EIF5B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF5B
Entrez Gene ID	9669
Full Name	eukaryotic translation initiation factor 5B
Synonyms	IF2
General protein information	Preferred Names eukaryotic translation initiation factor 5B Names eukaryotic translation initiation factor 5B eIF-5B translation initiation factor IF-2 translation initiation factor IF2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Summary	Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008].
Disorder MIM:	606086

mRNA and Protein(s)

mRNA	Protein	Name
NM_015904.3	NP_056988.3	eukaryotic translation initiation factor 5B
NM_015904.4	NP_056988.3	eukaryotic translation initiation factor 5B

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF5B	NP_056988.3
Rattus norvegicus (Norway rat)	Eif5b	NP_001103611.1
Bos taurus (cattle)	EIF5B	XP_003586655.1
Gallus gallus (chicken)	EIF5B	NP_001033585.1
Pan troglodytes (chimpanzee)	EIF5B	XP_001159759.1
Canis lupus familiaris (dog)	EIF5B	XP_005626079.1
	EIF5B	XP_001103720.2
Xenopus tropicalis (tropical clawed frog)	eif5b	XP_004911863.1
Mus musculus (house mouse)	Eif5b	NP_938045.2
Danio rerio (zebrafish)	eif5b	XP_001923573.4

Gene Ontology
Bibliography

Related articles in PubMed

Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S
Cell reports22(1)17-26(2018 Jan)

eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
Wang ZG, Zheng H, Gao W, Han J, Cao JZ, Yang Y, Li S, Gao R, Liu H, Pan ZY, Fu SY, Gu FM, Xing H, Ni JS, Yan HL, Ren H, Zhou WP
Oncotarget7(38)62327-62339(2016 09)

eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling.
Nag N, Lin KY, Edmonds KA, Yu J, Nadkarni D, Marintcheva B, Marintchev A
Nucleic acids research44(15)7441-56(2016 Sep)

Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Jiang X, Jiang X, Feng Y, Xu R, Wang Q, Deng H
PloS one11(12)e0168387(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that the interactions between eIF1A and eIF5B are being continuously rearranged during translation initiation; presentation of a model how the dynamic eIF1A/eIF5B interaction network can promote remodeling of the translation initiation complexes, and the roles in the process played by intrinsically disordered protein segments
Title: eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling.

eIF5B silencing provides a negative feedback to deactivate MAPK signaling
Title: Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.

eIF5B overexpression promotes maturation of G0-like immature oocytes and causes cell death, an alternative to G0, in serum-starved THP1 cells.
Title: Upregulation of eIF5B controls cell-cycle arrest and specific developmental stages.

The authors show that the cleavage of initiation factor eIF5B during enteroviral infection, along with the viral internal ribosome entry site, plays a role in mediating viral translation under conditions that are nonpermissive for host cell translation.
Title: Poliovirus switches to an eIF2-independent mode of translation during infection.

3Cpro-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells.
Title: Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases.

The following EIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30523	NM_015904.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
View Old Accession Versions >>
OHu30523	NM_015904.3	Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF5B ( NM_015904.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056988.3 Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Price & Availability↑

CloneID	OHu30523
Clone ID Related Accession (Same CDS sequence)	NM_015904.3 , NM_015904.4
Accession Version	NM_015904.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX485839.1, AB018284.1, BC032639.1 and AC018690.5. On Dec 29, 2005 this sequence version replaced NM_015904.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018284.1, AJ006776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAAG AACAGGAGCC TCAAAAGTCA 
AAAGGGAAAA AGAAAAAAGA GAAAAAAAAG CAGGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTT GGAAGCTCAA GGCATCAAAG CTGACAGAGA AACTGTTGCA 
GTGAAGCCAA CAGAAAACAA TGAAGAGGAA TTCACCTCAA AAGATAAAAA AAAGAAAGGA 
CAGAAGGGCA AAAAACAGAG TTTTGATGAT AATGATAGCG AAGAATTGGA AGATAAAGAT 
TCAAAATCAA AAAAGACTGC AAAACCGAAA GTGGAAATGT ACTCTGGGAG TGATGATGAT 
GATGATTTTA ACAAACTTCC TAAAAAAGCT AAAGGGAAAG CTCAAAAATC AAATAAGAAG 
TGGGATGGGT CAGAGGAGGA TGAGGATAAC AGTAAAAAAA TTAAAGAGCG TTCAAGAATA 
AATTCTTCTG GTGAAAGTGG TGATGAATCA GATGAATTTT TGCAATCTAG AAAAGGACAG 
AAAAAAAATC AGAAAAACAA GCCAGGTCCT AACATAGAAA GTGGGAATGA AGATGATGAC 
GCCTCCTTCA AAATTAAGAC AGTGGCCCAA AAGAAGGCAG AAAAGAAGGA GCGCGAGAGA 
AAAAAGCGAG ATGAAGAAAA AGCGAAACTG CGGAAGCTGA AAGAAAAAGA AGAGTTAGAA 
ACAGGTAAAA AGGATCAGAG TAAACAAAAG GAATCTCAAA GGAAATTTGA AGAAGAAACT 
GTAAAATCCA AAGTGACTGT TGATACTGGA GTAATTCCTG CCTCTGAAGA GAAAGCAGAG 
ACTCCCACAG CTGCAGAAGA TGACAATGAA GGAGACAAAA AGAAGAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCCACT 
GTTAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAGG AAGAAGAAAG ACAGAAGAGA 
GAAGAGGAAG AACGTATAAA ACGGCTTGAA GAATTAGAAG CCAAGCGTAA AGAAGAGGAA 
CGATTGGAAC AAGAAAAAAG AGAAAGGAAA AAGCAAAAAG AAAAAGAAAG AAAAGAACGC 
TTGAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCCGAA 
GCTACTCTTA AACTGCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAGAGGC CAATTTATGA AGATAAAAAG AGGAAAAAAA TACCACAGCA GCTAGAAAGT 
AAAGAAGTGT CTGAATCAAT GGAATTATGT GCTGCTGTAG AAGTTATGGA ACAAGGAGTA 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AAGAAGAAGA TACTGAGGAT 
GCTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAGG AGACAGAAAA AGTAGAAGGA 
AACAAAGTTC ATATAGAAGT AAAAGAAAAC CCTGAAGAGG AGGAGGAGGA GGAAGAAGAG 
GAAGAAGAAG ATGAAGAAAG TGAAGAAGAG GAGGAAGAGG AGGGAGAAAG TGAAGGCAGT 
GAAGGTGATG AGGAAGATGA AAAGGTGTCA GATGAGAAGG ATTCAGGGAA GACATTAGAT 
AAAAAGCCAA GTAAAGAAAT GAGCTCAGAT TCTGAATATG ACTCTGATGA TGATCGGACT 
AAAGAAGAAA GGGCTTATGA CAAAGCAAAA CGGAGGATTG AGAAACGGCG ACTTGAACAT 
AGTAAAAATG TAAACACCGA AAAGCTAAGA GCCCCTATTA TCTGCGTACT TGGGCATGTG 
GACACAGGGA AGACAAAAAT TCTAGATAAG CTCCGTCACA CACATGTACA AGATGGTGAA 
GCAGGTGGTA TCACACAACA AATTGGGGCC ACCAATGTTC CTCTTGAAGC TATTAATGAA 
CAGACTAAGA TGATTAAAAA TTTTGATAGA GAGAATGTAC GGATTCCAGG AATGCTAATT 
ATTGATACTC CTGGGCATGA ATCTTTCAGT AATCTGAGAA ATAGAGGAAG CTCTCTTTGT 
GACATTGCCA TTTTAGTTGT TGATATTATG CATGGTTTGG AGCCCCAGAC AATTGAGTCT 
ATCAACCTTC TCAAATCTAA AAAATGTCCC TTCATTGTTG CACTCAATAA GATTGATAGG 
TTATATGATT GGAAAAAGAG TCCTGACTCT GATGTGGCTG CTACTTTAAA GAAGCAGAAA 
AAGAATACAA AAGATGAATT TGAGGAGCGA GCAAAGGCTA TTATTGTAGA ATTTGCACAG 
CAGGGTTTGA ATGCTGCTTT GTTTTATGAG AATAAAGATC CCCGCACTTT TGTGTCTTTG 
GTACCTACCT CTGCACATAC TGGTGATGGC ATGGGAAGTC TGATCTACCT TCTTGTAGAG 
TTAACTCAGA CCATGTTGAG CAAGAGACTT GCACACTGTG AAGAGCTGAG AGCACAGGTG 
ATGGAGGTTA AAGCTCTCCC GGGGATGGGC ACCACTATAG ATGTCATCTT GATCAATGGG 
CGTTTGAAGG AAGGAGATAC AATCATTGTT CCTGGAGTAG AAGGGCCCAT TGTAACTCAG 
ATTCGAGGCC TCCTGTTACC TCCTCCTATG AAGGAATTAC GAGTGAAGAA CCAGTATGAA 
AAGCATAAAG AAGTAGAAGC AGCTCAGGGG GTAAAGATTC TTGGAAAAGA CCTGGAGAAA 
ACATTGGCTG GTTTACCCCT CCTTGTGGCT TATAAAGAAG ATGAAATCCC TGTTCTTAAA 
GATGAATTGA TCCATGAGTT AAAGCAGACA CTAAATGCTA TCAAATTAGA AGAAAAAGGA 
GTCTATGTCC AGGCATCTAC ACTGGGTTCT TTGGAAGCTC TACTGGAATT TCTGAAAACA 
TCAGAAGTGC CCTATGCAGG AATTAACATT GGCCCAGTGC ATAAAAAAGA TGTTATGAAG 
GCTTCAGTGA TGTTGGAACA TGACCCTCAG TATGCAGTAA TTTTGGCCTT CGATGTGAGA 
ATTGAACGAG ATGCACAAGA AATGGCTGAT AGTTTAGGAG TTAGAATTTT TAGTGCAGAA 
ATTATTTATC ATTTATTTGA TGCCTTTACA AAATATAGAC AAGACTACAA GAAACAGAAA 
CAAGAAGAAT TTAAGCACAT AGCAGTATTT CCCTGCAAGA TAAAAATCCT CCCTCAGTAC 
ATTTTTAATT CTCGAGATCC GATAGTGATG GGGGTGACGG TGGAAGCAGG TCAGGTGAAA 
CAGGGGACAC CCATGTGTGT CCCAAGCAAA AATTTTGTTG ACATCGGAAT AGTAACAAGT 
ATTGAAATAA ACCATAAACA AGTGGATGTT GCAAAAAAAG GACAAGAAGT TTGTGTAAAA 
ATAGAACCTA TCCCTGGTGA GTCACCCAAA ATGTTTGGAA GACATTTTGA AGCTACAGAT 
ATTCTTGTTA GTAAGATCAG CCGGCAGTCC ATTGATGCAC TCAAAGACTG GTTCAGAGAT 
GAAATGCAGA AGAGTGACTG GCAGCTTATT GTGGAGCTGA AGAAAGTATT TGAAATCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056988.3
CDS	185..3847
Translation	MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	NM_015904.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015904.3

ORF Insert Sequence:

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAAG AACAGGAGCC TCAAAAGTCA 
AAAGGGAAAA AGAAAAAAGA GAAAAAAAAG CAGGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTT GGAAGCTCAA GGCATCAAAG CTGACAGAGA AACTGTTGCA 
GTGAAGCCAA CAGAAAACAA TGAAGAGGAA TTCACCTCAA AAGATAAAAA AAAGAAAGGA 
CAGAAGGGCA AAAAACAGAG TTTTGATGAT AATGATAGCG AAGAATTGGA AGATAAAGAT 
TCAAAATCAA AAAAGACTGC AAAACCGAAA GTGGAAATGT ACTCTGGGAG TGATGATGAT 
GATGATTTTA ACAAACTTCC TAAAAAAGCT AAAGGGAAAG CTCAAAAATC AAATAAGAAG 
TGGGATGGGT CAGAGGAGGA TGAGGATAAC AGTAAAAAAA TTAAAGAGCG TTCAAGAATA 
AATTCTTCTG GTGAAAGTGG TGATGAATCA GATGAATTTT TGCAATCTAG AAAAGGACAG 
AAAAAAAATC AGAAAAACAA GCCAGGTCCT AACATAGAAA GTGGGAATGA AGATGATGAC 
GCCTCCTTCA AAATTAAGAC AGTGGCCCAA AAGAAGGCAG AAAAGAAGGA GCGCGAGAGA 
AAAAAGCGAG ATGAAGAAAA AGCGAAACTG CGGAAGCTGA AAGAAAAAGA AGAGTTAGAA 
ACAGGTAAAA AGGATCAGAG TAAACAAAAG GAATCTCAAA GGAAATTTGA AGAAGAAACT 
GTAAAATCCA AAGTGACTGT TGATACTGGA GTAATTCCTG CCTCTGAAGA GAAAGCAGAG 
ACTCCCACAG CTGCAGAAGA TGACAATGAA GGAGACAAAA AGAAGAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCCACT 
GTTAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAGG AAGAAGAAAG ACAGAAGAGA 
GAAGAGGAAG AACGTATAAA ACGGCTTGAA GAATTAGAAG CCAAGCGTAA AGAAGAGGAA 
CGATTGGAAC AAGAAAAAAG AGAAAGGAAA AAGCAAAAAG AAAAAGAAAG AAAAGAACGC 
TTGAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCCGAA 
GCTACTCTTA AACTGCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAGAGGC CAATTTATGA AGATAAAAAG AGGAAAAAAA TACCACAGCA GCTAGAAAGT 
AAAGAAGTGT CTGAATCAAT GGAATTATGT GCTGCTGTAG AAGTTATGGA ACAAGGAGTA 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AAGAAGAAGA TACTGAGGAT 
GCTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAGG AGACAGAAAA AGTAGAAGGA 
AACAAAGTTC ATATAGAAGT AAAAGAAAAC CCTGAAGAGG AGGAGGAGGA GGAAGAAGAG 
GAAGAAGAAG ATGAAGAAAG TGAAGAAGAG GAGGAAGAGG AGGGAGAAAG TGAAGGCAGT 
GAAGGTGATG AGGAAGATGA AAAGGTGTCA GATGAGAAGG ATTCAGGGAA GACATTAGAT 
AAAAAGCCAA GTAAAGAAAT GAGCTCAGAT TCTGAATATG ACTCTGATGA TGATCGGACT 
AAAGAAGAAA GGGCTTATGA CAAAGCAAAA CGGAGGATTG AGAAACGGCG ACTTGAACAT 
AGTAAAAATG TAAACACCGA AAAGCTAAGA GCCCCTATTA TCTGCGTACT TGGGCATGTG 
GACACAGGGA AGACAAAAAT TCTAGATAAG CTCCGTCACA CACATGTACA AGATGGTGAA 
GCAGGTGGTA TCACACAACA AATTGGGGCC ACCAATGTTC CTCTTGAAGC TATTAATGAA 
CAGACTAAGA TGATTAAAAA TTTTGATAGA GAGAATGTAC GGATTCCAGG AATGCTAATT 
ATTGATACTC CTGGGCATGA ATCTTTCAGT AATCTGAGAA ATAGAGGAAG CTCTCTTTGT 
GACATTGCCA TTTTAGTTGT TGATATTATG CATGGTTTGG AGCCCCAGAC AATTGAGTCT 
ATCAACCTTC TCAAATCTAA AAAATGTCCC TTCATTGTTG CACTCAATAA GATTGATAGG 
TTATATGATT GGAAAAAGAG TCCTGACTCT GATGTGGCTG CTACTTTAAA GAAGCAGAAA 
AAGAATACAA AAGATGAATT TGAGGAGCGA GCAAAGGCTA TTATTGTAGA ATTTGCACAG 
CAGGGTTTGA ATGCTGCTTT GTTTTATGAG AATAAAGATC CCCGCACTTT TGTGTCTTTG 
GTACCTACCT CTGCACATAC TGGTGATGGC ATGGGAAGTC TGATCTACCT TCTTGTAGAG 
TTAACTCAGA CCATGTTGAG CAAGAGACTT GCACACTGTG AAGAGCTGAG AGCACAGGTG 
ATGGAGGTTA AAGCTCTCCC GGGGATGGGC ACCACTATAG ATGTCATCTT GATCAATGGG 
CGTTTGAAGG AAGGAGATAC AATCATTGTT CCTGGAGTAG AAGGGCCCAT TGTAACTCAG 
ATTCGAGGCC TCCTGTTACC TCCTCCTATG AAGGAATTAC GAGTGAAGAA CCAGTATGAA 
AAGCATAAAG AAGTAGAAGC AGCTCAGGGG GTAAAGATTC TTGGAAAAGA CCTGGAGAAA 
ACATTGGCTG GTTTACCCCT CCTTGTGGCT TATAAAGAAG ATGAAATCCC TGTTCTTAAA 
GATGAATTGA TCCATGAGTT AAAGCAGACA CTAAATGCTA TCAAATTAGA AGAAAAAGGA 
GTCTATGTCC AGGCATCTAC ACTGGGTTCT TTGGAAGCTC TACTGGAATT TCTGAAAACA 
TCAGAAGTGC CCTATGCAGG AATTAACATT GGCCCAGTGC ATAAAAAAGA TGTTATGAAG 
GCTTCAGTGA TGTTGGAACA TGACCCTCAG TATGCAGTAA TTTTGGCCTT CGATGTGAGA 
ATTGAACGAG ATGCACAAGA AATGGCTGAT AGTTTAGGAG TTAGAATTTT TAGTGCAGAA 
ATTATTTATC ATTTATTTGA TGCCTTTACA AAATATAGAC AAGACTACAA GAAACAGAAA 
CAAGAAGAAT TTAAGCACAT AGCAGTATTT CCCTGCAAGA TAAAAATCCT CCCTCAGTAC 
ATTTTTAATT CTCGAGATCC GATAGTGATG GGGGTGACGG TGGAAGCAGG TCAGGTGAAA 
CAGGGGACAC CCATGTGTGT CCCAAGCAAA AATTTTGTTG ACATCGGAAT AGTAACAAGT 
ATTGAAATAA ACCATAAACA AGTGGATGTT GCAAAAAAAG GACAAGAAGT TTGTGTAAAA 
ATAGAACCTA TCCCTGGTGA GTCACCCAAA ATGTTTGGAA GACATTTTGA AGCTACAGAT 
ATTCTTGTTA GTAAGATCAG CCGGCAGTCC ATTGATGCAC TCAAAGACTG GTTCAGAGAT 
GAAATGCAGA AGAGTGACTG GCAGCTTATT GTGGAGCTGA AGAAAGTATT TGAAATCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( NM_015904.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056988.3 Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Price & Availability↑

CloneID	OHu30523
Clone ID Related Accession (Same CDS sequence)	NM_015904.3 , NM_015904.4
Accession Version	NM_015904.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX485839.1, AB018284.1, BC032639.1 and AC018690.5. On Nov 22, 2018 this sequence version replaced NM_015904.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018284.1, AJ006776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289371.11/ ENSP00000289371.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAAG AACAGGAGCC TCAAAAGTCA 
AAAGGGAAAA AGAAAAAAGA GAAAAAAAAG CAGGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTT GGAAGCTCAA GGCATCAAAG CTGACAGAGA AACTGTTGCA 
GTGAAGCCAA CAGAAAACAA TGAAGAGGAA TTCACCTCAA AAGATAAAAA AAAGAAAGGA 
CAGAAGGGCA AAAAACAGAG TTTTGATGAT AATGATAGCG AAGAATTGGA AGATAAAGAT 
TCAAAATCAA AAAAGACTGC AAAACCGAAA GTGGAAATGT ACTCTGGGAG TGATGATGAT 
GATGATTTTA ACAAACTTCC TAAAAAAGCT AAAGGGAAAG CTCAAAAATC AAATAAGAAG 
TGGGATGGGT CAGAGGAGGA TGAGGATAAC AGTAAAAAAA TTAAAGAGCG TTCAAGAATA 
AATTCTTCTG GTGAAAGTGG TGATGAATCA GATGAATTTT TGCAATCTAG AAAAGGACAG 
AAAAAAAATC AGAAAAACAA GCCAGGTCCT AACATAGAAA GTGGGAATGA AGATGATGAC 
GCCTCCTTCA AAATTAAGAC AGTGGCCCAA AAGAAGGCAG AAAAGAAGGA GCGCGAGAGA 
AAAAAGCGAG ATGAAGAAAA AGCGAAACTG CGGAAGCTGA AAGAAAAAGA AGAGTTAGAA 
ACAGGTAAAA AGGATCAGAG TAAACAAAAG GAATCTCAAA GGAAATTTGA AGAAGAAACT 
GTAAAATCCA AAGTGACTGT TGATACTGGA GTAATTCCTG CCTCTGAAGA GAAAGCAGAG 
ACTCCCACAG CTGCAGAAGA TGACAATGAA GGAGACAAAA AGAAGAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCCACT 
GTTAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAGG AAGAAGAAAG ACAGAAGAGA 
GAAGAGGAAG AACGTATAAA ACGGCTTGAA GAATTAGAAG CCAAGCGTAA AGAAGAGGAA 
CGATTGGAAC AAGAAAAAAG AGAAAGGAAA AAGCAAAAAG AAAAAGAAAG AAAAGAACGC 
TTGAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCCGAA 
GCTACTCTTA AACTGCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAGAGGC CAATTTATGA AGATAAAAAG AGGAAAAAAA TACCACAGCA GCTAGAAAGT 
AAAGAAGTGT CTGAATCAAT GGAATTATGT GCTGCTGTAG AAGTTATGGA ACAAGGAGTA 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AAGAAGAAGA TACTGAGGAT 
GCTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAGG AGACAGAAAA AGTAGAAGGA 
AACAAAGTTC ATATAGAAGT AAAAGAAAAC CCTGAAGAGG AGGAGGAGGA GGAAGAAGAG 
GAAGAAGAAG ATGAAGAAAG TGAAGAAGAG GAGGAAGAGG AGGGAGAAAG TGAAGGCAGT 
GAAGGTGATG AGGAAGATGA AAAGGTGTCA GATGAGAAGG ATTCAGGGAA GACATTAGAT 
AAAAAGCCAA GTAAAGAAAT GAGCTCAGAT TCTGAATATG ACTCTGATGA TGATCGGACT 
AAAGAAGAAA GGGCTTATGA CAAAGCAAAA CGGAGGATTG AGAAACGGCG ACTTGAACAT 
AGTAAAAATG TAAACACCGA AAAGCTAAGA GCCCCTATTA TCTGCGTACT TGGGCATGTG 
GACACAGGGA AGACAAAAAT TCTAGATAAG CTCCGTCACA CACATGTACA AGATGGTGAA 
GCAGGTGGTA TCACACAACA AATTGGGGCC ACCAATGTTC CTCTTGAAGC TATTAATGAA 
CAGACTAAGA TGATTAAAAA TTTTGATAGA GAGAATGTAC GGATTCCAGG AATGCTAATT 
ATTGATACTC CTGGGCATGA ATCTTTCAGT AATCTGAGAA ATAGAGGAAG CTCTCTTTGT 
GACATTGCCA TTTTAGTTGT TGATATTATG CATGGTTTGG AGCCCCAGAC AATTGAGTCT 
ATCAACCTTC TCAAATCTAA AAAATGTCCC TTCATTGTTG CACTCAATAA GATTGATAGG 
TTATATGATT GGAAAAAGAG TCCTGACTCT GATGTGGCTG CTACTTTAAA GAAGCAGAAA 
AAGAATACAA AAGATGAATT TGAGGAGCGA GCAAAGGCTA TTATTGTAGA ATTTGCACAG 
CAGGGTTTGA ATGCTGCTTT GTTTTATGAG AATAAAGATC CCCGCACTTT TGTGTCTTTG 
GTACCTACCT CTGCACATAC TGGTGATGGC ATGGGAAGTC TGATCTACCT TCTTGTAGAG 
TTAACTCAGA CCATGTTGAG CAAGAGACTT GCACACTGTG AAGAGCTGAG AGCACAGGTG 
ATGGAGGTTA AAGCTCTCCC GGGGATGGGC ACCACTATAG ATGTCATCTT GATCAATGGG 
CGTTTGAAGG AAGGAGATAC AATCATTGTT CCTGGAGTAG AAGGGCCCAT TGTAACTCAG 
ATTCGAGGCC TCCTGTTACC TCCTCCTATG AAGGAATTAC GAGTGAAGAA CCAGTATGAA 
AAGCATAAAG AAGTAGAAGC AGCTCAGGGG GTAAAGATTC TTGGAAAAGA CCTGGAGAAA 
ACATTGGCTG GTTTACCCCT CCTTGTGGCT TATAAAGAAG ATGAAATCCC TGTTCTTAAA 
GATGAATTGA TCCATGAGTT AAAGCAGACA CTAAATGCTA TCAAATTAGA AGAAAAAGGA 
GTCTATGTCC AGGCATCTAC ACTGGGTTCT TTGGAAGCTC TACTGGAATT TCTGAAAACA 
TCAGAAGTGC CCTATGCAGG AATTAACATT GGCCCAGTGC ATAAAAAAGA TGTTATGAAG 
GCTTCAGTGA TGTTGGAACA TGACCCTCAG TATGCAGTAA TTTTGGCCTT CGATGTGAGA 
ATTGAACGAG ATGCACAAGA AATGGCTGAT AGTTTAGGAG TTAGAATTTT TAGTGCAGAA 
ATTATTTATC ATTTATTTGA TGCCTTTACA AAATATAGAC AAGACTACAA GAAACAGAAA 
CAAGAAGAAT TTAAGCACAT AGCAGTATTT CCCTGCAAGA TAAAAATCCT CCCTCAGTAC 
ATTTTTAATT CTCGAGATCC GATAGTGATG GGGGTGACGG TGGAAGCAGG TCAGGTGAAA 
CAGGGGACAC CCATGTGTGT CCCAAGCAAA AATTTTGTTG ACATCGGAAT AGTAACAAGT 
ATTGAAATAA ACCATAAACA AGTGGATGTT GCAAAAAAAG GACAAGAAGT TTGTGTAAAA 
ATAGAACCTA TCCCTGGTGA GTCACCCAAA ATGTTTGGAA GACATTTTGA AGCTACAGAT 
ATTCTTGTTA GTAAGATCAG CCGGCAGTCC ATTGATGCAC TCAAAGACTG GTTCAGAGAT 
GAAATGCAGA AGAGTGACTG GCAGCTTATT GTGGAGCTGA AGAAAGTATT TGAAATCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056988.3
CDS	167..3829
Translation	MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	NM_015904.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015904.4

ORF Insert Sequence:

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAAAG AACAGGAGCC TCAAAAGTCA 
AAAGGGAAAA AGAAAAAAGA GAAAAAAAAG CAGGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTT GGAAGCTCAA GGCATCAAAG CTGACAGAGA AACTGTTGCA 
GTGAAGCCAA CAGAAAACAA TGAAGAGGAA TTCACCTCAA AAGATAAAAA AAAGAAAGGA 
CAGAAGGGCA AAAAACAGAG TTTTGATGAT AATGATAGCG AAGAATTGGA AGATAAAGAT 
TCAAAATCAA AAAAGACTGC AAAACCGAAA GTGGAAATGT ACTCTGGGAG TGATGATGAT 
GATGATTTTA ACAAACTTCC TAAAAAAGCT AAAGGGAAAG CTCAAAAATC AAATAAGAAG 
TGGGATGGGT CAGAGGAGGA TGAGGATAAC AGTAAAAAAA TTAAAGAGCG TTCAAGAATA 
AATTCTTCTG GTGAAAGTGG TGATGAATCA GATGAATTTT TGCAATCTAG AAAAGGACAG 
AAAAAAAATC AGAAAAACAA GCCAGGTCCT AACATAGAAA GTGGGAATGA AGATGATGAC 
GCCTCCTTCA AAATTAAGAC AGTGGCCCAA AAGAAGGCAG AAAAGAAGGA GCGCGAGAGA 
AAAAAGCGAG ATGAAGAAAA AGCGAAACTG CGGAAGCTGA AAGAAAAAGA AGAGTTAGAA 
ACAGGTAAAA AGGATCAGAG TAAACAAAAG GAATCTCAAA GGAAATTTGA AGAAGAAACT 
GTAAAATCCA AAGTGACTGT TGATACTGGA GTAATTCCTG CCTCTGAAGA GAAAGCAGAG 
ACTCCCACAG CTGCAGAAGA TGACAATGAA GGAGACAAAA AGAAGAAAGA TAAGAAGAAA 
AAGAAAGGAG AAAAGGAAGA AAAAGAGAAA GAGAAGAAAA AAGGACCTAG CAAAGCCACT 
GTTAAAGCTA TGCAAGAAGC TCTGGCTAAG CTTAAAGAGG AAGAAGAAAG ACAGAAGAGA 
GAAGAGGAAG AACGTATAAA ACGGCTTGAA GAATTAGAAG CCAAGCGTAA AGAAGAGGAA 
CGATTGGAAC AAGAAAAAAG AGAAAGGAAA AAGCAAAAAG AAAAAGAAAG AAAAGAACGC 
TTGAAAAAAG AAGGGAAACT TTTAACTAAA TCCCAGAGAG AAGCCAGAGC CAGAGCCGAA 
GCTACTCTTA AACTGCTACA AGCTCAGGGT GTTGAAGTGC CATCAAAAGA CTCTTTGCCA 
AAGAAGAGGC CAATTTATGA AGATAAAAAG AGGAAAAAAA TACCACAGCA GCTAGAAAGT 
AAAGAAGTGT CTGAATCAAT GGAATTATGT GCTGCTGTAG AAGTTATGGA ACAAGGAGTA 
CCAGAAAAGG AAGAGACACC ACCTCCTGTT GAACCAGAAG AAGAAGAAGA TACTGAGGAT 
GCTGGATTGG ATGATTGGGA AGCTATGGCC AGTGATGAGG AGACAGAAAA AGTAGAAGGA 
AACAAAGTTC ATATAGAAGT AAAAGAAAAC CCTGAAGAGG AGGAGGAGGA GGAAGAAGAG 
GAAGAAGAAG ATGAAGAAAG TGAAGAAGAG GAGGAAGAGG AGGGAGAAAG TGAAGGCAGT 
GAAGGTGATG AGGAAGATGA AAAGGTGTCA GATGAGAAGG ATTCAGGGAA GACATTAGAT 
AAAAAGCCAA GTAAAGAAAT GAGCTCAGAT TCTGAATATG ACTCTGATGA TGATCGGACT 
AAAGAAGAAA GGGCTTATGA CAAAGCAAAA CGGAGGATTG AGAAACGGCG ACTTGAACAT 
AGTAAAAATG TAAACACCGA AAAGCTAAGA GCCCCTATTA TCTGCGTACT TGGGCATGTG 
GACACAGGGA AGACAAAAAT TCTAGATAAG CTCCGTCACA CACATGTACA AGATGGTGAA 
GCAGGTGGTA TCACACAACA AATTGGGGCC ACCAATGTTC CTCTTGAAGC TATTAATGAA 
CAGACTAAGA TGATTAAAAA TTTTGATAGA GAGAATGTAC GGATTCCAGG AATGCTAATT 
ATTGATACTC CTGGGCATGA ATCTTTCAGT AATCTGAGAA ATAGAGGAAG CTCTCTTTGT 
GACATTGCCA TTTTAGTTGT TGATATTATG CATGGTTTGG AGCCCCAGAC AATTGAGTCT 
ATCAACCTTC TCAAATCTAA AAAATGTCCC TTCATTGTTG CACTCAATAA GATTGATAGG 
TTATATGATT GGAAAAAGAG TCCTGACTCT GATGTGGCTG CTACTTTAAA GAAGCAGAAA 
AAGAATACAA AAGATGAATT TGAGGAGCGA GCAAAGGCTA TTATTGTAGA ATTTGCACAG 
CAGGGTTTGA ATGCTGCTTT GTTTTATGAG AATAAAGATC CCCGCACTTT TGTGTCTTTG 
GTACCTACCT CTGCACATAC TGGTGATGGC ATGGGAAGTC TGATCTACCT TCTTGTAGAG 
TTAACTCAGA CCATGTTGAG CAAGAGACTT GCACACTGTG AAGAGCTGAG AGCACAGGTG 
ATGGAGGTTA AAGCTCTCCC GGGGATGGGC ACCACTATAG ATGTCATCTT GATCAATGGG 
CGTTTGAAGG AAGGAGATAC AATCATTGTT CCTGGAGTAG AAGGGCCCAT TGTAACTCAG 
ATTCGAGGCC TCCTGTTACC TCCTCCTATG AAGGAATTAC GAGTGAAGAA CCAGTATGAA 
AAGCATAAAG AAGTAGAAGC AGCTCAGGGG GTAAAGATTC TTGGAAAAGA CCTGGAGAAA 
ACATTGGCTG GTTTACCCCT CCTTGTGGCT TATAAAGAAG ATGAAATCCC TGTTCTTAAA 
GATGAATTGA TCCATGAGTT AAAGCAGACA CTAAATGCTA TCAAATTAGA AGAAAAAGGA 
GTCTATGTCC AGGCATCTAC ACTGGGTTCT TTGGAAGCTC TACTGGAATT TCTGAAAACA 
TCAGAAGTGC CCTATGCAGG AATTAACATT GGCCCAGTGC ATAAAAAAGA TGTTATGAAG 
GCTTCAGTGA TGTTGGAACA TGACCCTCAG TATGCAGTAA TTTTGGCCTT CGATGTGAGA 
ATTGAACGAG ATGCACAAGA AATGGCTGAT AGTTTAGGAG TTAGAATTTT TAGTGCAGAA 
ATTATTTATC ATTTATTTGA TGCCTTTACA AAATATAGAC AAGACTACAA GAAACAGAAA 
CAAGAAGAAT TTAAGCACAT AGCAGTATTT CCCTGCAAGA TAAAAATCCT CCCTCAGTAC 
ATTTTTAATT CTCGAGATCC GATAGTGATG GGGGTGACGG TGGAAGCAGG TCAGGTGAAA 
CAGGGGACAC CCATGTGTGT CCCAAGCAAA AATTTTGTTG ACATCGGAAT AGTAACAAGT 
ATTGAAATAA ACCATAAACA AGTGGATGTT GCAAAAAAAG GACAAGAAGT TTGTGTAAAA 
ATAGAACCTA TCCCTGGTGA GTCACCCAAA ATGTTTGGAA GACATTTTGA AGCTACAGAT 
ATTCTTGTTA GTAAGATCAG CCGGCAGTCC ATTGATGCAC TCAAAGACTG GTTCAGAGAT 
GAAATGCAGA AGAGTGACTG GCAGCTTATT GTGGAGCTGA AGAAAGTATT TGAAATCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
Cell reports22(1)17-26(2018 Jan)
Ho JJD,Balukoff NC,Cervantes G,Malcolm PD,Krieger JR,Lee S

eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
Oncotarget7(38)62327-62339(2016 09)
Wang ZG,Zheng H,Gao W,Han J,Cao JZ,Yang Y,Li S,Gao R,Liu H,Pan ZY,Fu SY,Gu FM,Xing H,Ni JS,Yan HL,Ren H,Zhou WP

eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling.
Nucleic acids research44(15)7441-56(2016 Sep)
Nag N,Lin KY,Edmonds KA,Yu J,Nadkarni D,Marintcheva B,Marintchev A

Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
PloS one11(12)e0168387(2016)
Jiang X,Jiang X,Feng Y,Xu R,Wang Q,Deng H

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EIF5B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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