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC
    CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA
    CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC
    GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT
    GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA
    CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT
    GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT
    GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT
    GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT
    GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT
    CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT
    CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT
    GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG
    GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA
    ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT
    GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT
    TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA
    CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT
    GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT
    GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT
    AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT
    GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA
    TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT
    CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG
    AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG
    CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA
    ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC
    CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG
    TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA
    CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC
    ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG
    AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC
    TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA
    GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG
    GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159770.1
    CDS182..4141
    Translation

    Target ORF information:

    RefSeq Version XM_005159713.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159713.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
    TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA
    CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT
    GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT
    GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT
    CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT
    GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT
    GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT
    CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA
    GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC
    GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA
    GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC
    TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT
    GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC
    GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA
    GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT
    GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT
    GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG
    CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT
    GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG
    GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA
    GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA
    GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG
    GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT
    CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA
    GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT
    GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA
    CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG
    GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG
    CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT
    GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA
    GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC
    CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA
    CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC
    GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT
    GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA
    CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT
    GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT
    GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT
    GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT
    GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT
    CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT
    CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT
    GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG
    GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA
    ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT
    GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT
    TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA
    CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT
    GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT
    GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT
    AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT
    GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA
    TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT
    CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG
    AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG
    CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA
    ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC
    CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG
    TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA
    CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC
    ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG
    AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC
    TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA
    GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG
    GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.