COL1A2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL1A2
Entrez Gene ID	282188
Full Name	collagen type I alpha 2 chain
General protein information	Preferred Names collagen type I alpha 2 chain Names collagen alpha-2(I) chain alpha-2 type I collagen collagen 1 alpha 2 chain collagen, type I, alpha 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174520.2	NP_776945.1	collagen alpha-2(I) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL1A2	XP_001168894.1
Xenopus tropicalis (tropical clawed frog)	col1a2	NP_001072718.1
Rattus norvegicus (Norway rat)	Col1a2	NP_445808.1
Homo sapiens (human)	COL1A2	NP_000080.2
Mus musculus (house mouse)	Col1a2	NP_031769.2
Gallus gallus (chicken)	COL1A2	NP_001073182.2
	COL1A2	XP_001097831.1
Canis lupus familiaris (dog)	COL1A2	NP_001003187.1
Bos taurus (cattle)	COL1A2	NP_776945.1
Danio rerio (zebrafish)	col1a2	NP_892013.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Matrix-specific suppression of integrin activation in shear stress signaling.
Orr AW, Ginsberg MH, Shattil SJ, Deckmyn H, Schwartz MA
Molecular biology of the cell17(11)4686-97(2006 Nov)

Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells.
Wangoo A, Johnson L, Gough J, Ackbar R, Inglut S, Hicks D, Spencer Y, Hewinson G, Vordermeier M
Journal of comparative pathology133(4)223-34(2005 Nov)

The FAXWXXT motif in the carboxyl terminus of Vibrio mimicus metalloprotease is involved in binding to collagen.
Lee JH, Ahn SH, Lee EM, Jeong SH, Kim YO, Lee SJ, Kong IS
FEBS letters579(11)2507-13(2005 Apr)

Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors.
Magklara A, Mellati AA, Wasney GA, Little SP, Sotiropoulou G, Becker GW, Diamandis EP
Biochemical and biophysical research communications307(4)948-55(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Flow induced alpha2beta1 activation in cells on collagen, but not on fibronectin or fibrinogen. Conversely, alpha5beta1 and alphavbeta3 are activated on fibronectin and fibrinogen, but not collagen.
Title: Matrix-specific suppression of integrin activation in shear stress signaling.

In advanced stage granulomas in Mycobacterium bovis-infected cattle, there was an increase in the expression of type I procollagen
Title: Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells.

The following COL1A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL1A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41435	NM_174520.2 Latest version!	Bos taurus collagen type I alpha 2 chain (COL1A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL1A2 ( NM_174520.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776945.1 Bos taurus collagen type I alpha 2 chain (COL1A2), mRNA.

Price & Availability↑

CloneID	OBa41435
Clone ID Related Accession (Same CDS sequence)	NM_174520.2
Accession Version	NM_174520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	collagen alpha-2(I) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB008683.1. On Jun 3, 2003 this sequence version replaced NM_174520.1. ##Evidence-Data-START## Transcript exon combination :: AB008683.1, BC149095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGCTCAGCT TTGTGGATAC GCGGACTTTG TTGCTGCTTG CAGTAACTTC GTGCCTAGCA 
ACATGCCAAT CCTTACAAGA GGCAACTGCA AGAAAGGGCC CAAGTGGAGA TAGAGGACCA 
CGCGGAGAAA GGGGTCCACC AGGCCCACCA GGCAGAGATG GTGATGACGG CATCCCAGGC 
CCTCCTGGCC CCCCTGGCCC TCCTGGCCCC CCTGGTCTTG GCGGGAACTT TGCTGCTCAG 
TTTGATGCAA AAGGAGGTGG CCCTGGACCA ATGGGGCTGA TGGGACCTCG CGGCCCTCCT 
GGGGCTTCTG GAGCCCCTGG CCCTCAAGGT TTCCAGGGAC CTCCGGGTGA GCCTGGTGAA 
CCTGGTCAGA CTGGTCCTGC AGGTGCTCGT GGCCCGCCTG GCCCTCCTGG CAAGGCTGGT 
GAGGATGGTC ACCCTGGAAA ACCTGGACGA CCTGGTGAGA GAGGGGTTGT TGGACCACAG 
GGTGCTCGTG GCTTTCCTGG AACTCCTGGA CTCCCTGGCT TCAAGGGCAT TAGGGGTCAC 
AATGGTCTGG ATGGATTGAA GGGACAGCCT GGTGCTCCAG GTGTGAAGGG TGAACCTGGT 
GCCCCTGGTG AAAATGGAAC TCCAGGTCAA ACGGGAGCCC GTGGTCTTCC TGGTGAGAGA 
GGACGTGTTG GTGCCCCTGG CCCAGCTGGT GCCCGTGGAA GTGATGGAAG TGTGGGTCCT 
GTGGGCCCTG CTGGTCCCAT TGGGTCTGCT GGCCCTCCAG GCTTCCCAGG TGCTCCTGGC 
CCCAAGGGTG AACTCGGACC TGTTGGTAAC CCTGGCCCTG CTGGTCCCGC GGGTCCCCGT 
GGTGAAGTGG GTCTCCCAGG CCTTTCTGGC CCTGTCGGAC CTCCTGGAAA CCCCGGAGCC 
AATGGGCTTC CTGGCGCTAA GGGTGCTGCT GGCCTTCCCG GTGTTGCTGG GGCTCCCGGC 
CTCCCTGGAC CCCGGGGTAT TCCTGGCCCT GTTGGCGCTG CTGGTGCTAC TGGCGCCAGA 
GGACTTGTTG GTGAGCCCGG CCCAGCTGGT TCGAAAGGAG AGAGCGGCAA CAAGGGCGAG 
CCTGGTGCTG TTGGGCAGCC AGGTCCTCCT GGCCCCAGTG GTGAAGAAGG AAAGAGAGGC 
TCCACTGGAG AAATCGGACC CGCTGGCCCC CCAGGACCTC CTGGGCTGAG GGGAAATCCT 
GGCTCCCGTG GTCTACCTGG AGCTGACGGC AGAGCTGGTG TCATGGGTCC TGCTGGTAGC 
CGTGGTGCAA CTGGCCCTGC TGGTGTGCGA GGTCCCAATG GAGATTCTGG TCGCCCTGGA 
GAGCCTGGCC TCATGGGACC CCGAGGTTTC CCAGGTTCCC CTGGAAATAT CGGCCCAGCT 
GGTAAAGAAG GTCCTGTGGG TCTCCCTGGT ATTGACGGCA GACCTGGGCC CATTGGCCCA 
GCGGGAGCAA GAGGAGAGCC TGGCAACATT GGATTCCCTG GACCCAAAGG CCCCAGTGGT 
GATCCTGGCA AAGCTGGTGA AAAAGGTCAT GCTGGTCTTG CTGGTGCTCG GGGCGCTCCA 
GGTCCCGATG GCAACAACGG TGCTCAGGGA CCCCCTGGAC TACAGGGTGT CCAAGGTGGA 
AAAGGTGAAC AGGGTCCTGC TGGTCCTCCA GGCTTCCAGG GTCTGCCTGG CCCTGCAGGC 
ACAGCTGGTG AAGCTGGCAA ACCAGGAGAA AGGGGTATCC CTGGTGAATT TGGTCTCCCT 
GGCCCTGCTG GTGCAAGAGG GGAGCGGGGG CCCCCAGGTG AAAGTGGTGC TGCTGGGCCT 
ACTGGGCCTA TTGGAAGCCG AGGTCCTTCT GGACCCCCAG GGCCTGATGG AAACAAGGGT 
GAACCGGGTG TGGTTGGCGC TCCAGGCACT GCTGGCCCAT CTGGTCCTAG CGGACTCCCA 
GGAGAGAGGG GTGCGGCTGG CATTCCTGGA GGCAAGGGAG AAAAGGGTGA AACTGGTCTC 
AGAGGTGACA TTGGTAGCCC TGGTAGAGAT GGTGCTCGTG GTGCTCCTGG TGCTATTGGT 
GCTCCTGGCC CTGCTGGAGC CAATGGGGAC CGGGGTGAAG CTGGTCCCGC TGGCCCTGCT 
GGCCCTGCTG GTCCTCGTGG TAGCCCTGGT GAACGTGGTG AGGTCGGTCC CGCTGGCCCC 
AACGGATTTG CTGGTCCTGC TGGTGCTGCT GGTCAACCTG GTGCTAAAGG AGAGAGAGGA 
ACCAAAGGAC CCAAGGGTGA AAATGGTCCT GTTGGTCCCA CAGGCCCCGT TGGAGCTGCC 
GGTCCGTCTG GTCCAAATGG CCCACCTGGT CCTGCTGGAA GTCGTGGTGA TGGAGGGCCC 
CCTGGGGCTA CTGGTTTCCC TGGTGCTGCT GGACGGACTG GTCCCCCTGG ACCCTCTGGT 
ATCTCTGGCC CCCCTGGCCC CCCTGGTCCT GCTGGTAAAG AAGGGCTTCG TGGGCCTCGT 
GGTGACCAAG GTCCAGTTGG TCGAAGTGGA GAGACAGGTG CCTCTGGCCC TCCTGGCTTT 
GTTGGTGAGA AGGGTCCCTC TGGAGAGCCT GGTACTGCTG GGCCTCCTGG AACCCCAGGT 
CCACAAGGCC TTCTTGGTGC TCCTGGTTTT CTGGGTCTCC CAGGCTCTAG AGGTGAGCGT 
GGTCTACCAG GTGTCGCTGG ATCTGTGGGT GAACCTGGCC CCCTCGGCAT CGCAGGCCCA 
CCTGGGGCCC GTGGTCCCCC TGGTAATGTC GGTAATCCTG GCGTCAATGG TGCTCCTGGT 
GAAGCCGGTC GTGACGGCAA CCCTGGGAAT GACGGTCCCC CAGGCCGCGA TGGTCAACCC 
GGACACAAGG GGGAGCGTGG TTACCCCGGT AACGCAGGTC CTGTTGGTGC TGCCGGTGCT 
CCTGGCCCTC AAGGCCCTGT GGGTCCCGTT GGTAAACACG GAAACCGTGG TGAACCGGGT 
CCTGCCGGTG CTGTTGGTCC TGCTGGTGCC GTTGGCCCAA GAGGTCCCAG TGGCCCACAA 
GGTATTCGAG GTGACAAGGG AGAGCCTGGT GATAAGGGTC CCAGAGGTCT TCCTGGCTTA 
AAGGGACACA ATGGGTTGCA AGGTCTCCCG GGTCTTGCTG GTCATCATGG CGATCAAGGT 
GCTCCCGGTG CTGTGGGTCC CGCTGGTCCC AGGGGCCCTG CTGGTCCTTC TGGCCCCGCT 
GGCAAAGACG GTCGCATTGG ACAGCCTGGT GCAGTCGGAC CTGCTGGCAT TCGTGGCTCT 
CAGGGTAGCC AAGGTCCTGC TGGCCCTCCT GGTCCCCCTG GCCCTCCTGG ACCTCCTGGC 
CCAAGTGGTG GTGGTTACGA GTTTGGTTTT GATGGAGACT TCTACAGGGC TGACCAGCCT 
CGCTCACCAA CTTCTCTCAG ACCCAAGGAT TATGAAGTTG ATGCTACTCT GAAATCTCTC 
AACAACCAGA TTGAGACCCT TCTTACTCCA GAAGGCTCTA GGAAGAACCC AGCTCGCACA 
TGCCGAGACT TGAGACTCAG CCACCCAGAA TGGAGCAGTG GTTACTACTG GATTGACCCT 
AACCAAGGAT GTACTATGGA TGCTATCAAA GTATACTGTG ATTTCTCTAC TGGCGAAACC 
TGCATCCGGG CTCAACCTGA AGACATCCCA GTCAAGAACT GGTACAGAAA TTCCAAGGCC 
AAGAAGCATG TCTGGGTAGG AGAAACTATC AACGGTGGTA CCCAGTTTGA ATATAATGTT 
GAAGGAGTAA CCACCAAGGA AATGGCTACC CAACTTGCCT TCATGCGTCT GCTGGCCAAC 
CATGCCTCTC AGAACATCAC CTACCATTGC AAGAACAGCA TTGCATACAT GGATGAGGAA 
ACTGGCAACC TGAAAAAGGC TGTCATTCTG CAAGGATCCA ATGATGTCGA ACTTGTTGCC 
GAGGGCAACA GCAGATTCAC TTACACTGTT CTTGTAGATG GCTGCTCTAA AAAGACAAAT 
GAATGGCAGA AGACAATCAT TGAATATAAA ACAAACAAGC CATCTCGCCT GCCTATCCTT 
GATATTGCAC CTTTGGACAT CGGTGGCGCT GACCAAGAAA TCAGATTGAA CATTGGCCCA 
GTCTGTTTCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776945.1
CDS	111..4205
Translation	MLSFVDTRTLLLLAVTSCLATCQSLQEATARKGPSGDRGPRGERGPPGPPGRDGDDGIPGPPGPPGPPGPPGLGGNFAAQFDAKGGGPGPMGLMGPRGPPGASGAPGPQGFQGPPGEPGEPGQTGPAGARGPPGPPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGENGTPGQTGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPGPAGPAGPRGEVGLPGLSGPVGPPGNPGANGLPGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATGARGLVGEPGPAGSKGESGNKGEPGAVGQPGPPGPSGEEGKRGSTGEIGPAGPPGPPGLRGNPGSRGLPGADGRAGVMGPAGSRGATGPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPSGDPGKAGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGLPGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGTKGPKGENGPVGPTGPVGAAGPSGPNGPPGPAGSRGDGGPPGATGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRSGETGASGPPGFVGEKGPSGEPGTAGPPGTPGPQGLLGAPGFLGLPGSRGERGLPGVAGSVGEPGPLGIAGPPGARGPPGNVGNPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNAGPVGAAGAPGPQGPVGPVGKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGPAGPRGPAGPSGPAGKDGRIGQPGAVGPAGIRGSQGSQGPAGPPGPPGPPGPPGPSGGGYEFGFDGDFYRADQPRSPTSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTGETCIRAQPEDIPVKNWYRNSKAKKHVWVGETINGGTQFEYNVEGVTTKEMATQLAFMRLLANHASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEWQKTIIEYKTNKPSRLPILDIAPLDIGGADQEIRLNIGPVCFK

Target ORF information:

RefSeq Version	NM_174520.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type I alpha 2 chain (COL1A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174520.2

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC GCGGACTTTG TTGCTGCTTG CAGTAACTTC GTGCCTAGCA 
ACATGCCAAT CCTTACAAGA GGCAACTGCA AGAAAGGGCC CAAGTGGAGA TAGAGGACCA 
CGCGGAGAAA GGGGTCCACC AGGCCCACCA GGCAGAGATG GTGATGACGG CATCCCAGGC 
CCTCCTGGCC CCCCTGGCCC TCCTGGCCCC CCTGGTCTTG GCGGGAACTT TGCTGCTCAG 
TTTGATGCAA AAGGAGGTGG CCCTGGACCA ATGGGGCTGA TGGGACCTCG CGGCCCTCCT 
GGGGCTTCTG GAGCCCCTGG CCCTCAAGGT TTCCAGGGAC CTCCGGGTGA GCCTGGTGAA 
CCTGGTCAGA CTGGTCCTGC AGGTGCTCGT GGCCCGCCTG GCCCTCCTGG CAAGGCTGGT 
GAGGATGGTC ACCCTGGAAA ACCTGGACGA CCTGGTGAGA GAGGGGTTGT TGGACCACAG 
GGTGCTCGTG GCTTTCCTGG AACTCCTGGA CTCCCTGGCT TCAAGGGCAT TAGGGGTCAC 
AATGGTCTGG ATGGATTGAA GGGACAGCCT GGTGCTCCAG GTGTGAAGGG TGAACCTGGT 
GCCCCTGGTG AAAATGGAAC TCCAGGTCAA ACGGGAGCCC GTGGTCTTCC TGGTGAGAGA 
GGACGTGTTG GTGCCCCTGG CCCAGCTGGT GCCCGTGGAA GTGATGGAAG TGTGGGTCCT 
GTGGGCCCTG CTGGTCCCAT TGGGTCTGCT GGCCCTCCAG GCTTCCCAGG TGCTCCTGGC 
CCCAAGGGTG AACTCGGACC TGTTGGTAAC CCTGGCCCTG CTGGTCCCGC GGGTCCCCGT 
GGTGAAGTGG GTCTCCCAGG CCTTTCTGGC CCTGTCGGAC CTCCTGGAAA CCCCGGAGCC 
AATGGGCTTC CTGGCGCTAA GGGTGCTGCT GGCCTTCCCG GTGTTGCTGG GGCTCCCGGC 
CTCCCTGGAC CCCGGGGTAT TCCTGGCCCT GTTGGCGCTG CTGGTGCTAC TGGCGCCAGA 
GGACTTGTTG GTGAGCCCGG CCCAGCTGGT TCGAAAGGAG AGAGCGGCAA CAAGGGCGAG 
CCTGGTGCTG TTGGGCAGCC AGGTCCTCCT GGCCCCAGTG GTGAAGAAGG AAAGAGAGGC 
TCCACTGGAG AAATCGGACC CGCTGGCCCC CCAGGACCTC CTGGGCTGAG GGGAAATCCT 
GGCTCCCGTG GTCTACCTGG AGCTGACGGC AGAGCTGGTG TCATGGGTCC TGCTGGTAGC 
CGTGGTGCAA CTGGCCCTGC TGGTGTGCGA GGTCCCAATG GAGATTCTGG TCGCCCTGGA 
GAGCCTGGCC TCATGGGACC CCGAGGTTTC CCAGGTTCCC CTGGAAATAT CGGCCCAGCT 
GGTAAAGAAG GTCCTGTGGG TCTCCCTGGT ATTGACGGCA GACCTGGGCC CATTGGCCCA 
GCGGGAGCAA GAGGAGAGCC TGGCAACATT GGATTCCCTG GACCCAAAGG CCCCAGTGGT 
GATCCTGGCA AAGCTGGTGA AAAAGGTCAT GCTGGTCTTG CTGGTGCTCG GGGCGCTCCA 
GGTCCCGATG GCAACAACGG TGCTCAGGGA CCCCCTGGAC TACAGGGTGT CCAAGGTGGA 
AAAGGTGAAC AGGGTCCTGC TGGTCCTCCA GGCTTCCAGG GTCTGCCTGG CCCTGCAGGC 
ACAGCTGGTG AAGCTGGCAA ACCAGGAGAA AGGGGTATCC CTGGTGAATT TGGTCTCCCT 
GGCCCTGCTG GTGCAAGAGG GGAGCGGGGG CCCCCAGGTG AAAGTGGTGC TGCTGGGCCT 
ACTGGGCCTA TTGGAAGCCG AGGTCCTTCT GGACCCCCAG GGCCTGATGG AAACAAGGGT 
GAACCGGGTG TGGTTGGCGC TCCAGGCACT GCTGGCCCAT CTGGTCCTAG CGGACTCCCA 
GGAGAGAGGG GTGCGGCTGG CATTCCTGGA GGCAAGGGAG AAAAGGGTGA AACTGGTCTC 
AGAGGTGACA TTGGTAGCCC TGGTAGAGAT GGTGCTCGTG GTGCTCCTGG TGCTATTGGT 
GCTCCTGGCC CTGCTGGAGC CAATGGGGAC CGGGGTGAAG CTGGTCCCGC TGGCCCTGCT 
GGCCCTGCTG GTCCTCGTGG TAGCCCTGGT GAACGTGGTG AGGTCGGTCC CGCTGGCCCC 
AACGGATTTG CTGGTCCTGC TGGTGCTGCT GGTCAACCTG GTGCTAAAGG AGAGAGAGGA 
ACCAAAGGAC CCAAGGGTGA AAATGGTCCT GTTGGTCCCA CAGGCCCCGT TGGAGCTGCC 
GGTCCGTCTG GTCCAAATGG CCCACCTGGT CCTGCTGGAA GTCGTGGTGA TGGAGGGCCC 
CCTGGGGCTA CTGGTTTCCC TGGTGCTGCT GGACGGACTG GTCCCCCTGG ACCCTCTGGT 
ATCTCTGGCC CCCCTGGCCC CCCTGGTCCT GCTGGTAAAG AAGGGCTTCG TGGGCCTCGT 
GGTGACCAAG GTCCAGTTGG TCGAAGTGGA GAGACAGGTG CCTCTGGCCC TCCTGGCTTT 
GTTGGTGAGA AGGGTCCCTC TGGAGAGCCT GGTACTGCTG GGCCTCCTGG AACCCCAGGT 
CCACAAGGCC TTCTTGGTGC TCCTGGTTTT CTGGGTCTCC CAGGCTCTAG AGGTGAGCGT 
GGTCTACCAG GTGTCGCTGG ATCTGTGGGT GAACCTGGCC CCCTCGGCAT CGCAGGCCCA 
CCTGGGGCCC GTGGTCCCCC TGGTAATGTC GGTAATCCTG GCGTCAATGG TGCTCCTGGT 
GAAGCCGGTC GTGACGGCAA CCCTGGGAAT GACGGTCCCC CAGGCCGCGA TGGTCAACCC 
GGACACAAGG GGGAGCGTGG TTACCCCGGT AACGCAGGTC CTGTTGGTGC TGCCGGTGCT 
CCTGGCCCTC AAGGCCCTGT GGGTCCCGTT GGTAAACACG GAAACCGTGG TGAACCGGGT 
CCTGCCGGTG CTGTTGGTCC TGCTGGTGCC GTTGGCCCAA GAGGTCCCAG TGGCCCACAA 
GGTATTCGAG GTGACAAGGG AGAGCCTGGT GATAAGGGTC CCAGAGGTCT TCCTGGCTTA 
AAGGGACACA ATGGGTTGCA AGGTCTCCCG GGTCTTGCTG GTCATCATGG CGATCAAGGT 
GCTCCCGGTG CTGTGGGTCC CGCTGGTCCC AGGGGCCCTG CTGGTCCTTC TGGCCCCGCT 
GGCAAAGACG GTCGCATTGG ACAGCCTGGT GCAGTCGGAC CTGCTGGCAT TCGTGGCTCT 
CAGGGTAGCC AAGGTCCTGC TGGCCCTCCT GGTCCCCCTG GCCCTCCTGG ACCTCCTGGC 
CCAAGTGGTG GTGGTTACGA GTTTGGTTTT GATGGAGACT TCTACAGGGC TGACCAGCCT 
CGCTCACCAA CTTCTCTCAG ACCCAAGGAT TATGAAGTTG ATGCTACTCT GAAATCTCTC 
AACAACCAGA TTGAGACCCT TCTTACTCCA GAAGGCTCTA GGAAGAACCC AGCTCGCACA 
TGCCGAGACT TGAGACTCAG CCACCCAGAA TGGAGCAGTG GTTACTACTG GATTGACCCT 
AACCAAGGAT GTACTATGGA TGCTATCAAA GTATACTGTG ATTTCTCTAC TGGCGAAACC 
TGCATCCGGG CTCAACCTGA AGACATCCCA GTCAAGAACT GGTACAGAAA TTCCAAGGCC 
AAGAAGCATG TCTGGGTAGG AGAAACTATC AACGGTGGTA CCCAGTTTGA ATATAATGTT 
GAAGGAGTAA CCACCAAGGA AATGGCTACC CAACTTGCCT TCATGCGTCT GCTGGCCAAC 
CATGCCTCTC AGAACATCAC CTACCATTGC AAGAACAGCA TTGCATACAT GGATGAGGAA 
ACTGGCAACC TGAAAAAGGC TGTCATTCTG CAAGGATCCA ATGATGTCGA ACTTGTTGCC 
GAGGGCAACA GCAGATTCAC TTACACTGTT CTTGTAGATG GCTGCTCTAA AAAGACAAAT 
GAATGGCAGA AGACAATCAT TGAATATAAA ACAAACAAGC CATCTCGCCT GCCTATCCTT 
GATATTGCAC CTTTGGACAT CGGTGGCGCT GACCAAGAAA TCAGATTGAA CATTGGCCCA 
GTCTGTTTCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Matrix-specific suppression of integrin activation in shear stress signaling.
Molecular biology of the cell17(11)4686-97(2006 Nov)
Orr AW,Ginsberg MH,Shattil SJ,Deckmyn H,Schwartz MA

Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells.
Journal of comparative pathology133(4)223-34(2005 Nov)
Wangoo A,Johnson L,Gough J,Ackbar R,Inglut S,Hicks D,Spencer Y,Hewinson G,Vordermeier M

The FAXWXXT motif in the carboxyl terminus of Vibrio mimicus metalloprotease is involved in binding to collagen.
FEBS letters579(11)2507-13(2005 Apr)
Lee JH,Ahn SH,Lee EM,Jeong SH,Kim YO,Lee SJ,Kong IS

Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors.
Biochemical and biophysical research communications307(4)948-55(2003 Aug)
Magklara A,Mellati AA,Wasney GA,Little SP,Sotiropoulou G,Becker GW,Diamandis EP

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COL1A2 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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