nedd4l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nedd4l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nedd4l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24906 NM_001006726.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26575 XM_012959670.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26576 XM_012959672.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26577 XM_012959676.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26578 XM_012959681.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26579 XM_012959686.1
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26580 XM_012959688.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26581 XM_012959692.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26582 XM_012959698.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26583 XM_012959709.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26584 XM_012959723.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26584 XM_012959720.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26584 XM_012959735.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26585 XM_012959732.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa47779 XM_012959735.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_031897927.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_031897930.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_012959720.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_012959723.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_031897928.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47779 XM_012959661.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47780 XM_012959732.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47780 XM_031897932.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47780 XM_012959663.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47781 XM_012959667.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47782 XM_012959670.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47783 XM_012959672.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47784 XM_012959676.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47785 XM_012959681.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47786 XM_012959688.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47787 XM_012959692.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47788 XM_012959698.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47789 XM_012959709.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47754 XM_012959594.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47755 XM_012959595.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47756 XM_012959597.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47757 XM_012959602.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47758 XM_012959606.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47759 XM_012959611.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47760 XM_012959617.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47761 XM_012959625.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47762 XM_012959632.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47763 XM_031897886.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47764 XM_012959635.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47765 XM_012959639.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47766 XM_012959640.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47767 XM_012959645.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47768 XM_012959649.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47769 XM_031897894.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47770 XM_012959652.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47771 XM_031897897.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47772 XM_012959653.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47773 XM_012959655.3
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa47774 XM_031897901.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47775 XM_031897902.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47776 XM_031897907.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47777 XM_031897913.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47778 XM_031897914.1
Latest version!
Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa26555 XM_012959594.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26556 XM_012959595.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26557 XM_012959597.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26558 XM_012959602.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26559 XM_012959606.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26560 XM_012959611.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26561 XM_012959617.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26562 XM_012959625.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26563 XM_012959632.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26564 XM_012959635.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26565 XM_012959639.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26566 XM_012959640.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26567 XM_012959645.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26568 XM_012959649.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26569 XM_012959652.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26570 XM_012959653.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26571 XM_012959655.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa26572 XM_012959661.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa26573 XM_012959663.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa26574 XM_012959667.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24906
    Clone ID Related Accession (Same CDS sequence) NM_001006726.1
    Accession Version NM_001006726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC075469.1. ##Evidence-Data-START## Transcript exon combination :: BC075469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001006727.1
    CDS142..3054
    Translation

    Target ORF information:

    RefSeq Version NM_001006726.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001006726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26575
    Clone ID Related Accession (Same CDS sequence) XM_012959670.2
    Accession Version XM_012959670.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA AACAGCTTGG GAGAGGCAAA AGCAGAGAGA ACCGTCCACG
    AGATTAAGGT CTTGCAGTGT AACAGATGGT GTCGCCGAGC AGGCTCATTT ATCACTGCCG
    AGCGTCTCAA CGGGAAGAGC TCGTTCCTCA ACTGTCACAG GCGGTGAGGA TTCTACGCCA
    ACCGTGGCCT ATGTGCACAC CACTCCGGGT TTGCCTTCAG GATGGGAGGA GAGGAAAGAC
    GCTAAAGGCC GTACCTATTA TGTCAATCAT AACAATCGCA CCACCACATG GACCAGACCT
    ATTGTGCAGC ACGCCGAGGA CGGGGCAGTG GGATCTACTT CAAACAGTAG TAACCATCTG
    AGCGAGCCTC AGATCCGACG GCCTCGTAGT CTCAGTTCTC CCACAGTTAC CCTATCTGCA
    CCACTCGAGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTCGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815124.1
    CDS575..3571
    Translation

    Target ORF information:

    RefSeq Version XM_012959670.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA AACAGCTTGG GAGAGGCAAA AGCAGAGAGA ACCGTCCACG
    AGATTAAGGT CTTGCAGTGT AACAGATGGT GTCGCCGAGC AGGCTCATTT ATCACTGCCG
    AGCGTCTCAA CGGGAAGAGC TCGTTCCTCA ACTGTCACAG GCGGTGAGGA TTCTACGCCA
    ACCGTGGCCT ATGTGCACAC CACTCCGGGT TTGCCTTCAG GATGGGAGGA GAGGAAAGAC
    GCTAAAGGCC GTACCTATTA TGTCAATCAT AACAATCGCA CCACCACATG GACCAGACCT
    ATTGTGCAGC ACGCCGAGGA CGGGGCAGTG GGATCTACTT CAAACAGTAG TAACCATCTG
    AGCGAGCCTC AGATCCGACG GCCTCGTAGT CTCAGTTCTC CCACAGTTAC CCTATCTGCA
    CCACTCGAGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTCGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26576
    Clone ID Related Accession (Same CDS sequence) XM_012959672.2
    Accession Version XM_012959672.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTTGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAAAC AGCTTGGGAG AGGCAAAAGC AGAGAGAACC GTCCACGAGA
    TTAAGGTCTT GCAGTGTAAC AGATGGTGTC GCCGAGCAGG CTCATTTATC ACTGCCGAGC
    GTCTCAACGG GAAGAGCTCG TTCCTCAACT GTCACAGGCG GTGAGGATTC TACGCCAACC
    GTGGCCTATG TGCACACCAC TCCGGGTTTG CCTTCAGGAT GGGAGGAGAG GAAAGACGCT
    AAAGGCCGTA CCTATTATGT CAATCATAAC AATCGCACCA CCACATGGAC CAGACCTATT
    GTGCAGCACG CCGAGGACGG GGCAGTGGGA TCTACTTCAA ACAGTAGTAA CCATCTGAGC
    GAGCCTCAGA TCCGACGGCC TCGTAGTCTC AGTTCTCCCA CAGTTACCCT ATCTGCACCA
    CTCGAGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTC
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815126.1
    CDS575..3568
    Translation

    Target ORF information:

    RefSeq Version XM_012959672.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTTGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAAAC AGCTTGGGAG AGGCAAAAGC AGAGAGAACC GTCCACGAGA
    TTAAGGTCTT GCAGTGTAAC AGATGGTGTC GCCGAGCAGG CTCATTTATC ACTGCCGAGC
    GTCTCAACGG GAAGAGCTCG TTCCTCAACT GTCACAGGCG GTGAGGATTC TACGCCAACC
    GTGGCCTATG TGCACACCAC TCCGGGTTTG CCTTCAGGAT GGGAGGAGAG GAAAGACGCT
    AAAGGCCGTA CCTATTATGT CAATCATAAC AATCGCACCA CCACATGGAC CAGACCTATT
    GTGCAGCACG CCGAGGACGG GGCAGTGGGA TCTACTTCAA ACAGTAGTAA CCATCTGAGC
    GAGCCTCAGA TCCGACGGCC TCGTAGTCTC AGTTCTCCCA CAGTTACCCT ATCTGCACCA
    CTCGAGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTC
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26577
    Clone ID Related Accession (Same CDS sequence) XM_012959676.2
    Accession Version XM_012959676.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTCGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815130.1
    CDS18..2975
    Translation

    Target ORF information:

    RefSeq Version XM_012959676.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959676.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTCGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26578
    Clone ID Related Accession (Same CDS sequence) XM_012959681.2
    Accession Version XM_012959681.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815135.1
    CDS18..2972
    Translation

    Target ORF information:

    RefSeq Version XM_012959681.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959681.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26579
    Clone ID Related Accession (Same CDS sequence) XM_012959686.1
    Accession Version XM_012959686.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815140.1
    CDS227..3142
    Translation

    Target ORF information:

    RefSeq Version XM_012959686.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959686.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTTG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26580
    Clone ID Related Accession (Same CDS sequence) XM_012959688.2
    Accession Version XM_012959688.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTCGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815142.1
    CDS17..2914
    Translation

    Target ORF information:

    RefSeq Version XM_012959688.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTCGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26581
    Clone ID Related Accession (Same CDS sequence) XM_012959692.2
    Accession Version XM_012959692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTTGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815146.1
    CDS160..3012
    Translation

    Target ORF information:

    RefSeq Version XM_012959692.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTTGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26582
    Clone ID Related Accession (Same CDS sequence) XM_012959698.2
    Accession Version XM_012959698.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGCAGGCCTG GGATGTGGTG
    GACTCCAATG ATTCAAGCTC TCCACATCAG CAAGAGCTCC CAGCTCCACC TATGCCGCCG
    GGATGGGAAG AAAAAGTGGA CAACCTTGGG CGGACATATT ACGTCAACCA TAATAACAAG
    ACCACTCAGT GGCAAAGGCC AAGCCTTGTA GACGTTACGT CCGAATCGGA CAGCAACATT
    CGGCACATAC AGCAAGAGGC CCACCGAGTG TTCCGATCTC GGAGACATAT CAGTGAAGAC
    CTAGAACCCG ACCATATAGA AGGAGGAGCG GATGTTCCAG AGCCATGGGA AACCATTTCC
    GAGGAAATGA CTTTGACGAC CGACACGTTA AACCAGTCTC TCCCACCCCC TGCTTCCCCG
    GATTCTCGGG CCAGCGCTCT GGAACTGTCC GAGGAGCTGA ACAGGAGGCT GCAGATTAGC
    TCCGAGTCCA ACGGAGAACA GTTCAGTTCT ATCATTCAAA CAGCTTGGGA GAGGCAAAAG
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815152.1
    CDS245..3019
    Translation

    Target ORF information:

    RefSeq Version XM_012959698.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959698.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGCAGGCCTG GGATGTGGTG
    GACTCCAATG ATTCAAGCTC TCCACATCAG CAAGAGCTCC CAGCTCCACC TATGCCGCCG
    GGATGGGAAG AAAAAGTGGA CAACCTTGGG CGGACATATT ACGTCAACCA TAATAACAAG
    ACCACTCAGT GGCAAAGGCC AAGCCTTGTA GACGTTACGT CCGAATCGGA CAGCAACATT
    CGGCACATAC AGCAAGAGGC CCACCGAGTG TTCCGATCTC GGAGACATAT CAGTGAAGAC
    CTAGAACCCG ACCATATAGA AGGAGGAGCG GATGTTCCAG AGCCATGGGA AACCATTTCC
    GAGGAAATGA CTTTGACGAC CGACACGTTA AACCAGTCTC TCCCACCCCC TGCTTCCCCG
    GATTCTCGGG CCAGCGCTCT GGAACTGTCC GAGGAGCTGA ACAGGAGGCT GCAGATTAGC
    TCCGAGTCCA ACGGAGAACA GTTCAGTTCT ATCATTCAAA CAGCTTGGGA GAGGCAAAAG
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26583
    Clone ID Related Accession (Same CDS sequence) XM_012959709.2
    Accession Version XM_012959709.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGGCCTGGGA TGTGGTGGAC
    TCCAATGATT CAAGCTCTCC ACATCAGCAA GAGCTCCCAG CTCCACCTAT GCCGCCGGGA
    TGGGAAGAAA AAGTGGACAA CCTTGGGCGG ACATATTACG TCAACCATAA TAACAAGACC
    ACTCAGTGGC AAAGGCCAAG CCTTGTAGAC GTTACGTCCG AATCGGACAG CAACATTCGG
    CACATACAGC AAGAGGCCCA CCGAGTGTTC CGATCTCGGA GACATATCAG TGAAGACCTA
    GAACCCGACC ATATAGAAGG AGGAGCGGAT GTTCCAGAGC CATGGGAAAC CATTTCCGAG
    GAAATGACTT TGACGACCGA CACGTTAAAC CAGTCTCTCC CACCCCCTGC TTCCCCGGAT
    TCTCGGGCCA GCGCTCTGGA ACTGTCCGAG GAGCTGAACA GGAGGCTGCA GATTAGCTCC
    GAGTCCAACG GAGAACAGTT CAGTTCTATC ATTCAAACAG CTTGGGAGAG GCAAAAGCAG
    AGAGAACCGT CCACGAGATT AAGGTCTTGC AGTGTAACAG ATGGTGTCGC CGAGCAGGCT
    CATTTATCAC TGCCGAGCGT CTCAACGGGA AGAGCTCGTT CCTCAACTGT CACAGGCGGT
    GAGGATTCTA CGCCAACCGT GGCCTATGTG CACACCACTC CGGGTTTGCC TTCAGGATGG
    GAGGAGAGGA AAGACGCTAA AGGCCGTACC TATTATGTCA ATCATAACAA TCGCACCACC
    ACATGGACCA GACCTATTGT GCAGCACGCC GAGGACGGGG CAGTGGGATC TACTTCAAAC
    AGTAGTAACC ATCTGAGCGA GCCTCAGATC CGACGGCCTC GTAGTCTCAG TTCTCCCACA
    GTTACCCTAT CTGCACCACT CGAGGGCGCA AAAGACTTCC CCGTGCGCAG AGCAGTGAAG
    GACACCCTGT CCAACCCACA GTCTCCACAG CCGTCACCTT ATAATTCTCC AAAGCCACAA
    CACAAAGGGG CCCAGAGCTT CTTGCCGCCG GGCTGGGAGA TGAGAATAGC ACCCAATGGC
    AGACCATTCT TTATTGACCA TAACACAAAA ACTACCACAT GGGAGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAGAAGT
    CCAGGGTTGA GGTGTCGAGA CACAGGTACC AGCTGCTCGG TGCCCCCCTA CGACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815163.1
    CDS246..3017
    Translation

    Target ORF information:

    RefSeq Version XM_012959709.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959709.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGGCCTGGGA TGTGGTGGAC
    TCCAATGATT CAAGCTCTCC ACATCAGCAA GAGCTCCCAG CTCCACCTAT GCCGCCGGGA
    TGGGAAGAAA AAGTGGACAA CCTTGGGCGG ACATATTACG TCAACCATAA TAACAAGACC
    ACTCAGTGGC AAAGGCCAAG CCTTGTAGAC GTTACGTCCG AATCGGACAG CAACATTCGG
    CACATACAGC AAGAGGCCCA CCGAGTGTTC CGATCTCGGA GACATATCAG TGAAGACCTA
    GAACCCGACC ATATAGAAGG AGGAGCGGAT GTTCCAGAGC CATGGGAAAC CATTTCCGAG
    GAAATGACTT TGACGACCGA CACGTTAAAC CAGTCTCTCC CACCCCCTGC TTCCCCGGAT
    TCTCGGGCCA GCGCTCTGGA ACTGTCCGAG GAGCTGAACA GGAGGCTGCA GATTAGCTCC
    GAGTCCAACG GAGAACAGTT CAGTTCTATC ATTCAAACAG CTTGGGAGAG GCAAAAGCAG
    AGAGAACCGT CCACGAGATT AAGGTCTTGC AGTGTAACAG ATGGTGTCGC CGAGCAGGCT
    CATTTATCAC TGCCGAGCGT CTCAACGGGA AGAGCTCGTT CCTCAACTGT CACAGGCGGT
    GAGGATTCTA CGCCAACCGT GGCCTATGTG CACACCACTC CGGGTTTGCC TTCAGGATGG
    GAGGAGAGGA AAGACGCTAA AGGCCGTACC TATTATGTCA ATCATAACAA TCGCACCACC
    ACATGGACCA GACCTATTGT GCAGCACGCC GAGGACGGGG CAGTGGGATC TACTTCAAAC
    AGTAGTAACC ATCTGAGCGA GCCTCAGATC CGACGGCCTC GTAGTCTCAG TTCTCCCACA
    GTTACCCTAT CTGCACCACT CGAGGGCGCA AAAGACTTCC CCGTGCGCAG AGCAGTGAAG
    GACACCCTGT CCAACCCACA GTCTCCACAG CCGTCACCTT ATAATTCTCC AAAGCCACAA
    CACAAAGGGG CCCAGAGCTT CTTGCCGCCG GGCTGGGAGA TGAGAATAGC ACCCAATGGC
    AGACCATTCT TTATTGACCA TAACACAAAA ACTACCACAT GGGAGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAGAAGT
    CCAGGGTTGA GGTGTCGAGA CACAGGTACC AGCTGCTCGG TGCCCCCCTA CGACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26584
    Clone ID Related Accession (Same CDS sequence) XM_012959735.2 , XM_012959723.2 , XM_012959720.2
    Accession Version XM_012959735.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815189.1
    CDS394..3036
    Translation

    Target ORF information:

    RefSeq Version XM_012959735.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959735.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26584
    Clone ID Related Accession (Same CDS sequence) XM_012959735.2 , XM_012959723.2 , XM_012959720.2
    Accession Version XM_012959723.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815177.1
    CDS194..2836
    Translation

    Target ORF information:

    RefSeq Version XM_012959723.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959723.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26584
    Clone ID Related Accession (Same CDS sequence) XM_012959735.2 , XM_012959723.2 , XM_012959720.2
    Accession Version XM_012959720.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815174.1
    CDS253..2895
    Translation

    Target ORF information:

    RefSeq Version XM_012959720.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959720.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTTGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTA
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTCG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26585
    Clone ID Related Accession (Same CDS sequence) XM_012959732.2
    Accession Version XM_012959732.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TTGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTATCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTCGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815186.1
    CDS175..2814
    Translation

    Target ORF information:

    RefSeq Version XM_012959732.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TTGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTATCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTCGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_012959735.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959735.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815189.1
    CDS384..3026
    Translation

    Target ORF information:

    RefSeq Version XM_012959735.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959735.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_031897927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753787.1
    CDS91..2733
    Translation

    Target ORF information:

    RefSeq Version XM_031897927.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_031897928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753788.1
    CDS91..2733
    Translation

    Target ORF information:

    RefSeq Version XM_031897928.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_031897930.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753790.1
    CDS220..2862
    Translation

    Target ORF information:

    RefSeq Version XM_031897930.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_012959723.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815177.1
    CDS166..2808
    Translation

    Target ORF information:

    RefSeq Version XM_012959723.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959723.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_012959720.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815174.1
    CDS242..2884
    Translation

    Target ORF information:

    RefSeq Version XM_012959720.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47779
    Clone ID Related Accession (Same CDS sequence) XM_012959735.3 , XM_031897927.1 , XM_031897928.1 , XM_031897930.1 , XM_012959723.3 , XM_012959720.3 , XM_012959661.3
    Accession Version XM_012959661.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815115.2
    CDS335..2977
    Translation

    Target ORF information:

    RefSeq Version XM_012959661.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959661.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAAACA GCTTGGGAGA GGCAAAAGCA GAGAGAACCG
    TCCACGAGAT TAAGGTCTTG CAGTGTAACA GATGGTGTCG CCGAGCAGGC TCATTTATCA
    CTGCCGAGCG TCTCAACGGG AAGAGCTCGT TCCTCAACTG TCACAGGCGG TGAGGATTCT
    ACGCCAACCG TGGCCTATGT GCACACCACT CCGGGTTTGC CTTCAGGATG GGAGGAGAGG
    AAAGACGCTA AAGGCCGTAC CTATTATGTC AATCATAACA ATCGCACCAC CACATGGACC
    AGACCTATTG TGCAGCACGC CGAGGACGGG GCAGTGGGAT CTACTTCAAA CAGTAGTAAC
    CATCTGAGCG AGCCTCAGAT CCGACGGCCT CGTAGTCTCA GTTCTCCCAC AGTTACCCTG
    TCTGCACCAC TCGAGGGCGC AAAAGACTTC CCCGTGCGCA GAGCAGTGAA GGACACCCTG
    TCCAACCCAC AGTCTCCACA GCCGTCACCT TATAATTCTC CAAAGCCACA ACACAAAGGG
    GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG ATGAGAATAG CACCCAATGG CAGACCATTC
    TTTATTGACC ATAACACAAA AACTACCACA TGGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47780
    Clone ID Related Accession (Same CDS sequence) XM_012959732.3 , XM_012959663.3 , XM_031897932.1
    Accession Version XM_012959732.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815186.1
    CDS175..2814
    Translation

    Target ORF information:

    RefSeq Version XM_012959732.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959732.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47780
    Clone ID Related Accession (Same CDS sequence) XM_012959732.3 , XM_012959663.3 , XM_031897932.1
    Accession Version XM_012959663.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815117.2
    CDS335..2974
    Translation

    Target ORF information:

    RefSeq Version XM_012959663.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47780
    Clone ID Related Accession (Same CDS sequence) XM_012959732.3 , XM_012959663.3 , XM_031897932.1
    Accession Version XM_031897932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753792.1
    CDS220..2859
    Translation

    Target ORF information:

    RefSeq Version XM_031897932.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG GCCTGGGATG TGGTGGACTC CAATGATTCA
    AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT CCACCTATGC CGCCGGGATG GGAAGAAAAA
    GTGGACAACC TTGGGCGGAC ATATTACGTC AACCATAATA ACAAGACCAC TCAGTGGCAA
    AGGCCAAGCC TCGTAGACGT TACGTCCGAA TCGGACAGCA ACATTCGGCA CATACAGCAA
    GAGGCCCACC GAGTGTTCCG ATCTCGGAGA CATATCAGTG AAGACCTAGA ACCCGACCAT
    ATAGAAGGAG GAGCGGATGT TCCAGAGCCA TGGGAAACCA TTTCCGAGGA AATGACTTTG
    ACGACCGACA CGTTAAACCA GTCTCTCCCA CCCCCTGCTT CCCCGGATTC TCGGGCCAGC
    GCTCTGGAAC TGTCCGAGGA GCTGAACAGG AGGCTGCAGA TTAGCTCCGA GTCCAACGGA
    GAACAGTTCA GTTCTATCAT TCAAACAGCT TGGGAGAGGC AAAAGCAGAG AGAACCGTCC
    ACGAGATTAA GGTCTTGCAG TGTAACAGAT GGTGTCGCCG AGCAGGCTCA TTTATCACTG
    CCGAGCGTCT CAACGGGAAG AGCTCGTTCC TCAACTGTCA CAGGCGGTGA GGATTCTACG
    CCAACCGTGG CCTATGTGCA CACCACTCCG GGTTTGCCTT CAGGATGGGA GGAGAGGAAA
    GACGCTAAAG GCCGTACCTA TTATGTCAAT CATAACAATC GCACCACCAC ATGGACCAGA
    CCTATTGTGC AGCACGCCGA GGACGGGGCA GTGGGATCTA CTTCAAACAG TAGTAACCAT
    CTGAGCGAGC CTCAGATCCG ACGGCCTCGT AGTCTCAGTT CTCCCACAGT TACCCTGTCT
    GCACCACTCG AGGGCGCAAA AGACTTCCCC GTGCGCAGAG CAGTGAAGGA CACCCTGTCC
    AACCCACAGT CTCCACAGCC GTCACCTTAT AATTCTCCAA AGCCACAACA CAAAGGGGCC
    CAGAGCTTCT TGCCGCCGGG CTGGGAGATG AGAATAGCAC CCAATGGCAG ACCATTCTTT
    ATTGACCATA ACACAAAAAC TACCACATGG GAGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47781
    Clone ID Related Accession (Same CDS sequence) XM_012959667.3
    Accession Version XM_012959667.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815121.2
    CDS335..2953
    Translation

    Target ORF information:

    RefSeq Version XM_012959667.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47782
    Clone ID Related Accession (Same CDS sequence) XM_012959670.3
    Accession Version XM_012959670.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTCG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA AACAGCTTGG GAGAGGCAAA AGCAGAGAGA ACCGTCCACG
    AGATTAAGGT CTTGCAGTGT AACAGATGGT GTCGCCGAGC AGGCTCATTT ATCACTGCCG
    AGCGTCTCAA CGGGAAGAGC TCGTTCCTCA ACTGTCACAG GCGGTGAGGA TTCTACGCCA
    ACCGTGGCCT ATGTGCACAC CACTCCGGGT TTGCCTTCAG GATGGGAGGA GAGGAAAGAC
    GCTAAAGGCC GTACCTATTA TGTCAATCAT AACAATCGCA CCACCACATG GACCAGACCT
    ATTGTGCAGC ACGCCGAGGA CGGGGCAGTG GGATCTACTT CAAACAGTAG TAACCATCTG
    AGCGAGCCTC AGATCCGACG GCCTCGTAGT CTCAGTTCTC CCACAGTTAC CCTGTCTGCA
    CCACTCGAGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTTGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815124.1
    CDS575..3571
    Translation

    Target ORF information:

    RefSeq Version XM_012959670.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGCAGGCC TGGGATGTGG TGGACTCCAA TGATTCAAGC
    TCTCCACATC AGCAAGAGCT CCCAGCTCCA CCTATGCCGC CGGGATGGGA AGAAAAAGTG
    GACAACCTTG GGCGGACATA TTACGTCAAC CATAATAACA AGACCACTCA GTGGCAAAGG
    CCAAGCCTCG TAGACGTTAC GTCCGAATCG GACAGCAACA TTCGGCACAT ACAGCAAGAG
    GCCCACCGAG TGTTCCGATC TCGGAGACAT ATCAGTGAAG ACCTAGAACC CGACCATATA
    GAAGGAGGAG CGGATGTTCC AGAGCCATGG GAAACCATTT CCGAGGAAAT GACTTTGACG
    ACCGACACGT TAAACCAGTC TCTCCCACCC CCTGCTTCCC CGGATTCTCG GGCCAGCGCT
    CTGGAACTGT CCGAGGAGCT GAACAGGAGG CTGCAGATTA GCTCCGAGTC CAACGGAGAA
    CAGTTCAGTT CTATCATTCA AACAGCTTGG GAGAGGCAAA AGCAGAGAGA ACCGTCCACG
    AGATTAAGGT CTTGCAGTGT AACAGATGGT GTCGCCGAGC AGGCTCATTT ATCACTGCCG
    AGCGTCTCAA CGGGAAGAGC TCGTTCCTCA ACTGTCACAG GCGGTGAGGA TTCTACGCCA
    ACCGTGGCCT ATGTGCACAC CACTCCGGGT TTGCCTTCAG GATGGGAGGA GAGGAAAGAC
    GCTAAAGGCC GTACCTATTA TGTCAATCAT AACAATCGCA CCACCACATG GACCAGACCT
    ATTGTGCAGC ACGCCGAGGA CGGGGCAGTG GGATCTACTT CAAACAGTAG TAACCATCTG
    AGCGAGCCTC AGATCCGACG GCCTCGTAGT CTCAGTTCTC CCACAGTTAC CCTGTCTGCA
    CCACTCGAGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTTGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47783
    Clone ID Related Accession (Same CDS sequence) XM_012959672.3
    Accession Version XM_012959672.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTCGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAAAC AGCTTGGGAG AGGCAAAAGC AGAGAGAACC GTCCACGAGA
    TTAAGGTCTT GCAGTGTAAC AGATGGTGTC GCCGAGCAGG CTCATTTATC ACTGCCGAGC
    GTCTCAACGG GAAGAGCTCG TTCCTCAACT GTCACAGGCG GTGAGGATTC TACGCCAACC
    GTGGCCTATG TGCACACCAC TCCGGGTTTG CCTTCAGGAT GGGAGGAGAG GAAAGACGCT
    AAAGGCCGTA CCTATTATGT CAATCATAAC AATCGCACCA CCACATGGAC CAGACCTATT
    GTGCAGCACG CCGAGGACGG GGCAGTGGGA TCTACTTCAA ACAGTAGTAA CCATCTGAGC
    GAGCCTCAGA TCCGACGGCC TCGTAGTCTC AGTTCTCCCA CAGTTACCCT GTCTGCACCA
    CTCGAGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTT
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815126.1
    CDS575..3568
    Translation

    Target ORF information:

    RefSeq Version XM_012959672.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959672.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTCGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAAAC AGCTTGGGAG AGGCAAAAGC AGAGAGAACC GTCCACGAGA
    TTAAGGTCTT GCAGTGTAAC AGATGGTGTC GCCGAGCAGG CTCATTTATC ACTGCCGAGC
    GTCTCAACGG GAAGAGCTCG TTCCTCAACT GTCACAGGCG GTGAGGATTC TACGCCAACC
    GTGGCCTATG TGCACACCAC TCCGGGTTTG CCTTCAGGAT GGGAGGAGAG GAAAGACGCT
    AAAGGCCGTA CCTATTATGT CAATCATAAC AATCGCACCA CCACATGGAC CAGACCTATT
    GTGCAGCACG CCGAGGACGG GGCAGTGGGA TCTACTTCAA ACAGTAGTAA CCATCTGAGC
    GAGCCTCAGA TCCGACGGCC TCGTAGTCTC AGTTCTCCCA CAGTTACCCT GTCTGCACCA
    CTCGAGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTT
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47784
    Clone ID Related Accession (Same CDS sequence) XM_012959676.3
    Accession Version XM_012959676.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAACACCAAA ATAACCCAAT GGGAGGACCC CCGGCTTCAG
    AATCCCGCCA TCACTGGCCC TGCCGTCCCC TACTCAAGAG AGTTTAAGCA GAAATACGAC
    TACTTCAGGA AGAAGTTGAA GAAACCTGCG GATATTCCAA ATCGATTTGA GATGAAGCTT
    CACAGAAACA ACATTTTCGA GGAATCCTAT AGGAGGATAA TGTCGGTGAA GCGGCCGGAT
    GTCCTTAAAG CCAGATTATG GATTGAATTT GAGTCGGAAA AAGGCCTCGA TTATGGAGGA
    GTAGCAAGGG AATGGTTCTT CCTTCTGTCC AAAGAGATGT TCAACCCATA CTACGGGCTG
    TTTGAGTACT CCGCTACGGA CAATTACACA CTTCAGATAA ATCCCAATTC GGGTCTTTGC
    AATGAAGATC ACCTTTCGTA CTTCACGTTT ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC
    CATGGAAAAT TATTAGATGG TTTCTTCATT AGACCATTCT ATAAGATGAT GCTGGGGAAA
    CAAATAACGC TGAAAGACAT GGAGTCAGTG GACAGTGAAT ACTACAATTC CTTAAAATGG
    ATCTTAGAAA ACGACCCAAC CGAGCTGGAT CTCAGGTTTT GCATAGATGA AGAAAACTTC
    GGTCAGACGT ATCAGGTGGA TCTGAAGCCC AATGGATCTG AGATGGTCGT GACCAATGAC
    AACAAGCGAG AGTACATTGA CCTCGTGATC CAATGGAGGT TTGTCAACCG GGTACAGAAG
    CAGATGAATG CCTTTTTAGA AGGTTTTACT GAACTCATTC CTATTGATCT AATAAAAATC
    TTTGATGAAA ATGAGCTGGA GTTGCTAATG TGCGGCCTTG GTGATGTTGA TGTGAATGAC
    TGGAGGCAAC ACACCCTGTA TAAGAACGGC TACTGCCCCA ACCACCCCGC CATACAGTGG
    TTCTGGAAGG CGGTGTTATT AATGGATGCA GAGAAACGCA TACGCTTGCT GCAGTTTGTG
    ACTGGGACGT CACGTGTGCC TATGAATGGA TTTGCTGAAC TGTACGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA GTGGGGAAGC CCCGACAAGC TGCCCAGAGC TCACACATGC
    TTTAACCGCC TGGACTTACC TCCATACGAC TCGTTTGAGG ATTTACGGGA GAAGCTTCTC
    ATGGCCGTGG AGAACGCTCA GGGATTCGAA GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815130.2
    CDS335..2896
    Translation

    Target ORF information:

    RefSeq Version XM_012959676.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959676.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAACACCAAA ATAACCCAAT GGGAGGACCC CCGGCTTCAG
    AATCCCGCCA TCACTGGCCC TGCCGTCCCC TACTCAAGAG AGTTTAAGCA GAAATACGAC
    TACTTCAGGA AGAAGTTGAA GAAACCTGCG GATATTCCAA ATCGATTTGA GATGAAGCTT
    CACAGAAACA ACATTTTCGA GGAATCCTAT AGGAGGATAA TGTCGGTGAA GCGGCCGGAT
    GTCCTTAAAG CCAGATTATG GATTGAATTT GAGTCGGAAA AAGGCCTCGA TTATGGAGGA
    GTAGCAAGGG AATGGTTCTT CCTTCTGTCC AAAGAGATGT TCAACCCATA CTACGGGCTG
    TTTGAGTACT CCGCTACGGA CAATTACACA CTTCAGATAA ATCCCAATTC GGGTCTTTGC
    AATGAAGATC ACCTTTCGTA CTTCACGTTT ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC
    CATGGAAAAT TATTAGATGG TTTCTTCATT AGACCATTCT ATAAGATGAT GCTGGGGAAA
    CAAATAACGC TGAAAGACAT GGAGTCAGTG GACAGTGAAT ACTACAATTC CTTAAAATGG
    ATCTTAGAAA ACGACCCAAC CGAGCTGGAT CTCAGGTTTT GCATAGATGA AGAAAACTTC
    GGTCAGACGT ATCAGGTGGA TCTGAAGCCC AATGGATCTG AGATGGTCGT GACCAATGAC
    AACAAGCGAG AGTACATTGA CCTCGTGATC CAATGGAGGT TTGTCAACCG GGTACAGAAG
    CAGATGAATG CCTTTTTAGA AGGTTTTACT GAACTCATTC CTATTGATCT AATAAAAATC
    TTTGATGAAA ATGAGCTGGA GTTGCTAATG TGCGGCCTTG GTGATGTTGA TGTGAATGAC
    TGGAGGCAAC ACACCCTGTA TAAGAACGGC TACTGCCCCA ACCACCCCGC CATACAGTGG
    TTCTGGAAGG CGGTGTTATT AATGGATGCA GAGAAACGCA TACGCTTGCT GCAGTTTGTG
    ACTGGGACGT CACGTGTGCC TATGAATGGA TTTGCTGAAC TGTACGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA GTGGGGAAGC CCCGACAAGC TGCCCAGAGC TCACACATGC
    TTTAACCGCC TGGACTTACC TCCATACGAC TCGTTTGAGG ATTTACGGGA GAAGCTTCTC
    ATGGCCGTGG AGAACGCTCA GGGATTCGAA GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47785
    Clone ID Related Accession (Same CDS sequence) XM_012959681.3
    Accession Version XM_012959681.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959681.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815135.2
    CDS335..2893
    Translation

    Target ORF information:

    RefSeq Version XM_012959681.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959681.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47786
    Clone ID Related Accession (Same CDS sequence) XM_012959688.3
    Accession Version XM_012959688.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAACACCAAA ATAACCCAAT GGGAGGACCC CCGGCTTCAG
    AATCCCGCCA TCACTGGCCC TGCCGTCCCC TACTCAAGAG AGTTTAAGCA GAAATACGAC
    TACTTCAGGA AGAAGTTGAA GAAACCTGCG GATATTCCAA ATCGATTTGA GATGAAGCTT
    CACAGAAACA ACATTTTCGA GGAATCCTAT AGGAGGATAA TGTCGGTGAA GCGGCCGGAT
    GTCCTTAAAG CCAGATTATG GATTGAATTT GAGTCGGAAA AAGGCCTCGA TTATGGAGGA
    GTAGCAAGGG AATGGTTCTT CCTTCTGTCC AAAGAGATGT TCAACCCATA CTACGGGCTG
    TTTGAGTACT CCGCTACGGA CAATTACACA CTTCAGATAA ATCCCAATTC GGGTCTTTGC
    AATGAAGATC ACCTTTCGTA CTTCACGTTT ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC
    CATGGAAAAT TATTAGATGG TTTCTTCATT AGACCATTCT ATAAGATGAT GCTGGGGAAA
    CAAATAACGC TGAAAGACAT GGAGTCAGTG GACAGTGAAT ACTACAATTC CTTAAAATGG
    ATCTTAGAAA ACGACCCAAC CGAGCTGGAT CTCAGGTTTT GCATAGATGA AGAAAACTTC
    GGTCAGACGT ATCAGGTGGA TCTGAAGCCC AATGGATCTG AGATGGTCGT GACCAATGAC
    AACAAGCGAG AGTACATTGA CCTCGTGATC CAATGGAGGT TTGTCAACCG GGTACAGAAG
    CAGATGAATG CCTTTTTAGA AGGTTTTACT GAACTCATTC CTATTGATCT AATAAAAATC
    TTTGATGAAA ATGAGCTGGA GTTGCTAATG TGCGGCCTTG GTGATGTTGA TGTGAATGAC
    TGGAGGCAAC ACACCCTGTA TAAGAACGGC TACTGCCCCA ACCACCCCGC CATACAGTGG
    TTCTGGAAGG CGGTGTTATT AATGGATGCA GAGAAACGCA TACGCTTGCT GCAGTTTGTG
    ACTGGGACGT CACGTGTGCC TATGAATGGA TTTGCTGAAC TGTACGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA GTGGGGAAGC CCCGACAAGC TGCCCAGAGC TCACACATGC
    TTTAACCGCC TGGACTTACC TCCATACGAC TCGTTTGAGG ATTTACGGGA GAAGCTTCTC
    ATGGCCGTGG AGAACGCTCA GGGATTCGAA GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815142.2
    CDS335..2836
    Translation

    Target ORF information:

    RefSeq Version XM_012959688.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959688.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAAGGC CATACACATT CAAAGACTTC TTACTCAGAC CAAGAAGCCA CAAATCCCGG 
    GTTAAGGGTT TCTTGAGACT GAAGATGGCC TACTTGCCAA AGAATGGAAT GCAGGAAGAG
    GAGAATAATG AGCAAAGAGA AGAATCCGAG CAGGCCTGGG ATGTGGTGGA CTCCAATGAT
    TCAAGCTCTC CACATCAGCA AGAGCTCCCA GCTCCACCTA TGCCGCCGGG ATGGGAAGAA
    AAAGTGGACA ACCTTGGGCG GACATATTAC GTCAACCATA ATAACAAGAC CACTCAGTGG
    CAAAGGCCAA GCCTCGTAGA CGTTACGTCC GAATCGGACA GCAACATTCG GCACATACAG
    CAAGAGGCCC ACCGAGTGTT CCGATCTCGG AGACATATCA GTGAAGACCT AGAACCCGAC
    CATATAGAAG GAGGAGCGGA TGTTCCAGAG CCATGGGAAA CCATTTCCGA GGAAATGACT
    TTGACGACCG ACACGTTAAA CCAGTCTCTC CCACCCCCTG CTTCCCCGGA TTCTCGGGCC
    AGCGCTCTGG AACTGTCCGA GGAGCTGAAC AGGAGGCTGC AGATTAGCTC CGAGTCCAAC
    GGAGAACAGT TCAGTTCTAT CATTCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTGTCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAACACCAAA ATAACCCAAT GGGAGGACCC CCGGCTTCAG
    AATCCCGCCA TCACTGGCCC TGCCGTCCCC TACTCAAGAG AGTTTAAGCA GAAATACGAC
    TACTTCAGGA AGAAGTTGAA GAAACCTGCG GATATTCCAA ATCGATTTGA GATGAAGCTT
    CACAGAAACA ACATTTTCGA GGAATCCTAT AGGAGGATAA TGTCGGTGAA GCGGCCGGAT
    GTCCTTAAAG CCAGATTATG GATTGAATTT GAGTCGGAAA AAGGCCTCGA TTATGGAGGA
    GTAGCAAGGG AATGGTTCTT CCTTCTGTCC AAAGAGATGT TCAACCCATA CTACGGGCTG
    TTTGAGTACT CCGCTACGGA CAATTACACA CTTCAGATAA ATCCCAATTC GGGTCTTTGC
    AATGAAGATC ACCTTTCGTA CTTCACGTTT ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC
    CATGGAAAAT TATTAGATGG TTTCTTCATT AGACCATTCT ATAAGATGAT GCTGGGGAAA
    CAAATAACGC TGAAAGACAT GGAGTCAGTG GACAGTGAAT ACTACAATTC CTTAAAATGG
    ATCTTAGAAA ACGACCCAAC CGAGCTGGAT CTCAGGTTTT GCATAGATGA AGAAAACTTC
    GGTCAGACGT ATCAGGTGGA TCTGAAGCCC AATGGATCTG AGATGGTCGT GACCAATGAC
    AACAAGCGAG AGTACATTGA CCTCGTGATC CAATGGAGGT TTGTCAACCG GGTACAGAAG
    CAGATGAATG CCTTTTTAGA AGGTTTTACT GAACTCATTC CTATTGATCT AATAAAAATC
    TTTGATGAAA ATGAGCTGGA GTTGCTAATG TGCGGCCTTG GTGATGTTGA TGTGAATGAC
    TGGAGGCAAC ACACCCTGTA TAAGAACGGC TACTGCCCCA ACCACCCCGC CATACAGTGG
    TTCTGGAAGG CGGTGTTATT AATGGATGCA GAGAAACGCA TACGCTTGCT GCAGTTTGTG
    ACTGGGACGT CACGTGTGCC TATGAATGGA TTTGCTGAAC TGTACGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA GTGGGGAAGC CCCGACAAGC TGCCCAGAGC TCACACATGC
    TTTAACCGCC TGGACTTACC TCCATACGAC TCGTTTGAGG ATTTACGGGA GAAGCTTCTC
    ATGGCCGTGG AGAACGCTCA GGGATTCGAA GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47787
    Clone ID Related Accession (Same CDS sequence) XM_012959692.3
    Accession Version XM_012959692.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTCGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815146.1
    CDS160..3012
    Translation

    Target ORF information:

    RefSeq Version XM_012959692.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959692.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCTG AACCCATTTA TGGACTCTCA GAAGACGATG AGGAGTCGCG CATCCTCAGA 
    GTAAAAATTG TTTCCGGCAC TGACTTGGCA AAGAAGGATA TATTTGGGGC CAGTGATCCC
    TATGTGAAAC TGTCCCTGTA TGTGGCGGAT GAAAACCGTG AGCTGGCTCT GGTACAGACA
    AAGACAATAA AGAAGACCTT GAACCCAAAA TGGAATGAAG AATTTTTTTT CAGGGTTCAC
    CCGACCAATC ACAGGCTGCT GTTTGAAGTA TTTGATGAGA ACCGATTGAC AAGAGATGAT
    TTTTTGGGCC AAGTTGATGT TCCGCTGAAT CACTTACCGA CAGAAGATCC AACAATGGAA
    AGGCCATACA CATTCAAAGA CTTCTTACTC AGACCAAGAA GCCACAAATC CCGGGTTAAG
    GGTTTCTTGA GACTGAAGAT GGCCTACTTG CCAAAGAATG GAATGCAGGA AGAGGAGAAT
    AATGAGCAAA GAGAAGAATC CGAGGCCTGG GATGTGGTGG ACTCCAATGA TTCAAGCTCT
    CCACATCAGC AAGAGCTCCC AGCTCCACCT ATGCCGCCGG GATGGGAAGA AAAAGTGGAC
    AACCTTGGGC GGACATATTA CGTCAACCAT AATAACAAGA CCACTCAGTG GCAAAGGCCA
    AGCCTCGTAG ACGTTACGTC CGAATCGGAC AGCAACATTC GGCACATACA GCAAGAGGCC
    CACCGAGTGT TCCGATCTCG GAGACATATC AGTGAAGACC TAGAACCCGA CCATATAGAA
    GGAGGAGCGG ATGTTCCAGA GCCATGGGAA ACCATTTCCG AGGAAATGAC TTTGACGACC
    GACACGTTAA ACCAGTCTCT CCCACCCCCT GCTTCCCCGG ATTCTCGGGC CAGCGCTCTG
    GAACTGTCCG AGGAGCTGAA CAGGAGGCTG CAGATTAGCT CCGAGTCCAA CGGAGAACAG
    TTCAGTTCTA TCATTCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47788
    Clone ID Related Accession (Same CDS sequence) XM_012959698.3
    Accession Version XM_012959698.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGCAGGCCTG GGATGTGGTG
    GACTCCAATG ATTCAAGCTC TCCACATCAG CAAGAGCTCC CAGCTCCACC TATGCCGCCG
    GGATGGGAAG AAAAAGTGGA CAACCTTGGG CGGACATATT ACGTCAACCA TAATAACAAG
    ACCACTCAGT GGCAAAGGCC AAGCCTCGTA GACGTTACGT CCGAATCGGA CAGCAACATT
    CGGCACATAC AGCAAGAGGC CCACCGAGTG TTCCGATCTC GGAGACATAT CAGTGAAGAC
    CTAGAACCCG ACCATATAGA AGGAGGAGCG GATGTTCCAG AGCCATGGGA AACCATTTCC
    GAGGAAATGA CTTTGACGAC CGACACGTTA AACCAGTCTC TCCCACCCCC TGCTTCCCCG
    GATTCTCGGG CCAGCGCTCT GGAACTGTCC GAGGAGCTGA ACAGGAGGCT GCAGATTAGC
    TCCGAGTCCA ACGGAGAACA GTTCAGTTCT ATCATTCAAA CAGCTTGGGA GAGGCAAAAG
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TGTCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT TGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815152.1
    CDS237..3011
    Translation

    Target ORF information:

    RefSeq Version XM_012959698.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959698.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGCAGGCCTG GGATGTGGTG
    GACTCCAATG ATTCAAGCTC TCCACATCAG CAAGAGCTCC CAGCTCCACC TATGCCGCCG
    GGATGGGAAG AAAAAGTGGA CAACCTTGGG CGGACATATT ACGTCAACCA TAATAACAAG
    ACCACTCAGT GGCAAAGGCC AAGCCTCGTA GACGTTACGT CCGAATCGGA CAGCAACATT
    CGGCACATAC AGCAAGAGGC CCACCGAGTG TTCCGATCTC GGAGACATAT CAGTGAAGAC
    CTAGAACCCG ACCATATAGA AGGAGGAGCG GATGTTCCAG AGCCATGGGA AACCATTTCC
    GAGGAAATGA CTTTGACGAC CGACACGTTA AACCAGTCTC TCCCACCCCC TGCTTCCCCG
    GATTCTCGGG CCAGCGCTCT GGAACTGTCC GAGGAGCTGA ACAGGAGGCT GCAGATTAGC
    TCCGAGTCCA ACGGAGAACA GTTCAGTTCT ATCATTCAAA CAGCTTGGGA GAGGCAAAAG
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TGTCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT TGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47789
    Clone ID Related Accession (Same CDS sequence) XM_012959709.3
    Accession Version XM_012959709.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959709.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGGCCTGGGA TGTGGTGGAC
    TCCAATGATT CAAGCTCTCC ACATCAGCAA GAGCTCCCAG CTCCACCTAT GCCGCCGGGA
    TGGGAAGAAA AAGTGGACAA CCTTGGGCGG ACATATTACG TCAACCATAA TAACAAGACC
    ACTCAGTGGC AAAGGCCAAG CCTCGTAGAC GTTACGTCCG AATCGGACAG CAACATTCGG
    CACATACAGC AAGAGGCCCA CCGAGTGTTC CGATCTCGGA GACATATCAG TGAAGACCTA
    GAACCCGACC ATATAGAAGG AGGAGCGGAT GTTCCAGAGC CATGGGAAAC CATTTCCGAG
    GAAATGACTT TGACGACCGA CACGTTAAAC CAGTCTCTCC CACCCCCTGC TTCCCCGGAT
    TCTCGGGCCA GCGCTCTGGA ACTGTCCGAG GAGCTGAACA GGAGGCTGCA GATTAGCTCC
    GAGTCCAACG GAGAACAGTT CAGTTCTATC ATTCAAACAG CTTGGGAGAG GCAAAAGCAG
    AGAGAACCGT CCACGAGATT AAGGTCTTGC AGTGTAACAG ATGGTGTCGC CGAGCAGGCT
    CATTTATCAC TGCCGAGCGT CTCAACGGGA AGAGCTCGTT CCTCAACTGT CACAGGCGGT
    GAGGATTCTA CGCCAACCGT GGCCTATGTG CACACCACTC CGGGTTTGCC TTCAGGATGG
    GAGGAGAGGA AAGACGCTAA AGGCCGTACC TATTATGTCA ATCATAACAA TCGCACCACC
    ACATGGACCA GACCTATTGT GCAGCACGCC GAGGACGGGG CAGTGGGATC TACTTCAAAC
    AGTAGTAACC ATCTGAGCGA GCCTCAGATC CGACGGCCTC GTAGTCTCAG TTCTCCCACA
    GTTACCCTGT CTGCACCACT CGAGGGCGCA AAAGACTTCC CCGTGCGCAG AGCAGTGAAG
    GACACCCTGT CCAACCCACA GTCTCCACAG CCGTCACCTT ATAATTCTCC AAAGCCACAA
    CACAAAGGGG CCCAGAGCTT CTTGCCGCCG GGCTGGGAGA TGAGAATAGC ACCCAATGGC
    AGACCATTCT TTATTGACCA TAACACAAAA ACTACCACAT GGGAGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAGAAGT
    CCAGGGTTGA GGTGTCGAGA CACAGGTACC AGCTGCTCGG TGCCCCCCTA CGACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815163.1
    CDS202..2973
    Translation

    Target ORF information:

    RefSeq Version XM_012959709.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959709.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTACCGGG CTAGAGGCGA CCGATACAGC GCTCTGCTAA ATGATGCCCA AGGTCGAGAT 
    GGACAGACAA GAGATGATTT TTTGGGCCAA GTTGATGTTC CGCTGAATCA CTTACCGACA
    GAAGATCCAA CAATGGAAAG GCCATACACA TTCAAAGACT TCTTACTCAG ACCAAGAAGC
    CACAAATCCC GGGTTAAGGG TTTCTTGAGA CTGAAGATGG CCTACTTGCC AAAGAATGGA
    ATGCAGGAAG AGGAGAATAA TGAGCAAAGA GAAGAATCCG AGGCCTGGGA TGTGGTGGAC
    TCCAATGATT CAAGCTCTCC ACATCAGCAA GAGCTCCCAG CTCCACCTAT GCCGCCGGGA
    TGGGAAGAAA AAGTGGACAA CCTTGGGCGG ACATATTACG TCAACCATAA TAACAAGACC
    ACTCAGTGGC AAAGGCCAAG CCTCGTAGAC GTTACGTCCG AATCGGACAG CAACATTCGG
    CACATACAGC AAGAGGCCCA CCGAGTGTTC CGATCTCGGA GACATATCAG TGAAGACCTA
    GAACCCGACC ATATAGAAGG AGGAGCGGAT GTTCCAGAGC CATGGGAAAC CATTTCCGAG
    GAAATGACTT TGACGACCGA CACGTTAAAC CAGTCTCTCC CACCCCCTGC TTCCCCGGAT
    TCTCGGGCCA GCGCTCTGGA ACTGTCCGAG GAGCTGAACA GGAGGCTGCA GATTAGCTCC
    GAGTCCAACG GAGAACAGTT CAGTTCTATC ATTCAAACAG CTTGGGAGAG GCAAAAGCAG
    AGAGAACCGT CCACGAGATT AAGGTCTTGC AGTGTAACAG ATGGTGTCGC CGAGCAGGCT
    CATTTATCAC TGCCGAGCGT CTCAACGGGA AGAGCTCGTT CCTCAACTGT CACAGGCGGT
    GAGGATTCTA CGCCAACCGT GGCCTATGTG CACACCACTC CGGGTTTGCC TTCAGGATGG
    GAGGAGAGGA AAGACGCTAA AGGCCGTACC TATTATGTCA ATCATAACAA TCGCACCACC
    ACATGGACCA GACCTATTGT GCAGCACGCC GAGGACGGGG CAGTGGGATC TACTTCAAAC
    AGTAGTAACC ATCTGAGCGA GCCTCAGATC CGACGGCCTC GTAGTCTCAG TTCTCCCACA
    GTTACCCTGT CTGCACCACT CGAGGGCGCA AAAGACTTCC CCGTGCGCAG AGCAGTGAAG
    GACACCCTGT CCAACCCACA GTCTCCACAG CCGTCACCTT ATAATTCTCC AAAGCCACAA
    CACAAAGGGG CCCAGAGCTT CTTGCCGCCG GGCTGGGAGA TGAGAATAGC ACCCAATGGC
    AGACCATTCT TTATTGACCA TAACACAAAA ACTACCACAT GGGAGGATCC GCGACTGAAG
    TTCCCAGTAC ACATGAGAAC AAAAGCTTCC TTGAACCCTA ATGATCTGGG TCCTCTCCCT
    CCGGGCTGGG AGGAAAGAAT CCACGGGGAT GGGCGAACGT TCTATATCGA TCACAGAAGT
    CCAGGGTTGA GGTGTCGAGA CACAGGTACC AGCTGCTCGG TGCCCCCCTA CGACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47754
    Clone ID Related Accession (Same CDS sequence) XM_012959594.3
    Accession Version XM_012959594.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815048.1
    CDS609..4481
    Translation

    Target ORF information:

    RefSeq Version XM_012959594.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959594.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47755
    Clone ID Related Accession (Same CDS sequence) XM_012959595.3
    Accession Version XM_012959595.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815049.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959595.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959595.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47756
    Clone ID Related Accession (Same CDS sequence) XM_012959597.3
    Accession Version XM_012959597.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    GCCTGGGATG TGGTGGACTC CAATGATTCA AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT
    CCACCTATGC CGCCGGGATG GGAAGAAAAA GTGGACAACC TTGGGCGGAC ATATTACGTC
    AACCATAATA ACAAGACCAC TCAGTGGCAA AGGCCAAGCC TCGTAGACGT TACGTCCGAA
    TCGGACAGCA ACATTCGGCA CATACAGCAA GAGGCCCACC GAGTGTTCCG ATCTCGGAGA
    CATATCAGTG AAGACCTAGA ACCCGACCAT ATAGAAGGAG GAGCGGATGT TCCAGAGCCA
    TGGGAAACCA TTTCCGAGGA AATGACTTTG ACGACCGACA CGTTAAACCA GTCTCTCCCA
    CCCCCTGCTT CCCCGGATTC TCGGGCCAGC GCTCTGGAAC TGTCCGAGGA GCTGAACAGG
    AGGCTGCAGA TTAGCTCCGA GTCCAACGGA GAACAGTTCA GTTCTATCAT TCAAACAGCT
    TGGGAGAGGC AAAAGCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815051.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959597.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959597.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    GCCTGGGATG TGGTGGACTC CAATGATTCA AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT
    CCACCTATGC CGCCGGGATG GGAAGAAAAA GTGGACAACC TTGGGCGGAC ATATTACGTC
    AACCATAATA ACAAGACCAC TCAGTGGCAA AGGCCAAGCC TCGTAGACGT TACGTCCGAA
    TCGGACAGCA ACATTCGGCA CATACAGCAA GAGGCCCACC GAGTGTTCCG ATCTCGGAGA
    CATATCAGTG AAGACCTAGA ACCCGACCAT ATAGAAGGAG GAGCGGATGT TCCAGAGCCA
    TGGGAAACCA TTTCCGAGGA AATGACTTTG ACGACCGACA CGTTAAACCA GTCTCTCCCA
    CCCCCTGCTT CCCCGGATTC TCGGGCCAGC GCTCTGGAAC TGTCCGAGGA GCTGAACAGG
    AGGCTGCAGA TTAGCTCCGA GTCCAACGGA GAACAGTTCA GTTCTATCAT TCAAACAGCT
    TGGGAGAGGC AAAAGCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47757
    Clone ID Related Accession (Same CDS sequence) XM_012959602.3
    Accession Version XM_012959602.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959602.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815056.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959602.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959602.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47758
    Clone ID Related Accession (Same CDS sequence) XM_012959606.3
    Accession Version XM_012959606.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815060.1
    CDS609..4457
    Translation

    Target ORF information:

    RefSeq Version XM_012959606.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959606.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47759
    Clone ID Related Accession (Same CDS sequence) XM_012959611.3
    Accession Version XM_012959611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TTGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815065.1
    CDS609..4454
    Translation

    Target ORF information:

    RefSeq Version XM_012959611.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TTGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47760
    Clone ID Related Accession (Same CDS sequence) XM_012959617.3
    Accession Version XM_012959617.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TTGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815071.1
    CDS609..4424
    Translation

    Target ORF information:

    RefSeq Version XM_012959617.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959617.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TTGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47761
    Clone ID Related Accession (Same CDS sequence) XM_012959625.3
    Accession Version XM_012959625.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815079.1
    CDS609..4421
    Translation

    Target ORF information:

    RefSeq Version XM_012959625.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959625.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47762
    Clone ID Related Accession (Same CDS sequence) XM_012959632.3
    Accession Version XM_012959632.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815086.1
    CDS609..4421
    Translation

    Target ORF information:

    RefSeq Version XM_012959632.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959632.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47763
    Clone ID Related Accession (Same CDS sequence) XM_031897886.1
    Accession Version XM_031897886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753746.1
    CDS609..4418
    Translation

    Target ORF information:

    RefSeq Version XM_031897886.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTGTCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTTGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47764
    Clone ID Related Accession (Same CDS sequence) XM_012959635.3
    Accession Version XM_012959635.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815089.1
    CDS609..4400
    Translation

    Target ORF information:

    RefSeq Version XM_012959635.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959635.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47765
    Clone ID Related Accession (Same CDS sequence) XM_012959639.3
    Accession Version XM_012959639.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815093.1
    CDS609..4397
    Translation

    Target ORF information:

    RefSeq Version XM_012959639.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959639.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47766
    Clone ID Related Accession (Same CDS sequence) XM_012959640.3
    Accession Version XM_012959640.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815094.1
    CDS609..4397
    Translation

    Target ORF information:

    RefSeq Version XM_012959640.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959640.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTTGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47767
    Clone ID Related Accession (Same CDS sequence) XM_012959645.3
    Accession Version XM_012959645.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TTGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815099.1
    CDS609..4394
    Translation

    Target ORF information:

    RefSeq Version XM_012959645.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959645.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTGTCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TTGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47768
    Clone ID Related Accession (Same CDS sequence) XM_012959649.3
    Accession Version XM_012959649.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815103.1
    CDS609..4340
    Translation

    Target ORF information:

    RefSeq Version XM_012959649.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959649.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTGTCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTTGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47769
    Clone ID Related Accession (Same CDS sequence) XM_031897894.1
    Accession Version XM_031897894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT TGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753754.1
    CDS609..4283
    Translation

    Target ORF information:

    RefSeq Version XM_031897894.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTG TCTGCACCAC TCGAGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT TGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47770
    Clone ID Related Accession (Same CDS sequence) XM_012959652.3
    Accession Version XM_012959652.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGGCGCAA AAGACTTCCC CGTGCGCAGA
    GCAGTGAAGG ACACCCTGTC CAACCCACAG TCTCCACAGC CGTCACCTTA TAATTCTCCA
    AAGCCACAAC ACAAAGGGGC CCAGAGCTTC TTGCCGCCGG GCTGGGAGAT GAGAATAGCA
    CCCAATGGCA GACCATTCTT TATTGACCAT AACACAAAAA CTACCACATG GGAGGATCCG
    CGACTGAAGT TCCCAGTACA CATGAGAACA AAAGCTTCCT TGAACCCTAA TGATCTGGGT
    CCTCTCCCTC CGGGCTGGGA GGAAAGAATC CACGGGGATG GGCGAACGTT CTATATCGAT
    CACAGAAGTC CAGGGTTGAG GTGTCGAGAC ACAGGTACCA GCTGCTCGGT GCCCCCCTAC
    GACACCAAAA TAACCCAATG GGAGGACCCC CGGCTTCAGA ATCCCGCCAT CACTGGCCCT
    GCCGTCCCCT ACTCAAGAGA GTTTAAGCAG AAATACGACT ACTTCAGGAA GAAGTTGAAG
    AAACCTGCGG ATATTCCAAA TCGATTTGAG ATGAAGCTTC ACAGAAACAA CATTTTCGAG
    GAATCCTATA GGAGGATAAT GTCGGTGAAG CGGCCGGATG TCCTTAAAGC CAGATTATGG
    ATTGAATTTG AGTCGGAAAA AGGCCTCGAT TATGGAGGAG TAGCAAGGGA ATGGTTCTTC
    CTTCTGTCCA AAGAGATGTT CAACCCATAC TACGGGCTGT TTGAGTACTC CGCTACGGAC
    AATTACACAC TTCAGATAAA TCCCAATTCG GGTCTTTGCA ATGAAGATCA CCTTTCGTAC
    TTCACGTTTA TTGGTCGGAT TGCTGGGCTC GCCGTGTTCC ATGGAAAATT ATTAGATGGT
    TTCTTCATTA GACCATTCTA TAAGATGATG CTGGGGAAAC AAATAACGCT GAAAGACATG
    GAGTCAGTGG ACAGTGAATA CTACAATTCC TTAAAATGGA TCTTAGAAAA CGACCCAACC
    GAGCTGGATC TCAGGTTTTG CATAGATGAA GAAAACTTCG GTCAGACGTA TCAGGTGGAT
    CTGAAGCCCA ATGGATCTGA GATGGTCGTG ACCAATGACA ACAAGCGAGA GTACATTGAC
    CTCGTGATCC AATGGAGGTT TGTCAACCGG GTACAGAAGC AGATGAATGC CTTTTTAGAA
    GGTTTTACTG AACTCATTCC TATTGATCTA ATAAAAATCT TTGATGAAAA TGAGCTGGAG
    TTGCTAATGT GCGGCCTTGG TGATGTTGAT GTGAATGACT GGAGGCAACA CACCCTGTAT
    AAGAACGGCT ACTGCCCCAA CCACCCCGCC ATACAGTGGT TCTGGAAGGC GGTGTTATTA
    ATGGATGCAG AGAAACGCAT ACGCTTGCTG CAGTTTGTGA CTGGGACGTC ACGTGTGCCT
    ATGAATGGAT TTGCTGAACT GTACGGTTCA AATGGTCCTC AGCTGTTTAC AATAGAGCAG
    TGGGGAAGCC CCGACAAGCT GCCCAGAGCT CACACATGCT TTAACCGCCT GGACTTACCT
    CCATACGACT CGTTTGAGGA TTTACGGGAG AAGCTTCTCA TGGCCGTGGA GAACGCTCAG
    GGATTCGAAG GCGTCGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815106.1
    CDS609..4169
    Translation

    Target ORF information:

    RefSeq Version XM_012959652.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959652.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGGCGCAA AAGACTTCCC CGTGCGCAGA
    GCAGTGAAGG ACACCCTGTC CAACCCACAG TCTCCACAGC CGTCACCTTA TAATTCTCCA
    AAGCCACAAC ACAAAGGGGC CCAGAGCTTC TTGCCGCCGG GCTGGGAGAT GAGAATAGCA
    CCCAATGGCA GACCATTCTT TATTGACCAT AACACAAAAA CTACCACATG GGAGGATCCG
    CGACTGAAGT TCCCAGTACA CATGAGAACA AAAGCTTCCT TGAACCCTAA TGATCTGGGT
    CCTCTCCCTC CGGGCTGGGA GGAAAGAATC CACGGGGATG GGCGAACGTT CTATATCGAT
    CACAGAAGTC CAGGGTTGAG GTGTCGAGAC ACAGGTACCA GCTGCTCGGT GCCCCCCTAC
    GACACCAAAA TAACCCAATG GGAGGACCCC CGGCTTCAGA ATCCCGCCAT CACTGGCCCT
    GCCGTCCCCT ACTCAAGAGA GTTTAAGCAG AAATACGACT ACTTCAGGAA GAAGTTGAAG
    AAACCTGCGG ATATTCCAAA TCGATTTGAG ATGAAGCTTC ACAGAAACAA CATTTTCGAG
    GAATCCTATA GGAGGATAAT GTCGGTGAAG CGGCCGGATG TCCTTAAAGC CAGATTATGG
    ATTGAATTTG AGTCGGAAAA AGGCCTCGAT TATGGAGGAG TAGCAAGGGA ATGGTTCTTC
    CTTCTGTCCA AAGAGATGTT CAACCCATAC TACGGGCTGT TTGAGTACTC CGCTACGGAC
    AATTACACAC TTCAGATAAA TCCCAATTCG GGTCTTTGCA ATGAAGATCA CCTTTCGTAC
    TTCACGTTTA TTGGTCGGAT TGCTGGGCTC GCCGTGTTCC ATGGAAAATT ATTAGATGGT
    TTCTTCATTA GACCATTCTA TAAGATGATG CTGGGGAAAC AAATAACGCT GAAAGACATG
    GAGTCAGTGG ACAGTGAATA CTACAATTCC TTAAAATGGA TCTTAGAAAA CGACCCAACC
    GAGCTGGATC TCAGGTTTTG CATAGATGAA GAAAACTTCG GTCAGACGTA TCAGGTGGAT
    CTGAAGCCCA ATGGATCTGA GATGGTCGTG ACCAATGACA ACAAGCGAGA GTACATTGAC
    CTCGTGATCC AATGGAGGTT TGTCAACCGG GTACAGAAGC AGATGAATGC CTTTTTAGAA
    GGTTTTACTG AACTCATTCC TATTGATCTA ATAAAAATCT TTGATGAAAA TGAGCTGGAG
    TTGCTAATGT GCGGCCTTGG TGATGTTGAT GTGAATGACT GGAGGCAACA CACCCTGTAT
    AAGAACGGCT ACTGCCCCAA CCACCCCGCC ATACAGTGGT TCTGGAAGGC GGTGTTATTA
    ATGGATGCAG AGAAACGCAT ACGCTTGCTG CAGTTTGTGA CTGGGACGTC ACGTGTGCCT
    ATGAATGGAT TTGCTGAACT GTACGGTTCA AATGGTCCTC AGCTGTTTAC AATAGAGCAG
    TGGGGAAGCC CCGACAAGCT GCCCAGAGCT CACACATGCT TTAACCGCCT GGACTTACCT
    CCATACGACT CGTTTGAGGA TTTACGGGAG AAGCTTCTCA TGGCCGTGGA GAACGCTCAG
    GGATTCGAAG GCGTCGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47771
    Clone ID Related Accession (Same CDS sequence) XM_031897897.1
    Accession Version XM_031897897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG GGCGCAAAAG ACTTCCCCGT GCGCAGAGCA
    GTGAAGGACA CCCTGTCCAA CCCACAGTCT CCACAGCCGT CACCTTATAA TTCTCCAAAG
    CCACAACACA AAGGGGCCCA GAGCTTCTTG CCGCCGGGCT GGGAGATGAG AATAGCACCC
    AATGGCAGAC CATTCTTTAT TGACCATAAC ACAAAAACTA CCACATGGGA GGATCCGCGA
    CTGAAGTTCC CAGTACACAT GAGAACAAAA GCTTCCTTGA ACCCTAATGA TCTGGGTCCT
    CTCCCTCCGG GCTGGGAGGA AAGAATCCAC GGGGATGGGC GAACGTTCTA TATCGATCAC
    AGAAGTCCAG GGTTGAGGTG TCGAGACACA GGTACCAGCT GCTCGGTGCC CCCCTACGAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTTGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753757.1
    CDS609..4166
    Translation

    Target ORF information:

    RefSeq Version XM_031897897.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG GGCGCAAAAG ACTTCCCCGT GCGCAGAGCA
    GTGAAGGACA CCCTGTCCAA CCCACAGTCT CCACAGCCGT CACCTTATAA TTCTCCAAAG
    CCACAACACA AAGGGGCCCA GAGCTTCTTG CCGCCGGGCT GGGAGATGAG AATAGCACCC
    AATGGCAGAC CATTCTTTAT TGACCATAAC ACAAAAACTA CCACATGGGA GGATCCGCGA
    CTGAAGTTCC CAGTACACAT GAGAACAAAA GCTTCCTTGA ACCCTAATGA TCTGGGTCCT
    CTCCCTCCGG GCTGGGAGGA AAGAATCCAC GGGGATGGGC GAACGTTCTA TATCGATCAC
    AGAAGTCCAG GGTTGAGGTG TCGAGACACA GGTACCAGCT GCTCGGTGCC CCCCTACGAC
    ACCAAAATAA CCCAATGGGA GGACCCCCGG CTTCAGAATC CCGCCATCAC TGGCCCTGCC
    GTCCCCTACT CAAGAGAGTT TAAGCAGAAA TACGACTACT TCAGGAAGAA GTTGAAGAAA
    CCTGCGGATA TTCCAAATCG ATTTGAGATG AAGCTTCACA GAAACAACAT TTTCGAGGAA
    TCCTATAGGA GGATAATGTC GGTGAAGCGG CCGGATGTCC TTAAAGCCAG ATTATGGATT
    GAATTTGAGT CGGAAAAAGG CCTCGATTAT GGAGGAGTAG CAAGGGAATG GTTCTTCCTT
    CTGTCCAAAG AGATGTTCAA CCCATACTAC GGGCTGTTTG AGTACTCCGC TACGGACAAT
    TACACACTTC AGATAAATCC CAATTCGGGT CTTTGCAATG AAGATCACCT TTCGTACTTC
    ACGTTTATTG GTCGGATTGC TGGGCTCGCC GTGTTCCATG GAAAATTATT AGATGGTTTC
    TTCATTAGAC CATTCTATAA GATGATGCTG GGGAAACAAA TAACGCTGAA AGACATGGAG
    TCAGTGGACA GTGAATACTA CAATTCCTTA AAATGGATCT TAGAAAACGA CCCAACCGAG
    CTGGATCTCA GGTTTTGCAT AGATGAAGAA AACTTCGGTC AGACGTATCA GGTGGATCTG
    AAGCCCAATG GATCTGAGAT GGTCGTGACC AATGACAACA AGCGAGAGTA CATTGACCTC
    GTGATCCAAT GGAGGTTTGT CAACCGGGTA CAGAAGCAGA TGAATGCCTT TTTAGAAGGT
    TTTACTGAAC TCATTCCTAT TGATCTAATA AAAATCTTTG ATGAAAATGA GCTGGAGTTG
    CTAATGTGCG GCCTTGGTGA TGTTGATGTG AATGACTGGA GGCAACACAC CCTGTATAAG
    AACGGCTACT GCCCCAACCA CCCCGCCATA CAGTGGTTCT GGAAGGCGGT GTTATTAATG
    GATGCAGAGA AACGCATACG CTTGCTGCAG TTTGTGACTG GGACGTCACG TGTGCCTATG
    AATGGATTTG CTGAACTGTA CGGTTCAAAT GGTCCTCAGC TGTTTACAAT AGAGCAGTGG
    GGAAGCCCCG ACAAGCTGCC CAGAGCTCAC ACATGCTTTA ACCGCCTGGA CTTACCTCCA
    TACGACTCGT TTGAGGATTT ACGGGAGAAG CTTCTCATGG CCGTGGAGAA CGCTCAGGGA
    TTCGAAGGCG TCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47772
    Clone ID Related Accession (Same CDS sequence) XM_012959653.3
    Accession Version XM_012959653.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTTGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815107.1
    CDS609..4145
    Translation

    Target ORF information:

    RefSeq Version XM_012959653.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959653.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTTGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47773
    Clone ID Related Accession (Same CDS sequence) XM_012959655.3
    Accession Version XM_012959655.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTT
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815109.1
    CDS609..4142
    Translation

    Target ORF information:

    RefSeq Version XM_012959655.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959655.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTT
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47774
    Clone ID Related Accession (Same CDS sequence) XM_031897901.1
    Accession Version XM_031897901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753761.1
    CDS609..3971
    Translation

    Target ORF information:

    RefSeq Version XM_031897901.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGAGGATC CGCGACTGAA GTTCCCAGTA
    CACATGAGAA CAAAAGCTTC CTTGAACCCT AATGATCTGG GTCCTCTCCC TCCGGGCTGG
    GAGGAAAGAA TCCACGGGGA TGGGCGAACG TTCTATATCG ATCACAGAAG TCCAGGGTTG
    AGGTGTCGAG ACACAGGTAC CAGCTGCTCG GTGCCCCCCT ACGACACCAA AATAACCCAA
    TGGGAGGACC CCCGGCTTCA GAATCCCGCC ATCACTGGCC CTGCCGTCCC CTACTCAAGA
    GAGTTTAAGC AGAAATACGA CTACTTCAGG AAGAAGTTGA AGAAACCTGC GGATATTCCA
    AATCGATTTG AGATGAAGCT TCACAGAAAC AACATTTTCG AGGAATCCTA TAGGAGGATA
    ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA GCCAGATTAT GGATTGAATT TGAGTCGGAA
    AAAGGCCTCG ATTATGGAGG AGTAGCAAGG GAATGGTTCT TCCTTCTGTC CAAAGAGATG
    TTCAACCCAT ACTACGGGCT GTTTGAGTAC TCCGCTACGG ACAATTACAC ACTTCAGATA
    AATCCCAATT CGGGTCTTTG CAATGAAGAT CACCTTTCGT ACTTCACGTT TATTGGTCGG
    ATTGCTGGGC TCGCCGTGTT CCATGGAAAA TTATTAGATG GTTTCTTCAT TAGACCATTC
    TATAAGATGA TGCTGGGGAA ACAAATAACG CTGAAAGACA TGGAGTCAGT GGACAGTGAA
    TACTACAATT CCTTAAAATG GATCTTAGAA AACGACCCAA CCGAGCTGGA TCTCAGGTTT
    TGCATAGATG AAGAAAACTT CGGTCAGACG TATCAGGTGG ATCTGAAGCC CAATGGATCT
    GAGATGGTCG TGACCAATGA CAACAAGCGA GAGTACATTG ACCTCGTGAT CCAATGGAGG
    TTTGTCAACC GGGTACAGAA GCAGATGAAT GCCTTTTTAG AAGGTTTTAC TGAACTCATT
    CCTATTGATC TAATAAAAAT CTTTGATGAA AATGAGCTGG AGTTGCTAAT GTGCGGCCTT
    GGTGATGTTG ATGTGAATGA CTGGAGGCAA CACACCCTGT ATAAGAACGG CTACTGCCCC
    AACCACCCCG CCATACAGTG GTTCTGGAAG GCGGTGTTAT TAATGGATGC AGAGAAACGC
    ATACGCTTGC TGCAGTTTGT GACTGGGACG TCACGTGTGC CTATGAATGG ATTTGCTGAA
    CTGTACGGTT CAAATGGTCC TCAGCTGTTT ACAATAGAGC AGTGGGGAAG CCCCGACAAG
    CTGCCCAGAG CTCACACATG CTTTAACCGC CTGGACTTAC CTCCATACGA CTCGTTTGAG
    GATTTACGGG AGAAGCTTCT CATGGCCGTG GAGAACGCTC AGGGATTCGA AGGCGTCGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47775
    Clone ID Related Accession (Same CDS sequence) XM_031897902.1
    Accession Version XM_031897902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753762.1
    CDS609..3968
    Translation

    Target ORF information:

    RefSeq Version XM_031897902.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGATCCGC GACTGAAGTT CCCAGTACAC
    ATGAGAACAA AAGCTTCCTT GAACCCTAAT GATCTGGGTC CTCTCCCTCC GGGCTGGGAG
    GAAAGAATCC ACGGGGATGG GCGAACGTTC TATATCGATC ACAGAAGTCC AGGGTTGAGG
    TGTCGAGACA CAGGTACCAG CTGCTCGGTG CCCCCCTACG ACACCAAAAT AACCCAATGG
    GAGGACCCCC GGCTTCAGAA TCCCGCCATC ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG
    TTTAAGCAGA AATACGACTA CTTCAGGAAG AAGTTGAAGA AACCTGCGGA TATTCCAAAT
    CGATTTGAGA TGAAGCTTCA CAGAAACAAC ATTTTCGAGG AATCCTATAG GAGGATAATG
    TCGGTGAAGC GGCCGGATGT CCTTAAAGCC AGATTATGGA TTGAATTTGA GTCGGAAAAA
    GGCCTCGATT ATGGAGGAGT AGCAAGGGAA TGGTTCTTCC TTCTGTCCAA AGAGATGTTC
    AACCCATACT ACGGGCTGTT TGAGTACTCC GCTACGGACA ATTACACACT TCAGATAAAT
    CCCAATTCGG GTCTTTGCAA TGAAGATCAC CTTTCGTACT TCACGTTTAT TGGTCGGATT
    GCTGGGCTCG CCGTGTTCCA TGGAAAATTA TTAGATGGTT TCTTCATTAG ACCATTCTAT
    AAGATGATGC TGGGGAAACA AATAACGCTG AAAGACATGG AGTCAGTGGA CAGTGAATAC
    TACAATTCCT TAAAATGGAT CTTAGAAAAC GACCCAACCG AGCTGGATCT CAGGTTTTGC
    ATAGATGAAG AAAACTTCGG TCAGACGTAT CAGGTGGATC TGAAGCCCAA TGGATCTGAG
    ATGGTCGTGA CCAATGACAA CAAGCGAGAG TACATTGACC TCGTGATCCA ATGGAGGTTT
    GTCAACCGGG TACAGAAGCA GATGAATGCC TTTTTAGAAG GTTTTACTGA ACTCATTCCT
    ATTGATCTAA TAAAAATCTT TGATGAAAAT GAGCTGGAGT TGCTAATGTG CGGCCTTGGT
    GATGTTGATG TGAATGACTG GAGGCAACAC ACCCTGTATA AGAACGGCTA CTGCCCCAAC
    CACCCCGCCA TACAGTGGTT CTGGAAGGCG GTGTTATTAA TGGATGCAGA GAAACGCATA
    CGCTTGCTGC AGTTTGTGAC TGGGACGTCA CGTGTGCCTA TGAATGGATT TGCTGAACTG
    TACGGTTCAA ATGGTCCTCA GCTGTTTACA ATAGAGCAGT GGGGAAGCCC CGACAAGCTG
    CCCAGAGCTC ACACATGCTT TAACCGCCTG GACTTACCTC CATACGACTC GTTTGAGGAT
    TTACGGGAGA AGCTTCTCAT GGCCGTGGAG AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47776
    Clone ID Related Accession (Same CDS sequence) XM_031897907.1
    Accession Version XM_031897907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753767.1
    CDS609..3947
    Translation

    Target ORF information:

    RefSeq Version XM_031897907.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTTGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47777
    Clone ID Related Accession (Same CDS sequence) XM_031897913.1
    Accession Version XM_031897913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGAGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAG AAGTCCAGGG TTGAGGTGTC GAGACACAGG TACCAGCTGC
    TCGGTGCCCC CCTACGACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TTGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753773.1
    CDS609..3944
    Translation

    Target ORF information:

    RefSeq Version XM_031897913.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGAGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAG AAGTCCAGGG TTGAGGTGTC GAGACACAGG TACCAGCTGC
    TCGGTGCCCC CCTACGACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TTGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47778
    Clone ID Related Accession (Same CDS sequence) XM_031897914.1
    Accession Version XM_031897914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753774.1
    CDS609..3941
    Translation

    Target ORF information:

    RefSeq Version XM_031897914.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 like E3 ubiquitin protein ligase (nedd4l), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTCGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTTG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26555
    Clone ID Related Accession (Same CDS sequence) XM_012959594.2
    Accession Version XM_012959594.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815048.1
    CDS609..4481
    Translation

    Target ORF information:

    RefSeq Version XM_012959594.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959594.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26556
    Clone ID Related Accession (Same CDS sequence) XM_012959595.2
    Accession Version XM_012959595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815049.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959595.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26557
    Clone ID Related Accession (Same CDS sequence) XM_012959597.2
    Accession Version XM_012959597.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    GCCTGGGATG TGGTGGACTC CAATGATTCA AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT
    CCACCTATGC CGCCGGGATG GGAAGAAAAA GTGGACAACC TTGGGCGGAC ATATTACGTC
    AACCATAATA ACAAGACCAC TCAGTGGCAA AGGCCAAGCC TTGTAGACGT TACGTCCGAA
    TCGGACAGCA ACATTCGGCA CATACAGCAA GAGGCCCACC GAGTGTTCCG ATCTCGGAGA
    CATATCAGTG AAGACCTAGA ACCCGACCAT ATAGAAGGAG GAGCGGATGT TCCAGAGCCA
    TGGGAAACCA TTTCCGAGGA AATGACTTTG ACGACCGACA CGTTAAACCA GTCTCTCCCA
    CCCCCTGCTT CCCCGGATTC TCGGGCCAGC GCTCTGGAAC TGTCCGAGGA GCTGAACAGG
    AGGCTGCAGA TTAGCTCCGA GTCCAACGGA GAACAGTTCA GTTCTATCAT TCAAACAGCT
    TGGGAGAGGC AAAAGCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815051.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959597.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959597.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    GCCTGGGATG TGGTGGACTC CAATGATTCA AGCTCTCCAC ATCAGCAAGA GCTCCCAGCT
    CCACCTATGC CGCCGGGATG GGAAGAAAAA GTGGACAACC TTGGGCGGAC ATATTACGTC
    AACCATAATA ACAAGACCAC TCAGTGGCAA AGGCCAAGCC TTGTAGACGT TACGTCCGAA
    TCGGACAGCA ACATTCGGCA CATACAGCAA GAGGCCCACC GAGTGTTCCG ATCTCGGAGA
    CATATCAGTG AAGACCTAGA ACCCGACCAT ATAGAAGGAG GAGCGGATGT TCCAGAGCCA
    TGGGAAACCA TTTCCGAGGA AATGACTTTG ACGACCGACA CGTTAAACCA GTCTCTCCCA
    CCCCCTGCTT CCCCGGATTC TCGGGCCAGC GCTCTGGAAC TGTCCGAGGA GCTGAACAGG
    AGGCTGCAGA TTAGCTCCGA GTCCAACGGA GAACAGTTCA GTTCTATCAT TCAAACAGCT
    TGGGAGAGGC AAAAGCAGAG AGAACCGTCC ACGAGATTAA GGTCTTGCAG TGTAACAGAT
    GGTGTCGCCG AGCAGGCTCA TTTATCACTG CCGAGCGTCT CAACGGGAAG AGCTCGTTCC
    TCAACTGTCA CAGGCGGTGA GGATTCTACG CCAACCGTGG CCTATGTGCA CACCACTCCG
    GGTTTGCCTT CAGGATGGGA GGAGAGGAAA GACGCTAAAG GCCGTACCTA TTATGTCAAT
    CATAACAATC GCACCACCAC ATGGACCAGA CCTATTGTGC AGCACGCCGA GGACGGGGCA
    GTGGGATCTA CTTCAAACAG TAGTAACCAT CTGAGCGAGC CTCAGATCCG ACGGCCTCGT
    AGTCTCAGTT CTCCCACAGT TACCCTATCT GCACCACTCG AGGGCGCAAA AGACTTCCCC
    GTGCGCAGAG CAGTGAAGGA CACCCTGTCC AACCCACAGT CTCCACAGCC GTCACCTTAT
    AATTCTCCAA AGCCACAACA CAAAGGGGCC CAGAGCTTCT TGCCGCCGGG CTGGGAGATG
    AGAATAGCAC CCAATGGCAG ACCATTCTTT ATTGACCATA ACACAAAAAC TACCACATGG
    GAGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26558
    Clone ID Related Accession (Same CDS sequence) XM_012959602.2
    Accession Version XM_012959602.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815056.1
    CDS609..4478
    Translation

    Target ORF information:

    RefSeq Version XM_012959602.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959602.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAGAAGTCC AGGGTTGAGG TGTCGAGACA CAGGTACCAG CTGCTCGGTG
    CCCCCCTACG ACACCAAAAT AACCCAATGG GAGGACCCCC GGCTTCAGAA TCCCGCCATC
    ACTGGCCCTG CCGTCCCCTA CTCAAGAGAG TTTAAGCAGA AATACGACTA CTTCAGGAAG
    AAGTTGAAGA AACCTGCGGA TATTCCAAAT CGATTTGAGA TGAAGCTTCA CAGAAACAAC
    ATTTTCGAGG AATCCTATAG GAGGATAATG TCGGTGAAGC GGCCGGATGT CCTTAAAGCC
    AGATTATGGA TTGAATTTGA GTCGGAAAAA GGCCTCGATT ATGGAGGAGT AGCAAGGGAA
    TGGTTCTTCC TTCTGTCCAA AGAGATGTTC AACCCATACT ACGGGCTGTT TGAGTACTCC
    GCTACGGACA ATTACACACT TCAGATAAAT CCCAATTCGG GTCTTTGCAA TGAAGATCAC
    CTTTCGTACT TCACGTTTAT TGGTCGGATT GCTGGGCTCG CCGTGTTCCA TGGAAAATTA
    TTAGATGGTT TCTTCATTAG ACCATTCTAT AAGATGATGC TGGGGAAACA AATAACGCTG
    AAAGACATGG AGTCAGTGGA CAGTGAATAC TACAATTCCT TAAAATGGAT CTTAGAAAAC
    GACCCAACCG AGCTGGATCT CAGGTTTTGC ATAGATGAAG AAAACTTCGG TCAGACGTAT
    CAGGTGGATC TGAAGCCCAA TGGATCTGAG ATGGTCGTGA CCAATGACAA CAAGCGAGAG
    TACATTGACC TCGTGATCCA ATGGAGGTTT GTCAACCGGG TACAGAAGCA GATGAATGCC
    TTTTTAGAAG GTTTTACTGA ACTCATTCCT ATTGATCTAA TAAAAATCTT TGATGAAAAT
    GAGCTGGAGT TGCTAATGTG CGGCCTTGGT GATGTCGATG TGAATGACTG GAGGCAACAC
    ACCCTGTATA AGAACGGCTA CTGCCCCAAC CACCCCGCCA TACAGTGGTT CTGGAAGGCG
    GTGTTATTAA TGGATGCAGA GAAACGCATA CGCTTGCTGC AGTTTGTGAC TGGGACGTCA
    CGTGTGCCTA TGAATGGATT TGCTGAACTG TACGGTTCAA ATGGTCCTCA GCTGTTTACA
    ATAGAGCAGT GGGGAAGCCC CGACAAGCTG CCCAGAGCTC ACACATGCTT TAACCGCCTG
    GACTTACCTC CATACGACTC GTTTGAGGAT TTACGGGAGA AGCTTCTCAT GGCCGTGGAG
    AACGCTCAGG GATTCGAAGG CGTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26559
    Clone ID Related Accession (Same CDS sequence) XM_012959606.2
    Accession Version XM_012959606.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815060.1
    CDS609..4457
    Translation

    Target ORF information:

    RefSeq Version XM_012959606.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959606.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26560
    Clone ID Related Accession (Same CDS sequence) XM_012959611.2
    Accession Version XM_012959611.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TCGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815065.1
    CDS609..4454
    Translation

    Target ORF information:

    RefSeq Version XM_012959611.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TCGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26561
    Clone ID Related Accession (Same CDS sequence) XM_012959617.2
    Accession Version XM_012959617.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815071.1
    CDS609..4424
    Translation

    Target ORF information:

    RefSeq Version XM_012959617.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26562
    Clone ID Related Accession (Same CDS sequence) XM_012959625.2
    Accession Version XM_012959625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815079.1
    CDS609..4421
    Translation

    Target ORF information:

    RefSeq Version XM_012959625.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCGAGCG TCTCAACGGG AAGAGCTCGT
    TCCTCAACTG TCACAGGCGG TGAGGATTCT ACGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGATCCGC GACTGAAGTT CCCAGTACAC ATGAGAACAA AAGCTTCCTT GAACCCTAAT
    GATCTGGGTC CTCTCCCTCC GGGCTGGGAG GAAAGAATCC ACGGGGATGG GCGAACGTTC
    TATATCGATC ACAACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26563
    Clone ID Related Accession (Same CDS sequence) XM_012959632.2
    Accession Version XM_012959632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815086.1
    CDS609..4421
    Translation

    Target ORF information:

    RefSeq Version XM_012959632.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGCCAACCG TGGCCTATGT GCACACCACT
    CCGGGTTTGC CTTCAGGATG GGAGGAGAGG AAAGACGCTA AAGGCCGTAC CTATTATGTC
    AATCATAACA ATCGCACCAC CACATGGACC AGACCTATTG TGCAGCACGC CGAGGACGGG
    GCAGTGGGAT CTACTTCAAA CAGTAGTAAC CATCTGAGCG AGCCTCAGAT CCGACGGCCT
    CGTAGTCTCA GTTCTCCCAC AGTTACCCTA TCTGCACCAC TCGAGGGCGC AAAAGACTTC
    CCCGTGCGCA GAGCAGTGAA GGACACCCTG TCCAACCCAC AGTCTCCACA GCCGTCACCT
    TATAATTCTC CAAAGCCACA ACACAAAGGG GCCCAGAGCT TCTTGCCGCC GGGCTGGGAG
    ATGAGAATAG CACCCAATGG CAGACCATTC TTTATTGACC ATAACACAAA AACTACCACA
    TGGGAGGATC CGCGACTGAA GTTCCCAGTA CACATGAGAA CAAAAGCTTC CTTGAACCCT
    AATGATCTGG GTCCTCTCCC TCCGGGCTGG GAGGAAAGAA TCCACGGGGA TGGGCGAACG
    TTCTATATCG ATCACAGAAG TCCAGGGTTG AGGTGTCGAG ACACAGGTAC CAGCTGCTCG
    GTGCCCCCCT ACGACACCAA AATAACCCAA TGGGAGGACC CCCGGCTTCA GAATCCCGCC
    ATCACTGGCC CTGCCGTCCC CTACTCAAGA GAGTTTAAGC AGAAATACGA CTACTTCAGG
    AAGAAGTTGA AGAAACCTGC GGATATTCCA AATCGATTTG AGATGAAGCT TCACAGAAAC
    AACATTTTCG AGGAATCCTA TAGGAGGATA ATGTCGGTGA AGCGGCCGGA TGTCCTTAAA
    GCCAGATTAT GGATTGAATT TGAGTCGGAA AAAGGCCTCG ATTATGGAGG AGTAGCAAGG
    GAATGGTTCT TCCTTCTGTC CAAAGAGATG TTCAACCCAT ACTACGGGCT GTTTGAGTAC
    TCCGCTACGG ACAATTACAC ACTTCAGATA AATCCCAATT CGGGTCTTTG CAATGAAGAT
    CACCTTTCGT ACTTCACGTT TATTGGTCGG ATTGCTGGGC TCGCCGTGTT CCATGGAAAA
    TTATTAGATG GTTTCTTCAT TAGACCATTC TATAAGATGA TGCTGGGGAA ACAAATAACG
    CTGAAAGACA TGGAGTCAGT GGACAGTGAA TACTACAATT CCTTAAAATG GATCTTAGAA
    AACGACCCAA CCGAGCTGGA TCTCAGGTTT TGCATAGATG AAGAAAACTT CGGTCAGACG
    TATCAGGTGG ATCTGAAGCC CAATGGATCT GAGATGGTCG TGACCAATGA CAACAAGCGA
    GAGTACATTG ACCTCGTGAT CCAATGGAGG TTTGTCAACC GGGTACAGAA GCAGATGAAT
    GCCTTTTTAG AAGGTTTTAC TGAACTCATT CCTATTGATC TAATAAAAAT CTTTGATGAA
    AATGAGCTGG AGTTGCTAAT GTGCGGCCTT GGTGATGTCG ATGTGAATGA CTGGAGGCAA
    CACACCCTGT ATAAGAACGG CTACTGCCCC AACCACCCCG CCATACAGTG GTTCTGGAAG
    GCGGTGTTAT TAATGGATGC AGAGAAACGC ATACGCTTGC TGCAGTTTGT GACTGGGACG
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTGTACGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AGTGGGGAAG CCCCGACAAG CTGCCCAGAG CTCACACATG CTTTAACCGC
    CTGGACTTAC CTCCATACGA CTCGTTTGAG GATTTACGGG AGAAGCTTCT CATGGCCGTG
    GAGAACGCTC AGGGATTCGA AGGCGTCGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26564
    Clone ID Related Accession (Same CDS sequence) XM_012959635.2
    Accession Version XM_012959635.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815089.1
    CDS609..4400
    Translation

    Target ORF information:

    RefSeq Version XM_012959635.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959635.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CGAGCGTCTC AACGGGAAGA GCTCGTTCCT CAACTGTCAC AGGCGGTGAG
    GATTCTACGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26565
    Clone ID Related Accession (Same CDS sequence) XM_012959639.2
    Accession Version XM_012959639.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815093.1
    CDS609..4397
    Translation

    Target ORF information:

    RefSeq Version XM_012959639.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959639.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCGA GCGTCTCAAC GGGAAGAGCT CGTTCCTCAA CTGTCACAGG CGGTGAGGAT
    TCTACGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26566
    Clone ID Related Accession (Same CDS sequence) XM_012959640.2
    Accession Version XM_012959640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815094.1
    CDS609..4397
    Translation

    Target ORF information:

    RefSeq Version XM_012959640.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAGAAGTCCA
    GGGTTGAGGT GTCGAGACAC AGGTACCAGC TGCTCGGTGC CCCCCTACGA CACCAAAATA
    ACCCAATGGG AGGACCCCCG GCTTCAGAAT CCCGCCATCA CTGGCCCTGC CGTCCCCTAC
    TCAAGAGAGT TTAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT
    ATTCCAAATC GATTTGAGAT GAAGCTTCAC AGAAACAACA TTTTCGAGGA ATCCTATAGG
    AGGATAATGT CGGTGAAGCG GCCGGATGTC CTTAAAGCCA GATTATGGAT TGAATTTGAG
    TCGGAAAAAG GCCTCGATTA TGGAGGAGTA GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA
    GAGATGTTCA ACCCATACTA CGGGCTGTTT GAGTACTCCG CTACGGACAA TTACACACTT
    CAGATAAATC CCAATTCGGG TCTTTGCAAT GAAGATCACC TTTCGTACTT CACGTTTATT
    GGTCGGATTG CTGGGCTCGC CGTGTTCCAT GGAAAATTAT TAGATGGTTT CTTCATTAGA
    CCATTCTATA AGATGATGCT GGGGAAACAA ATAACGCTGA AAGACATGGA GTCAGTGGAC
    AGTGAATACT ACAATTCCTT AAAATGGATC TTAGAAAACG ACCCAACCGA GCTGGATCTC
    AGGTTTTGCA TAGATGAAGA AAACTTCGGT CAGACGTATC AGGTGGATCT GAAGCCCAAT
    GGATCTGAGA TGGTCGTGAC CAATGACAAC AAGCGAGAGT ACATTGACCT CGTGATCCAA
    TGGAGGTTTG TCAACCGGGT ACAGAAGCAG ATGAATGCCT TTTTAGAAGG TTTTACTGAA
    CTCATTCCTA TTGATCTAAT AAAAATCTTT GATGAAAATG AGCTGGAGTT GCTAATGTGC
    GGCCTTGGTG ATGTCGATGT GAATGACTGG AGGCAACACA CCCTGTATAA GAACGGCTAC
    TGCCCCAACC ACCCCGCCAT ACAGTGGTTC TGGAAGGCGG TGTTATTAAT GGATGCAGAG
    AAACGCATAC GCTTGCTGCA GTTTGTGACT GGGACGTCAC GTGTGCCTAT GAATGGATTT
    GCTGAACTGT ACGGTTCAAA TGGTCCTCAG CTGTTTACAA TAGAGCAGTG GGGAAGCCCC
    GACAAGCTGC CCAGAGCTCA CACATGCTTT AACCGCCTGG ACTTACCTCC ATACGACTCG
    TTTGAGGATT TACGGGAGAA GCTTCTCATG GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC
    GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26567
    Clone ID Related Accession (Same CDS sequence) XM_012959645.2
    Accession Version XM_012959645.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TCGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815099.1
    CDS609..4394
    Translation

    Target ORF information:

    RefSeq Version XM_012959645.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959645.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGCCAA CCGTGGCCTA TGTGCACACC ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG
    AGGAAAGACG CTAAAGGCCG TACCTATTAT GTCAATCATA ACAATCGCAC CACCACATGG
    ACCAGACCTA TTGTGCAGCA CGCCGAGGAC GGGGCAGTGG GATCTACTTC AAACAGTAGT
    AACCATCTGA GCGAGCCTCA GATCCGACGG CCTCGTAGTC TCAGTTCTCC CACAGTTACC
    CTATCTGCAC CACTCGAGGG CGCAAAAGAC TTCCCCGTGC GCAGAGCAGT GAAGGACACC
    CTGTCCAACC CACAGTCTCC ACAGCCGTCA CCTTATAATT CTCCAAAGCC ACAACACAAA
    GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG GAGATGAGAA TAGCACCCAA TGGCAGACCA
    TTCTTTATTG ACCATAACAC AAAAACTACC ACATGGGAGG ATCCGCGACT GAAGTTCCCA
    GTACACATGA GAACAAAAGC TTCCTTGAAC CCTAATGATC TGGGTCCTCT CCCTCCGGGC
    TGGGAGGAAA GAATCCACGG GGATGGGCGA ACGTTCTATA TCGATCACAG AAGTCCAGGG
    TTGAGGTGTC GAGACACAGG TACCAGCTGC TCGGTGCCCC CCTACGACAC CAAAATAACC
    CAATGGGAGG ACCCCCGGCT TCAGAATCCC GCCATCACTG GCCCTGCCGT CCCCTACTCA
    AGAGAGTTTA AGCAGAAATA CGACTACTTC AGGAAGAAGT TGAAGAAACC TGCGGATATT
    CCAAATCGAT TTGAGATGAA GCTTCACAGA AACAACATTT TCGAGGAATC CTATAGGAGG
    ATAATGTCGG TGAAGCGGCC GGATGTCCTT AAAGCCAGAT TATGGATTGA ATTTGAGTCG
    GAAAAAGGCC TCGATTATGG AGGAGTAGCA AGGGAATGGT TCTTCCTTCT GTCCAAAGAG
    ATGTTCAACC CATACTACGG GCTGTTTGAG TACTCCGCTA CGGACAATTA CACACTTCAG
    ATAAATCCCA ATTCGGGTCT TTGCAATGAA GATCACCTTT CGTACTTCAC GTTTATTGGT
    CGGATTGCTG GGCTCGCCGT GTTCCATGGA AAATTATTAG ATGGTTTCTT CATTAGACCA
    TTCTATAAGA TGATGCTGGG GAAACAAATA ACGCTGAAAG ACATGGAGTC AGTGGACAGT
    GAATACTACA ATTCCTTAAA ATGGATCTTA GAAAACGACC CAACCGAGCT GGATCTCAGG
    TTTTGCATAG ATGAAGAAAA CTTCGGTCAG ACGTATCAGG TGGATCTGAA GCCCAATGGA
    TCTGAGATGG TCGTGACCAA TGACAACAAG CGAGAGTACA TTGACCTCGT GATCCAATGG
    AGGTTTGTCA ACCGGGTACA GAAGCAGATG AATGCCTTTT TAGAAGGTTT TACTGAACTC
    ATTCCTATTG ATCTAATAAA AATCTTTGAT GAAAATGAGC TGGAGTTGCT AATGTGCGGC
    CTTGGTGATG TCGATGTGAA TGACTGGAGG CAACACACCC TGTATAAGAA CGGCTACTGC
    CCCAACCACC CCGCCATACA GTGGTTCTGG AAGGCGGTGT TATTAATGGA TGCAGAGAAA
    CGCATACGCT TGCTGCAGTT TGTGACTGGG ACGTCACGTG TGCCTATGAA TGGATTTGCT
    GAACTGTACG GTTCAAATGG TCCTCAGCTG TTTACAATAG AGCAGTGGGG AAGCCCCGAC
    AAGCTGCCCA GAGCTCACAC ATGCTTTAAC CGCCTGGACT TACCTCCATA CGACTCGTTT
    GAGGATTTAC GGGAGAAGCT TCTCATGGCC GTGGAGAACG CTCAGGGATT CGAAGGCGTC
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26568
    Clone ID Related Accession (Same CDS sequence) XM_012959649.2
    Accession Version XM_012959649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815103.1
    CDS609..4340
    Translation

    Target ORF information:

    RefSeq Version XM_012959649.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGC CAACCGTGGC CTATGTGCAC ACCACTCCGG GTTTGCCTTC AGGATGGGAG
    GAGAGGAAAG ACGCTAAAGG CCGTACCTAT TATGTCAATC ATAACAATCG CACCACCACA
    TGGACCAGAC CTATTGTGCA GCACGCCGAG GACGGGGCAG TGGGATCTAC TTCAAACAGT
    AGTAACCATC TGAGCGAGCC TCAGATCCGA CGGCCTCGTA GTCTCAGTTC TCCCACAGTT
    ACCCTATCTG CACCACTCGA GGGCGCAAAA GACTTCCCCG TGCGCAGAGC AGTGAAGGAC
    ACCCTGTCCA ACCCACAGTC TCCACAGCCG TCACCTTATA ATTCTCCAAA GCCACAACAC
    AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC TGGGAGATGA GAATAGCACC CAATGGCAGA
    CCATTCTTTA TTGACCATAA CACAAAAACT ACCACATGGG AGGATCCGCG ACTGAAGTTC
    CCAGTACACA TGAGAACAAA AGCTTCCTTG AACCCTAATG ATCTGGGTCC TCTCCCTCCG
    GGCTGGGAGG AAAGAATCCA CGGGGATGGG CGAACGTTCT ATATCGATCA CAACACCAAA
    ATAACCCAAT GGGAGGACCC CCGGCTTCAG AATCCCGCCA TCACTGGCCC TGCCGTCCCC
    TACTCAAGAG AGTTTAAGCA GAAATACGAC TACTTCAGGA AGAAGTTGAA GAAACCTGCG
    GATATTCCAA ATCGATTTGA GATGAAGCTT CACAGAAACA ACATTTTCGA GGAATCCTAT
    AGGAGGATAA TGTCGGTGAA GCGGCCGGAT GTCCTTAAAG CCAGATTATG GATTGAATTT
    GAGTCGGAAA AAGGCCTCGA TTATGGAGGA GTAGCAAGGG AATGGTTCTT CCTTCTGTCC
    AAAGAGATGT TCAACCCATA CTACGGGCTG TTTGAGTACT CCGCTACGGA CAATTACACA
    CTTCAGATAA ATCCCAATTC GGGTCTTTGC AATGAAGATC ACCTTTCGTA CTTCACGTTT
    ATTGGTCGGA TTGCTGGGCT CGCCGTGTTC CATGGAAAAT TATTAGATGG TTTCTTCATT
    AGACCATTCT ATAAGATGAT GCTGGGGAAA CAAATAACGC TGAAAGACAT GGAGTCAGTG
    GACAGTGAAT ACTACAATTC CTTAAAATGG ATCTTAGAAA ACGACCCAAC CGAGCTGGAT
    CTCAGGTTTT GCATAGATGA AGAAAACTTC GGTCAGACGT ATCAGGTGGA TCTGAAGCCC
    AATGGATCTG AGATGGTCGT GACCAATGAC AACAAGCGAG AGTACATTGA CCTCGTGATC
    CAATGGAGGT TTGTCAACCG GGTACAGAAG CAGATGAATG CCTTTTTAGA AGGTTTTACT
    GAACTCATTC CTATTGATCT AATAAAAATC TTTGATGAAA ATGAGCTGGA GTTGCTAATG
    TGCGGCCTTG GTGATGTCGA TGTGAATGAC TGGAGGCAAC ACACCCTGTA TAAGAACGGC
    TACTGCCCCA ACCACCCCGC CATACAGTGG TTCTGGAAGG CGGTGTTATT AATGGATGCA
    GAGAAACGCA TACGCTTGCT GCAGTTTGTG ACTGGGACGT CACGTGTGCC TATGAATGGA
    TTTGCTGAAC TGTACGGTTC AAATGGTCCT CAGCTGTTTA CAATAGAGCA GTGGGGAAGC
    CCCGACAAGC TGCCCAGAGC TCACACATGC TTTAACCGCC TGGACTTACC TCCATACGAC
    TCGTTTGAGG ATTTACGGGA GAAGCTTCTC ATGGCCGTGG AGAACGCTCA GGGATTCGAA
    GGCGTCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26569
    Clone ID Related Accession (Same CDS sequence) XM_012959652.2
    Accession Version XM_012959652.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGGCGCAA AAGACTTCCC CGTGCGCAGA
    GCAGTGAAGG ACACCCTGTC CAACCCACAG TCTCCACAGC CGTCACCTTA TAATTCTCCA
    AAGCCACAAC ACAAAGGGGC CCAGAGCTTC TTGCCGCCGG GCTGGGAGAT GAGAATAGCA
    CCCAATGGCA GACCATTCTT TATTGACCAT AACACAAAAA CTACCACATG GGAGGATCCG
    CGACTGAAGT TCCCAGTACA CATGAGAACA AAAGCTTCCT TGAACCCTAA TGATCTGGGT
    CCTCTCCCTC CGGGCTGGGA GGAAAGAATC CACGGGGATG GGCGAACGTT CTATATCGAT
    CACAGAAGTC CAGGGTTGAG GTGTCGAGAC ACAGGTACCA GCTGCTCGGT GCCCCCCTAC
    GACACCAAAA TAACCCAATG GGAGGACCCC CGGCTTCAGA ATCCCGCCAT CACTGGCCCT
    GCCGTCCCCT ACTCAAGAGA GTTTAAGCAG AAATACGACT ACTTCAGGAA GAAGTTGAAG
    AAACCTGCGG ATATTCCAAA TCGATTTGAG ATGAAGCTTC ACAGAAACAA CATTTTCGAG
    GAATCCTATA GGAGGATAAT GTCGGTGAAG CGGCCGGATG TCCTTAAAGC CAGATTATGG
    ATTGAATTTG AGTCGGAAAA AGGCCTCGAT TATGGAGGAG TAGCAAGGGA ATGGTTCTTC
    CTTCTGTCCA AAGAGATGTT CAACCCATAC TACGGGCTGT TTGAGTACTC CGCTACGGAC
    AATTACACAC TTCAGATAAA TCCCAATTCG GGTCTTTGCA ATGAAGATCA CCTTTCGTAC
    TTCACGTTTA TTGGTCGGAT TGCTGGGCTC GCCGTGTTCC ATGGAAAATT ATTAGATGGT
    TTCTTCATTA GACCATTCTA TAAGATGATG CTGGGGAAAC AAATAACGCT GAAAGACATG
    GAGTCAGTGG ACAGTGAATA CTACAATTCC TTAAAATGGA TCTTAGAAAA CGACCCAACC
    GAGCTGGATC TCAGGTTTTG CATAGATGAA GAAAACTTCG GTCAGACGTA TCAGGTGGAT
    CTGAAGCCCA ATGGATCTGA GATGGTCGTG ACCAATGACA ACAAGCGAGA GTACATTGAC
    CTCGTGATCC AATGGAGGTT TGTCAACCGG GTACAGAAGC AGATGAATGC CTTTTTAGAA
    GGTTTTACTG AACTCATTCC TATTGATCTA ATAAAAATCT TTGATGAAAA TGAGCTGGAG
    TTGCTAATGT GCGGCCTTGG TGATGTCGAT GTGAATGACT GGAGGCAACA CACCCTGTAT
    AAGAACGGCT ACTGCCCCAA CCACCCCGCC ATACAGTGGT TCTGGAAGGC GGTGTTATTA
    ATGGATGCAG AGAAACGCAT ACGCTTGCTG CAGTTTGTGA CTGGGACGTC ACGTGTGCCT
    ATGAATGGAT TTGCTGAACT GTACGGTTCA AATGGTCCTC AGCTGTTTAC AATAGAGCAG
    TGGGGAAGCC CCGACAAGCT GCCCAGAGCT CACACATGCT TTAACCGCCT GGACTTACCT
    CCATACGACT CGTTTGAGGA TTTACGGGAG AAGCTTCTCA TGGCCGTGGA GAACGCTCAG
    GGATTCGAAG GCGTCGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815106.1
    CDS609..4169
    Translation

    Target ORF information:

    RefSeq Version XM_012959652.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959652.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAAACA
    GCTTGGGAGA GGCAAAAGCA GAGAGAACCG TCCACGAGAT TAAGGTCTTG CAGTGTAACA
    GATGGTGTCG CCGAGCAGGC TCATTTATCA CTGGGCGCAA AAGACTTCCC CGTGCGCAGA
    GCAGTGAAGG ACACCCTGTC CAACCCACAG TCTCCACAGC CGTCACCTTA TAATTCTCCA
    AAGCCACAAC ACAAAGGGGC CCAGAGCTTC TTGCCGCCGG GCTGGGAGAT GAGAATAGCA
    CCCAATGGCA GACCATTCTT TATTGACCAT AACACAAAAA CTACCACATG GGAGGATCCG
    CGACTGAAGT TCCCAGTACA CATGAGAACA AAAGCTTCCT TGAACCCTAA TGATCTGGGT
    CCTCTCCCTC CGGGCTGGGA GGAAAGAATC CACGGGGATG GGCGAACGTT CTATATCGAT
    CACAGAAGTC CAGGGTTGAG GTGTCGAGAC ACAGGTACCA GCTGCTCGGT GCCCCCCTAC
    GACACCAAAA TAACCCAATG GGAGGACCCC CGGCTTCAGA ATCCCGCCAT CACTGGCCCT
    GCCGTCCCCT ACTCAAGAGA GTTTAAGCAG AAATACGACT ACTTCAGGAA GAAGTTGAAG
    AAACCTGCGG ATATTCCAAA TCGATTTGAG ATGAAGCTTC ACAGAAACAA CATTTTCGAG
    GAATCCTATA GGAGGATAAT GTCGGTGAAG CGGCCGGATG TCCTTAAAGC CAGATTATGG
    ATTGAATTTG AGTCGGAAAA AGGCCTCGAT TATGGAGGAG TAGCAAGGGA ATGGTTCTTC
    CTTCTGTCCA AAGAGATGTT CAACCCATAC TACGGGCTGT TTGAGTACTC CGCTACGGAC
    AATTACACAC TTCAGATAAA TCCCAATTCG GGTCTTTGCA ATGAAGATCA CCTTTCGTAC
    TTCACGTTTA TTGGTCGGAT TGCTGGGCTC GCCGTGTTCC ATGGAAAATT ATTAGATGGT
    TTCTTCATTA GACCATTCTA TAAGATGATG CTGGGGAAAC AAATAACGCT GAAAGACATG
    GAGTCAGTGG ACAGTGAATA CTACAATTCC TTAAAATGGA TCTTAGAAAA CGACCCAACC
    GAGCTGGATC TCAGGTTTTG CATAGATGAA GAAAACTTCG GTCAGACGTA TCAGGTGGAT
    CTGAAGCCCA ATGGATCTGA GATGGTCGTG ACCAATGACA ACAAGCGAGA GTACATTGAC
    CTCGTGATCC AATGGAGGTT TGTCAACCGG GTACAGAAGC AGATGAATGC CTTTTTAGAA
    GGTTTTACTG AACTCATTCC TATTGATCTA ATAAAAATCT TTGATGAAAA TGAGCTGGAG
    TTGCTAATGT GCGGCCTTGG TGATGTCGAT GTGAATGACT GGAGGCAACA CACCCTGTAT
    AAGAACGGCT ACTGCCCCAA CCACCCCGCC ATACAGTGGT TCTGGAAGGC GGTGTTATTA
    ATGGATGCAG AGAAACGCAT ACGCTTGCTG CAGTTTGTGA CTGGGACGTC ACGTGTGCCT
    ATGAATGGAT TTGCTGAACT GTACGGTTCA AATGGTCCTC AGCTGTTTAC AATAGAGCAG
    TGGGGAAGCC CCGACAAGCT GCCCAGAGCT CACACATGCT TTAACCGCCT GGACTTACCT
    CCATACGACT CGTTTGAGGA TTTACGGGAG AAGCTTCTCA TGGCCGTGGA GAACGCTCAG
    GGATTCGAAG GCGTCGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26570
    Clone ID Related Accession (Same CDS sequence) XM_012959653.2
    Accession Version XM_012959653.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTCGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815107.1
    CDS609..4145
    Translation

    Target ORF information:

    RefSeq Version XM_012959653.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959653.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTCAGAGA
    GAACCGTCCA CGAGATTAAG GTCTTGCAGT GTAACAGATG GTGTCGCCGA GCAGGCTCAT
    TTATCACTGG GCGCAAAAGA CTTCCCCGTG CGCAGAGCAG TGAAGGACAC CCTGTCCAAC
    CCACAGTCTC CACAGCCGTC ACCTTATAAT TCTCCAAAGC CACAACACAA AGGGGCCCAG
    AGCTTCTTGC CGCCGGGCTG GGAGATGAGA ATAGCACCCA ATGGCAGACC ATTCTTTATT
    GACCATAACA CAAAAACTAC CACATGGGAG GATCCGCGAC TGAAGTTCCC AGTACACATG
    AGAACAAAAG CTTCCTTGAA CCCTAATGAT CTGGGTCCTC TCCCTCCGGG CTGGGAGGAA
    AGAATCCACG GGGATGGGCG AACGTTCTAT ATCGATCACA GAAGTCCAGG GTTGAGGTGT
    CGAGACACAG GTACCAGCTG CTCGGTGCCC CCCTACGACA CCAAAATAAC CCAATGGGAG
    GACCCCCGGC TTCAGAATCC CGCCATCACT GGCCCTGCCG TCCCCTACTC AAGAGAGTTT
    AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTGAAGAAAC CTGCGGATAT TCCAAATCGA
    TTTGAGATGA AGCTTCACAG AAACAACATT TTCGAGGAAT CCTATAGGAG GATAATGTCG
    GTGAAGCGGC CGGATGTCCT TAAAGCCAGA TTATGGATTG AATTTGAGTC GGAAAAAGGC
    CTCGATTATG GAGGAGTAGC AAGGGAATGG TTCTTCCTTC TGTCCAAAGA GATGTTCAAC
    CCATACTACG GGCTGTTTGA GTACTCCGCT ACGGACAATT ACACACTTCA GATAAATCCC
    AATTCGGGTC TTTGCAATGA AGATCACCTT TCGTACTTCA CGTTTATTGG TCGGATTGCT
    GGGCTCGCCG TGTTCCATGG AAAATTATTA GATGGTTTCT TCATTAGACC ATTCTATAAG
    ATGATGCTGG GGAAACAAAT AACGCTGAAA GACATGGAGT CAGTGGACAG TGAATACTAC
    AATTCCTTAA AATGGATCTT AGAAAACGAC CCAACCGAGC TGGATCTCAG GTTTTGCATA
    GATGAAGAAA ACTTCGGTCA GACGTATCAG GTGGATCTGA AGCCCAATGG ATCTGAGATG
    GTCGTGACCA ATGACAACAA GCGAGAGTAC ATTGACCTCG TGATCCAATG GAGGTTTGTC
    AACCGGGTAC AGAAGCAGAT GAATGCCTTT TTAGAAGGTT TTACTGAACT CATTCCTATT
    GATCTAATAA AAATCTTTGA TGAAAATGAG CTGGAGTTGC TAATGTGCGG CCTTGGTGAT
    GTCGATGTGA ATGACTGGAG GCAACACACC CTGTATAAGA ACGGCTACTG CCCCAACCAC
    CCCGCCATAC AGTGGTTCTG GAAGGCGGTG TTATTAATGG ATGCAGAGAA ACGCATACGC
    TTGCTGCAGT TTGTGACTGG GACGTCACGT GTGCCTATGA ATGGATTTGC TGAACTGTAC
    GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAGTGGG GAAGCCCCGA CAAGCTGCCC
    AGAGCTCACA CATGCTTTAA CCGCCTGGAC TTACCTCCAT ACGACTCGTT TGAGGATTTA
    CGGGAGAAGC TTCTCATGGC CGTGGAGAAC GCTCAGGGAT TCGAAGGCGT CGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26571
    Clone ID Related Accession (Same CDS sequence) XM_012959655.2
    Accession Version XM_012959655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTC
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815109.1
    CDS609..4142
    Translation

    Target ORF information:

    RefSeq Version XM_012959655.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCACC GGCTACGGTT TCATTTTGGC TCCGGGCGAA GCAACACGGC TCCCGAGTCG 
    GATGTCCTGG AGCAGGAGAA GGAGGACGAC TTCTTCATGG CATTCCACAC GCTGCCGAGG
    AGGAACGGCT CTCACCCTTT CTCCCGGCAC GGATCGGAGT GTGGAGAGAG CGAGCTCCCC
    GAAGAGGGGG CACTAAAAAG GAGCACGTCC ATGTTTATCC CCCAACTTCT CAACAACATT
    GACATACGCC CGACGAGAAG CTCCTCCGTG CAGATCTCGC TGCAGCGCAA CTCGGCAAAC
    GGGCACGTCG ATGAGTTCGA GGCGCCGGAA TATCCAGACG AGGCACTGGC ATGTGATTTT
    GGGGACTTTA ATGAACATTA CATTTCAGCC GTCAGGAAAA ATTCCTCTTC TGCCAGCAGC
    CCCCTAGATA CCCCTGGAAA TTCTCCTCCT CAGAGACGGG ATACGACAAG GCAGCCTATA
    AATGGCGCTG CCCCATTCTT ACCTCGGCTT ACAGTGCGCC CCATTGTGGA ACAGAACGGC
    CATATTAACT GTGCCGCCGA TATGCAAGAA GAGGTGAGCA TAACCACAAG CGGCCAGAAC
    ACAAGTGGGA TAAGAATAAA GCGCCAGCCT TTAAGCCCTG ATATACGGCA GGTTAGGCTG
    CTGTCTCCGT GTCAGGAAAA TCAGAACGGG TCTAATACTG AACCCAGGCG CTGGTCTCTA
    CAGCATCTTC CTGACACCTC AGGGAGCCCT GGGAAAAGAT GCTTCGTGTT CCAACTGCAG
    CAGTCTCAAA CCGGGAACTC CAATATGGGG GGAGAGTATA ACTTTGGCTT CACCGGCACG
    AAAGGCGACA GGTTGGTCCG ATATCCTCGT ATTAGGCTGG AAAGAAGTAC TTCATACCCG
    CTCCAGCCGC GGCCTGAAAG CACCAGCCCA GTGGAAAATG GATTAAGCTC TGAATCCGGC
    AAGTGCAAGA AAGACTACGC TGAGATTCTG CCCAACGATT CCAGGGAGAG ACCCTCACAG
    GGGCAGGGGT GCACCTTCAA AATACGCCAG GAGTCAAATG TTCGGCAGCC ACATTTCAGG
    ATCCGCGTCA CTCGCAGCAG TGAGGAGCAA GGTACAGAAG AAGGGCAAGA TGTGCCTAGA
    AATTCAGCTT TGGGAAAGGC CACGACAAGA GATGATTTTT TGGGCCAAGT TGATGTTCCG
    CTGAATCACT TACCGACAGA AGATCCAACA ATGGAAAGGC CATACACATT CAAAGACTTC
    TTACTCAGAC CAAGAAGCCA CAAATCCCGG GTTAAGGGTT TCTTGAGACT GAAGATGGCC
    TACTTGCCAA AGAATGGAAT GCAGGAAGAG GAGAATAATG AGCAAAGAGA AGAATCCGAG
    CAGGCCTGGG ATGTGGTGGA CTCCAATGAT TCAAGCTCTC CACATCAGCA AGAGCTCCCA
    GCTCCACCTA TGCCGCCGGG ATGGGAAGAA AAAGTGGACA ACCTTGGGCG GACATATTAC
    GTCAACCATA ATAACAAGAC CACTCAGTGG CAAAGGCCAA GCCTTGTAGA CGTTACGTCC
    GAATCGGACA GCAACATTCG GCACATACAG CAAGAGGCCC ACCGAGTGTT CCGATCTCGG
    AGACATATCA GTGAAGACCT AGAACCCGAC CATATAGAAG GAGGAGCGGA TGTTCCAGAG
    CCATGGGAAA CCATTTCCGA GGAAATGACT TTGACGACCG ACACGTTAAA CCAGTCTCTC
    CCACCCCCTG CTTCCCCGGA TTCTCGGGCC AGCGCTCTGG AACTGTCCGA GGAGCTGAAC
    AGGAGGCTGC AGATTAGCTC CGAGTCCAAC GGAGAACAGT TCAGTTCTAT CATTAGAGAA
    CCGTCCACGA GATTAAGGTC TTGCAGTGTA ACAGATGGTG TCGCCGAGCA GGCTCATTTA
    TCACTGGGCG CAAAAGACTT CCCCGTGCGC AGAGCAGTGA AGGACACCCT GTCCAACCCA
    CAGTCTCCAC AGCCGTCACC TTATAATTCT CCAAAGCCAC AACACAAAGG GGCCCAGAGC
    TTCTTGCCGC CGGGCTGGGA GATGAGAATA GCACCCAATG GCAGACCATT CTTTATTGAC
    CATAACACAA AAACTACCAC ATGGGAGGAT CCGCGACTGA AGTTCCCAGT ACACATGAGA
    ACAAAAGCTT CCTTGAACCC TAATGATCTG GGTCCTCTCC CTCCGGGCTG GGAGGAAAGA
    ATCCACGGGG ATGGGCGAAC GTTCTATATC GATCACAGAA GTCCAGGGTT GAGGTGTCGA
    GACACAGGTA CCAGCTGCTC GGTGCCCCCC TACGACACCA AAATAACCCA ATGGGAGGAC
    CCCCGGCTTC AGAATCCCGC CATCACTGGC CCTGCCGTCC CCTACTCAAG AGAGTTTAAG
    CAGAAATACG ACTACTTCAG GAAGAAGTTG AAGAAACCTG CGGATATTCC AAATCGATTT
    GAGATGAAGC TTCACAGAAA CAACATTTTC GAGGAATCCT ATAGGAGGAT AATGTCGGTG
    AAGCGGCCGG ATGTCCTTAA AGCCAGATTA TGGATTGAAT TTGAGTCGGA AAAAGGCCTC
    GATTATGGAG GAGTAGCAAG GGAATGGTTC TTCCTTCTGT CCAAAGAGAT GTTCAACCCA
    TACTACGGGC TGTTTGAGTA CTCCGCTACG GACAATTACA CACTTCAGAT AAATCCCAAT
    TCGGGTCTTT GCAATGAAGA TCACCTTTCG TACTTCACGT TTATTGGTCG GATTGCTGGG
    CTCGCCGTGT TCCATGGAAA ATTATTAGAT GGTTTCTTCA TTAGACCATT CTATAAGATG
    ATGCTGGGGA AACAAATAAC GCTGAAAGAC ATGGAGTCAG TGGACAGTGA ATACTACAAT
    TCCTTAAAAT GGATCTTAGA AAACGACCCA ACCGAGCTGG ATCTCAGGTT TTGCATAGAT
    GAAGAAAACT TCGGTCAGAC GTATCAGGTG GATCTGAAGC CCAATGGATC TGAGATGGTC
    GTGACCAATG ACAACAAGCG AGAGTACATT GACCTCGTGA TCCAATGGAG GTTTGTCAAC
    CGGGTACAGA AGCAGATGAA TGCCTTTTTA GAAGGTTTTA CTGAACTCAT TCCTATTGAT
    CTAATAAAAA TCTTTGATGA AAATGAGCTG GAGTTGCTAA TGTGCGGCCT TGGTGATGTC
    GATGTGAATG ACTGGAGGCA ACACACCCTG TATAAGAACG GCTACTGCCC CAACCACCCC
    GCCATACAGT GGTTCTGGAA GGCGGTGTTA TTAATGGATG CAGAGAAACG CATACGCTTG
    CTGCAGTTTG TGACTGGGAC GTCACGTGTG CCTATGAATG GATTTGCTGA ACTGTACGGT
    TCAAATGGTC CTCAGCTGTT TACAATAGAG CAGTGGGGAA GCCCCGACAA GCTGCCCAGA
    GCTCACACAT GCTTTAACCG CCTGGACTTA CCTCCATACG ACTCGTTTGA GGATTTACGG
    GAGAAGCTTC TCATGGCCGT GGAGAACGCT CAGGGATTCG AAGGCGTCGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26572
    Clone ID Related Accession (Same CDS sequence) XM_012959661.2
    Accession Version XM_012959661.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAAACAGCTT GGGAGAGGCA AAAGCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTATCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTCGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815115.1
    CDS20..3058
    Translation

    Target ORF information:

    RefSeq Version XM_012959661.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959661.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAAACAGCTT GGGAGAGGCA AAAGCAGAGA GAACCGTCCA CGAGATTAAG GTCTTGCAGT
    GTAACAGATG GTGTCGCCGA GCAGGCTCAT TTATCACTGC CGAGCGTCTC AACGGGAAGA
    GCTCGTTCCT CAACTGTCAC AGGCGGTGAG GATTCTACGC CAACCGTGGC CTATGTGCAC
    ACCACTCCGG GTTTGCCTTC AGGATGGGAG GAGAGGAAAG ACGCTAAAGG CCGTACCTAT
    TATGTCAATC ATAACAATCG CACCACCACA TGGACCAGAC CTATTGTGCA GCACGCCGAG
    GACGGGGCAG TGGGATCTAC TTCAAACAGT AGTAACCATC TGAGCGAGCC TCAGATCCGA
    CGGCCTCGTA GTCTCAGTTC TCCCACAGTT ACCCTATCTG CACCACTCGA GGGCGCAAAA
    GACTTCCCCG TGCGCAGAGC AGTGAAGGAC ACCCTGTCCA ACCCACAGTC TCCACAGCCG
    TCACCTTATA ATTCTCCAAA GCCACAACAC AAAGGGGCCC AGAGCTTCTT GCCGCCGGGC
    TGGGAGATGA GAATAGCACC CAATGGCAGA CCATTCTTTA TTGACCATAA CACAAAAACT
    ACCACATGGG AGGATCCGCG ACTGAAGTTC CCAGTACACA TGAGAACAAA AGCTTCCTTG
    AACCCTAATG ATCTGGGTCC TCTCCCTCCG GGCTGGGAGG AAAGAATCCA CGGGGATGGG
    CGAACGTTCT ATATCGATCA CAGAAGTCCA GGGTTGAGGT GTCGAGACAC AGGTACCAGC
    TGCTCGGTGC CCCCCTACGA CACCAAAATA ACCCAATGGG AGGACCCCCG GCTTCAGAAT
    CCCGCCATCA CTGGCCCTGC CGTCCCCTAC TCAAGAGAGT TTAAGCAGAA ATACGACTAC
    TTCAGGAAGA AGTTGAAGAA ACCTGCGGAT ATTCCAAATC GATTTGAGAT GAAGCTTCAC
    AGAAACAACA TTTTCGAGGA ATCCTATAGG AGGATAATGT CGGTGAAGCG GCCGGATGTC
    CTTAAAGCCA GATTATGGAT TGAATTTGAG TCGGAAAAAG GCCTCGATTA TGGAGGAGTA
    GCAAGGGAAT GGTTCTTCCT TCTGTCCAAA GAGATGTTCA ACCCATACTA CGGGCTGTTT
    GAGTACTCCG CTACGGACAA TTACACACTT CAGATAAATC CCAATTCGGG TCTTTGCAAT
    GAAGATCACC TTTCGTACTT CACGTTTATT GGTCGGATTG CTGGGCTCGC CGTGTTCCAT
    GGAAAATTAT TAGATGGTTT CTTCATTAGA CCATTCTATA AGATGATGCT GGGGAAACAA
    ATAACGCTGA AAGACATGGA GTCAGTGGAC AGTGAATACT ACAATTCCTT AAAATGGATC
    TTAGAAAACG ACCCAACCGA GCTGGATCTC AGGTTTTGCA TAGATGAAGA AAACTTCGGT
    CAGACGTATC AGGTGGATCT GAAGCCCAAT GGATCTGAGA TGGTCGTGAC CAATGACAAC
    AAGCGAGAGT ACATTGACCT CGTGATCCAA TGGAGGTTTG TCAACCGGGT ACAGAAGCAG
    ATGAATGCCT TTTTAGAAGG TTTTACTGAA CTCATTCCTA TTGATCTAAT AAAAATCTTT
    GATGAAAATG AGCTGGAGTT GCTAATGTGC GGCCTTGGTG ATGTCGATGT GAATGACTGG
    AGGCAACACA CCCTGTATAA GAACGGCTAC TGCCCCAACC ACCCCGCCAT ACAGTGGTTC
    TGGAAGGCGG TGTTATTAAT GGATGCAGAG AAACGCATAC GCTTGCTGCA GTTTGTGACT
    GGGACGTCAC GTGTGCCTAT GAATGGATTT GCTGAACTGT ACGGTTCAAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAGTG GGGAAGCCCC GACAAGCTGC CCAGAGCTCA CACATGCTTT
    AACCGCCTGG ACTTACCTCC ATACGACTCG TTTGAGGATT TACGGGAGAA GCTTCTCATG
    GCCGTGGAGA ACGCTCAGGG ATTCGAAGGC GTCGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26573
    Clone ID Related Accession (Same CDS sequence) XM_012959663.2
    Accession Version XM_012959663.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGGCCT GGGATGTGGT GGACTCCAAT GATTCAAGCT CTCCACATCA GCAAGAGCTC
    CCAGCTCCAC CTATGCCGCC GGGATGGGAA GAAAAAGTGG ACAACCTTGG GCGGACATAT
    TACGTCAACC ATAATAACAA GACCACTCAG TGGCAAAGGC CAAGCCTTGT AGACGTTACG
    TCCGAATCGG ACAGCAACAT TCGGCACATA CAGCAAGAGG CCCACCGAGT GTTCCGATCT
    CGGAGACATA TCAGTGAAGA CCTAGAACCC GACCATATAG AAGGAGGAGC GGATGTTCCA
    GAGCCATGGG AAACCATTTC CGAGGAAATG ACTTTGACGA CCGACACGTT AAACCAGTCT
    CTCCCACCCC CTGCTTCCCC GGATTCTCGG GCCAGCGCTC TGGAACTGTC CGAGGAGCTG
    AACAGGAGGC TGCAGATTAG CTCCGAGTCC AACGGAGAAC AGTTCAGTTC TATCATTCAA
    ACAGCTTGGG AGAGGCAAAA GCAGAGAGAA CCGTCCACGA GATTAAGGTC TTGCAGTGTA
    ACAGATGGTG TCGCCGAGCA GGCTCATTTA TCACTGCCGA GCGTCTCAAC GGGAAGAGCT
    CGTTCCTCAA CTGTCACAGG CGGTGAGGAT TCTACGCCAA CCGTGGCCTA TGTGCACACC
    ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG AGGAAAGACG CTAAAGGCCG TACCTATTAT
    GTCAATCATA ACAATCGCAC CACCACATGG ACCAGACCTA TTGTGCAGCA CGCCGAGGAC
    GGGGCAGTGG GATCTACTTC AAACAGTAGT AACCATCTGA GCGAGCCTCA GATCCGACGG
    CCTCGTAGTC TCAGTTCTCC CACAGTTACC CTATCTGCAC CACTCGAGGG CGCAAAAGAC
    TTCCCCGTGC GCAGAGCAGT GAAGGACACC CTGTCCAACC CACAGTCTCC ACAGCCGTCA
    CCTTATAATT CTCCAAAGCC ACAACACAAA GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG
    GAGATGAGAA TAGCACCCAA TGGCAGACCA TTCTTTATTG ACCATAACAC AAAAACTACC
    ACATGGGAGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAG AAGTCCAGGG TTGAGGTGTC GAGACACAGG TACCAGCTGC
    TCGGTGCCCC CCTACGACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815117.1
    CDS20..3055
    Translation

    Target ORF information:

    RefSeq Version XM_012959663.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGGCCT GGGATGTGGT GGACTCCAAT GATTCAAGCT CTCCACATCA GCAAGAGCTC
    CCAGCTCCAC CTATGCCGCC GGGATGGGAA GAAAAAGTGG ACAACCTTGG GCGGACATAT
    TACGTCAACC ATAATAACAA GACCACTCAG TGGCAAAGGC CAAGCCTTGT AGACGTTACG
    TCCGAATCGG ACAGCAACAT TCGGCACATA CAGCAAGAGG CCCACCGAGT GTTCCGATCT
    CGGAGACATA TCAGTGAAGA CCTAGAACCC GACCATATAG AAGGAGGAGC GGATGTTCCA
    GAGCCATGGG AAACCATTTC CGAGGAAATG ACTTTGACGA CCGACACGTT AAACCAGTCT
    CTCCCACCCC CTGCTTCCCC GGATTCTCGG GCCAGCGCTC TGGAACTGTC CGAGGAGCTG
    AACAGGAGGC TGCAGATTAG CTCCGAGTCC AACGGAGAAC AGTTCAGTTC TATCATTCAA
    ACAGCTTGGG AGAGGCAAAA GCAGAGAGAA CCGTCCACGA GATTAAGGTC TTGCAGTGTA
    ACAGATGGTG TCGCCGAGCA GGCTCATTTA TCACTGCCGA GCGTCTCAAC GGGAAGAGCT
    CGTTCCTCAA CTGTCACAGG CGGTGAGGAT TCTACGCCAA CCGTGGCCTA TGTGCACACC
    ACTCCGGGTT TGCCTTCAGG ATGGGAGGAG AGGAAAGACG CTAAAGGCCG TACCTATTAT
    GTCAATCATA ACAATCGCAC CACCACATGG ACCAGACCTA TTGTGCAGCA CGCCGAGGAC
    GGGGCAGTGG GATCTACTTC AAACAGTAGT AACCATCTGA GCGAGCCTCA GATCCGACGG
    CCTCGTAGTC TCAGTTCTCC CACAGTTACC CTATCTGCAC CACTCGAGGG CGCAAAAGAC
    TTCCCCGTGC GCAGAGCAGT GAAGGACACC CTGTCCAACC CACAGTCTCC ACAGCCGTCA
    CCTTATAATT CTCCAAAGCC ACAACACAAA GGGGCCCAGA GCTTCTTGCC GCCGGGCTGG
    GAGATGAGAA TAGCACCCAA TGGCAGACCA TTCTTTATTG ACCATAACAC AAAAACTACC
    ACATGGGAGG ATCCGCGACT GAAGTTCCCA GTACACATGA GAACAAAAGC TTCCTTGAAC
    CCTAATGATC TGGGTCCTCT CCCTCCGGGC TGGGAGGAAA GAATCCACGG GGATGGGCGA
    ACGTTCTATA TCGATCACAG AAGTCCAGGG TTGAGGTGTC GAGACACAGG TACCAGCTGC
    TCGGTGCCCC CCTACGACAC CAAAATAACC CAATGGGAGG ACCCCCGGCT TCAGAATCCC
    GCCATCACTG GCCCTGCCGT CCCCTACTCA AGAGAGTTTA AGCAGAAATA CGACTACTTC
    AGGAAGAAGT TGAAGAAACC TGCGGATATT CCAAATCGAT TTGAGATGAA GCTTCACAGA
    AACAACATTT TCGAGGAATC CTATAGGAGG ATAATGTCGG TGAAGCGGCC GGATGTCCTT
    AAAGCCAGAT TATGGATTGA ATTTGAGTCG GAAAAAGGCC TCGATTATGG AGGAGTAGCA
    AGGGAATGGT TCTTCCTTCT GTCCAAAGAG ATGTTCAACC CATACTACGG GCTGTTTGAG
    TACTCCGCTA CGGACAATTA CACACTTCAG ATAAATCCCA ATTCGGGTCT TTGCAATGAA
    GATCACCTTT CGTACTTCAC GTTTATTGGT CGGATTGCTG GGCTCGCCGT GTTCCATGGA
    AAATTATTAG ATGGTTTCTT CATTAGACCA TTCTATAAGA TGATGCTGGG GAAACAAATA
    ACGCTGAAAG ACATGGAGTC AGTGGACAGT GAATACTACA ATTCCTTAAA ATGGATCTTA
    GAAAACGACC CAACCGAGCT GGATCTCAGG TTTTGCATAG ATGAAGAAAA CTTCGGTCAG
    ACGTATCAGG TGGATCTGAA GCCCAATGGA TCTGAGATGG TCGTGACCAA TGACAACAAG
    CGAGAGTACA TTGACCTCGT GATCCAATGG AGGTTTGTCA ACCGGGTACA GAAGCAGATG
    AATGCCTTTT TAGAAGGTTT TACTGAACTC ATTCCTATTG ATCTAATAAA AATCTTTGAT
    GAAAATGAGC TGGAGTTGCT AATGTGCGGC CTTGGTGATG TCGATGTGAA TGACTGGAGG
    CAACACACCC TGTATAAGAA CGGCTACTGC CCCAACCACC CCGCCATACA GTGGTTCTGG
    AAGGCGGTGT TATTAATGGA TGCAGAGAAA CGCATACGCT TGCTGCAGTT TGTGACTGGG
    ACGTCACGTG TGCCTATGAA TGGATTTGCT GAACTGTACG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAGTGGGG AAGCCCCGAC AAGCTGCCCA GAGCTCACAC ATGCTTTAAC
    CGCCTGGACT TACCTCCATA CGACTCGTTT GAGGATTTAC GGGAGAAGCT TCTCATGGCC
    GTGGAGAACG CTCAGGGATT CGAAGGCGTC GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26574
    Clone ID Related Accession (Same CDS sequence) XM_012959667.2
    Accession Version XM_012959667.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815121.1
    CDS19..3033
    Translation

    Target ORF information:

    RefSeq Version XM_012959667.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (nedd4l), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959667.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAAAC TGCCCCATCA GGAAATCTGT AGGATTATAG AGAAATGGGC AGTCTGCCCC 
    AGCGTGCCTC GGGAGTGCAA GGAGGAGTCG CGCATCCTCA GAGTAAAAAT TGTTTCCGGC
    ACTGACTTGG CAAAGAAGGA TATATTTGGG GCCAGTGATC CCTATGTGAA ACTGTCCCTG
    TATGTGGCGG ATGAAAACCG TGAGCTGGCT CTGGTACAGA CAAAGACAAT AAAGAAGACC
    TTGAACCCAA AATGGAATGA AGAATTTTTT TTCAGGGTTC ACCCGACCAA TCACAGGCTG
    CTGTTTGAAG TATTTGATGA GAACCGATTG ACAAGAGATG ATTTTTTGGG CCAAGTTGAT
    GTTCCGCTGA ATCACTTACC GACAGAAGAT CCAACAATGG AAAGGCCATA CACATTCAAA
    GACTTCTTAC TCAGACCAAG AAGCCACAAA TCCCGGGTTA AGGGTTTCTT GAGACTGAAG
    ATGGCCTACT TGCCAAAGAA TGGAATGCAG GAAGAGGAGA ATAATGAGCA AAGAGAAGAA
    TCCGAGCAGG CCTGGGATGT GGTGGACTCC AATGATTCAA GCTCTCCACA TCAGCAAGAG
    CTCCCAGCTC CACCTATGCC GCCGGGATGG GAAGAAAAAG TGGACAACCT TGGGCGGACA
    TATTACGTCA ACCATAATAA CAAGACCACT CAGTGGCAAA GGCCAAGCCT TGTAGACGTT
    ACGTCCGAAT CGGACAGCAA CATTCGGCAC ATACAGCAAG AGGCCCACCG AGTGTTCCGA
    TCTCGGAGAC ATATCAGTGA AGACCTAGAA CCCGACCATA TAGAAGGAGG AGCGGATGTT
    CCAGAGCCAT GGGAAACCAT TTCCGAGGAA ATGACTTTGA CGACCGACAC GTTAAACCAG
    TCTCTCCCAC CCCCTGCTTC CCCGGATTCT CGGGCCAGCG CTCTGGAACT GTCCGAGGAG
    CTGAACAGGA GGCTGCAGAT TAGCTCCGAG TCCAACGGAG AACAGTTCAG TTCTATCATT
    CAGAGAGAAC CGTCCACGAG ATTAAGGTCT TGCAGTGTAA CAGATGGTGT CGCCGAGCAG
    GCTCATTTAT CACTGCCGAG CGTCTCAACG GGAAGAGCTC GTTCCTCAAC TGTCACAGGC
    GGTGAGGATT CTACGCCAAC CGTGGCCTAT GTGCACACCA CTCCGGGTTT GCCTTCAGGA
    TGGGAGGAGA GGAAAGACGC TAAAGGCCGT ACCTATTATG TCAATCATAA CAATCGCACC
    ACCACATGGA CCAGACCTAT TGTGCAGCAC GCCGAGGACG GGGCAGTGGG ATCTACTTCA
    AACAGTAGTA ACCATCTGAG CGAGCCTCAG ATCCGACGGC CTCGTAGTCT CAGTTCTCCC
    ACAGTTACCC TATCTGCACC ACTCGAGGGC GCAAAAGACT TCCCCGTGCG CAGAGCAGTG
    AAGGACACCC TGTCCAACCC ACAGTCTCCA CAGCCGTCAC CTTATAATTC TCCAAAGCCA
    CAACACAAAG GGGCCCAGAG CTTCTTGCCG CCGGGCTGGG AGATGAGAAT AGCACCCAAT
    GGCAGACCAT TCTTTATTGA CCATAACACA AAAACTACCA CATGGGAGGA TCCGCGACTG
    AAGTTCCCAG TACACATGAG AACAAAAGCT TCCTTGAACC CTAATGATCT GGGTCCTCTC
    CCTCCGGGCT GGGAGGAAAG AATCCACGGG GATGGGCGAA CGTTCTATAT CGATCACAGA
    AGTCCAGGGT TGAGGTGTCG AGACACAGGT ACCAGCTGCT CGGTGCCCCC CTACGACACC
    AAAATAACCC AATGGGAGGA CCCCCGGCTT CAGAATCCCG CCATCACTGG CCCTGCCGTC
    CCCTACTCAA GAGAGTTTAA GCAGAAATAC GACTACTTCA GGAAGAAGTT GAAGAAACCT
    GCGGATATTC CAAATCGATT TGAGATGAAG CTTCACAGAA ACAACATTTT CGAGGAATCC
    TATAGGAGGA TAATGTCGGT GAAGCGGCCG GATGTCCTTA AAGCCAGATT ATGGATTGAA
    TTTGAGTCGG AAAAAGGCCT CGATTATGGA GGAGTAGCAA GGGAATGGTT CTTCCTTCTG
    TCCAAAGAGA TGTTCAACCC ATACTACGGG CTGTTTGAGT ACTCCGCTAC GGACAATTAC
    ACACTTCAGA TAAATCCCAA TTCGGGTCTT TGCAATGAAG ATCACCTTTC GTACTTCACG
    TTTATTGGTC GGATTGCTGG GCTCGCCGTG TTCCATGGAA AATTATTAGA TGGTTTCTTC
    ATTAGACCAT TCTATAAGAT GATGCTGGGG AAACAAATAA CGCTGAAAGA CATGGAGTCA
    GTGGACAGTG AATACTACAA TTCCTTAAAA TGGATCTTAG AAAACGACCC AACCGAGCTG
    GATCTCAGGT TTTGCATAGA TGAAGAAAAC TTCGGTCAGA CGTATCAGGT GGATCTGAAG
    CCCAATGGAT CTGAGATGGT CGTGACCAAT GACAACAAGC GAGAGTACAT TGACCTCGTG
    ATCCAATGGA GGTTTGTCAA CCGGGTACAG AAGCAGATGA ATGCCTTTTT AGAAGGTTTT
    ACTGAACTCA TTCCTATTGA TCTAATAAAA ATCTTTGATG AAAATGAGCT GGAGTTGCTA
    ATGTGCGGCC TTGGTGATGT CGATGTGAAT GACTGGAGGC AACACACCCT GTATAAGAAC
    GGCTACTGCC CCAACCACCC CGCCATACAG TGGTTCTGGA AGGCGGTGTT ATTAATGGAT
    GCAGAGAAAC GCATACGCTT GCTGCAGTTT GTGACTGGGA CGTCACGTGT GCCTATGAAT
    GGATTTGCTG AACTGTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAGTGGGGA
    AGCCCCGACA AGCTGCCCAG AGCTCACACA TGCTTTAACC GCCTGGACTT ACCTCCATAC
    GACTCGTTTG AGGATTTACG GGAGAAGCTT CTCATGGCCG TGGAGAACGC TCAGGGATTC
    GAAGGCGTCG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.