Rasgrf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrf2
Entrez Gene ID	114513
Full Name	RAS protein-specific guanine nucleotide-releasing factor 2
General protein information	Preferred Names RAS protein-specific guanine nucleotide-releasing factor 2 Names ras-specific guanine nucleotide-releasing factor 2 guanine nucleotide-releasing factor 2 ras guanine nucleotide exchange factor 2 ras-GRF2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	human homolog is involved in stimulating the conversion of the GDP-bound form of Ras into the active form [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053721.1	NP_446173.1	ras-specific guanine nucleotide-releasing factor 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASGRF2	XP_005618219.1
Danio rerio (zebrafish)	rasgrf2b	NP_001121705.1
Pan troglodytes (chimpanzee)	RASGRF2	XP_517672.2
Xenopus tropicalis (tropical clawed frog)	rasgrf2	XP_002942408.2
Homo sapiens (human)	RASGRF2	NP_008840.1
Mus musculus (house mouse)	Rasgrf2	NP_033053.2
Rattus norvegicus (Norway rat)	Rasgrf2	NP_446173.1
Gallus gallus (chicken)	RASGRF2	XP_004949517.1
Bos taurus (cattle)	RASGRF2	XP_002689419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001238	XP_321917.5

Gene Ontology
Bibliography

Related articles in PubMed

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Wu RF, Liao C, Hatoum H, Fu G, Ochoa CD, Terada LS
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 Jan)

Long-term potentiation in the CA1 hippocampus induced by NR2A subunit-containing NMDA glutamate receptors is mediated by Ras-GRF2/Erk map kinase signaling.
Jin SX, Feig LA
PloS one5(7)e11732(2010 Jul)

Cocaine increases Ras-guanine nucleotide-releasing factor 1 protein expression in the rat striatum in vivo.
Zhang GC, Hoffmann J, Parelkar NK, Liu XY, Mao LM, Fibuch EE, Wang JQ
Neuroscience letters427(2)117-21(2007 Nov)

Developmentally regulated role for Ras-GRFs in coupling NMDA glutamate receptors to Ras, Erk and CREB.
Tian X, Gotoh T, Tsuji K, Lo EH, Huang S, Feig LA
The EMBO journal23(7)1567-75(2004 Apr)

Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.
Arozarena I, Matallanas D, Berciano MT, Sanz-Moreno V, Calvo F, Mu?oz MT, Egea G, Lafarga M, Crespo P
Molecular and cellular biology24(4)1516-30(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data identify the Ras activator, Ras-GRF1, although not Ras-GRF2, as a susceptible target to cocaine stimulation in striatal neurons
Title: Cocaine increases Ras-guanine nucleotide-releasing factor 1 protein expression in the rat striatum in vivo.

RasGRF family exchange factors, both endogenous and ectopically expressed, are present in the endoplasmic reticulum but not in the Golgi complex
Title: Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.

The following Rasgrf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10412	NM_053721.1 Latest version!	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrf2 ( NM_053721.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446173.1 Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Price & Availability↑

CloneID	ORa10412
Clone ID Related Accession (Same CDS sequence)	NM_053721.1
Accession Version	NM_053721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ276774.1. ##Evidence-Data-START## Transcript exon combination :: AJ276774.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG TTTCCTGAGC AAAAAGGCTG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA TCAGAATGTG CTCTTTTACT TCGAAGGCGA ACAGAGCGGC 
CGCCCAGCGG GCATGTACCT CCTAGAGGGC TGCAGCTGTG AGCGGACGCC CGCCCCACCA 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCGCTGG ACAAACAGTA TTACTTCACT 
GTCCTTTTTG GTCACGAAGG TCAGAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAAGCA 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAAAAGTA CATTCATCTA GTTCAGATTG TGGAGACAGA AAAAATTGCT 
GCTAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATTGAAAG ACTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGCC CGTATCATAC CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATCAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGCAGGAGGA AGTGGAAGAC CATCGTTCAG GACTACATTT GTTCCCCCCA TGCTGAGAGC 
ATGAGGAAGA GGAACCAGAT TGTCTTCACG ATGGTGGAGG CCGAGTCAGA ATATGTGCAC 
CAGCTCTACA TCCTGGTGAA CGGTTTCCTC AGGCCTCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTCCA TCAAGGTCTA AAGGCAAGGC TGGCAAATTG GCCCACTTTG 
GTTTTAGCTG ATCTCTTTGA TATTTTGCTG CCTATGCTCA ACATTTATCA AGAATTTGTG 
CGTAATCATC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAATAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGGCAACCCT GCCTGTGAGG GAAGGATGCT GGAGACATTC 
TTGACTTACC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCT 
CACACCCCCC ATGAACACGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC AAAGCTGGAG 
GAACTGTCCA GAGTGATGCA CGACGAAGTC AGTGACACCG AAAACATACG GAAAAATCTT 
GCCATTGAGA GGATGATCGT GGAGGGCTGC GACATTTTGC TAGACACCAG TCAAACTTTC 
ATCCGACAAG GTTCTCTTAT CCAAGTACCT TCTGTGGAGA GGGGGAAACT CAGTAAAGTC 
CGCCTGGGCT CCCTGTCTTT GAAAAAGGAA GGCGAGAGAC AATGTTTCTT ATTCACGAAG 
CACTTTCTGA TCTGTACCCG AAGTTCAGGA GGAAAGCTTC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACGTTGATT GAGGAGCCGG ACACAAGTGA TGATGACACC 
AAAGGTCCTG GTCACATGTT CGGACATCTG GATTTTAAAA TCGTCGTGGA GCCTCCTGAT 
GCTGCCCCGT TCACTGTGGT CTTGCTAGCA CCTTCGCGCC AGGAGAAGGC TGCCTGGATG 
AGCGACATCA GCCAGTGCGT GGACAATATC CGATGCAATG GATTGATGAC TATAGTCTTC 
GAAGAGAATT CTAAAGTTAC TGTGCCCCAT ATGATAAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGATT TGCGGTTTCT GAGCATCGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCTGCCGT AGTGCTGGGG 
AAGCTCTCCG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGATC ACTAGAGTTA 
TTTTTTGCCA CGAGCCAGAA CAACAGAGAA CATTTGGTCG ATGGAAAATC CCCACGTCTT 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTATCCA GAACGTCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC ATCTTCCTTG AACTCAAGGA TCGGAGCCTT GGACCTGACT 
ACATCCTCAT CTTCCAGTAG TCCCACCACC ACTGTCCACA GTCCTGCTGC GTCCCCACCA 
CCTCACACTG CTGTCCCAGA GTCTGCACCA GCCGACCGAG CAGGAGACTC CACAGACATG 
TCACCTTGTA GATCTCCCTC AACCACTCCC CGGCACCTCC GATATCGCCA GCCTGGAGGA 
CAGGTAGCTG ACAGCACCCA CTGTGCTGTT TCACCTGCTT CTGCTTTTGC GATCGCCACA 
GCAGCAGCAG GACATGGGAG TCCGCCAGGA TTTAACAACG AGAGGACATG TGACAAGGAG 
TTCATCATAC GGAGAACAGC CACCAATCGA GTCCTGAATG TCCTCCGCCA CTGGGTTTCA 
AAGCACTCAC AGGATTTTGA ACTCAATAAT GAACTCAAAA TGAATGTTCT GAACTTGCTA 
GAAGAAGTTC TACGAGACCC GGACCTTCTT CCTCAAGAAA GGAAAGCCAC GGCGAACATC 
CTGAGGGCTC TTTCACAAGA TGATCAAGAT GACATCCACC TAAAATTAGA GGATATAATT 
CAAATGACAG ACTGTCCAAA GGCCGAGTGC TTTGAGACAT TGTCAGCCAT GGAGCTGGCT 
GAGCAGATCA CTCTCCTGGA CCACATTGTT TTCAGAAGCA TCCCCTATGA AGAGTTTCTT 
GGGCAGGGCT GGATGAAGCT GGATAAAAAC GAAAGGACTC CTTATATCAT GAAAACCAGC 
CAGCACTTCA ATGAGATGAG TAACCTGGTG GCCTCCCAGA TAATGAATTA TGCTGACATC 
AGTTCCCGTG CCAATGCCAT TGAGAAGTGG GTAGCAGTGG CGGACATCTG CCGATGTCTA 
CACAACTACA ATGGTGTGCT GGAGATCACC TCGGCCTTAA ACAGAAGTGC CATCTACAGG 
CTGAAGAAAA CCTGGACCAA GGTGTCCAAG CAGACAAAAG CTCTGATGGA CAAACTTCAG 
AAGACTGTCT CGTCTGAAGG AAGATTTAAA AACCTCAGAG AAACTCTCAA AAACTGTAAC 
CCCCCAGCTG TCCCTTACCT TGGGATGTAC TTGACAGACC TGGCATTCAT TGAAGAAGGG 
ACACCGAACT TTACCGAAGA AGGCCTTGTC AATTTCTCCA AAATGCGAAT GATATCACAC 
ATCATTCGTG AGATACGCCA GTTCCAGCAG ACCGCTTACC GCATAGACCA GCAGCCAAAG 
GTCATACAAT ACCTGCTTGA CAAAGCCCTT GTCATAGATG AAGATACACT CTATGAGCTG 
TCACTCAAAA TTGAACCTCG ACTCCCTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446173.1
CDS	1..3573
Translation	MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSGRPAGMYLLEGCSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHEGQKPLELRCEEEQAGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYHTHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEGNPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDTSDDDTKGPGHMFGHLDFKIVVEPPDAAPFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNREHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTTSSSSSSPTTTVHSPAASPPPHTAVPESAPADRAGDSTDMSPCRSPSTTPRHLRYRQPGGQVADSTHCAVSPASAFAIATAAAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHSQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWTKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	NM_053721.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053721.1

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG TTTCCTGAGC AAAAAGGCTG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA TCAGAATGTG CTCTTTTACT TCGAAGGCGA ACAGAGCGGC 
CGCCCAGCGG GCATGTACCT CCTAGAGGGC TGCAGCTGTG AGCGGACGCC CGCCCCACCA 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCGCTGG ACAAACAGTA TTACTTCACT 
GTCCTTTTTG GTCACGAAGG TCAGAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAAGCA 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAAAAGTA CATTCATCTA GTTCAGATTG TGGAGACAGA AAAAATTGCT 
GCTAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATTGAAAG ACTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGCC CGTATCATAC CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATCAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGCAGGAGGA AGTGGAAGAC CATCGTTCAG GACTACATTT GTTCCCCCCA TGCTGAGAGC 
ATGAGGAAGA GGAACCAGAT TGTCTTCACG ATGGTGGAGG CCGAGTCAGA ATATGTGCAC 
CAGCTCTACA TCCTGGTGAA CGGTTTCCTC AGGCCTCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTCCA TCAAGGTCTA AAGGCAAGGC TGGCAAATTG GCCCACTTTG 
GTTTTAGCTG ATCTCTTTGA TATTTTGCTG CCTATGCTCA ACATTTATCA AGAATTTGTG 
CGTAATCATC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAATAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGGCAACCCT GCCTGTGAGG GAAGGATGCT GGAGACATTC 
TTGACTTACC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCT 
CACACCCCCC ATGAACACGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC AAAGCTGGAG 
GAACTGTCCA GAGTGATGCA CGACGAAGTC AGTGACACCG AAAACATACG GAAAAATCTT 
GCCATTGAGA GGATGATCGT GGAGGGCTGC GACATTTTGC TAGACACCAG TCAAACTTTC 
ATCCGACAAG GTTCTCTTAT CCAAGTACCT TCTGTGGAGA GGGGGAAACT CAGTAAAGTC 
CGCCTGGGCT CCCTGTCTTT GAAAAAGGAA GGCGAGAGAC AATGTTTCTT ATTCACGAAG 
CACTTTCTGA TCTGTACCCG AAGTTCAGGA GGAAAGCTTC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACGTTGATT GAGGAGCCGG ACACAAGTGA TGATGACACC 
AAAGGTCCTG GTCACATGTT CGGACATCTG GATTTTAAAA TCGTCGTGGA GCCTCCTGAT 
GCTGCCCCGT TCACTGTGGT CTTGCTAGCA CCTTCGCGCC AGGAGAAGGC TGCCTGGATG 
AGCGACATCA GCCAGTGCGT GGACAATATC CGATGCAATG GATTGATGAC TATAGTCTTC 
GAAGAGAATT CTAAAGTTAC TGTGCCCCAT ATGATAAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGATT TGCGGTTTCT GAGCATCGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCTGCCGT AGTGCTGGGG 
AAGCTCTCCG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGATC ACTAGAGTTA 
TTTTTTGCCA CGAGCCAGAA CAACAGAGAA CATTTGGTCG ATGGAAAATC CCCACGTCTT 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTATCCA GAACGTCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC ATCTTCCTTG AACTCAAGGA TCGGAGCCTT GGACCTGACT 
ACATCCTCAT CTTCCAGTAG TCCCACCACC ACTGTCCACA GTCCTGCTGC GTCCCCACCA 
CCTCACACTG CTGTCCCAGA GTCTGCACCA GCCGACCGAG CAGGAGACTC CACAGACATG 
TCACCTTGTA GATCTCCCTC AACCACTCCC CGGCACCTCC GATATCGCCA GCCTGGAGGA 
CAGGTAGCTG ACAGCACCCA CTGTGCTGTT TCACCTGCTT CTGCTTTTGC GATCGCCACA 
GCAGCAGCAG GACATGGGAG TCCGCCAGGA TTTAACAACG AGAGGACATG TGACAAGGAG 
TTCATCATAC GGAGAACAGC CACCAATCGA GTCCTGAATG TCCTCCGCCA CTGGGTTTCA 
AAGCACTCAC AGGATTTTGA ACTCAATAAT GAACTCAAAA TGAATGTTCT GAACTTGCTA 
GAAGAAGTTC TACGAGACCC GGACCTTCTT CCTCAAGAAA GGAAAGCCAC GGCGAACATC 
CTGAGGGCTC TTTCACAAGA TGATCAAGAT GACATCCACC TAAAATTAGA GGATATAATT 
CAAATGACAG ACTGTCCAAA GGCCGAGTGC TTTGAGACAT TGTCAGCCAT GGAGCTGGCT 
GAGCAGATCA CTCTCCTGGA CCACATTGTT TTCAGAAGCA TCCCCTATGA AGAGTTTCTT 
GGGCAGGGCT GGATGAAGCT GGATAAAAAC GAAAGGACTC CTTATATCAT GAAAACCAGC 
CAGCACTTCA ATGAGATGAG TAACCTGGTG GCCTCCCAGA TAATGAATTA TGCTGACATC 
AGTTCCCGTG CCAATGCCAT TGAGAAGTGG GTAGCAGTGG CGGACATCTG CCGATGTCTA 
CACAACTACA ATGGTGTGCT GGAGATCACC TCGGCCTTAA ACAGAAGTGC CATCTACAGG 
CTGAAGAAAA CCTGGACCAA GGTGTCCAAG CAGACAAAAG CTCTGATGGA CAAACTTCAG 
AAGACTGTCT CGTCTGAAGG AAGATTTAAA AACCTCAGAG AAACTCTCAA AAACTGTAAC 
CCCCCAGCTG TCCCTTACCT TGGGATGTAC TTGACAGACC TGGCATTCAT TGAAGAAGGG 
ACACCGAACT TTACCGAAGA AGGCCTTGTC AATTTCTCCA AAATGCGAAT GATATCACAC 
ATCATTCGTG AGATACGCCA GTTCCAGCAG ACCGCTTACC GCATAGACCA GCAGCCAAAG 
GTCATACAAT ACCTGCTTGA CAAAGCCCTT GTCATAGATG AAGATACACT CTATGAGCTG 
TCACTCAAAA TTGAACCTCG ACTCCCTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 Jan)
Wu RF,Liao C,Hatoum H,Fu G,Ochoa CD,Terada LS

Long-term potentiation in the CA1 hippocampus induced by NR2A subunit-containing NMDA glutamate receptors is mediated by Ras-GRF2/Erk map kinase signaling.
PloS one5(7)e11732(2010 Jul)
Jin SX,Feig LA

Cocaine increases Ras-guanine nucleotide-releasing factor 1 protein expression in the rat striatum in vivo.
Neuroscience letters427(2)117-21(2007 Nov)
Zhang GC,Hoffmann J,Parelkar NK,Liu XY,Mao LM,Fibuch EE,Wang JQ

Developmentally regulated role for Ras-GRFs in coupling NMDA glutamate receptors to Ras, Erk and CREB.
The EMBO journal23(7)1567-75(2004 Apr)
Tian X,Gotoh T,Tsuji K,Lo EH,Huang S,Feig LA

Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.
Molecular and cellular biology24(4)1516-30(2004 Feb)
Arozarena I,Matallanas D,Berciano MT,Sanz-Moreno V,Calvo F,Mu?oz MT,Egea G,Lafarga M,Crespo P

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Rasgrf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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