eif5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following eif5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa05868 XM_002937859.5
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52480 XM_031897015.1
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OXa05868 XM_002937859.4 Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa05868
    Clone ID Related Accession (Same CDS sequence) XM_002937859.4 , XM_002937859.5
    Accession Version XM_002937859.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937859.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GCAACAGAAG AGGATGCCGG TGTGGATGAC
    TGGGAAGCTA TAGTGAGCGA TGAAGAAAGG GAGAAAGAGA CAAAAACTAT TCACATTGAA
    GTTCAGGAGA AGAATGAAGA GGAAGAAGAG GAGGACGAAG ATGAAGAGGA TGAGGGAGAA
    AGTGAGGAAG AGGATGAGGA TGAAGGGGAT AGTGAAGGCA CTGAAGATGG AAAGTCTTCA
    GATGAAAAGC CGCAAGAATC CCAAACTGTT GCACCAAAAC TTCAAGAGAA GAAGCCTAGC
    AGAGAACTAA GCTCCGAGTC TGAGTCTGAG TCTGATTCTG ATGACGATCG CACTAAAGAA
    GAGATAGCGT ACGACAAAGC AAAGAGGAGA ATAGAGAAAA GACGCCTTGA AAACCAGAAA
    ACTGTAAATA TCCAGGTACT CCGGGCACCA GTAATATGTG TCCTTGGGCA TGTAGACACA
    GGAAAAACAA AAATCTTGGA TAAGCTGCGC CATACACATG TACAAGACAG TGAAGCAGGT
    GGTATCACTC AACAGATTGG AGCAACCAAC GTCCCCCTTG ATGCTATCAA AGAACAGGCT
    AAGATGGTTA AGAATTTTGA CAGGGAGAAT ATAAAGATTC CAGGAATGCT TATCATAGAT
    ACACCTGGCC ATGAATCCTT CAGTAATTTG AGGAATCGTG GAAGCTCCCT TTGTGATATT
    GCCATTCTTG TTGTGGATAT TATGCATGGA TTGGAGCCGC AGACAATAGA ATCTCTGAAC
    CTGCTAAAAA ATAAGAAGTG CCCTTTTATT GTGGCTCTGA ACAAAATCGA CAGGCTGTAT
    GACTGGAAAA AGGGCCCAGA TGCTGATGTT GCTGCAACAT TAAAGAAACA GAAGAAAAAT
    ACTAAGGATG AATTTGAGGA GCGAGCCAAG GCCATCATTG TTGAATTTGC ACAGCAGGGT
    TTGAATGCTG CTTTATTCTA TGAAAATAAA GACCCTCGCA CTTTTGTTTC TCTGGTTCCT
    ACATCTGCTC ATACTGGAGA TGGAATGGGA AATCTGATCT CTCTTCTTGT AGACTTGACA
    CAGACCATGC TTAATAAAAG GCTGGCTCAT TCCGAAGAGT TAAGAGCCCA AGTAATGGAA
    GTGAAAGCCC TGCCAGGGAT GGGAACGACT ATTGATGTCA TTTTGATCAA TGGGCGTTTA
    AAGGAAAGTG ACATTGTTAT TGTTCCTGGT GTTGAAGGCC CAATAGTCAC CCAGATCAGA
    GGACTTTTGC TACCTCCCCC AATGAAAGAA TTAAGAGTTA AGAATCAGTA TGAGAAACAC
    AAAGAAGTAA TAGCTGCTCA AGGAGTTAAG ATTCTTGGGA AAGACTTGGA AAAGGCATTG
    GCTGGGTTGC CACTGTTAGT GGCCCACAGT GAGGATGAGA TCCCAGTTCT GAGGGATGAG
    CTCATCCATG AATTAAAACA GACCTTGAAT GCCATAAAGT TAGAAGAAAA GGGTGTTTAT
    GTGCAGGCAT CTACCCTGGG TTCTTTAGAA GCACTGCTAG AGTTCCTGAA AACATCAGAA
    GTACCGTATG CTGGAATCAA CATTGGACCT GTTCACAAAA AGGATGTCAT GAAAGCTTCA
    GTGATGTTGG AACATGATCC ACAATATGCT GTGATTTTGG CTTTTGATGT AAGGATTGAA
    CGAGAAGCCC AAGAAATGGC TGATAGTTTA GGAGTTCGCA TTTTTAGTGC TGAAATCATC
    TACCACTTAT TTGATGCCTT TACTAAGTAC CGCCAAGACT ATAAGAAGCA GAAACAGGAA
    GAATTCAAGC ACATAGCAGT ATTTCCGTGT AAAATCCGAA TTCTCCCTCA GTTTATCTTC
    AATTCTCGAG ATCCAATTGT CATGGGAGTG GTGGTGGAAG CAGGACAAGT GAAACAGGGA
    ACGCCAATTT GTGTACCTAG TAAGAATTTT GTAGATATTG GGATAGTGAC CAGTATTGAA
    ATTAACCACA AGTCAGTTGA TATGGCAAAG AAAGGCCAGG AAGTGTGCAT TAAAATCGAG
    CCCATTCCTG GAGAGTCTCC TAAGATGTTT GGAAGACACT TTGAGGCTAC AGATTTCCTC
    ATTAGCAAGA TTACTCGGCA GTCAATTGAT GCTTTGAAAA ACTGGTTTAG AGACGAGATG
    CAGAAATCAG ACTGGCAACT TATAGTGGAA CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937905.1
    CDS160..3756
    Translation

    Target ORF information:

    RefSeq Version XM_002937859.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937859.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GCAACAGAAG AGGATGCCGG TGTGGATGAC
    TGGGAAGCTA TAGTGAGCGA TGAAGAAAGG GAGAAAGAGA CAAAAACTAT TCACATTGAA
    GTTCAGGAGA AGAATGAAGA GGAAGAAGAG GAGGACGAAG ATGAAGAGGA TGAGGGAGAA
    AGTGAGGAAG AGGATGAGGA TGAAGGGGAT AGTGAAGGCA CTGAAGATGG AAAGTCTTCA
    GATGAAAAGC CGCAAGAATC CCAAACTGTT GCACCAAAAC TTCAAGAGAA GAAGCCTAGC
    AGAGAACTAA GCTCCGAGTC TGAGTCTGAG TCTGATTCTG ATGACGATCG CACTAAAGAA
    GAGATAGCGT ACGACAAAGC AAAGAGGAGA ATAGAGAAAA GACGCCTTGA AAACCAGAAA
    ACTGTAAATA TCCAGGTACT CCGGGCACCA GTAATATGTG TCCTTGGGCA TGTAGACACA
    GGAAAAACAA AAATCTTGGA TAAGCTGCGC CATACACATG TACAAGACAG TGAAGCAGGT
    GGTATCACTC AACAGATTGG AGCAACCAAC GTCCCCCTTG ATGCTATCAA AGAACAGGCT
    AAGATGGTTA AGAATTTTGA CAGGGAGAAT ATAAAGATTC CAGGAATGCT TATCATAGAT
    ACACCTGGCC ATGAATCCTT CAGTAATTTG AGGAATCGTG GAAGCTCCCT TTGTGATATT
    GCCATTCTTG TTGTGGATAT TATGCATGGA TTGGAGCCGC AGACAATAGA ATCTCTGAAC
    CTGCTAAAAA ATAAGAAGTG CCCTTTTATT GTGGCTCTGA ACAAAATCGA CAGGCTGTAT
    GACTGGAAAA AGGGCCCAGA TGCTGATGTT GCTGCAACAT TAAAGAAACA GAAGAAAAAT
    ACTAAGGATG AATTTGAGGA GCGAGCCAAG GCCATCATTG TTGAATTTGC ACAGCAGGGT
    TTGAATGCTG CTTTATTCTA TGAAAATAAA GACCCTCGCA CTTTTGTTTC TCTGGTTCCT
    ACATCTGCTC ATACTGGAGA TGGAATGGGA AATCTGATCT CTCTTCTTGT AGACTTGACA
    CAGACCATGC TTAATAAAAG GCTGGCTCAT TCCGAAGAGT TAAGAGCCCA AGTAATGGAA
    GTGAAAGCCC TGCCAGGGAT GGGAACGACT ATTGATGTCA TTTTGATCAA TGGGCGTTTA
    AAGGAAAGTG ACATTGTTAT TGTTCCTGGT GTTGAAGGCC CAATAGTCAC CCAGATCAGA
    GGACTTTTGC TACCTCCCCC AATGAAAGAA TTAAGAGTTA AGAATCAGTA TGAGAAACAC
    AAAGAAGTAA TAGCTGCTCA AGGAGTTAAG ATTCTTGGGA AAGACTTGGA AAAGGCATTG
    GCTGGGTTGC CACTGTTAGT GGCCCACAGT GAGGATGAGA TCCCAGTTCT GAGGGATGAG
    CTCATCCATG AATTAAAACA GACCTTGAAT GCCATAAAGT TAGAAGAAAA GGGTGTTTAT
    GTGCAGGCAT CTACCCTGGG TTCTTTAGAA GCACTGCTAG AGTTCCTGAA AACATCAGAA
    GTACCGTATG CTGGAATCAA CATTGGACCT GTTCACAAAA AGGATGTCAT GAAAGCTTCA
    GTGATGTTGG AACATGATCC ACAATATGCT GTGATTTTGG CTTTTGATGT AAGGATTGAA
    CGAGAAGCCC AAGAAATGGC TGATAGTTTA GGAGTTCGCA TTTTTAGTGC TGAAATCATC
    TACCACTTAT TTGATGCCTT TACTAAGTAC CGCCAAGACT ATAAGAAGCA GAAACAGGAA
    GAATTCAAGC ACATAGCAGT ATTTCCGTGT AAAATCCGAA TTCTCCCTCA GTTTATCTTC
    AATTCTCGAG ATCCAATTGT CATGGGAGTG GTGGTGGAAG CAGGACAAGT GAAACAGGGA
    ACGCCAATTT GTGTACCTAG TAAGAATTTT GTAGATATTG GGATAGTGAC CAGTATTGAA
    ATTAACCACA AGTCAGTTGA TATGGCAAAG AAAGGCCAGG AAGTGTGCAT TAAAATCGAG
    CCCATTCCTG GAGAGTCTCC TAAGATGTTT GGAAGACACT TTGAGGCTAC AGATTTCCTC
    ATTAGCAAGA TTACTCGGCA GTCAATTGAT GCTTTGAAAA ACTGGTTTAG AGACGAGATG
    CAGAAATCAG ACTGGCAACT TATAGTGGAA CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52480
    Clone ID Related Accession (Same CDS sequence) XM_031897015.1
    Accession Version XM_031897015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GGAAACAGTG TGGATAGTGA AAGCGATAAA
    GAAGCAACAG AAGAGGATGC CGGTGTGGAT GACTGGGAAG CTATAGTGAG CGATGAAGAA
    AGGGAGAAAG AGACAAAAAC TATTCACATT GAAGTTCAGG AGAAGAATGA AGAGGAAGAA
    GAGGAGGACG AAGATGAAGA GGATGAGGGA GAAAGTGAGG AAGAGGATGA GGATGAAGGG
    GATAGTGAAG GCACTGAAGA TGGAAAGTCT TCAGATGAAA AGCCGCAAGA ATCCCAAACT
    GTTGCACCAA AACTTCAAGA GAAGAAGCCT AGCAGAGAAC TAAGCTCCGA GTCTGAGTCT
    GAGTCTGATT CTGATGACGA TCGCACTAAA GAAGAGATAG CGTACGACAA AGCAAAGAGG
    AGAATAGAGA AAAGACGCCT TGAAAACCAG AAAACTGTAA ATATCCAGGT ACTCCGGGCA
    CCAGTAATAT GTGTCCTTGG GCATGTAGAC ACAGGAAAAA CAAAAATCTT GGATAAGCTG
    CGCCATACAC ATGTACAAGA CAGTGAAGCA GGTGGTATCA CTCAACAGAT TGGAGCAACC
    AACGTCCCCC TTGATGCTAT CAAAGAACAG GCTAAGATGG TTAAGAATTT TGACAGGGAG
    AATATAAAGA TTCCAGGAAT GCTTATCATA GATACACCTG GCCATGAATC CTTCAGTAAT
    TTGAGGAATC GTGGAAGCTC CCTTTGTGAT ATTGCCATTC TTGTTGTGGA TATTATGCAT
    GGATTGGAGC CGCAGACAAT AGAATCTCTG AACCTGCTAA AAAATAAGAA GTGCCCTTTT
    ATTGTGGCTC TGAACAAAAT CGACAGGCTG TATGACTGGA AAAAGGGCCC AGATGCTGAT
    GTTGCTGCAA CATTAAAGAA ACAGAAGAAA AATACTAAGG ATGAATTTGA GGAGCGAGCC
    AAGGCCATCA TTGTTGAATT TGCACAGCAG GGTTTGAATG CTGCTTTATT CTATGAAAAT
    AAAGACCCTC GCACTTTTGT TTCTCTGGTT CCTACATCTG CTCATACTGG AGATGGAATG
    GGAAATCTGA TCTCTCTTCT TGTAGACTTG ACACAGACCA TGCTTAATAA AAGGCTGGCT
    CATTCCGAAG AGTTAAGAGC CCAAGTAATG GAAGTGAAAG CCCTGCCAGG GATGGGAACG
    ACTATTGATG TCATTTTGAT CAATGGGCGT TTAAAGGAAA GTGACATTGT TATTGTTCCT
    GGTGTTGAAG GCCCAATAGT CACCCAGATC AGAGGACTTT TGCTACCTCC CCCAATGAAA
    GAATTAAGAG TTAAGAATCA GTATGAGAAA CACAAAGAAG TAATAGCTGC TCAAGGAGTT
    AAGATTCTTG GGAAAGACTT GGAAAAGGCA TTGGCTGGGT TGCCACTGTT AGTGGCCCAC
    AGTGAGGATG AGATCCCAGT TCTGAGGGAT GAGCTCATCC ATGAATTAAA ACAGACCTTG
    AATGCCATAA AGTTAGAAGA AAAGGGTGTT TATGTGCAGG CATCTACCCT GGGTTCTTTA
    GAAGCACTGC TAGAGTTCCT GAAAACATCA GAAGTACCGT ATGCTGGAAT CAACATTGGA
    CCTGTTCACA AAAAGGATGT CATGAAAGCT TCAGTGATGT TGGAACATGA TCCACAATAT
    GCTGTGATTT TGGCTTTTGA TGTAAGGATT GAACGAGAAG CCCAAGAAAT GGCTGATAGT
    TTAGGAGTTC GCATTTTTAG TGCTGAAATC ATCTACCACT TATTTGATGC CTTTACTAAG
    TACCGCCAAG ACTATAAGAA GCAGAAACAG GAAGAATTCA AGCACATAGC AGTATTTCCG
    TGTAAAATCC GAATTCTCCC TCAGTTTATC TTCAATTCTC GAGATCCAAT TGTCATGGGA
    GTGGTGGTGG AAGCAGGACA AGTGAAACAG GGAACGCCAA TTTGTGTACC TAGTAAGAAT
    TTTGTAGATA TTGGGATAGT GACCAGTATT GAAATTAACC ACAAGTCAGT TGATATGGCA
    AAGAAAGGCC AGGAAGTGTG CATTAAAATC GAGCCCATTC CTGGAGAGTC TCCTAAGATG
    TTTGGAAGAC ACTTTGAGGC TACAGATTTC CTCATTAGCA AGATTACTCG GCAGTCAATT
    GATGCTTTGA AAAACTGGTT TAGAGACGAG ATGCAGAAAT CAGACTGGCA ACTTATAGTG
    GAACTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752875.1
    CDS162..3791
    Translation

    Target ORF information:

    RefSeq Version XM_031897015.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GGAAACAGTG TGGATAGTGA AAGCGATAAA
    GAAGCAACAG AAGAGGATGC CGGTGTGGAT GACTGGGAAG CTATAGTGAG CGATGAAGAA
    AGGGAGAAAG AGACAAAAAC TATTCACATT GAAGTTCAGG AGAAGAATGA AGAGGAAGAA
    GAGGAGGACG AAGATGAAGA GGATGAGGGA GAAAGTGAGG AAGAGGATGA GGATGAAGGG
    GATAGTGAAG GCACTGAAGA TGGAAAGTCT TCAGATGAAA AGCCGCAAGA ATCCCAAACT
    GTTGCACCAA AACTTCAAGA GAAGAAGCCT AGCAGAGAAC TAAGCTCCGA GTCTGAGTCT
    GAGTCTGATT CTGATGACGA TCGCACTAAA GAAGAGATAG CGTACGACAA AGCAAAGAGG
    AGAATAGAGA AAAGACGCCT TGAAAACCAG AAAACTGTAA ATATCCAGGT ACTCCGGGCA
    CCAGTAATAT GTGTCCTTGG GCATGTAGAC ACAGGAAAAA CAAAAATCTT GGATAAGCTG
    CGCCATACAC ATGTACAAGA CAGTGAAGCA GGTGGTATCA CTCAACAGAT TGGAGCAACC
    AACGTCCCCC TTGATGCTAT CAAAGAACAG GCTAAGATGG TTAAGAATTT TGACAGGGAG
    AATATAAAGA TTCCAGGAAT GCTTATCATA GATACACCTG GCCATGAATC CTTCAGTAAT
    TTGAGGAATC GTGGAAGCTC CCTTTGTGAT ATTGCCATTC TTGTTGTGGA TATTATGCAT
    GGATTGGAGC CGCAGACAAT AGAATCTCTG AACCTGCTAA AAAATAAGAA GTGCCCTTTT
    ATTGTGGCTC TGAACAAAAT CGACAGGCTG TATGACTGGA AAAAGGGCCC AGATGCTGAT
    GTTGCTGCAA CATTAAAGAA ACAGAAGAAA AATACTAAGG ATGAATTTGA GGAGCGAGCC
    AAGGCCATCA TTGTTGAATT TGCACAGCAG GGTTTGAATG CTGCTTTATT CTATGAAAAT
    AAAGACCCTC GCACTTTTGT TTCTCTGGTT CCTACATCTG CTCATACTGG AGATGGAATG
    GGAAATCTGA TCTCTCTTCT TGTAGACTTG ACACAGACCA TGCTTAATAA AAGGCTGGCT
    CATTCCGAAG AGTTAAGAGC CCAAGTAATG GAAGTGAAAG CCCTGCCAGG GATGGGAACG
    ACTATTGATG TCATTTTGAT CAATGGGCGT TTAAAGGAAA GTGACATTGT TATTGTTCCT
    GGTGTTGAAG GCCCAATAGT CACCCAGATC AGAGGACTTT TGCTACCTCC CCCAATGAAA
    GAATTAAGAG TTAAGAATCA GTATGAGAAA CACAAAGAAG TAATAGCTGC TCAAGGAGTT
    AAGATTCTTG GGAAAGACTT GGAAAAGGCA TTGGCTGGGT TGCCACTGTT AGTGGCCCAC
    AGTGAGGATG AGATCCCAGT TCTGAGGGAT GAGCTCATCC ATGAATTAAA ACAGACCTTG
    AATGCCATAA AGTTAGAAGA AAAGGGTGTT TATGTGCAGG CATCTACCCT GGGTTCTTTA
    GAAGCACTGC TAGAGTTCCT GAAAACATCA GAAGTACCGT ATGCTGGAAT CAACATTGGA
    CCTGTTCACA AAAAGGATGT CATGAAAGCT TCAGTGATGT TGGAACATGA TCCACAATAT
    GCTGTGATTT TGGCTTTTGA TGTAAGGATT GAACGAGAAG CCCAAGAAAT GGCTGATAGT
    TTAGGAGTTC GCATTTTTAG TGCTGAAATC ATCTACCACT TATTTGATGC CTTTACTAAG
    TACCGCCAAG ACTATAAGAA GCAGAAACAG GAAGAATTCA AGCACATAGC AGTATTTCCG
    TGTAAAATCC GAATTCTCCC TCAGTTTATC TTCAATTCTC GAGATCCAAT TGTCATGGGA
    GTGGTGGTGG AAGCAGGACA AGTGAAACAG GGAACGCCAA TTTGTGTACC TAGTAAGAAT
    TTTGTAGATA TTGGGATAGT GACCAGTATT GAAATTAACC ACAAGTCAGT TGATATGGCA
    AAGAAAGGCC AGGAAGTGTG CATTAAAATC GAGCCCATTC CTGGAGAGTC TCCTAAGATG
    TTTGGAAGAC ACTTTGAGGC TACAGATTTC CTCATTAGCA AGATTACTCG GCAGTCAATT
    GATGCTTTGA AAAACTGGTT TAGAGACGAG ATGCAGAAAT CAGACTGGCA ACTTATAGTG
    GAACTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05868
    Clone ID Related Accession (Same CDS sequence) XM_002937859.4 , XM_002937859.5
    Accession Version XM_002937859.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 5B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937859.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GCAACAGAAG AGGATGCCGG TGTGGATGAC
    TGGGAAGCTA TAGTGAGCGA TGAAGAAAGG GAGAAAGAGA CAAAAACTAT TCACATTGAA
    GTTCAGGAGA AGAATGAAGA GGAAGAAGAG GAGGACGAAG ATGAAGAGGA TGAGGGAGAA
    AGTGAGGAAG AGGATGAGGA TGAAGGGGAT AGTGAAGGCA CTGAAGATGG AAAGTCTTCA
    GATGAAAAGC CGCAAGAATC CCAAACTGTT GCACCAAAAC TTCAAGAGAA GAAGCCTAGC
    AGAGAACTAA GCTCCGAGTC TGAGTCTGAG TCTGATTCTG ATGACGATCG CACTAAAGAA
    GAGATAGCGT ACGACAAAGC AAAGAGGAGA ATAGAGAAAA GACGCCTTGA AAACCAGAAA
    ACTGTAAATA TCCAGGTACT CCGGGCACCA GTAATATGTG TCCTTGGGCA TGTAGACACA
    GGAAAAACAA AAATCTTGGA TAAGCTGCGC CATACACATG TACAAGACAG TGAAGCAGGT
    GGTATCACTC AACAGATTGG AGCAACCAAC GTCCCCCTTG ATGCTATCAA AGAACAGGCT
    AAGATGGTTA AGAATTTTGA CAGGGAGAAT ATAAAGATTC CAGGAATGCT TATCATAGAT
    ACACCTGGCC ATGAATCCTT CAGTAATTTG AGGAATCGTG GAAGCTCCCT TTGTGATATT
    GCCATTCTTG TTGTGGATAT TATGCATGGA TTGGAGCCGC AGACAATAGA ATCTCTGAAC
    CTGCTAAAAA ATAAGAAGTG CCCTTTTATT GTGGCTCTGA ACAAAATCGA CAGGCTGTAT
    GACTGGAAAA AGGGCCCAGA TGCTGATGTT GCTGCAACAT TAAAGAAACA GAAGAAAAAT
    ACTAAGGATG AATTTGAGGA GCGAGCCAAG GCCATCATTG TTGAATTTGC ACAGCAGGGT
    TTGAATGCTG CTTTATTCTA TGAAAATAAA GACCCTCGCA CTTTTGTTTC TCTGGTTCCT
    ACATCTGCTC ATACTGGAGA TGGAATGGGA AATCTGATCT CTCTTCTTGT AGACTTGACA
    CAGACCATGC TTAATAAAAG GCTGGCTCAT TCCGAAGAGT TAAGAGCCCA AGTAATGGAA
    GTGAAAGCCC TGCCAGGGAT GGGAACGACT ATTGATGTCA TTTTGATCAA TGGGCGTTTA
    AAGGAAAGTG ACATTGTTAT TGTTCCTGGT GTTGAAGGCC CAATAGTCAC CCAGATCAGA
    GGACTTTTGC TACCTCCCCC AATGAAAGAA TTAAGAGTTA AGAATCAGTA TGAGAAACAC
    AAAGAAGTAA TAGCTGCTCA AGGAGTTAAG ATTCTTGGGA AAGACTTGGA AAAGGCATTG
    GCTGGGTTGC CACTGTTAGT GGCCCACAGT GAGGATGAGA TCCCAGTTCT GAGGGATGAG
    CTCATCCATG AATTAAAACA GACCTTGAAT GCCATAAAGT TAGAAGAAAA GGGTGTTTAT
    GTGCAGGCAT CTACCCTGGG TTCTTTAGAA GCACTGCTAG AGTTCCTGAA AACATCAGAA
    GTACCGTATG CTGGAATCAA CATTGGACCT GTTCACAAAA AGGATGTCAT GAAAGCTTCA
    GTGATGTTGG AACATGATCC ACAATATGCT GTGATTTTGG CTTTTGATGT AAGGATTGAA
    CGAGAAGCCC AAGAAATGGC TGATAGTTTA GGAGTTCGCA TTTTTAGTGC TGAAATCATC
    TACCACTTAT TTGATGCCTT TACTAAGTAC CGCCAAGACT ATAAGAAGCA GAAACAGGAA
    GAATTCAAGC ACATAGCAGT ATTTCCGTGT AAAATCCGAA TTCTCCCTCA GTTTATCTTC
    AATTCTCGAG ATCCAATTGT CATGGGAGTG GTGGTGGAAG CAGGACAAGT GAAACAGGGA
    ACGCCAATTT GTGTACCTAG TAAGAATTTT GTAGATATTG GGATAGTGAC CAGTATTGAA
    ATTAACCACA AGTCAGTTGA TATGGCAAAG AAAGGCCAGG AAGTGTGCAT TAAAATCGAG
    CCCATTCCTG GAGAGTCTCC TAAGATGTTT GGAAGACACT TTGAGGCTAC AGATTTCCTC
    ATTAGCAAGA TTACTCGGCA GTCAATTGAT GCTTTGAAAA ACTGGTTTAG AGACGAGATG
    CAGAAATCAG ACTGGCAACT TATAGTGGAA CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937905.1
    CDS172..3768
    Translation

    Target ORF information:

    RefSeq Version XM_002937859.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 5B (eif5b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937859.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAGA AACAGAAGAC CAAGAGTGAT GAGAGTGCCA AGGATGATGT TGATGTTGAT 
    GCCTTAGCTG CAGAGATTGA GGGAGCTGGA GCTGCTAAAG ACCAAAAGGC CAAAGGAAAA
    AAGAAGAAAG GAAAGAAGCA AGATTTTAAT GAAGAAGATA TATTGAAGGA ACTGGAGGAG
    CTGTCCTTGG AAGCACAAGG GGGGAAAGGA GATAAAGAAC TTGTTTCAAA GAAAGCAGAA
    ACTGACAATA TTGAACCTGT ACCCGTCAAG CAAGACAAGA AAAAGAAAGG CCAAAAAGCC
    AAGAAGACAA ATTTGGATGA AGATGAGGAC AATAGTGAAA ATGAAATGGA GACCAAAACC
    GCAAAACCCA AGAAAGCAGA TAAATCTAAA CCCATTGACA TGTCTGAGAG TGATGATGAT
    AATGATAACT TTAATCAAAC TTCTAAGAAG GCAAAGGGAA AAAATCAATC CGCTTCTAAA
    AAACTTCAAG TTGTCGAGGA AGCTAATGAT CAAAAAGAAG AACACTCTGG AGACAGTGGT
    GAGGAGTCAG ATGAGTTTAG CCAAGGGAAG AAAGGAAAGA AAAATCAAAA GGCAAAGTCT
    GCACCAAAAG TAGAAAGTGC AGATGAAGAT GGGGAATTCA AGATCAAGAC TGCTGCTCAA
    AAGAAGGCTG AGAAGAAAGA ACGAGAAAGA AAAAAACGAG AGGAAGAAAA GGCTAAAGTA
    AAAAAGCAGA AAGAAAAGGA TGAACCTGAA GGTGGGAAAA AAGATGCTGC TGAGACAGCT
    GCTCCCAAAA AGGAAGCCAA GGCTGCAAAT GAGCTTGAAA TCACAACTGC TGCAGCTGCA
    GAAGGAGAAG CAGATGATGA CAATGAGGGA GACAAAAAGA AAAAAGACAA AAAGAAAAAG
    AAAGTGGAAA AGACAGAAAA AGATGATAAG AAAAAGCGAC CCAGTACAGC TGTTGTTAAA
    GCCATGCAGG AAGCTCTGGC TAAAATGAAA GAAGAGGAGG AGAGACTGAA GAGAGAAGAG
    GAAGAGCGTT TAAGACGGCT GGAGGAAATG GAAGCCAAGC GTTTGGAAGA GGAACGACTG
    GAGCAAGAGC GAAAAGAAAA AAGAAAGCAA AAAGAAAAAG AAAGAAAGGA ACGCTTAAAA
    AAGGAGGGCA AGCTACTGAC CAAAACACAG AGAGAAGCCA GAGCAAGAGC AGAAGCAACT
    CTGAAGGTCC TCCAAGCTCA GGGTGTTGAG GTACCTTCCA AAGACTCTGT GCCAAAGAAA
    AAGCCAATTT ATGAAGACAA AAAGAAAAAG AAGCCACAAC AACAGACACC AGTTAAGCAA
    GAGCCAATGG AGGTGAGCTC TCCGGTAGAA GCAGTTATAG AACAACCAGC ACCAGCGAAA
    GAGGAGACGG CAGTGCCAGT GGAATCACCT GCAACAGAAG AGGATGCCGG TGTGGATGAC
    TGGGAAGCTA TAGTGAGCGA TGAAGAAAGG GAGAAAGAGA CAAAAACTAT TCACATTGAA
    GTTCAGGAGA AGAATGAAGA GGAAGAAGAG GAGGACGAAG ATGAAGAGGA TGAGGGAGAA
    AGTGAGGAAG AGGATGAGGA TGAAGGGGAT AGTGAAGGCA CTGAAGATGG AAAGTCTTCA
    GATGAAAAGC CGCAAGAATC CCAAACTGTT GCACCAAAAC TTCAAGAGAA GAAGCCTAGC
    AGAGAACTAA GCTCCGAGTC TGAGTCTGAG TCTGATTCTG ATGACGATCG CACTAAAGAA
    GAGATAGCGT ACGACAAAGC AAAGAGGAGA ATAGAGAAAA GACGCCTTGA AAACCAGAAA
    ACTGTAAATA TCCAGGTACT CCGGGCACCA GTAATATGTG TCCTTGGGCA TGTAGACACA
    GGAAAAACAA AAATCTTGGA TAAGCTGCGC CATACACATG TACAAGACAG TGAAGCAGGT
    GGTATCACTC AACAGATTGG AGCAACCAAC GTCCCCCTTG ATGCTATCAA AGAACAGGCT
    AAGATGGTTA AGAATTTTGA CAGGGAGAAT ATAAAGATTC CAGGAATGCT TATCATAGAT
    ACACCTGGCC ATGAATCCTT CAGTAATTTG AGGAATCGTG GAAGCTCCCT TTGTGATATT
    GCCATTCTTG TTGTGGATAT TATGCATGGA TTGGAGCCGC AGACAATAGA ATCTCTGAAC
    CTGCTAAAAA ATAAGAAGTG CCCTTTTATT GTGGCTCTGA ACAAAATCGA CAGGCTGTAT
    GACTGGAAAA AGGGCCCAGA TGCTGATGTT GCTGCAACAT TAAAGAAACA GAAGAAAAAT
    ACTAAGGATG AATTTGAGGA GCGAGCCAAG GCCATCATTG TTGAATTTGC ACAGCAGGGT
    TTGAATGCTG CTTTATTCTA TGAAAATAAA GACCCTCGCA CTTTTGTTTC TCTGGTTCCT
    ACATCTGCTC ATACTGGAGA TGGAATGGGA AATCTGATCT CTCTTCTTGT AGACTTGACA
    CAGACCATGC TTAATAAAAG GCTGGCTCAT TCCGAAGAGT TAAGAGCCCA AGTAATGGAA
    GTGAAAGCCC TGCCAGGGAT GGGAACGACT ATTGATGTCA TTTTGATCAA TGGGCGTTTA
    AAGGAAAGTG ACATTGTTAT TGTTCCTGGT GTTGAAGGCC CAATAGTCAC CCAGATCAGA
    GGACTTTTGC TACCTCCCCC AATGAAAGAA TTAAGAGTTA AGAATCAGTA TGAGAAACAC
    AAAGAAGTAA TAGCTGCTCA AGGAGTTAAG ATTCTTGGGA AAGACTTGGA AAAGGCATTG
    GCTGGGTTGC CACTGTTAGT GGCCCACAGT GAGGATGAGA TCCCAGTTCT GAGGGATGAG
    CTCATCCATG AATTAAAACA GACCTTGAAT GCCATAAAGT TAGAAGAAAA GGGTGTTTAT
    GTGCAGGCAT CTACCCTGGG TTCTTTAGAA GCACTGCTAG AGTTCCTGAA AACATCAGAA
    GTACCGTATG CTGGAATCAA CATTGGACCT GTTCACAAAA AGGATGTCAT GAAAGCTTCA
    GTGATGTTGG AACATGATCC ACAATATGCT GTGATTTTGG CTTTTGATGT AAGGATTGAA
    CGAGAAGCCC AAGAAATGGC TGATAGTTTA GGAGTTCGCA TTTTTAGTGC TGAAATCATC
    TACCACTTAT TTGATGCCTT TACTAAGTAC CGCCAAGACT ATAAGAAGCA GAAACAGGAA
    GAATTCAAGC ACATAGCAGT ATTTCCGTGT AAAATCCGAA TTCTCCCTCA GTTTATCTTC
    AATTCTCGAG ATCCAATTGT CATGGGAGTG GTGGTGGAAG CAGGACAAGT GAAACAGGGA
    ACGCCAATTT GTGTACCTAG TAAGAATTTT GTAGATATTG GGATAGTGAC CAGTATTGAA
    ATTAACCACA AGTCAGTTGA TATGGCAAAG AAAGGCCAGG AAGTGTGCAT TAAAATCGAG
    CCCATTCCTG GAGAGTCTCC TAAGATGTTT GGAAGACACT TTGAGGCTAC AGATTTCCTC
    ATTAGCAAGA TTACTCGGCA GTCAATTGAT GCTTTGAAAA ACTGGTTTAG AGACGAGATG
    CAGAAATCAG ACTGGCAACT TATAGTGGAA CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.