rasgrf2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rasgrf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasgrf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa18810 XM_002942362.5
Latest version!
Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa33635 XM_031893539.1
Latest version!
Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OXa18810 XM_002942362.4 Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa18810
    Clone ID Related Accession (Same CDS sequence) XM_002942362.4 , XM_002942362.5
    Accession Version XM_002942362.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942362.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT
    ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG
    GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA
    TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT
    ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA
    AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA
    CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT
    CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT
    ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT
    CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA
    AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA
    GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA
    AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG
    CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT
    ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC
    AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG
    GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT
    TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT
    AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC
    ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA
    CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG
    TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT
    GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA
    TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC
    TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG
    CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC
    TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA
    GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA
    TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG
    CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT
    GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942408.2
    CDS913..4611
    Translation

    Target ORF information:

    RefSeq Version XM_002942362.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942362.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT
    ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG
    GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA
    TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT
    ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA
    AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA
    CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT
    CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT
    ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT
    CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA
    AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA
    GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA
    AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG
    CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT
    ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC
    AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG
    GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT
    TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT
    AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC
    ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA
    CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG
    TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT
    GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA
    TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC
    TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG
    CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC
    TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA
    GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA
    TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG
    CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT
    GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33635
    Clone ID Related Accession (Same CDS sequence) XM_031893539.1
    Accession Version XM_031893539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGAACA CTGAACTCGT GCAAAGTCCC TCAGATACGA
    TATGCAAGTG TGGATCGCCT GTTGGAGAGG TTAACTGACT TGCGCTTCCT TAGCATTGAT
    TTTCTCAACA CATTCCTACA CACCTATCGG ATATTTACTG CTGCATTTGT GGTGCTTGAG
    AAGCTTGCTG ATATTTACAA AAGACCCTTC ACATCAATCC CTGTCAGATC CCTGGAACTG
    TTTTTTGCTA CAAGCCAAAA CCTCAGTGAT GGGAAATCAC CACATCTCTG TCGCAAGTTC
    TCCTCACCAC CACCCTTGTC TCTTTCAAGA ACCTCTTCTC CAATCAGGGC CAGGAAACTA
    TCACTTAATT CTCCACTGAA CTCAAGAATT GGAGCTTTGG ATTTATCCAC TTCATCTAGT
    GCAAGCAGCC CTACCTCTGT TTTCAGCCCT GCCACTTCTC CACCTCCCAC AACTGCTAAA
    GTACCTCTTG ATCTCAGTAA AGGACTCTCC TCTCCAGAGC AAAGTCCCGG ATCCATTGAT
    GAGAATTCTG AAAACCCTCG TATTGATTTG TGCAACAAAT TGAGAAGAAG TATTCGAAGA
    GCTGCGGTTT TAGAATCTGT TTCACTGGAT AGGACCATTC CTGAGAGCAG CCAGTCTACA
    GATACAGCTG AAAATTCACC ATGTAGGTCC CCCTCAACTC CGCGCCATCT CCGGTACCGC
    CCAACAGGAG TGCAGGCATC TGACAACCAG CGTGGATGTT CTGTCTCCCC AGCCTCTGCC
    TTTGCCATTG CTACAGCTGG AGCTGGTCAT GGAAGTCCCC CAGGCTTTAA TAACATGGAG
    AGAATATGCG ACAAGGAGTT TATAATCCGC AGAGCTGCAA CAAACCGAGT ATTGAATGTT
    CTTCGTCACT GGGTCTCTAA GCATGCACAG GATTTTGAGT TAAACCATGA AATAAAAATG
    AATGTTGTCA ATTTGCTGGA AGACGTTCTT CGTGACCCTG ACCTGCTACC CCAAGAGAGA
    AAAGCAGCTA CTAACATATT AAGTGCCCTA TCTCAGGAGG AACAAGATGA CTGCCACCTA
    GTCTTGGAGG ACATTATAAA CATGTCAGAG AGTCCCAGCT CAGAGTGCCT GGAAACCCTA
    TCTGCTATGG AACTGGCAGA GCAGATAACC CTCTTAGATC ACATAGTGTT TAGAAGCATA
    CCATACCAAG AGTTTCTTGG TCAAGGTTGG ATGAAACCAG ACAAATCTGA GAGGACGCCA
    TATATAATGA AAACTAGTCA ACATTTTAAT GATATGAGTA ACCTTGTTGC CTCGGAGATC
    ATGAAACACC CTGATGTCCC ATCCAGAGCA AGCTCAATTG AGAAGTGGGT AGTGGTAGCG
    GATATATGCC GATGTATGCA CAATTACAAT GGAGTGCTGG AGATCACATC CGCCTTGAAC
    AGAAGTGCTG TCTACAGACT CAAGAAAACT TGGGCTAAAG TTTCTAAACA GACAAAGGCT
    CTAATGGATA AGCTTCAAAA GACTGTATCA TCTGAAGGAA GATTTAAAAA TCTTAGAGAA
    ACCCTCAAAA ACTGTAACCC CCCTTCAGTG CCCTACCTTG GAATGTATTT GACCGACCTT
    GCATTTATAG AAGAGGGTAC CCCAAACTTC ACTGAGGAGG GGCTTGTAAA TTTTTCTAAA
    ATGCGAATGA TATCGCATAT AATTAGAGAA ATACGGCAAT TCCAGCAGAC CCCTTACCGC
    ATAGAGCATC AGCCTAAGGT TACTCAGTTT CTTCTGAACA AGTCTCGCAT TCTGGATGAA
    GATAATCTAT ATGAATTGTC ATTGCGAATT GAACCGCGTC TCCCAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749399.1
    CDS914..4564
    Translation

    Target ORF information:

    RefSeq Version XM_031893539.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGAACA CTGAACTCGT GCAAAGTCCC TCAGATACGA
    TATGCAAGTG TGGATCGCCT GTTGGAGAGG TTAACTGACT TGCGCTTCCT TAGCATTGAT
    TTTCTCAACA CATTCCTACA CACCTATCGG ATATTTACTG CTGCATTTGT GGTGCTTGAG
    AAGCTTGCTG ATATTTACAA AAGACCCTTC ACATCAATCC CTGTCAGATC CCTGGAACTG
    TTTTTTGCTA CAAGCCAAAA CCTCAGTGAT GGGAAATCAC CACATCTCTG TCGCAAGTTC
    TCCTCACCAC CACCCTTGTC TCTTTCAAGA ACCTCTTCTC CAATCAGGGC CAGGAAACTA
    TCACTTAATT CTCCACTGAA CTCAAGAATT GGAGCTTTGG ATTTATCCAC TTCATCTAGT
    GCAAGCAGCC CTACCTCTGT TTTCAGCCCT GCCACTTCTC CACCTCCCAC AACTGCTAAA
    GTACCTCTTG ATCTCAGTAA AGGACTCTCC TCTCCAGAGC AAAGTCCCGG ATCCATTGAT
    GAGAATTCTG AAAACCCTCG TATTGATTTG TGCAACAAAT TGAGAAGAAG TATTCGAAGA
    GCTGCGGTTT TAGAATCTGT TTCACTGGAT AGGACCATTC CTGAGAGCAG CCAGTCTACA
    GATACAGCTG AAAATTCACC ATGTAGGTCC CCCTCAACTC CGCGCCATCT CCGGTACCGC
    CCAACAGGAG TGCAGGCATC TGACAACCAG CGTGGATGTT CTGTCTCCCC AGCCTCTGCC
    TTTGCCATTG CTACAGCTGG AGCTGGTCAT GGAAGTCCCC CAGGCTTTAA TAACATGGAG
    AGAATATGCG ACAAGGAGTT TATAATCCGC AGAGCTGCAA CAAACCGAGT ATTGAATGTT
    CTTCGTCACT GGGTCTCTAA GCATGCACAG GATTTTGAGT TAAACCATGA AATAAAAATG
    AATGTTGTCA ATTTGCTGGA AGACGTTCTT CGTGACCCTG ACCTGCTACC CCAAGAGAGA
    AAAGCAGCTA CTAACATATT AAGTGCCCTA TCTCAGGAGG AACAAGATGA CTGCCACCTA
    GTCTTGGAGG ACATTATAAA CATGTCAGAG AGTCCCAGCT CAGAGTGCCT GGAAACCCTA
    TCTGCTATGG AACTGGCAGA GCAGATAACC CTCTTAGATC ACATAGTGTT TAGAAGCATA
    CCATACCAAG AGTTTCTTGG TCAAGGTTGG ATGAAACCAG ACAAATCTGA GAGGACGCCA
    TATATAATGA AAACTAGTCA ACATTTTAAT GATATGAGTA ACCTTGTTGC CTCGGAGATC
    ATGAAACACC CTGATGTCCC ATCCAGAGCA AGCTCAATTG AGAAGTGGGT AGTGGTAGCG
    GATATATGCC GATGTATGCA CAATTACAAT GGAGTGCTGG AGATCACATC CGCCTTGAAC
    AGAAGTGCTG TCTACAGACT CAAGAAAACT TGGGCTAAAG TTTCTAAACA GACAAAGGCT
    CTAATGGATA AGCTTCAAAA GACTGTATCA TCTGAAGGAA GATTTAAAAA TCTTAGAGAA
    ACCCTCAAAA ACTGTAACCC CCCTTCAGTG CCCTACCTTG GAATGTATTT GACCGACCTT
    GCATTTATAG AAGAGGGTAC CCCAAACTTC ACTGAGGAGG GGCTTGTAAA TTTTTCTAAA
    ATGCGAATGA TATCGCATAT AATTAGAGAA ATACGGCAAT TCCAGCAGAC CCCTTACCGC
    ATAGAGCATC AGCCTAAGGT TACTCAGTTT CTTCTGAACA AGTCTCGCAT TCTGGATGAA
    GATAATCTAT ATGAATTGTC ATTGCGAATT GAACCGCGTC TCCCAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa18810
    Clone ID Related Accession (Same CDS sequence) XM_002942362.4 , XM_002942362.5
    Accession Version XM_002942362.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras-specific guanine nucleotide-releasing factor 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT
    ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG
    GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA
    TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT
    ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA
    AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA
    CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT
    CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT
    ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT
    CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA
    AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA
    GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA
    AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG
    CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT
    ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC
    AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG
    GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT
    TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT
    AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC
    ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA
    CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG
    TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT
    GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA
    TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC
    TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG
    CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC
    TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA
    GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA
    TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG
    CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT
    GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942408.2
    CDS1490..5188
    Translation

    Target ORF information:

    RefSeq Version XM_002942362.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942362.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
    AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC
    GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG
    AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT
    GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT
    CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG
    GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG
    CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG
    CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC
    CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA
    GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA
    TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT
    AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA
    CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA
    AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA
    ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC
    TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA
    TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG
    CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC
    ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT
    GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG
    GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA
    ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT
    ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA
    TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT
    TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG
    ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG
    TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT
    ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC
    CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC
    AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT
    ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG
    GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA
    TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT
    ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA
    AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA
    CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT
    CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT
    ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT
    CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA
    AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA
    GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA
    AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG
    CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT
    ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC
    AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG
    GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT
    TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT
    AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC
    ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA
    CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG
    TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT
    GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA
    TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC
    TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG
    CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC
    TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA
    GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA
    TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG
    CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT
    GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.