EIF5B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF5B
Entrez Gene ID	532850
Full Name	eukaryotic translation initiation factor 5B
General protein information	Preferred Names eukaryotic translation initiation factor 5B Names LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B eukaryotic translation initiation factor 5B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003586607.4	XP_003586655.1	LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
XM_003582755.4	XP_003582803.2	LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
XM_003586607.5	XP_003586655.2	eukaryotic translation initiation factor 5B isoform X1
XM_024999310.1	XP_024855078.1	eukaryotic translation initiation factor 5B isoform X3
XM_024999311.1	XP_024855079.1	eukaryotic translation initiation factor 5B isoform X4
XM_024999309.1	XP_024855077.1	eukaryotic translation initiation factor 5B isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF5B	NP_056988.3
Rattus norvegicus (Norway rat)	Eif5b	NP_001103611.1
Bos taurus (cattle)	EIF5B	XP_003586655.1
Gallus gallus (chicken)	EIF5B	NP_001033585.1
Pan troglodytes (chimpanzee)	EIF5B	XP_001159759.1
Canis lupus familiaris (dog)	EIF5B	XP_005626079.1
	EIF5B	XP_001103720.2
Xenopus tropicalis (tropical clawed frog)	eif5b	XP_004911863.1
Mus musculus (house mouse)	Eif5b	NP_938045.2
Danio rerio (zebrafish)	eif5b	XP_001923573.4

Gene Ontology

The following EIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220729	XM_003586607.5 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220730	XM_024999309.1 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220731	XM_024999310.1 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220732	XM_024999311.1 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa23755	XM_003586607.4 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa23755	XM_003582755.4 Latest version!	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF5B ( XM_003586607.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586655.2 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

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CloneID	OBa220729
Clone ID Related Accession (Same CDS sequence)	XM_003586607.5
Accession Version	XM_003586607.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586607.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586655.2
CDS	279..3938
Translation	MGKKQKNKNEDSAKDDIDLDALAAEIEGAGAAREQEPQKAKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADREPAAVKPTENNEDEPISKQDKKKKGQKGKKQSFEDNDSEELEEKDSKSKKTAKPKVEMYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKPPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETEENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_003586607.5
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586607.5

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_024999309.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855077.1 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220730
Clone ID Related Accession (Same CDS sequence)	XM_024999309.1
Accession Version	XM_024999309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAAACACG 
GTGCATATAG AAGTAAAAGA AAACCCTGAA GAGGAGGAGG AGGAGGAAGA GGAGGAGGAG 
GAGGAAGAAG AGAGTGAAGA CGAAGAGGAG GAAGAAGGAG ACAGTGAAGG CAGTGAAGGT 
GAAGATGATG ATGAAAAGGT GTCAGATGAG AAGGACGCTG GGAAAACATT GGATAAAAAG 
CCAAGCAAAG ACGTGAGCTC AGAATCTGAG GATGACTCTG ACGACGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC AAAACGGAGG ATTGAGAAAC GGCGACTTGA ACACAGTAAA 
AATGTAAATA CAGAGAAGCT GAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGATACA 
GGGAAGACAA AAATTCTAGA TAAGCTCCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGTATCACAC AGCAAATTGG TGCCACCAAT GTCCCTCTTG AAGCTATTAA TGAACAAACT 
AAGATGATTA AAAATTTTGA TCGAGAGAAC GTACGGATTC CAGGAATGCT GATTATTGAC 
ACTCCGGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGACATT 
GCCATTTTAG TTGTTGATAT TATGCATGGT TTGGAGCCCC AGACAATTGA ATCTATCAAC 
CTTTTGAAAT CTAAAAAATG TCCCTTCATT GTTGCCCTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGACGTG GCCGCTACTC TGAAGAAGCA GAAGAAGAAC 
ACCAGAGATG AATTTGAGGA GCGGGCCAAG GCGATCATTG TGGAATTTGC ACAGCAGGGG 
CTGAATGCAG CTTTGTTCTA TGAGAATAAG GATCCCCGCA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCAC ACACTGGCGA TGGCATGGGA AGTCTGATCT GCCTTCTTGT TGAGTTGACT 
CAGACCATGT TGAGTAAGAG GCTTGCCCAG TGTGAGGAGC TGAGAGCCCA GGTGATGGAG 
GTTAAGGCTC TCCCGGGAAT GGGCACGACT ATTGATGTAA TACTGATCAA CGGGCGTCTG 
AAGGAAGGAG ACACGATTAT TGTTCCCGGA GTAGAAGGGC CTATCGTCAC GCAGATCCGA 
GGCCTCCTGT TACCTCCCCC TATGAAGGAG TTAAGAGTGA AGAACCAATA TGAGAAGCAT 
AAAGAGGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AGGACCTGGA AAAAACATTG 
GCTGGTTTAC CCCTCCTCGT GGCTTATAAA GAAGATGAAG TCCCAGTTCT TAAAGATGAG 
CTGATCCACG AGTTAAAGCA GACACTGAAC GCTATCAAAT TAGAAGAGAA AGGCGTCTAC 
GTCCAGGCCT CGACCCTGGG ATCTCTGGAA GCTCTCCTTG AATTTCTGAA GACATCAGAA 
GTGCCCTACG CAGGGATCAA CATTGGGCCC GTCCACAAAA AAGACGTCAT GAAGGCTTCG 
GTGATGCTGG AACACGACCC TCAGTATGCA GTGATTTTGG CCTTCGATGT GAGAATTGAA 
CGGGATGCCC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAC AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ACATAGCAGT ATTCCCCTGC AAGATAAAGA TCCTCCCTCA GTTTATCTTC 
AATTCTCGGG ATCCGATAGT GATGGGCGTG ACTGTGGAAG CAGGGCAGGT GAAGCAGGGG 
ACACCCATGT GCGTGCCCAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
GTAAACCATA AACAAGTGGA TGTTGCGAAA AAAGGCCAAG AAGTCTGTGT CAAAATAGAA 
CCCATCCCAG GTGAATCTCC CAAAATGTAT GGACGCCATT TTGAAGCTAC AGACATTCTC 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCTCTCAAAG ACTGGTTCAG AGACGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TGTTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855077.1
CDS	279..3935
Translation	MGKKQKNKNEDSAKDDIDLDALAAEIEGAGAAREQEPQKAKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADREPAAVKPTENNEDEPISKQDKKKKGQKGKKQSFEDNDSEELEEKDSKSKKTAKPKVEMYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKPPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_024999309.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999309.1

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAAACACG 
GTGCATATAG AAGTAAAAGA AAACCCTGAA GAGGAGGAGG AGGAGGAAGA GGAGGAGGAG 
GAGGAAGAAG AGAGTGAAGA CGAAGAGGAG GAAGAAGGAG ACAGTGAAGG CAGTGAAGGT 
GAAGATGATG ATGAAAAGGT GTCAGATGAG AAGGACGCTG GGAAAACATT GGATAAAAAG 
CCAAGCAAAG ACGTGAGCTC AGAATCTGAG GATGACTCTG ACGACGATCG AACTAAAGAA 
GAACGGGCTT ATGACAAAGC AAAACGGAGG ATTGAGAAAC GGCGACTTGA ACACAGTAAA 
AATGTAAATA CAGAGAAGCT GAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGATACA 
GGGAAGACAA AAATTCTAGA TAAGCTCCGT CACACACATG TACAAGATGG TGAAGCAGGT 
GGTATCACAC AGCAAATTGG TGCCACCAAT GTCCCTCTTG AAGCTATTAA TGAACAAACT 
AAGATGATTA AAAATTTTGA TCGAGAGAAC GTACGGATTC CAGGAATGCT GATTATTGAC 
ACTCCGGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGACATT 
GCCATTTTAG TTGTTGATAT TATGCATGGT TTGGAGCCCC AGACAATTGA ATCTATCAAC 
CTTTTGAAAT CTAAAAAATG TCCCTTCATT GTTGCCCTCA ATAAGATTGA TAGGTTGTAT 
GATTGGAAAA AGAGCCCTGA CTCTGACGTG GCCGCTACTC TGAAGAAGCA GAAGAAGAAC 
ACCAGAGATG AATTTGAGGA GCGGGCCAAG GCGATCATTG TGGAATTTGC ACAGCAGGGG 
CTGAATGCAG CTTTGTTCTA TGAGAATAAG GATCCCCGCA CTTTTGTGTC CTTGGTACCT 
ACCTCTGCAC ACACTGGCGA TGGCATGGGA AGTCTGATCT GCCTTCTTGT TGAGTTGACT 
CAGACCATGT TGAGTAAGAG GCTTGCCCAG TGTGAGGAGC TGAGAGCCCA GGTGATGGAG 
GTTAAGGCTC TCCCGGGAAT GGGCACGACT ATTGATGTAA TACTGATCAA CGGGCGTCTG 
AAGGAAGGAG ACACGATTAT TGTTCCCGGA GTAGAAGGGC CTATCGTCAC GCAGATCCGA 
GGCCTCCTGT TACCTCCCCC TATGAAGGAG TTAAGAGTGA AGAACCAATA TGAGAAGCAT 
AAAGAGGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AGGACCTGGA AAAAACATTG 
GCTGGTTTAC CCCTCCTCGT GGCTTATAAA GAAGATGAAG TCCCAGTTCT TAAAGATGAG 
CTGATCCACG AGTTAAAGCA GACACTGAAC GCTATCAAAT TAGAAGAGAA AGGCGTCTAC 
GTCCAGGCCT CGACCCTGGG ATCTCTGGAA GCTCTCCTTG AATTTCTGAA GACATCAGAA 
GTGCCCTACG CAGGGATCAA CATTGGGCCC GTCCACAAAA AAGACGTCAT GAAGGCTTCG 
GTGATGCTGG AACACGACCC TCAGTATGCA GTGATTTTGG CCTTCGATGT GAGAATTGAA 
CGGGATGCCC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT 
TATCATTTAT TTGATGCCTT TACGAAATAC AGACAAGACT ACAAGAAACA GAAACAAGAA 
GAATTTAAGC ACATAGCAGT ATTCCCCTGC AAGATAAAGA TCCTCCCTCA GTTTATCTTC 
AATTCTCGGG ATCCGATAGT GATGGGCGTG ACTGTGGAAG CAGGGCAGGT GAAGCAGGGG 
ACACCCATGT GCGTGCCCAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA 
GTAAACCATA AACAAGTGGA TGTTGCGAAA AAAGGCCAAG AAGTCTGTGT CAAAATAGAA 
CCCATCCCAG GTGAATCTCC CAAAATGTAT GGACGCCATT TTGAAGCTAC AGACATTCTC 
GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCTCTCAAAG ACTGGTTCAG AGACGAGATG 
CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TGTTTGAAAT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_024999310.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855078.1 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

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CloneID	OBa220731
Clone ID Related Accession (Same CDS sequence)	XM_024999310.1
Accession Version	XM_024999310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT GCCTTTTATT TCTTGCCAAG GATGACATTG ATCTTGATGC CTTGGCTGCA 
GAAATAGAAG GAGCTGGTGC TGCCAGGGAG CAAGAACCTC AAAAGGCAAA GGGGAAAAAG 
AAAAAGGAGA AAAAAAGACA AGACTTTGAT GAAGATGATA TCCTGAAAGA ACTGGAAGAA 
TTGTCTTGGG AAGCTCAAGG CATCAAAGCT GACAGAGAGC CTGCTGCGGT CAAGCCAACA 
GAAAATAATG AAGATGAACC CATCTCAAAA CAAGATAAGA AAAAGAAAGG ACAGAAAGGC 
AAAAAACAGA GTTTTGAAGA TAATGATAGT GAAGAATTGG AAGAAAAAGA TTCAAAGTCA 
AAAAAGACTG CAAAACCCAA AGTGGAAATG TACTCTGGGA GTGATGATGA TGATGATTTT 
AATAAACTAT CCAAAAAAGC AAAGGGGAAA GCTCAGAAAT CAAATAAGAA GCGTGATGCA 
TCAGAAGAGG ATGAGGATAA CAGTAAAAGA ATTAAAGAGC GTATGAGAGC ACATTCTTCT 
GGTGAAAGTG GTGATGAATC AGATGAATTT TTGCAATCCA GGAAAGGACA GAAAAAAAAT 
CAGAAAAACA AGCCAGGTCC TACTGTCGAG AGTGGAAATG AAGATGATGA CTCGTCCTTC 
AAAATTAAGA CAGTGGCTCA AAAGAAAGCA GAGAAGAAAG AGCGTGAGAG AAAAAAACGG 
GATGAAGAAA AGGCAAAACT TCGGAAGCTG AAAGAAAAAG AAGAGCTGGA GTCTGGTAAA 
AAGGATCTGG GTAAACCAAA AGAATCTCAG CGGAAACCTG AGGAGGAAGC TCTGAAATCC 
AAAGTGACAC TTGACTCTGG GGCAGCTCCT GCCTCTGAAG AGAAAGGAGA GGGTCCCATA 
GGCGCAGAAG ATGACAATGA AGGAGACAAA AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA 
GAAAAGGAAG AAAAGGAGAA AGAAAAGAAG AAAGGACCTA GCAAAGCCAC AGTTAAGGCT 
ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA GAGGAGGAAA GACAGAAGAG AGAGGAGGAA 
GAACGGATAA AACGGCTTGA AGAGTTAGAA GCCAAGCGTA AAGAAGAGGA AAGATTGGAA 
CAAGAAAAAA GAGAAAGGAA AAAGCAAAAA GAAAAGGAAA GGAAAGAACG CTTGAAAAAA 
GAGGGGAAAC TTTTAACTAA ATCCCAGAGA GAAGCCAGAG CCAGAGCCGA AGCTACCCTT 
AAACTTCTAC AAGCTCAGGG TGTTGAAGTG CCATCAAAAG ACTCTTTGCC AAAGAAGAGG 
CCAATTTATG AAGATAAAAA GAAGAAAAAA CCACCGCAGC AGATGGAAAA TAAAGTTTCT 
GAACCAGTGG AATTAAGTGC TGCTGCAGAA GTTGTGGAGC AAGGACTACC CGAAAAAGAA 
GAGACACCGC CTCCTGTTGA ACCAGAAGAG GAAGAAGAAA CCGAGGATGC TGGGTTGGAT 
GACTGGGAAG CTATGGCTAG CGATGAGGAG AGAGAGACAG AAGAAAACAC GGTGCATATA 
GAAGTAAAAG AAAACCCTGA AGAGGAGGAG GAGGAGGAAG AGGAGGAGGA GGAGGAAGAA 
GAGAGTGAAG ACGAAGAGGA GGAAGAAGGA GACAGTGAAG GCAGTGAAGG TGAAGATGAT 
GATGAAAAGG TGTCAGATGA GAAGGACGCT GGGAAAACAT TGGATAAAAA GCCAAGCAAA 
GACGTGAGCT CAGAATCTGA GGATGACTCT GACGACGATC GAACTAAAGA AGAACGGGCT 
TATGACAAAG CAAAACGGAG GATTGAGAAA CGGCGACTTG AACACAGTAA AAATGTAAAT 
ACAGAGAAGC TGAGAGCTCC TGTTATTTGT GTACTTGGAC ATGTGGATAC AGGGAAGACA 
AAAATTCTAG ATAAGCTCCG TCACACACAT GTACAAGATG GTGAAGCAGG TGGTATCACA 
CAGCAAATTG GTGCCACCAA TGTCCCTCTT GAAGCTATTA ATGAACAAAC TAAGATGATT 
AAAAATTTTG ATCGAGAGAA CGTACGGATT CCAGGAATGC TGATTATTGA CACTCCGGGG 
CATGAGTCTT TCAGTAATCT GAGAAACAGA GGAAGCTCTC TTTGTGACAT TGCCATTTTA 
GTTGTTGATA TTATGCATGG TTTGGAGCCC CAGACAATTG AATCTATCAA CCTTTTGAAA 
TCTAAAAAAT GTCCCTTCAT TGTTGCCCTC AATAAGATTG ATAGGTTGTA TGATTGGAAA 
AAGAGCCCTG ACTCTGACGT GGCCGCTACT CTGAAGAAGC AGAAGAAGAA CACCAGAGAT 
GAATTTGAGG AGCGGGCCAA GGCGATCATT GTGGAATTTG CACAGCAGGG GCTGAATGCA 
GCTTTGTTCT ATGAGAATAA GGATCCCCGC ACTTTTGTGT CCTTGGTACC TACCTCTGCA 
CACACTGGCG ATGGCATGGG AAGTCTGATC TGCCTTCTTG TTGAGTTGAC TCAGACCATG 
TTGAGTAAGA GGCTTGCCCA GTGTGAGGAG CTGAGAGCCC AGGTGATGGA GGTTAAGGCT 
CTCCCGGGAA TGGGCACGAC TATTGATGTA ATACTGATCA ACGGGCGTCT GAAGGAAGGA 
GACACGATTA TTGTTCCCGG AGTAGAAGGG CCTATCGTCA CGCAGATCCG AGGCCTCCTG 
TTACCTCCCC CTATGAAGGA GTTAAGAGTG AAGAACCAAT ATGAGAAGCA TAAAGAGGTA 
GAAGCAGCTC AGGGGGTAAA GATTCTTGGA AAGGACCTGG AAAAAACATT GGCTGGTTTA 
CCCCTCCTCG TGGCTTATAA AGAAGATGAA GTCCCAGTTC TTAAAGATGA GCTGATCCAC 
GAGTTAAAGC AGACACTGAA CGCTATCAAA TTAGAAGAGA AAGGCGTCTA CGTCCAGGCC 
TCGACCCTGG GATCTCTGGA AGCTCTCCTT GAATTTCTGA AGACATCAGA AGTGCCCTAC 
GCAGGGATCA ACATTGGGCC CGTCCACAAA AAAGACGTCA TGAAGGCTTC GGTGATGCTG 
GAACACGACC CTCAGTATGC AGTGATTTTG GCCTTCGATG TGAGAATTGA ACGGGATGCC 
CAAGAAATGG CTGATAGTTT AGGAGTTAGA ATTTTTAGTG CAGAAATTAT TTATCATTTA 
TTTGATGCCT TTACGAAATA CAGACAAGAC TACAAGAAAC AGAAACAAGA AGAATTTAAG 
CACATAGCAG TATTCCCCTG CAAGATAAAG ATCCTCCCTC AGTTTATCTT CAATTCTCGG 
GATCCGATAG TGATGGGCGT GACTGTGGAA GCAGGGCAGG TGAAGCAGGG GACACCCATG 
TGCGTGCCCA GCAAAAATTT TGTTGACATT GGAATAGTAA CAAGTATTGA AGTAAACCAT 
AAACAAGTGG ATGTTGCGAA AAAAGGCCAA GAAGTCTGTG TCAAAATAGA ACCCATCCCA 
GGTGAATCTC CCAAAATGTA TGGACGCCAT TTTGAAGCTA CAGACATTCT CGTCAGTAAG 
ATCAGCCGGC AGTCCATCGA TGCTCTCAAA GACTGGTTCA GAGACGAGAT GCAGAAGAGT 
GACTGGCAGC TTATTGTGGA GCTGAAGAAA GTGTTTGAAA TCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855078.1
CDS	19..3666
Translation	MENCLLFLAKDDIDLDALAAEIEGAGAAREQEPQKAKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADREPAAVKPTENNEDEPISKQDKKKKGQKGKKQSFEDNDSEELEEKDSKSKKTAKPKVEMYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKPPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETEENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_024999310.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999310.1

ORF Insert Sequence:

ATGGAAAATT GCCTTTTATT TCTTGCCAAG GATGACATTG ATCTTGATGC CTTGGCTGCA 
GAAATAGAAG GAGCTGGTGC TGCCAGGGAG CAAGAACCTC AAAAGGCAAA GGGGAAAAAG 
AAAAAGGAGA AAAAAAGACA AGACTTTGAT GAAGATGATA TCCTGAAAGA ACTGGAAGAA 
TTGTCTTGGG AAGCTCAAGG CATCAAAGCT GACAGAGAGC CTGCTGCGGT CAAGCCAACA 
GAAAATAATG AAGATGAACC CATCTCAAAA CAAGATAAGA AAAAGAAAGG ACAGAAAGGC 
AAAAAACAGA GTTTTGAAGA TAATGATAGT GAAGAATTGG AAGAAAAAGA TTCAAAGTCA 
AAAAAGACTG CAAAACCCAA AGTGGAAATG TACTCTGGGA GTGATGATGA TGATGATTTT 
AATAAACTAT CCAAAAAAGC AAAGGGGAAA GCTCAGAAAT CAAATAAGAA GCGTGATGCA 
TCAGAAGAGG ATGAGGATAA CAGTAAAAGA ATTAAAGAGC GTATGAGAGC ACATTCTTCT 
GGTGAAAGTG GTGATGAATC AGATGAATTT TTGCAATCCA GGAAAGGACA GAAAAAAAAT 
CAGAAAAACA AGCCAGGTCC TACTGTCGAG AGTGGAAATG AAGATGATGA CTCGTCCTTC 
AAAATTAAGA CAGTGGCTCA AAAGAAAGCA GAGAAGAAAG AGCGTGAGAG AAAAAAACGG 
GATGAAGAAA AGGCAAAACT TCGGAAGCTG AAAGAAAAAG AAGAGCTGGA GTCTGGTAAA 
AAGGATCTGG GTAAACCAAA AGAATCTCAG CGGAAACCTG AGGAGGAAGC TCTGAAATCC 
AAAGTGACAC TTGACTCTGG GGCAGCTCCT GCCTCTGAAG AGAAAGGAGA GGGTCCCATA 
GGCGCAGAAG ATGACAATGA AGGAGACAAA AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA 
GAAAAGGAAG AAAAGGAGAA AGAAAAGAAG AAAGGACCTA GCAAAGCCAC AGTTAAGGCT 
ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA GAGGAGGAAA GACAGAAGAG AGAGGAGGAA 
GAACGGATAA AACGGCTTGA AGAGTTAGAA GCCAAGCGTA AAGAAGAGGA AAGATTGGAA 
CAAGAAAAAA GAGAAAGGAA AAAGCAAAAA GAAAAGGAAA GGAAAGAACG CTTGAAAAAA 
GAGGGGAAAC TTTTAACTAA ATCCCAGAGA GAAGCCAGAG CCAGAGCCGA AGCTACCCTT 
AAACTTCTAC AAGCTCAGGG TGTTGAAGTG CCATCAAAAG ACTCTTTGCC AAAGAAGAGG 
CCAATTTATG AAGATAAAAA GAAGAAAAAA CCACCGCAGC AGATGGAAAA TAAAGTTTCT 
GAACCAGTGG AATTAAGTGC TGCTGCAGAA GTTGTGGAGC AAGGACTACC CGAAAAAGAA 
GAGACACCGC CTCCTGTTGA ACCAGAAGAG GAAGAAGAAA CCGAGGATGC TGGGTTGGAT 
GACTGGGAAG CTATGGCTAG CGATGAGGAG AGAGAGACAG AAGAAAACAC GGTGCATATA 
GAAGTAAAAG AAAACCCTGA AGAGGAGGAG GAGGAGGAAG AGGAGGAGGA GGAGGAAGAA 
GAGAGTGAAG ACGAAGAGGA GGAAGAAGGA GACAGTGAAG GCAGTGAAGG TGAAGATGAT 
GATGAAAAGG TGTCAGATGA GAAGGACGCT GGGAAAACAT TGGATAAAAA GCCAAGCAAA 
GACGTGAGCT CAGAATCTGA GGATGACTCT GACGACGATC GAACTAAAGA AGAACGGGCT 
TATGACAAAG CAAAACGGAG GATTGAGAAA CGGCGACTTG AACACAGTAA AAATGTAAAT 
ACAGAGAAGC TGAGAGCTCC TGTTATTTGT GTACTTGGAC ATGTGGATAC AGGGAAGACA 
AAAATTCTAG ATAAGCTCCG TCACACACAT GTACAAGATG GTGAAGCAGG TGGTATCACA 
CAGCAAATTG GTGCCACCAA TGTCCCTCTT GAAGCTATTA ATGAACAAAC TAAGATGATT 
AAAAATTTTG ATCGAGAGAA CGTACGGATT CCAGGAATGC TGATTATTGA CACTCCGGGG 
CATGAGTCTT TCAGTAATCT GAGAAACAGA GGAAGCTCTC TTTGTGACAT TGCCATTTTA 
GTTGTTGATA TTATGCATGG TTTGGAGCCC CAGACAATTG AATCTATCAA CCTTTTGAAA 
TCTAAAAAAT GTCCCTTCAT TGTTGCCCTC AATAAGATTG ATAGGTTGTA TGATTGGAAA 
AAGAGCCCTG ACTCTGACGT GGCCGCTACT CTGAAGAAGC AGAAGAAGAA CACCAGAGAT 
GAATTTGAGG AGCGGGCCAA GGCGATCATT GTGGAATTTG CACAGCAGGG GCTGAATGCA 
GCTTTGTTCT ATGAGAATAA GGATCCCCGC ACTTTTGTGT CCTTGGTACC TACCTCTGCA 
CACACTGGCG ATGGCATGGG AAGTCTGATC TGCCTTCTTG TTGAGTTGAC TCAGACCATG 
TTGAGTAAGA GGCTTGCCCA GTGTGAGGAG CTGAGAGCCC AGGTGATGGA GGTTAAGGCT 
CTCCCGGGAA TGGGCACGAC TATTGATGTA ATACTGATCA ACGGGCGTCT GAAGGAAGGA 
GACACGATTA TTGTTCCCGG AGTAGAAGGG CCTATCGTCA CGCAGATCCG AGGCCTCCTG 
TTACCTCCCC CTATGAAGGA GTTAAGAGTG AAGAACCAAT ATGAGAAGCA TAAAGAGGTA 
GAAGCAGCTC AGGGGGTAAA GATTCTTGGA AAGGACCTGG AAAAAACATT GGCTGGTTTA 
CCCCTCCTCG TGGCTTATAA AGAAGATGAA GTCCCAGTTC TTAAAGATGA GCTGATCCAC 
GAGTTAAAGC AGACACTGAA CGCTATCAAA TTAGAAGAGA AAGGCGTCTA CGTCCAGGCC 
TCGACCCTGG GATCTCTGGA AGCTCTCCTT GAATTTCTGA AGACATCAGA AGTGCCCTAC 
GCAGGGATCA ACATTGGGCC CGTCCACAAA AAAGACGTCA TGAAGGCTTC GGTGATGCTG 
GAACACGACC CTCAGTATGC AGTGATTTTG GCCTTCGATG TGAGAATTGA ACGGGATGCC 
CAAGAAATGG CTGATAGTTT AGGAGTTAGA ATTTTTAGTG CAGAAATTAT TTATCATTTA 
TTTGATGCCT TTACGAAATA CAGACAAGAC TACAAGAAAC AGAAACAAGA AGAATTTAAG 
CACATAGCAG TATTCCCCTG CAAGATAAAG ATCCTCCCTC AGTTTATCTT CAATTCTCGG 
GATCCGATAG TGATGGGCGT GACTGTGGAA GCAGGGCAGG TGAAGCAGGG GACACCCATG 
TGCGTGCCCA GCAAAAATTT TGTTGACATT GGAATAGTAA CAAGTATTGA AGTAAACCAT 
AAACAAGTGG ATGTTGCGAA AAAAGGCCAA GAAGTCTGTG TCAAAATAGA ACCCATCCCA 
GGTGAATCTC CCAAAATGTA TGGACGCCAT TTTGAAGCTA CAGACATTCT CGTCAGTAAG 
ATCAGCCGGC AGTCCATCGA TGCTCTCAAA GACTGGTTCA GAGACGAGAT GCAGAAGAGT 
GACTGGCAGC TTATTGTGGA GCTGAAGAAA GTGTTTGAAA TCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_024999311.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855079.1 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X4, mRNA.

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CloneID	OBa220732
Clone ID Related Accession (Same CDS sequence)	XM_024999311.1
Accession Version	XM_024999311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 5B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG GGAGTGATGA TGATGATGAT TTTAATAAAC TATCCAAAAA AGCAAAGGGG 
AAAGCTCAGA AATCAAATAA GAAGCGTGAT GCATCAGAAG AGGATGAGGA TAACAGTAAA 
AGAATTAAAG AGCGTATGAG AGCACATTCT TCTGGTGAAA GTGGTGATGA ATCAGATGAA 
TTTTTGCAAT CCAGGAAAGG ACAGAAAAAA AATCAGAAAA ACAAGCCAGG TCCTACTGTC 
GAGAGTGGAA ATGAAGATGA TGACTCGTCC TTCAAAATTA AGACAGTGGC TCAAAAGAAA 
GCAGAGAAGA AAGAGCGTGA GAGAAAAAAA CGGGATGAAG AAAAGGCAAA ACTTCGGAAG 
CTGAAAGAAA AAGAAGAGCT GGAGTCTGGT AAAAAGGATC TGGGTAAACC AAAAGAATCT 
CAGCGGAAAC CTGAGGAGGA AGCTCTGAAA TCCAAAGTGA CACTTGACTC TGGGGCAGCT 
CCTGCCTCTG AAGAGAAAGG AGAGGGTCCC ATAGGCGCAG AAGATGACAA TGAAGGAGAC 
AAAAAGAAAA AAGATAAGAA GAAAAAGAAA GGAGAAAAGG AAGAAAAGGA GAAAGAAAAG 
AAGAAAGGAC CTAGCAAAGC CACAGTTAAG GCTATGCAAG AAGCTCTGGC TAAGCTTAAA 
GAAGAGGAGG AAAGACAGAA GAGAGAGGAG GAAGAACGGA TAAAACGGCT TGAAGAGTTA 
GAAGCCAAGC GTAAAGAAGA GGAAAGATTG GAACAAGAAA AAAGAGAAAG GAAAAAGCAA 
AAAGAAAAGG AAAGGAAAGA ACGCTTGAAA AAAGAGGGGA AACTTTTAAC TAAATCCCAG 
AGAGAAGCCA GAGCCAGAGC CGAAGCTACC CTTAAACTTC TACAAGCTCA GGGTGTTGAA 
GTGCCATCAA AAGACTCTTT GCCAAAGAAG AGGCCAATTT ATGAAGATAA AAAGAAGAAA 
AAACCACCGC AGCAGATGGA AAATAAAGTT TCTGAACCAG TGGAATTAAG TGCTGCTGCA 
GAAGTTGTGG AGCAAGGACT ACCCGAAAAA GAAGAGACAC CGCCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACCGAGGA TGCTGGGTTG GATGACTGGG AAGCTATGGC TAGCGATGAG 
GAGAGAGAGA CAGAAGAAAA CACGGTGCAT ATAGAAGTAA AAGAAAACCC TGAAGAGGAG 
GAGGAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAGAGTG AAGACGAAGA GGAGGAAGAA 
GGAGACAGTG AAGGCAGTGA AGGTGAAGAT GATGATGAAA AGGTGTCAGA TGAGAAGGAC 
GCTGGGAAAA CATTGGATAA AAAGCCAAGC AAAGACGTGA GCTCAGAATC TGAGGATGAC 
TCTGACGACG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCAAAACG GAGGATTGAG 
AAACGGCGAC TTGAACACAG TAAAAATGTA AATACAGAGA AGCTGAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA TACAGGGAAG ACAAAAATTC TAGATAAGCT CCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGTATC ACACAGCAAA TTGGTGCCAC CAATGTCCCT 
CTTGAAGCTA TTAATGAACA AACTAAGATG ATTAAAAATT TTGATCGAGA GAACGTACGG 
ATTCCAGGAA TGCTGATTAT TGACACTCCG GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA CATTGCCATT TTAGTTGTTG ATATTATGCA TGGTTTGGAG 
CCCCAGACAA TTGAATCTAT CAACCTTTTG AAATCTAAAA AATGTCCCTT CATTGTTGCC 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA CGTGGCCGCT 
ACTCTGAAGA AGCAGAAGAA GAACACCAGA GATGAATTTG AGGAGCGGGC CAAGGCGATC 
ATTGTGGAAT TTGCACAGCA GGGGCTGAAT GCAGCTTTGT TCTATGAGAA TAAGGATCCC 
CGCACTTTTG TGTCCTTGGT ACCTACCTCT GCACACACTG GCGATGGCAT GGGAAGTCTG 
ATCTGCCTTC TTGTTGAGTT GACTCAGACC ATGTTGAGTA AGAGGCTTGC CCAGTGTGAG 
GAGCTGAGAG CCCAGGTGAT GGAGGTTAAG GCTCTCCCGG GAATGGGCAC GACTATTGAT 
GTAATACTGA TCAACGGGCG TCTGAAGGAA GGAGACACGA TTATTGTTCC CGGAGTAGAA 
GGGCCTATCG TCACGCAGAT CCGAGGCCTC CTGTTACCTC CCCCTATGAA GGAGTTAAGA 
GTGAAGAACC AATATGAGAA GCATAAAGAG GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAGGACC TGGAAAAAAC ATTGGCTGGT TTACCCCTCC TCGTGGCTTA TAAAGAAGAT 
GAAGTCCCAG TTCTTAAAGA TGAGCTGATC CACGAGTTAA AGCAGACACT GAACGCTATC 
AAATTAGAAG AGAAAGGCGT CTACGTCCAG GCCTCGACCC TGGGATCTCT GGAAGCTCTC 
CTTGAATTTC TGAAGACATC AGAAGTGCCC TACGCAGGGA TCAACATTGG GCCCGTCCAC 
AAAAAAGACG TCATGAAGGC TTCGGTGATG CTGGAACACG ACCCTCAGTA TGCAGTGATT 
TTGGCCTTCG ATGTGAGAAT TGAACGGGAT GCCCAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATACAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCACATAG CAGTATTCCC CTGCAAGATA 
AAGATCCTCC CTCAGTTTAT CTTCAATTCT CGGGATCCGA TAGTGATGGG CGTGACTGTG 
GAAGCAGGGC AGGTGAAGCA GGGGACACCC ATGTGCGTGC CCAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAGTAAAC CATAAACAAG TGGATGTTGC GAAAAAAGGC 
CAAGAAGTCT GTGTCAAAAT AGAACCCATC CCAGGTGAAT CTCCCAAAAT GTATGGACGC 
CATTTTGAAG CTACAGACAT TCTCGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCTCTC 
AAAGACTGGT TCAGAGACGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTGTTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855079.1
CDS	489..3749
Translation	MYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKKPPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETEENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_024999311.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999311.1

ORF Insert Sequence:

ATGTACTCTG GGAGTGATGA TGATGATGAT TTTAATAAAC TATCCAAAAA AGCAAAGGGG 
AAAGCTCAGA AATCAAATAA GAAGCGTGAT GCATCAGAAG AGGATGAGGA TAACAGTAAA 
AGAATTAAAG AGCGTATGAG AGCACATTCT TCTGGTGAAA GTGGTGATGA ATCAGATGAA 
TTTTTGCAAT CCAGGAAAGG ACAGAAAAAA AATCAGAAAA ACAAGCCAGG TCCTACTGTC 
GAGAGTGGAA ATGAAGATGA TGACTCGTCC TTCAAAATTA AGACAGTGGC TCAAAAGAAA 
GCAGAGAAGA AAGAGCGTGA GAGAAAAAAA CGGGATGAAG AAAAGGCAAA ACTTCGGAAG 
CTGAAAGAAA AAGAAGAGCT GGAGTCTGGT AAAAAGGATC TGGGTAAACC AAAAGAATCT 
CAGCGGAAAC CTGAGGAGGA AGCTCTGAAA TCCAAAGTGA CACTTGACTC TGGGGCAGCT 
CCTGCCTCTG AAGAGAAAGG AGAGGGTCCC ATAGGCGCAG AAGATGACAA TGAAGGAGAC 
AAAAAGAAAA AAGATAAGAA GAAAAAGAAA GGAGAAAAGG AAGAAAAGGA GAAAGAAAAG 
AAGAAAGGAC CTAGCAAAGC CACAGTTAAG GCTATGCAAG AAGCTCTGGC TAAGCTTAAA 
GAAGAGGAGG AAAGACAGAA GAGAGAGGAG GAAGAACGGA TAAAACGGCT TGAAGAGTTA 
GAAGCCAAGC GTAAAGAAGA GGAAAGATTG GAACAAGAAA AAAGAGAAAG GAAAAAGCAA 
AAAGAAAAGG AAAGGAAAGA ACGCTTGAAA AAAGAGGGGA AACTTTTAAC TAAATCCCAG 
AGAGAAGCCA GAGCCAGAGC CGAAGCTACC CTTAAACTTC TACAAGCTCA GGGTGTTGAA 
GTGCCATCAA AAGACTCTTT GCCAAAGAAG AGGCCAATTT ATGAAGATAA AAAGAAGAAA 
AAACCACCGC AGCAGATGGA AAATAAAGTT TCTGAACCAG TGGAATTAAG TGCTGCTGCA 
GAAGTTGTGG AGCAAGGACT ACCCGAAAAA GAAGAGACAC CGCCTCCTGT TGAACCAGAA 
GAGGAAGAAG AAACCGAGGA TGCTGGGTTG GATGACTGGG AAGCTATGGC TAGCGATGAG 
GAGAGAGAGA CAGAAGAAAA CACGGTGCAT ATAGAAGTAA AAGAAAACCC TGAAGAGGAG 
GAGGAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAGAGTG AAGACGAAGA GGAGGAAGAA 
GGAGACAGTG AAGGCAGTGA AGGTGAAGAT GATGATGAAA AGGTGTCAGA TGAGAAGGAC 
GCTGGGAAAA CATTGGATAA AAAGCCAAGC AAAGACGTGA GCTCAGAATC TGAGGATGAC 
TCTGACGACG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCAAAACG GAGGATTGAG 
AAACGGCGAC TTGAACACAG TAAAAATGTA AATACAGAGA AGCTGAGAGC TCCTGTTATT 
TGTGTACTTG GACATGTGGA TACAGGGAAG ACAAAAATTC TAGATAAGCT CCGTCACACA 
CATGTACAAG ATGGTGAAGC AGGTGGTATC ACACAGCAAA TTGGTGCCAC CAATGTCCCT 
CTTGAAGCTA TTAATGAACA AACTAAGATG ATTAAAAATT TTGATCGAGA GAACGTACGG 
ATTCCAGGAA TGCTGATTAT TGACACTCCG GGGCATGAGT CTTTCAGTAA TCTGAGAAAC 
AGAGGAAGCT CTCTTTGTGA CATTGCCATT TTAGTTGTTG ATATTATGCA TGGTTTGGAG 
CCCCAGACAA TTGAATCTAT CAACCTTTTG AAATCTAAAA AATGTCCCTT CATTGTTGCC 
CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA CGTGGCCGCT 
ACTCTGAAGA AGCAGAAGAA GAACACCAGA GATGAATTTG AGGAGCGGGC CAAGGCGATC 
ATTGTGGAAT TTGCACAGCA GGGGCTGAAT GCAGCTTTGT TCTATGAGAA TAAGGATCCC 
CGCACTTTTG TGTCCTTGGT ACCTACCTCT GCACACACTG GCGATGGCAT GGGAAGTCTG 
ATCTGCCTTC TTGTTGAGTT GACTCAGACC ATGTTGAGTA AGAGGCTTGC CCAGTGTGAG 
GAGCTGAGAG CCCAGGTGAT GGAGGTTAAG GCTCTCCCGG GAATGGGCAC GACTATTGAT 
GTAATACTGA TCAACGGGCG TCTGAAGGAA GGAGACACGA TTATTGTTCC CGGAGTAGAA 
GGGCCTATCG TCACGCAGAT CCGAGGCCTC CTGTTACCTC CCCCTATGAA GGAGTTAAGA 
GTGAAGAACC AATATGAGAA GCATAAAGAG GTAGAAGCAG CTCAGGGGGT AAAGATTCTT 
GGAAAGGACC TGGAAAAAAC ATTGGCTGGT TTACCCCTCC TCGTGGCTTA TAAAGAAGAT 
GAAGTCCCAG TTCTTAAAGA TGAGCTGATC CACGAGTTAA AGCAGACACT GAACGCTATC 
AAATTAGAAG AGAAAGGCGT CTACGTCCAG GCCTCGACCC TGGGATCTCT GGAAGCTCTC 
CTTGAATTTC TGAAGACATC AGAAGTGCCC TACGCAGGGA TCAACATTGG GCCCGTCCAC 
AAAAAAGACG TCATGAAGGC TTCGGTGATG CTGGAACACG ACCCTCAGTA TGCAGTGATT 
TTGGCCTTCG ATGTGAGAAT TGAACGGGAT GCCCAAGAAA TGGCTGATAG TTTAGGAGTT 
AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATACAGACAA 
GACTACAAGA AACAGAAACA AGAAGAATTT AAGCACATAG CAGTATTCCC CTGCAAGATA 
AAGATCCTCC CTCAGTTTAT CTTCAATTCT CGGGATCCGA TAGTGATGGG CGTGACTGTG 
GAAGCAGGGC AGGTGAAGCA GGGGACACCC ATGTGCGTGC CCAGCAAAAA TTTTGTTGAC 
ATTGGAATAG TAACAAGTAT TGAAGTAAAC CATAAACAAG TGGATGTTGC GAAAAAAGGC 
CAAGAAGTCT GTGTCAAAAT AGAACCCATC CCAGGTGAAT CTCCCAAAAT GTATGGACGC 
CATTTTGAAG CTACAGACAT TCTCGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCTCTC 
AAAGACTGGT TCAGAGACGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG 
AAAGTGTTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_003586607.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586655.1 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Price & Availability↑

CloneID	OBa23755
Clone ID Related Accession (Same CDS sequence)	XM_003586607.4 , XM_003582755.4
Accession Version	XM_003586607.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586607.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586655.1
CDS	211..3870
Translation	MGKKQKNKNEDSAKDDIDLDALAAEIEGAGAAREQEPQKAKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADREPAAVKPTENNEDEPISKQDKKKKGQKGKKQSFEDNDSEELEEKDSKSKKTAKPKVEMYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKNnullPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETEENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_003586607.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586607.4

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5B ( XM_003582755.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582803.2 Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

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CloneID	OBa23755
Clone ID Related Accession (Same CDS sequence)	XM_003586607.4 , XM_003582755.4
Accession Version	XM_003582755.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582755.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582803.2
CDS	211..3870
Translation	MGKKQKNKNEDSAKDDIDLDALAAEIEGAGAAREQEPQKAKGKKKKEKKRQDFDEDDILKELEELSWEAQGIKADREPAAVKPTENNEDEPISKQDKKKKGQKGKKQSFEDNDSEELEEKDSKSKKTAKPKVEMYSGSDDDDDFNKLSKKAKGKAQKSNKKRDASEEDEDNSKRIKERMRAHSSGESGDESDEFLQSRKGQKKNQKNKPGPTVESGNEDDDSSFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELESGKKDLGKPKESQRKPEEEALKSKVTLDSGAAPASEEKGEGPIGAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKKKNnullPQQMENKVSEPVELSAAAEVVEQGLPEKEETPPPVEPEEEEETEDAGLDDWEAMASDEERETEENTVHIEVKENPEEEEEEEEEEEEEEESEDEEEEEGDSEGSEGEDDDEKVSDEKDAGKTLDKKPSKDVSSESEDDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPVICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTRDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLICLLVELTQTMLSKRLAQCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEVPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQFIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEVNHKQVDVAKKGQEVCVKIEPIPGESPKMYGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	XM_003582755.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582755.4

ORF Insert Sequence:

ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA 
AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA 
GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG 
GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA 
GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA 
GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT 
GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG 
AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA 
GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA 
CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT 
GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG 
AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG 
GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA 
GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA 
GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG 
AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC 
ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG 
AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG 
GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA 
CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC 
GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG 
CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA 
AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA 
CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT 
GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC 
ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA 
GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA 
AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA 
GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT 
AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT 
ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA 
GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA 
ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT 
GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC 
ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC 
AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG 
TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG 
AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG 
GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA 
CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG 
ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG 
GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT 
CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC 
CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG 
CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA 
TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT 
GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC 
TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA 
GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT 
TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT 
GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT 
ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA 
GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC 
TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG 
GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT 
GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA 
GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT 
CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG 
ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EIF5B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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