EIF5B cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF5B
Entrez Gene ID	474556
Full Name	eukaryotic translation initiation factor 5B
General protein information	Preferred Names eukaryotic translation initiation factor 5B Names eukaryotic translation initiation factor 5B
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005626021.3	XP_005626078.1	eukaryotic translation initiation factor 5B isoform X1
XM_005626022.3	XP_005626079.1	eukaryotic translation initiation factor 5B isoform X2
XM_005626021.2	XP_005626078.1	eukaryotic translation initiation factor 5B isoform X1
XM_005626022.2	XP_005626079.1	eukaryotic translation initiation factor 5B isoform X2
XM_005626023.2	XP_005626080.1	eukaryotic translation initiation factor 5B isoform X3
XM_005626023.3	XP_005626080.1	eukaryotic translation initiation factor 5B isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF5B	NP_056988.3
Rattus norvegicus (Norway rat)	Eif5b	NP_001103611.1
Bos taurus (cattle)	EIF5B	XP_003586655.1
Gallus gallus (chicken)	EIF5B	NP_001033585.1
Pan troglodytes (chimpanzee)	EIF5B	XP_001159759.1
Canis lupus familiaris (dog)	EIF5B	XP_005626079.1
	EIF5B	XP_001103720.2
Xenopus tropicalis (tropical clawed frog)	eif5b	XP_004911863.1
Mus musculus (house mouse)	Eif5b	NP_938045.2
Danio rerio (zebrafish)	eif5b	XP_001923573.4

Gene Ontology

The following EIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe13661	XM_005626021.3 Latest version!	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13662	XM_005626022.3 Latest version!	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13663	XM_005626023.3 Latest version!	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCe13661	XM_005626021.2	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13662	XM_005626022.2	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13663	XM_005626023.2	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF5B ( XM_005626021.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626078.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

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CloneID	OCe13661
Clone ID Related Accession (Same CDS sequence)	XM_005626021.3 , XM_005626021.2
Accession Version	XM_005626021.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	eukaryotic translation initiation factor 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAGAAAAT 
ACTGTGCATA TAGAAGTAAA AGAAAACCCT GAGGAGGAAG AGGAAGAGGA GGAAGAAGAG 
AGTGAAGAAG AAGAGAGTGA AGAGGAAGAG GAAGAGGAAG GAGAAAGTGA AGGCAGTGAA 
GGTGATGAAG AAGATGAAAA GGTGTCCGAT GAGAAGGATG CAGGGAAAAT GTTAGATAAA 
AAACCAAGTA AAGAAATGAG TTCAGAATCT GAATACGACT CTGATGATGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCCAAACGG AGGATTGAGA AACGGCGACT TGAACATAGT 
AAAAATGTAA ATACAGAAAA GCTAAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAC 
ACAGGGAAGA CAAAGATTCT AGATAAACTT CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGCATTA CTCAACAAAT TGGGGCCACC AATGTTCCCC TTGAAGCTAT TAATGAACAG 
ACTAAGATGA TTAAAAATTT TGATAGAGAG AACGTACGAA TTCCAGGAAT GCTCATTATT 
GATACTCCTG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAT 
ATTGCTATTT TAGTTGTTGA CATTATGCAT GGTTTGGAGC CTCAGACAAT TGAATCTATT 
AATCTTCTGA AATCTAAAAA ATGCCCCTTC ATTGTTGCAC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAT GTGGCTGCTA CTTTAAAGAA GCAGAAAAAG 
AATACAAAAG ATGAATTTGA GGAGCGAGCA AAGGCTATTA TTGTGGAATT TGCACAGCAG 
GGTTTGAATG CAGCTTTGTT TTATGAGAAT AAGGATCCCC GTACTTTTGT GTCCTTGGTA 
CCTACCTCTG CGCATACTGG TGATGGTATG GGAAGTCTGA TCTACCTTCT TGTTGAGTTA 
ACTCAGACCA TGTTAAGCAA GAGACTTGCA CACTGTGAGG AGCTAAGAGC ACAGGTTATG 
GAGGTTAAGG CTCTACCAGG AATGGGCACC ACTATAGATG TGATACTGAT CAATGGGCGT 
TTGAAGGAAG GAGATACAAT TATTGTTCCT GGAGTAGAAG GGCCCATTGT AACTCAGATT 
CGAGGCCTCC TATTACCTCC TCCTATGAAG GAATTAAGGG TGAAGAACCA GTATGAAAAG 
CATAAAGAAG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAAGACCT AGAGAAGACA 
TTGGCTGGTT TACCCCTCCT GGTGGCTTAT AAAGAAGATG AAATCCCAGT TCTTAAAGAT 
GAGTTGATCC ATGAGTTAAA GCAGACGCTA AATGCTATCA AGTTGGAAGA AAAAGGAGTC 
TATGTTCAAG CATCTACACT GGGATCTTTG GAAGCTCTAC TTGAATTTCT AAAAACATCA 
GAAGTGCCCT ATGCAGGAAT TAACATTGGT CCAGTCCATA AAAAAGATGT TATGAAGGCT 
TCAGTGATGC TAGAACATGA TCCCCAGTAT GCAGTAATTT TGGCCTTTGA TGTGAGAATT 
GAACGGGATG CACAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TATAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCATATAGC AGTATTTCCC TGCAAGATGA AAATCCTCCC CCAGTATATT 
TTTAATTCTC GAGACCCAAT AGTGATGGGA GTGACTGTGG AAGCAGGTCA GGTGAAACAG 
GGGACACCCA TGTGCGTCCC TAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAATAAACC ATAAACAAGT GGATGTCGCA AAAAAAGGAC AAGAAGTCTG TGTCAAAATA 
GAACCTATCC CTGGTGAATC TCCCAAAATG TATGGAAGAC ATTTTGAAGC TACAGACATT 
CTTGTCAGTA AGATCAGCCG GCAGTCCATC GATGCCCTCA AGGACTGGTT CAGAGATGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTATTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626078.1
CDS	201..3860
Translation	MGKKQKNKSEDSAKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADRESAAVKPTENNEEESTSKQDKKKKGQKGKKQSFDDNDSEEWEDKDSKSKKTAKSKVEMYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKEENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626021.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626021.3

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAGAAAAT 
ACTGTGCATA TAGAAGTAAA AGAAAACCCT GAGGAGGAAG AGGAAGAGGA GGAAGAAGAG 
AGTGAAGAAG AAGAGAGTGA AGAGGAAGAG GAAGAGGAAG GAGAAAGTGA AGGCAGTGAA 
GGTGATGAAG AAGATGAAAA GGTGTCCGAT GAGAAGGATG CAGGGAAAAT GTTAGATAAA 
AAACCAAGTA AAGAAATGAG TTCAGAATCT GAATACGACT CTGATGATGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCCAAACGG AGGATTGAGA AACGGCGACT TGAACATAGT 
AAAAATGTAA ATACAGAAAA GCTAAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAC 
ACAGGGAAGA CAAAGATTCT AGATAAACTT CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGCATTA CTCAACAAAT TGGGGCCACC AATGTTCCCC TTGAAGCTAT TAATGAACAG 
ACTAAGATGA TTAAAAATTT TGATAGAGAG AACGTACGAA TTCCAGGAAT GCTCATTATT 
GATACTCCTG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAT 
ATTGCTATTT TAGTTGTTGA CATTATGCAT GGTTTGGAGC CTCAGACAAT TGAATCTATT 
AATCTTCTGA AATCTAAAAA ATGCCCCTTC ATTGTTGCAC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAT GTGGCTGCTA CTTTAAAGAA GCAGAAAAAG 
AATACAAAAG ATGAATTTGA GGAGCGAGCA AAGGCTATTA TTGTGGAATT TGCACAGCAG 
GGTTTGAATG CAGCTTTGTT TTATGAGAAT AAGGATCCCC GTACTTTTGT GTCCTTGGTA 
CCTACCTCTG CGCATACTGG TGATGGTATG GGAAGTCTGA TCTACCTTCT TGTTGAGTTA 
ACTCAGACCA TGTTAAGCAA GAGACTTGCA CACTGTGAGG AGCTAAGAGC ACAGGTTATG 
GAGGTTAAGG CTCTACCAGG AATGGGCACC ACTATAGATG TGATACTGAT CAATGGGCGT 
TTGAAGGAAG GAGATACAAT TATTGTTCCT GGAGTAGAAG GGCCCATTGT AACTCAGATT 
CGAGGCCTCC TATTACCTCC TCCTATGAAG GAATTAAGGG TGAAGAACCA GTATGAAAAG 
CATAAAGAAG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAAGACCT AGAGAAGACA 
TTGGCTGGTT TACCCCTCCT GGTGGCTTAT AAAGAAGATG AAATCCCAGT TCTTAAAGAT 
GAGTTGATCC ATGAGTTAAA GCAGACGCTA AATGCTATCA AGTTGGAAGA AAAAGGAGTC 
TATGTTCAAG CATCTACACT GGGATCTTTG GAAGCTCTAC TTGAATTTCT AAAAACATCA 
GAAGTGCCCT ATGCAGGAAT TAACATTGGT CCAGTCCATA AAAAAGATGT TATGAAGGCT 
TCAGTGATGC TAGAACATGA TCCCCAGTAT GCAGTAATTT TGGCCTTTGA TGTGAGAATT 
GAACGGGATG CACAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TATAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCATATAGC AGTATTTCCC TGCAAGATGA AAATCCTCCC CCAGTATATT 
TTTAATTCTC GAGACCCAAT AGTGATGGGA GTGACTGTGG AAGCAGGTCA GGTGAAACAG 
GGGACACCCA TGTGCGTCCC TAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAATAAACC ATAAACAAGT GGATGTCGCA AAAAAAGGAC AAGAAGTCTG TGTCAAAATA 
GAACCTATCC CTGGTGAATC TCCCAAAATG TATGGAAGAC ATTTTGAAGC TACAGACATT 
CTTGTCAGTA AGATCAGCCG GCAGTCCATC GATGCCCTCA AGGACTGGTT CAGAGATGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTATTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_005626021.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626078.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe13661
Clone ID Related Accession (Same CDS sequence)	XM_005626021.3 , XM_005626021.2
Accession Version	XM_005626021.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	eukaryotic translation initiation factor 5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAGAAAAT 
ACTGTGCATA TAGAAGTAAA AGAAAACCCT GAGGAGGAAG AGGAAGAGGA GGAAGAAGAG 
AGTGAAGAAG AAGAGAGTGA AGAGGAAGAG GAAGAGGAAG GAGAAAGTGA AGGCAGTGAA 
GGTGATGAAG AAGATGAAAA GGTGTCCGAT GAGAAGGATG CAGGGAAAAT GTTAGATAAA 
AAACCAAGTA AAGAAATGAG TTCAGAATCT GAATACGACT CTGATGATGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCCAAACGG AGGATTGAGA AACGGCGACT TGAACATAGT 
AAAAATGTAA ATACAGAAAA GCTAAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAC 
ACAGGGAAGA CAAAGATTCT AGATAAACTT CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGCATTA CTCAACAAAT TGGGGCCACC AATGTTCCCC TTGAAGCTAT TAATGAACAG 
ACTAAGATGA TTAAAAATTT TGATAGAGAG AACGTACGAA TTCCAGGAAT GCTCATTATT 
GATACTCCTG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAT 
ATTGCTATTT TAGTTGTTGA CATTATGCAT GGTTTGGAGC CTCAGACAAT TGAATCTATT 
AATCTTCTGA AATCTAAAAA ATGCCCCTTC ATTGTTGCAC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAT GTGGCTGCTA CTTTAAAGAA GCAGAAAAAG 
AATACAAAAG ATGAATTTGA GGAGCGAGCA AAGGCTATTA TTGTGGAATT TGCACAGCAG 
GGTTTGAATG CAGCTTTGTT TTATGAGAAT AAGGATCCCC GTACTTTTGT GTCCTTGGTA 
CCTACCTCTG CGCATACTGG TGATGGTATG GGAAGTCTGA TCTACCTTCT TGTTGAGTTA 
ACTCAGACCA TGTTAAGCAA GAGACTTGCA CACTGTGAGG AGCTAAGAGC ACAGGTTATG 
GAGGTTAAGG CTCTACCAGG AATGGGCACC ACTATAGATG TGATACTGAT CAATGGGCGT 
TTGAAGGAAG GAGATACAAT TATTGTTCCT GGAGTAGAAG GGCCCATTGT AACTCAGATT 
CGAGGCCTCC TATTACCTCC TCCTATGAAG GAATTAAGGG TGAAGAACCA GTATGAAAAG 
CATAAAGAAG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAAGACCT AGAGAAGACA 
TTGGCTGGTT TACCCCTCCT GGTGGCTTAT AAAGAAGATG AAATCCCAGT TCTTAAAGAT 
GAGTTGATCC ATGAGTTAAA GCAGACGCTA AATGCTATCA AGTTGGAAGA AAAAGGAGTC 
TATGTTCAAG CATCTACACT GGGATCTTTG GAAGCTCTAC TTGAATTTCT AAAAACATCA 
GAAGTGCCCT ATGCAGGAAT TAACATTGGT CCAGTCCATA AAAAAGATGT TATGAAGGCT 
TCAGTGATGC TAGAACATGA TCCCCAGTAT GCAGTAATTT TGGCCTTTGA TGTGAGAATT 
GAACGGGATG CACAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TATAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCATATAGC AGTATTTCCC TGCAAGATGA AAATCCTCCC CCAGTATATT 
TTTAATTCTC GAGACCCAAT AGTGATGGGA GTGACTGTGG AAGCAGGTCA GGTGAAACAG 
GGGACACCCA TGTGCGTCCC TAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAATAAACC ATAAACAAGT GGATGTCGCA AAAAAAGGAC AAGAAGTCTG TGTCAAAATA 
GAACCTATCC CTGGTGAATC TCCCAAAATG TATGGAAGAC ATTTTGAAGC TACAGACATT 
CTTGTCAGTA AGATCAGCCG GCAGTCCATC GATGCCCTCA AGGACTGGTT CAGAGATGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTATTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626078.1
CDS	169..3828
Translation	MGKKQKNKSEDSAKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADRESAAVKPTENNEEESTSKQDKKKKGQKGKKQSFDDNDSEEWEDKDSKSKKTAKSKVEMYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKEENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626021.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626021.2

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAGAAAAT 
ACTGTGCATA TAGAAGTAAA AGAAAACCCT GAGGAGGAAG AGGAAGAGGA GGAAGAAGAG 
AGTGAAGAAG AAGAGAGTGA AGAGGAAGAG GAAGAGGAAG GAGAAAGTGA AGGCAGTGAA 
GGTGATGAAG AAGATGAAAA GGTGTCCGAT GAGAAGGATG CAGGGAAAAT GTTAGATAAA 
AAACCAAGTA AAGAAATGAG TTCAGAATCT GAATACGACT CTGATGATGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCCAAACGG AGGATTGAGA AACGGCGACT TGAACATAGT 
AAAAATGTAA ATACAGAAAA GCTAAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAC 
ACAGGGAAGA CAAAGATTCT AGATAAACTT CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGCATTA CTCAACAAAT TGGGGCCACC AATGTTCCCC TTGAAGCTAT TAATGAACAG 
ACTAAGATGA TTAAAAATTT TGATAGAGAG AACGTACGAA TTCCAGGAAT GCTCATTATT 
GATACTCCTG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAT 
ATTGCTATTT TAGTTGTTGA CATTATGCAT GGTTTGGAGC CTCAGACAAT TGAATCTATT 
AATCTTCTGA AATCTAAAAA ATGCCCCTTC ATTGTTGCAC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAT GTGGCTGCTA CTTTAAAGAA GCAGAAAAAG 
AATACAAAAG ATGAATTTGA GGAGCGAGCA AAGGCTATTA TTGTGGAATT TGCACAGCAG 
GGTTTGAATG CAGCTTTGTT TTATGAGAAT AAGGATCCCC GTACTTTTGT GTCCTTGGTA 
CCTACCTCTG CGCATACTGG TGATGGTATG GGAAGTCTGA TCTACCTTCT TGTTGAGTTA 
ACTCAGACCA TGTTAAGCAA GAGACTTGCA CACTGTGAGG AGCTAAGAGC ACAGGTTATG 
GAGGTTAAGG CTCTACCAGG AATGGGCACC ACTATAGATG TGATACTGAT CAATGGGCGT 
TTGAAGGAAG GAGATACAAT TATTGTTCCT GGAGTAGAAG GGCCCATTGT AACTCAGATT 
CGAGGCCTCC TATTACCTCC TCCTATGAAG GAATTAAGGG TGAAGAACCA GTATGAAAAG 
CATAAAGAAG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAAGACCT AGAGAAGACA 
TTGGCTGGTT TACCCCTCCT GGTGGCTTAT AAAGAAGATG AAATCCCAGT TCTTAAAGAT 
GAGTTGATCC ATGAGTTAAA GCAGACGCTA AATGCTATCA AGTTGGAAGA AAAAGGAGTC 
TATGTTCAAG CATCTACACT GGGATCTTTG GAAGCTCTAC TTGAATTTCT AAAAACATCA 
GAAGTGCCCT ATGCAGGAAT TAACATTGGT CCAGTCCATA AAAAAGATGT TATGAAGGCT 
TCAGTGATGC TAGAACATGA TCCCCAGTAT GCAGTAATTT TGGCCTTTGA TGTGAGAATT 
GAACGGGATG CACAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TATAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCATATAGC AGTATTTCCC TGCAAGATGA AAATCCTCCC CCAGTATATT 
TTTAATTCTC GAGACCCAAT AGTGATGGGA GTGACTGTGG AAGCAGGTCA GGTGAAACAG 
GGGACACCCA TGTGCGTCCC TAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAATAAACC ATAAACAAGT GGATGTCGCA AAAAAAGGAC AAGAAGTCTG TGTCAAAATA 
GAACCTATCC CTGGTGAATC TCCCAAAATG TATGGAAGAC ATTTTGAAGC TACAGACATT 
CTTGTCAGTA AGATCAGCCG GCAGTCCATC GATGCCCTCA AGGACTGGTT CAGAGATGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTATTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_005626022.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626079.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

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CloneID	OCe13662
Clone ID Related Accession (Same CDS sequence)	XM_005626022.3 , XM_005626022.2
Accession Version	XM_005626022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	eukaryotic translation initiation factor 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAAATACT 
GTGCATATAG AAGTAAAAGA AAACCCTGAG GAGGAAGAGG AAGAGGAGGA AGAAGAGAGT 
GAAGAAGAAG AGAGTGAAGA GGAAGAGGAA GAGGAAGGAG AAAGTGAAGG CAGTGAAGGT 
GATGAAGAAG ATGAAAAGGT GTCCGATGAG AAGGATGCAG GGAAAATGTT AGATAAAAAA 
CCAAGTAAAG AAATGAGTTC AGAATCTGAA TACGACTCTG ATGATGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC CAAACGGAGG ATTGAGAAAC GGCGACTTGA ACATAGTAAA 
AATGTAAATA CAGAAAAGCT AAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGACACA 
GGGAAGACAA AGATTCTAGA TAAACTTCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGCATTACTC AACAAATTGG GGCCACCAAT GTTCCCCTTG AAGCTATTAA TGAACAGACT 
AAGATGATTA AAAATTTTGA TAGAGAGAAC GTACGAATTC CAGGAATGCT CATTATTGAT 
ACTCCTGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGATATT 
GCTATTTTAG TTGTTGACAT TATGCATGGT TTGGAGCCTC AGACAATTGA ATCTATTAAT 
CTTCTGAAAT CTAAAAAATG CCCCTTCATT GTTGCACTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGATGTG GCTGCTACTT TAAAGAAGCA GAAAAAGAAT 
ACAAAAGATG AATTTGAGGA GCGAGCAAAG GCTATTATTG TGGAATTTGC ACAGCAGGGT 
TTGAATGCAG CTTTGTTTTA TGAGAATAAG GATCCCCGTA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCGC ATACTGGTGA TGGTATGGGA AGTCTGATCT ACCTTCTTGT TGAGTTAACT 
CAGACCATGT TAAGCAAGAG ACTTGCACAC TGTGAGGAGC TAAGAGCACA GGTTATGGAG 
GTTAAGGCTC TACCAGGAAT GGGCACCACT ATAGATGTGA TACTGATCAA TGGGCGTTTG 
AAGGAAGGAG ATACAATTAT TGTTCCTGGA GTAGAAGGGC CCATTGTAAC TCAGATTCGA 
GGCCTCCTAT TACCTCCTCC TATGAAGGAA TTAAGGGTGA AGAACCAGTA TGAAAAGCAT 
AAAGAAGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AAGACCTAGA GAAGACATTG 
GCTGGTTTAC CCCTCCTGGT GGCTTATAAA GAAGATGAAA TCCCAGTTCT TAAAGATGAG 
TTGATCCATG AGTTAAAGCA GACGCTAAAT GCTATCAAGT TGGAAGAAAA AGGAGTCTAT 
GTTCAAGCAT CTACACTGGG ATCTTTGGAA GCTCTACTTG AATTTCTAAA AACATCAGAA 
GTGCCCTATG CAGGAATTAA CATTGGTCCA GTCCATAAAA AAGATGTTAT GAAGGCTTCA 
GTGATGCTAG AACATGATCC CCAGTATGCA GTAATTTTGG CCTTTGATGT GAGAATTGAA 
CGGGATGCAC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAT AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ATATAGCAGT ATTTCCCTGC AAGATGAAAA TCCTCCCCCA GTATATTTTT 
AATTCTCGAG ACCCAATAGT GATGGGAGTG ACTGTGGAAG CAGGTCAGGT GAAACAGGGG 
ACACCCATGT GCGTCCCTAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
ATAAACCATA AACAAGTGGA TGTCGCAAAA AAAGGACAAG AAGTCTGTGT CAAAATAGAA 
CCTATCCCTG GTGAATCTCC CAAAATGTAT GGAAGACATT TTGAAGCTAC AGACATTCTT 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCCCTCAAGG ACTGGTTCAG AGATGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TATTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626079.1
CDS	201..3857
Translation	MGKKQKNKSEDSAKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADRESAAVKPTENNEEESTSKQDKKKKGQKGKKQSFDDNDSEEWEDKDSKSKKTAKSKVEMYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626022.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626022.3

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAAATACT 
GTGCATATAG AAGTAAAAGA AAACCCTGAG GAGGAAGAGG AAGAGGAGGA AGAAGAGAGT 
GAAGAAGAAG AGAGTGAAGA GGAAGAGGAA GAGGAAGGAG AAAGTGAAGG CAGTGAAGGT 
GATGAAGAAG ATGAAAAGGT GTCCGATGAG AAGGATGCAG GGAAAATGTT AGATAAAAAA 
CCAAGTAAAG AAATGAGTTC AGAATCTGAA TACGACTCTG ATGATGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC CAAACGGAGG ATTGAGAAAC GGCGACTTGA ACATAGTAAA 
AATGTAAATA CAGAAAAGCT AAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGACACA 
GGGAAGACAA AGATTCTAGA TAAACTTCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGCATTACTC AACAAATTGG GGCCACCAAT GTTCCCCTTG AAGCTATTAA TGAACAGACT 
AAGATGATTA AAAATTTTGA TAGAGAGAAC GTACGAATTC CAGGAATGCT CATTATTGAT 
ACTCCTGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGATATT 
GCTATTTTAG TTGTTGACAT TATGCATGGT TTGGAGCCTC AGACAATTGA ATCTATTAAT 
CTTCTGAAAT CTAAAAAATG CCCCTTCATT GTTGCACTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGATGTG GCTGCTACTT TAAAGAAGCA GAAAAAGAAT 
ACAAAAGATG AATTTGAGGA GCGAGCAAAG GCTATTATTG TGGAATTTGC ACAGCAGGGT 
TTGAATGCAG CTTTGTTTTA TGAGAATAAG GATCCCCGTA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCGC ATACTGGTGA TGGTATGGGA AGTCTGATCT ACCTTCTTGT TGAGTTAACT 
CAGACCATGT TAAGCAAGAG ACTTGCACAC TGTGAGGAGC TAAGAGCACA GGTTATGGAG 
GTTAAGGCTC TACCAGGAAT GGGCACCACT ATAGATGTGA TACTGATCAA TGGGCGTTTG 
AAGGAAGGAG ATACAATTAT TGTTCCTGGA GTAGAAGGGC CCATTGTAAC TCAGATTCGA 
GGCCTCCTAT TACCTCCTCC TATGAAGGAA TTAAGGGTGA AGAACCAGTA TGAAAAGCAT 
AAAGAAGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AAGACCTAGA GAAGACATTG 
GCTGGTTTAC CCCTCCTGGT GGCTTATAAA GAAGATGAAA TCCCAGTTCT TAAAGATGAG 
TTGATCCATG AGTTAAAGCA GACGCTAAAT GCTATCAAGT TGGAAGAAAA AGGAGTCTAT 
GTTCAAGCAT CTACACTGGG ATCTTTGGAA GCTCTACTTG AATTTCTAAA AACATCAGAA 
GTGCCCTATG CAGGAATTAA CATTGGTCCA GTCCATAAAA AAGATGTTAT GAAGGCTTCA 
GTGATGCTAG AACATGATCC CCAGTATGCA GTAATTTTGG CCTTTGATGT GAGAATTGAA 
CGGGATGCAC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAT AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ATATAGCAGT ATTTCCCTGC AAGATGAAAA TCCTCCCCCA GTATATTTTT 
AATTCTCGAG ACCCAATAGT GATGGGAGTG ACTGTGGAAG CAGGTCAGGT GAAACAGGGG 
ACACCCATGT GCGTCCCTAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
ATAAACCATA AACAAGTGGA TGTCGCAAAA AAAGGACAAG AAGTCTGTGT CAAAATAGAA 
CCTATCCCTG GTGAATCTCC CAAAATGTAT GGAAGACATT TTGAAGCTAC AGACATTCTT 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCCCTCAAGG ACTGGTTCAG AGATGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TATTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_005626022.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626079.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

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CloneID	OCe13662
Clone ID Related Accession (Same CDS sequence)	XM_005626022.3 , XM_005626022.2
Accession Version	XM_005626022.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	eukaryotic translation initiation factor 5B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAAATACT 
GTGCATATAG AAGTAAAAGA AAACCCTGAG GAGGAAGAGG AAGAGGAGGA AGAAGAGAGT 
GAAGAAGAAG AGAGTGAAGA GGAAGAGGAA GAGGAAGGAG AAAGTGAAGG CAGTGAAGGT 
GATGAAGAAG ATGAAAAGGT GTCCGATGAG AAGGATGCAG GGAAAATGTT AGATAAAAAA 
CCAAGTAAAG AAATGAGTTC AGAATCTGAA TACGACTCTG ATGATGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC CAAACGGAGG ATTGAGAAAC GGCGACTTGA ACATAGTAAA 
AATGTAAATA CAGAAAAGCT AAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGACACA 
GGGAAGACAA AGATTCTAGA TAAACTTCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGCATTACTC AACAAATTGG GGCCACCAAT GTTCCCCTTG AAGCTATTAA TGAACAGACT 
AAGATGATTA AAAATTTTGA TAGAGAGAAC GTACGAATTC CAGGAATGCT CATTATTGAT 
ACTCCTGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGATATT 
GCTATTTTAG TTGTTGACAT TATGCATGGT TTGGAGCCTC AGACAATTGA ATCTATTAAT 
CTTCTGAAAT CTAAAAAATG CCCCTTCATT GTTGCACTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGATGTG GCTGCTACTT TAAAGAAGCA GAAAAAGAAT 
ACAAAAGATG AATTTGAGGA GCGAGCAAAG GCTATTATTG TGGAATTTGC ACAGCAGGGT 
TTGAATGCAG CTTTGTTTTA TGAGAATAAG GATCCCCGTA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCGC ATACTGGTGA TGGTATGGGA AGTCTGATCT ACCTTCTTGT TGAGTTAACT 
CAGACCATGT TAAGCAAGAG ACTTGCACAC TGTGAGGAGC TAAGAGCACA GGTTATGGAG 
GTTAAGGCTC TACCAGGAAT GGGCACCACT ATAGATGTGA TACTGATCAA TGGGCGTTTG 
AAGGAAGGAG ATACAATTAT TGTTCCTGGA GTAGAAGGGC CCATTGTAAC TCAGATTCGA 
GGCCTCCTAT TACCTCCTCC TATGAAGGAA TTAAGGGTGA AGAACCAGTA TGAAAAGCAT 
AAAGAAGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AAGACCTAGA GAAGACATTG 
GCTGGTTTAC CCCTCCTGGT GGCTTATAAA GAAGATGAAA TCCCAGTTCT TAAAGATGAG 
TTGATCCATG AGTTAAAGCA GACGCTAAAT GCTATCAAGT TGGAAGAAAA AGGAGTCTAT 
GTTCAAGCAT CTACACTGGG ATCTTTGGAA GCTCTACTTG AATTTCTAAA AACATCAGAA 
GTGCCCTATG CAGGAATTAA CATTGGTCCA GTCCATAAAA AAGATGTTAT GAAGGCTTCA 
GTGATGCTAG AACATGATCC CCAGTATGCA GTAATTTTGG CCTTTGATGT GAGAATTGAA 
CGGGATGCAC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAT AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ATATAGCAGT ATTTCCCTGC AAGATGAAAA TCCTCCCCCA GTATATTTTT 
AATTCTCGAG ACCCAATAGT GATGGGAGTG ACTGTGGAAG CAGGTCAGGT GAAACAGGGG 
ACACCCATGT GCGTCCCTAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
ATAAACCATA AACAAGTGGA TGTCGCAAAA AAAGGACAAG AAGTCTGTGT CAAAATAGAA 
CCTATCCCTG GTGAATCTCC CAAAATGTAT GGAAGACATT TTGAAGCTAC AGACATTCTT 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCCCTCAAGG ACTGGTTCAG AGATGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TATTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626079.1
CDS	169..3825
Translation	MGKKQKNKSEDSAKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADRESAAVKPTENNEEESTSKQDKKKKGQKGKKQSFDDNDSEEWEDKDSKSKKTAKSKVEMYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626022.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626022.2

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAGCGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAGG AACAGGAACC TCAAAAATCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGG CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA AAGTGCTGCA 
GTAAAGCCAA CAGAAAATAA TGAAGAAGAG TCCACCTCAA AACAAGATAA AAAAAAGAAA 
GGACAAAAAG GCAAAAAACA AAGTTTTGAT GATAATGATA GTGAAGAATG GGAAGATAAA 
GATTCAAAAT CAAAAAAGAC TGCAAAGTCA AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATG ATTTTAATAA ACTTTCTAAA AAAGCTAAAG GGAAAGTGCA GAAATCAAAT 
AAAAGGGGGG ATGGGTCAGA AGAAGATGAG GATAACAGTA AAAGAGTTAA AGAGCGTTCC 
CGAGTAAATT CTTCAGGTGA AAGTGGTGAT GAATCAGATG AATTTTTACA GTCCAGAAAA 
GGACAGAAAA AAAATCAAAA AAATAAGCCA AGTCCTAATG TGGAGAGTGG GAATGAAGAT 
GATGATTCCT CCTTCAAAAT TAAGACGGTG GCCCAAAAGA AGGCAGAAAA GAAAGAGCGT 
GAAAGAAAAA AGAGAGATGA AGAAAAAGCA AAACTACGGA AGCTGAAAGA AAAAGAAGAG 
CTAGAGACTG GGAAAAAGGA TCAGAGTAAA CAATCTCAAA AGAAATCTGA AGAAGAAGCT 
GTAAAATCCA AAGTGACTCT TGATACCGGA GCAGTCCCTG CCTCTGAAGA GAAAGGAGAG 
ACTCCCACAG CTGCTGAAGA TGACAATGAA GGAGACAAAA AGAAAAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCAACA 
GTAAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAAG AAGAAGAACG GCAGAAGAGA 
GAAGAGGAGG AACGCATAAA ACGACTTGAA GAGTTAGAAG CCAAGCGTAA AGAAGAGGAA 
AGACTAGAAC AAGAAAAGAG AGAAAGGAAA AAGCAAAAAG AGAAAGAAAG AAAAGAACGC 
TTAAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCTGAA 
GCTACCCTTA AACTTCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAAAGGC CAATTTATGA AGATAAAAAG AAGAAAAAAA CATTACAGCA GCTAGAAAAT 
AAAGAAGTAT CTGAACCAAT AGAATTAAGT GCTGCTGTAG AAGTTGTGGA ACAAGGAGTT 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AGGAAGAAGA AACTGAAGAT 
ACTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAAG AGAGAGAAAA AGAAAATACT 
GTGCATATAG AAGTAAAAGA AAACCCTGAG GAGGAAGAGG AAGAGGAGGA AGAAGAGAGT 
GAAGAAGAAG AGAGTGAAGA GGAAGAGGAA GAGGAAGGAG AAAGTGAAGG CAGTGAAGGT 
GATGAAGAAG ATGAAAAGGT GTCCGATGAG AAGGATGCAG GGAAAATGTT AGATAAAAAA 
CCAAGTAAAG AAATGAGTTC AGAATCTGAA TACGACTCTG ATGATGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC CAAACGGAGG ATTGAGAAAC GGCGACTTGA ACATAGTAAA 
AATGTAAATA CAGAAAAGCT AAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGACACA 
GGGAAGACAA AGATTCTAGA TAAACTTCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGCATTACTC AACAAATTGG GGCCACCAAT GTTCCCCTTG AAGCTATTAA TGAACAGACT 
AAGATGATTA AAAATTTTGA TAGAGAGAAC GTACGAATTC CAGGAATGCT CATTATTGAT 
ACTCCTGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGATATT 
GCTATTTTAG TTGTTGACAT TATGCATGGT TTGGAGCCTC AGACAATTGA ATCTATTAAT 
CTTCTGAAAT CTAAAAAATG CCCCTTCATT GTTGCACTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGATGTG GCTGCTACTT TAAAGAAGCA GAAAAAGAAT 
ACAAAAGATG AATTTGAGGA GCGAGCAAAG GCTATTATTG TGGAATTTGC ACAGCAGGGT 
TTGAATGCAG CTTTGTTTTA TGAGAATAAG GATCCCCGTA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCGC ATACTGGTGA TGGTATGGGA AGTCTGATCT ACCTTCTTGT TGAGTTAACT 
CAGACCATGT TAAGCAAGAG ACTTGCACAC TGTGAGGAGC TAAGAGCACA GGTTATGGAG 
GTTAAGGCTC TACCAGGAAT GGGCACCACT ATAGATGTGA TACTGATCAA TGGGCGTTTG 
AAGGAAGGAG ATACAATTAT TGTTCCTGGA GTAGAAGGGC CCATTGTAAC TCAGATTCGA 
GGCCTCCTAT TACCTCCTCC TATGAAGGAA TTAAGGGTGA AGAACCAGTA TGAAAAGCAT 
AAAGAAGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AAGACCTAGA GAAGACATTG 
GCTGGTTTAC CCCTCCTGGT GGCTTATAAA GAAGATGAAA TCCCAGTTCT TAAAGATGAG 
TTGATCCATG AGTTAAAGCA GACGCTAAAT GCTATCAAGT TGGAAGAAAA AGGAGTCTAT 
GTTCAAGCAT CTACACTGGG ATCTTTGGAA GCTCTACTTG AATTTCTAAA AACATCAGAA 
GTGCCCTATG CAGGAATTAA CATTGGTCCA GTCCATAAAA AAGATGTTAT GAAGGCTTCA 
GTGATGCTAG AACATGATCC CCAGTATGCA GTAATTTTGG CCTTTGATGT GAGAATTGAA 
CGGGATGCAC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAT AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ATATAGCAGT ATTTCCCTGC AAGATGAAAA TCCTCCCCCA GTATATTTTT 
AATTCTCGAG ACCCAATAGT GATGGGAGTG ACTGTGGAAG CAGGTCAGGT GAAACAGGGG 
ACACCCATGT GCGTCCCTAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
ATAAACCATA AACAAGTGGA TGTCGCAAAA AAAGGACAAG AAGTCTGTGT CAAAATAGAA 
CCTATCCCTG GTGAATCTCC CAAAATGTAT GGAAGACATT TTGAAGCTAC AGACATTCTT 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCCCTCAAGG ACTGGTTCAG AGATGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TATTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_005626023.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626080.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe13663
Clone ID Related Accession (Same CDS sequence)	XM_005626023.2 , XM_005626023.3
Accession Version	XM_005626023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	eukaryotic translation initiation factor 5B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG GGAGTGATGA TGATGATGAT GATTTTAATA AACTTTCTAA AAAAGCTAAA 
GGGAAAGTGC AGAAATCAAA TAAAAGGGGG GATGGGTCAG AAGAAGATGA GGATAACAGT 
AAAAGAGTTA AAGAGCGTTC CCGAGTAAAT TCTTCAGGTG AAAGTGGTGA TGAATCAGAT 
GAATTTTTAC AGTCCAGAAA AGGACAGAAA AAAAATCAAA AAAATAAGCC AAGTCCTAAT 
GTGGAGAGTG GGAATGAAGA TGATGATTCC TCCTTCAAAA TTAAGACGGT GGCCCAAAAG 
AAGGCAGAAA AGAAAGAGCG TGAAAGAAAA AAGAGAGATG AAGAAAAAGC AAAACTACGG 
AAGCTGAAAG AAAAAGAAGA GCTAGAGACT GGGAAAAAGG ATCAGAGTAA ACAATCTCAA 
AAGAAATCTG AAGAAGAAGC TGTAAAATCC AAAGTGACTC TTGATACCGG AGCAGTCCCT 
GCCTCTGAAG AGAAAGGAGA GACTCCCACA GCTGCTGAAG ATGACAATGA AGGAGACAAA 
AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA GAAAAGGAAG AAAAAGAGAA AGAGAAGAAA 
AAAGGACCTA GCAAAGCAAC AGTAAAAGCT ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA 
GAAGAAGAAC GGCAGAAGAG AGAAGAGGAG GAACGCATAA AACGACTTGA AGAGTTAGAA 
GCCAAGCGTA AAGAAGAGGA AAGACTAGAA CAAGAAAAGA GAGAAAGGAA AAAGCAAAAA 
GAGAAAGAAA GAAAAGAACG CTTAAAAAAA GAAGGGAAAC TTTTAACTAA ATCCCAGAGA 
GAAGCCAGAG CCAGAGCTGA AGCTACCCTT AAACTTCTAC AAGCTCAGGG TGTTGAAGTG 
CCATCAAAAG ACTCTTTGCC AAAGAAAAGG CCAATTTATG AAGATAAAAA GAAGAAAAAA 
ACATTACAGC AGCTAGAAAA TAAAGAAGTA TCTGAACCAA TAGAATTAAG TGCTGCTGTA 
GAAGTTGTGG AACAAGGAGT TCCAGAAAAG GAAGAGACAC CACCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACTGAAGA TACTGGATTG GATGATTGGG AAGCTATGGC CAGTGATGAA 
GAGAGAGAAA AAGAAGAAAA TACTGTGCAT ATAGAAGTAA AAGAAAACCC TGAGGAGGAA 
GAGGAAGAGG AGGAAGAAGA GAGTGAAGAA GAAGAGAGTG AAGAGGAAGA GGAAGAGGAA 
GGAGAAAGTG AAGGCAGTGA AGGTGATGAA GAAGATGAAA AGGTGTCCGA TGAGAAGGAT 
GCAGGGAAAA TGTTAGATAA AAAACCAAGT AAAGAAATGA GTTCAGAATC TGAATACGAC 
TCTGATGATG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCCAAACG GAGGATTGAG 
AAACGGCGAC TTGAACATAG TAAAAATGTA AATACAGAAA AGCTAAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA CACAGGGAAG ACAAAGATTC TAGATAAACT TCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGCATT ACTCAACAAA TTGGGGCCAC CAATGTTCCC 
CTTGAAGCTA TTAATGAACA GACTAAGATG ATTAAAAATT TTGATAGAGA GAACGTACGA 
ATTCCAGGAA TGCTCATTAT TGATACTCCT GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA TATTGCTATT TTAGTTGTTG ACATTATGCA TGGTTTGGAG 
CCTCAGACAA TTGAATCTAT TAATCTTCTG AAATCTAAAA AATGCCCCTT CATTGTTGCA 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA TGTGGCTGCT 
ACTTTAAAGA AGCAGAAAAA GAATACAAAA GATGAATTTG AGGAGCGAGC AAAGGCTATT 
ATTGTGGAAT TTGCACAGCA GGGTTTGAAT GCAGCTTTGT TTTATGAGAA TAAGGATCCC 
CGTACTTTTG TGTCCTTGGT ACCTACCTCT GCGCATACTG GTGATGGTAT GGGAAGTCTG 
ATCTACCTTC TTGTTGAGTT AACTCAGACC ATGTTAAGCA AGAGACTTGC ACACTGTGAG 
GAGCTAAGAG CACAGGTTAT GGAGGTTAAG GCTCTACCAG GAATGGGCAC CACTATAGAT 
GTGATACTGA TCAATGGGCG TTTGAAGGAA GGAGATACAA TTATTGTTCC TGGAGTAGAA 
GGGCCCATTG TAACTCAGAT TCGAGGCCTC CTATTACCTC CTCCTATGAA GGAATTAAGG 
GTGAAGAACC AGTATGAAAA GCATAAAGAA GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAAGACC TAGAGAAGAC ATTGGCTGGT TTACCCCTCC TGGTGGCTTA TAAAGAAGAT 
GAAATCCCAG TTCTTAAAGA TGAGTTGATC CATGAGTTAA AGCAGACGCT AAATGCTATC 
AAGTTGGAAG AAAAAGGAGT CTATGTTCAA GCATCTACAC TGGGATCTTT GGAAGCTCTA 
CTTGAATTTC TAAAAACATC AGAAGTGCCC TATGCAGGAA TTAACATTGG TCCAGTCCAT 
AAAAAAGATG TTATGAAGGC TTCAGTGATG CTAGAACATG ATCCCCAGTA TGCAGTAATT 
TTGGCCTTTG ATGTGAGAAT TGAACGGGAT GCACAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATATAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCATATAG CAGTATTTCC CTGCAAGATG 
AAAATCCTCC CCCAGTATAT TTTTAATTCT CGAGACCCAA TAGTGATGGG AGTGACTGTG 
GAAGCAGGTC AGGTGAAACA GGGGACACCC ATGTGCGTCC CTAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAATAAAC CATAAACAAG TGGATGTCGC AAAAAAAGGA 
CAAGAAGTCT GTGTCAAAAT AGAACCTATC CCTGGTGAAT CTCCCAAAAT GTATGGAAGA 
CATTTTGAAG CTACAGACAT TCTTGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCCCTC 
AAGGACTGGT TCAGAGATGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626080.1
CDS	400..3660
Translation	MYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKEENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626023.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626023.2

ORF Insert Sequence:

ATGTACTCTG GGAGTGATGA TGATGATGAT GATTTTAATA AACTTTCTAA AAAAGCTAAA 
GGGAAAGTGC AGAAATCAAA TAAAAGGGGG GATGGGTCAG AAGAAGATGA GGATAACAGT 
AAAAGAGTTA AAGAGCGTTC CCGAGTAAAT TCTTCAGGTG AAAGTGGTGA TGAATCAGAT 
GAATTTTTAC AGTCCAGAAA AGGACAGAAA AAAAATCAAA AAAATAAGCC AAGTCCTAAT 
GTGGAGAGTG GGAATGAAGA TGATGATTCC TCCTTCAAAA TTAAGACGGT GGCCCAAAAG 
AAGGCAGAAA AGAAAGAGCG TGAAAGAAAA AAGAGAGATG AAGAAAAAGC AAAACTACGG 
AAGCTGAAAG AAAAAGAAGA GCTAGAGACT GGGAAAAAGG ATCAGAGTAA ACAATCTCAA 
AAGAAATCTG AAGAAGAAGC TGTAAAATCC AAAGTGACTC TTGATACCGG AGCAGTCCCT 
GCCTCTGAAG AGAAAGGAGA GACTCCCACA GCTGCTGAAG ATGACAATGA AGGAGACAAA 
AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA GAAAAGGAAG AAAAAGAGAA AGAGAAGAAA 
AAAGGACCTA GCAAAGCAAC AGTAAAAGCT ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA 
GAAGAAGAAC GGCAGAAGAG AGAAGAGGAG GAACGCATAA AACGACTTGA AGAGTTAGAA 
GCCAAGCGTA AAGAAGAGGA AAGACTAGAA CAAGAAAAGA GAGAAAGGAA AAAGCAAAAA 
GAGAAAGAAA GAAAAGAACG CTTAAAAAAA GAAGGGAAAC TTTTAACTAA ATCCCAGAGA 
GAAGCCAGAG CCAGAGCTGA AGCTACCCTT AAACTTCTAC AAGCTCAGGG TGTTGAAGTG 
CCATCAAAAG ACTCTTTGCC AAAGAAAAGG CCAATTTATG AAGATAAAAA GAAGAAAAAA 
ACATTACAGC AGCTAGAAAA TAAAGAAGTA TCTGAACCAA TAGAATTAAG TGCTGCTGTA 
GAAGTTGTGG AACAAGGAGT TCCAGAAAAG GAAGAGACAC CACCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACTGAAGA TACTGGATTG GATGATTGGG AAGCTATGGC CAGTGATGAA 
GAGAGAGAAA AAGAAGAAAA TACTGTGCAT ATAGAAGTAA AAGAAAACCC TGAGGAGGAA 
GAGGAAGAGG AGGAAGAAGA GAGTGAAGAA GAAGAGAGTG AAGAGGAAGA GGAAGAGGAA 
GGAGAAAGTG AAGGCAGTGA AGGTGATGAA GAAGATGAAA AGGTGTCCGA TGAGAAGGAT 
GCAGGGAAAA TGTTAGATAA AAAACCAAGT AAAGAAATGA GTTCAGAATC TGAATACGAC 
TCTGATGATG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCCAAACG GAGGATTGAG 
AAACGGCGAC TTGAACATAG TAAAAATGTA AATACAGAAA AGCTAAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA CACAGGGAAG ACAAAGATTC TAGATAAACT TCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGCATT ACTCAACAAA TTGGGGCCAC CAATGTTCCC 
CTTGAAGCTA TTAATGAACA GACTAAGATG ATTAAAAATT TTGATAGAGA GAACGTACGA 
ATTCCAGGAA TGCTCATTAT TGATACTCCT GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA TATTGCTATT TTAGTTGTTG ACATTATGCA TGGTTTGGAG 
CCTCAGACAA TTGAATCTAT TAATCTTCTG AAATCTAAAA AATGCCCCTT CATTGTTGCA 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA TGTGGCTGCT 
ACTTTAAAGA AGCAGAAAAA GAATACAAAA GATGAATTTG AGGAGCGAGC AAAGGCTATT 
ATTGTGGAAT TTGCACAGCA GGGTTTGAAT GCAGCTTTGT TTTATGAGAA TAAGGATCCC 
CGTACTTTTG TGTCCTTGGT ACCTACCTCT GCGCATACTG GTGATGGTAT GGGAAGTCTG 
ATCTACCTTC TTGTTGAGTT AACTCAGACC ATGTTAAGCA AGAGACTTGC ACACTGTGAG 
GAGCTAAGAG CACAGGTTAT GGAGGTTAAG GCTCTACCAG GAATGGGCAC CACTATAGAT 
GTGATACTGA TCAATGGGCG TTTGAAGGAA GGAGATACAA TTATTGTTCC TGGAGTAGAA 
GGGCCCATTG TAACTCAGAT TCGAGGCCTC CTATTACCTC CTCCTATGAA GGAATTAAGG 
GTGAAGAACC AGTATGAAAA GCATAAAGAA GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAAGACC TAGAGAAGAC ATTGGCTGGT TTACCCCTCC TGGTGGCTTA TAAAGAAGAT 
GAAATCCCAG TTCTTAAAGA TGAGTTGATC CATGAGTTAA AGCAGACGCT AAATGCTATC 
AAGTTGGAAG AAAAAGGAGT CTATGTTCAA GCATCTACAC TGGGATCTTT GGAAGCTCTA 
CTTGAATTTC TAAAAACATC AGAAGTGCCC TATGCAGGAA TTAACATTGG TCCAGTCCAT 
AAAAAAGATG TTATGAAGGC TTCAGTGATG CTAGAACATG ATCCCCAGTA TGCAGTAATT 
TTGGCCTTTG ATGTGAGAAT TGAACGGGAT GCACAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATATAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCATATAG CAGTATTTCC CTGCAAGATG 
AAAATCCTCC CCCAGTATAT TTTTAATTCT CGAGACCCAA TAGTGATGGG AGTGACTGTG 
GAAGCAGGTC AGGTGAAACA GGGGACACCC ATGTGCGTCC CTAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAATAAAC CATAAACAAG TGGATGTCGC AAAAAAAGGA 
CAAGAAGTCT GTGTCAAAAT AGAACCTATC CCTGGTGAAT CTCCCAAAAT GTATGGAAGA 
CATTTTGAAG CTACAGACAT TCTTGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCCCTC 
AAGGACTGGT TCAGAGATGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_005626023.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626080.1 Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

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CloneID	OCe13663
Clone ID Related Accession (Same CDS sequence)	XM_005626023.2 , XM_005626023.3
Accession Version	XM_005626023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	eukaryotic translation initiation factor 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG GGAGTGATGA TGATGATGAT GATTTTAATA AACTTTCTAA AAAAGCTAAA 
GGGAAAGTGC AGAAATCAAA TAAAAGGGGG GATGGGTCAG AAGAAGATGA GGATAACAGT 
AAAAGAGTTA AAGAGCGTTC CCGAGTAAAT TCTTCAGGTG AAAGTGGTGA TGAATCAGAT 
GAATTTTTAC AGTCCAGAAA AGGACAGAAA AAAAATCAAA AAAATAAGCC AAGTCCTAAT 
GTGGAGAGTG GGAATGAAGA TGATGATTCC TCCTTCAAAA TTAAGACGGT GGCCCAAAAG 
AAGGCAGAAA AGAAAGAGCG TGAAAGAAAA AAGAGAGATG AAGAAAAAGC AAAACTACGG 
AAGCTGAAAG AAAAAGAAGA GCTAGAGACT GGGAAAAAGG ATCAGAGTAA ACAATCTCAA 
AAGAAATCTG AAGAAGAAGC TGTAAAATCC AAAGTGACTC TTGATACCGG AGCAGTCCCT 
GCCTCTGAAG AGAAAGGAGA GACTCCCACA GCTGCTGAAG ATGACAATGA AGGAGACAAA 
AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA GAAAAGGAAG AAAAAGAGAA AGAGAAGAAA 
AAAGGACCTA GCAAAGCAAC AGTAAAAGCT ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA 
GAAGAAGAAC GGCAGAAGAG AGAAGAGGAG GAACGCATAA AACGACTTGA AGAGTTAGAA 
GCCAAGCGTA AAGAAGAGGA AAGACTAGAA CAAGAAAAGA GAGAAAGGAA AAAGCAAAAA 
GAGAAAGAAA GAAAAGAACG CTTAAAAAAA GAAGGGAAAC TTTTAACTAA ATCCCAGAGA 
GAAGCCAGAG CCAGAGCTGA AGCTACCCTT AAACTTCTAC AAGCTCAGGG TGTTGAAGTG 
CCATCAAAAG ACTCTTTGCC AAAGAAAAGG CCAATTTATG AAGATAAAAA GAAGAAAAAA 
ACATTACAGC AGCTAGAAAA TAAAGAAGTA TCTGAACCAA TAGAATTAAG TGCTGCTGTA 
GAAGTTGTGG AACAAGGAGT TCCAGAAAAG GAAGAGACAC CACCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACTGAAGA TACTGGATTG GATGATTGGG AAGCTATGGC CAGTGATGAA 
GAGAGAGAAA AAGAAGAAAA TACTGTGCAT ATAGAAGTAA AAGAAAACCC TGAGGAGGAA 
GAGGAAGAGG AGGAAGAAGA GAGTGAAGAA GAAGAGAGTG AAGAGGAAGA GGAAGAGGAA 
GGAGAAAGTG AAGGCAGTGA AGGTGATGAA GAAGATGAAA AGGTGTCCGA TGAGAAGGAT 
GCAGGGAAAA TGTTAGATAA AAAACCAAGT AAAGAAATGA GTTCAGAATC TGAATACGAC 
TCTGATGATG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCCAAACG GAGGATTGAG 
AAACGGCGAC TTGAACATAG TAAAAATGTA AATACAGAAA AGCTAAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA CACAGGGAAG ACAAAGATTC TAGATAAACT TCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGCATT ACTCAACAAA TTGGGGCCAC CAATGTTCCC 
CTTGAAGCTA TTAATGAACA GACTAAGATG ATTAAAAATT TTGATAGAGA GAACGTACGA 
ATTCCAGGAA TGCTCATTAT TGATACTCCT GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA TATTGCTATT TTAGTTGTTG ACATTATGCA TGGTTTGGAG 
CCTCAGACAA TTGAATCTAT TAATCTTCTG AAATCTAAAA AATGCCCCTT CATTGTTGCA 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA TGTGGCTGCT 
ACTTTAAAGA AGCAGAAAAA GAATACAAAA GATGAATTTG AGGAGCGAGC AAAGGCTATT 
ATTGTGGAAT TTGCACAGCA GGGTTTGAAT GCAGCTTTGT TTTATGAGAA TAAGGATCCC 
CGTACTTTTG TGTCCTTGGT ACCTACCTCT GCGCATACTG GTGATGGTAT GGGAAGTCTG 
ATCTACCTTC TTGTTGAGTT AACTCAGACC ATGTTAAGCA AGAGACTTGC ACACTGTGAG 
GAGCTAAGAG CACAGGTTAT GGAGGTTAAG GCTCTACCAG GAATGGGCAC CACTATAGAT 
GTGATACTGA TCAATGGGCG TTTGAAGGAA GGAGATACAA TTATTGTTCC TGGAGTAGAA 
GGGCCCATTG TAACTCAGAT TCGAGGCCTC CTATTACCTC CTCCTATGAA GGAATTAAGG 
GTGAAGAACC AGTATGAAAA GCATAAAGAA GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAAGACC TAGAGAAGAC ATTGGCTGGT TTACCCCTCC TGGTGGCTTA TAAAGAAGAT 
GAAATCCCAG TTCTTAAAGA TGAGTTGATC CATGAGTTAA AGCAGACGCT AAATGCTATC 
AAGTTGGAAG AAAAAGGAGT CTATGTTCAA GCATCTACAC TGGGATCTTT GGAAGCTCTA 
CTTGAATTTC TAAAAACATC AGAAGTGCCC TATGCAGGAA TTAACATTGG TCCAGTCCAT 
AAAAAAGATG TTATGAAGGC TTCAGTGATG CTAGAACATG ATCCCCAGTA TGCAGTAATT 
TTGGCCTTTG ATGTGAGAAT TGAACGGGAT GCACAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATATAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCATATAG CAGTATTTCC CTGCAAGATG 
AAAATCCTCC CCCAGTATAT TTTTAATTCT CGAGACCCAA TAGTGATGGG AGTGACTGTG 
GAAGCAGGTC AGGTGAAACA GGGGACACCC ATGTGCGTCC CTAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAATAAAC CATAAACAAG TGGATGTCGC AAAAAAAGGA 
CAAGAAGTCT GTGTCAAAAT AGAACCTATC CCTGGTGAAT CTCCCAAAAT GTATGGAAGA 
CATTTTGAAG CTACAGACAT TCTTGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCCCTC 
AAGGACTGGT TCAGAGATGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626080.1
CDS	390..3650
Translation	MYSGSDDDDDDFNKLSKKAKGKVQKSNKRGDGSEEDEDNSKRVKERSRVNSSGESGDESDEFLQSRKGQKKNQKNKPSPNVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQSQKKSEEEAVKSKVTLDTGAVPASEEKGETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKTLQQLENKEVSEPIELSAAVEVVEQGVPEKEETPPPVEPEEEEETEDTGLDDWEAMASDEEREKEENTVHIEVKENPEEEEEEEEEESEEEESEEEEEEEGESEGSEGDEEDEKVSDEKDAGKMLDKKPSKEMSSESEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_005626023.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626023.3

ORF Insert Sequence:

ATGTACTCTG GGAGTGATGA TGATGATGAT GATTTTAATA AACTTTCTAA AAAAGCTAAA 
GGGAAAGTGC AGAAATCAAA TAAAAGGGGG GATGGGTCAG AAGAAGATGA GGATAACAGT 
AAAAGAGTTA AAGAGCGTTC CCGAGTAAAT TCTTCAGGTG AAAGTGGTGA TGAATCAGAT 
GAATTTTTAC AGTCCAGAAA AGGACAGAAA AAAAATCAAA AAAATAAGCC AAGTCCTAAT 
GTGGAGAGTG GGAATGAAGA TGATGATTCC TCCTTCAAAA TTAAGACGGT GGCCCAAAAG 
AAGGCAGAAA AGAAAGAGCG TGAAAGAAAA AAGAGAGATG AAGAAAAAGC AAAACTACGG 
AAGCTGAAAG AAAAAGAAGA GCTAGAGACT GGGAAAAAGG ATCAGAGTAA ACAATCTCAA 
AAGAAATCTG AAGAAGAAGC TGTAAAATCC AAAGTGACTC TTGATACCGG AGCAGTCCCT 
GCCTCTGAAG AGAAAGGAGA GACTCCCACA GCTGCTGAAG ATGACAATGA AGGAGACAAA 
AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA GAAAAGGAAG AAAAAGAGAA AGAGAAGAAA 
AAAGGACCTA GCAAAGCAAC AGTAAAAGCT ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA 
GAAGAAGAAC GGCAGAAGAG AGAAGAGGAG GAACGCATAA AACGACTTGA AGAGTTAGAA 
GCCAAGCGTA AAGAAGAGGA AAGACTAGAA CAAGAAAAGA GAGAAAGGAA AAAGCAAAAA 
GAGAAAGAAA GAAAAGAACG CTTAAAAAAA GAAGGGAAAC TTTTAACTAA ATCCCAGAGA 
GAAGCCAGAG CCAGAGCTGA AGCTACCCTT AAACTTCTAC AAGCTCAGGG TGTTGAAGTG 
CCATCAAAAG ACTCTTTGCC AAAGAAAAGG CCAATTTATG AAGATAAAAA GAAGAAAAAA 
ACATTACAGC AGCTAGAAAA TAAAGAAGTA TCTGAACCAA TAGAATTAAG TGCTGCTGTA 
GAAGTTGTGG AACAAGGAGT TCCAGAAAAG GAAGAGACAC CACCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACTGAAGA TACTGGATTG GATGATTGGG AAGCTATGGC CAGTGATGAA 
GAGAGAGAAA AAGAAGAAAA TACTGTGCAT ATAGAAGTAA AAGAAAACCC TGAGGAGGAA 
GAGGAAGAGG AGGAAGAAGA GAGTGAAGAA GAAGAGAGTG AAGAGGAAGA GGAAGAGGAA 
GGAGAAAGTG AAGGCAGTGA AGGTGATGAA GAAGATGAAA AGGTGTCCGA TGAGAAGGAT 
GCAGGGAAAA TGTTAGATAA AAAACCAAGT AAAGAAATGA GTTCAGAATC TGAATACGAC 
TCTGATGATG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCCAAACG GAGGATTGAG 
AAACGGCGAC TTGAACATAG TAAAAATGTA AATACAGAAA AGCTAAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA CACAGGGAAG ACAAAGATTC TAGATAAACT TCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGCATT ACTCAACAAA TTGGGGCCAC CAATGTTCCC 
CTTGAAGCTA TTAATGAACA GACTAAGATG ATTAAAAATT TTGATAGAGA GAACGTACGA 
ATTCCAGGAA TGCTCATTAT TGATACTCCT GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA TATTGCTATT TTAGTTGTTG ACATTATGCA TGGTTTGGAG 
CCTCAGACAA TTGAATCTAT TAATCTTCTG AAATCTAAAA AATGCCCCTT CATTGTTGCA 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA TGTGGCTGCT 
ACTTTAAAGA AGCAGAAAAA GAATACAAAA GATGAATTTG AGGAGCGAGC AAAGGCTATT 
ATTGTGGAAT TTGCACAGCA GGGTTTGAAT GCAGCTTTGT TTTATGAGAA TAAGGATCCC 
CGTACTTTTG TGTCCTTGGT ACCTACCTCT GCGCATACTG GTGATGGTAT GGGAAGTCTG 
ATCTACCTTC TTGTTGAGTT AACTCAGACC ATGTTAAGCA AGAGACTTGC ACACTGTGAG 
GAGCTAAGAG CACAGGTTAT GGAGGTTAAG GCTCTACCAG GAATGGGCAC CACTATAGAT 
GTGATACTGA TCAATGGGCG TTTGAAGGAA GGAGATACAA TTATTGTTCC TGGAGTAGAA 
GGGCCCATTG TAACTCAGAT TCGAGGCCTC CTATTACCTC CTCCTATGAA GGAATTAAGG 
GTGAAGAACC AGTATGAAAA GCATAAAGAA GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAAGACC TAGAGAAGAC ATTGGCTGGT TTACCCCTCC TGGTGGCTTA TAAAGAAGAT 
GAAATCCCAG TTCTTAAAGA TGAGTTGATC CATGAGTTAA AGCAGACGCT AAATGCTATC 
AAGTTGGAAG AAAAAGGAGT CTATGTTCAA GCATCTACAC TGGGATCTTT GGAAGCTCTA 
CTTGAATTTC TAAAAACATC AGAAGTGCCC TATGCAGGAA TTAACATTGG TCCAGTCCAT 
AAAAAAGATG TTATGAAGGC TTCAGTGATG CTAGAACATG ATCCCCAGTA TGCAGTAATT 
TTGGCCTTTG ATGTGAGAAT TGAACGGGAT GCACAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATATAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCATATAG CAGTATTTCC CTGCAAGATG 
AAAATCCTCC CCCAGTATAT TTTTAATTCT CGAGACCCAA TAGTGATGGG AGTGACTGTG 
GAAGCAGGTC AGGTGAAACA GGGGACACCC ATGTGCGTCC CTAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAATAAAC CATAAACAAG TGGATGTCGC AAAAAAAGGA 
CAAGAAGTCT GTGTCAAAAT AGAACCTATC CCTGGTGAAT CTCCCAAAAT GTATGGAAGA 
CATTTTGAAG CTACAGACAT TCTTGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCCCTC 
AAGGACTGGT TCAGAGATGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EIF5B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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